LOCUS       87215#pL1L2_GTK_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        22965 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GTK_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 87215
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000164614
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8676..8819
                     /db_xref="ENSEMBL:ENSMUSE00000556147"
                     /rank="2"
                     /note="ENSMUSE00000556147"
     misc_feature    4275..10313
                     /note="5 arm"
     misc_feature    10341..10365
                     /note="B1 Gateway"
     misc_feature    10378..10425
                     /note="Frt"
     misc_feature    10426..10926
                     /note="mouse En2 intron"
     primer          complement(10494..10515)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10390..10413)
                     /note="FRT3"
     primer          14429..14447
                     /note="seq primer NF"
     misc_feature    14489..14496
                     /note="RE sites"
     misc_feature    16289..16322
                     /note="loxP"
     misc_feature    complement(14497..14528)
                     /note="Rox"
     misc_feature    complement(16249..16280)
                     /note="Rox"
     misc_feature    14529..14550
                     /note="polylinker"
     CDS             15109..15705
                     /note="puromycin N-acetyl transferase"
     misc_feature    15224..15229
                     /note="Mutated RsrII site"
     misc_feature    11463..12179
                     /note="EGFP"
     misc_feature    11373..11462
                     /note="3X NLS"
     misc_feature    14245..14483
                     /note="SV40 polyadenylation site"
     misc_feature    13278..14222
                     /note="ERT2"
     promoter        14561..15072
                     /note="PGK promoter"
     misc_feature    15816..16248
                     /note="PGK pA"
     primer          14303..14326
                     /note="LRPCR RAF5"
     misc_feature    12189..12242
                     /note="T2A"
     misc_feature    12239..12239
                     /note="A to G to kill RsRII site"
     misc_feature    12243..13268
                     /note="Cre"
     misc_feature    11201..11222
                     /note="seq primer FCHK2"
     misc_feature    10927..11363
                     /note="mouse En2 exon"
     misc_feature    11315..11383
                     /note="FCHK cre_ert_gtk"
     misc_feature    11315..11350
                     /note="FCHK QC prefix region"
     misc_feature    11351..11363
                     /note="FCHK QC critical region"
     misc_feature    11364..11383
                     /note="FCHK QC suffix region"
     misc_feature    16345..16365
                     /note="B2 Gateway"
     misc_feature    10314..16395
                     /note="Synthetic Cassette"
     exon            16749..18617
                     /db_xref="ENSEMBL:ENSMUSE00001290650"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00001290650"
     misc_feature    16396..18769
                     /note="Critical Region"
     misc_feature    18793..18826
                     /note="Downstream LoxP"
     misc_feature    18770..18849
                     /note="synthetic loxP region"
     misc_feature    18850..22965
