LOCUS       112194#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20990 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 112194
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000163783
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            9034..9192
                     /db_xref="ENSEMBL:ENSMUSE00000214229"
                     /rank="6"
                     /note="ENSMUSE00000214229"
     exon            8584..8722
                     /db_xref="ENSEMBL:ENSMUSE00000214222"
                     /rank="5"
                     /note="ENSMUSE00000214222"
     exon            8359..8514
                     /db_xref="ENSEMBL:ENSMUSE00000214223"
                     /rank="4"
                     /note="ENSMUSE00000214223"
     exon            7550..7639
                     /db_xref="ENSEMBL:ENSMUSE00000227803"
                     /rank="3"
                     /note="ENSMUSE00000227803"
     exon            7249..7306
                     /db_xref="ENSEMBL:ENSMUSE00000214218"
                     /rank="2"
                     /note="ENSMUSE00000214218"
     exon            6291..6539
                     /db_xref="ENSEMBL:ENSMUSE00000902590"
                     /rank="1"
                     /note="ENSMUSE00000902590"
     misc_feature    4275..10126
                     /note="5 arm"
     misc_feature    10154..10178
                     /note="B1 Gateway"
     misc_feature    10191..10238
                     /note="Frt"
     misc_feature    10239..10738
                     /note="mouse En2 intron"
     primer          complement(10307..10328)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10203..10226)
                     /note="FRT3"
     primer          14404..14422
                     /note="seq primer NF"
     misc_feature    14464..14471
                     /note="RE sites"
     misc_feature    16264..16297
                     /note="loxP"
     misc_feature    complement(14472..14503)
                     /note="Rox"
     misc_feature    complement(16224..16255)
                     /note="Rox"
     misc_feature    14504..14525
                     /note="polylinker"
     CDS             15084..15680
                     /note="puromycin N-acetyl transferase"
     misc_feature    15199..15204
                     /note="Mutated RsrII site"
     intron          10739..11113
                     /note="mouse En2 intron"
     misc_feature    11111..11113
                     /note="Splice Acceptor"
     exon            11114..11167
                     /note="mouse En2 exon"
     primer          11014..11035
                     /note="seq primer FCHK2"
     misc_feature    11168..11290
                     /note="1Dleader"
     misc_feature    11291..11342
                     /note="F2A"
     misc_feature    11438..12154
                     /note="EGFP"
     misc_feature    11348..11437
                     /note="3X NLS"
     misc_feature    14220..14458
                     /note="SV40 polyadenylation site"
     misc_feature    13253..14197
                     /note="ERT2"
     promoter        14536..15047
                     /note="PGK promoter"
     misc_feature    15791..16223
                     /note="PGK pA"
     primer          14278..14301
                     /note="LRPCR RAF5"
     misc_feature    12164..12217
                     /note="T2A"
     misc_feature    12214..12214
