LOCUS       255130#pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21810 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 255130
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000073252
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8793..8898
                     /db_xref="ENSEMBL:ENSMUSE00000157316"
                     /rank="1"
                     /note="ENSMUSE00000157316"
     exon            9131..9285
                     /db_xref="ENSEMBL:ENSMUSE00000508945"
                     /rank="2"
                     /note="ENSMUSE00000508945"
     exon            9676..9720
                     /db_xref="ENSEMBL:ENSMUSE00000327098"
                     /rank="3"
                     /note="ENSMUSE00000327098"
     misc_feature    4275..10099
                     /note="5 arm"
     misc_feature    10127..10151
                     /note="B1 Gateway"
     misc_feature    10164..10211
                     /note="Frt"
     misc_feature    10212..10711
                     /note="mouse En2 intron"
     primer          complement(10280..10301)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10176..10199)
                     /note="FRT3"
     primer          14363..14381
                     /note="seq primer NF"
     misc_feature    14423..14430
                     /note="RE sites"
     misc_feature    16223..16256
                     /note="loxP"
     misc_feature    complement(14431..14462)
                     /note="Rox"
     misc_feature    complement(16183..16214)
                     /note="Rox"
     misc_feature    14463..14484
                     /note="polylinker"
     CDS             15043..15639
                     /note="puromycin N-acetyl transferase"
     misc_feature    15158..15163
                     /note="Mutated RsrII site"
     intron          10712..11086
                     /note="mouse En2 intron"
     misc_feature    11084..11086
                     /note="Splice Acceptor"
     exon            11087..11126
                     /note="mouse En2 exon"
     primer          10987..11008
                     /note="seq primer FCHK2"
     misc_feature    11127..11249
                     /note="1Dleader"
     misc_feature    11250..11301
                     /note="F2A"
     misc_feature    11397..12113
                     /note="EGFP"
     misc_feature    11307..11396
                     /note="3X NLS"
     misc_feature    14179..14417
                     /note="SV40 polyadenylation site"
     misc_feature    13212..14156
                     /note="ERT2"
     promoter        14495..15006
                     /note="PGK promoter"
     misc_feature    15750..16182
                     /note="PGK pA"
     primer          14237..14260
                     /note="LRPCR RAF5"
     misc_feature    12123..12176
                     /note="T2A"
     misc_feature    12173..12173
                     /note=" A to G to kill RsRII site"
     misc_feature    12177..13202
                     /note="Cre"
     misc_feature    11102..11127
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    11102..11117
                     /note="FCHK QC prefix region"
     misc_feature    11118..11121
                     /note="FCHK QC critical region"
     misc_feature    11122..11127
                     /note="FCHK QC suffix region"
     misc_feature    16279..16299