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatgacca atcactcttg agaactggga ggataatatt aaaaaagagg
     4321 gctagtcctg atcaatggca tactgctcaa cttggaaatc cctaacctta gagactgccc
     4381 tagtagctct cctttctcag ccctcccacc tgtcactgta gtctgtgaga cctgtctatc
     4441 tgtctgtact tcttttttaa agaattatta ggggctggtg agatggctca gcaggtaaga
     4501 gcacccgact gctcttccga aggtcctgag ttcaaatccc agcaaccaca tggtggctta
     4561 caaccatccg taacaagatc tgactccctc ttctggtgtg tctgaagaca gctacagtgt
     4621 acttacatat aataaataaa taaataaatc tttttttttt ttaaaagaat tatttatttt
     4681 atgtatgggt atgagagtgc actgtcactg tcttcagaca cacctgaaga gggcatcaga
     4741 ttccgttgtg agccaccatg tggttgctgg gaattaaact caggacctct ggaagagcag
     4801 tcagtgctct taatcgctga gccatctctc cagccggaga cctgtctttc tggcagggct
     4861 ctagacacat accatgttcc ccaagccggt aaggctcaga gcctccaccc aaactggggc
     4921 tttgtcttcc tgggtcccat gtcatatgcc atcagaactc accccatcca agcctgagtt
     4981 cctctggtgg cctgaaaacc ccagcagact tgcccatggc tcactaatgg ctcactaatg
     5041 aggctaccac caccaccagt ccctctttgc aaggatctcc tgggactccc actgtacagc
     5101 ccctgctcag ctactcaggc tccatatccc tggttctcct aggtgtgtcc cacgaaaccc
     5161 tgtttggttg ttgaatagag tcttatgcgt ttttcttgca ccttatgaga ggacccctgg
     5221 ttggggctgc atatcatgtc tgcagaggtc tggggaggga acaggtgggg taccacacat
     5281 aggaagcctg ggtggggatc ttgcctgagt gaccacctct cttcttgtgc ctgtatcccc
     5341 atcagatcgc ctgactctcc cttggctcat tctgttggca ctcagcccag aggtgagatc
     5401 tatggggttc aatgtgggct gcatctgacc atccccatga caccatcttg gctcccagtc
     5461 agccagggaa acagatggag gtacatccat taagagtgtt gcacttgagt tggaaaacag
     5521 tgctggacct ggtgccaagt tctgtctctg ccagcctgta tttctgccca gtctcactgc
     5581 ctacagtgaa tcatagagag tcctgataac agggagccgg ggtaaggctt agagcagcac
     5641 tatgaccagt taacacacta cctgaaaata gctccctgcc ctagggaagg aagcctaggg
     5701 gtaccgggga ggtaacaggg acagcccagg ctcttctgac aagggctagg acttgggggt
     5761 gcagctgcaa ggcccaccca gtgggggatg ggaccatgct ttgagtctaa agtcacatgg
     5821 gtattgagtt gtggccaact tggctgggta cataacttct attcctggat cttcagctgg
     5881 gggccaatga taccaaatat ggcctgattc tagcaccttc tagttgtggt caggcacacc
     5941 ttaccccacc tcaggcctgg tggtcggtct ccaccctatg ccatggtctt aaatactcag
     6001 gggtacactc tgctgatggc tgtccataca tccatctacc tgtagtccta ctgggttccc
     6061 agcacccagg gaatatagga ggcagatctc tgagctccag gacagccagg gctacacaga
     6121 gaaaccctgt ctttctaaaa aaaaaaaaaa tcaaaatgct tatgaccaaa atagcctcat
     6181 tccccaaagg aggaggacag agctggtgac ttcccagggt ctgtctcgct gtgggctcag
     6241 ccacacatac gactctggtt gggctcccaa ggagctcact gaccttggtt ccagcctcac
     6301 tggctccctg gtgctcttgg tcatttagca ccccccaccc tgctcagcca ctgatctgct
     6361 ggctcccaga cttcctcacg tcttgcttca ttcactgcct ctcctacctg tccaacaggt
     6421 tgacaacacc tatcgagctg gataggattc cagatgctaa acaagtacaa caaaatacaa
     6481 atccctgaat gagcttctca tcccagcagg aagggaagtt gggagcaaga tcctgagttc
     6541 gaggctagcc tgagccactg cctcaaagac aacaaaaaaa cacaaggctg agatatcagc
     6601 tgagttggta gaacgtttgc cttgctcacg tgaagcattg tggaaggagg atcagaagtt
     6661 cagggtcatc tttgggctat atagtaaatt ggaaactagg aaactagtct gttctacatg
     6721 agttgctcaa taaaacaaca tatctaccct tgttgggtgt cttagggttt cattgtgacc
     6781 atgatgactt tttttttaaa cgaattagtg ctctgtctgc acgtatacct gcgtgccaaa
     6841 agagggcatc agaccccatt atcgatggtt gagccaccat gtggttgctg ggaattgaat
