LOCUS       230498#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20287 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 230498
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000068261
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            10026..10309
                     /db_xref="ENSEMBL:ENSMUSE00000734642"
                     /rank="1"
                     /note="ENSMUSE00000734642"
     misc_feature    4275..10509
                     /note="5 arm"
     misc_feature    10537..10561
                     /note="B1 Gateway"
     misc_feature    10574..10621
                     /note="Frt"
     misc_feature    10622..11121
                     /note="mouse En2 intron"
     primer          complement(10690..10711)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10586..10609)
                     /note="FRT3"
     primer          14787..14805
                     /note="seq primer NF"
     misc_feature    14847..14854
                     /note="RE sites"
     misc_feature    16647..16680
                     /note="loxP"
     misc_feature    complement(14855..14886)
                     /note="Rox"
     misc_feature    complement(16607..16638)
                     /note="Rox"
     misc_feature    14887..14908
                     /note="polylinker"
     CDS             15467..16063
                     /note="puromycin N-acetyl transferase"
     misc_feature    15582..15587
                     /note="Mutated RsrII site"
     intron          11122..11496
                     /note="mouse En2 intron"
     misc_feature    11494..11496
                     /note="Splice Acceptor"
     exon            11497..11550
                     /note="mouse En2 exon"
     primer          11397..11418
                     /note="seq primer FCHK2"
     misc_feature    11551..11673
                     /note="1Dleader"
     misc_feature    11674..11725
                     /note="F2A"
     misc_feature    11821..12537
                     /note="EGFP"
     misc_feature    11731..11820
                     /note="3X NLS"
     misc_feature    14603..14841
                     /note="SV40 polyadenylation site"
     misc_feature    13636..14580
                     /note="ERT2"
     promoter        14919..15430
                     /note="PGK promoter"
     misc_feature    16174..16606
                     /note="PGK pA"
     primer          14661..14684
                     /note="LRPCR RAF5"
     misc_feature    12547..12600
                     /note="T2A"
     misc_feature    12597..12597
                     /note="A to G to kill RsRII site"
     misc_feature    12601..13626
                     /note="Cre"
     misc_feature    11512..11551
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    11512..11527
                     /note="FCHK QC prefix region"
     misc_feature    11528..11545
                     /note="FCHK QC critical region"
     misc_feature    11546..11551
                     /note="FCHK QC suffix region"
     misc_feature    16703..16723
                     /note="B2 Gateway"
     misc_feature    10510..16753
                     /note="Synthetic Cassette"
     exon            16754..17686
                     /db_xref="ENSEMBL:ENSMUSE00000462778"
                     /rank="3"
                     /note="ENSMUSE00000462778 3' fragment"
