LOCUS       208565#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20982 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 208565
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000023593
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8470..8700
                     /db_xref="ENSEMBL:ENSMUSE00001281213"
                     /rank="2"
                     /note="ENSMUSE00001281213"
     misc_feature    4275..9885
                     /note="5 arm"
     misc_feature    9913..9937
                     /note="B1 Gateway"
     misc_feature    9950..9997
                     /note="Frt"
     misc_feature    9998..10497
                     /note="mouse En2 intron"
     primer          complement(10066..10087)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9962..9985)
                     /note="FRT3"
     primer          14163..14181
                     /note="seq primer NF"
     misc_feature    14223..14230
                     /note="RE sites"
     misc_feature    16023..16056
                     /note="loxP"
     misc_feature    complement(14231..14262)
                     /note="Rox"
     misc_feature    complement(15983..16014)
                     /note="Rox"
     misc_feature    14263..14284
                     /note="polylinker"
     CDS             14843..15439
                     /note="puromycin N-acetyl transferase"
     misc_feature    14958..14963
                     /note="Mutated RsrII site"
     intron          10498..10872
                     /note="mouse En2 intron"
     misc_feature    10870..10872
                     /note="Splice Acceptor"
     exon            10873..10926
                     /note="mouse En2 exon"
     primer          10773..10794
                     /note="seq primer FCHK2"
     misc_feature    10927..11049
                     /note="1Dleader"
     misc_feature    11050..11101
                     /note="F2A"
     misc_feature    11197..11913
                     /note="EGFP"
     misc_feature    11107..11196
                     /note="3X NLS"
     misc_feature    13979..14217
                     /note="SV40 polyadenylation site"
     misc_feature    13012..13956
                     /note="ERT2"
     promoter        14295..14806
                     /note="PGK promoter"
     misc_feature    15550..15982
                     /note="PGK pA"
     primer          14037..14060
                     /note="LRPCR RAF5"
     misc_feature    11923..11976
                     /note="T2A"
     misc_feature    11973..11973
                     /note="A to G to kill RsRII site"
     misc_feature    11977..13002
                     /note="Cre"
     misc_feature    10888..10927
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    10888..10903
                     /note="FCHK QC prefix region"
     misc_feature    10904..10921
                     /note="FCHK QC critical region"
     misc_feature    10922..10927
                     /note="FCHK QC suffix region"
     misc_feature    16079..16099
                     /note="B2 Gateway"
     misc_feature    9886..16129
                     /note="Synthetic Cassette"
     exon            16506..17460
                     /db_xref="ENSEMBL:ENSMUSE00000131552"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000131552"
     misc_feature    16130..17682
                     /note="Critical Region"
     misc_feature    17706..17739
                     /note="Downstream LoxP"
     misc_feature    17683..17762
                     /note="synthetic loxP region"
