LOCUS       186474#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21432 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 186474
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000048941
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..9933
                     /note="5 arm"
     misc_feature    9961..9985
                     /note="B1 Gateway"
     misc_feature    9998..10045
                     /note="Frt"
     misc_feature    10046..10545
                     /note="mouse En2 intron"
     primer          complement(10114..10135)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10010..10033)
                     /note="FRT3"
     primer          14211..14229
                     /note="seq primer NF"
     misc_feature    14271..14278
                     /note="RE sites"
     misc_feature    16071..16104
                     /note="loxP"
     misc_feature    complement(14279..14310)
                     /note="Rox"
     misc_feature    complement(16031..16062)
                     /note="Rox"
     misc_feature    14311..14332
                     /note="polylinker"
     CDS             14891..15487
                     /note="puromycin N-acetyl transferase"
     misc_feature    15006..15011
                     /note="Mutated RsrII site"
     intron          10546..10920
                     /note="mouse En2 intron"
     misc_feature    10918..10920
                     /note="Splice Acceptor"
     exon            10921..10974
                     /note="mouse En2 exon"
     primer          10821..10842
                     /note="seq primer FCHK2"
     misc_feature    10975..11097
                     /note="1Dleader"
     misc_feature    11098..11149
                     /note="F2A"
     misc_feature    11245..11961
                     /note="EGFP"
     misc_feature    11155..11244
                     /note="3X NLS"
     misc_feature    14027..14265
                     /note="SV40 polyadenylation site"
     misc_feature    13060..14004
                     /note="ERT2"
     promoter        14343..14854
                     /note="PGK promoter"
     misc_feature    15598..16030
                     /note="PGK pA"
     primer          14085..14108
                     /note="LRPCR RAF5"
     misc_feature    11971..12024
                     /note="T2A"
     misc_feature    12021..12021
                     /note="A to G to kill RsRII site"
     misc_feature    12025..13050
                     /note="Cre"
     misc_feature    10936..10975
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    10936..10951
                     /note="FCHK QC prefix region"
     misc_feature    10952..10969
                     /note="FCHK QC critical region"
     misc_feature    10970..10975
                     /note="FCHK QC suffix region"
     misc_feature    16127..16147
                     /note="B2 Gateway"
     misc_feature    9934..16177
                     /note="Synthetic Cassette"
     exon            16539..16657
                     /db_xref="ENSEMBL:ENSMUSE00001045639"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00001045639"
     exon            17167..17300