                     /note="A to G to kill RsRII site"
     misc_feature    12218..13243
                     /note="Cre"
     misc_feature    11129..11168
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    11129..11144
                     /note="FCHK QC prefix region"
     misc_feature    11145..11162
                     /note="FCHK QC critical region"
     misc_feature    11163..11168
                     /note="FCHK QC suffix region"
     misc_feature    16320..16340
                     /note="B2 Gateway"
     misc_feature    10127..16370
                     /note="Synthetic Cassette"
     exon            16789..16985
                     /db_xref="ENSEMBL:ENSMUSE00000214217"
                     /type="targeted"
                     /rank="7"
                     /note="target exon 1 ENSMUSE00000214217"
     misc_feature    16371..17113
                     /note="Critical Region"
     misc_feature    17137..17170
                     /note="Downstream LoxP"
     misc_feature    17114..17193
                     /note="synthetic loxP region"
     exon            20778..20885
                     /db_xref="ENSEMBL:ENSMUSE00000214221"
                     /rank="16"
                     /note="ENSMUSE00000214221"
     exon            20390..20632
                     /db_xref="ENSEMBL:ENSMUSE00000214227"
                     /rank="15"
                     /note="ENSMUSE00000214227"
     exon            19831..19964
                     /db_xref="ENSEMBL:ENSMUSE00000214232"
                     /rank="14"
                     /note="ENSMUSE00000214232"
     exon            19031..19272
                     /db_xref="ENSEMBL:ENSMUSE00000214233"
                     /rank="13"
                     /note="ENSMUSE00000214233"
     exon            18765..18953
                     /db_xref="ENSEMBL:ENSMUSE00000214220"
                     /rank="12"
                     /note="ENSMUSE00000214220"
     exon            18614..18690
                     /db_xref="ENSEMBL:ENSMUSE00000214225"
                     /rank="11"
                     /note="ENSMUSE00000214225"
     exon            18302..18529
                     /db_xref="ENSEMBL:ENSMUSE00000214234"
                     /rank="10"
                     /note="ENSMUSE00000214234"
     exon            17847..17997
                     /db_xref="ENSEMBL:ENSMUSE00000214226"
                     /rank="9"
                     /note="ENSMUSE00000214226"
     exon            17546..17668
                     /db_xref="ENSEMBL:ENSMUSE00000214219"
                     /rank="8"
                     /note="ENSMUSE00000214219"
     misc_feature    17194..20990
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggacaggaa tcaagaattc ctaactttta aatcggatga tgatctgagt
     4321 aacatgattt aatcctgagc catccagttc tgtctcaccc aggatatgaa tcagcccttg
     4381 tcaagagtgt ccacggtgta tatgctaccc acctgttagt cacctagaag acatctcagc
     4441 tatcagatca gctctgtagt atcgcagtgc tagtcatctg acaaccttta cagggtagtg
     4501 gcatgggtaa tttatatatg ccaaagagag gctataaagg gcttcattta agtgaaaagg
     4561 catatattgg aagaaatagc atatatagac agtttagtat tatccctcca ctgggggtct
     4621 tagagtgtat tctccatgaa taaggaaggc tcctgaacat gttttcagaa gctaacatgg
     4681 ccacttgctg atgagaggaa gtggcagagt ggggactgat atatataggt ctgttcattt
     4741 ccaaagccaa gaggtagcct gaggcaaggg cccacgtgtt tcttcattcc acaaactctt
