LOCUS       KO-first_condition_ready_125404:125525_MGI:107562        20692 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Arpp21 targeting project(s): 125404:125525
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 92080
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            6267..6356
                     /db_xref="ENSEMBL:ENSMUSE00001238402"
                     /rank="4"
                     /note="ENSMUSE00001238402"
     exon            5541..5582
                     /db_xref="ENSEMBL:ENSMUSE00001283030"
                     /rank="3"
                     /note="ENSMUSE00001283030"
     misc_feature    4275..10201
                     /note="5 arm"
     misc_feature    10229..10253
                     /note="B1 Gateway"
     misc_feature    10266..10313
                     /note="Frt"
     misc_feature    10314..10813
                     /note="mouse En2 intron"
     primer          complement(10382..10403)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10278..10301)
                     /note="FRT3"
     primer          14465..14483
                     /note="seq primer NF"
     misc_feature    14525..14532
                     /note="RE sites"
     misc_feature    16325..16358
                     /note="loxP"
     misc_feature    complement(14533..14564)
                     /note="Rox"
     misc_feature    complement(16285..16316)
                     /note="Rox"
     misc_feature    14565..14586
                     /note="polylinker"
     CDS             15145..15741
                     /note="puromycin N-acetyl transferase"
     misc_feature    15260..15265
                     /note="Mutated RsrII site"
     intron          10814..11188
                     /note="mouse En2 intron"
     misc_feature    11186..11188
                     /note="Splice Acceptor"
     exon            11189..11228
                     /note="mouse En2 exon"
     primer          11089..11110
                     /note="seq primer FCHK2"
     misc_feature    11229..11351
                     /note="1Dleader"
     misc_feature    11352..11403
                     /note="F2A"
     misc_feature    11499..12215
                     /note="EGFP"
     misc_feature    11409..11498
                     /note="3X NLS"
     misc_feature    14281..14519
                     /note="SV40 polyadenylation site"
     misc_feature    13314..14258
                     /note="ERT2"
     promoter        14597..15108
                     /note="PGK promoter"
     misc_feature    15852..16284
                     /note="PGK pA"
     primer          14339..14362
                     /note="LRPCR RAF5"
     misc_feature    12225..12278
                     /note="T2A"
     misc_feature    12275..12275
                     /note=" A to G to kill RsRII site"
     misc_feature    12279..13304
                     /note="Cre"
     misc_feature    11204..11229
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    11204..11219
                     /note="FCHK QC prefix region"
     misc_feature    11220..11223
                     /note="FCHK QC critical region"