     misc_feature    17763..20982
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaatttcc ccatggaaaa gattggtgct tcttagattc ttgagttgaa
     4321 gttttcagga atatgattgc tggagctgta agaagggcag agttgacata agacacacaa
     4381 tattaaaagt ctcagagcaa tggcgggcaa gatgactcag tgagtaaaga tgctgaacct
     4441 aatggcctga gttcattccc caggacacag ttggtagaag gagagaactg acttccacag
     4501 gtgttctcta acttctgtgg gtacacacac acacacacac acacacacac acacaccaca
     4561 ccactacaca ccacacacca caccactaca caccacacac acacacacac acaccacacc
     4621 atacacacac acacacacac acacacacac acacacacac acacacacac acacacacat
     4681 taaccaacca accaaccaac caaccaacca atagactgac tgactgactg actgactgac
     4741 tgactgtgat aagaaagttc cagggcaagc tctgcttcat tttctttgta gcagtttctg
     4801 agactgggtt tttgatcact ccagattcag tagatggcga cagaggcact cagagacatt
     4861 atgagccaat ctagggcaga ctcctgctct ccaagagcaa tgctagggca gttgtttctg
     4921 gctcactcct ccccacacct gccagttcca atttgtgtac ggtcaaacta gaattgggta
     4981 tttaaagaat gatgctccaa ggcaacaggg agtgcaaggc tgaatttaat caaaggcatt
     5041 taaaatgatc ttatgccaca gtttatagca ccattaaaag aaagtcacag gtacagcaaa
     5101 gagtgatatt atatagagag agtgaaaata caaaccaggg taataccaaa tccttccaag
     5161 aataagagct gtttattgct tggaatcaag gaaaatgtca acagaactaa gcaaataaag
     5221 gacagactcc ctgtacacct gggaatgagg acagacagac agaaggatga tctgcaccta
     5281 gagggcaagt cacagcagtg tgacgtaaga agacaaaatg gtgacacacg ctcacacaat
     5341 cgcctgcaga gatgttaata agggacggga tgtagctcgg tggcagagca gttgcctcga
     5401 agagatgagt agagaggccc tgggtttgag tctcagcact gtgggtgcag agaacaataa
     5461 cctccacata aaaccagcca aacgttacag gaactgggcc cgctggtaca cccacgtaat
     5521 cccacatcag ggaggccaac gcacgaggag taccctgagg gtgaggccaa tttgggccat
     5581 agacaaaggc tctatattaa aaaagggtaa agcttaaaaa gaaaagtcta tgtagtggtg
     5641 cttgttttgt aatcccaaca cttgagaggt ggcaatgagg ccatcatgac ttcaaggtca
     5701 tcttaaatcc agtgatggag tctttgaggc ttgactgggt tccatgaaac tcattctcaa
     5761 aatagtgctc ccccaaaaga ccccaaatca aaacatatcc aaggcacaac agtgcagaag
     5821 aaacactttg tgaccccagt aaattaactg acaggaactt aacgaggatc ttagaaatga
     5881 ctgaagaatg aagatcattt attttgctca ctaaatttaa cagttaaaac agatgttttc
     5941 ttttgctttt gttttggttt ttggtttttg agacagaaaa tccatatcac tctgactgtc
     6001 ctggaagtca ctatgcagac caggctggtt tcaaactcac aaagatacac ttgcctctgc
     6061 ctcccaagtg ctgggattaa aggcttgtgt catcctgctt ggctagtagt acacttttta
     6121 aaactgagac tacccaaagc tggcaagagt aattcagcta ggcctgctta tttttttctt
     6181 tgctgtgctg aggaaggaat ccaggtcctt gattatgcga gggcagtgcc ctgctacaga
     6241 gtgacaccct cagcccgaga taggtctttg tgtgcataca ctcatgttct cttgggtggc
     6301 agcatcgttt cttgtaatgt tggtatgtat gttaaatgac tttaaagaga ggctgagaga
     6361 gagacagctc agcagtttag agagcatgtt actcttccag aggatctgag ttcggttccc
     6421 agcactgaaa gtgggcagct cacaactgac tggtgcttca ctcctgtaca cagatcacac
     6481 acacacacac acacacacac acacacacac acacactcga atgaaaataa aattaaatct
     6541 ttttaaaaga cttaaaaacg tttaggtggg ctggagaaac agctcacaat taagaccttg
     6601 cacttgcttt tccagaggac tcaggtttgg gtcctagtac ccacatggtg cctcacaacg
     6661 atccataact ccagttccag gaaatcctct tcggggcctc cttgggctca cacacacaca
     6721 cacacacaca cacacacaca cacacacaca cacacacaca cactttctct ctctctctct
     6781 tctcacaatc gcacacacag aatcattcac acacacataa attaaaaaca aaatgtcttt
     6841 tttttacaaa aaggttaatc agggcatagt ggcatatgcc tttaattcct ccacttggga
     6901 gacagaggca agtggatctc tgagttctag gtcagcctgg actgcagagc aagttccaga
