LOCUS       40485#pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21236 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 40485
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000105461
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..9139
                     /note="5 arm"
     misc_feature    9167..9191
                     /note="B1 Gateway"
     misc_feature    9204..9251
                     /note="Frt"
     misc_feature    9252..9751
                     /note="mouse En2 intron"
     primer          complement(9320..9341)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9216..9239)
                     /note="FRT3"
     primer          13403..13421
                     /note="seq primer NF"
     misc_feature    13463..13470
                     /note="RE sites"
     misc_feature    15263..15296
                     /note="loxP"
     misc_feature    complement(13471..13502)
                     /note="Rox"
     misc_feature    complement(15223..15254)
                     /note="Rox"
     misc_feature    13503..13524
                     /note="polylinker"
     CDS             14083..14679
                     /note="puromycin N-acetyl transferase"
     misc_feature    14198..14203
                     /note="Mutated RsrII site"
     intron          9752..10126
                     /note="mouse En2 intron"
     misc_feature    10124..10126
                     /note="Splice Acceptor"
     exon            10127..10166
                     /note="mouse En2 exon"
     primer          10027..10048
                     /note="seq primer FCHK2"
     misc_feature    10167..10289
                     /note="1Dleader"
     misc_feature    10290..10341
                     /note="F2A"
     misc_feature    10437..11153
                     /note="EGFP"
     misc_feature    10347..10436
                     /note="3X NLS"
     misc_feature    13219..13457
                     /note="SV40 polyadenylation site"
     misc_feature    12252..13196
                     /note="ERT2"
     promoter        13535..14046
                     /note="PGK promoter"
     misc_feature    14790..15222
                     /note="PGK pA"
     primer          13277..13300
                     /note="LRPCR RAF5"
     misc_feature    11163..11216
                     /note="T2A"
     misc_feature    11213..11213
                     /note=" A to G to kill RsRII site"
     misc_feature    11217..12242
                     /note="Cre"
     misc_feature    10142..10167
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    10142..10157
                     /note="FCHK QC prefix region"
     misc_feature    10158..10161
                     /note="FCHK QC critical region"
     misc_feature    10162..10167
                     /note="FCHK QC suffix region"
     misc_feature    15319..15339
                     /note="B2 Gateway"
     misc_feature    9140..15369
                     /note="Synthetic Cassette"
     exon            15789..15867
                     /db_xref="ENSEMBL:ENSMUSE00001304522"
                     /type="targeted"
