LOCUS       27268#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20731 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 27268
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000102710
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7923..8025
                     /db_xref="ENSEMBL:ENSMUSE00000662362"
                     /rank="2"
                     /note="ENSMUSE00000662362"
     misc_feature    4275..8861
                     /note="5 arm"
     misc_feature    8889..8913
                     /note="B1 Gateway"
     misc_feature    8926..8973
                     /note="Frt"
     misc_feature    8974..9473
                     /note="mouse En2 intron"
     primer          complement(9042..9063)
                     /note="LRPCR LAR3_EN2"
     primer          complement(8938..8961)
                     /note="FRT3"
     primer          13139..13157
                     /note="seq primer NF"
     misc_feature    13199..13206
                     /note="RE sites"
     misc_feature    14999..15032
                     /note="loxP"
     misc_feature    complement(13207..13238)
                     /note="Rox"
     misc_feature    complement(14959..14990)
                     /note="Rox"
     misc_feature    13239..13260
                     /note="polylinker"
     CDS             13819..14415
                     /note="puromycin N-acetyl transferase"
     misc_feature    13934..13939
                     /note="Mutated RsrII site"
     intron          9474..9848
                     /note="mouse En2 intron"
     misc_feature    9846..9848
                     /note="Splice Acceptor"
     exon            9849..9902
                     /note="mouse En2 exon"
     primer          9749..9770
                     /note="seq primer FCHK2"
     misc_feature    9903..10025
                     /note="1Dleader"
     misc_feature    10026..10077
                     /note="F2A"
     misc_feature    10173..10889
                     /note="EGFP"
     misc_feature    10083..10172
                     /note="3X NLS"
     misc_feature    12955..13193
                     /note="SV40 polyadenylation site"
     misc_feature    11988..12932
                     /note="ERT2"
     promoter        13271..13782
                     /note="PGK promoter"
     misc_feature    14526..14958
                     /note="PGK pA"
     primer          13013..13036
                     /note="LRPCR RAF5"
     misc_feature    10899..10952
                     /note="T2A"
     misc_feature    10949..10949
                     /note="A to G to kill RsRII site"
     misc_feature    10953..11978
                     /note="Cre"
     misc_feature    9864..9903
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    9864..9879
                     /note="FCHK QC prefix region"
     misc_feature    9880..9897
                     /note="FCHK QC critical region"
     misc_feature    9898..9903
                     /note="FCHK QC suffix region"
     misc_feature    15055..15075
                     /note="B2 Gateway"
     misc_feature    8862..15105
                     /note="Synthetic Cassette"
     exon            15521..15542
                     /db_xref="ENSEMBL:ENSMUSE00000662361"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000662361"
     misc_feature    15106..15716
                     /note="Critical Region"
     misc_feature    15740..15773
                     /note="Downstream LoxP"
     misc_feature    15717..15796
                     /note="synthetic loxP region"