     6961 gcagtcaagg ttacaacaga gaaaccttgc ctcaaaaacc caagaggaca aaaagtttag
     7021 atttctgggc ccaatcattt atatctggga atatatcata atagaaagat aagtttttta
     7081 gaaaagctgc ctgcctgaaa acagctttga ttgattgatt ctttttaaat caatacatat
     7141 catttgttgc agattataaa atattttgaa gtagacataa gctaaaaaat agaaaccaag
     7201 tttaaaagaa ctacagatat tttggccgta tccactctat ggactggcaa tgcatatatt
     7261 taaaatgatc ataaaagcta gaaaatgaca aggctgaaac acaggagaag atctttaggt
     7321 gaaaagtgat ttttgccatt ttaaagaata aacagattct ttggaattag agttgctttt
     7381 cccctgtagt ctccaaagag tttctgtgta ggtgtgtgtg tgtgtgtgtg tgtgtgtgta
     7441 tgtgtgtgtg tgtgttaatg catgcacaca agtgctcaca agtgcgcaca tgtgtatatg
     7501 aaaacgaggg agaaaaggcg agcttgggag tggggaggtg gttagatttg agtggaacaa
     7561 gcagaggaac ccaggaccgg atcctgggat attctgcgtg tgtggtcctg cactcaggag
     7621 ggggagactg gaatcaggcc atccctgcca cctgtggatt tacaattctc aggctgaaag
     7681 gagactgatt gcaggagatt ttgctactca ttgcagattt cagaggagga tgtctgagtg
     7741 tgatggtttg tatatgcgtg gtggcccagg aaatggcact attaggaggt gtggccttgt
     7801 tggagtaggg ttgtcgctgt gggtgtgggc tttaagaccc aagtcctacc tgcttggaag
     7861 ccagtcttcc actagcagcc tttagaagaa gatgtagaac tctcagctcc tcctgcacca
     7921 taccttcctg gatgctgcca tgttcccatc ttgatgataa tggactgaac ctctgaacct
     7981 gtaagccagc cccaattaaa aagtgtcctt ataagagttg ccttggggat tggagagatg
     8041 gcttagctca gagatggtta agaacactga ctactcttac aaaggtcctg aattaaaatc
     8101 ccagcaacca catggtggct cacaaccatt cataatgaga tctgacgtcc tcttctggtg
     8161 catctgaaga cactacagtg tacttacata taataaataa ataaggacat aaagtttaaa
     8221 agaaaaaaaa aagagttgcc ttggtcgtgg tgtctgttca cagcagtaaa accccaacta
     8281 agacactgat gaagacctcc tgggagagtg agggctgggt agccactgaa ggctctctgg
     8341 gagaggcgag tatgtagatg cagatctttg gagtggattc cacttagcta tataggacat
     8401 gatgcaggtc ctgattggat gtgccatgtg agtctgcctt tcccatgact attcacctgt
     8461 caatttcagg gctcaggatg ctactgttgc aagctctcct gttcctctta atcctgccca
     8521 gtcatgccga agatgacgtt actacaactg aagagctagc tcctgctttg gtccctccac
     8581 ccaagggaac ttgtgcaggt tggatggcag gcatcccagg acatcctggc cacaatggca
     8641 caccaggccg tgatggcaga gatggcactc ctggagagaa gggagagaaa ggagatgcag
     8701 gtatgtagaa gagtcctgaa tttggggggg gggtctttgc agggtgagtc cagtgattaa
     8761 ttaaggccta gacacagaca caagaaagag gctaaacctt ttttatttat ttattgtttt
     8821 atggttcata ctcatcttgg agcttgcact gtcctccttg agtgggacta gatggtacct
     8881 gctgaggtat ccatgacttc actccatctg catctctttc tctctgtcta ccccttccat
     8941 actaccaaca taatcctatt tttgttttct ccgtaattct tgttctatct attggcaatc
     9001 ataagcgctt ttcacgcatt tctaaagcac tcagtaccat gatttagtca agaagaagag
     9061 tgtaatgaat tgccctaatg ttgcttatat tctaatgcta atttgggacc cctcaagact
     9121 gtgccccaga agtgagagag tctgcatgta agactccgag tgaccctgct cccaagttat
     9181 ttctgattgg taaataaaga tgccaatggt caatagctgg gcagaaacga cacaggtaga
     9241 gtttaggttt ctcgtgcttg ggttcagagg aggtgaagaa gaagaatgtg aaggagcagc
     9301 aaggaagtgg ccatggggta agagttgaga gcatggccct gaaggctgcc caactgaagc
     9361 caagagcagc tcagatgcaa catagtaata acttggggtt atcaatagga aagttgataa
     9421 tagcacagag ggtaggtatc tgcccagctc ttgtgctgtt taaagcttgt tgtaaatatg
     9481 aaggcagtaa gtgtgtctct catctgagaa cttagtaatc aaaggtgggg tagaaaccct
     9541 gggctggaat ttaaataatt tttacaacag acaagactct gggtaatggt tactaagaga
     9601 agatgtcttc caacatgcaa catgctcatc tgaatgtgtg cagatgctga aggcatggga
     9661 tacagatagg gtgtactcag tgaggatgca gattaacaaa taggctaatc tgaggaggta