     misc_feature    11224..11229
                     /note="FCHK QC suffix region"
     misc_feature    16381..16401
                     /note="B2 Gateway"
     misc_feature    10202..16431
                     /note="Synthetic Cassette"
     exon            16829..16973
                     /db_xref="ENSEMBL:ENSMUSE00001303157"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 1 ENSMUSE00001303157"
     misc_feature    16432..17128
                     /note="Critical Region"
     misc_feature    17152..17185
                     /note="Downstream LoxP"
     misc_feature    17129..17208
                     /note="synthetic loxP region"
     exon            19733..19873
                     /db_xref="ENSEMBL:ENSMUSE00001307046"
                     /rank="8"
                     /note="ENSMUSE00001307046"
     exon            18975..19034
                     /db_xref="ENSEMBL:ENSMUSE00001243829"
                     /rank="7"
                     /note="ENSMUSE00001243829"
     exon            18232..18310
                     /db_xref="ENSEMBL:ENSMUSE00001231048"
                     /rank="6"
                     /note="ENSMUSE00001231048"
     misc_feature    17209..20692
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggacctcta ggatgtgtct gaggttgcat taggagtttg gtcgttaaag
     4321 gattcggaga tcaagtttaa gaatcgaccc caattgttca ggtttttctt cttcctcctc
     4381 ttcttcttct tctcttcttc ttcttcttct tcttcttctt cttcttcttc ttcttcttct
     4441 tcttcttctt cttcttcttc tccttctcct tctccttctc cttctccttc tccttctcct
     4501 tctccttctc cttctccttc tccttctttc tccttccttc tccttccttc tccttctttt
     4561 ggtttttcga tcgttttgct tttttaagga tgccataaaa gtgcctttag aaacaaataa
     4621 taaagtcatc caaggagcat atttaatcac tgcatttgta tacaggagcc cagtgctcct
     4681 tgatagacta cctcaagctg tgtgtgtaag gactttacag atgtggcttt aggctcttga
     4741 agtcttggca caaattcctt attacatgga aagatggaga gacagctcat acatgtcaaa
     4801 gccctgtatg ctagtcatcg tagatgtgtt aaggtacaaa tcttattttc aggcaaacgg
     4861 aaacccaaga gtcctcagct gaagagtcag aacacaaaga caaattgggg gaagggagtg
     4921 ggacctggaa agagctggct ggggaagcag gggagaggca gtggaaagag ctggctggtg
     4981 aaagcagggg agaggcagtg gaaagagctg gctggggaag caggagagag gcagtggaaa
     5041 gagctggctg gtgaaagcag gggagaggca gtggaaagag ctggctgggg aagcagggga
     5101 gaggcaggat taactacaag gaagaaaaga tgcgggtgca gggaaggcac actgagcttg
     5161 taaatgctat cgattttcat tgaactgatt cacagatgtg ttagccatct caatgagcca
     5221 accttatcaa aagccagtga acatagagtc atcagaaact ttccaggttg gttcttcatt
     5281 cctgggtttg ctaggaggac aaaggactaa aagtgacagt gtcgaatgat tgggacttag
     5341 gccaggaggg tgttctgcct agtggccgta acatctttgc ttcaaccaag gatatcccat
     5401 tatcaacgat aatgacatgt tcccctttga tttttctctc cccacccctc cttctgtctt
     5461 tctctccatc tctttctggt tctctgggtt tttttgtttt ttgttttgtt tctgtttctg
     5521 tttctgcttc taccatgcag cggcgactgg cggctcagaa ccaagagagg agaaaatcca
     5581 aggtagggat ctccagggtc ccaggggagt cagcaccaca gttagaataa acaatacatt
     5641 tcccatcaga gctgaagatg tgcccatatc tcagaggctg gggtgggtcc tgcctgtccc
     5701 atacaagttg gctgtcatgc tcaccttctg tctcatgtgg atgcttctgg ctcagaatct