     4801 gggagccctt gagacctagt tagaaccctg ttacacctca ctcaatatca aaaagatgtt
     4861 tgaagatcgt agttcctcaa tgtgtgttca agggaacctc ggagaccccc ccacatcctc
     4921 ccccaggaag taactgctca aagcttctac tgtgtgtgtg tgtgcgcaca cacacacaca
     4981 gagagagaga gagagagaga gagagagaga gagagagaga gagggagagg gagagggaga
     5041 gggagaggga gagggagagg gagagggaga gggagaggga gagggagagg gagagggaga
     5101 gagggagaga gagagagatg aggaaagctt gctctgccat gggaaggtcc ccaaacctga
     5161 aaaagaaacc caggcgcctt gggcaaacgg cttagcctct ctgtacccca gaggggtatg
     5221 acaaggggac agttccctct tcagaatgag tgggtgggat agcagcgata tgtgctcaca
     5281 gaagttctgg gtgctggtta gtaggcactc agtatgtgtg agacctcctt caacaacccc
     5341 acatatagca ctaacctagg ctcagtgcct cagtgatcct aaaacagaca ctcagcttgt
     5401 cccagtcccg cttccatctg gaatgctgac cagatatacc atgagcagtc ctcgggggag
     5461 accccaaaac atggaaggaa agtgagagga gggagaccag ggtcttcata ctctgtaccc
     5521 tcatctgaga ttcccagaaa acaaggtttc tgctgggtct gcctttcagg ccttacaggg
     5581 ggaggtgagg aggccctggt cagccctgta actccctcaa agtcacctga gctggctcgg
     5641 tcttctcttt tctgcttaga gacatgagac aggtcagagc ctcaccctta tctgtgttca
     5701 ggagaccatg tgaggctagg agagcttcag ggggacctct aggcttctgt cccacccact
     5761 ggtctcactg atgaatgttc tagagctccg aacacacact tggggtcagc tgccctttgg
     5821 gttacactcc cagtcctttc cttagctcca cgggccactt ggggcaggac aaggtcatag
     5881 cctactcaga ggaactccga agctaatacg tgagaaacaa gctggtgtct tctgggcatc
     5941 cgtagaggca gttcatggta tggagtgggg gagcctaatc cagcctgtga atgtaagggc
     6001 tttcctgaag aaggggttat tagtgcttgt tgctaaagcg taagcaggta cttactgtcc
     6061 tgtagaaagg tgggaagagc attccaggca caaggaacaa tatctggaga gatctggcta
     6121 cagatgccac tgcacagagg agaagtggga gccaagtctg aacacacaca cacacacaca
     6181 cacacacaca cacagaagtt atgtctaagc ccagagggcc agcaagtgct cattgggctg
     6241 tgtcaagggg gcagtgacag accaagagct gcccacctcc tagggctggc agtcacggat
     6301 gctgagcaga tctggctctc caaagtcaat aagtaacttg gggggactag gcggggccag
     6361 gcctgctcct gtgggccccg gtggcatttt ccactcctga gcaagcacgg ccagaccgcc
     6421 tacctgctca agtgtccacc ttgcctcgcc ccacctaagc caaatttgcc agagctccct
     6481 gaagaaggat tcctttctcc tggaaactgg accaagggag aggctttggg catctgaagg
     6541 tgagtgcctg agagctcttg ggtgggtagg actgaggttg ggagtagggg cactgcccta
     6601 tccttcagat atctgggtaa gaatattcag ggatccatct tgcagtcgtg tgtgtgtgtg
     6661 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtt aacattgcac attcttaact
     6721 tcctgctgag atctgcaaaa gtttctgcat taggggcagt ctggtatctg gagggtggca
     6781 ctgagaaact caataggccc caaggcctga gctacaagtc ttctgttgct gaaggaatcg
     6841 tcttgtgtgg aatcctctgt gaccttggtc aggccctgcc attcaggcct tacatgaagc
     6901 acggaactct gtcctcagaa atgatagctg tctgtccagt tgctaatttc tgtgattggt
     6961 ggcctgctga ataattgtca ctttgggaat ctgggtgcta atgaggggtg gggggagatg
     7021 gcaagaagga gaaaggggtt tggaagtgaa aacctctatg gtgctgttta taagagcaag
     7081 atcgaagaag caggattgtt gggccttact tggactgact gagaagctga acatttgcaa
     7141 catgcaggga ggagactagt ccatactgag gtccctctgc ctgtatatac agggtagaac
     7201 ccttcttctt gaccttctga cctaaattct cattcccatg tccctcaggt ctgccttgac
     7261 catgatctct gcccggccgt ggctacttta cctctctgtt attcaggtga gttgggccct
     7321 gtctctttct tcagatctca aacttagggg ttagcaccca tgtccccagt cccactagac
     7381 tcctgacact ggccataaga gtagtatggg agttgtgagt tcaaagtcat aactatgggc
     7441 ctgctatgga agcaggaagc ctaagaccag ggggcggaca gactaagatc tggtgggtca