     9721 gggcttgagc cacgcctcaa aaggatggtt tccagagggt agagggggga ggacatctgg
     9781 acacagggga tagaaggcaa cacagtggtt ggtcagtgca caaacagctg gcagggtagg
     9841 gtgactatat ggctacttac aggagattac tttctggaaa tctgcaaggc gcataacgat
     9901 accacgatat caacaagttt gtacaaaaaa gcaggctggc gccggaaccg aagttcctat
     9961 tccgaagttc ctattctcta gaaagtatag gaacttcgaa ccctttccca caccaccctc
    10021 cacacttgcc ccaaacactg ccaactatgt aggaggaagg ggttgggact aacagaagaa
    10081 cccgttgtgg ggaagctgtt gggagggtca ctttatgttc ttgcccaagg tcagttgggt
    10141 ggcctgcttc tgatgaggtg gtcccaaggt ctggggtaga aggtgagagg gacaggccac
    10201 caaggtcagc cccccccccc tatcccatag gagccaggtc cctctcctgg acaggaagac
    10261 tgaaggggag atgccagaga ctcagtgaag cctggggtac cctattggag tccttcaagg
    10321 aaacaaactt ggcctcacca ggcctcagcc ttggctcctc ctgggaactc tactgccctt
    10381 gggatcccct tgtagttgtg ggttacatag gaagggggac gggattcccc ttgactggct
    10441 agcctactct tttcttcagt cttctccatc tcctctcacc tgtctctcga ccctttccct
    10501 aggatagact tggaaaaaga taaggggaga aaacaaatgc aaacgaggcc agaaagattt
    10561 tggctgggca ttccttccgc tagcttttat tgggatcccc tagtttgtga taggcctttt
    10621 agctacatct gccaatccat ctcattttca cacacacaca caccactttc cttctggtca
    10681 gtgggcacat gtccagcctc aagtttatat caccaccccc aatgcccaac acttgtatgg
    10741 ccttgggcgg gtcatccccc cccccacccc cagtatctgc aacctcaagc tagcttgggt
    10801 gcgttggttg tggataagta gctagactcc agcaaccagt aacctctgcc ctttctcctc
    10861 catgacaacc aggctcccag gtcccgaaaa ccaaagaaga agaacgcaga tcgcagatcg
    10921 cagatctcta gagtcaccga gttgctttac cggatgaaga gggccgaaac atactgtcca
    10981 aggcccttgc tggcaatcca cccaactgaa gccagacaca aacagaaaat tgtggcaccg
    11041 gtgaaacaga ctttgaattt tgaccttctc aagttggcgg gagacgtcga gtccaacccc
    11101 ggaccggtgc atgtggaccc caagaaaaag cggaaggtgg accctaaaaa gaaacgcaaa
    11161 gtggatccta agaaaaaaag aaaggtgcac gtgtccatgg tgtccaaggg cgaggaactg
    11221 ttcaccggcg tggtgcccat cctggtggaa ctggatggcg acgtgaacgg ccacaagttc
    11281 agcgtgtccg gcgagggcga aggcgacgcc acatacggaa agctgaccct gaagttcatc
    11341 tgcaccaccg gcaagctgcc cgtgccttgg cctaccctcg tgaccacact gacctacggc
    11401 gtgcagtgct tcagcagata ccccgaccat atgaagcagc acgacttctt caagagcgcc
    11461 atgcccgagg gctacgtgca ggaaagaacc atcttcttta aggacgacgg caactacaag
    11521 accagggccg aagtgaagtt cgagggcgac accctcgtga acagaatcga gctgaagggc
    11581 atcgacttca aagaggacgg caacatcctg ggccacaagc tggagtacaa ctacaacagc
    11641 cacaacgtgt acatcatggc cgacaagcag aaaaacggca tcaaagtgaa cttcaagatc
    11701 cggcacaaca tcgaggacgg ctccgtgcag ctggccgacc actaccagca gaacacccct
    11761 atcggcgacg gccctgtgct gctgcctgac aaccactacc tgagcaccca gagcgccctg
    11821 agcaaggacc ccaacgagaa gagggaccac atggtgctgc tggaattcgt gaccgccgct
    11881 ggcatcaccc tgggcatgga cgagctgtac aagcctgcag gcgagggcag aggaagtctt
    11941 ctaacatgcg gtgacgtgga ggagaatccc gggccgtcca atttactgac cgtacaccaa
    12001 aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac
    12061 atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct gtccgtttgc
    12121 cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc agaacctgaa
    12181 gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa aactatccag
    12241 caacatttgg gccagctaaa catgcttcat cgtcggtctg ggctgccacg accaagtgac
    12301 agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa
    12361 cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg ttcactcatg
    12421 gaaaatagcg accgctgcca ggatataagg aatctggcat ttctggggat tgcttataac
    12481 accctgttac gtatagccga aattgccagg atcagggtta aagatatctc acgtactgac