     5761 gaacatcctt aagtgttcct aattacagta ggagtttctg tggtgcttga cccaatccac
     5821 atccactgga gattttctct tgttgttgtt gttggctaaa atttagagca gacatttcaa
     5881 tctttcaagt gatctatacc tgaaaatgaa gagacggtgt tgctcagtgc ttagaatgcc
     5941 tccgctgtcg aatcttctcc taatttagtt ttaaaggaaa tttgccctgt cagggctttc
     6001 ttgtgatgtt ttcaagaaat gacattcatt caccctttat gcttgagcca agatcgttaa
     6061 gaactgttgc aagagttttt atgctgggca ctggctctct gatacttctc tttacccttt
     6121 gcacccagaa gcatttagca cattatatcc tggactgaga agtctgattt gggaatattc
     6181 atgcaaataa tgtgatcgac cgccatgctt tgacagtctg agaatgcttt ctgctttttt
     6241 tccccctcct taatctcaat ctgcagtcag gagcaggcaa agggaagctg accagaagtc
     6301 ttgctgtctg tgaagagtct tcagctagat ctggagggga aagtcaccag gatcaggtat
     6361 aaaagctggt actagaaggg attgcatgat gacatctctg tgtgcatgct caggtataaa
     6421 cctggtatta gatgggattg catgacggca atgcatggcc actactaatt tgacattata
     6481 atgatgtttt gcgggagaaa acaggaattg aagaacgagc attttcttgc ttcctcctgt
     6541 ttatgagaca ttctgcatag tttagaagtt tggggctggg gacataactt agcaagttaa
     6601 cggcttgcta cacaagcagt ttggcctatg tttaagccct ggaacccact caaaaaatga
     6661 acatggtgca tatacttgta atctcagcac cagggaaata aagacagaca gattgactcc
     6721 tgggattcaa gggccagttg gctcaaggcc taaccagtga gtcccaggtc cccgtgagag
     6781 actgtctcaa aaataaataa atgaataaat aaataaataa ataaataaag gcagatagca
     6841 accgaggaga aacacctgag actgacgtct ggctccacat gcacaggaac ttgtactccc
     6901 agatgtacct cccacaaata tgcaaataac ccccccacac actcatacac ccccacaccc
     6961 ccacactccc catatcttgc acaactagtt tcaatgtata tcacaggata ttgcatttgt
     7021 ttttttccag actctctgag aactgcaaat ggaaaggaac tcaaaagaat ttagattaaa
     7081 acttaactta aaataaaata aaataaaata aaaatccagt aagcacggta gtgctggata
     7141 tccaccaagg ctctctcttg ataaggctga accaaataga acccaagcat cctgtctttc
     7201 tggagacagc ttacctctca gctcccaaat gaacttgcct ctaagtaaaa caccactgtt
     7261 ggctcctagg ggatgtaaga agtagtgtct aagcaccctg gactgtggta aagtgccgga
     7321 atggacgtca ggagcttttt atttttattt tatttttttg actactgcaa aaacggtccg
     7381 gaaaagcatc tgacctggtg gcaaatttga tattagccag ctgctacata tctccaccct
     7441 tatctttgat tcccaaaact taaaattcca agaaccttca caccctcttg ttactctttt
     7501 tttttccttt ttaactatta tatttatctg cgtatggaaa acatagtctt gatgctgaaa
     7561 ttttgcttat ttttctcaga ggtcaaaggc agccaagaca aaaggcagat gatctgtctg
     7621 agcccagagg aagggaaatt gattacaaaa tcattcattt ttgtttggct ttctttggct
     7681 tctcttttat cttcccaccc ccctttctgc tttaattgaa atcctgtaat tctccttatg
     7741 gacacagaga acatggagag tgctttcttt aaaaaagaac caaaagaaaa aaaaatcctt
     7801 atagatgttt gtcctaggag tgtttccttt attagtctca ttttcttcac atgccgttca
     7861 tgtggagagg cacagacaag ctctcgtttg gtagccatta ctatgtgtca tagacagccc
     7921 cctaccccct tttttaaaac ttgggctctg agaaacgtgt ggctttcctt cagcatttta
     7981 tgtgtgcttc tatttttaac ggagatggga aaggtggaat ggtgtgaatc ccatgccttg
     8041 tactgtcaat gtagatgggt ggcttgtgat gtacagcatc tgacttgtgg gaaaccctgt
     8101 acagtgccag gctccgggag aagtaaggac ccagtcgtcc ctcactccta atgccaagca
     8161 ggggaggcca gcctctgtag atttcatttg ctctttgtct cttttgacaa ggcacaggag