     misc_feature    16754..20287
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggactggta actcatgaca actttggact ctaagaaata acttacactg
     4321 tgacaagtat cccacagtag aggaatatcc agctgagcat ctgacatgag cccacacaag
     4381 tgtccctccc tcgagaccta aacgaccagg gtaccatggt gtgaaaacac ttcctgatgc
     4441 caagtgcaga cagctcctct ctactgtctg ccttctattt atttctggca ccccaaaccc
     4501 cggcagggtt tcttgtagct caggatggcc ttgtagccaa ggctggcctt gaacttgtaa
     4561 tcctcgtcac tgcctcctgg gtgctgggat tgcaggccta tgccaccatg tctggctctg
     4621 gtaaactctt tcacaaccca cttcctgacg gggtaaggct tgtgaaacct gtgctagggt
     4681 cactgaagac aactgcggcc gaggtgacag gtctgtgggt gtgcagaatg cgaggctgga
     4741 aggataggtg gaacatcaac tgtgcccagt cattctgaaa caaggccgtc tctggctcca
     4801 ctgcgcagat cagggaggca gtggatgctg tctatgccgt gctgacggat cagcacacag
     4861 tgagtgtcac ataaccacaa aacaagcctg gaggtgcagg cctgcgtctt ggtcaggaat
     4921 atcagtcaag agctgccagg gctacatggt ggtgtctcca ctaaagagag aaaaacgaag
     4981 gctggggatg aggcttggtg cgggctcaca cctgcaaccc tggacttagg ctcagggacc
     5041 ggatcagtct ctgagtttgt ggataacctg atctacatac tgagttccag aagagacaag
     5101 cctacagaaa gaatctatgt aaaaacaaac aaacaaacaa acaaaaacaa aacaaacaac
     5161 ctgccccccc ccaaaaaaca aaaaaccaag caaaccagca agccaaaccc taaagaagac
     5221 caaagcaaac cttaaacccc agaccctaac atcagaatga tagcttgttt ttccctcccc
     5281 ccaaaaacgg agagaactat attgcctgcc agtgtccact catgcttgct ctttgcagag
     5341 tggccactgt ttgacgcatg ctttactgat gggtccctct gacagctgaa actgatcttc
     5401 catgacattc acacacacac acacacacac acacacacac acacacaaag cctaaaatgt
     5461 gagcctcttc tggcccccta ctgtgtgtgg taccccccgc tccccagtca tttcagtgtc
     5521 ctagggcagc cctctgacct aacaggagag tggaggggag actcctgcag gttcaatcgc
     5581 tcaatctgct caatggatca atctcattca actatcttca aagcagcggg cagccgctga
     5641 atgagatgaa gttgggatta gagcgccacc tcgtggcaga actggcaggt gccgggaaaa
     5701 cggtctgaaa gaccaggggt tgggagaagt gtggagggat ggtttcctct ttggaccatt
     5761 gcaacctctc aactgtgaca tgcagacaag ttcccgaaac tccccaggga ggcctcactt
     5821 tcgccacggt gtgttaaatg tttcacatgt ttctcagaat tctatacgac agtggcggtg
     5881 cctattccac cattaggaga tcctcagcca agttaactga accgcggaac ttatcattac
     5941 gtagcaaagc tgcagccagt ccgtggctca tgtgttatta caacctccgt ttcggaggcg
     6001 aacaagctcc cgaggtaagg gtgcaggcct caggatccgt aagcggcaac cctaggcacc
     6061 acactgctac cacccaggag ctgcctctta aactgtagcc ggctgctgaa ctctatctag
     6121 gtacacatct cctgaaagca tacctaagaa tactttaagt gctataacaa ccttgggtga
     6181 actgaacggc tataagctct tagagtaaac tggtctctgg tccagccata aattcctgta
     6241 cagtgtgact ctgagctgaa ggttaactgc ctgaccatct aaccgctacc tctctgcttc
     6301 tgtaagcaaa gcttgcttgc tgcagctgtc cagagctgcc tttctgtata cgccacaatt
     6361 gcattttttt tgctcttaaa acctcaaact ggaagtgagg ctgcttccgg cttgctactc
     6421 tgcttggaac agccctactg gtggtatata aagattccac aggacgtatt caggagtctg
     6481 gggacttctt ctcttcaggt atcccataat aaactcagcc aggcagctgc agccttcctc
     6541 tctttaagct taaagaaaat caactacgca ggcccatctt atttgtgtta gaggattttg
     6601 ggtttggttt tggttttgat caaggagagg ccaagctgga gatgagtgag tgagcagggt
     6661 tgttgatctg tgggcagaag gccgtccgtc tcttaacaga cgggcctctc ctccagttct
     6721 agtctggaag ctgctctcca gggaattgct tctgctgtca ctgcaaaggc aacacctacg
     6781 ctgtttccag aactgtggga tttggcaaaa ctgatctaga agtatgccat ctggggctag