                     /rank="10"
                     /note="target exon 1 ENSMUSE00001304522"
     misc_feature    15370..16031
                     /note="Critical Region"
     misc_feature    16055..16088
                     /note="Downstream LoxP"
     misc_feature    16032..16111
                     /note="synthetic loxP region"
     misc_feature    16112..21236
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatatttg aaccgagcat cctctgagtc tccataacga ttcctttgta
     4321 acttgcacag gcatttacat caggtatcag agcaccccca cccccacccc acccccaccc
     4381 cgtgcactca ttcagtaatt cagcctcata cccatctccc agagagttat taggtgccct
     4441 gtatatactg tccagggcta agtatgctac acccttcttt tctagagaat tccatggagc
     4501 agaccatttg cgagtgagtc tttcttcgca tttggtcatc tcatgacccc tctgttccat
     4561 ttcctatgac ttccttccct tagctcagag accttagcat atcgttgtgt attcatgcta
     4621 cccatggtct ggcaagttcc tgagaagcta ctgcgtccat gctctggggg cagggtgagc
     4681 gcttttacaa ggggtgcagc ccagctgagg aagacagatg aagcggtgag ttgggtcccg
     4741 tctcttctaa gcactgacca gccccatagt cccattgtgc atctgcttct cttagctccc
     4801 ccactggaaa agggatcccc acttccccat cccctccaag ccaatcccca caagctccag
     4861 gtcccatgtc tccaggctgc taatcagttt ccatgccttg atctggatct ccgtgtccct
     4921 ttagtgtgac gcgctgattt cgcacactcg ttccctagct gggtctgggc tgagatgcag
     4981 catggcctgt tccataaccc taggatagga ggacccaggc atccctcatg cccagccctg
     5041 tctccgaggc tcctagggtc cctgtcaaga ccctgtcccg ccctcctggc tccccccccc
     5101 ctttatctgt ggctcccagc atgtatactt ccctctatca gtgtatcagg ctagcaagct
     5161 tcatctaaag tgtatatatt acctaataaa atccttctta tttgggatta ttgcatcaaa
     5221 ttttaatttc ctcactcgaa cagcagcatg tcagcctgcc cgtcagcgag gggccgacag
     5281 aaggtgaaag gcgaggacag gctgcacaaa ttactgccgg cgatgcctat cagccctttg
     5341 actggaattg actggaatta aggactgaga aataggattt tctgcctcag aatctgctgt
     5401 ggcagctctc gttggaagtc cccagtagcc cccaccagcc agcccccacc ctagtaatgc
     5461 tccccccacc cctcacacct ccaaagcaaa ctgcatgtca cctgtcacct ttcaagtcct
     5521 taatcgtggc agggaaatgg gggctggggt gtcccagcta agcttatacg gaaggcaatg
     5581 gggagtagtc cctgaggtcc aggacagtcc agtttggtcc agaaacatgg tttcttctag
     5641 tgaagtcccg ggttgtgccc cacttcctgc cctccaggca gagtcctggt cctccagtct
     5701 ctgttggaat ggcaggcttg ttccaggttc aaccttccat tccccagtta caagcaccct
     5761 gcttacccac cataccttgt gagcttcagc aaactcccga aagctccaag aatgtgtcct
     5821 ccttgcaggg ggaggggtta ggagcatctc tgcataccca tcatgcccag cgcacggtta
     5881 gcacccaggg gacgctcact taaccggctc ttccccatcg ggtcccagct gctcaagggt
     5941 agacaaagcc ccttagcttg ggagtggaac ctatcagagt gggatgttca gtatccgaac
     6001 tagttgaagg tgtttagaaa tgtatgcata ggggctggag agatggctca gtggttaaga
     6061 gcactgactg cacttccaga ggtcctgagt tcaaatccca gcaaccacat ggtggctcac
     6121 aaccatttgt aatgagatct gatgccctct tctggtgtgt ctgaagacag cgacagtgta
     6181 ctcacaaaca tacaataaat gcatctcttt ttttgaaaga aagaaaagaa aagaaaagaa
     6241 aagaaaagaa aagaaaagaa aagagagaaa gaaagaagtg tatgcatagt cccagacaca
     6301 gcactaaagt ttcacgtgat catgcagcaa gtctagggcc caactttata gtgtcagtta
     6361 tatttcctgt gtatggggtg gggggaagag ggagtgtgtg tgtgtgtgag agagagagag