     8221 tcttggtaag agccatctgt gccatagcac ccatttcaga ggcaggtcta gtggtacctc
     8281 cacacagtgg gtgaaaacgg tgctttgttc ctgctggttc tgaacccaga atacaggatt
     8341 catgttggag cagagaatta ggcccctcca gctttctgtg gactagccaa agagtggtgg
     8401 gaataggggg ttggcagaaa accaacagac acatatataa tcaactcacc cgatcttaag
     8461 aatttatacg caatgccttc actagataac actgaacact tagaaaatgt gtggactgac
     8521 tcccagagaa gtatacagta catggaatgt attgcttccc ccgtgtttgt atattcacag
     8581 tatttgggaa actgccttca ttttcctgtg tgggaaaagc tttcttgtcc cccatgttac
     8641 ttgatctcag acccacacct gatggttcag tctgtcttcg agtaaaggaa gcgtgttttt
     8701 ttttctaatg ttataatatt tactgaccag tgtgttactg caacttgaat cattctttta
     8761 ctgttgttgg gtataaactt atcctgtacc aatgtattta ttaacacttg tattttaata
     8821 ttgagcgtat caataaaaat atttaaaaat aacagattgt tttttaccaa ttctacagca
     8881 ctttttgtgt attctttcta caccatattg tatatttatg tgtagaaata tatttttttg
     8941 aacctgagta tagtatctgt caacacacag cagcaatttg tacattcggc tacagtgggt
     9001 gtctttggct tctgtttttg tatataggat ttattgccaa catgatgact tttttagatt
     9061 ccaacttaga gagggctgga tggttcgtac agttttgaag aagaaatgga accattctct
     9121 catgacccac gtgcccacaa tttctctatg agtctactga tggcctggct gttcctcaga
     9181 taccacctca gctggaaatg tcctagggat gtcctgagaa agtaaaagca aactccagcc
     9241 tctagctcct ccttgaaccc tcactgactg caacagttct ctgtccctaa tcacttgaag
     9301 aatgactaca gtagaattac agtctgattt gcatatcttg tatatgtcca cccctccccc
     9361 caaaataacc ccccaggagt tggaaaaccc aacttagtta aaaatttagg aatgacagca
     9421 tgttttatct cctcttccat atcgtggaag ttggagatct tttttgtttt gttttgcttt
     9481 ggtttggttt agtttttgtt tgtttgtttt ctctcaccac cgtggcaact tcccttcagc
     9541 tatcatttct aatgggggtc cctcctcatt ggaaacaaga ctcaccccct gaccaatagt
     9601 cgctgaagca gtgagtgtca ctaagtggtc tctcatatac cacacacaca aagccagccg
     9661 caacttccct aaaacatcat gactccaatc aacatacact gcagaaacta aaaaagccaa
     9721 acaaaacatc atgtgatgca ttctgctcca aagaagcaaa tgtgcaatca aagtaatggc
     9781 tactgcgtgt caatcaatat aagcagatca gtttccagga aaaaaaaaaa aaaaaaaaaa
     9841 aaaaaaaaca atgattagca atgtagatgt agttcaacac tgtagtctgt atcaagaagc
     9901 agcacaggag aatctgtttg gggtaaaata aactacattt accccaacat agacttatag
     9961 ataacttatg aaaacatgac aggactcacc acactttcca aaaggctatg cctttttgtg
    10021 actctgattt ataacaaaat attaatgaca ttcataaacc agccctataa ccccagatct
    10081 aagatctcag cagttacagg gttcagttcg ccttgtgtgg ggtatccttc gtggcctggt
    10141 tccatggtag gatttctact gtaaattgga tgttgggtgc tttctttctc cgagttctaa
    10201 caaggcgcat aacgatacca cgatatcaac aagtttgtac aaaaaagcag gctggcgccg
    10261 gaaccgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcgaaccct
    10321 ttcccacacc accctccaca cttgccccaa acactgccaa ctatgtagga ggaaggggtt
    10381 gggactaaca gaagaacccg ttgtggggaa gctgttggga gggtcacttt atgttcttgc
    10441 ccaaggtcag ttgggtggcc tgcttctgat gaggtggtcc caaggtctgg ggtagaaggt
    10501 gagagggaca ggccaccaag gtcagccccc cccccctatc ccataggagc caggtccctc
    10561 tcctggacag gaagactgaa ggggagatgc cagagactca gtgaagcctg gggtacccta
    10621 ttggagtcct tcaaggaaac aaacttggcc tcaccaggcc tcagccttgg ctcctcctgg