    12541 ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac cgcaggtgta
    12601 gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc cgtctctggt
    12661 gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg
    12721 ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga agcaactcat
    12781 cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg gtctggacac
    12841 agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat accggagatc
    12901 atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg taacctggat
    12961 agtgaaacag gggcaatggt gcgcctgctg gaagatggcg atctcgagcc atctgctgga
    13021 gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc taagaagaac
    13081 agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga tgctgagccc
    13141 cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc gatgatgggc
    13201 ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc gaagagggtg
    13261 ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg tgcctggcta
    13321 gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa gctactgttt
    13381 gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat ggtggagatc
    13441 ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca gggagaggag
    13501 tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt tctgtccagc
    13561 accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa gatcacagac
    13621 actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca ccagcggctg
    13681 gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg catggagcat
    13741 ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct ggaggcggcg
    13801 gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga ggagacggac
    13861 caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa gtattacatc
    13921 acgggggagg cagagggttt ccctgccaca gcttgatgaa gatctgagct cttaattaat
    13981 gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca
    14041 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
    14101 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    14161 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    14221 ccgtttaaac taactttaaa taattggcat tatttaaagt tacatatggc ggccgcccta
    14281 aggctcgacc tcgaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
    14341 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
    14401 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
    14461 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
    14521 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
    14581 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
    14641 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
    14701 cggggcgggc gcccgaaggt cttccggagg cccggcattc tgcacgcttc aaaagcgcac
    14761 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca tccatctaga
    14821 tctcgagcag ctgaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
    14881 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
    14941 gcgccacacc gtcgatccag atcgccacat cgagcgggtc accgagctgc aagaactctt
    15001 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
    15061 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
    15121 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
    15181 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
    15241 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
    15301 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
    15361 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