     6421 agagatagag agacagacag agagagacag agagagagac agagacagag agagagacag
     6481 agacagagag agagcaagca ggagagtgag tgtgtgtatg tgtgtgtgtt catatgcatg
     6541 agtctatata tcagttgctg gacagtagag cctgttatac ccatttcaca gatagaaaaa
     6601 caacaactga gaagtccaga cttacaggaa tcaaccccct aactgcctct ctgcttctct
     6661 tcctgtcaca aggagtcagg cccttgtagg cctatgcacc tgtcacctct ttgagcagag
     6721 ccttgtttcc cagaaggtca cctcttcccc acaacatgag tgcctgagga aggcaagact
     6781 cccttagcag gggtccgtct tcagccctct agctggagca gggtctccct ctccctctcc
     6841 tggccgtgcc cctgggagcc tcacttccca cagagccaca cgctgcaagc gtggactcat
     6901 ctttggaggc ccggctctgt gccagcaggc tgcacttcct cctcgttcct tgtctttgga
     6961 aataagttgc agtatattga aaatataatc actgagactc tgaaacaaga gccatgggct
     7021 cccaggagcc cccacacctc cttgttaatt ccttctcata acgttttcag gccttctgtc
     7081 tgcacttgta ttaaaagctc agagttttat taaaagtgga tttatttacc caaaaagaga
     7141 tgaaacccca aacctgattg ggagcctggc agagcttgcc cggcagaggc tcggctccaa
     7201 ctatggctgg gagagggggc cccttatcct cgtgtcccac agagccagct agcgtctgct
     7261 gagttccccc gacacagcta tggatgagtg ggggatgggg tctacagggc tccgaggact
     7321 gagggctctt aggccataga cttctctgga cagcccagta tagctgcctt agcctgtccc
     7381 cttgagccca gcacaaccta cctgggatat ggaaaagcat agatctgtcc cttcagtgta
     7441 ttgtttagta attcagcctt agaactttag tgatctccgt gctcaggcat cctgataggt
     7501 gggtccctgc tccagcagcc caggggcaac tcaggaaccc tctagtagga cgcatgtagg
     7561 cacccaggca gaatggaact acccctgttt ctagaaagcc tgttctggaa gctaaaacct
     7621 tgggatagtt gtatacccta ctggaagtca gcccaggtct tccctgatgg atagcgaact
     7681 cttagatgag cagctatccc agaagagcct ccagcagggt gatggaggaa agcagtgtag
     7741 tccctttaag tcgagagctg tgcttccctg tggcttccat ggaccggagt caggggctgt
     7801 ccatgctccc tctgtgagag gaagtttggg aggacaaggt gactggtctc ccatgtgact
     7861 acccttggcc cctgctcagt ctagaacaca tggcatctcc agggatggct ggcaagtagg
     7921 catttccaaa ttccacggga accagtgctt agaatagttc ctgccacaga gcagtggcat
     7981 taggaagtga cattcgcatt attatagcag aaccctgcgg cggccgcggc acctggtgga
     8041 caggaacatc agacccttag catttaaatg tgttcctggc ttcctgttct ttcactctct
     8101 gctttgggac ctccagcagt ctctcagcca tctgcttctg tttcttcatc tgcaggaagg
     8161 ggctaggtct taggacactg gcaggctggg gtacctgaga acatcgggcc agcgtttgag
     8221 cagttctctg tgccaggaaa gatgtgcttt acagtgtggt cagttggttc tttacatggc
     8281 ctatttatcc agcttcttaa caagcccaga ggatccaacc aggatccctg gaggggagga
     8341 caaagaagag gccaggacgg cttgtcatat ggcctggtct ctgcatatat cccgggatat
     8401 tcccacgttc cctcagggag ctttgggtca ttccgtcttc tgcattctag gctaagggag
     8461 ggtgaaaggg cttctggaaa gagacatgca gtctgggaaa ggggtttgtg gctcctgttg
     8521 tgactccaca gccagactgg gaggacttgg agagatgttg ttggccctga gacaagagaa
     8581 ctgtctgtcc aaaggtatgt gagaagcaga caagacctcg tgagcccttt atacacttgt
     8641 gagtgctagg atactttcat cacccccgcc aaagacggag acagggccag tctgccgtcc
     8701 tttgcccagg ggaaactact agggcatagt ccccaacctt cctagtgcca caaccttgta
     8761 atacagttcc tcgtatggtg gtgacacccc agccataaaa ttatttttat tgctacttca
     8821 taactgtttg ttactgttct gactcgtaat gtaaatatct gtgccctctg atggtctagg