     7501 gggagaagga gtctgtgtct aaggaacacg gtaagaactt cccccacagg ctttcaccac
     7561 tgaggcccag cctgcagaaa gcctgcacac agaagtccct gaaaactatg gtggaaattt
     7621 ccctttttac atactcaagg taagccggaa tgtggggtgg cttcccatcc cccccccaat
     7681 accatgcttc tcctgccaat tggtccagtt ctcttccctg atttgaatct cctatgagtc
     7741 ttgcttccta gacagtgcat ggattagtgt aagcaactaa caagatttta gtcccagaga
     7801 agagctgagc tttgagttaa agaacagaaa cgggtggtac tgggtgtggc agcaggtgcc
     7861 gctcatccta gcactggaga ggatgaagag tttgatgcca gcctaggcca catcgtgaga
     7921 ccttgttcca ctatgttcca gttttggaag gagggtgtca gggagcatgg gggatgtcag
     7981 gtattagagg gaaatttcct cctttgaaga cctaccagat ttgagatcaa atgcaggtgc
     8041 cagctccttc tgagactcag tttccccata tttaaaatgg agtctgtagt taaaagtggt
     8101 atcccctagc ccagtggttc ttaaccttcc taaggctgca accctttcat acagttccat
     8161 gttgtggtga ccctccccca accataaaat gattttgcta cttcataact gtaatttggc
     8221 cactgttagg gatcataagg taaatagatg acatgcagga tattgatatg aaccctgttg
     8281 ggggtcacaa cccacaggtt gagaaaccac tgagtctaac cctggctcct agttcttttg
     8341 tctgttttct cccaccagct accactaccc ctggggagag atgaaggcca cattgtccta
     8401 tcaggagact caaacacggc agatcaaaac acctttgctg tggacacaga ctctggcttt
     8461 ctagtggcga caaggaccct ggaccgggaa gagaaagcag aataccaact acaggtatga
     8521 cagagtgagg cacaaggcaa gcaggcaaga cagagttggg aaccaattac cctttcctac
     8581 caggtcacct tggagtctga ggatggacgt atcttgtggg gtccacagct tgtgactgtg
     8641 catgtgaaag atgagaatga ccaggtaccc caattctccc aggccatcta cagagctcag
     8701 ctgagccagg gcaccaggcc tggtaagtga cataggcaac cgaagtgtgg tgaatacagg
     8761 caaacattga taagacatca gacagtaaag agacagttgc cgatgacaaa tctgcaagat
     8821 tctagctgtc ccccgagttt gtcccattct gtaccctcca actccgttaa tggttctgtc
     8881 aactttgggt ctcatttgtg aattgggatg aaaaacaggc tcccttcctg aaccctgata
     8941 cagtaatgag cacagaagaa gctctgagaa gagagaggta gatacagaag accttctgtc
     9001 cccaccatcc cttacaatgt atccccaccc caggggtccc cttcctcttc cttgaggctt
     9061 ctgatgggga tgcaccaggc acagctaact ccgaccttcg cttccacatt ctgagccagt
     9121 ccccacctca gcctttacca gacatgttcc agctggaccc tcacctaggg gctctggctc
     9181 ttagtcccag tggtaagcat gaactcggtt tgaaggaggg agggtcaggg aatgctggga
     9241 aaggggcctc tgagctataa gggatgggtg ggctggagct gagatagaag agaagaggaa
     9301 gttctaggca gaggccgagg ctgggttata cgtggcaagc ttagggatgg gatggatagg
     9361 gaccagatgg gaacggatga aggatctgta gttaagatgc ctcaggcatg gtatcaagtt
     9421 agatctactt ctgggagttt gatcagaggt acaagttagg gatagaagaa aaagtgactc
     9481 atacctcaac ccaaccagcc acctttacac tcctctagcc cctaaacggt ctgcacagcc
     9541 catgtgtcca acacttgctc atccgtatac tatcctatcc tatagacgcc ccacctagtc
     9601 aatcagcaag tccctcagcc caacacctac ctatctaccc tcctcccaac cctagtcatc
     9661 cagctagctc accagtcaat cactaaggaa gggaaattgc aatcaagtac tggtcgggtg
     9721 ctccaatggt gataagagag acagctctgt ggttaagagc actggctgct cttacagggg
     9781 actctgagtt tgattctcaa cacccacatg ccatttcata atccgtctgt aatccagtcc
     9841 ctgggaattg gatgtcctct gctggccctt gctggccctt gtacttacac agacacacac
     9901 agacacataa ataaataata gaatatagct ttaaaaaaaa aacaattcta tctcatccct
     9961 tagtattttt ctttcttttt atcttttcga gtctttttgc tgctgctgtt gtttttccag
    10021 acaaatattc ttgtaaccct ggctctcctg gaactagctc tgtagactag gctggcctca
    10081 aagtcataga ggccactgcc tctcaagtgc tgggatcaaa ggcatgaagg cgcataacga
