LOCUS       42482#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21093 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 42482
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000020884
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..9186
                     /note="5 arm"
     misc_feature    9214..9238
                     /note="B1 Gateway"
     misc_feature    9251..9298
                     /note="Frt"
     misc_feature    9299..9798
                     /note="mouse En2 intron"
     primer          complement(9367..9388)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9263..9286)
                     /note="FRT3"
     primer          13464..13482
                     /note="seq primer NF"
     misc_feature    13524..13531
                     /note="RE sites"
     misc_feature    15324..15357
                     /note="loxP"
     misc_feature    complement(13532..13563)
                     /note="Rox"
     misc_feature    complement(15284..15315)
                     /note="Rox"
     misc_feature    13564..13585
                     /note="polylinker"
     CDS             14144..14740
                     /note="puromycin N-acetyl transferase"
     misc_feature    14259..14264
                     /note="Mutated RsrII site"
     intron          9799..10173
                     /note="mouse En2 intron"
     misc_feature    10171..10173
                     /note="Splice Acceptor"
     exon            10174..10227
                     /note="mouse En2 exon"
     primer          10074..10095
                     /note="seq primer FCHK2"
     misc_feature    10228..10350
                     /note="1Dleader"
     misc_feature    10351..10402
                     /note="F2A"
     misc_feature    10498..11214
                     /note="EGFP"
     misc_feature    10408..10497
                     /note="3X NLS"
     misc_feature    13280..13518
                     /note="SV40 polyadenylation site"
     misc_feature    12313..13257
                     /note="ERT2"
     promoter        13596..14107
                     /note="PGK promoter"
     misc_feature    14851..15283
                     /note="PGK pA"
     primer          13338..13361
                     /note="LRPCR RAF5"
     misc_feature    11224..11277
                     /note="T2A"
     misc_feature    11274..11274
                     /note="A to G to kill RsRII site"
     misc_feature    11278..12303
                     /note="Cre"
     misc_feature    10189..10228
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    10189..10204
                     /note="FCHK QC prefix region"
     misc_feature    10205..10222
                     /note="FCHK QC critical region"
     misc_feature    10223..10228
                     /note="FCHK QC suffix region"
     misc_feature    15380..15400
                     /note="B2 Gateway"
     misc_feature    9187..15430
                     /note="Synthetic Cassette"
     exon            15830..15924
                     /db_xref="ENSEMBL:ENSMUSE00000399803"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 1 ENSMUSE00000399803"