     6901 tctgaacctt tggaagagca gcaggcttaa ccactgagcc atgtctccag cctgaacatg
     6961 gtatactctt ataaaggaaa gcatttaact ggggttggct tagagtttca gaggtttagt
     7021 cccttatagt catggtggga agcatgatgg cacacagaca gacatgatgc taaagaagga
     7081 gctgagagtt ctacatcttg atatgcaggc aggcagggag agaaagacac tgggtgtggc
     7141 ctgagcagct gacctctacc ccatccccaa gtgacacaat ccaacaagtc cacacctcct
     7201 aatattgcca ctccctatgt ggccaaggga gccattttca ttcaaattac catattgggc
     7261 tatttctaaa taatttacct gtacagtaag cacagtgttt tgggataagt gctagggaga
     7321 ataaaccaaa gtggaaggca ggggggagct ctagagtgaa gtattcgagg cttgagagca
     7381 agttaggagt ggagagtgag ccacccaagc agagagggtg gctagtcctg aggcagcata
     7441 ttgagttctg agacactgga aagacagatg gcttagatag ataaggagcc agggagaggg
     7501 tggtatgaca gaagacacag gtataaggca tatacagggc cgggggacca gagtgtctta
     7561 aggtctgttc aaggccattt gaacttacta taatagccta ggctagtctt gatcctcttg
     7621 tctcatcttt gtagtgatga aaacatagtt tggttggttt ggtttgattt ttcaagacac
     7681 agtttctcta tgtagtcctg gctgtcctgg aactttaaga cctctcttta agaccaggtt
     7741 aatttggaac tcagattcac ctgccttgcc ctcttaagtg ctgagattaa agtcagatta
     7801 aaacattttt aaaagatgtg ccagccgggc agtggtggtg cacgccttta atcccagtac
     7861 ttgggaggta gaggcaggag gatttctgag ttcgaggcca gcctggtcta cagagtgagt
     7921 tccaggacag ctagggatac acagaaaaac cctgtctctc gaaaagccaa caacaacaaa
     7981 aaaagtgtct atttattatt tatgagcaca ctatgactgt cttcaggcac atcagaagag
     8041 ggcaccagat cccattacag atggttatga gccaccatga ggttgctgag aattgaactc
     8101 aggacctctg aaagggcagc tggtgctctt aaccactgag ccatccctcc aaccccccca
     8161 gattaaaaca tttttaagag tcatttcatg tatatgtatt tatctctctg tgtgtatgta
     8221 tgcatgtgat tgtaaacagg gccagaaagg gagggggtac tgtgtgtctt agaggtggag
     8281 ttgtaggcat ttgtaagcca ctagttgtgg gatctgaact ccggtctgac acagccacct
     8341 tccttggtac ctcccattgt cccttccagc ctatggtgcc aacaaaggtg caggctccta
     8401 ctgaccagtg cctccaccat ctgactgttc ttcccttctc ttcccgatcg atgcccagcc
     8461 caagtgtcac ttcacccagt gagttctagt caattggact gaccctgttt ctagacagtg
     8521 ttcccacagg gctgagcata ggcaaatgtg ctcagtttga caacagggcg ggcctctaaa
     8581 agccagagct gagtggcttg cccttctgga tggaagggag tggttgctga tggcctctag
     8641 ctgtcttgga cttttttgcc ttctatcttt tgcaggtggg tgtgacaggc ctgagcatga
     8701 gaggacaagc tatatactcc ggttggcaca gaagactagt tcctaaccca gtgtgcatgt
     8761 ccaggatgcc tgcaggctgc taggacccct cacctccttg ttatggactg cccaggctgg
     8821 tcagtagctc tttatcctgg acggagttca gtatgttggc tcaaacccca tccctgtgag
     8881 atgataattc caagagtcct ggagggactc agttctcagt tcccagtagc atctacctga
     8941 ctgggtctag tcctgtgacc tctttccact caaaatttct ggtttctaat gtccaaataa
     9001 tgcgcaattg gccaggtggt agtggtgcat gcttttaatc tcagcacttg ggaggcagaa
     9061 gcaggtggat ctctgagctc aaggttaacc tgatctctgg tttatagagc gagtaccaga
     9121 acatcacatc cagggctata cagagaaatc ctgtctccca aaacaaaaca aagcaaaaca
     9181 aaacacacac acacaaacca agcaaaccaa aaaagcccac aaaacagatg caactgtcct
     9241 ctggagtggc cgagggtctg aggggtgtga ctgcagccca gaacatgcag caggtatggt
     9301 gttaaagttc aggtcatagg gcagagttca tccagcaaac cagacacacc cttagctact
     9361 gtcttccagg accagctatt gctccagaga gcccaccctg cccactcctc ccatgcacat
     9421 cctgtcactg tctgcctggg gacagcttga tgactataga ctgtctattt cctcatcctt
     9481 aaggcagagg cgaggagagg caaggattaa cctggtcagg ttgcctgtaa gggtttaaca