                     /db_xref="ENSEMBL:ENSMUSE00001021001"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 2 ENSMUSE00001021001"
     misc_feature    16178..17468
                     /note="Critical Region"
     misc_feature    17492..17525
                     /note="Downstream LoxP"
     misc_feature    17469..17548
                     /note="synthetic loxP region"
     exon            18223..18391
                     /db_xref="ENSEMBL:ENSMUSE00000983394"
                     /rank="5"
                     /note="ENSMUSE00000983394"
     exon            19103..19186
                     /db_xref="ENSEMBL:ENSMUSE00000304101"
                     /rank="6"
                     /note="ENSMUSE00000304101"
     misc_feature    17549..21432
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatccaca cagctagtaa atagcagaga caggagtcat acctacattt
     4321 tatgctatat tcccactaag ggaactgtac cccctcacta aagtcaacct gtaaaggcta
     4381 tgggaacctc tttccctctg gcgttttcaa agatgcagcc ccagccttga gtagcatttc
     4441 acactcttca tgagatgcag agccgccaac aaccagtatc cctctctgtt gactcattat
     4501 cttcagtcag tctcccttga aaagcatcgt acttgggtag gtatcgtgtt ttgaacatga
     4561 gaagtttgtc ttaaaaaatg tttttgtgtg tgtgtgcttg catggattta tgtgcatcac
     4621 atgggtgcag gagcccactg aggccaaacg aaggcatcgg attcactggt actagggtta
     4681 tagatggctg tgaggcactg ggtgggtgct aggaaccgat ctcatcctct ggaagaacag
     4741 tgagtactcc aagcctttga gccatctctg cagccaggag aactttatct taaagacaag
     4801 ttattcctaa tccatgatga gaacaaggct gtttgagtcc aaagtctggc tcccactcgc
     4861 ggatgggctt gccccgtaag tgttttcata tccctatccc tctgtgcttc tggtaagacc
     4921 cactccctcc caagcttctt cagatgccca ctctcttacc aaacagagac gctagtggga
     4981 aaggcaggcc taaagaccaa ccaaatctag acagctcctc actgagactc tctttccagg
     5041 cgattctaga ctgtgtcacg tggccaagca gatggccctc acaagagcga tccctcctgt
     5101 ctctggaagc acatctatgc ttgctggtgc tgaggagtgc ttgggctttc tcagcacgct
     5161 cctcttctgt gtgtcagttg atcgagagat tcatatgcaa atgctaatct ctgtaattcc
     5221 atactccatg attctctgtg gaggtcagac agacaaccca gccagtgtcc ccagcgcaca
     5281 gccagttcaa tcagcatata gaataatgtg tgtatttcaa gtatccctac ttctgatatt
     5341 ttaaagagct cctttagtag agtttctttt agaaccattt tacagtggca ttctaatcac
     5401 tttgttatta tctcaggaaa acgttggctg aaagaaccat taacctctgc ctaaacaaga
     5461 ggagatgagg aactcatagt tcgttgtgac aggagtctgg aggtgggtgg ccctgggcta
     5521 gttcttagaa atccagacac acactgggct cctgctctgt tttcccgagt gtctagactt
     5581 ccatcccact ctgtagccct gggtttctga gatgcctatg gatctccagg cataagtcag
     5641 acttcctagg taaggagaca ggggaagagc agtaggcaga aaatctgcca tgcttcatta
     5701 ggaaagcagg cgctactatc aaattcccac ttcaaactta agctcttagg aatgctaacc
     5761 tgaggtcact agccaggagg tggctgaaga gaagagacct gtctgtggac actttctgag
     5821 gctttcttgt ctacctcact taccaacaga tttgccaggg taaattccaa agctttgtga
     5881 ctgcttttgg aaatcaggac tatcctcaga gatctaggtg cctcggcttt gaggtaggcc
     5941 cctcaaacaa gtgtccaagg tgttttaagc agaagagtaa atgcctgtca ttccaagaaa
     6001 gcttttgaaa ggaagcacat ttgtttgggc acttcagaaa aagcctcctt gtcaggagga
     6061 gtcactcgaa cgtcatgtag tatttggcag ggatccaaat gtctatacct atggctttct
     6121 ttggatccca ttgtgcttac tgcagcagct gagtgggccc tgaaaaatgt caggaacgaa