    15421 gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
    15481 cgcacgaccc catgcatcga tgatatcaga tccccgggat gcagaaattg atgatctatt
    15541 aaacaataaa gatgtccact aaaatggaag tttttcctgt catactttgt taagaagggt
    15601 gagaacagag tacctacatt ttgaatggaa ggattggagc tacgggggtg ggggtggggt
    15661 gggattagat aaatgcntgc tctttactga aggctcttta ctattgcttt atgataatgt
    15721 ttcatagttg gatatcataa tttaaacaag caaaaccaaa ttaagggcca gctcattcct
    15781 cccactcatg atctatagat ctatagatct ctcgtgggat cattgttttt ctcttgattc
    15841 ccactttgtg gttctaagta ctgtggtttc caaatgtgtc agtttcatag cctgaagaac
    15901 gagatcagca gcctctgttc cacatacact tcattctcag tattgttttg ccaagttcta
    15961 attccatcag aagctggtcg agtaacttta aataattggc attatttaaa gttaggcgcg
    16021 ccataacttc gtatagcata cattatacga agttatgtcg agatatttaa atattagacc
    16081 cagctttctt gtacaaagtg gttgatatct ctatagtcgc agtaggcgga atcatattca
    16141 gaacatctac tgacccaact gacctctaac aaatctgagc ttctaccaga gcaaatcgtt
    16201 cacatgtcaa ccagagaaca acgaacaagg atgtagtatc tagctcgaga gttctaaata
    16261 tccatcatca gtctgctcat tcatctttta attcacccat aaaggctttg aaaactaagg
    16321 ctggagatga acttatagga gcctgccagg ccgtggagag tgaggaagca gagatgacgg
    16381 agatgatgtc tttccttgtc ctgtgaaatg gattgtgggt agaggttccg gagataatgc
    16441 ctcttgctgg aaacagtctg ggcagttctg ttcccgccat tcacagaatt cttctcactt
    16501 tctaggtctt cttggtccta agggtgagac aggagatgtt ggaatgacag gagctgaagg
    16561 gccacggggc ttccccggaa cccctggcag gaaaggagag cctggagaag ccgcttatgt
    16621 gtatcgctca gcgttcagtg tggggctgga gacccgcgtc actgttccca atgtacccat
    16681 tcgctttact aagatcttct acaaccaaca gaatcattat gacggcagca ctggcaagtt
    16741 ctactgcaac attccgggac tctactactt ctcttaccac atcacggtgt acatgaaaga
    16801 tgtgaaggtg agcctcttca agaaggacaa ggccgttctc ttcacctacg accagtatca
    16861 ggaaaagaat gtggaccagg cctctggctc tgtgctcctc catctggagg tgggagacca
    16921 agtctggctc caggtgtatg gggatgggga ccacaatgga ctctatgcag ataacgtcaa
    16981 cgactctaca tttactggct ttcttctcta ccatgatacc aactgactgc aactacccat
    17041 agcccataca ccaggagaat catggaacag tcgacacact ttcagcttag tttgagagat
    17101 tgattttatt gcttagtttg agagtcctga gtattatcca cacgtgtact cacttgttca
    17161 ttaaacgact ttataaaaaa taatttgtgt tcctagtcca gaaaaaaagg cactccctgg
    17221 tctccacgac tcttacatgg tagcaataac agaatgaaaa tcacatttgg tatgggggct
    17281 tcacaatatt cgcatgactg tctggaagta gaccatgcta tttttctgct cactgtacac
    17341 aaatattgtt cacataaacc ctataatgta aatatgaaat acagtgatta ctcttctcac
    17401 aggctgagtg tatgaatgtc taaagaccca taagtattaa agtggtaggg ataaattgga
    17461 gggcagggta cagtctgttc tcattggatg gcaccatttt tcgaaagcac gtgttcattt
    17521 agagtgttgg cagagatctc attcccacct catttgtggg tctcagttta catccattaa
    17581 gacttctccc ctgtagagct ctctcagaga tggtggtcct atgtctaggt cctattctga
    17641 tattcattgg ctccctcttc acttcgcaca ttttcctttc ctgagatggc gcaacgcaat
    17701 taatgataac ttcgtatagc atacattata cgaagttatg gtctgagctc gccatcagtt
    17761 cattccctgt ccttcaaggc cacttgggta ggtgatgttc tgatgtgacc ttgcagggtt
    17821 gtgggtgtag gggaagaggg gagagggaga gagcccgcgt ccagccagag ttcctgtgct
    17881 ctgggcaggc ggatgtggga ggactgccgg acactttcca cacagccctg ggtgggcatc
    17941 tggctgtgtg aagccactga ccccacacgg ttggggggtg gacaaggggc agcccccggt
    18001 accaggttct taatctctgg catcccactc tgtatacagg agaggagctg agaacagaat
    18061 aggagctcta gggcaacact ctcagccccg aagatatggg aagagagcag agggagagat
    18121 gttcccacac aggagagagt tctaagtctg gtccatggct ggagcacagg aaggccttct
    18181 attggaaggt tagaagcagc tcgttaggaa aaagcctatc ccattgtcca agcacagcgg