     9541 ggtttggatg tgtgaaatgc taaatttatt gaaagagcaa atgtaagaag cagtagagca
     9601 tggaggctga ggcgggagga tcacgagtga aaaaccagcc aggtctatat agtgaactcc
     9661 catcctcacc aacttagaaa gtccgccagg tgtagtagca cacgctgcca atcctgtagg
     9721 agacctgagt ttggtcccta agacctatgt tgggcagctc acatccacct gtaactctag
     9781 ctgcaggggc tccaatgctc tcttttggtc tctgcacgca tctacaggtg tgcacgatac
     9841 tcaccccacc ccaccccaat aattaaaaat gaaataattt gagtctaaag agctggtcca
     9901 gtggttacaa gcactggctc tcttccagaa ggctcgagtt tgatttctgc aactcacttg
     9961 acgccactca aaaccttctg tttccagtcc caggggatcc agtgccctcc tttggtcttc
    10021 aagggtgctc atgcctgtgg tacaccaata gacacacagg aaaaacaccc atacacctaa
    10081 aataaatagt atataataaa taaatcctta gtgaaaaaga agcgcatggc ccaagtatcc
    10141 ttccgtctct atccccccgg ggttggggtt ctaggcgtgc actgctgttt ctgtcttttc
    10201 cacgggttcc ctggatccga acccaggtac tcgtactaga catgttaccc actgagacat
    10261 ctctgccacc ctaagcccag gtcttcatgc caaatgtgtg tgaggcattt agtaaggcgc
    10321 ataacgatac cacgatatca acaagtttgt acaaaaaagc aggctggcgc cggaaccgaa
    10381 gttcctattc cgaagttcct attctctaga aagtatagga acttcgaacc ctttcccaca
    10441 ccaccctcca cacttgcccc aaacactgcc aactatgtag gaggaagggg ttgggactaa
    10501 cagaagaacc cgttgtgggg aagctgttgg gagggtcact ttatgttctt gcccaaggtc
    10561 agttgggtgg cctgcttctg atgaggtggt cccaaggtct ggggtagaag gtgagaggga
    10621 caggccacca aggtcagccc ccccccccta tcccatagga gccaggtccc tctcctggac
    10681 aggaagactg aaggggagat gccagagact cagtgaagcc tggggtaccc tattggagtc
    10741 cttcaaggaa acaaacttgg cctcaccagg cctcagcctt ggctcctcct gggaactcta
    10801 ctgcccttgg gatccccttg tagttgtggg ttacatagga agggggacgg gattcccctt
    10861 gactggctag cctactcttt tcttcagtct tctccatctc ctctcacctg tctctcgacc
    10921 ctttccctag gatagacttg gaaaaagata aggggagaaa acaaatgcaa acgaggccag
    10981 aaagattttg gctgggcatt ccttccgcta gcttttattg ggatccccta gtttgtgata
    11041 ggccttttag ctacatctgc caatccatct cattttcaca cacacacaca ccactttcct
    11101 tctggtcagt gggcacatgt ccagcctcaa gtttatatca ccacccccaa tgcccaacac
    11161 ttgtatggcc ttgggcgggt catccccccc cccaccccca gtatctgcaa cctcaagcta
    11221 gcttgggtgc gttggttgtg gataagtagc tagactccag caaccagtaa cctctgccct
    11281 ttctcctcca tgacaaccag gtcccaggtc ccgaaaacca aagaagaaga acgcagatca
    11341 taactgacta ggaggccacc atgggcggac cggtgcatgt ggaccccaag aaaaagcgga
    11401 aggtggaccc taaaaagaaa cgcaaagtgg atcctaagaa aaaaagaaag gtgcacgtgt
    11461 ccatggtgtc caagggcgag gaactgttca ccggcgtggt gcccatcctg gtggaactgg
    11521 atggcgacgt gaacggccac aagttcagcg tgtccggcga gggcgaaggc gacgccacat
    11581 acggaaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccttggccta
    11641 ccctcgtgac cacactgacc tacggcgtgc agtgcttcag cagatacccc gaccatatga
    11701 agcagcacga cttcttcaag agcgccatgc ccgagggcta cgtgcaggaa agaaccatct
    11761 tctttaagga cgacggcaac tacaagacca gggccgaagt gaagttcgag ggcgacaccc
    11821 tcgtgaacag aatcgagctg aagggcatcg acttcaaaga ggacggcaac atcctgggcc
    11881 acaagctgga gtacaactac aacagccaca acgtgtacat catggccgac aagcagaaaa
    11941 acggcatcaa agtgaacttc aagatccggc acaacatcga ggacggctcc gtgcagctgg
    12001 ccgaccacta ccagcagaac acccctatcg gcgacggccc tgtgctgctg cctgacaacc
    12061 actacctgag cacccagagc gccctgagca aggaccccaa cgagaagagg gaccacatgg
    12121 tgctgctgga attcgtgacc gccgctggca tcaccctggg catggacgag ctgtacaagc