     6181 agagagaaag aaacattaaa ctcatccttg gctgggcaag tggatgttag ccactgaggg
     6241 atgccttctg gcatgtcttc gtgtaatgtc caggaagcac tcagggtgtg gtgttgcagc
     6301 agcttaaaga ctgtgagaat gttcacatca gaagggagtc atggtcatac ctgccataat
     6361 ggctgagcag caagcatctc tggtttccct gagaagtcac ctgcttcaga gacactgcag
     6421 agcagtcatt ctgccccttt cctggggact gtgcccatct cacctgtcac ggagctttga
     6481 gtagccaagc ctcttggtgt gatgcgggtg tcatgtatga gagtcatgag gtttaatgca
     6541 tggccccgtt cacttcagcc cagaccttgg gtcgtcaggt gagaacacac gttaggcagg
     6601 cttattcact ctccaagggt cagaacgtga cccatgaact cctttggtca tttgctgaca
     6661 tgtttttctt tgtcaaaaat tcactgggtc atctctgtca gctactggga tgctactggg
     6721 agagactcca tgttccttct cggcaggagg agatcagata tcagggatga gagacccata
     6781 tcaggcagcc ttggtagggg ctaggagctc tgtggaggga gggcttggga atgtagggtt
     6841 aattagagat cacaaggaac atgccacggt ggagcctccc tgtgagtgga cttgggggcc
     6901 cagcttgtgc gctatgatca gctgagaggg ataaggaagg tctccaaaac atacaaagga
     6961 caaaagaagt gccttccgag tggaccccaa gttagtgggg gggggggggt gaagactcaa
     7021 ctgatcaagt tgcaggaagc accagagaga cacagacaga cagacagaca gacagacaga
     7081 aatacagaca gacagaacaa ataataaaag agatcaggca ggaaaagtga tcagggttca
     7141 cttgtgcttt cccaggctaa cctcacaatg ggcaaggaac caggcaggta gggtggagtc
     7201 gctctgggaa aggtttttat tttgtttcta agcaaagaaa ggaagagcaa tgacattgta
     7261 acatgagttc ctaggagagc ttacaagaag cagcagagct gaggggagcc cagtgcggga
     7321 agggaaatct gttgtttcca ctcttaggaa gttgcttgtt taatgactta ccaccctgct
     7381 gcagtctcct atgtaaggag caacatagtg cagtgctcat gcagaaacca tctccagtct
     7441 caccttgaac ttctccttga atccctgtga tcatctgcga ggtagcattt gctgcgaggg
     7501 aggtagagac catgccacct caaatgtcag aaaaattact caaaaagtaa ctatgtgtgt
     7561 gtgagagaga gacagagaca gagagagaca tacagagaga gagagagaga gagagagaga
     7621 gagagagaga gagagagaga gagagagaga aggggagaga atagtttgga atgtagtctt
     7681 gagcctctgt ttttttttgg ggggggaagg ctaggggtga aattgtgaca gagctcctgc
     7741 tggtggtgac agaccttcag acaatggccc tgggtcatag tttactaact acatgctcaa
     7801 gcagtctgag tgtaaaatac caagtgtgtt cactcctgga ccgtggaagc aactttattt
     7861 agacctgcta ttaagatgag aacataaaat tagggaaggg cctggaaaac atttaaaata
     7921 ttgccaggct tttagtctga aagtacatag gagaggttga gtatttattt atttatttga
     7981 gcatctttta tctattgttt gataatttca tatatgtata tgtctcttga tcatagccac
     8041 accaaagcca ccccccatcc actattccaa gagtgtgggg ctggggcttc tactctcccc
     8101 agacaccgac cagcacctcc cctcctacct ccatgtccgg ccttttcctt ttagatttag
     8161 tttattttat gtacatgggt gttttgcctg catgtgtatt tgttctctac ccccaccccc
     8221 gtgtgtgtgt gagtgtgtgt gtgtgtgtgt gtgtgtgtgt gtaatgcctg tggaggtcag
     8281 aagagggcat agaatcccta gaactgaagt taagaagtat tgtaagccac catgtggctg
     8341 ctgcaaacca aacgtaggtc ctctgcaaga acagggagtg ttcttaactg ctgagccatc
     8401 ttgccaacat cttccttttt tctttctcct tccttccttc cttccttcct tccttccttc
     8461 cttccttcct tcctttcctt cctccctccc tcccttcctc cctccctccc tcccttcctt
     8521 ccttcctttc cttccttccc tccctccctc cctccctccc tccctccctc cctcctttcc
     8581 ttccttcctt ccttccttcc ttccttcctt ccttccttcc ttccttcctt cttcccttcc
     8641 tttcttaaac aactctaatc ctccgagtct agctggtgct gccagctgag tggctgagga