    10141 taccacgata tcaacaagtt tgtacaaaaa agcaggctgg cgccggaacc gaagttccta
    10201 ttccgaagtt cctattctct agaaagtata ggaacttcga accctttccc acaccaccct
    10261 ccacacttgc cccaaacact gccaactatg taggaggaag gggttgggac taacagaaga
    10321 acccgttgtg gggaagctgt tgggagggtc actttatgtt cttgcccaag gtcagttggg
    10381 tggcctgctt ctgatgaggt ggtcccaagg tctggggtag aaggtgagag ggacaggcca
    10441 ccaaggtcag cccccccccc ctatcccata ggagccaggt ccctctcctg gacaggaaga
    10501 ctgaagggga gatgccagag actcagtgaa gcctggggta ccctattgga gtccttcaag
    10561 gaaacaaact tggcctcacc aggcctcagc cttggctcct cctgggaact ctactgccct
    10621 tgggatcccc ttgtagttgt gggttacata ggaaggggga cgggattccc cttgactggc
    10681 tagcctactc ttttcttcag tcttctccat ctcctctcac ctgtctctcg accctttccc
    10741 taggatagac ttggaaaaag ataaggggag aaaacaaatg caaacgaggc cagaaagatt
    10801 ttggctgggc attccttccg ctagctttta ttgggatccc ctagtttgtg ataggccttt
    10861 tagctacatc tgccaatcca tctcattttc acacacacac acaccacttt ccttctggtc
    10921 agtgggcaca tgtccagcct caagtttata tcaccacccc caatgcccaa cacttgtatg
    10981 gccttgggcg ggtcatcccc ccccccaccc ccagtatctg caacctcaag ctagcttggg
    11041 tgcgttggtt gtggataagt agctagactc cagcaaccag taacctctgc cctttctcct
    11101 ccatgacaac caggctccca ggtcccgaaa accaaagaag aagaacgcag atcgcagatc
    11161 gcagatctct agagtcaccg agttgcttta ccggatgaag agggccgaaa catactgtcc
    11221 aaggcccttg ctggcaatcc acccaactga agccagacac aaacagaaaa ttgtggcacc
    11281 ggtgaaacag actttgaatt ttgaccttct caagttggcg ggagacgtcg agtccaaccc
    11341 cggaccggtg catgtggacc ccaagaaaaa gcggaaggtg gaccctaaaa agaaacgcaa
    11401 agtggatcct aagaaaaaaa gaaaggtgca cgtgtccatg gtgtccaagg gcgaggaact
    11461 gttcaccggc gtggtgccca tcctggtgga actggatggc gacgtgaacg gccacaagtt
    11521 cagcgtgtcc ggcgagggcg aaggcgacgc cacatacgga aagctgaccc tgaagttcat
    11581 ctgcaccacc ggcaagctgc ccgtgccttg gcctaccctc gtgaccacac tgacctacgg
    11641 cgtgcagtgc ttcagcagat accccgacca tatgaagcag cacgacttct tcaagagcgc
    11701 catgcccgag ggctacgtgc aggaaagaac catcttcttt aaggacgacg gcaactacaa
    11761 gaccagggcc gaagtgaagt tcgagggcga caccctcgtg aacagaatcg agctgaaggg
    11821 catcgacttc aaagaggacg gcaacatcct gggccacaag ctggagtaca actacaacag
    11881 ccacaacgtg tacatcatgg ccgacaagca gaaaaacggc atcaaagtga acttcaagat
    11941 ccggcacaac atcgaggacg gctccgtgca gctggccgac cactaccagc agaacacccc
    12001 tatcggcgac ggccctgtgc tgctgcctga caaccactac ctgagcaccc agagcgccct
    12061 gagcaaggac cccaacgaga agagggacca catggtgctg ctggaattcg tgaccgccgc
    12121 tggcatcacc ctgggcatgg acgagctgta caagcctgca ggcgagggca gaggaagtct
    12181 tctaacatgc ggtgacgtgg aggagaatcc cgggccgtcc aatttactga ccgtacacca
    12241 aaatttgcct gcattaccgg tcgatgcaac gagtgatgag gttcgcaaga acctgatgga
    12301 catgttcagg gatcgccagg cgttttctga gcatacctgg aaaatgcttc tgtccgtttg
    12361 ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa tggtttcccg cagaacctga
    12421 agatgttcgc gattatcttc tatatcttca ggcgcgcggt ctggcagtaa aaactatcca
    12481 gcaacatttg ggccagctaa acatgcttca tcgtcggtct gggctgccac gaccaagtga
    12541 cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa gaaaacgttg atgccggtga
    12601 acgtgcaaaa caggctctag cgttcgaacg cactgatttc gaccaggttc gttcactcat
    12661 ggaaaatagc gaccgctgcc aggatataag gaatctggca tttctgggga ttgcttataa
    12721 caccctgtta cgtatagccg aaattgccag gatcagggtt aaagatatct cacgtactga
    12781 cggtgggaga atgttaatcc atattggcag aacgaaaacg ctggttagca ccgcaggtgt