     exon            19413..19651
                     /db_xref="ENSEMBL:ENSMUSE00000165654"
                     /rank="5"
                     /note="ENSMUSE00000165654"
     exon            18288..18339
                     /db_xref="ENSEMBL:ENSMUSE00000165678"
                     /rank="4"
                     /note="ENSMUSE00000165678"
     misc_feature    15797..20731
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatacgta gcatggagtt catctgaatg aagcatataa cacagaagat
     4321 tttagtgcat ctatagatta tgcaaccttc cctgtagttc agaatttgaa tattttcctc
     4381 atccttagta gaacctgtct tcattaccag ctactctttc ttgaactccc ttctacagct
     4441 gctccaggct tagaccatca cttactgtct ctattggttg tccctactgg acatttcata
     4501 cacacaaatg gaaacaggta atatgtgggg gtagaggttg ccactgtctt cattcactga
     4561 gtatgatttg taggtttatc cttggcagtt tgaatcagat gttcaattac acattccata
     4621 actagtagaa catttaggca tagatactta tgagagtgag ttcaacacac ttcatctaca
     4681 cactgaacac tataccttat tgctttagga tttccattgc tgtgaagaca caccatgacc
     4741 aaggcaactc ttatataaag gttaccattt cattggggct ggcttacaga ggtttagtcc
     4801 attatcatca tggtgggaag catggcagtg accagacaga cttgatgctg gaggagccat
     4861 ctatgggggg ctattctatt caaatgacca cacacagttt taatttttgt ctctttgaaa
     4921 taagtaagtt aaactccata ttgagactta atggcctata ctgaggtatt ttgttagttc
     4981 ttatgtttat gcttggttct ttttcataat cctttctagt tactctttac ttctgaagtc
     5041 cactctgtat tagttcactc tgcatcctcc cttcattctc aatgtcagga tactgacaaa
     5101 ctgacttttt tgacccaggt taaaagttat ttttcctcag cactttgaag atgtttatct
     5161 gctgttttcc agtattagtt actgataaaa aatatatctg tcagttatta tcacttattt
     5221 catagattta agacaggttg tagttattct tgatgctcta cactttccat ataaattgtc
     5281 tattgactga tttttgttta tgctgatcaa cccttggaat ggctaacagc tgttgttgct
     5341 gtaacaaaat accttccaca aacaagagta aaggttaatt ttgtctcatg gctaactcta
     5401 cttcttgagg ccctaggcaa ggctgaataa catggtggtg ggtgtgagtg aaggatgaga
     5461 cttctcaccc tgtcatgact aagaaacaga gagagagaca tgaaggagcc aggacatgtt
     5521 ctcagtgact caccttctac agttccacca tcttccaatc gaatattcca attctgggtc
     5581 aggaaatgga ttactcaatt tatgaggaca gaatcctcac tatctaatca tttcctcaaa
     5641 gtgtggggag cgggtgtggc ggcagtccca aaggcgccag agactgcagc taagtcgtat
     5701 gacttgcacc tgacttcctc atataagcca caaacatctt gagtgctgcg caggtgtacc
     5761 aggatacagg tgaatccaat ttggtggaga tttgcccctg ctgccctgat tagctgaagc
     5821 tgcgtgcctg gtgaggtggc gtggcctgcg gtgcgtggat gagagagagt ataaaaggag
     5881 tgagaggccc agggttcgga ggagatataa aaacaaggga gatataaaaa caagggagat
     5941 ataaacaggg gagatataaa aacaagggag atataaaaac aagggagata tatggagaaa
     6001 gaagaaacag gactgaataa atgtgtgcag aaggatcctg tagcagcgtc gttcttcctg
     6061 gccagttgag cgcgcgcaac atcaaagccc cataactgtg catcactcct ttgcacaggg
     6121 accaggcctt caaggcatgc atgacataac atagaatcta gtgttaatta tatcactgct
     6181 tttagaaaat tgctagcaat gatctaatta ctgtatctgt catttccatc ctctgtgatc
     6241 ccaattaggt aaagctgaga gatttttact agtccatctt caactctttt atatgcatag
     6301 tatgcagatg taatcatata tatacacaca gcatgtgtat atgtatatgt aacatatacc
     6361 actgtctcct tatataacct ttatagatct gcatttcatc atgagctata agtccttggg
     6421 caacgattgg cactaagagt tcaggagcct tctagcagtg ctgggggtct agaatagtca
     6481 atagctccac ctagaaagga gctctttaat ctgctttttt caggagctaa actctcaagt
     6541 ctcccatatg tatctagaac ctatagtgaa aataccaact ggggtgctct tttctcttcc
     6601 acttaagctt cattttattt tgattgttta tttttattgt cttgagcctg cttgaatttt
     6661 taaaaacacc actcatggtt gatatgtatt caaagtagag aaaactcata aagagagctg