                     /note="B2 Gateway"
     misc_feature    10100..16329
                     /note="Synthetic Cassette"
     exon            16589..16698
                     /db_xref="ENSEMBL:ENSMUSE00000157311"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000157311"
     exon            16861..17016
                     /db_xref="ENSEMBL:ENSMUSE00000157318"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 2 ENSMUSE00000157318"
     exon            17221..17330
                     /db_xref="ENSEMBL:ENSMUSE00000157312"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 3 ENSMUSE00000157312"
     misc_feature    16330..17489
                     /note="Critical Region"
     misc_feature    17513..17546
                     /note="Downstream LoxP"
     misc_feature    17490..17569
                     /note="synthetic loxP region"
     exon            19186..19386
                     /db_xref="ENSEMBL:ENSMUSE00000157322"
                     /rank="7"
                     /note="ENSMUSE00000157322"
     exon            19871..19982
                     /db_xref="ENSEMBL:ENSMUSE00000157327"
                     /rank="8"
                     /note="ENSMUSE00000157327"
     exon            20069..20183
                     /db_xref="ENSEMBL:ENSMUSE00000327071"
                     /rank="9"
                     /note="ENSMUSE00000327071"
     exon            21669..21810
                     /db_xref="ENSEMBL:ENSMUSE00000327069"
                     /rank="10"
                     /note="ENSMUSE00000327069 5' fragment"
     misc_feature    17570..21810
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaagctcc tacctaccgc taaatcttcc taaataaaat ggaaacaagt
     4321 agtaatcaca gacgcagcca gagccaatcc ttccctacca ggtactaggc agatataatt
     4381 cgatcgattg tgaatgcatt gctctctgtt ggctctggtc ccttagggtg actccggggg
     4441 ccctttaacc tgttccgagc ccggcccgcg ccccagagag gtcctctttg gagtcacttc
     4501 ttggggagat ggctgtgggg aaccagggaa gcctggggtc tacacccgtg tgaccgtgtt
     4561 caaagactgg ctgcaggagc agatgagcgg tgagcatccc ctagctaatg gtacctcgga
     4621 taggtctggg caagccggtt tccatcccgc tactcgggtc tctgtggagg agctgcggat
     4681 gagtgacttg tagatccaca tacaaggggt gatctggcat ctgcttgcag cgggcccctc
     4741 cactcgcgag cctagctgca gagaacttct aaattggaac gctcgggaag aggagccgtt
     4801 cacggacgcc ccgggcctct gtgccttcta tgcgcgccag tgcctagggt ccgagagctc
     4861 ctgtgcgcgc ctagcgctcc aacagtgtct gcagcgcagg cgacggtgcg gtcagtcctg
     4921 tttacccctg ccagggtggg ggaggaggag taggggtggg aggttggagg gtgggggtgg
     4981 gagatggggt gcgagcccca ccattcggtg ggccccttac acctctaaac cgctcgcaga
     5041 gctacggtca ctggcgcaca ccctgcttgg tctgctgcgg ggcgcccagg agctgctcgg
     5101 cccccggcca ggattgcggc gtggagtctc cgcgcccgct cgctccgcgc cgtcgctcca
     5161 ggagcttccc ggtcacaatc cccgagaaca gcggttatac tcaggtatcc tgagtgcccc
     5221 cagccaggcc taggccccag agtctcggaa tcatgtaacc tctccgaggc gtgcccacta
     5281 acttcctgtg gtctcctcca ggatcgcgaa ttgcgggaac ctggctccag aaaccaaagc
     5341 cggagcggcg cccggaaaca aagggtaatg tcgcccccta ccggcctgct ggagaggagc
     5401 ggccaagagg ctgtttctag agagaagctg ggattgtctg ctagaaaatt ccaaatgaca
     5461 acagagaaaa ccggcgctca ctccctctct tccaggaaaa cagattcctt tttagggaga
     5521 catgtggcac taagtccttg atggtgtcag aggcacacag ccttgcatga tcacgaaacc
     5581 tctgccagcc tcagtttccc catctagagc acacagttct ctcatagtct ggggatctcc