     8881 aaacccctgt gaccccatgt caagacccac aggttgagaa ccactgtgct aggacctgaa
     8941 cctcaggtgt aaggcgagca gggatcgctg acatatgcag tggtgagatg tcacgcagcc
     9001 tggggagcaa ggtagaagtt gctcttagca aaggacatct tagcaagtgg tggtcctggt
     9061 ggtgatgcca ttgttttcca tagatacgtt tgtttattca ttcaacaaac tgccatcctc
     9121 tgatggttac ttaccagcaa aggcgcataa cgataccacg atatcaacaa gtttgtacaa
     9181 aaaagcaggc tggcgccgga accgaagttc ctattccgaa gttcctattc tctagaaagt
     9241 ataggaactt cgaacccttt cccacaccac cctccacact tgccccaaac actgccaact
     9301 atgtaggagg aaggggttgg gactaacaga agaacccgtt gtggggaagc tgttgggagg
     9361 gtcactttat gttcttgccc aaggtcagtt gggtggcctg cttctgatga ggtggtccca
     9421 aggtctgggg tagaaggtga gagggacagg ccaccaaggt cagccccccc cccctatccc
     9481 ataggagcca ggtccctctc ctggacagga agactgaagg ggagatgcca gagactcagt
     9541 gaagcctggg gtaccctatt ggagtccttc aaggaaacaa acttggcctc accaggcctc
     9601 agccttggct cctcctggga actctactgc ccttgggatc cccttgtagt tgtgggttac
     9661 ataggaaggg ggacgggatt ccccttgact ggctagccta ctcttttctt cagtcttctc
     9721 catctcctct cacctgtctc tcgacccttt ccctaggata gacttggaaa aagataaggg
     9781 gagaaaacaa atgcaaacga ggccagaaag attttggctg ggcattcctt ccgctagctt
     9841 ttattgggat cccctagttt gtgataggcc ttttagctac atctgccaat ccatctcatt
     9901 ttcacacaca cacacaccac tttccttctg gtcagtgggc acatgtccag cctcaagttt
     9961 atatcaccac ccccaatgcc caacacttgt atggccttgg gcgggtcatc ccccccccca
    10021 cccccagtat ctgcaacctc aagctagctt gggtgcgttg gttgtggata agtagctaga
    10081 ctccagcaac cagtaacctc tgccctttct cctccatgac aaccaggctc ccaggtcccg
    10141 aaaaccaaag aagaagaacg cagatctcta gagtcaccga gttgctttac cggatgaaga
    10201 gggccgaaac atactgtcca aggcccttgc tggcaatcca cccaactgaa gccagacaca
    10261 aacagaaaat tgtggcaccg gtgaaacaga ctttgaattt tgaccttctc aagttggcgg
    10321 gagacgtcga gtccaacccc ggaccggtgc atgtggaccc caagaaaaag cggaaggtgg
    10381 accctaaaaa gaaacgcaaa gtggatccta agaaaaaaag aaaggtgcac gtgtccatgg
    10441 tgtccaaggg cgaggaactg ttcaccggcg tggtgcccat cctggtggaa ctggatggcg
    10501 acgtgaacgg ccacaagttc agcgtgtccg gcgagggcga aggcgacgcc acatacggaa
    10561 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccttgg cctaccctcg
    10621 tgaccacact gacctacggc gtgcagtgct tcagcagata ccccgaccat atgaagcagc
    10681 acgacttctt caagagcgcc atgcccgagg gctacgtgca ggaaagaacc atcttcttta
    10741 aggacgacgg caactacaag accagggccg aagtgaagtt cgagggcgac accctcgtga
    10801 acagaatcga gctgaagggc atcgacttca aagaggacgg caacatcctg ggccacaagc
    10861 tggagtacaa ctacaacagc cacaacgtgt acatcatggc cgacaagcag aaaaacggca
    10921 tcaaagtgaa cttcaagatc cggcacaaca tcgaggacgg ctccgtgcag ctggccgacc
    10981 actaccagca gaacacccct atcggcgacg gccctgtgct gctgcctgac aaccactacc
    11041 tgagcaccca gagcgccctg agcaaggacc ccaacgagaa gagggaccac atggtgctgc
    11101 tggaattcgt gaccgccgct ggcatcaccc tgggcatgga cgagctgtac aagcctgcag
    11161 gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc gggccgtcca
    11221 atttactgac cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg
    11281 ttcgcaagaa cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga
    11341 aaatgcttct gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat
    11401 ggtttcccgc agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc
    11461 tggcagtaaa aactatccag caacatttgg gccagctaaa catgcttcat cgtcggtctg
    11521 ggctgccacg accaagtgac agcaatgctg tttcactggt tatgcggcgg atccgaaaag
    11581 aaaacgttga tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg
    11641 accaggttcg ttcactcatg gaaaatagcg accgctgcca ggatataagg aatctggcat
    11701 ttctggggat tgcttataac accctgttac gtatagccga aattgccagg atcagggtta
    11761 aagatatctc acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc
    11821 tggttagcac cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc
    11881 gatggatttc cgtctctggt gtagctgatg atccgaataa ctacctgttt tgccgggtca
    11941 gaaaaaatgg tgttgccgcg ccatctgcca ccagccagct atcaactcgc gccctggaag
    12001 ggatttttga agcaactcat cgattgattt acggcgctaa ggatgactct ggtcagagat
    12061 acctggcctg gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg
    12121 gagtttcaat accggagatc atgcaagctg gtggctggac caatgtaaat attgtcatga
    12181 actatatccg taacctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg
    12241 atctcgagcc atctgctgga gacatgagag ctgccaacct ttggccaagc ccgctcatga
    12301 tcaaacgctc taagaagaac agcctggcct tgtccctgac ggccgaccag atggtcagtg
    12361 ccttgttgga tgctgagccc cccatactct attccgagta tgatcctacc agacccttca
    12421 gtgaagcttc gatgatgggc ttactgacca acctggcaga cagggagctg gttcacatga
    12481 tcaactgggc gaagagggtg ccaggctttg tggatttgac cctccatgat caggtccacc
    12541 ttctagaatg tgcctggcta gagatcctga tgattggtct cgtctggcgc tccatggagc
    12601 acccagtgaa gctactgttt gctcctaact tgctcttgga caggaaccag ggaaaatgtg
    12661 tagagggcat ggtggagatc ttcgacatgc tgctggctac atcatctcgg ttccgcatga
    12721 tgaatctgca gggagaggag tttgtgtgcc tcaaatctat tattttgctt aattctggag
    12781 tgtacacatt tctgtccagc accctgaagt ctctggaaga gaaggaccat atccaccgag
    12841 tcctggacaa gatcacagac actttgatcc acctgatggc caaggcaggc ctgaccctgc
    12901 agcagcagca ccagcggctg gcccagctcc tcctcatcct ctcccacatc aggcacatga
    12961 gtaacaaagg catggagcat ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg
    13021 acctgctgct ggaggcggcg gacgcccacc gcctacatgc gcccactagc cgtggagggg
    13081 catccgtgga ggagacggac caaagccact tggccactgc gggctctact tcatcgcatt
    13141 ccttgcaaaa gtattacatc acgggggagg cagagggttt ccctgccaca gcttgatgaa
    13201 gatctgagct cttaattaat gatcataatc agccatatca catctgtaga ggttttactt
    13261 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
    13321 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    13381 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    13441 gtatcttatc atgtctggat ccgtttaaac taactttaaa taattggcat tatttaaagt
    13501 tacatatggc ggccgcccta aggctcgacc tcgaaattct accgggtagg ggaggcgctt
    13561 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
    13621 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
    13681 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
    13741 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