     6721 tgattaaaac taatgctaac ttcttgtttt attcagggcc ttctctgctt catctagact
     6781 caccttcccc tggggctcta cttacgaaag gccactgaca ctacctgaaa ggtcttgggt
     6841 gttattattg taccatgtat actaaagaat agcatcaact gttttgttct tttagtacta
     6901 taatgatgat ttgttaaaaa taggtaaaac acatataaac agaaagagca cataaattta
     6961 taaacaatca ttgtctactc accaactaaa gaaaatcatc ttctttcttt taactagatg
     7021 gtcttttatt gaaatatttc ctcttgttgc ttcttagcta gctggacact ccactgcacc
     7081 actgttgatg tcatctatga catcatgagg atggcagcca ttcatagttc aacccacaga
     7141 ctatgtagta cccaggttct taatatttcc agaaagctct ctggctaaag accactgtca
     7201 catatgctag gcaatgttga taatctcatt aaaagtaaca tttctattgt gcttaatgtt
     7261 ctcttgtttt tttttctgtc tcttggtggc tctgagaggg ttttggttgt catgaagagg
     7321 cagagggcac cacctaaatc tggttctgtc tgttctagag tgtcagtgtc atgctaatga
     7381 catgtcagtg tccagttact gattgctttg gtaataccat gaccaacatt tttttttttt
     7441 gaggcagacc aaaggaacca atcttgatga ccaaggcaga tgtagcagtg gtccctgcca
     7501 atggtgaacc tcacatgcac aattttcata tccttaggga caaagttggg tggcacggtg
     7561 taggctgttg gtatctgatg aacctgaatt tggaagactg aagaaagctg tactgtagct
     7621 tcctctgagc caaaagctga aaagcaagtc aaccatttca actgggctac cttttgaaaa
     7681 gttggcaaaa gtcatttgca taacaattaa tgaacacatg actctaatcc attctcagtt
     7741 tctttcctaa tatttagtct ctttcattgt tatgttatta aattataaga tctggggggt
     7801 tttgtttgtt tgtttgaggc aaattataga agcttgtttc ttgagccaag tcccttgctt
     7861 atttagctac ttttgttaaa gagaaaatgt ataataattg cagcaactgt tttattttat
     7921 agggttgttg agtgaagatt gcagacacca tgtctgactc agtgattctt cgcagtgtga
     7981 agaaatttgg agaggagaat catgctttcg aatcagatgg tttccgtaag cgagtttcct
     8041 tgatccttgt gtaaagaaaa ctaaagacaa gagtgagaca cgcctagtga ggtttcaaaa
     8101 agcagagaaa aatcagctac aacatggggg gctagggcta ggcatgtgcc tgacatacaa
     8161 ccaaactgga cgggctctct agaattcatg tcacatctga aagctagctc tgtgttaaaa
     8221 tgcacaacat gagtgagtgc taacatctcc catgagcact tccttctcag tagagtgatc
     8281 actgtataac tttagcccca tagcattgcc gatcaccatg gcaacgataa ttgttaatgg
     8341 tttctgctcc ttaaacaggc cctaccaaca ctctcctggc agaaatgagg ggccttgaca
     8401 cattagtcct tggcatgttc taagctgcta ttcatccatt cattcattca ttcacgaatc
     8461 aaaacaatcc atgcaatcat gcttctaatc cagttttcca attttcaagc cctttatcca
     8521 cactataatg tctctttagt ctttttctgc aactgtatta aatgtcattg gaaccaggaa
     8581 atggtggcca tggtaacaaa tattccttag agttcttttc tctgtggctt ccttatgtcc
     8641 ctgtagacac tcacttccat ttcctactga catctgctct acatgggctg acaaaaataa
     8701 tatgtagctc tgtcttatgg attctggtgg cgatgaggta ttaaagacat gttacagtca
     8761 atttgtgatc tgttaagtag ataaattttg acaaatagaa aatgtttcta aggaaccaaa
     8821 caatagatta cttttcatct taatagataa taccaaatga caaggcgcat aacgatacca
     8881 cgatatcaac aagtttgtac aaaaaagcag gctggcgccg gaaccgaagt tcctattccg
     8941 aagttcctat tctctagaaa gtataggaac ttcgaaccct ttcccacacc accctccaca
     9001 cttgccccaa acactgccaa ctatgtagga ggaaggggtt gggactaaca gaagaacccg
     9061 ttgtggggaa gctgttggga gggtcacttt atgttcttgc ccaaggtcag ttgggtggcc
     9121 tgcttctgat gaggtggtcc caaggtctgg ggtagaaggt gagagggaca ggccaccaag
     9181 gtcagccccc cccccctatc ccataggagc caggtccctc tcctggacag gaagactgaa
     9241 ggggagatgc cagagactca gtgaagcctg gggtacccta ttggagtcct tcaaggaaac
     9301 aaacttggcc tcaccaggcc tcagccttgg ctcctcctgg gaactctact gcccttggga
     9361 tccccttgta gttgtgggtt acataggaag ggggacggga ttccccttga ctggctagcc
     9421 tactcttttc ttcagtcttc tccatctcct ctcacctgtc tctcgaccct ttccctagga