     misc_feature    15431..16075
                     /note="Critical Region"
     misc_feature    16099..16132
                     /note="Downstream LoxP"
     misc_feature    16076..16155
                     /note="synthetic loxP region"
     exon            19194..19285
                     /db_xref="ENSEMBL:ENSMUSE00000359527"
                     /rank="7"
                     /note="ENSMUSE00000359527"
     misc_feature    16156..21093
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaattaaa atctcaacag gtcttccgag tgagaaagaa ggaagaatga
     4321 ggatttaagc atacgctcaa atgttgatca aaatgctcac acagtccagg gccgggatag
     4381 ggatcaccgg ctgtgtttgc ctagcaaagt gccagaccct gagttccacc ctcatagggc
     4441 actaaaccaa agaaaccatg ggaaacaaac aaacgcgtac actgagctct caccactcag
     4501 ctggtctgtg tgggacctac cctgtctggg tgcgctgttt ctaagagact gcacactaag
     4561 aaacggagga aacttggtct ttgttgcttg gttagcatgt atcattatct ctggacctcc
     4621 tgcaaggcct ctctgtgtta gtcccagtca cgtccaaagc tatttctgag catgctcagt
     4681 gaaccaaccc tgccaaaggc ctaccagcac catgaggcag tgcctgcagg agaagcccaa
     4741 ccttgaccat acatctgagc atcagtgcct cccaaaaagt ccagctaggg tcaagagacc
     4801 ttttaaactt cactcaaccc agcatcttcc agaagccttt tcttctgcag ggcacccttt
     4861 tctttctgca ggaaattggg gatagaattt cctagagttc ttagttgttc tgcaaatggg
     4921 attttaaatt ccctttgggg aatttagggg attgatatca tgaaggcctg aagtggtcag
     4981 atctggtcat gccttgttgt ctgtcctgac cttggccagg cagctctctg gggcttactt
     5041 ttctcacttg caatggactt aataacagtc acattctaga attttagcct ttgcttttgt
     5101 ttgtctttgc tgccagctga gggtggaaca tggtaagacc tagacccatg tgaatccaga
     5161 agaagcagag cagacacgca cgtgtctggc ttggaccgca gaagacctgt tgggctagcc
     5221 aggaattggc ctgccgcctg tgtagtcgga attctgtatg ctggcaaggc cttggcccgg
     5281 tttttcagat gaagtcattg ttgcttaaga ggcagccgcc tttggggagt atgttttgaa
     5341 agggtaggaa ggctgggatg gcaaaggcta ctctggaaca atattcctgc ccaaggtata
     5401 gacaggaagg actttcaggt tgtatctgga gcaaaggtta gtgtccagag ctagcactga
     5461 agtgggagat gtgggagagg atcaaggata aagagagtat ggccactaac ccagaggccc
     5521 cctgagctcc tgtagatcat tgtcatcctc cagagtgccc ctgagagttc acaggtggtg
     5581 ggaaagagac ccacggtgct cggcaccctg tggtagaatg tgaaagccgc agagcccttg
     5641 gacttggtgg caaagcataa agaacagagc ttgctctgaa ttgtctctgc taagcagcct
     5701 gtagagtgta cattcccacg gctgtcttgg gtgttatgag tatggctgtc ggtgtattga
     5761 gcattctgtg gagcactggg ttctgtgggg ccctgtgatg ggctctggag acactgaggg
     5821 aaggggtcag ttagggcagc agatccaacc tcagcattga ctcattagag cagctggcag
     5881 acagggtagg ctactacatg acctgggtgg aagggtgaca tggctggagg tgacagcgag
     5941 ggtggggagg gagtggccct aaaaacccat cactgtgaca tctgttctgg ctgctggttc
     6001 ctcactttca ggaaaggctt aagatcacca gtgggaggtc tttctcttgc ccaatgctct
     6061 ctcacaatta gcaatgctat ctctaggagg cttcctgtga ccttgtttag tttttgggtc
     6121 atggtaagga caaaacttgt cctaatgcta aaaccttgat taatatctgt cagtctaatt
     6181 agaccaccat ccccaaattc cacacactgg gaggcttcca caacacactc acactctgtg
     6241 gtccaggctg gtctagaact caccatgtag ttcaggctag gctcacattc aaagaaatcc