    13801 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
    13861 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
    13921 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cttccggagg cccggcattc
    13981 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
    14041 tcgacctgca tccatctaga tctcgagcag ctgaagctta ccatgaccga gtacaagccc
    14101 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
    14161 ttcgccgact accccgccac gcgccacacc gtcgatccag atcgccacat cgagcgggtc
    14221 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
    14281 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
    14341 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
    14401 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
    14461 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
    14521 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
    14581 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
    14641 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
    14701 cagcgcccga ccgaaaggag cgcacgaccc catgcatcga tgatatcaga tccccgggat
    14761 gcagaaattg atgatctatt aaacaataaa gatgtccact aaaatggaag tttttcctgt
    14821 catactttgt taagaagggt gagaacagag tacctacatt ttgaatggaa ggattggagc
    14881 tacgggggtg ggggtggggt gggattagat aaatgcntgc tctttactga aggctcttta
    14941 ctattgcttt atgataatgt ttcatagttg gatatcataa tttaaacaag caaaaccaaa
    15001 ttaagggcca gctcattcct cccactcatg atctatagat ctatagatct ctcgtgggat
    15061 cattgttttt ctcttgattc ccactttgtg gttctaagta ctgtggtttc caaatgtgtc
    15121 agtttcatag cctgaagaac gagatcagca gcctctgttc cacatacact tcattctcag
    15181 tattgttttg ccaagttcta attccatcag aagctggtcg agtaacttta aataattggc
    15241 attatttaaa gttaggcgcg ccataacttc gtatagcata cattatacga agttatgtcg
    15301 agatatttaa atattagacc cagctttctt gtacaaagtg gttgatatct ctatagtcgc
    15361 agtaggcggc ttccctttta tttgctctcc ttggaaaact cctactaact cctcacaatc
    15421 catcctaaat ggtaccatct gcatgcccct ttcgaggctt ctccaggtgg gctttggata
    15481 cccactctgt cctgtttatg tctccatctc atctgcccca gatgtgtgcg gcagctgtct
    15541 gtgtctgtcc ttgaccccct tgctcgagtc catacttgac tatctgtgtc gtggaggaat
    15601 ggcaggtgct aagcgcaagg ccattgtgag tcaggagcaa agctgcatgg tatacacact
    15661 tcccaggcaa gcctcgaagt tccgaattag gctagggagg gacagtgggg tgctaggaca
    15721 cccataaaga aagatagcta cctggcatct aggtgactga acacaagtcc cttctctctc
    15781 caaaccaggg cacaactgtg cgggtgatca ctgctgtgga ccaggataag ggacggcccc
    15841 gtgggattgg ctacactatt gtctcaggta agccaaagcc tgcgttgttt gctggataga
    15901 ctaagagagc gcctgttggc ggctctggct caaagagatt gaatggcatt tgagacctgc
    15961 ctccagggac aatccttgct aaggaagggg acaaatggat ataaaatgga ggaactgggt
    16021 taaaaggcca tgagatggcg caacgcaatt aatgataact tcgtatagca tacattatac
    16081 gaagttatgg tctgagctcg ccatcagttc atacctaact tcttacaggg ctccagggtg
    16141 tctgttctta tctgctgaaa aggtggcaat ttttacatca ctttggaaca actagtattg
    16201 ctgctgtctg atttcacgtt ctagtcacac ttcaatctga taggcagcac cgggatatca
    16261 ccgtgggagg caccttcgcc tcttcctacc caaagatccc ttggccgtgc agaacatgac