     5641 ccttcttgca ggctgccctg ggctggagcc tctgcaacag aagttagcgg ctattcagcg
     5701 agcgcatgct tggatcctac agatcccagc tgagcacttg gccatgaact ttcatgaggt
     5761 aggtccccag gctgcccagg ctccttcaca gtggcctgtg aaagctgacc tgactggagc
     5821 tcagtagaag gaagagacag ggggatccag cctttccggg gcggagtggg ggtgagggtg
     5881 agctggaata acaggtggct atgaagatgg gaggtagaga ggtaagagtg gaagggctca
     5941 ttagagcatt cgcctgatct ggaaattaga tggatttctg ggagatgaag gggcagggca
     6001 aggctctaag tagcttccaa agctccagga ggcaagagca accctcaggt cagaaaccct
     6061 caggagtggt tttttgggtc ctaacctcct ccctctgcag gtgctggcag atctgggctc
     6121 caagacactg acaggactct tcagagcttg ggtgagggcc ggtttggggg accagcgtgt
     6181 ggtcttcagt ggcctagtgg gcttggaacc ttccacactg gctcatagtc tcccccggct
     6241 actagtgcag gccctgaagg ccttccgctc agcttccctg acagaaggag agccccaggc
     6301 accctggata ggtgctgatc aaggacagag gctgggaaag gagcgccagg gccaactcca
     6361 gcctcctgtt ccctgaacca ggcaaccctg tgatggtcac actgtcctgg gggctaaagc
     6421 ggttgtgtga gacatacaaa gtctctctat tccagttgat cttggatgtg tggatagagt
     6481 ggggatcctg gatctcactc atcttcttca atgtgtcacc agacacgcat ggctgcttta
     6541 ataaatgtta tttatactct acataaaatc tggagctttc ttgggatggg aaggggctgg
     6601 cattagatgg gagtagggca ggggccaacc tcgaagagca gttaacagag accacaaagg
     6661 agctggaagg agccagaagt ggtggcttgt gctcgccgtg ctagcattct ggagactgaa
     6721 gcaggattgc actgagtttg aatgtgagct acatagtaag ctctaggcta gcctgagcta
     6781 caaaataaga gcacccctta ccccccacac acacacacac acaaagatac gaaaccaggg
     6841 cctggaagag atagctcagt gggtaaagtt cttgatgcat atggatctga gttcaagacc
     6901 tcagaaccca ggtaaaagcc acaggggcag agtacacctg taaaccacag ccctagctaa
     6961 ggagcatggc agtgaggagc agacagacct cgggcgttca ctggccactc tagctgctcc
     7021 aggcgaggtc caggttcagc gaagaggccc tgtcttgaaa aataaggtgg aggagatcag
     7081 ctggatttca gcagcagcag cagctccaga gcctcagtcc ttctgtggct ttccgtgtcc
     7141 ccctctccct ttcaaaaccg tagcttctgg tatggctacc tcaaggtgat cggtgacgtg
     7201 caagtgattt agtacgcaag tcaaccgcgc caaggggagc aaagagagaa gctgtgcttc
     7261 tctgcctgag tgaggacccg gacaccatcc tggagcagag cagaaagatt cagcaggcga
     7321 catggcccaa acctatgaca agaccctgcc agaccaggct gccactctgc cctcaatgat
     7381 gcaacctatg agaccaatga cagcaagaag gatgccctga ggttcatctc ttgggtctct
     7441 aagagagccc ctgcaccctc acatatgagc aaaatgatca atgtctgctc caaagacacc
     7501 atcaggaagc tgacaaggtc cagcatgaag tacaaagagc tcagccacca ctgcaccttg
     7561 gcagacaatg gtgacagtat tgtcctctcc ctggaagcaa gcaagccttt atgaggccac
     7621 ctccaaactt ctgcttagaa caaagaatag gctcagactt gccagtgcat gctgcagtcg
     7681 gctcctctct tgtcagaccc tatgggcatc ccttcacccc attgctagtc tccccgacat
     7741 ctagatggcc tcctccctcc atgcctgacg gttctggctt tctgattcct attagataga
     7801 agcagatcac ggtgcccagg cacctttgag ggttgtattc tcttaagcat actcttagtg
     7861 ccatctatcc cgaccactga cttctagcca cagtcattac aacggacagg cctgactctg
     7921 tgtggaaacg accctccttg ccccagtgga tccccttttc tttccctttg gttttggcta
     7981 ctcatagatg caggaacggt gaggagactt cagttcaaat taaagaaaga aaagaaatgg
     8041 gccagtaagg tggcttagca aataaaggta cctgctgccg agccatgtga aaggaaaaaa
     8101 aaatttaact ccttcaagtt tcctctgact tccacacatg ccacaaactg cgcagaaaca
     8161 cactgagtgg atgggtggat ggatggatgg atggatggat tttatttatt tacttattta
     8221 tttatttata aatctttggg gttttttgga gacatggctt ctctgtgtaa caacctggca
     8281 gtcctggaac tcattctgta gaccaggctg gccttgaact cagagatcct tcctctcttc