     9481 tagacttgga aaaagataag gggagaaaac aaatgcaaac gaggccagaa agattttggc
     9541 tgggcattcc ttccgctagc ttttattggg atcccctagt ttgtgatagg ccttttagct
     9601 acatctgcca atccatctca ttttcacaca cacacacacc actttccttc tggtcagtgg
     9661 gcacatgtcc agcctcaagt ttatatcacc acccccaatg cccaacactt gtatggcctt
     9721 gggcgggtca tccccccccc cacccccagt atctgcaacc tcaagctagc ttgggtgcgt
     9781 tggttgtgga taagtagcta gactccagca accagtaacc tctgcccttt ctcctccatg
     9841 acaaccaggc tcccaggtcc cgaaaaccaa agaagaagaa cgcagatcgc agatcgcaga
     9901 tctctagagt caccgagttg ctttaccgga tgaagagggc cgaaacatac tgtccaaggc
     9961 ccttgctggc aatccaccca actgaagcca gacacaaaca gaaaattgtg gcaccggtga
    10021 aacagacttt gaattttgac cttctcaagt tggcgggaga cgtcgagtcc aaccccggac
    10081 cggtgcatgt ggaccccaag aaaaagcgga aggtggaccc taaaaagaaa cgcaaagtgg
    10141 atcctaagaa aaaaagaaag gtgcacgtgt ccatggtgtc caagggcgag gaactgttca
    10201 ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg
    10261 tgtccggcga gggcgaaggc gacgccacat acggaaagct gaccctgaag ttcatctgca
    10321 ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc
    10381 agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc
    10441 ccgagggcta cgtgcaggaa agaaccatct tctttaagga cgacggcaac tacaagacca
    10501 gggccgaagt gaagttcgag ggcgacaccc tcgtgaacag aatcgagctg aagggcatcg
    10561 acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca
    10621 acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc
    10681 acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccctatcg
    10741 gcgacggccc tgtgctgctg cctgacaacc actacctgag cacccagagc gccctgagca
    10801 aggaccccaa cgagaagagg gaccacatgg tgctgctgga attcgtgacc gccgctggca
    10861 tcaccctggg catggacgag ctgtacaagc ctgcaggcga gggcagagga agtcttctaa
    10921 catgcggtga cgtggaggag aatcccgggc cgtccaattt actgaccgta caccaaaatt
    10981 tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg atggacatgt
    11041 tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc gtttgccggt
    11101 cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg
    11161 ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtaaaaact atccagcaac
    11221 atttgggcca gctaaacatg cttcatcgtc ggtctgggct gccacgacca agtgacagca
    11281 atgctgtttc actggttatg cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg
    11341 caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca ctcatggaaa
    11401 atagcgaccg ctgccaggat ataaggaatc tggcatttct ggggattgct tataacaccc
    11461 tgttacgtat agccgaaatt gccaggatca gggttaaaga tatctcacgt actgacggtg
    11521 ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca ggtgtagaga
    11581 aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgtc tctggtgtag
    11641 ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat
    11701 ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca actcatcgat
    11761 tgatttacgg cgctaaggat gactctggtc agagatacct ggcctggtct ggacacagtg
    11821 cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg gagatcatgc
    11881 aagctggtgg ctggaccaat gtaaatattg tcatgaacta tatccgtaac ctggatagtg
    11941 aaacaggggc aatggtgcgc ctgctggaag atggcgatct cgagccatct gctggagaca
    12001 tgagagctgc caacctttgg ccaagcccgc tcatgatcaa acgctctaag aagaacagcc
    12061 tggccttgtc cctgacggcc gaccagatgg tcagtgcctt gttggatgct gagcccccca
    12121 tactctattc cgagtatgat cctaccagac ccttcagtga agcttcgatg atgggcttac
    12181 tgaccaacct ggcagacagg gagctggttc acatgatcaa ctgggcgaag agggtgccag