    10681 gaactctact gcccttggga tccccttgta gttgtgggtt acataggaag ggggacggga
    10741 ttccccttga ctggctagcc tactcttttc ttcagtcttc tccatctcct ctcacctgtc
    10801 tctcgaccct ttccctagga tagacttgga aaaagataag gggagaaaac aaatgcaaac
    10861 gaggccagaa agattttggc tgggcattcc ttccgctagc ttttattggg atcccctagt
    10921 ttgtgatagg ccttttagct acatctgcca atccatctca ttttcacaca cacacacacc
    10981 actttccttc tggtcagtgg gcacatgtcc agcctcaagt ttatatcacc acccccaatg
    11041 cccaacactt gtatggcctt gggcgggtca tccccccccc cacccccagt atctgcaacc
    11101 tcaagctagc ttgggtgcgt tggttgtgga taagtagcta gactccagca accagtaacc
    11161 tctgcccttt ctcctccatg acaaccaggc tcccaggtcc cgaaaaccaa agaagaagaa
    11221 cgcagatctc tagagtcacc gagttgcttt accggatgaa gagggccgaa acatactgtc
    11281 caaggccctt gctggcaatc cacccaactg aagccagaca caaacagaaa attgtggcac
    11341 cggtgaaaca gactttgaat tttgaccttc tcaagttggc gggagacgtc gagtccaacc
    11401 ccggaccggt gcatgtggac cccaagaaaa agcggaaggt ggaccctaaa aagaaacgca
    11461 aagtggatcc taagaaaaaa agaaaggtgc acgtgtccat ggtgtccaag ggcgaggaac
    11521 tgttcaccgg cgtggtgccc atcctggtgg aactggatgg cgacgtgaac ggccacaagt
    11581 tcagcgtgtc cggcgagggc gaaggcgacg ccacatacgg aaagctgacc ctgaagttca
    11641 tctgcaccac cggcaagctg cccgtgcctt ggcctaccct cgtgaccaca ctgacctacg
    11701 gcgtgcagtg cttcagcaga taccccgacc atatgaagca gcacgacttc ttcaagagcg
    11761 ccatgcccga gggctacgtg caggaaagaa ccatcttctt taaggacgac ggcaactaca
    11821 agaccagggc cgaagtgaag ttcgagggcg acaccctcgt gaacagaatc gagctgaagg
    11881 gcatcgactt caaagaggac ggcaacatcc tgggccacaa gctggagtac aactacaaca
    11941 gccacaacgt gtacatcatg gccgacaagc agaaaaacgg catcaaagtg aacttcaaga
    12001 tccggcacaa catcgaggac ggctccgtgc agctggccga ccactaccag cagaacaccc
    12061 ctatcggcga cggccctgtg ctgctgcctg acaaccacta cctgagcacc cagagcgccc
    12121 tgagcaagga ccccaacgag aagagggacc acatggtgct gctggaattc gtgaccgccg
    12181 ctggcatcac cctgggcatg gacgagctgt acaagcctgc aggcgagggc agaggaagtc
    12241 ttctaacatg cggtgacgtg gaggagaatc ccgggccgtc caatttactg accgtacacc
    12301 aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg
    12361 acatgttcag ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt
    12421 gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg
    12481 aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc
    12541 agcaacattt gggccagcta aacatgcttc atcgtcggtc tgggctgcca cgaccaagtg
    12601 acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg
    12661 aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca
    12721 tggaaaatag cgaccgctgc caggatataa ggaatctggc atttctgggg attgcttata
    12781 acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg
    12841 acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg
    12901 tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg
    12961 gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg
    13021 cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc
    13081 atcgattgat ttacggcgct aaggatgact ctggtcagag atacctggcc tggtctggac
    13141 acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga
    13201 tcatgcaagc tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaacctgg
    13261 atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg cgatctcgag ccatctgctg
    13321 gagacatgag agctgccaac ctttggccaa gcccgctcat gatcaaacgc tctaagaaga
    13381 acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg gatgctgagc
    13441 cccccatact ctattccgag tatgatccta ccagaccctt cagtgaagct tcgatgatgg
    13501 gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg gcgaagaggg
    13561 tgccaggctt tgtggatttg accctccatg atcaggtcca ccttctagaa tgtgcctggc
    13621 tagagatcct gatgattggt ctcgtctggc gctccatgga gcacccagtg aagctactgt
    13681 ttgctcctaa cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga
    13741 tcttcgacat gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg
    13801 agtttgtgtg cctcaaatct attattttgc ttaattctgg agtgtacaca tttctgtcca
    13861 gcaccctgaa gtctctggaa gagaaggacc atatccaccg agtcctggac aagatcacag
    13921 acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag caccagcggc
    13981 tggcccagct cctcctcatc ctctcccaca tcaggcacat gagtaacaaa ggcatggagc
    14041 atctgtacag catgaagtgc aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg
    14101 cggacgccca ccgcctacat gcgcccacta gccgtggagg ggcatccgtg gaggagacgg
    14161 accaaagcca cttggccact gcgggctcta cttcatcgca ttccttgcaa aagtattaca
    14221 tcacggggga ggcagagggt ttccctgcca cagcttgatg aagatctgag ctcttaatta
    14281 atgatcataa tcagccatat cacatctgta gaggttttac ttgctttaaa aaacctccca
    14341 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
    14401 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    14461 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    14521 atccgtttaa actaacttta aataattggc attatttaaa gttacatatg gcggccgccc
    14581 taaggctcga cctcgaaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
    14641 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
    14701 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
    14761 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
    14821 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
    14881 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
    14941 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
    15001 ggcggggcgg gcgcccgaag gtcttccgga ggcccggcat tctgcacgct tcaaaagcgc
    15061 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg catccatcta
    15121 gatctcgagc agctgaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
    15181 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
    15241 acgcgccaca ccgtcgatcc agatcgccac atcgagcggg tcaccgagct gcaagaactc
    15301 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
    15361 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
    15421 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
    15481 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
    15541 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
    15601 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
    15661 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
    15721 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