    18241 gactagatga acagagacag tctatggttt tagagcttta ttgtagaaag ccagggggaa
    18301 agagagaagg tagaaagaga gagagagacc agccatggcc aagaggagag aatggggaaa
    18361 aggagagaga ggagaaaggc tagagtaaga aagcttaaag agggagaggt aaaagcttag
    18421 agagagagag agagagagag agagagagag agagtgtgag agagagagag agagggagag
    18481 agagctaagc agcccctcct atagtaggct tgttatcttg ctgttgctag gtaactggga
    18541 ggagtctagc ctgaaggtca gaagcttggg acattttgta tgtctagcta gccaccagcc
    18601 tctccggtgg gggactgtgg gggcagtaac ttcaacagga gccaggggtc caggagtcat
    18661 gagaggacag cttccgtccc atgtactggg gttccacctc ttaactcaac tagagaccaa
    18721 gctgtctgtg catagcccat tgccccacat gacctcactt caactccctc tttttaaaag
    18781 ctttattgta gaaagacagg gggaaagaga gaaggtagaa agagggagag agaccagcca
    18841 tggccaagag gagagaatgg ggaaaaggag agagaggaga aaggctagag taagaaagct
    18901 taaagaggga gagataaaag cttagagagt gagagagaga gagagagaga gagagagaga
    18961 gagagaagag agagagtttt aaaatatcca aatgtggggc tcagtggtgt ggaggctcta
    19021 ggtttaattt aacttgctgt cttcatcagt gttttttcca ctgatgaaac tctgtgtctg
    19081 cgacgtgttc cccttacatt aggggctaaa ttgcctgttc aggctgagct tccttcccct
    19141 ctgaatcctc cctgaagatc tttgacaagt acttcaaaaa caggagtctc ctgaaatcca
    19201 tttgtattca ttgtttgctt cagtttctct tcaacactca ttcttatcta acgaactatt
    19261 aggacattta gaaaccctgt ggtttaagca cccaaccccc atcctaatgg tcagcttcac
    19321 ccttcccaga gaggtgaagg atgactgtgc tttctacagt gccagctctg tattatccct
    19381 caaaaaagta atttcagtct aaaccacaaa gcagggctga actgacctca ttctttcttc
    19441 gagaactgac aagcagaagg agggtggatg atccagaatt gattcttatt tcccaagaat
    19501 tacagtagac aggctgggga gaggaatttg tgtttaagag catttgctgg ggctggagag
    19561 atggctcagt agttaagagc actgactgct cttccagagg tcctgagttc aaattccagc
    19621 aaccacatgg taactcagaa ccatctgtaa tcatctgtaa cgagatctga tgccctcttc
    19681 tggtgtgtct gaagatagct acagtgtact tacatataat aataaataaa tctttaaaaa
    19741 aaaaaaagag cacttgctgt ctttagagag gacctgggtt ctgttgccag cacccacact
    19801 ggacagctca cagctacctg taactttagt tccagggaat ctgatgctcc tttctggttt
    19861 ctgtatgcac atggtgctca taaatatacc caaggcacac acacatacac ataaaataaa
    19921 caaatcttaa aataccatac catagaagct ggttaaatac atttggccca agaccttgat
    19981 cagttgggag cctttgtttt ttgtttttgt ttttgttttt ggttttttga gacagggttt
    20041 ctctatatag ccctggctgt cctggaactc actttgtaga ccaggctggc ctcaaactca
    20101 gaaatccacc tgcctctgcc tcctgagttg ctgggattaa aggcatgtgc caccacaccc
    20161 ggctttgttt gtttgttgtt gctggttttt tttttttttt tttttttttt tttttttttt
    20221 ttttgcagtg ggatcctttg aaggatcacc ttgacacaca ctacctgact ctcagtgtgt
    20281 actaaggtga tgaggaggcc tccctggctt ccccatagtc ctatggtttg ttgttccttc
    20341 tgtgagcagt gggcatatga ctgatgcaat gatggtactt ggaattaaac ctagagcctc
    20401 cacaccactg agccctacat ttggatattt ttaaactgac cttcaggggc tggagagatg
    20461 gctcagtgtt aagagtaccc tttgctcttc cagaagtcct gagttcaatt cccagcaacc
    20521 acatggtggc tcacaaacat ctgtaatgag atctgacacc ctcttctggt gtgtctgaag
    20581 acaacggcag tgtactcaca tacataaaac aaaacaaaac aaaaccctga tcttgaaagc
    20641 caagaggtga ttggaaattg aaaggcaaaa tgattcttga aagtgagggg acttgaggga
    20701 ggggctgttt aagggggact ggggggggga cggttgagat gcaaagtgaa taaataaata
    20761 atggataaaa gcccaaaatg atgctttcaa atgatttagt ttacatcctt taataaacgt
    20821 cttttgtata aattatgaaa tagagctaca agtgccccac agtgataaac gttccttcag
    20881 gagagaactg cttaggggac tctttaaagg cttagaaacc ccgacatcta tgcaaaggtt
    20941 gctgttttca aatttcggtt ctggatgatt cccagtggta aa
//