     8701 tcttgttggc ttgactttgt ccaagtcctg caggaaccag tgcagtggcc atgccctgtc
     8761 tagaagacag catttcatag tggtctacct ctggctacta tgttcttttg gctccgtctc
     8821 tcacagtgtg ttctgagcct tgctggagga aggatggata tagatgtcct actaagaact
     8881 gaatattcaa cggtcactta gcctcaggtc tttgacctgt tgagtctgtg ctggtgccaa
     8941 cgcagaaagg ggccttctat ggctaaggct gcgatctacc tggagggcac aaggaggtgt
     9001 tagagcaaga agaaccacac gcgtgagaaa agcaaggggc tgcttcacta aaccctagac
     9061 tatggaatcc acggtaaagc tgaaaagagg cgagattggg cagcatggtg ccatattgct
     9121 ttatacagtg tgagggacac taagggcatg ttgccctcat actctgcaga gctgcaagca
     9181 tctacacagg tgtgagcacc attacatagc tggaccctcc cactcaggag attaaggtaa
     9241 gcatggagtg cccagggcct gtgattcatc agttgacgtt gtcgtcagga gtcagtggca
     9301 ctgtctcgct gtctcggatg tggctttcag gggcctagga ggtccatctc acacagaagg
     9361 gggttttcta tttgctttta aatatactca gacattcaaa gtggccaaca ccaagccctg
     9421 ggatgcttaa cctctcttga cttttatttc atctgcttca aattatgatg atttcatgct
     9481 aatcaatacc tcaagggtat tataaccaag gctctcacgg tgaactctga aacacaaaag
     9541 gaccattatc ctccacacag atcatacaaa agcaactgcc attacccaag gctataatgg
     9601 gaaaggatcg atcactcagt cagcgattac ttttctccct ccctccctcc ctccctccct
     9661 ccctctctcc cttccttcct ccctccctcc ctttctccct ccctccctcc ctctctcctt
     9721 cccttgcata tcttatatcc tagctgaata atttcactgt ttttcacaac caaatgtatt
     9781 tgttgataac aaatggagca aatggaggga tttctttctc tcttcctatt tccattccac
     9841 tgtgtgacag cctaggaact gtgaagcgct cattgtacaa gggaggcaca gtaataaaga
     9901 tgagcagaga gtttccatgc ctgagtaaaa cccaaggcgc ataacgatac cacgatatca
     9961 acaagtttgt acaaaaaagc aggctggcgc cggaaccgaa gttcctattc cgaagttcct
    10021 attctctaga aagtatagga acttcgaacc ctttcccaca ccaccctcca cacttgcccc
    10081 aaacactgcc aactatgtag gaggaagggg ttgggactaa cagaagaacc cgttgtgggg
    10141 aagctgttgg gagggtcact ttatgttctt gcccaaggtc agttgggtgg cctgcttctg
    10201 atgaggtggt cccaaggtct ggggtagaag gtgagaggga caggccacca aggtcagccc
    10261 ccccccccta tcccatagga gccaggtccc tctcctggac aggaagactg aaggggagat
    10321 gccagagact cagtgaagcc tggggtaccc tattggagtc cttcaaggaa acaaacttgg
    10381 cctcaccagg cctcagcctt ggctcctcct gggaactcta ctgcccttgg gatccccttg
    10441 tagttgtggg ttacatagga agggggacgg gattcccctt gactggctag cctactcttt
    10501 tcttcagtct tctccatctc ctctcacctg tctctcgacc ctttccctag gatagacttg
    10561 gaaaaagata aggggagaaa acaaatgcaa acgaggccag aaagattttg gctgggcatt
    10621 ccttccgcta gcttttattg ggatccccta gtttgtgata ggccttttag ctacatctgc
    10681 caatccatct cattttcaca cacacacaca ccactttcct tctggtcagt gggcacatgt
    10741 ccagcctcaa gtttatatca ccacccccaa tgcccaacac ttgtatggcc ttgggcgggt
    10801 catccccccc cccaccccca gtatctgcaa cctcaagcta gcttgggtgc gttggttgtg
    10861 gataagtagc tagactccag caaccagtaa cctctgccct ttctcctcca tgacaaccag
    10921 gctcccaggt cccgaaaacc aaagaagaag aacgcagatc gcagatcgca gatctctaga
    10981 gtcaccgagt tgctttaccg gatgaagagg gccgaaacat actgtccaag gcccttgctg
    11041 gcaatccacc caactgaagc cagacacaaa cagaaaattg tggcaccggt gaaacagact
    11101 ttgaattttg accttctcaa gttggcggga gacgtcgagt ccaaccccgg accggtgcat
    11161 gtggacccca agaaaaagcg gaaggtggac cctaaaaaga aacgcaaagt ggatcctaag