    16321 tgactgaact gatgtggagg agtaaggcaa tgcctaactt gtgcctcacc ccatagatgg
    16381 acccatggcg gttctgagag gctgtccagg gactaagctg cccccactta gaaacgctgg
    16441 ttctgctcct gcatcacacg tgcctgctca cagcatctct cgctcagtgt acggtctctg
    16501 aagggccagc ctggtgtatt caggcaagcc agacagatga ggaagtcaga gtgtttccca
    16561 aaaggggaga gaaaggcata ggcatcaagg gagacgggaa ggtctcctga gccggctgtg
    16621 ttggctaggt ctctcacttg tcagttgtgt attgggaaca ggaagaatgc ttgcgtcaag
    16681 ggggtttgtg ggtcacatgg cttactcctg tgaagagcta atgcgtgaac actttgtatg
    16741 tatctgctct tgataatacc accagcagcg gtttggagag ccccttgctt ctctagggtc
    16801 acaggtagcc aggagtgagt gttatgggcc ttgggcaacc tatggttaca tacaagactc
    16861 ctattatact gtgatcccag agagccccag ggcagccagt ctgcatggaa catctgtaga
    16921 aattggcact tgatcccctg aatcccttgg atgcctccta ccatgtccag gatcctttct
    16981 gatgtccagg tcaggatcca gcccagcaca gaccttgatg tacactcaca gaatgattgt
    17041 cattctcggg tcccacccgg ggatggggcc cactggtgtg gtgacctgag cctcaggcac
    17101 ctaggcagtc tgggctggtg ataggggctc tgtctttatg tggcaaggcc cacctgcccc
    17161 agtcaggggc acagagcccc aagctaagaa taggcacctg cagttaacct gtctgaatct
    17221 gaggtcctgt cccctctggg ctgtttgtca gccacaagcg aggggattta tatctgccca
    17281 cgagtgctcg ccccactact aatgatcctt ctgttttgcc aagagcaaag aggccttcag
    17341 tggtaagtta tggctccatc tctatcaatt tgttctcagc cccgcgcggc cgcacggagc
    17401 ctggtgaatg gcaagcttct ttcccgccac catgggcacc agtagcatcg ccctctccac
    17461 agcaggctgc aaggaggctc ctgtcccagc ctgaattctg agtggccatt ccctagcctc
    17521 atcctcctct tagcacaaac agtcctactt caaggaacgc tctgcctacc ccctgtagag
    17581 cgacgtgggt gtgagggctt tgctccctgc cagggctctt tccgaaagat gcttgacctg
    17641 ctgcaggctt gatggtgctt acctataaga caaggttaaa aatcctgggg ttgtaggtgt
    17701 aattcagcgg ccaaatgctt gcttaacatt ctggtgctat gtaaaaccgg ccatggcggt
    17761 gcccacctgt aaaaccagtg ggcacttggc tggtggagga ggaaggatca gaaaggttga
    17821 ggacatcatt aggtgagttt gaggtttgcc tgggctacat gatgccctgt tgcaaaaata
    17881 aaataaaata aataaaacat aaaaaaaaat aaatggaggg aaggggaaaa cacaaataaa
    17941 ataaaatgta gaaaaggaga agagaatccc tacgtgtccc gatggctctg aatgaccata
    18001 aaacactaag gattgactag taattgtcag agagggatcc ttgaagctga ttcatttcct
    18061 caaggtatgg aaaagcattg agcaaggacc accatccttc atgcgcctcc aggcagactg
    18121 cctagtacag cagcccccaa ggccatagag cacttgtccc ctggcctttc tcaatggaga
    18181 aggacagtcc gtgtggcaca taggactggg gcatgactcg gtaagagccc acaggcttag
    18241 cttatgtgag gctccaggtt ctatgtgggc acgggcaaga aacagtatca gtgcatcccg
    18301 ttggttatgt gctaagatgg tagcatttcc cttcttctgg cttgcccaca gtccagcatc
    18361 aaactcagtg ttcagtctta gatttaagtt gcagctcggc tcagattgct atagaagacc
    18421 ctgctctagt gtgagccttc cctggactta gttccagatg tgggccatgc ctggcagggt
    18481 actactgtga ggaccaagca agccaagcaa accagcagtt ggtcagtcag tggagcatgg
    18541 attccacagc aggtgctcac agaaacagga agcactctcc cttcacccac cacagttggc
    18601 taagggctgt ctaggtcagg aatatgatgc cctgggaggg cagggaccag gtaccatctc
    18661 cattacaaga acctgtactt ttgccaggaa ggtcgacttg agctgtgcag ggactgcaaa
    18721 gatgtccggg gtaactacgc aggatgcatg ggcagtgagg gggagggaag aggctgaggt