    15781 agcgcacgac cccatgcatc gatgatatca gatccccggg atgcagaaat tgatgatcta
    15841 ttaaacaata aagatgtcca ctaaaatgga agtttttcct gtcatacttt gttaagaagg
    15901 gtgagaacag agtacctaca ttttgaatgg aaggattgga gctacggggg tgggggtggg
    15961 gtgggattag ataaatgcnt gctctttact gaaggctctt tactattgct ttatgataat
    16021 gtttcatagt tggatatcat aatttaaaca agcaaaacca aattaagggc cagctcattc
    16081 ctcccactca tgatctatag atctatagat ctctcgtggg atcattgttt ttctcttgat
    16141 tcccactttg tggttctaag tactgtggtt tccaaatgtg tcagtttcat agcctgaaga
    16201 acgagatcag cagcctctgt tccacataca cttcattctc agtattgttt tgccaagttc
    16261 taattccatc agaagctggt cgagtaactt taaataattg gcattattta aagttaggcg
    16321 cgccataact tcgtatagca tacattatac gaagttatgt cgagatattt aaatattaga
    16381 cccagctttc ttgtacaaag tggttgatat ctctatagtc gcagtaggcg gaagcctttt
    16441 ttagacttga cagtggctcc aatatgaaca gattaaagga tcctccctac acacactaca
    16501 catttaaaaa taaacctgta gggaaaaata agagttgtga tctacaggag tttagcttga
    16561 ttaaagttaa tattcatgag gcaaagttta atactagata tgtcttctga cctcccattg
    16621 acttctggat ttcagttcac cgtaaagggc aagtattctt tttttttttt ttttttttcc
    16681 tgacaggctt aattttttaa accacttaag aaagaggaat ccatacgaac ctctcatttt
    16741 tcaatacctc gtaacctctg tgcagaatgg aagccaggca gggatgatta cttctggccc
    16801 taaattatga tattttttcc cccaacagga atcaattcac ttacagcttt ccagtttccc
    16861 cagcctgcaa gaggaggata aatctaggaa ggatgattct gagagagaaa aagaaaagga
    16921 taagaacaga gagaaactct ctgagagacc caagatcaga atgttatcaa aaggtgaatc
    16981 tgcaaaacat ttccttacct tgcttatcat ggacactcgc aaagccaaga gacattgcaa
    17041 acccacacca atgtttacag cccagggcac tctgcaccta attttccaag tggccctata
    17101 tgtgtaagag agaaaaaaat agcgacctga gatggcgcaa cgcaattaat gataacttcg
    17161 tatagcatac attatacgaa gttatggtct gagctcgcca tcagttcagg agaaagaggg
    17221 aattgaaatc ggttcctgta ttcaggttca ctgacaactg acttggattg gcgaacaatg
    17281 gtgatacaca gctttctatt ttcgctctca cccttatctt aaaagacagg cagcaagggc
    17341 cttagcacct cctcgtaata agccacaccg ggtggaggtc aatagaagcc ctggcaccaa
    17401 gggcttcagt ctctgcatct ggccactgct gcatgttgtt tcatttgaaa acataaatca
    17461 catttctatt tggaaaagag ccattcctcc taaaaactat agacctacag tccagatttt
    17521 tagactgtga atgtacctct aattgtacaa agtagaaaaa agccaagctg gaatggagag
    17581 ctatactgga gaccacactg ggaatccaag cagcctagga atggtagcca ctctttgtgc
    17641 tctgtaaaca tattcctgct tggagcagtt ctgtctagtt gaaaccctca ctcaagcttg
    17701 gggtgtgtaa gaggaaagag cacaacatgt ctaaaatgac tgtataaacc tgtatttatc
    17761 caggaccagg atataagaaa aactcaagaa catttatctc ttgccatgtt ttatactgat
    17821 taatatttag gtagttttat aatcaaaact tcatttttaa aaactgttct gttttaattt
    17881 aaaaaaaaaa aaggttgtca tgggatcaca agctcttgag gtctctgtgg gttggatgta
    17941 cctgtcatgt ggaggcagta ccccactggt tttctgcaca gaacacactc caccaccaac
    18001 agcaagcaca cttcccctcc tgtgacacta gggtcttccc acagtcaagg tctcaaccct
    18061 caagtcttat ttctgatgtt atgatccctg cttgcaagta ttttctccct ggtaccttcc
    18121 tttgaaattt acctgagctg gaaggaaacc agaacgtttt caaaccctgc cccaccctgt
    18181 attcccatca tccatctggg caactcaact ccctcctgct gtttgtttca gattgcagcc