    11221 aaaaaaagaa aggtgcacgt gtccatggtg tccaagggcg aggaactgtt caccggcgtg
    11281 gtgcccatcc tggtggaact ggatggcgac gtgaacggcc acaagttcag cgtgtccggc
    11341 gagggcgaag gcgacgccac atacggaaag ctgaccctga agttcatctg caccaccggc
    11401 aagctgcccg tgccttggcc taccctcgtg accacactga cctacggcgt gcagtgcttc
    11461 agcagatacc ccgaccatat gaagcagcac gacttcttca agagcgccat gcccgagggc
    11521 tacgtgcagg aaagaaccat cttctttaag gacgacggca actacaagac cagggccgaa
    11581 gtgaagttcg agggcgacac cctcgtgaac agaatcgagc tgaagggcat cgacttcaaa
    11641 gaggacggca acatcctggg ccacaagctg gagtacaact acaacagcca caacgtgtac
    11701 atcatggccg acaagcagaa aaacggcatc aaagtgaact tcaagatccg gcacaacatc
    11761 gaggacggct ccgtgcagct ggccgaccac taccagcaga acacccctat cggcgacggc
    11821 cctgtgctgc tgcctgacaa ccactacctg agcacccaga gcgccctgag caaggacccc
    11881 aacgagaaga gggaccacat ggtgctgctg gaattcgtga ccgccgctgg catcaccctg
    11941 ggcatggacg agctgtacaa gcctgcaggc gagggcagag gaagtcttct aacatgcggt
    12001 gacgtggagg agaatcccgg gccgtccaat ttactgaccg tacaccaaaa tttgcctgca
    12061 ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc tgatggacat gttcagggat
    12121 cgccaggcgt tttctgagca tacctggaaa atgcttctgt ccgtttgccg gtcgtgggcg
    12181 gcatggtgca agttgaataa ccggaaatgg tttcccgcag aacctgaaga tgttcgcgat
    12241 tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa ctatccagca acatttgggc
    12301 cagctaaaca tgcttcatcg tcggtctggg ctgccacgac caagtgacag caatgctgtt
    12361 tcactggtta tgcggcggat ccgaaaagaa aacgttgatg ccggtgaacg tgcaaaacag
    12421 gctctagcgt tcgaacgcac tgatttcgac caggttcgtt cactcatgga aaatagcgac
    12481 cgctgccagg atataaggaa tctggcattt ctggggattg cttataacac cctgttacgt
    12541 atagccgaaa ttgccaggat cagggttaaa gatatctcac gtactgacgg tgggagaatg
    12601 ttaatccata ttggcagaac gaaaacgctg gttagcaccg caggtgtaga gaaggcactt
    12661 agcctggggg taactaaact ggtcgagcga tggatttccg tctctggtgt agctgatgat
    12721 ccgaataact acctgttttg ccgggtcaga aaaaatggtg ttgccgcgcc atctgccacc
    12781 agccagctat caactcgcgc cctggaaggg atttttgaag caactcatcg attgatttac
    12841 ggcgctaagg atgactctgg tcagagatac ctggcctggt ctggacacag tgcccgtgtc
    12901 ggagccgcgc gagatatggc ccgcgctgga gtttcaatac cggagatcat gcaagctggt
    12961 ggctggacca atgtaaatat tgtcatgaac tatatccgta acctggatag tgaaacaggg
    13021 gcaatggtgc gcctgctgga agatggcgat ctcgagccat ctgctggaga catgagagct
    13081 gccaaccttt ggccaagccc gctcatgatc aaacgctcta agaagaacag cctggccttg
    13141 tccctgacgg ccgaccagat ggtcagtgcc ttgttggatg ctgagccccc catactctat
    13201 tccgagtatg atcctaccag acccttcagt gaagcttcga tgatgggctt actgaccaac
    13261 ctggcagaca gggagctggt tcacatgatc aactgggcga agagggtgcc aggctttgtg
    13321 gatttgaccc tccatgatca ggtccacctt ctagaatgtg cctggctaga gatcctgatg
    13381 attggtctcg tctggcgctc catggagcac ccagtgaagc tactgtttgc tcctaacttg
    13441 ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg tggagatctt cgacatgctg
    13501 ctggctacat catctcggtt ccgcatgatg aatctgcagg gagaggagtt tgtgtgcctc
    13561 aaatctatta ttttgcttaa ttctggagtg tacacatttc tgtccagcac cctgaagtct
    13621 ctggaagaga aggaccatat ccaccgagtc ctggacaaga tcacagacac tttgatccac
    13681 ctgatggcca aggcaggcct gaccctgcag cagcagcacc agcggctggc ccagctcctc