    18781 gcatctttac agtggggcta cataccagca tgagctgggg ctccccggga acccccctca
    18841 actttccttt gtaagaagaa acaagaaaat tctgtgagac actgcacacc caaccgttcg
    18901 gagtgggaac cttgctaagg tttggccgtc aaagctggca tgcatgggaa tcacgaatgg
    18961 ggattcacag aatgggcaaa gtcatgggag tctgacacat ctgtcactta cagtgactct
    19021 gtggccgagc aaagagcacc tgaggttgtg aggagacaga ctactgaagg taacaggatc
    19081 agctatgcga tgtgtggatg gccagtctgg gtcctgagag cagtggaagg ccattgtgtc
    19141 ttcaacagaa gaacaaagcc atgtctgagc tcggggtgct cagtaagtcg agagagtggg
    19201 gccagtggtt tggagaagag caggctgaag caagcagacc agcaggaagg atgctataat
    19261 gccccaccca gaggtggtgg cagccttagc cagcagtcaa ggcacaagga gagaacagag
    19321 aatgagagga gggccaggaa gctcccagga cccagcaact gatacccagg gggcctcagc
    19381 caaggaggag tctgattgct gtacaagcat tcatgctttc ctttcttctc agtgatgagg
    19441 gtgggaccca ggtccttgca tatgctaggc aggtgctcta ccactaaacc catactccat
    19501 atgcccagct ccctcacctt ggaggcctgg gcagaggaac ttcacagttc aatgccagat
    19561 gctacttgca cccaagagac acctgtctcc cctggccagg tgagaagtaa gtcactcctt
    19621 catagctgtg aaaatcaaag agccccgtgt ttactgtgct ggcaataata agtcaaatcc
    19681 ccaaagacaa actccacatg gtgtgcctta tgtgtggaat cctgacgaag aagaacttgt
    19741 agtgacagag aggaaaaggc tattccctga ggtggtgagg tggggggacg ggaagatcct
    19801 gctaagaggg tcattcatca gagtaagtcc tggggactgt cctctgcagt ctgatgctaa
    19861 catggtgcac gctggtatct acagaggatc gagctaaggt agctgcacca cactccctag
    19921 atgagatcaa ggtgaggtgc tatgtgtagc tgtcacctgt ctatggggct cacagacagt
    19981 gggactcatg tgcatctgtc aaagtacctt gtggcacacc ttgaccatat gcaactttga
    20041 tttgtccatc agacctctac aaagttgaac ggaactggtt tgaccattct gtaaaatgac
    20101 tgccacttgg tagtcatcta cagtgggtga catctagttg gtcctcaccc catgccagct
    20161 gttcccctat tcctccgccc aacaagatgc atcttactgt cacaagctct atcactacaa
    20221 tggcctctgt tgccctcaca accagaagct ggtgaaggcc ttatttcaat agactctaag
    20281 gtccactgtg ctggctagca tcctctgaga ggagggagcc tcagttgggg aaatgtatct
    20341 gtaaggtagg gctgcaggca agcctgtagg gcatttttgt ttgtttgttt gtttttttcc
    20401 catttttatt aggtatttag ctcatttaca tttccaatgc tataccaaaa gtctcccata
    20461 tccacccacc cccactcccc tacccaccca ctcccccttt ttggccctgg cgttcccctg
    20521 tactgggaca tataaagttt gcgtgtccaa tgggcctctc tttccagtga tggccgacta
    20581 ggccatcttt tgatacatat gcagctagag tcaagagctc cggggtactg gttagttcat
    20641 aatgttgttc catctatagg gttgcagatt tttaattggt gattgctgtg agagggccca
    20701 gtccattgtg ggtgggacaa tccctaggct ggtggtcctg ggtcctataa gacccagggc
    20761 ttagggctca gtggttaaga acactgactt tttcctgagg tcctgagttc aattcccagc
    20821 aaccacatgg tggctcacaa ccatctgtaa tgggattcga tgccctcttc tggtgtgtct
    20881 gaagacagct acagtgtact cctgtaaaat aaataggaag aaaggaagga taggagggag
    20941 ggagggagga agggagggag tgggtaggac cccaagactg aggagatggg accccaactt
    21001 catgtgccat gcccagcctg gcttgatggt gagttcttct tcttaagagc taatgaagga
    21061 gaggcattta tcatgtctag acagagagag aaaagacatg tgcccacttt acccagttac
    21121 caagtgacag agcaggcatt gaactgggct ccagagaaca cccaacttct gtgacaacag
    21181 aggtcctcag actgccagca ttgatgtcac ccaagaaatt gctagaaatg caaaat
//