    12181 ctgcaggcga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccgggc
    12241 cgtccaattt actgaccgta caccaaaatt tgcctgcatt accggtcgat gcaacgagtg
    12301 atgaggttcg caagaacctg atggacatgt tcagggatcg ccaggcgttt tctgagcata
    12361 cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag ttgaataacc
    12421 ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta tcttctatat cttcaggcgc
    12481 gcggtctggc agtaaaaact atccagcaac atttgggcca gctaaacatg cttcatcgtc
    12541 ggtctgggct gccacgacca agtgacagca atgctgtttc actggttatg cggcggatcc
    12601 gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg
    12661 atttcgacca ggttcgttca ctcatggaaa atagcgaccg ctgccaggat ataaggaatc
    12721 tggcatttct ggggattgct tataacaccc tgttacgtat agccgaaatt gccaggatca
    12781 gggttaaaga tatctcacgt actgacggtg ggagaatgtt aatccatatt ggcagaacga
    12841 aaacgctggt tagcaccgca ggtgtagaga aggcacttag cctgggggta actaaactgg
    12901 tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc gaataactac ctgttttgcc
    12961 gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag ccagctatca actcgcgccc
    13021 tggaagggat ttttgaagca actcatcgat tgatttacgg cgctaaggat gactctggtc
    13081 agagatacct ggcctggtct ggacacagtg cccgtgtcgg agccgcgcga gatatggccc
    13141 gcgctggagt ttcaataccg gagatcatgc aagctggtgg ctggaccaat gtaaatattg
    13201 tcatgaacta tatccgtaac ctggatagtg aaacaggggc aatggtgcgc ctgctggaag
    13261 atggcgatct cgagccatct gctggagaca tgagagctgc caacctttgg ccaagcccgc
    13321 tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg
    13381 tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac
    13441 ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc
    13501 acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg
    13561 tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca
    13621 tggagcaccc agtgaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa
    13681 aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc
    13741 gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt
    13801 ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc
    13861 accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga
    13921 ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc
    13981 acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc
    14041 tctatgacct gctgctggag gcggcggacg cccaccgcct acatgcgccc actagccgtg
    14101 gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat
    14161 cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagctt
    14221 gatgaagatc tgagctctta attaatgatc ataatcagcc atatcacatc tgtagaggtt
    14281 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
    14341 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    14401 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    14461 atcaatgtat cttatcatgt ctggatccgt ttaaactaac tttaaataat tggcattatt
    14521 taaagttaca tatggcggcc gccctaaggc tcgacctcga aattctaccg ggtaggggag
    14581 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
    14641 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
    14701 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
    14761 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
    14821 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
    14881 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