     6301 tcctgcctta accttctgag agttgagact atggccatgc cccattatac ctggccaggt
     6361 ggttcaagtt ctaatgagga ccctgcctgg ttatgggagg ccactgtctt atatcctcat
     6421 agtctttcct tggctgcatg ttgggagaca gtgaacatgg gcagagctct cagatgtccc
     6481 ctcttcaaga acatgaatcc atcaggtgtt ctactcccag catcttagag attttctgta
     6541 aattctaaca acagtgcggt tactgtatgg ttgcagcgtg gttacacaga gagattctct
     6601 ccacagcctc tgccaagtcc aaacactctg gaaaaaaaaa aaatcaagac tgtggatctt
     6661 gtgcagggac cggtgaggag agagccttct tcccactgca gcttagctat actctgatgc
     6721 tggaggtgac agcaaaggtg gggagttgga catgatgggg agaggaactg agggctttcc
     6781 ttcataatgg gcagggtgtt gggtgcactg tttcctttct tcctgctgta atggagcgtt
     6841 gtatatcttc ctgtgggctg gaatttcccc atggagacaa tgacattcct caatagggct
     6901 gaagagtaaa tgagatgaga tgattcatgt atctgggccc aaggtaagcc tcagtccaca
     6961 tctccttatt ggtcagcacc aacgtctccg ttattttaag gcattcctgc ctgttgagta
     7021 ccagggccac ttcccacttg aatctcattt ggtttccatc ttcaaagttg ggcttccccc
     7081 agactgaatc cagcctctct gccctattcc ttccactgct ctgtggttcc caggtaaatt
     7141 agtgtccctc cctgcaccta gccacccagc ctaggaatgt gggtatcact ttactcaatg
     7201 gcccgtgtgt cattggccct acatcctgct gagtgggtcc cctcctttct acccccagag
     7261 cttctccagc ccctcttggc tttctcctct cccacatccc cattggcttc cgtccacttc
     7321 cagtgtggac tctcccactg tgtataatcc tgccctactt ctccatgtca gctccagaca
     7381 ggaaggcaca gaggtgacct cacacctcat ctgtcttcct gcctagctcc tgccctcctc
     7441 cccactccag gagcttcatc cagactccta acgttaccct cccaagtgcc caccatggtc
     7501 attctgaccc ttgaccctct cacagctgca cacggcagcc tctgtcagct tcttcagggt
     7561 ccctgtccaa ttgtagccat ggtggcttct ctgggaacct gatattgtct ctcaccattg
     7621 ccaccattgc ttacctaact gtctctgcac acatatgagc acttgggggt gcaagggaag
     7681 gttgtctgcc ctgaattccc aagagcaggg agccagactc cacacaagtt cagagggtga
     7741 tggcaggttc tctccatgtg tttaaggaca agcacacata gggaagggct ggcatgtatg
     7801 gaccccgcat gggaattccc cccaccccag gaaagggtgg tatttcgtac ctgtcattcc
     7861 agttacttgg gagactgggg gcaggaggat ggcttgaacc caggagttca ggccagagtg
     7921 ggccacacaa agaattcaga gagagagaga gagagagaga gagagagaga gagagagaga
     7981 gagagagaga gagagagaga gagagagaga gaacaaaaca ggaaggggaa ggagaagtta
     8041 taaggcccca tagctctctc tggatctgag agtataataa ttaatccctt aggccaaatg
     8101 tgggggtgca tgcctttaat ctcacccttt gagaggcaga ggcaggtgga tgtctggaag
     8161 tttgattaca gcctggtcta cagagtgagt cccagactag ctggcactgt atagtgagac
     8221 attgtctcaa aacaaaacaa gcaagctgat aagaggtgag tggtctgacc actttctcag
     8281 ggaggaacag tgactcagtt tctctcttgc ttggggtctg gggcttaaac cagtgtccta
     8341 gttccatttg tgataataaa aaagtgacaa aagctacttt agtaaagaaa gggtttcctt
     8401 tcctgacaaa ggtcacagtg catccctgaa ggaaagtcaa ggcaggaact tgaatcagct
     8461 agtcatagcc tcagtcaaga atagagagaa aataattcat gtgtacttct taggtttaat
     8521 tccctctctg cacttctaca gtccagaacc ccaaccaagg gaatggcact gcccacagtg
     8581 gactgggtct tctcacatca atgacttaat caagatgatc tacatgcaag cccacaggcc
     8641 agcctaatcc agacagtccc tcactgattc tcttcccatg tgattctaga ttgtgttagc
     8701 taacagttaa aactaagtat cacagctgag catggtggct cactgagaaa caaagacagg