     8341 ccaagtgctg ggattaaaag catacactgc cacacccagc ttagaggtaa tatttttaaa
     8401 gatgggggaa aaaatacaca agtacaggtg gagcactagc aagatggctc aacaggtaac
     8461 agttgcctgc tgtacaagaa gtttcacctc agaaccctct gtagaaagag agaagcaatt
     8521 gtgacaggtg tacacacaca cacacacaca cacacacaca cacacacaca gaggattcgc
     8581 cccccaccct ctgccaccct accccaccag gccccaccct accaacaatc tgtgacaatc
     8641 cctgcctggg atcttttcgt tctgcccttg gctcctgccc taactggcaa accccacccc
     8701 ctcatcacca gctttcaagt atcagattgc gtttccggcc tcttctttcc aaacccctaa
     8761 accaccagca cctgtcccct tgcttgcctc attccacagc caacaggctg aagggaagac
     8821 aaaccctagt cagtcagagg tgggggatgg cagggcctgt gctcacactg gggctgctgg
     8881 ctgcccttgt tgtgtgtggt aaggatgaca taaccccctt cctgggggcc ttctatgccc
     8941 acctgggcca ccgcctccct aacattatac ctaattccca ctcccactct gccctttcta
     9001 accctaggca tcccaccctc agggagcagc tggctttcct ctgctctgcc cagcccaggg
     9061 ctgccccagc aggagcacag ggaaggctcc ttcctaggtc caaacaccag caccctaccc
     9121 ctctccccag ccctcccagg cagttggggc ctgaatgagg aacaaaggct gatccagcac
     9181 ctattcaatg agaagggcta cgacaaagac ctgcggccgg tggctcgaaa ggaggacaaa
     9241 gtggatgtcg ccctaagtct caccctctcc aacctcatct ccctggtgag tagaccttcg
     9301 tgggatagcc tgagctgggg agaggatggc aagaagggac aattgctcaa aggctcctga
     9361 ccagctagct cctcttctgg gagacatgca tgactattct ttgtgccaga tggtgccacg
     9421 ggcctttagc cacccaaagc catcagctct gcctgccact gtcaagctat ccctgtgctc
     9481 ccccgggact cacatcagca gcctctgccc ttcgcttccg tagtctgctg atccacctcg
     9541 atctgttgct ctagaagacc ctggcccttc cagctgccgt ggtccctggg aaccaggcag
     9601 attagattct gctgactgag tttgggtgaa aggtagatgg gaacctttgg ctacaaccct
     9661 gttgtttgat tacagaaaga agtggaggag accctcacca ccaacgtgtg gatagatcat
     9721 gtaaggacac cctgccgaag gttccaggtc ccctggtgct cacttatgct ggggaccaca
     9781 agctcctatg gacacacctt tgggggagct tgactacaga gaacccaact gcccttttcc
     9841 actgaggcct ctactagctc cgaggacatg tcctagaagg ctggggttcc tccgtgctaa
     9901 aattgccgct gtgtcccgat ttgctttgtt gttgttttga gatggggttc cctagccctg
     9961 tttccctggc tgacctagaa cttatgacgt agaccatgct ggccttgaac tcacagagat
    10021 tcatttgcat ctgcctccca agccctggga ctaaaggtgg gattaaagca cagagtcacc
    10081 acctctggtg tgcattggaa aggcgcataa cgataccacg atatcaacaa gtttgtacaa
    10141 aaaagcaggc tggcgccgga accgaagttc ctattccgaa gttcctattc tctagaaagt
    10201 ataggaactt cgaacccttt cccacaccac cctccacact tgccccaaac actgccaact
    10261 atgtaggagg aaggggttgg gactaacaga agaacccgtt gtggggaagc tgttgggagg
    10321 gtcactttat gttcttgccc aaggtcagtt gggtggcctg cttctgatga ggtggtccca
    10381 aggtctgggg tagaaggtga gagggacagg ccaccaaggt cagccccccc cccctatccc
    10441 ataggagcca ggtccctctc ctggacagga agactgaagg ggagatgcca gagactcagt
    10501 gaagcctggg gtaccctatt ggagtccttc aaggaaacaa acttggcctc accaggcctc
    10561 agccttggct cctcctggga actctactgc ccttgggatc cccttgtagt tgtgggttac
    10621 ataggaaggg ggacgggatt ccccttgact ggctagccta ctcttttctt cagtcttctc
    10681 catctcctct cacctgtctc tcgacccttt ccctaggata gacttggaaa aagataaggg
    10741 gagaaaacaa atgcaaacga ggccagaaag attttggctg ggcattcctt ccgctagctt
    10801 ttattgggat cccctagttt gtgataggcc ttttagctac atctgccaat ccatctcatt
    10861 ttcacacaca cacacaccac tttccttctg gtcagtgggc acatgtccag cctcaagttt
    10921 atatcaccac ccccaatgcc caacacttgt atggccttgg gcgggtcatc ccccccccca
