LOCUS       230475#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20852 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 230475
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000025257
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            9854..10028
                     /db_xref="ENSEMBL:ENSMUSE00000141961"
                     /rank="2"
                     /note="ENSMUSE00000141961"
     exon            9265..9402
                     /db_xref="ENSEMBL:ENSMUSE00001003881"
                     /rank="1"
                     /note="ENSMUSE00001003881"
     misc_feature    4275..10161
                     /note="5 arm"
     misc_feature    10189..10213
                     /note="B1 Gateway"
     misc_feature    10226..10273
                     /note="Frt"
     misc_feature    10274..10773
                     /note="mouse En2 intron"
     primer          complement(10342..10363)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10238..10261)
                     /note="FRT3"
     primer          14439..14457
                     /note="seq primer NF"
     misc_feature    14499..14506
                     /note="RE sites"
     misc_feature    16299..16332
                     /note="loxP"
     misc_feature    complement(14507..14538)
                     /note="Rox"
     misc_feature    complement(16259..16290)
                     /note="Rox"
     misc_feature    14539..14560
                     /note="polylinker"
     CDS             15119..15715
                     /note="puromycin N-acetyl transferase"
     misc_feature    15234..15239
                     /note="Mutated RsrII site"
     intron          10774..11148
                     /note="mouse En2 intron"
     misc_feature    11146..11148
                     /note="Splice Acceptor"
     exon            11149..11202
                     /note="mouse En2 exon"
     primer          11049..11070
                     /note="seq primer FCHK2"
     misc_feature    11203..11325
                     /note="1Dleader"
     misc_feature    11326..11377
                     /note="F2A"
     misc_feature    11473..12189
                     /note="EGFP"
     misc_feature    11383..11472
                     /note="3X NLS"
     misc_feature    14255..14493
                     /note="SV40 polyadenylation site"
     misc_feature    13288..14232
                     /note="ERT2"
     promoter        14571..15082
                     /note="PGK promoter"
     misc_feature    15826..16258
                     /note="PGK pA"
     primer          14313..14336
                     /note="LRPCR RAF5"
     misc_feature    12199..12252
                     /note="T2A"
     misc_feature    12249..12249
                     /note="A to G to kill RsRII site"
     misc_feature    12253..13278
                     /note="Cre"
     misc_feature    11164..11203
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    11164..11179
                     /note="FCHK QC prefix region"
     misc_feature    11180..11197
                     /note="FCHK QC critical region"
     misc_feature    11198..11203
                     /note="FCHK QC suffix region"
     misc_feature    16355..16375
                     /note="B2 Gateway"
     misc_feature    10162..16405
                     /note="Synthetic Cassette"
     exon            16591..16790
                     /db_xref="ENSEMBL:ENSMUSE00000934105"
                     /rank="7"
                     /note="ENSMUSE00000934105"
     misc_feature    16406..20852
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaagtatt ctgtagccca ggacttaatg gtttgcatgg catcgttctg
     4321 aactagactc cgtgttcact acctgtgtgc acatctgtaa atcgagaact gcaaccatga
     4381 ctgtccagac ttcccgccct agctacttag tgtatataag gctctcctcc tacccttggg
     4441 ttggggtgat ccaggagatc accgggccta aacttaagag gactctgctc ctcttacctt
     4501 tctcccaggt aagaaagact tccaaccatc ttaccatagc tatggattct aagacactgc
     4561 atgaatgctt ttgagatcag tccatgtctt agtcaggtta ctattactgt gatgaaatat
     4621 catgaccaaa agaaacttcg gaaggacttt ttgggcttac aggtcctgag ttacactgtc
     4681 cactgaggga agccaaagca ggacctcaaa caggcaggaa cctggaggca ggagctgata
     4741 aagaggccat ggaggtatgc ttcatactgg cttgctcctc atggctgctc agcctgcttt
     4801 ctacagaacc caggatcacc agcccaggat ggccccaccc acggtgggct gggccctcct
     4861 ccaatcatac tgattgcggg cattttctca gttgatgatc cctctttcag atgattctgc
     4921 ttgtagcaag ttgacaaaac tctccagggc agtccatcta gagtaagtca tggcgtatgt
     4981 gtattcaaga acaattttca ttatctcaga ctttctaggg aagttaatag ttttgttatt
     5041 ttgttttgtt ttgtttcgtt ttttttttgt tgttgttgtt gtttttgaca ggatctcaat