     8761 caaatctttg agttcaaggc cagcctgctc tacatagtgt gttccaggcc agccagagat
     8821 acacagtgag actgtctcaa acactcaaac atataaaaac aaaacacccc taaatatcat
     8881 aatcagccag acttcgtgga ctaaaattca tcagctcctc ccatttctct actgcgcagc
     8941 tggctagggt agttaggaac atccttcttt acagtgggat ttgtttggtt ttaatgaaaa
     9001 tgcaacaggt atgcgctgga tggcgcctat ggctccaggt ggaaaagcca ggcacaagca
     9061 aactccattg tgtggctggg ctgtgagagg tcttcctgaa agtggatgat gtgggttaac
     9121 attgtcttgc tctgttgagg ctcaagaatc agggagacta tccccccaag cagctggcac
     9181 ctatgaaagg cgcataacga taccacgata tcaacaagtt tgtacaaaaa agcaggctgg
     9241 cgccggaacc gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcga
     9301 accctttccc acaccaccct ccacacttgc cccaaacact gccaactatg taggaggaag
     9361 gggttgggac taacagaaga acccgttgtg gggaagctgt tgggagggtc actttatgtt
     9421 cttgcccaag gtcagttggg tggcctgctt ctgatgaggt ggtcccaagg tctggggtag
     9481 aaggtgagag ggacaggcca ccaaggtcag cccccccccc ctatcccata ggagccaggt
     9541 ccctctcctg gacaggaaga ctgaagggga gatgccagag actcagtgaa gcctggggta
     9601 ccctattgga gtccttcaag gaaacaaact tggcctcacc aggcctcagc cttggctcct
     9661 cctgggaact ctactgccct tgggatcccc ttgtagttgt gggttacata ggaaggggga
     9721 cgggattccc cttgactggc tagcctactc ttttcttcag tcttctccat ctcctctcac
     9781 ctgtctctcg accctttccc taggatagac ttggaaaaag ataaggggag aaaacaaatg
     9841 caaacgaggc cagaaagatt ttggctgggc attccttccg ctagctttta ttgggatccc
     9901 ctagtttgtg ataggccttt tagctacatc tgccaatcca tctcattttc acacacacac
     9961 acaccacttt ccttctggtc agtgggcaca tgtccagcct caagtttata tcaccacccc
    10021 caatgcccaa cacttgtatg gccttgggcg ggtcatcccc ccccccaccc ccagtatctg
    10081 caacctcaag ctagcttggg tgcgttggtt gtggataagt agctagactc cagcaaccag
    10141 taacctctgc cctttctcct ccatgacaac caggctccca ggtcccgaaa accaaagaag
    10201 aagaacgcag atcgcagatc gcagatctct agagtcaccg agttgcttta ccggatgaag
    10261 agggccgaaa catactgtcc aaggcccttg ctggcaatcc acccaactga agccagacac
    10321 aaacagaaaa ttgtggcacc ggtgaaacag actttgaatt ttgaccttct caagttggcg
    10381 ggagacgtcg agtccaaccc cggaccggtg catgtggacc ccaagaaaaa gcggaaggtg
    10441 gaccctaaaa agaaacgcaa agtggatcct aagaaaaaaa gaaaggtgca cgtgtccatg
    10501 gtgtccaagg gcgaggaact gttcaccggc gtggtgccca tcctggtgga actggatggc
    10561 gacgtgaacg gccacaagtt cagcgtgtcc ggcgagggcg aaggcgacgc cacatacgga
    10621 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccttg gcctaccctc
    10681 gtgaccacac tgacctacgg cgtgcagtgc ttcagcagat accccgacca tatgaagcag
    10741 cacgacttct tcaagagcgc catgcccgag ggctacgtgc aggaaagaac catcttcttt
    10801 aaggacgacg gcaactacaa gaccagggcc gaagtgaagt tcgagggcga caccctcgtg
    10861 aacagaatcg agctgaaggg catcgacttc aaagaggacg gcaacatcct gggccacaag
    10921 ctggagtaca actacaacag ccacaacgtg tacatcatgg ccgacaagca gaaaaacggc
    10981 atcaaagtga acttcaagat ccggcacaac atcgaggacg gctccgtgca gctggccgac
    11041 cactaccagc agaacacccc tatcggcgac ggccctgtgc tgctgcctga caaccactac
    11101 ctgagcaccc agagcgccct gagcaaggac cccaacgaga agagggacca catggtgctg