    10981 cccccagtat ctgcaacctc aagctagctt gggtgcgttg gttgtggata agtagctaga
    11041 ctccagcaac cagtaacctc tgccctttct cctccatgac aaccaggctc ccaggtcccg
    11101 aaaaccaaag aagaagaacg cagatctcta gagtcaccga gttgctttac cggatgaaga
    11161 gggccgaaac atactgtcca aggcccttgc tggcaatcca cccaactgaa gccagacaca
    11221 aacagaaaat tgtggcaccg gtgaaacaga ctttgaattt tgaccttctc aagttggcgg
    11281 gagacgtcga gtccaacccc ggaccggtgc atgtggaccc caagaaaaag cggaaggtgg
    11341 accctaaaaa gaaacgcaaa gtggatccta agaaaaaaag aaaggtgcac gtgtccatgg
    11401 tgtccaaggg cgaggaactg ttcaccggcg tggtgcccat cctggtggaa ctggatggcg
    11461 acgtgaacgg ccacaagttc agcgtgtccg gcgagggcga aggcgacgcc acatacggaa
    11521 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccttgg cctaccctcg
    11581 tgaccacact gacctacggc gtgcagtgct tcagcagata ccccgaccat atgaagcagc
    11641 acgacttctt caagagcgcc atgcccgagg gctacgtgca ggaaagaacc atcttcttta
    11701 aggacgacgg caactacaag accagggccg aagtgaagtt cgagggcgac accctcgtga
    11761 acagaatcga gctgaagggc atcgacttca aagaggacgg caacatcctg ggccacaagc
    11821 tggagtacaa ctacaacagc cacaacgtgt acatcatggc cgacaagcag aaaaacggca
    11881 tcaaagtgaa cttcaagatc cggcacaaca tcgaggacgg ctccgtgcag ctggccgacc
    11941 actaccagca gaacacccct atcggcgacg gccctgtgct gctgcctgac aaccactacc
    12001 tgagcaccca gagcgccctg agcaaggacc ccaacgagaa gagggaccac atggtgctgc
    12061 tggaattcgt gaccgccgct ggcatcaccc tgggcatgga cgagctgtac aagcctgcag
    12121 gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc gggccgtcca
    12181 atttactgac cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg
    12241 ttcgcaagaa cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga
    12301 aaatgcttct gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat
    12361 ggtttcccgc agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc
    12421 tggcagtaaa aactatccag caacatttgg gccagctaaa catgcttcat cgtcggtctg
    12481 ggctgccacg accaagtgac agcaatgctg tttcactggt tatgcggcgg atccgaaaag
    12541 aaaacgttga tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg
    12601 accaggttcg ttcactcatg gaaaatagcg accgctgcca ggatataagg aatctggcat
    12661 ttctggggat tgcttataac accctgttac gtatagccga aattgccagg atcagggtta
    12721 aagatatctc acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc
    12781 tggttagcac cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc
    12841 gatggatttc cgtctctggt gtagctgatg atccgaataa ctacctgttt tgccgggtca
    12901 gaaaaaatgg tgttgccgcg ccatctgcca ccagccagct atcaactcgc gccctggaag
    12961 ggatttttga agcaactcat cgattgattt acggcgctaa ggatgactct ggtcagagat
    13021 acctggcctg gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg
    13081 gagtttcaat accggagatc atgcaagctg gtggctggac caatgtaaat attgtcatga
    13141 actatatccg taacctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg
    13201 atctcgagcc atctgctgga gacatgagag ctgccaacct ttggccaagc ccgctcatga
    13261 tcaaacgctc taagaagaac agcctggcct tgtccctgac ggccgaccag atggtcagtg
    13321 ccttgttgga tgctgagccc cccatactct attccgagta tgatcctacc agacccttca
    13381 gtgaagcttc gatgatgggc ttactgacca acctggcaga cagggagctg gttcacatga
    13441 tcaactgggc gaagagggtg ccaggctttg tggatttgac cctccatgat caggtccacc
    13501 ttctagaatg tgcctggcta gagatcctga tgattggtct cgtctggcgc tccatggagc
    13561 acccagtgaa gctactgttt gctcctaact tgctcttgga caggaaccag ggaaaatgtg