    12841 agagaaggca cttagcctgg gggtaactaa actggtcgag cgatggattt ccgtctctgg
    12901 tgtagctgat gatccgaata actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc
    12961 gccatctgcc accagccagc tatcaactcg cgccctggaa gggatttttg aagcaactca
    13021 tcgattgatt tacggcgcta aggatgactc tggtcagaga tacctggcct ggtctggaca
    13081 cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa taccggagat
    13141 catgcaagct ggtggctgga ccaatgtaaa tattgtcatg aactatatcc gtaacctgga
    13201 tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc gatctcgagc catctgctgg
    13261 agacatgaga gctgccaacc tttggccaag cccgctcatg atcaaacgct ctaagaagaa
    13321 cagcctggcc ttgtccctga cggccgacca gatggtcagt gccttgttgg atgctgagcc
    13381 ccccatactc tattccgagt atgatcctac cagacccttc agtgaagctt cgatgatggg
    13441 cttactgacc aacctggcag acagggagct ggttcacatg atcaactggg cgaagagggt
    13501 gccaggcttt gtggatttga ccctccatga tcaggtccac cttctagaat gtgcctggct
    13561 agagatcctg atgattggtc tcgtctggcg ctccatggag cacccagtga agctactgtt
    13621 tgctcctaac ttgctcttgg acaggaacca gggaaaatgt gtagagggca tggtggagat
    13681 cttcgacatg ctgctggcta catcatctcg gttccgcatg atgaatctgc agggagagga
    13741 gtttgtgtgc ctcaaatcta ttattttgct taattctgga gtgtacacat ttctgtccag
    13801 caccctgaag tctctggaag agaaggacca tatccaccga gtcctggaca agatcacaga
    13861 cactttgatc cacctgatgg ccaaggcagg cctgaccctg cagcagcagc accagcggct
    13921 ggcccagctc ctcctcatcc tctcccacat caggcacatg agtaacaaag gcatggagca
    13981 tctgtacagc atgaagtgca agaacgtggt gcccctctat gacctgctgc tggaggcggc
    14041 ggacgcccac cgcctacatg cgcccactag ccgtggaggg gcatccgtgg aggagacgga
    14101 ccaaagccac ttggccactg cgggctctac ttcatcgcat tccttgcaaa agtattacat
    14161 cacgggggag gcagagggtt tccctgccac agcttgatga agatctgagc tcttaattaa
    14221 tgatcataat cagccatatc acatctgtag aggttttact tgctttaaaa aacctcccac
    14281 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
    14341 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    14401 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    14461 tccgtttaaa ctaactttaa ataattggca ttatttaaag ttacatatgg cggccgccct
    14521 aaggctcgac ctcgaaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
    14581 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
    14641 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
    14701 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
    14761 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
    14821 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
    14881 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
    14941 gcggggcggg cgcccgaagg tcttccggag gcccggcatt ctgcacgctt caaaagcgca
    15001 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc atccatctag
    15061 atctcgagca gctgaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
    15121 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
    15181 cgcgccacac cgtcgatcca gatcgccaca tcgagcgggt caccgagctg caagaactct
    15241 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
    15301 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
    15361 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
    15421 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
    15481 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
    15541 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