    11161 ctggaattcg tgaccgccgc tggcatcacc ctgggcatgg acgagctgta caagcctgca
    11221 ggcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cgggccgtcc
    11281 aatttactga ccgtacacca aaatttgcct gcattaccgg tcgatgcaac gagtgatgag
    11341 gttcgcaaga acctgatgga catgttcagg gatcgccagg cgttttctga gcatacctgg
    11401 aaaatgcttc tgtccgtttg ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa
    11461 tggtttcccg cagaacctga agatgttcgc gattatcttc tatatcttca ggcgcgcggt
    11521 ctggcagtaa aaactatcca gcaacatttg ggccagctaa acatgcttca tcgtcggtct
    11581 gggctgccac gaccaagtga cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa
    11641 gaaaacgttg atgccggtga acgtgcaaaa caggctctag cgttcgaacg cactgatttc
    11701 gaccaggttc gttcactcat ggaaaatagc gaccgctgcc aggatataag gaatctggca
    11761 tttctgggga ttgcttataa caccctgtta cgtatagccg aaattgccag gatcagggtt
    11821 aaagatatct cacgtactga cggtgggaga atgttaatcc atattggcag aacgaaaacg
    11881 ctggttagca ccgcaggtgt agagaaggca cttagcctgg gggtaactaa actggtcgag
    11941 cgatggattt ccgtctctgg tgtagctgat gatccgaata actacctgtt ttgccgggtc
    12001 agaaaaaatg gtgttgccgc gccatctgcc accagccagc tatcaactcg cgccctggaa
    12061 gggatttttg aagcaactca tcgattgatt tacggcgcta aggatgactc tggtcagaga
    12121 tacctggcct ggtctggaca cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct
    12181 ggagtttcaa taccggagat catgcaagct ggtggctgga ccaatgtaaa tattgtcatg
    12241 aactatatcc gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc
    12301 gatctcgagc catctgctgg agacatgaga gctgccaacc tttggccaag cccgctcatg
    12361 atcaaacgct ctaagaagaa cagcctggcc ttgtccctga cggccgacca gatggtcagt
    12421 gccttgttgg atgctgagcc ccccatactc tattccgagt atgatcctac cagacccttc
    12481 agtgaagctt cgatgatggg cttactgacc aacctggcag acagggagct ggttcacatg
    12541 atcaactggg cgaagagggt gccaggcttt gtggatttga ccctccatga tcaggtccac
    12601 cttctagaat gtgcctggct agagatcctg atgattggtc tcgtctggcg ctccatggag
    12661 cacccagtga agctactgtt tgctcctaac ttgctcttgg acaggaacca gggaaaatgt
    12721 gtagagggca tggtggagat cttcgacatg ctgctggcta catcatctcg gttccgcatg
    12781 atgaatctgc agggagagga gtttgtgtgc ctcaaatcta ttattttgct taattctgga
    12841 gtgtacacat ttctgtccag caccctgaag tctctggaag agaaggacca tatccaccga
    12901 gtcctggaca agatcacaga cactttgatc cacctgatgg ccaaggcagg cctgaccctg
    12961 cagcagcagc accagcggct ggcccagctc ctcctcatcc tctcccacat caggcacatg
    13021 agtaacaaag gcatggagca tctgtacagc atgaagtgca agaacgtggt gcccctctat
    13081 gacctgctgc tggaggcggc ggacgcccac cgcctacatg cgcccactag ccgtggaggg
    13141 gcatccgtgg aggagacgga ccaaagccac ttggccactg cgggctctac ttcatcgcat
    13201 tccttgcaaa agtattacat cacgggggag gcagagggtt tccctgccac agcttgatga
    13261 agatctgagc tcttaattaa tgatcataat cagccatatc acatctgtag aggttttact
    13321 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    13381 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    13441 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    13501 tgtatcttat catgtctgga tccgtttaaa ctaactttaa ataattggca ttatttaaag
    13561 ttacatatgg cggccgccct aaggctcgac ctcgaaattc taccgggtag gggaggcgct