     6841 gtcaagaggc tgggggagca ggggcaggag agtgcagggt tctggtagga aacccctgag
     6901 tgttaacctc agcgggataa aactagtcac accatagccg ccatgtaggt taggtgagat
     6961 ctgcttgatg gctttttttt ccttcacttt ttgagactct taggccctgc ccagactggc
     7021 cttgaggtgt tcccgagggc tgggattaca ggagtgtgtt gtgaacttcc tgtctttcta
     7081 ggcggggagg ctgtttctgg gctggctatc tgccagtaag taacacaaat gaactagaaa
     7141 gaggctgtct gcactgtgga gccactaccg atgctgactg gagatgtttt gttgacgtct
     7201 ctctaccgtg gctccggcta gcttggatgt agagcagact ggccttgagt ttgcagagcc
     7261 cggactgcct ccgcctccag accgcaccac cagcttaagt aggatttttg ttacagaatt
     7321 ttggtgagaa tgaagaaaac tggtctggcg cctggtatgt gtcaagggac atgagtccat
     7381 ccctaacaca cagcagcaga tctccggcat aacaccaagt aaaagtttca agtcctctgg
     7441 ccggcccagc tgatcatgtg acaaagcatg gatactttgt aaggtgtttg tggcattatt
     7501 acaaggttgc cattgttttg attatgcaag gagagaagca aaattaaggg ggtgggggtg
     7561 gggtgagtgg agctgggatc tgacctgagc agtcaactgc aaggtaaaaa accatcaaag
     7621 aaacaaagta gtcactgagc tgaaagctag gaaatgtaaa gaaagagttg cgagctatgc
     7681 ggctgggtgt gggagacaca tgcctacaac ctcagcactt gggatgcaga ggccagcctg
     7741 agagactcgg taagaccctg actcaaaaac aaaaacaatc gggctggtga gatggctcag
     7801 tgggtaagag cactgactgc ccttctgaag gtcctgagtt caaatcccag caaccacatg
     7861 gtggctcaca accatctgtc gtgagatctg acgcccactt ctggcacgtc tgaagtcagc
     7921 tacagtgtac ttatttataa taataaatat gggcaggaga gagtgcggtt gccctaaaat
     7981 tcaattccga acaaccagat gaagactcac aactatccgt actgctacag tgtgtactca
     8041 tatacataaa ataaataaat cttcaaacaa acaaacaatc acaccatgct agcacacacc
     8101 ccttagttga ttgggcgatg ttaggataag cttttctgtt actagagaga tatggccaca
     8161 tcccaaccag ggatcttcaa cagttcccaa ggaccaaaga actaacttat ttcccaacta
     8221 ttttgttgta tgatacaggg tctctctgta gtcttggagc tcactgtgta ggctacatac
     8281 ttagagagat tctcctgcct ctgcttgccc agagtcaaaa gacctgcgcc accacaccag
     8341 gcctttattt cccacgcatt ctttcagttc agatctggcc agcttctcaa gaccagttcc
     8401 cacggaccca ctcaccatca gggccctggc gggcagcagc gtgcagcgcc gtgtcgccat
     8461 ggcggtcctg gtgggcaggg tcagcgccga gacgcagcag caggcacagg gcaggggcat
     8521 cgtggcgggc gcaggccctg tggagaggcg ggggctgccc agcgtccaca tcaagcccgg
     8581 gatgtcgctg gaggagagcc tgggctcgca ccagccgtcc tgcagacaag taccgccgga
     8641 agcgtcgctc tcggcggtgc cggcgggaag cggaggccat ggaacaactc tggggctgag
     8701 gaagaagtgg gaaatggcag cggtgaggtc ccagctccgc tcttccccgt ttctcccttt
     8761 acctcctatt tcctccccct tgcatctcca gcaagagata aggcctattc taaactctca
     8821 tcccttagca gatggcaagt ttggggcgga gggtggggat ggggttggag ttgggggctg
     8881 gtggggaggg gggaatcaca gagttaattt ttgatgtaat ttttgagaaa atgatacata
     8941 gttgttttaa ggttccagag cccaagaaaa aagttaattc tgaactttgg agggaaacaa
     9001 caacaacaac aacaaaaatc aagtaaggcc agatgggggc aattttcatt agccacaaat
     9061 ctggagaaaa gtcacaaatt caaagagcga tctagaaatg gaatataatg tacccgacag
     9121 acagacgtag aggcttcttc ctctggggcc tggggggagg ggttactcat cagacctgct
     9181 ccccgccccc ccaaggaccc tcagagacgg aggccggagg cctgggctta agacgctcgg
     9241 agaagggagg tggggaggga ggaggcgcgc caggatgcag gaaatggcgc aagcacacgc
     9301 gcaagtttcg ggcggaaatg aactgtgtgt tccatcccgg aaatcgtacc gaacgctcgg
     9361 actgggtgac ttgtgattag gtctccggag gggaaataag acaatttgcg acatagggtc
     9421 ctcctgcaag cggaagatga gaggcgagct ttcgaaggga ggcagaggca gaggcaggcc