    13741 ctcatcctct cccacatcag gcacatgagt aacaaaggca tggagcatct gtacagcatg
    13801 aagtgcaaga acgtggtgcc cctctatgac ctgctgctgg aggcggcgga cgcccaccgc
    13861 ctacatgcgc ccactagccg tggaggggca tccgtggagg agacggacca aagccacttg
    13921 gccactgcgg gctctacttc atcgcattcc ttgcaaaagt attacatcac gggggaggca
    13981 gagggtttcc ctgccacagc ttgatgaaga tctgagctct taattaatga tcataatcag
    14041 ccatatcaca tctgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
    14101 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
    14161 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    14221 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gtttaaacta
    14281 actttaaata attggcatta tttaaagtta catatggcgg ccgccctaag gctcgacctc
    14341 gaaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
    14401 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
    14461 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
    14521 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
    14581 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
    14641 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
    14701 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
    14761 ccgaaggtct tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
    14821 gttctcctct tcctcatctc cgggcctttc gacctgcatc catctagatc tcgagcagct
    14881 gaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
    14941 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
    15001 cgatccagat cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
    15061 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
    15121 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
    15181 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
    15241 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
    15301 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
    15361 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
    15421 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
    15481 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
    15541 tgcatcgatg atatcagatc cccgggatgc agaaattgat gatctattaa acaataaaga
    15601 tgtccactaa aatggaagtt tttcctgtca tactttgtta agaagggtga gaacagagta
    15661 cctacatttt gaatggaagg attggagcta cgggggtggg ggtggggtgg gattagataa
    15721 atgcntgctc tttactgaag gctctttact attgctttat gataatgttt catagttgga
    15781 tatcataatt taaacaagca aaaccaaatt aagggccagc tcattcctcc cactcatgat
    15841 ctatagatct atagatctct cgtgggatca ttgtttttct cttgattccc actttgtggt
    15901 tctaagtact gtggtttcca aatgtgtcag tttcatagcc tgaagaacga gatcagcagc
    15961 ctctgttcca catacacttc attctcagta ttgttttgcc aagttctaat tccatcagaa
    16021 gctggtcgag taactttaaa taattggcat tatttaaagt taggcgcgcc ataacttcgt
    16081 atagcataca ttatacgaag ttatgtcgag atatttaaat attagaccca gctttcttgt
    16141 acaaagtggt tgatatctct atagtcgcag taggcggaga gtgctgtggt acctttggtc
    16201 gactatgtgt cctcccaaga ttcaatggca gcccccaagt ccggtgtgat tatgctaagg
    16261 ggtgggcttt aggcatccta taatagggcg ggagagttag ccaggtcctt ttgcccattc
    16321 atgtttctgc caggcatcaa gcagagagag tggctgtcac caataccaat tcatcgtcag