    13621 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
    13681 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
    13741 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
    13801 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
    13861 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
    13921 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
    13981 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcttccggag gcccggcatt
    14041 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
    14101 ttcgacctgc atccatctag atctcgagca gctgaagctt accatgaccg agtacaagcc
    14161 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
    14221 gttcgccgac taccccgcca cgcgccacac cgtcgatcca gatcgccaca tcgagcgggt
    14281 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
    14341 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
    14401 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
    14461 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
    14521 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
    14581 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
    14641 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
    14701 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
    14761 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg atgatatcag atccccggga
    14821 tgcagaaatt gatgatctat taaacaataa agatgtccac taaaatggaa gtttttcctg
    14881 tcatactttg ttaagaaggg tgagaacaga gtacctacat tttgaatgga aggattggag
    14941 ctacgggggt gggggtgggg tgggattaga taaatgcntg ctctttactg aaggctcttt
    15001 actattgctt tatgataatg tttcatagtt ggatatcata atttaaacaa gcaaaaccaa
    15061 attaagggcc agctcattcc tcccactcat gatctataga tctatagatc tctcgtggga
    15121 tcattgtttt tctcttgatt cccactttgt ggttctaagt actgtggttt ccaaatgtgt
    15181 cagtttcata gcctgaagaa cgagatcagc agcctctgtt ccacatacac ttcattctca
    15241 gtattgtttt gccaagttct aattccatca gaagctggtc gagtaacttt aaataattgg
    15301 cattatttaa agttaggcgc gccataactt cgtatagcat acattatacg aagttatgtc
    15361 gagatattta aatattagac ccagctttct tgtacaaagt ggttgatatc tctatagtcg
    15421 cagtaggcgg agggtaagcc aaacaactat tgaaggcaaa gttgccagtg gccacagaat
    15481 gttaccttct tggtttcaac ctctagggag agcaggcttc atagcaaact ccaggcagaa
    15541 actgccaagg gagaccatgg gactcagaga tcacctgctt cagtgtcctt ccctcagctt
    15601 tggagtgttt tgatggcaag ctggtggctc ccattggctt tgaactgctc cctcatggag
    15661 catctgcact tccagagccc atgcctattc ttcagcttct ctctgactgc ttcctcggtg
    15721 tgaggagaaa gctcagtttg tctcagcttg gcttggttct gggttccagt cttagtcctg
    15781 ggaggcagga aggctgtgtt catttgtcaa gctgtctttg ctcttccagt tgacctgaca
    15841 tccttcgtga cgcacttcga gtgggacatg gccaaatatc ccgccaagca gccactggtg
    15901 agcgtggtgg acacactggc caaggtgagg agagtctgct tgcaggcacc tgaccatcca
    15961 agatgacggg cacggggttg ggactctgac aaggtggagg gcgaagggtt gggatgctag
    16021 cccctgagtg accctatgta ggtctcctct caccatgctc tgggtaacat tagaagagat
    16081 ggcgcaacgc aattaatgat aacttcgtat agcatacatt atacgaagtt atggtctgag
    16141 ctcgccatca gttcaagacc cagacaccaa ttcagttgac tcccctactt cttttcaaaa