     9481 tggtctatag ggcgagctgc agcgacagcc aggactacag agagaatccc tgtctcgaaa
     9541 cacacacaca caatctcaca cacacacaca taatcacaca cacaccacac aaccacaatg
     9601 aggccaagta tatgggtaga tcaaaaaaga aaaacacatg gttagacact aacaaggcaa
     9661 acgtaggaca gagcagggag atgtgtgcag acgttcagat ttggggggaa agtctgaaga
     9721 gaagatggag gctgaaaaga gggggtaagg gcggagggag gcgtggagac acagttggga
     9781 gtgtaagtga aacccagcgt actgtgtcca agcgcatcct taccttccca caccattctc
     9841 atcgcctttg tgtctccttc gagaccacca accaagatct cctcacaccc aaacactctg
     9901 ccctccgctt cccccagacc catctctgtg gaccctggtg acacggagag gccctgcacc
     9961 tgctgcagta gtcagcacag ggctggtctc tgctgccctc gggcggccgt ttcgggtact
    10021 acctcagtga ggttcgagag gaggaccagt catcagtgtg aggatgacag acacccaccc
    10081 ggtgcaggag actgagtgaa aacgggagcg ctaagaccca gtgggagtgg aggaccgcat
    10141 caagggcccg gaggagtagg cgctggaggg tgggagccgt ctggtacctc ggtcgccttc
    10201 ggaacagcat cctctctagt gataaaaatg gccagtcaga cccagggtat ccagcagctc
    10261 ctccaggctg agaagcgggc agcggagaag gtggccgatg ccaggaagag tgagcccccc
    10321 tttcctctct taggaatatg gaaggagaat ttaggaatag gggatagcgg gcatttgagt
    10381 ccttaggaaa aaaaagccca aggacaatga ggacagatgg gggactgagg ctattagggt
    10441 agaagaaacg ggtgggtttt acagtctagc atttggttgg ttgacaggaa actggataac
    10501 tttctagaga aggcgcataa cgataccacg atatcaacaa gtttgtacaa aaaagcaggc
    10561 tggcgccgga accgaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt
    10621 cgaacccttt cccacaccac cctccacact tgccccaaac actgccaact atgtaggagg
    10681 aaggggttgg gactaacaga agaacccgtt gtggggaagc tgttgggagg gtcactttat
    10741 gttcttgccc aaggtcagtt gggtggcctg cttctgatga ggtggtccca aggtctgggg
    10801 tagaaggtga gagggacagg ccaccaaggt cagccccccc cccctatccc ataggagcca
    10861 ggtccctctc ctggacagga agactgaagg ggagatgcca gagactcagt gaagcctggg
    10921 gtaccctatt ggagtccttc aaggaaacaa acttggcctc accaggcctc agccttggct
    10981 cctcctggga actctactgc ccttgggatc cccttgtagt tgtgggttac ataggaaggg
    11041 ggacgggatt ccccttgact ggctagccta ctcttttctt cagtcttctc catctcctct
    11101 cacctgtctc tcgacccttt ccctaggata gacttggaaa aagataaggg gagaaaacaa
    11161 atgcaaacga ggccagaaag attttggctg ggcattcctt ccgctagctt ttattgggat
    11221 cccctagttt gtgataggcc ttttagctac atctgccaat ccatctcatt ttcacacaca
    11281 cacacaccac tttccttctg gtcagtgggc acatgtccag cctcaagttt atatcaccac
    11341 ccccaatgcc caacacttgt atggccttgg gcgggtcatc ccccccccca cccccagtat
    11401 ctgcaacctc aagctagctt gggtgcgttg gttgtggata agtagctaga ctccagcaac
    11461 cagtaacctc tgccctttct cctccatgac aaccaggctc ccaggtcccg aaaaccaaag
    11521 aagaagaacg cagatcgcag atcgcagatc tctagagtca ccgagttgct ttaccggatg
    11581 aagagggccg aaacatactg tccaaggccc ttgctggcaa tccacccaac tgaagccaga
    11641 cacaaacaga aaattgtggc accggtgaaa cagactttga attttgacct tctcaagttg
    11701 gcgggagacg tcgagtccaa ccccggaccg gtgcatgtgg accccaagaa aaagcggaag
    11761 gtggacccta aaaagaaacg caaagtggat cctaagaaaa aaagaaaggt gcacgtgtcc
    11821 atggtgtcca agggcgagga actgttcacc ggcgtggtgc ccatcctggt ggaactggat
    11881 ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgaaggcga cgccacatac
    11941 ggaaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ttggcctacc
    12001 ctcgtgacca cactgaccta cggcgtgcag tgcttcagca gataccccga ccatatgaag
    12061 cagcacgact tcttcaagag cgccatgccc gagggctacg tgcaggaaag aaccatcttc