    14941 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcttc cggaggcccg
    15001 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
    15061 ggcctttcga cctgcatcca tctagatctc gagcagctga agcttaccat gaccgagtac
    15121 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
    15181 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccagatcg ccacatcgag
    15241 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
    15301 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
    15361 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
    15421 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
    15481 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
    15541 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
    15601 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
    15661 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac
    15721 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgatgat atcagatccc
    15781 cgggatgcag aaattgatga tctattaaac aataaagatg tccactaaaa tggaagtttt
    15841 tcctgtcata ctttgttaag aagggtgaga acagagtacc tacattttga atggaaggat
    15901 tggagctacg ggggtggggg tggggtggga ttagataaat gcntgctctt tactgaaggc
    15961 tctttactat tgctttatga taatgtttca tagttggata tcataattta aacaagcaaa
    16021 accaaattaa gggccagctc attcctccca ctcatgatct atagatctat agatctctcg
    16081 tgggatcatt gtttttctct tgattcccac tttgtggttc taagtactgt ggtttccaaa
    16141 tgtgtcagtt tcatagcctg aagaacgaga tcagcagcct ctgttccaca tacacttcat
    16201 tctcagtatt gttttgccaa gttctaattc catcagaagc tggtcgagta actttaaata
    16261 attggcatta tttaaagtta ggcgcgccat aacttcgtat agcatacatt atacgaagtt
    16321 atgtcgagat atttaaatat tagacccagc tttcttgtac aaagtggttg atatctctat
    16381 agtcgcagta ggcggaagag gaggcagact aggtcaggca gagggtgtta tgtggcaaag
    16441 tcattgcttc tgactgccct ttcacatgct agacattggc tgtacactct gagacacatc
    16501 gcttctggtc cctacgttca cgggtttatg atacaagggg caaaaaggac cttgcccaga
    16561 ccctgcctac ggttcccatg tcacagatga gcagccagcc tgccagggta caactcatgc
    16621 ctcatgtgtc ccagagaccc tgggctttgg aatattatct ttccctggcg tcagtaggaa
    16681 acgtgtttct tgagggtcac agatgcctac gtctggccta cctgaatgct ggtgctcatg
    16741 ttttacagac aagtcaatct ttatgggcat ctcctgttcc catctggagg agaccatgtc
    16801 caagcccccc gactgcctgc tgcggatgct caggggcacc cccagacagc gggtcttcac
    16861 cttcttcatc atcagcttca agttcatgtt cttgatctcc atcctgatct actggcacac
    16921 tgtgggtgca cccaaggacc aaagagagta tagcctgcca gtagacttct cctgccccca
    16981 gctggccttt cctagagtct ctgccccagg caacatcttc ttcctagaga catcggacag
    17041 gaccagtccc aactttctat ttatgtgttc tgtggaatca gctgccaggg cacaccctga
    17101 atcccaggtg gttgtgctga tgaaagggct gcctagggac accacagctc agccccggaa
    17161 cctcggcatc tctcttctga gctgttttcc taatgtctgg atacgacctc tggacttgca
    17221 agaactgttt gaggacacac cactggcagc ctggtactcg gaggcacggc acagatggga
    17281 gccctaccag ttgcccgtac tgtctgatgc ttccaggatc gcactcctct ggaagtttgg
    17341 tgggatctac ctggacacag acttcatcgt cctcaagaac ctgctcaacc tgaccaacac
    17401 gctgggcatc caatcccgct atgtcctcaa cggtgccttc ctagccttcg agcgcaagca
    17461 tgagttcttg gcactgtgtc tacatgactt cgtggccaac tacaatggtt ggatttgggg
    17521 tcaccagggc ccccaactgc ttacccgagt cttcaagaag tggtgttcca tccaaagcct
    17581 ggaaaagagc catgcttgcc gtggggtcac tgccctgccc cccgaggcct tctaccccat
    17641 cccctggcag aactggaaga aatactttga agacatcagc cccgaggagc tgacccagct