    16201 atgatgacgg gaagaaggct tgatctttcc tccctcagtc tgcacagggg aacccagtgc
    16261 cccttggatg attgattgat ggctgattga ttgattttgc ggtactggat atggaactca
    16321 gaatctttcc catacgaagc attataccac agcccccttt taattttgat tttgagaatg
    16381 ggtctcacta agttgaccaa gctggtcttg aaccagcttt gtaactcaag ctggacttaa
    16441 actcatgacc cttctgcatc agcctcccta gtagctgcaa ttacaggcct tgggtaccaa
    16501 gcctggctct ctcgggaaga aacctaatcg ttttagaaga cagtttggct gaggagcccc
    16561 acctctcagt catactgtgc caagtgtctt gtgataacaa caaggtagtc ccatgacagg
    16621 gtgccatctt tgaagccatg gtctccagct tgctcacact gtgtacaagc accatagcag
    16681 aggggtctgc atggaaattg tgggcatccc ataaccaagg cggcttctat gtgctttcac
    16741 catgggtaaa agctgactgt cctgccctgt tgctgatgca tacagaaccc catatatgtc
    16801 atgtctctgg ctggcctgat ggctggggag atcccagctc tactctgaac aaagctgccc
    16861 tgttctctaa gtgagtagct gagctcccct gagggcagta ggcaggtcag ggcaggaagg
    16921 gtggctttga atcttttctt cttcagattt cttgggaatc taggtgtggc tccttgggct
    16981 tacatccctt tccaagacaa tcaggcagag gcttttaaag gtatttctac aggggcagcc
    17041 tgggcattgt accaagtgtg gctttcagga gagcctcaca cctgtcccac tgagctagat
    17101 agagaataca tttttgtgag attggacacc ccaacttggg attttgagtc ccagagacac
    17161 tagacatggc tttgggggag tcagagagta gtagaatcat agaagagaac tggctcctct
    17221 ctagcccagg gttcccaaat gcacaactgt tgaagcatca aactgggaga ggatgtgggt
    17281 aggaaagcaa atcccacaag ttgcgctgtg tgcatggctc cactctccac cacaggtaac
    17341 ctgcaggcca gcaggccaga ggttgtggga gactgggtct tcagtccttg ttgccacaag
    17401 agcatgtgtc tcagccccag cctcacttgg ttcttctgag gtggtggtaa ggctttttac
    17461 agtttgtata gcagtttttc ctgtagtcaa gatggtgtgg ccgccccttg agtgtggcgt
    17521 gtggtacgat ccgagcgtat agtgtagctg tgcagccctt gtagcctagt gtgctgacgc
    17581 agtaggatgc catcagtgaa gaaaacccca cacagcacat ggatctctgc gaagcatttt
    17641 tgtaatgccc tgggaatata taaccgttct gaaaaatgtt tttaaaatgt aaggaaaaga
    17701 tcaatctcat ccccataatc aatgcaaaga caggacattg aaaatctgct actgcttcag
    17761 ttccgattcc ctttccctct ctcagtgacg ggggctgaag gttcagagag gaccgaccgc
    17821 ctgagtattt aaactcagag cttccacagt gccgtgggtt tcatctcatg atctcaacat
    17881 ctcctccatc agcatcgtac cctagatcat agacacacat tgctgggcca agcactctca
    17941 ccatagccca ctgccaaaca ttagcaagga gatggtcaga tggcttgtgt gtaaaggctg
    18001 cagtggtttt cagccttcct aatgttgcta ccctctaata cagttcctca tgttgtggtg
    18061 acccatcacc ctctaccata acgttatctt cactgttgct tcatatctgt aattttgcta
    18121 atgttatgaa ttgtaaatgt ctgatataca ggatatctgc tatgtgaccc ccatgaaagg
    18181 gtcattcaac tcccaaaggg gtcacaatcc acgggctgag aaccactggg ctaaatgcac
    18241 agttgtgaag tatccaccac tgcctgtcag ctgacatgca tccatgcata tccaatacac
    18301 acccacctat ttgtagttat ggctacaggg catggttcac tgtcatgcgg tgtctttgca
    18361 gggccagatc tctgtgagcc cttgggggag cagaactttc tttaaattag cacagagctc
    18421 agcctgtggc tttgagtcac ttgttgcttt ctggcctcag cttagttacc gtagccgtca
    18481 acaactgctc ttcactgtgg ctaactgttc ttggggctct tctaaaggtg gcattcctcc
    18541 tctgggtaga tgtggagcct aagacaggga acagaggtgc tcagtcattg tgtcctgcag
    18601 cttgggagga ggctgcagtg aagcctggag aggtggatgc ctgagaggct gaacatggaa