    13621 tagagggcat ggtggagatc ttcgacatgc tgctggctac atcatctcgg ttccgcatga
    13681 tgaatctgca gggagaggag tttgtgtgcc tcaaatctat tattttgctt aattctggag
    13741 tgtacacatt tctgtccagc accctgaagt ctctggaaga gaaggaccat atccaccgag
    13801 tcctggacaa gatcacagac actttgatcc acctgatggc caaggcaggc ctgaccctgc
    13861 agcagcagca ccagcggctg gcccagctcc tcctcatcct ctcccacatc aggcacatga
    13921 gtaacaaagg catggagcat ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg
    13981 acctgctgct ggaggcggcg gacgcccacc gcctacatgc gcccactagc cgtggagggg
    14041 catccgtgga ggagacggac caaagccact tggccactgc gggctctact tcatcgcatt
    14101 ccttgcaaaa gtattacatc acgggggagg cagagggttt ccctgccaca gcttgatgaa
    14161 gatctgagct cttaattaat gatcataatc agccatatca catctgtaga ggttttactt
    14221 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
    14281 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    14341 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    14401 gtatcttatc atgtctggat ccgtttaaac taactttaaa taattggcat tatttaaagt
    14461 tacatatggc ggccgcccta aggctcgacc tcgaaattct accgggtagg ggaggcgctt
    14521 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
    14581 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
    14641 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
    14701 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
    14761 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
    14821 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
    14881 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cttccggagg cccggcattc
    14941 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
    15001 tcgacctgca tccatctaga tctcgagcag ctgaagctta ccatgaccga gtacaagccc
    15061 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
    15121 ttcgccgact accccgccac gcgccacacc gtcgatccag atcgccacat cgagcgggtc
    15181 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
    15241 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
    15301 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
    15361 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
    15421 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
    15481 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
    15541 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
    15601 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
    15661 cagcgcccga ccgaaaggag cgcacgaccc catgcatcga tgatatcaga tccccgggat
    15721 gcagaaattg atgatctatt aaacaataaa gatgtccact aaaatggaag tttttcctgt
    15781 catactttgt taagaagggt gagaacagag tacctacatt ttgaatggaa ggattggagc
    15841 tacgggggtg ggggtggggt gggattagat aaatgcntgc tctttactga aggctcttta
    15901 ctattgcttt atgataatgt ttcatagttg gatatcataa tttaaacaag caaaaccaaa
    15961 ttaagggcca gctcattcct cccactcatg atctatagat ctatagatct ctcgtgggat
    16021 cattgttttt ctcttgattc ccactttgtg gttctaagta ctgtggtttc caaatgtgtc
    16081 agtttcatag cctgaagaac gagatcagca gcctctgttc cacatacact tcattctcag
    16141 tattgttttg ccaagttcta attccatcag aagctggtcg agtaacttta aataattggc
    16201 attatttaaa gttaggcgcg ccataacttc gtatagcata cattatacga agttatgtcg
    16261 agatatttaa atattagacc cagctttctt gtacaaagtg gttgatatct ctatagtcgc
    16321 agtaggcgga aatcaaaata tcagaactga tagaggttca cggcatagaa acaagccagt
    16381 ctggcgtgga aattgtgccc cttagtggta taatgtatgt cctacacaac tgtctgtgac
    16441 gggcctgagt ctagttcttt atccatctcc tccaaatttc ttttgtccca actctcaggc