    12241 gctttgtgga tttgaccctc catgatcagg tccaccttct agaatgtgcc tggctagaga
    12301 tcctgatgat tggtctcgtc tggcgctcca tggagcaccc agtgaagcta ctgtttgctc
    12361 ctaacttgct cttggacagg aaccagggaa aatgtgtaga gggcatggtg gagatcttcg
    12421 acatgctgct ggctacatca tctcggttcc gcatgatgaa tctgcaggga gaggagtttg
    12481 tgtgcctcaa atctattatt ttgcttaatt ctggagtgta cacatttctg tccagcaccc
    12541 tgaagtctct ggaagagaag gaccatatcc accgagtcct ggacaagatc acagacactt
    12601 tgatccacct gatggccaag gcaggcctga ccctgcagca gcagcaccag cggctggccc
    12661 agctcctcct catcctctcc cacatcaggc acatgagtaa caaaggcatg gagcatctgt
    12721 acagcatgaa gtgcaagaac gtggtgcccc tctatgacct gctgctggag gcggcggacg
    12781 cccaccgcct acatgcgccc actagccgtg gaggggcatc cgtggaggag acggaccaaa
    12841 gccacttggc cactgcgggc tctacttcat cgcattcctt gcaaaagtat tacatcacgg
    12901 gggaggcaga gggtttccct gccacagctt gatgaagatc tgagctctta attaatgatc
    12961 ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct cccacacctc
    13021 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
    13081 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    13141 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgt
    13201 ttaaactaac tttaaataat tggcattatt taaagttaca tatggcggcc gccctaaggc
    13261 tcgacctcga aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
    13321 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
    13381 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
    13441 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
    13501 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
    13561 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
    13621 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
    13681 gcgggcgccc gaaggtcttc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
    13741 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcatcca tctagatctc
    13801 gagcagctga agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
    13861 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
    13921 cacaccgtcg atccagatcg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
    13981 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
    14041 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
    14101 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
    14161 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
    14221 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
    14281 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
    14341 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
    14401 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
    14461 cgaccccatg catcgatgat atcagatccc cgggatgcag aaattgatga tctattaaac
    14521 aataaagatg tccactaaaa tggaagtttt tcctgtcata ctttgttaag aagggtgaga
    14581 acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga
    14641 ttagataaat gcntgctctt tactgaaggc tctttactat tgctttatga taatgtttca
    14701 tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca
    14761 ctcatgatct atagatctat agatctctcg tgggatcatt gtttttctct tgattcccac
    14821 tttgtggttc taagtactgt ggtttccaaa tgtgtcagtt tcatagcctg aagaacgaga
    14881 tcagcagcct ctgttccaca tacacttcat tctcagtatt gttttgccaa gttctaattc
    14941 catcagaagc tggtcgagta actttaaata attggcatta tttaaagtta ggcgcgccat
    15001 aacttcgtat agcatacatt atacgaagtt atgtcgagat atttaaatat tagacccagc
    15061 tttcttgtac aaagtggttg atatctctat agtcgcagta ggcggtagaa atggtgattt
    15121 ccatcaagcc cagtagctca catctgtaat tctagtccat gggagactga agcaggaaaa