    18241 aagaatacac agattctaca ggcatagact tacatgggtt tctaattaac acgctgaaga
    18301 acaattccag gtaaatttaa atggtgaaac aagctttttg ctggtgttgt gggagggtgg
    18361 ggagtcagaa agaatattac aaaataatta tgattaatca gaatactttt aagaaatcaa
    18421 atactaaaaa cagatggctt ccttgatgtc tctcctgagc ttgctctgct ctgttgattg
    18481 tatctccttg tggatggcta gccctaatag tcacatagtt gctccctgta taagttagag
    18541 tgtaccaact ttgctttgct aaccccaggg gcatattgaa gtaaaaagga gagaatgaaa
    18601 tgatgtttat ttacatcagt ccatggtgcc tgggtgcagg cacatagtca tgtgtactca
    18661 gggctccagc ttggcttctt tccagtttct ttcaacttta agtctttgtt ggttgtatta
    18721 ttatcattca gaaatttaca tatcatgatt atcattcgaa tgatgacatt taacactacc
    18781 aaatacttaa caagggagag gaaaaatgaa acttctgagt tcaaagtcgt ggcatgcaaa
    18841 ccaaggagtc aacatagtcc gaagatgaat tagagttagg tctgctctgt ttccccgttg
    18901 agtttgataa attgtatttg ctctttgaaa actaaaagta catgaaactt ttcctttgaa
    18961 catttatttt tcagggacag gatgatactc ttgaaaatgg agcaggaaat gattgatttc
    19021 attgctgaca gcaagtatgt tcaatggcaa tgctagctaa ttcgatgaca tgtcttagct
    19081 cagtgtctga gttaatgact cattccacag agagctgtag ataaataaaa tgtcagctcg
    19141 tatggttctt ggtatgctca atgaagaagt cactggatct tggagtggtc attgcttgga
    19201 tcacgtgtga tcttgcctgt taaatttctg gcaaaatagc cataaacagg gggcttgctt
    19261 agcccccact aatttgcatg gaggagctgt aacaagaagg gacccttctc gtttttatta
    19321 tagtttttta atgggctcca gctggctgca gtcagttatg cctggaaata attgtgctgc
    19381 gtttggtgta aactgggtaa tgacacatgt ccagatagat atctgcaatc tctattatgt
    19441 catggagcct gtcccctgcc tgcctctgtt gataaggtat cctgaactaa cccagaacac
    19501 tttgcaacag acaaaatgaa catgcggctg agaaacagca atgcctactg cagctcgatc
    19561 aataaatcta tgatagtaga gatttgtctg cgagagaaga catcaggtgg tgtcgaaatg
    19621 aaagatgcca agtgtttctg gataaaagga gatagtgacg tgtaaatccc tgtaaaatat
    19681 actactggca tgaaatgaag atgctgattt tatttctgta actctgttcc agtaaccact
    19741 ataaaaagtt cccccagatg tcatcctatc aaaggatgct ggtccatcgg gtggcagcgt
    19801 actttggatt ggaccacaac gtggatcaaa ctggaaaatc tgtcatcatc aacaagacca
    19861 gcagcaccag gatgtaagtc ccaacgtagt gtctgtttgg tgttttgttt ttctgtcaga
    19921 gactggttta taagagcaca ctattcattg taaagtatgc gacgttaaat atgtatagcg
    19981 ctggttatct ttctggccta atagcagtgg gatgtcagga atccatataa taccaaatga
    20041 gatctaggga atttggtggg agaacttcac ttcaaaatag cattcagatt aaaaacaaca
    20101 ggcattgatt ggtctgggag tgtagctcag tggtagagaa catggatggc acacacaaag
    20161 tttattagat tcccagtgca acaaatgggt aaagcctacg caagcaagca agcaagcaag
    20221 caagcaggca ggcaggcagg caggcaggca ggcaagctct ttctgcagag acaggcatga
    20281 tagcatatcc ctgtgctccc agtactctgg acactcaggc aggaggatca ggagttgaaa
    20341 aatagtccta tatacatgct atgattctgt caaaaagacg gggaggggag atgaagggag
    20401 ggtggatcaa ggaggggatg ggttagggga ggaggaatca aggataggat ttataaagct
    20461 gttacacaga gacatttgtc cttttctacc ccacccccct ttttgatgtg ggggagacat
    20521 gtgttttggg gaaagctcag aacaggatac agaaaccttg cttctcaatc ctggcatact
    20581 gactgggcat tccagtctgc tgcagtcctg atttcccatc ttggtttctt cacctgtgag
    20641 acagataagt tttagcccta agaagacaac acttggtttt tctcaagatc at
//