    16501 tttgtttaga cttccctggg gaagtgggga ccgctgggag ggtggttagt ggccgaatcc
    16561 acttctgatg tcctttttgg acctctaggc ctgggtagac agccgtctac agtgggatgc
    16621 caacgacttc gggaacatca ctgtcctgcg cctgccccct gacatggtgt ggctaccaga
    16681 gattgtactg gagaacaagt tgagaggaac cctcccagcc cccctctgcc tcattccctt
    16741 ccttatgctc cccagcctgc gcctccagcc ctgcctgccc accaccctct cctgcctcct
    16801 gcggtcctgc cggttcctgg ccagccagga cacacagtga ttatggtctc catcccacag
    16861 caatgatggc tcgttccaga tttcttatgc ctgcaatgtg cttgtctatg actcgggcta
    16921 tgtgacctgg ctgccgcccg ccatcttccg ctcctcctgc cccatctcag tcacctactt
    16981 ccctttcgac tggcagaact gctccctcaa attcaggttt gtccacctga atgtctctcg
    17041 ggccagggca cccagccagg gactggaagc tgtgaactaa atgctctcag acagaggctt
    17101 tggtcccatg ctgagcagaa acccctcaga tgatagcctt ccccaactag ccctccccac
    17161 tctaccacct cctaccctca gaagccctgt tcagagactt atgaccactg ctctctccag
    17221 ttcactcaag tatacagcca aggagatcac acttagcctg aagcaggagg aggaaaacaa
    17281 ccgctcttac cccatcgagt ggatcatcat tgaccctgaa ggtttcacag gtacttcaaa
    17341 agtcatggtg gatgggcccc aaagatgcag acatctgtgg aagttcactt acacctcaca
    17401 cactcagcct ggggcaccga cacacataca gggaaaaata ggcacccaga cacagcctac
    17461 acacctacag ttactgatcc aaagagattg agatggcgca acgcaattaa tgataacttc
    17521 gtatagcata cattatacga agttatggtc tgagctcgcc atcagttcaa gatacttgtg
    17581 agtatggcaa ggtctctgag gctagacgta gctgatggag atgggatcag gccttctgaa
    17641 gcctgaaaag actgggggtg tggctggggg tttcatggat ggatggatgg atgaatgaat
    17701 gaatgaatgg atggatggat gctatcacca accccatctg tgactagctc agctctgtgg
    17761 ggctctctgc tcaaccctgt ccagaagaag aacccgtgtc ttccgttttc agggctggga
    17821 tatcctagat gagagtatta tactcaccta atgttctctc gaaaatctaa gccaaggaac
    17881 gacagtcaag aactatcact agatctttgg tttttaactt agaagacaaa ctcaaaatgg
    17941 catggtaaca tgcatgcctg tagcctcagc ccttggaagg cagaaactga agaactgcct
    18001 caattttcaa gccagcctac actacacagg gggtttcagg ctatccaggg ttacacatca
    18061 agaccctgtt ttaaaaacta acacaacaga gctagtgaga tggctcagca ggtacaggta
    18121 cttgcagcta aagctgaact cacattcaat tcctacagcc catgtggaag gagaaaatca
    18181 actcctataa gttatcccct gaactacaca tacacacaca cacacacaca cacacacaca
    18241 cacacacaca cacacaccta acaaaacaaa aaaaagaaaa atcaatgtag ctgtgcatac
    18301 ctgtaatccc agtactccag aggctgaagt aggaagatca tgagttcaaa gacagccctg
    18361 actgtctttg acatagtgct atcagcatga actgcaaatt caaattcaaa gtaatacatc
    18421 ttatttgctc tgtgtgtgtg tatgtgcatg tgtgaatgaa tacatagcat atgtgcccag
    18481 aggacaactt gcatcagtca gttctcttct tcccagagac tgagctcagg tcgtcaggct
    18541 tgacagatat tgtctgtctg tctgactcac ggagcatctc atcggctcca gacagtgatt
    18601 ttaaaaactc tctctttaat cgtcatgcca cctacttttg atttgttaaa gctttctttt
    18661 ttaaagattt actatttatt ttatgtattt gggtgttttt ctgcatgtac gtcttgcata
    18721 ctagaagagg gcatgggacg acatgggact acatgtgtag gactatagtt acagatgatt
    18781 gtgagtggct atatgggtgc tgggaattga acccaggact tctggaaggg cagccagtgc
    18841 tcttcactgc tgagccattt ctccagcccc ttgtgatagc ttttaagcca aaacatactc
    18901 cttatttgaa ccctgtatgg cttcagcttc tgtctttttc caaggagaga aagaaacaat
    18961 aagcaaacaa ataaaagagc cttccagaaa aagtcagaaa aaaaacttct tcctcacaga
    19021 ctgaccccac agatgggccc cacagtaccc agaattcctc caagccttag acagcaccct
    19081 tgcctgcctt gctccatccc agaccctcat gctgtcccaa gcctccttag ccccatggtt