    12121 tttaaggacg acggcaacta caagaccagg gccgaagtga agttcgaggg cgacaccctc
    12181 gtgaacagaa tcgagctgaa gggcatcgac ttcaaagagg acggcaacat cctgggccac
    12241 aagctggagt acaactacaa cagccacaac gtgtacatca tggccgacaa gcagaaaaac
    12301 ggcatcaaag tgaacttcaa gatccggcac aacatcgagg acggctccgt gcagctggcc
    12361 gaccactacc agcagaacac ccctatcggc gacggccctg tgctgctgcc tgacaaccac
    12421 tacctgagca cccagagcgc cctgagcaag gaccccaacg agaagaggga ccacatggtg
    12481 ctgctggaat tcgtgaccgc cgctggcatc accctgggca tggacgagct gtacaagcct
    12541 gcaggcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccgggccg
    12601 tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat
    12661 gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc
    12721 tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg
    12781 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc
    12841 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg
    12901 tctgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga
    12961 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat
    13021 ttcgaccagg ttcgttcact catggaaaat agcgaccgct gccaggatat aaggaatctg
    13081 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg
    13141 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa
    13201 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc
    13261 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg
    13321 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg
    13381 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag
    13441 agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc
    13501 gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc
    13561 atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat
    13621 ggcgatctcg agccatctgc tggagacatg agagctgcca acctttggcc aagcccgctc
    13681 atgatcaaac gctctaagaa gaacagcctg gccttgtccc tgacggccga ccagatggtc
    13741 agtgccttgt tggatgctga gccccccata ctctattccg agtatgatcc taccagaccc
    13801 ttcagtgaag cttcgatgat gggcttactg accaacctgg cagacaggga gctggttcac
    13861 atgatcaact gggcgaagag ggtgccaggc tttgtggatt tgaccctcca tgatcaggtc
    13921 caccttctag aatgtgcctg gctagagatc ctgatgattg gtctcgtctg gcgctccatg
    13981 gagcacccag tgaagctact gtttgctcct aacttgctct tggacaggaa ccagggaaaa
    14041 tgtgtagagg gcatggtgga gatcttcgac atgctgctgg ctacatcatc tcggttccgc
    14101 atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat ctattatttt gcttaattct
    14161 ggagtgtaca catttctgtc cagcaccctg aagtctctgg aagagaagga ccatatccac
    14221 cgagtcctgg acaagatcac agacactttg atccacctga tggccaaggc aggcctgacc
    14281 ctgcagcagc agcaccagcg gctggcccag ctcctcctca tcctctccca catcaggcac
    14341 atgagtaaca aaggcatgga gcatctgtac agcatgaagt gcaagaacgt ggtgcccctc
    14401 tatgacctgc tgctggaggc ggcggacgcc caccgcctac atgcgcccac tagccgtgga
    14461 ggggcatccg tggaggagac ggaccaaagc cacttggcca ctgcgggctc tacttcatcg
    14521 cattccttgc aaaagtatta catcacgggg gaggcagagg gtttccctgc cacagcttga
    14581 tgaagatctg agctcttaat taatgatcat aatcagccat atcacatctg tagaggtttt
    14641 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
    14701 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    14761 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    14821 caatgtatct tatcatgtct ggatccgttt aaactaactt taaataattg gcattattta