    17701 gctcaatgcc acctatgctg ttcatgtgtg gaacaagaaa agtcagggta cacacctaga
    17761 ggccacatcc aaggctctgc tggcccagct acatgcccgc tattgcccta cgacacatag
    17821 agccatgaag atgtacttgt gacagccctt caggctgcag tcatatcata tcaacattgt
    17881 taccgccttt ccagggagac aagatgagca gacagggaaa aaggggcctt tgagatgctg
    17941 ctgttgactt tagaggagac caagctggcc tggggaacac ttgccagttc tacacctgcc
    18001 cctctaagcc aaagggagac cgatggagaa cactttgtcc ggtgttctag gctgaccagt
    18061 gattacagaa ttactaacat ctggggcctt aatgggctag atcacaagaa cactaaagag
    18121 cattctaagg tagctggaag acagctggtt agaggtgggg tcaggagcgg agctctgaaa
    18181 acctgtgtag aaatgctgag ggagaaggac ctggctctct cagcctcatg agcccaaggg
    18241 gaggggcagg gctccctggg aacaccatct ttcctcccct gggacagtgc tctgaagatc
    18301 tatcacagcc tgttccagtg ttcggggatg tggacttttt tttttttttt ccgagacagg
    18361 gtttctctgt gtagccctgg ctgtcctgga actcactctg tagaccaggc tggcctcgaa
    18421 ctcagaagcc ctcccagtgc tgggattaaa ggcgtatgcc accactgcct ggcttttttt
    18481 ttttttttaa ttttttaatt ttttattttt atttttattt tggtttgtgg aaattcttta
    18541 ggttttaaaa aaggaaaaag agttttaaac tccctccctg ttgtagacac tgggggctct
    18601 aataaaagtg actcaccaac atgtaatgcc tcatggtcac atcgtgcact tgtgaacgta
    18661 gacttctgca cacactgttc cacagatggg aacaggctgg agagtggcca tttcattggg
    18721 gtcacaaaat tcacttggag aggctgtgct cagaactggg tggctggctg agatggcgca
    18781 acgcaattaa tgataacttc gtatagcata cattatacga agttatggtc tgagctcgcc
    18841 atcagttcaa ataagcctag atttatttta gcctaagtgg ggcttcgtat aaacaccaag
    18901 gcgtgcgaat aggtgctagc cacagccaag cctggaaggg aggggaccct ggcctcacct
    18961 tggaagtctg gccaggctac tggtaacttc tggaagtgtc ctgctatgca gtactaggaa
    19021 caacatctct gtagccaata ctgattcttc tcttggatgg ctgggcagcc ccatggggtg
    19081 ggaagccagg aggctgctgg cagctctgga cttctctcca gcctctgtgt tctgcctgcg
    19141 ctcaggcaga tgagttgtct ggttccaaag gcattcaaga gcagatgaga ggtttttggt
    19201 aaagacaacg aattgtaagc caattatttc cctagtcaat catttgaaaa ggtctcaact
    19261 gtagaggctg gggagacagc gcatccatta agagtgcctt tggcactttc ccagaagacc
    19321 caagattggc tgcctaagtt tggtcagaca ttctgtaggg cagctcacaa ctgtgctgta
    19381 actccagctc caagagatct gacgtcttct ggattccact agctcctgaa cacacatgtg
    19441 cacatacaca catatacaca cacacgaatc tctatagtcc aggctgtctg gtgtgaagtt
    19501 taccattaag tctcctgttt cgttctccca agaggggact ataggcatga gccactttgc
    19561 ccagtgtcac ctatctgtaa tttctttctg ttttagagtg acaggcatta ctcttaacaa
    19621 cacaagcact ctttgtgatt gtacatgtat atggaaaaag tgagatgatc ccttagggat
    19681 gggggatgtt cagcctcatg atggtggtag atagagtgac accctgaacg tgcttgcaca
    19741 tgggagggaa atacatcact ccctcgccat aatctcttcc ttagctccct gtttgcaaaa
    19801 caaaacactt aggccctgca ttcaaggcct tccataactt ggccccgccc cctgactcca
    19861 tccacactaa agtcatatat tcttggctcc tgtcttgggg tttctattcc tgcacaaaac
    19921 aacatgacca agaagcaagt tggagaggaa agggtttatt cagcttacac ttccacattg
    19981 ttgttcacca ccaaagaaag gacaggaatt caagcaggtc aggaagcagg agctgatgca
    20041 gaggccatgg agggatgtta cttactggct tgcttcccct ggcttgctca gcttgctttc
    20101 ttctagaacc caagacgacc agcccaggga tggcaccacc cacaaggggc cctctccgct
    20161 tgatcactaa acgagaaaat gccttacagc tggatctcat ggaggcattt cctgtgataa
    20221 ctccagctgt gtcaagttga cacacaaaac cagccagtac agcctcttag aactctttct
    20281 catagtagca tcttgacacc acactcctgc catgttgcct gcagaacctc tcccaaccat