    16381 cttcttgaac acatacattt ctgttgttta tacataaccc tgtcgcagga attctgttac
    16441 agctgaagtg tagtcttcag cagacacaga ttctctggag cgacggggac agcgagggcc
    16501 tggcctgatc agtcattgcc tcttctgatt ccttgcagga gactgttggc ttctggcagc
    16561 cattgcctcc ctcaccttga atgaaaagct gctttatcga gtggttccta gagaccagag
    16621 cttccagaag aactatgctg gcatttttca tttccaggtg tgagtccttg cttgtgacct
    16681 ttgtcactac ttttgtatca tagatattac tccagatctg tggcatcaga aactctggaa
    16741 gcgcagccaa gttactacgt gagattatcc ctctgggtga tttggataca cactccagtt
    16801 tgagaaccat tcctttatac atctatccaa tcatcttcat tctgtctcct tttccctcca
    16861 ggccatgggc caagagaatc actgtggact aaggaccagg agatgggtgt ctgagttacc
    16921 cactctggct ccctgggtga tgtttgagca agctctcttc tccacagact ccaagaaagg
    16981 ctaagtgctt cttggtgtct gtggtatatc ctgtgtgcag atggggcata aagccttctc
    17041 tcggggcatt taaggctcat gaaggttgga gtcttgctca ctgactgcag aaaccgcatc
    17101 caaggatgcc actgaaggac cggctctgct tctacatgtg atccgtgttc tgtttcccac
    17161 ccctagttct ggcagtatgg agagtgggtg gaggtggtca ttgatgaccg gctccccacc
    17221 aagaatggac agctgctctt cctacactcc gaagaaggca atgagttctg gagtgctctg
    17281 ctggagaaag cctatgccaa gtaagtggga aggtaaagac cccggagcct ccctgaaggt
    17341 tctctcccgc tctcctactc ggggagccat ggccaagttt ctccccagca tccagacatc
    17401 cgtaggctat acagaaagca ccactctgaa gagaaatgga aatttgggct aggaattcta
    17461 gatctcatga gatggcgcaa cgcaattaat gataacttcg tatagcatac attatacgaa
    17521 gttatggtct gagctcgcca tcagttcatt tttttaaaga ctctagttct gtctcataag
    17581 aatatctctc tagccattat cttattctct cttaagaccc aaggtaagac cttagggcag
    17641 catagatgtt taaatgatga tgaaaagaaa tgttttgaac taccaaactc aacaaagttt
    17701 ctgttcattc cttgagtgac tttttttttt ttgattgaca ttccaagttt aatgacttct
    17761 ttgggtttct ttggattagt ctggtttgca gtcactctgt tctaaaaccc caggaacttt
    17821 ctggctgtgt tagggcactc cttagcagat acctcccaga cacaagtaag gattctccat
    17881 tcctgtctgt gcctgggcac cccagggacc caagcagtca gggcaaattc tggaagggtt
    17941 acacttggtt ttgaagttgg gaggcctgga ggggctgcct aaggatctgt ttctagtaaa
    18001 atcctggggt atctgtttaa aacagtggtc tgtgcatgtt ctgaggtgtc tgtctgaaat
    18061 ggaagcctgt gtgtgttctg ggctcctctt cacagttggc cagcgtcttt ccctggttct
    18121 ttagtctata tcttggcatt gtccctttcc cacagcccat ggtcccttta ttggggttgc
    18181 tcatgggtgt ggatgatgca tattttcttt cgttttcaac aggctcaatg gttcgtatga
    18241 ggctcttgct ggaggctcca caattgaggg gtttgaggat ttcacgggtg gcatctctga
    18301 gttttatgac ttgaggaagc ctccgggcaa tctgtactac accatccaga aggccctgcg
    18361 caaaggctct ctgctgggct gctccattga tgtaagcctc aactctttgt ctcttttctc
    18421 tgatgacata ttggtttaca atgtacagga aatggtgaat cctggtgagt gggtgggaac
    18481 tagggttggt cacattttaa gattatcatt ggctggtcac tgttggagct gctttagcat
    18541 ggctagactc atcacaggag cacagcccag acccctggat cctaggcccc atctacttac
    18601 agtctttttg ctaagccatc tcagtctttg ggaaaacaaa catggcagtt gacaatattt
    18661 gatgaacttg gagacccctg aatggaagta gaagatccag ctctgacagg aaggttggct
    18721 ctttggaatg ccaatgtggt cttagaaatc agatctggaa gccactaacc tgagggctgg
    18781 ttgttttctc tctggtcatg tgctctcttt tcctgtgtgg gtgtcatcat gggtaggttt
    18841 gtttgtttgt ttgtttgttt gtttgtaggc agcataacta tgatctttca caccacaacc