    15181 ttaccacaag tctgaatttg aggctactct tgtctatgta gctcccaaac cagcttacat
    15241 tacagagtga gagaccctgt atcaaaaagg gaaagacttc cttttgatat tcagaaaata
    15301 caaatatttt ataatctgga aaaaatatcc aaacatatag tcaaagcagc aggaaatggg
    15361 gagagaagga gggatgataa agagatagaa agagacagtt attagtttat aatatttcat
    15421 gaataaaagt gcttcctaga aatgcttttg gttgactaat gattatgttg cgtttggaca
    15481 caattattaa cctttttata cgttgtcttc atcctgacag ataacaatga taagaaatca
    15541 aggtgagtga ccgtttcaac accacagcat cttctctaga aggatttcag aatatttcta
    15601 taaatatagg acagagtatt ttcatctacg ttactatgta tagaaagtat agaacacctt
    15661 ttcttttttc tttttccaag acagggtctc tgtgtggctc tggctgtact ggaactgaga
    15721 tggcgcaacg caattaatga taacttcgta tagcatacat tatacgaagt tatggtctga
    15781 gctcgccatc agttcacctt ataggatagc ccttcctgat tatgcccagt ttcctccctt
    15841 gcatcccagt ctaccacaga gcttctagcc tgaacaaaca ggaaagagct ggacaatagt
    15901 gtgattttat gagttccagg aggtcctcca actccactcc tgccccctcc tccaactcta
    15961 caccttctgc tcactcctcc aaattccccc atctccccta tttcccaacc cctggagact
    16021 aataaaaacc taaagttgaa ttctctgagc tttctcttca gcactcgttt gagttcacgt
    16081 gtaaggaagt gttgaggcag gattggatgg ggctgcatta gaacagtggc accgtgtgag
    16141 tacctgacat ggaagatgga tggcagaagg ttgatttaga aagtttaaag tatttcagga
    16201 cagaaggcag cagtagtact ggtaagatta gcactttgct agaagaaatc tggtgaccct
    16261 ggtcctccaa gagtcccatt gcgagtgcag aaaagaaaca tctattataa atccttcact
    16321 gcaagaaggg cattttattt ttttcatttt ttaaatatat aatgtatttt gatcatgttt
    16381 tctgcctccg attcctacca tgttctcccc catttccctc ctacccaccc taccttagtt
    16441 ctctctcaat ctcaaaaaaa aaaccttcaa aaaaaatgaa aataacaaac aaaaacccaa
    16501 caagaccaag aatatcaaaa caaaattaaa ggaatcaaaa agcccacaac agccgggagg
    16561 tggtggcaca ggcctttaat cctagcactt gggaggcaga ggcaggcgga tttttgagtt
    16621 cgaggccagc ctgatctaca aagtgagtgg caggacagcc agggctacac agagaaaccc
    16681 tgtctcaaaa aatcaagaaa caaaaacaac aacaacaaca aaagcccaca acaagcaaac
    16741 aattatggaa ttaattttgt gtcagtcagt cctgggcatg gggcctcttt gctctgtact
    16801 gtgattgata agcccagtga cattctgttg gagaaaatgg attatccctt tgccagcagg
    16861 catcgattgc aagaggttaa agcccagagc ctgtgcccac ttcttcctct cagtgctggg
    16921 accccatctg gtttgaacct gtgcaggttt caggtgtgtt gacacagtct ctgtgaattc
    16981 ccgtgtgcac ccgtcctctt gtactctctg gggtccttca tcctctctgg ttcttacatc
    17041 tttttgcatt ataaatctct gagccccaag gagaggggtt tgatgagtgc tccaaagtct
    17101 cttcctcttt gcaggttttc agttgtgaat ctctgtgtta attcccagct actccaagaa
    17161 gaagcttctc tgagcagggc cataggatgc tctgacccag ggctttagca gtaggccatt
    17221 aggagtcatt acactgctat gtttctttag cagaataata gtggtacatc cctctaggct
    17281 catgaggaaa gtatatttat gggcagatag aataaacatt ataaatttag aaaacataat
    17341 acccggtagc tactgagaat gcataactat tttttttcct aagaaagaga ctgaattgga
    17401 gatgaagctc tgagaaggaa tgtgtgtgtc agggcagatg tcattgaaat cttaacgatc
    17461 ttggctagag aaggtcctga caaggagact taggagactt ggagtgagaa tctgaagttg
    17521 gagtaactgt ccgcatgagg gtctaagaaa ggacattttg agctggagca catttggcaa
    17581 agataaatgg atgggtgatt gttttcatga caactagaat atatacacta ttactataca
    17641 ttataattgt tctatatata ttctatgtat ccatatctct atctgtatgt atatatagaa
    17701 catatattaa tatatataga acatgtaaaa tatatataga atatatggta tagtgtatat
    17761 atatatattt agaataaata tacaaatata tacacacata tatacaaatt atacaatcaa
    17821 taaatataca aaaatatata taaatatttt atattgtatc tcctgctgag tccatttaat
    17881 gttgcctgta cacatggcct aaggctgact gttggattat gggcaatcta ctaagaatct