    15601 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
    15661 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
    15721 gcgcacgacc ccatgcatcg atgatatcag atccccggga tgcagaaatt gatgatctat
    15781 taaacaataa agatgtccac taaaatggaa gtttttcctg tcatactttg ttaagaaggg
    15841 tgagaacaga gtacctacat tttgaatgga aggattggag ctacgggggt gggggtgggg
    15901 tgggattaga taaatgcntg ctctttactg aaggctcttt actattgctt tatgataatg
    15961 tttcatagtt ggatatcata atttaaacaa gcaaaaccaa attaagggcc agctcattcc
    16021 tcccactcat gatctataga tctatagatc tctcgtggga tcattgtttt tctcttgatt
    16081 cccactttgt ggttctaagt actgtggttt ccaaatgtgt cagtttcata gcctgaagaa
    16141 cgagatcagc agcctctgtt ccacatacac ttcattctca gtattgtttt gccaagttct
    16201 aattccatca gaagctggtc gagtaacttt aaataattgg cattatttaa agttaggcgc
    16261 gccataactt cgtatagcat acattatacg aagttatgtc gagatattta aatattagac
    16321 ccagctttct tgtacaaagt ggttgatatc tctatagtcg cagtaggcgg aaaatattgt
    16381 gtatatatct atagagagag atagaaagag aggagcatgt agatatgtag atatatatta
    16441 tatgtgtatt ttgtctgtgt gtgtgtgtgg tgtgtgtgtg tgtggtgtgt gtgtgtgtgt
    16501 gtgtgtgtgt gtgtgtggag tcacatgcat gcctgatgcc agtagagggc atcaggtccc
    16561 ttggaactgt acagacaggt gttagctgcc atgtggtgct gagaatataa cgtgggtccc
    16621 ctggaagagc agtcaatgct catagccact gaaacatctc tccagttctc ttccctcaaa
    16681 gtcttttaaa cacctgtaat atgcttggct ttctgtctgg tactggctga agaggctttg
    16741 ggccttgctc cctggatcta tgctcaactg accttctatc ccttctagga agcaccagcc
    16801 tagaccatgc ccttgaagag acttaccagc tattggtaca ggtcaaggac atgggtgacc
    16861 agccttcagg ccaccaggct attgcaactg tagagatctc catagtagag aacagctggg
    16921 cacccctaga gcctgttcac ctggcagaga atctcaaagt tgtgtaccca cacagcattg
    16981 cccaggtgag aagtaactga agtaacctgc agcttgtaca gctggacctg agtcacaaca
    17041 gacacttggg aggaatgtcc aggactcaac ttgaccccta ttcctttctt catgtcccac
    17101 tacaaagtgg gcagagatgg cgcaacgcaa ttaatgataa cttcgtatag catacattat
    17161 acgaagttat ggtctgagct cgccatcagt tcactctgct agtgtgagtt tgtgctctaa
    17221 catccccagc ttgcctaagg cttcctcggg ggaaacagct gccaactttc actgccattg
    17281 gggattgtca ccaaagccag aaatgtctct ggccttttct agcaatgcct tggggtcagt
    17341 cccagaaagc agaaaataaa ataacttggt cacttaggca ctttggcaag gtccaggctt
    17401 ggaccagagt aaggttgtca taagtcgcaa accacatgct cttaggttcc aggtttcttt
    17461 cctgggaagc tgttggggaa taagggctgg catagatcct tcctgcctct cagcctaaag
    17521 ggctatcaat ttacctcctc cccaggtgca ctggagtgga ggagacgtgc actaccagct
    17581 ggagagccag cctccaggac ccttcgatgt ggatacagag gggatgctcc atgttaccat
    17641 ggagctggac cgggaggccc aggctgaggt gaggtgagag ggaaacccag ggcagactgg
    17701 agggtgctct gacacatgtc ccacagatgc ggaaacacgg aagacatccc agaagagata
    17761 gggctggcca ccagaaggga cttgaatgct ctccaggctg gagtcaccct tggccctaat
    17821 gtgaagctcc ctactgccca ctacagtacc agctccaagt ccgagctcag aattcccatg
    17881 gtgaggacta cgcagaaccc ctggagttgc aggtggtggt gatggatgaa aacgacaatg
    17941 cacctgtctg ctccccacat gacccaacag tcaacatccc tgagctcagc cccccaggtg
    18001 ggttatgggt cttgtcagga gccgggaaag gcagagctgc tagaaaccac ccatcttgat
    18061 tctttgggat aggtctagct tccgaattct atgccaaagc caaataccac acacacacac
    18121 acacacacac acacacacac acacacacac acacaccaca caccaaacac acacaaacac
    18181 attcacatac acacacacca caccacacca cacaccaaac acacacacaa atacacacac
    18241 atacacacat accatatgtg cacacacata ccaggaaatg ggtggcaatg ttgtcttaca