    18901 tcagcagccc ctcagtgctt gactggctta ttaaaatggg ttttctagaa atgggtccct
    18961 ctgctcatgg gtcatggcca accttacact attcccaccg gagaggtcct tagctgtgtt
    19021 ctatactgtt agaaagcatt gtagtgctga ccccaaagag gtcagggtca gccttactgc
    19081 ttgaactttc ttcttgacac aggtctcaaa cgcagccgaa gcagaagcca ccaccaggca
    19141 aaagctggtt aagggccacg catactctgt gactggagtc gaagaggtag gaaggggtta
    19201 atatgaatga cagccaaagt ctggtgcatc ccaagaagga tgtaggaggt gacactgaga
    19261 aatttcgagt tccctttgag ctttcctgct tgcccagggc aagtttctct ctggatttgg
    19321 agccccggac acatgtgcag ttaactcctg tttgcttctc agaggacagt atctgagcca
    19381 ctctggcctc cagctctctg gttttaatta aattcaactc ttttatgagg tgtggtcaga
    19441 aaagttatag ggactgtcag ttagattctg tatacaaata ttgtaaacaa atattcatgt
    19501 aggcaaatca gtataaaccc gttggagtcc ctaaaagaag taacaacttc tactgcaggg
    19561 ttcagggtgc tatttagtaa tgaaaaggca catatttctc attttcgggc ccaagcattg
    19621 ccaggtacta tacataattt ttattttata tgatttatct atgtccaaat aggatttgca
    19681 taatttgttt tatggttgaa atcatagaca taagtgtacc atcagacaaa ggtaaaatgc
    19741 atccatctaa atggcactac aactgagaag tgaatttact ttaggttttc cagtggtcaa
    19801 tgcagagaga gccacacacc agttctccag cttgctgttt gttgcaacga tgacaacatt
    19861 tactacatag ctctctgttg tgtgtggtat tgtaaattca agtcagctct ataaacatga
    19921 attgagtatg tatgagggaa ctttgggcta gcgtttcatg gaaattatga ggataaagac
    19981 tgatagactg gcatctttat gagtgtatgt gtgcagggtg catgaggacc acactcgaat
    20041 gttcactaaa tagtagggtg agggagtgtc ttagtccatt ttctgttact ataacataat
    20101 ccgtgagtct ggatgattta ttttttaaaa agagaggcgc ttttttttcc atgcatttag
    20161 aggctgggaa gtccaatatg gggtagcttc acactgcttc ctaacagcat caattggaac
    20221 gatgagtggc acagaagaca gacaagctag gcaggctggg tttgctttct atcactcttt
    20281 gcatctaatc cagtcctgaa agaatgagaa ctcacgccac aagaatgaac tcaggctctt
    20341 acgacggaca ttaattccat ttcgtgtttt aatctcttga aagctccacc tgcaaatctc
    20401 atgacagcag tgataaaaac ttaccacagc tgtctaaagg catttataca cttagactat
    20461 ggggattctc tgctgggctt cctgcccttt ctgtgtagag gtgtgagcaa atatctgctc
    20521 acctggatac agcaccagtg acagacagag aaacagtccc acccagatct acctcagtga
    20581 accagtggtt atatcggggt tacttcctag agcccgtacg tctcaagccc agctgcatca
    20641 ccagaaagcc accatggcta gtggtgatat ccctgcatgg atatccctgc agctgcttgt
    20701 gtgatctgga ggcagcactc attgaagaac ctctgtttgc cctgcaactg ttctctactt
    20761 ttataacctc agggaggggc tttgtgaatc ttgtcacttt caggaacttc ctggacctcc
    20821 atgtttcctt tactccccaa gtcttatgca ttttcttcag gaggtgatag ttacacagct
    20881 aagcgatttt gtcgaataac tgacattctc agagtctcat attttttaca tcaaactgtg
    20941 aagcttaaat gcagtgattg tgtatttgaa atgcttagca ttgtacctaa cccagtatta
    21001 agcaataaat ataagttgga ccgagtggtg gtgctggcac atgcccttaa tcccagcact
    21061 tgggaggcag agacagggag atttctatga gttcaaggct agcctggtat acagagtgag
    21121 ttccaggaca accgaggcta catagagaaa tcctatctta gaaaaccatt ttctatatct
    21181 atatctatat ctatatctat atctatatct atatctatat ctatatctat atctatatct
    21241 atatctatat ctatatcatc tatatctttc atggtattga ctttgattat gggatattta
    21301 ttaggttgct gtctttaata ccgagaatga ggtccatctt agtcaactgc aaaatttaaa
    21361 cctaagttta agtagatacc tagttgtttt tgccgtagat tcactcttca cttgttctaa
    21421 taatttgtga aa
//