    14881 aagttacata tggcggccgc cctaaggctc gacctcgaaa ttctaccggg taggggaggc
    14941 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
    15001 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
    15061 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
    15121 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
    15181 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
    15241 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
    15301 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcttccg gaggcccggc
    15361 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
    15421 cctttcgacc tgcatccatc tagatctcga gcagctgaag cttaccatga ccgagtacaa
    15481 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
    15541 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccagatcgcc acatcgagcg
    15601 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
    15661 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
    15721 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
    15781 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
    15841 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
    15901 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
    15961 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
    16021 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
    16081 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgatgatat cagatccccg
    16141 ggatgcagaa attgatgatc tattaaacaa taaagatgtc cactaaaatg gaagtttttc
    16201 ctgtcatact ttgttaagaa gggtgagaac agagtaccta cattttgaat ggaaggattg
    16261 gagctacggg ggtgggggtg gggtgggatt agataaatgc ntgctcttta ctgaaggctc
    16321 tttactattg ctttatgata atgtttcata gttggatatc ataatttaaa caagcaaaac
    16381 caaattaagg gccagctcat tcctcccact catgatctat agatctatag atctctcgtg
    16441 ggatcattgt ttttctcttg attcccactt tgtggttcta agtactgtgg tttccaaatg
    16501 tgtcagtttc atagcctgaa gaacgagatc agcagcctct gttccacata cacttcattc
    16561 tcagtattgt tttgccaagt tctaattcca tcagaagctg gtcgagtaac tttaaataat
    16621 tggcattatt taaagttagg cgcgccataa cttcgtatag catacattat acgaagttat
    16681 gtcgagatat ttaaatatta gacccagctt tcttgtacaa agtggttgat atctctatag
    16741 tcgcagtagg cggttctgtc agctccgtcc acagagaaat atcccaactc aaaaccactt
    16801 gtgtcgcatg cgcagagcct tgggttcaat ccataatatc atccccccca cccaccaaat
    16861 cgcttcacat aataacctgt tgctggaggg gaggcttcta tgtgacagga tccaatattc
    16921 ccccctgaga cttacaagta gcctgttcaa acccgacaaa cccgaatctc tctgttccat
    16981 gacacccact ggaagtttac taatgaatgc ccacgtcatc cccaccggac ccctctgtga
    17041 aatatatgtc ctggtgacag ttatgaaagc accctgactt cagagcaggg gaacctctgt
    17101 tcctctccca cccctggtcc ttgctggtta cacaactggg acaactattt aacctggaag
    17161 cctcagtgtc ctcatctttg tgactggaat gacagcaaca acgctcactt tgtaaggtgg
    17221 ctgctgtgaa ttatctgtga gaattcagca tagttccaga tgacggagtg cctgtatcat
    17281 gaccgcactc tgcgtgcctg ccagaattct aaccccctct cctgactttt ttattccttg
    17341 tgttccagct ttgttcagcc actgtgacac tagtaaccct ctaaagaatt tgtttctctg
    17401 gctctccctt ggcacatcat gcccccgaac agttaaatga taatgttatt tagcccctct
    17461 gtccgagtcc caaatatata tcaaatctct tagcacctct ctgtttctcc tgagctggtc
    17521 tttctcatgg ttggcccccc tctttctctg ttttcccaaa tcttgctgct tccgggagct
    17581 gaagatatct atgacattca catgcatgca ttcctttgtg tgccctcagc accttgcctc