    19141 tcccttctcc agcttgatcc tcagctgtcc ttgtaccttc ctcagagaac ggtgagtggg
    19201 agatagtgca tcgggcagcc aagctcaatg tggaccccag tgtcccgatg gacagcacca
    19261 accaccaaga tgtcaccttc taccttatca tccgccgcaa gcccctcttc tacatcatca
    19321 acattctggt accctgcgtg ctcatctcct tcatgatcaa cctagtcttc tacctgccag
    19381 gcgactgtga gtctcatgcc ccactcccca gctccccggc agcctctccc aaggccacct
    19441 agcttgggtg ttcccagtcc tgcccctgat cttcacggtg aggggagaaa tgaccattgt
    19501 gaataagaac tcaggcagga atcgcaggca ggcctagctg gtctttgggt taggaactgc
    19561 atgctgagat tcaaccagga tgtctgagaa ctttctgagt ggcagggact gggaacacat
    19621 aacagtcagt gttgatcact taaccagtga tatgcttgca atgttttgaa agaaaggcag
    19681 ggagtttaag ctcaggctac ttgcccagat cactcagtta attagctgtg gagccaggat
    19741 gtaaagctag gcacaggatg ccatacccta ggatacatat catggcattg tgaaccttgg
    19801 tggcagtgcc cgctgtgagg ggaccttgtg gactccaagt gtgagcttcc agctaggtct
    19861 gcctctccag gtggagagaa gacctccgtg gccatctcag tgctcctggc ccaatctgtc
    19921 ttcctgctgc ttatctccaa gaggctgccg gccacttcca tggccatccc cttggtaggc
    19981 aagtgagtat atacagccct gcccacagcc agccttgccc atgcctctgc cctcagtccc
    20041 cagctccagg catgctgtgt gctctcaggt tcctgctttt cggcatggtg ctggtcacca
    20101 tggtggtggt gatctgtgtc atcgtactga acatccactt ccggacgccc agcacccatg
    20161 tgctgtctga gggagtcaag aaggtgagct tttgaccaaa gatggaaacc aagtcctggc
    20221 cgggaggatc cgtgagcgtg atgggtgtga tctcagaatc actggtcctg ttgcccagag
    20281 tgaggattct cagctccact aaactcttta aaataataat aataataata ataataataa
    20341 taataataat aatggaccag ttagctagct cagtgagttg gagcattttc caccaagctt
    20401 gactgcctga gtttgatccc tatgacccat gcggtataag gggaggactg actcctgcag
    20461 gatgtcccct acctcctgta gtagcactgt ggcatgtgta tacatgcaca cacatatgcc
    20521 cacatacacg tgtgcatgaa tactatcatt ttaaaattaa aacaaaattt aagagtaagc
    20581 aggcgaggac acaggggaca gggtgtttgg gacaagacta tctgtgctct aagattgtgg
    20641 gacaataggt tcttctggaa taagtcatct tctggatggt gattctatag ctccttaatg
    20701 gtttttgtct ttaattgaaa ggagagccca acatttgggc ttttattcat ttgagacagg
    20761 gccttactat ataactcata acagaggggg gcaccggtgt atagctcagt tggtacagtg
    20821 cttgcctagc ttgcacaagg cccaatactg agtatgaacc ctgcttgatc ctagtgggag
    20881 gatcagaagt tcaaagttat cctcagttac atagtgagtt ctgggctatc ctagtataca
    20941 tgagaccctg gcaggaagga aggaagggag ggagggaggg gagggaggga gggaggggag
    21001 ggagggaggg aggggaggga gggagggagg gagggaggga gggagggagg gagggagaga
    21061 gggagggagg gagagaggga gggagggagg gagggaggga ggaaggaagg ggaagggaaa
    21121 gaggatggag gaggaggagg aggaggagga ggaggaggga gaatcagggc agaaggaagc
    21181 tgaggtgtgt atcagtgata aagcatatgt tcaaactctt aattctcctg cctcagcctt
    21241 ctgaatgcag ggattgaagg catgtacccc cattctcagc ttcttttttt cttttggggg
    21301 ggtgggtgtt tcgagacagg gtttctctgt atagccctgg ctgtcctgga actcactttg
    21361 tagaccaggc tggcctcgaa ctcagaaatc tgcctgcctc tgccacccga gtgctgggat
    21421 taaaggcgtg tgccatcacg cccagcgctt ctttttttct taagatttat tttcagtgac
    21481 tgagttctgc tgttgctgta agtaatgagg gaccgttgaa agcattctac accagtcagt
    21541 ggggagtctg ggaaacagac aatggggtca gcctgggagc tctatgcggc aaccagggtc
    21601 ctcagaatgt gtaaagatga ctccacactt gtgaaatggc ctgtcttgtg tccccctgac
    21661 tgctaaagtt cttcctggag accctgccca agctcctgca catgtcccgc ccagcagagg
    21721 aagacccagg ccccagggct ctcatccgga gaagcagctc tctgggatac atctgcaaag
    21781 cagaggagta tttctcactt aagtcccgca
//