    20341 cattaccacc tacgatgcag acagcctctt aggaagcctt ctgggactga gcctcctcca
    20401 gctcctcagc acctgggggt aatcccagct tagccgactc tcagccggtc tcagtaaagg
    20461 gcctagaaag tgagactggg ctaagtgcat ctctgggttc ttcccaggat cagactcaga
    20521 atgccatgta gctgggtgag ggtctcaaaa tcttatcccc agcagagctg agagctcaga
    20581 agggctggtc ttagtctact gcttgctcct ttgggtgcat cggtctgaga cttggccaca
    20641 tggcccctcc gcacagtgga tagagcagtt gcagagtcct ctctgaggtc ctgtgggcag
    20701 gccttcctga cggatgcagg aggcagccct ggtcccagac cttcctcaag tcatgcctag
    20761 ctgggccata tctagaagca gagatccaaa ttagcaagtg aacagagagg ttcttcatct
    20821 tcttccaccc ttcatccaaa cagcctgtgt cttgcccagc cactccactc tggaccttat
    20881 tccagctgcc ataaactgcc aaccaaaata cagcacagca gaaatagcag tgtaccctgg
    20941 agaactggca gaaaggcaga cggggcctgg gtgacaggag tgaagggctg actcattcct
    21001 agatgagaga gacgctttga ggcccggtga ggggaatctc ggcatccacc atcactggcc
    21061 gctttcatgc acttcctggg ctgggatgtc ttggttagtg tccagcttaa aagccctctg
    21121 ccagctgcca gcctctgagt tggggccagt tttgacttgt tcctgttttt ttttcgaaaa
    21181 acaggagctg agctatgctg gttcccagca tggatgccac tagcaaagga gccacactgt
    21241 gtgcacccaa atccttcatt ttaacatccc atctcccaca tcagggagtg gaggttcggg
    21301 ctagacccta tgtgatttta gagataagtg caggcttgtg gggctgtgtg ggctgggagc
    21361 tggccattcg tataaacttc aatgcaccca cagacccaca gactagaccc tcagctcctg
    21421 cctcacacac aactccctgc gggacccctc accctactct ctgctagggc ctgattctag
    21481 ataccctgcc agcattttcc cctgccctgg cccaaccctc cagagtgcgg ttaccctggc
    21541 aacctccagc tgctgctttc tgggggagag gtcagagctg ctacacagct ggattctgag
    21601 aatgggaaag ggaggggttg cctgggtgga accagtcccg gggacaggac gtgaaatcag
    21661 atcctcagcc ctgtggtttc tcactggcta gttggcttat agatattttt gttcatggct
    21721 gagggagaca gaacccataa ggggcccaag gcaagctatg gatctgtcac ctagtgaaga
    21781 ttccaggctg gccagccagg cactggccag caggaaagtc tttcccagca gctcagggct
    21841 agctagggaa ctaatttccc ctgactgcta tatcaacatt aagcctcttg tttaaatatt
    21901 aaatcggacc gccctccctc tgactgccta aacagaggcc aagggttgat gagcaccgag
    21961 ggccccgttc ctggaagtct gctttcttgc tggacatctt acaaatctct gcttaggccc
    22021 agttgggcgc taggcatgct gggaattttg tgagggtgga ggtctggagt gggcaacaat
    22081 gattcaagtg tgatctctcc gggagcaggg tggcagaggg agcgagcagg agctacttag
    22141 ggtctcttgc tgtgataaac accatgacca aaagtgacct ggggaggaca gggtctattt
    22201 catctttcag tttgtagtcc atcatccaga gaagtcaggg caagaattca aacaaggcag
    22261 gaaccacctg gaggcaagaa ctgatgcaga ggccatggaa agtgtgatgc ttactggctt
    22321 gctcctcatg gctagctcga catgctctct tatggaacca gtgaccgcct gcccagggat
    22381 ggccccaccc ctaacaggct gagcccttta atagcattaa ttaagaaaat acacccaggc
    22441 tggctcacag gccaatctgg tagaggcatt ttctcaattg aagggttttt ttcttccaaa
    22501 atgatgttcc ttttgtgttc agttatcata aagccagcca gcataaagca cttcagtaga
    22561 gcctgggcag gagaaagcca tttggtggtg acagtgaagt aaagaccaga agccgttcct
    22621 gatgtccttg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg ttgttttttg agacagggtt
    22681 tctctgtata gctctgactg tgctggaact ctgtagacca ggctggcctc gaactcagaa
    22741 atccgcctgc ctctgcctcc cgagtgctgg gattaaaggc atgcgccacc acgcccggcc
    22801 tgatgtcctt aattcaccaa caaaacacaa actccatcag cttccaacac ccaaagagaa
    22861 gtaagcgaga ccccagaggc atccagctgc cactgcacca gatgagaatc taatgtacat
    22921 ccgtgacaga ttggggacag tattcaggaa gctgcagtaa caaaa
//