    17641 cttgtgtaac agcggagtgt caataaaata ctggtggatg agtcaattgt tggtgaaagc
    17701 agcctccttt ttgccttgcc gttgctcact ttggttcgct gtcctcagag ttatgcattt
    17761 atatagtttc caaaacactg caaagggctg tcctggaagc tttatggaga aacaaaagga
    17821 cacagtactg tgtcccctct tgaacagaga gttgcataga tagttgtaag ggaaatgttg
    17881 gcagagcagc actctgtctc tttcctggtt ttacactgct catcagcacc agagacagag
    17941 acagacagac aggcaggcag gcagacagac agacagacag acagacagac acacacacac
    18001 acacacacac acacacacac acacacacga gagagagaga gagagagaga gagagagaga
    18061 gagacagaga gacagagact tactatctta ctaagtatct ctgactaccc tggaatttgc
    18121 taggccaggc tagctttgaa ctcacaagag atccacatgc ctctgcctct tgagtactgg
    18181 aattaaagac gtgcaccacc acgcctggct tgacattatg tttgggtatt ggctctcatt
    18241 atgtatctga tgataaaggt cgttgtatcc ccagagccta gatagcactt ggcacacact
    18301 tggcagttga ggaatacttt ggaataaaaa gatgctgggg tggggaagga ggaagttggg
    18361 gtggtggtgg tagggggtga gggggtggga cactgctggt ctctgacccc cattccttta
    18421 tcagttctct tcactgctca tttcaccctg aacttgaatc aatgaggtaa ttcatagtct
    18481 tatatgactt ttctgtagag agacattaaa aaaaaaacta taagccaggt gtggtggcgc
    18541 atgcctttaa tcccagcact tgggaggcag aggcaggtgg atttctgagt tcaaggccag
    18601 cctggtctac agagtgaatt ccaggacagc cagggctaca cagagaagcc ctgtctcagg
    18661 aaaaaaaaaa aaaaatctat gccaaataag gcagtcatga aacaccaaag atatctcacc
    18721 acccaagtct cacttggcag accaaagagc tcgctggagt tattggcagt atggtgggtg
    18781 ataggttact tataggagtg agactggagt agcttcccat cagaaagtcg cccaccagcg
    18841 tgacttgtat gcagaccaaa gagtcctgcc accaggagct gtttattggt gacacaatct
    18901 gggagaagtg ttccgtgtct tgcaactttt tttctgaatt tcctgagcct tctccactct
    18961 catcaggagg aaatgtttcg ggggaaatag ctatacaaaa gcaaggagat ttttgaagag
    19021 gagggacaca tgtgatagga aattggggtg cttacaccct ggtgtttccc tgctagctgt
    19081 attgccttgg ccaccttgtt ttatttctct attttcatat gtttacagga agagtggagc
    19141 ggggcatggg tctgcattca aatcctccag caggagaatt gagagcttga ggccagtcta
    19201 tataactgct caaactcagt aacagagaaa ggaagaaaaa aatggcaaga cagatgaggg
    19261 gcactcacct tgctgtggcc ccagtggccc aggttttaag cattgttaaa atcacagcca
    19321 gctgttaaga caatcccttc ctgccctggc taccttaacg aggactgtca gcatggccat
    19381 tttgaatctt ctataaagtc tttgaggaaa ccacacagct aatggctcag ccaggattgt
    19441 ttctgtcctc tctctcctcc cccagtggag ggtggaactt cccatagtcc agtagcagga
    19501 agatgtgtta tcactgatgc caaagggaca atattatgat tggtgcaaac acaagtcttc
    19561 atttgcacga gtttgctgtt tgctgtgttt ggtgcaaaca ccctttgctg gctgtaaagc
    19621 agaaagtttg gctcacttgg gtcagctggg acaaacctta ctatgctacc ctccacaatg
    19681 tcacattagg tccagtaaca ggaagacagt ggctaagagc ccgccaacta ggtcactagg
    19741 agagttcctg gaaacgcttc aaagctaaag gcccaggtac cgcactttct ggagctacag
    19801 ttcgtaataa gttaccaggg ccgaggaatt cccactcctc tctgatttat acaaaacccg
    19861 ccggcctgct gcttagggct gccaaatgcg gagggatcaa aagctaccaa gccccagccc
    19921 agagagctta tgcgttgagc aggacacagc cagctggttg gcaacactga tacttagtgt
    19981 tactttgtat taatattaag tgtccttaat agggaaagcc cgagcttgtg tttatgtagc
    20041 aaggcaacag gacaacactt ctgctatatg taacatctca aagggaatac cacatggggg
    20101 gagggggatg tccaggctga ataatggaac tgagattatt ctgctgctct agttagcaag
    20161 aattatggaa aggtgcataa cctcaaaccc caccatttat ttagcaccta gacagaatgt
    20221 aagcctccat ctttcgatat aattttgggt aaatcctgcc tattctctgc acgactgtaa
    20281 tgggcgt
//