    18661 ggtcactatg gaagacagga tacatcactg gctgccctct aacagggcca aggcaggttg
    18721 atgtggggct tccctggtct gccattgctt gctaggaaga agcccaagct agtgttcagt
    18781 gggcaagctg tgtggctcat acctcagcac ctataaagtg aggtcttgtc cccttctgct
    18841 tccccatgtg gaaactaaga gggccagatg cttgagagtc ggaagagtca ggagagggac
    18901 aggtaagacc accctggtga gtcagtaggt tgctggtttt cttttctttt tgtttttatt
    18961 ttgtttcttc tttctttacc ctgagacagg gtttcctgtc tagttgtata gcagaggatg
    19021 atctcaaact gatcttcctg tctctgcctc ccaagtgctg gacttacagc aatgagcgcc
    19081 catggctggc ttggtcacag ttgttaagtg tccatggatg cttggtgaga tgtttgaaga
    19141 gtttcactaa ctcctcaagg tcaccaggag agtctctccc ccctccctcc cagcaactgg
    19201 cacaaatcga gacagacctg aagtcccgga cagccgccta cagcgttctg aaggccaacc
    19261 tggagaactt ggagaagaga tccacgtgcg taccatgcct actgccttct ctgtgcatgt
    19321 atgtcgtggg gttagtgtgg ggaagatacc catggctttg gctcctgagc caggtggaca
    19381 gatggagcca cacactagga acctggagta gatgaagaga gaacagagcc aggtagggtg
    19441 gctgccctgc ctgtgaacac agaggagcat ggcgtttctc ctgctcgttc cttctttcct
    19501 tcctcctgac tcctcactca gtttatgtgg ctctgaggag tctgcccagg cctctcactc
    19561 catctcctct ccaaggggag caggtagggt gggatgttag accccaactg tcttctaggg
    19621 gcagatagaa tcagcagcca cccagggtgg acagatgcag ggactcagcc ataaaactgt
    19681 ctgctggcct tataaggaca atgtattgtt caggctcctg acagaccagg ctccaatgtg
    19741 gaggaatcag tagaaaagca agcccagggc catcgtggca gagactatct tgggggtgac
    19801 agtaaacacc caggaagaaa tagttacata caaacctgga catgctcctg tgtaagaggg
    19861 aaggatgaat agggccaatt agcatgaagt agtgccccac ccttgcctct agatcctgaa
    19921 tctgaggtca gaaaagcaga acagcccagg agctcagagc cagtttccct ccttcccttg
    19981 gtactgccag ttacgtttcc ctctttagag tccctaggct agatttgcta gtcacaaaag
    20041 aaaacaaaag ttgaccattc ctaatctgaa aattcaaaat gaaaaatgct cccaaaaagg
    20101 ggggaaaggt gtttgtttgt ttgtttgttt gtttgtttgt ttgtttgttt tggtttttcg
    20161 agacagagtt tctctgtgta gccctggctg ccctggaatt cactctgtag accaggctgg
    20221 ccttgaactc agaaatctgc ctgcctctgc ctcccgagtg ctgggatcaa aggcgtgcgc
    20281 caccacgccc ggctgggggg aaagttttaa gcatcattat gattctttaa atgaaaagtt
    20341 ctgtacctga cctcatggct ctagccatgg ccaccacaca aagactgaaa ctgccatgta
    20401 agcatgcctg cagctattgt gtgagtacct tacaaagcca gtgggtttct gctttaaact
    20461 acctcctgcc tgcaagagag ctcattattc taataaaaca ttcacatgtc caaaacaacc
    20521 ccaagtccaa acagaaagga tgctgaaact gctgtccatc tgcggtgaga aagtcgggtt
    20581 cagttgcccc aaggtaacag gacatgagca aaggtggata ctagtcataa ggtgggcacg
    20641 agttgccttg agctcaggat aggtcggtcc ctctcgagcc tggagtccag aacgtggtag
    20701 actacccact cacttcacca ccaagtcata gacagcttgg cagctggcaa gatggatcag
    20761 tgggttaagg tgcctgccac ctgcatgatg atctaagttt aatctctggt tccatctgat
    20821 agagggagag aactgacttc tgcatgttgt cttctgtcat ctacacatgt tctatagcac
    20881 acgagggaca cacacacggt aaataaataa aaagtgtaat tagaaagaga attgggaagc
    20941 ctggtgatgg tggcccacgc ctttagtccc agcactcagg cagaggcagg cagatctctg
    21001 tgagtttgag gccagcctgg tctacagagt gggggcagcc agggttacac aaagagaacc
    21061 tgtctcagaa aaccaaaggg gaaaacagag aat
//