    18301 ggaactgaaa tagccaggct ctcagcagag gatttggatg cccctgggtc acccaattcc
    18361 cacattgtat atcagttgtt gagccctgag cctgaggagg gggctgaaaa caaagccttc
    18421 gagttagatc cgacctcagg cagtgtaaca ctgggaactg ccccactcca tgctggccag
    18481 agtatcctgc ttcaggtgct ggctgttgac ctagcaggat cagagagtgg taagtaccct
    18541 gatagtgggt gaccttggcc cttgcccctc tgctcctact gagacctcac taacaatccc
    18601 tcccctctaa caggcctcag cagcacatgt gaggtgacag tcatggtgac agacgtcaac
    18661 aaccatgccc ctgagttcat caattcccag gtaagctgac accgaggtac agagatcatg
    18721 ggaaagatgc tggtggtgtt taccaagcat ctccatcttt ccagattggg cctgtaactc
    18781 ttcctgagga tgtaaaacct ggggctctgg tggcaacact catggccact gatgctgacc
    18841 ttgaacctgc cttccgcctt atggactttg ccattgaaga aggagaccca gaagggatct
    18901 ttgacctgtc ctgggagcca gactccgacc atgtccagct cagactccgg aaggttagga
    18961 tgtgaatagg tggggaaggg tcacaagtat accgtgaccc acacagtgac cccgaactca
    19021 ctggtttcag aacctcagct atgaggcagc tcctgatcac aaggtggtgg tggtcgtgag
    19081 taacatagaa gaactggtgg gcccaggccc aggccctgca gccacagcca cagtgactat
    19141 actagtggag agggtggttg ctcccctcaa gttggaccag gagagctatg agaccagcat
    19201 cccagtcagc accccagctg gctccctcct gctgaccatc cagccctcag accccatgag
    19261 cagaaccctc aggtgagcca aaagccctgc cacttgccta gcctggactt cccatgattc
    19321 ccaaggatga cttccacacc ccacacccca cagagaaact catttcgtat gtggccaaaa
    19381 gtattcctca aatgattact aatgtcaggg ttctgactcc tggagggcca gcactgaatg
    19441 gactaggcag acatggagga ggtgtgtcta gagcccacag gagctctact tagtcctcct
    19501 taagggagca ccaccatcca gactgaaact gtacctgagt ctttgccctc cagacttctt
    19561 ggctggatgc tctggaacta gtgaagttga accagttgcc accctccatc cataggttct
    19621 gataaccctc ccccctccta cagttcaaac accatatcac caacaaattc taaaactggg
    19681 tggctgggct caccagcact ggtgttgtcc ctgagtcaca gaaaggatac aggacttgag
    19741 tatcagctgc accatcctgg gggtttctga gcagcaccct gtatattgtt ccagtgcctc
    19801 cccaaacaca ccttcatctt tgggcctcag gttctccctg gtcaatgact cagagggctg
    19861 gctctgtatc aaggaggtgt ctggggaggt acacacagcc cagtccctgc agggtgccca
    19921 gcctggagac acatacacag tgcttgtgga ggcccaagac acaggtatga tcacacagca
    19981 atggagtctt ctcgggggac ggcaacttca ctgcaaacct ttacatgaaa accatccttc
    20041 aagttgacac tcacaattcc ccaactctgt cccttgggct tctcgggcta tcaactgcag
    20101 agacagacaa gacaatgggc attagattag taaagcagaa gaagagccag gagctgtgtc
    20161 tgaggcagga atgctaagag atgggccaag tggaacaccg gagccaggtg agggggaccg
    20221 tgggcacctc aataggataa acagctagcc ctgggaaggc aagtgaggaa gggtacatca
    20281 gggtgcagga gtatgagggg aagcagtaga gtaagacaga tgggctgtgt agggcgtgtg
    20341 gagaccagcc caggttctct gaggcttacc ctcacctccc ataatacaga taagccagga
    20401 ctgagcactt ctgccactgt tgtgatccac ttcctgaagg cctctcctgt cccagcattg
    20461 actctgtctg ctgggcccag ccgacacctc tgtacacccc gccaagacta cggtgtagtt
    20521 gtgagtgggg tcagtgagga ccctgaccta gccaacagga atggtcccta cagctttgct
    20581 ctcggtccca atcccactgt gcagcgggat tggcgcctcc agcctctcaa cggtacgtgg
    20641 cagggggtgg aaatgaggct gtgaagggtg gggtgagttc acttggatgt gagatggggt
    20701 agggttggaa gtaggggagc ggcttgggtg gagcctgttc aggctagtga ccctaatcat
    20761 cttctgcaac ccaacagatt cccacgccta cctcaccttg gcattgcatt gggtagagcc
    20821 tggtgaatac atggtacctg tggttgtcca ccatgatacc catatgtggc aactccaggt
    20881 caaaggtgag gttggggctt gggtgttgcc aaagttggca tctttaattt ctacgggagc
    20941 catagggata tggggaaaag agcctgggta tagacaatgc actctcatgt
//