    17941 tactcctgaa ttaaacagag cccagcccct gctttcagct gttattgatt acctgtaact
    18001 cttcaactat gtgtccgacc ttgtggactc ccctactccc ccccccccac cccctgtcct
    18061 catgctgtaa tgtagattgg tatggcccta tacaagtctt gtccaggcac ctacagctgt
    18121 tgggaattca tcagtgcaaa atacattaaa atactgggtt tggccagaat tcccagccag
    18181 aggggaaatg catacttatt aaagaataat tcagaaaagc accatgtagg gagaatttct
    18241 caccagtttt gggaacatga cctttaaatg cctttccttc tcttcaggtt acaagataag
    18301 aagaaagggg aaggcgctcg agttggcttc tttgaactgg taaataatgc actgtgctgc
    18361 tccccttggc aactttgcaa ctgtgagtta ttcccacagt agaggaagag ccaactcctt
    18421 agtcatactg gcaggactta actctaggga tcagtcttgt agtcatgagc tttaccgaac
    18481 atatgaatac tggttaaaat ccaatgctta gtaaacccac tggctaatat cagtggaata
    18541 gaatccatcg acacacaatt ataatcttaa taaggaaagc ctttacagta actcccaggg
    18601 caatagatca catatataca ttgttatgag aagtctttag aaattccata gcttatatat
    18661 ctaccacaaa tttgtatagg atgcaggcat ttcctctttc tttctttctt tctttctttc
    18721 tttctttctt tctttctttc tttctttctt tctttctttc tttctttcct tccttcctcc
    18781 ctccctccct ccctccctcc ctccctctct ccctccctcc ctccctccct ctctctctct
    18841 ctctctctct ctctctttat ttctttctct ctttctccaa aatagtgttt ttctgtagcc
    18901 ttgcctgccc tggaatttgc tctgtagact aggctggcct ccaactcaga gatctgcctg
    18961 cctctggctc cggagtgctg ggattaaagg ggtgtgccac caccgtttag gatggagcat
    19021 ttttaagcaa caatttgcct gttgtccgga tgggttctca gagctgagag gcaacttcct
    19081 gcgctgttaa atgttcactc agtatgtcta cctggtaatg gcatctgtga tgaagacttg
    19141 gaaggtagta aagtcagctc ctggtctgag gagaagaggc tcaaagattt gggcgtaata
    19201 taaggatctg atgttttaga caattgatag ctagtacttt tacataatta gtgtgaagac
    19261 aactgctcag agatgacccc cacaagcacc tggtgttgaa tgttgtttaa cagaatagag
    19321 gtgaaattaa atagccctcc tgcccatccc cccaccccat ctcccatgtg ctttttacat
    19381 atagataatc tctgaaccct ttgtttttca agtttcgatt ttcttcatca aaagacaact
    19441 ggctgatgtt tatgggaagt gtgtgcgcat tgctccatgg aatggcccag ccaggcatga
    19501 ttattgtgtt tggtatactg acagatattt ttgttgaata tgacattgaa agacaagaac
    19561 tcagtattcc agaaaaagtg tgtatgaata acaccattgt atggatcaac agctccttca
    19621 accagaacat gacaaacgga acaagctgtg ggtaagctac ttttcttttt tgttacacct
    19681 taaattataa ttcaaaacat ccaaagacaa taccgactga gttggaagtg gcatttagta
    19741 cagaagaata gaaaaacaaa cttacccata ttgataaaat tctttaactt tgtagctatt
    19801 tccatccttc caggtttcaa agcatcaaca gaccttgcta atcaaatacc tacatcaaca
    19861 attgactact tggctcacta aaagtccaca catgacaata tccatactta cgaccactaa
    19921 gaagataatt gaatttcact tgagtctcct ggacaaaagc tcctttctgc tcaccacccc
    19981 tgcctttttt tttctttttt ctttttaccc cttggctttg ttttctgtcc atgtgtttca
    20041 tgagcatgct atattagagg aatatgaatc aatagttcta gcgaatagtt agttaagttt
    20101 tcatcaagag ggctgcacct atgacaaaga aggtagccct gaagaactag gcagaacatt
    20161 atggaaatca gacaaggtcg gtcactgtgc cagagttgtc tctgtgacac gcccaacagc
    20221 ccactttgta atcactgagt ctgtagtaca tgtgggctga ctcactgttg tggctctgac
    20281 tttgtacctg cctaatgctg ctttggatac aggaccaagt tcattggtat gtatgagtcc
    20341 ctgtgctcgg gttcatgact caaaggcaga gcatatcctg cctagaaaga cacttggatg
    20401 gctttctcaa atacatcttt atttttcata ttaattactg aaatgtggac agggttggtt
    20461 taaactgggt ctttctaaat cattaaaatt tttccataat atcatctact ttgaggattt
    20521 ttcactgctt caatcaaaaa aatttgaatg tcaactaagt aacatgaatg tgtagaagca
    20581 tcagggaagg acacactgtc ctatgagtca catccttatt ttgtagaact ttggttagtc
    20641 actcagactg tcattcactc aatggtcttt tataaaatat tagttacaaa ctagtgagtg
    20701 ctgggtgatg agaaaacata aaattctatg t
//