     5101 tatgtagttc ttggctggcc ttggaactat gtgtagacca ggctggccct gaactcaaga
     5161 gattcacctg cttctgccgc ttgtgtacta ggattaaagg tgtgcaccaa cacacccagc
     5221 cttcaaaaat atatatgtgt gtgtgtgtgt gtgcgcacac acacacacac acacacacac
     5281 acatatatat actttttaaa aatttatttt tggcttttcg agacagggtt tctctgtata
     5341 gccctggctg tcctggaact cactttgtag accaggctgg cctcaaattc agaaatccgc
     5401 ttgcctctgc ctcccgagtg ctgggattaa aggcgtgcac caccactgcc cggctatcaa
     5461 attatatttt aattaactta agacacatcc tactctgtaa cccaggatac ttgaacgttg
     5521 agtatgtaga tcaggttagc cttaaatatt cagtaactcc ttgaattaaa aaattagttc
     5581 ttgggctggc gagatggctc agagggtaag agcactgact gctcttccaa aggtcttgag
     5641 ttcaattccc agcaaccaca tgatggctca actacccata atgagatttg atgccctctt
     5701 ctggtgcatc taaagacagc tacagtgtac aataataaat aaatcttttt aaaaaaatta
     5761 gttcttaggg ttaaataaat agctcagcca aatagtgttc tggctgctct tccaatggac
     5821 ctgggagtct gtggcagctc gcattctctc ataactcctg cttcagagta tccaacaccc
     5881 tcaattggcc tctggaatag aactggtaca cagacaaaca tgtaggcaaa agacccccgt
     5941 gcccacaaca cacatttttt ttaaagttat tattcaaacc aagcaatggt attttgtcct
     6001 gtatgtcttt attaaaagtg tcactgactt caaatcacag cagttggaag gctgaagtca
     6061 gttgttcatc ttcagctatg tactgagtcc cagggcagcc tgggctatat gagattctgt
     6121 ttcaacaaga tgaaaacaag agatcatggg ctggagagat ggctcagtgg ttaagagcac
     6181 tgactgttct tctaaaggtc ctgagttcaa atcccagaaa ccacatggtg gctcacaacc
     6241 atctataatg agatctgaca ccctcttctg gtgtgtgcct gaggacagct acagtgtact
     6301 tatatataat aataaataaa tctttttaaa aaagaaaaag aaaagagaaa acaagtagcc
     6361 tgtgggctac tgacacttga aagaaatctt ggcagtacag gtacctcaag aggactgctg
     6421 gcctcaggaa aggctgggcc actgtgacct cacagaaagg tagccgtggt gataggcacc
     6481 cagttcctcc atcctattgt ttccctgtca ggctcctcca aggcccaaac taactggagc
     6541 cagacgaacc ctctgatgtg gtctgcacag gtcagtctcc tgggcaaaga gcagggctgg
     6601 aagaatggag ggtaaataga aggctctcag ggcacacaca cgtgtgtaca cacacacact
     6661 ctcgctctct ctccctcact ttctggacag tgcatccttt tccaggcagc acagtcagtt
     6721 cacaggcctt agtcctctag tcacccccgt gaagagcact gaaccacggc agaggctcga
     6781 agcaggccgc ctctaatctc agtgttctga cagcaccttc cctgactcca tccccaggcc
     6841 tcttctgcta cattttgttt gggggttttg gtttttctct taaatttatt tttattctct
     6901 gtctgtctgt gtgcaagggc atgactggac tggttagagg tcggcttgtg gaaatctgtt
     6961 ctttcctgcc tgtctaccac gggggttctc ggttcccact caggtcttca tggtggcagc
     7021 acctcactca cagagcccca ccctaacccc agccctggct tttcttttca gacctgtctc
     7081 aggctcacct agaactccac aagactgctt gcctcaaacc tttggcagtc ctgactcagc
     7141 ctccacagtg ctgggaaaca gtcttgcatc tccctggctg actttttaaa attccatttt
     7201 ttgttttttg ttttttttgt ttttttcgag acagggtttc tctgtatagc cctggctgtc
     7261 tggaactcac tttgtagacc aggctggcct cgaactcaga aatctgcctg cctctgcctc
     7321 ccgagtgctg ggattaaagg tgcccccccc cccccgggct tttggctttt gggcgactgt
     7381 tgctcttaca cacacacaca cacacacaca cacacacaca cacacacacc ttcacactaa
     7441 gatatacaat ctctagcccg gcagtggtgg cgcacgcctt taatcccagc acttaggagg
     7501 cagaggcagg tagatttctg agttcaaagc cagcctggtc tacatacaga gtgagttcca
     7561 ggacagccag ggctacagaa gaaaccctgt ctcaaaaaac caaaaaaaaa aaaaaaaaaa
     7621 aaaaaaaaag atatacaacc tcactcactt cctgagcaaa gctcgtcaca ctcttgcagt
     7681 ttgctaactg ataaaggttt aatacagaat cttctattat agcaaaactc tttttgagac
     7741 agggtttctc tgtatagccc tggctgagca agactctttt aaccgggttt atgttaatag
     7801 ggtgactttt ggaaagtccc taaagatagg aatcttgttg ccaagggcag gaatgaggaa
     7861 gagagctata actttcaact caactggtca gtagccaata gctgattatt taaatagcta
     7921 atgatttaac ggactatccc agatcccagc ctggtggtac ttccttaaac atctgagaag
     7981 atgcgtgtca gagggctgcc agggactgtt agtacaggag ggcacggagg ctccagaccc
     8041 cctctccttg gtacctcgtc tcactgttgc atctggctgc cactgtgttg gatgcgttat
     8101 aatgaagcac agatgtgggc aaggtgcttg gcttgagtcc tgtgaaccag cctggcaaat
     8161 taccagctct gaagaggggc ttgtagcatt cttgacttat agggctggct gtccagaagt
     8221 acaagaggtc tggacttgag accagatctt aacctagagc catcttgtaa ggacagagcc
     8281 cctccacaca aaggattggc catcttcagt gtcagagctg aattctacta taggatgcat
     8341 cgtgaaaacc tcgtttccac caggaactga ggttgctgct ggaggaaatc tcccatgaag
     8401 agctgttaac tggggaactg gttggatcca gctcctacaa gctctccttt aatttccata
     8461 cacatgccaa gctgtgaaca cctgcacaca cagtacaaat aaaacagtag aaatgaaatg
     8521 aaaattaaaa caaaaacagc atactttctc ctttctaccc acatatctcc tgcatccctg
     8581 agagcaattt cttggccatc ttccataatc atgctattca aacctgccct ctaagtacag
     8641 agctctgaat ggagaccacg ggagcagact tttcatctgt tggctccctg ggcctagcag
     8701 tgtaccctgc aaacaggaaa ccttcgctca tcatttatca catgaatgtg gctagagaac
     8761 tgtccccacc agacactgag agcctctcac agggccagag ggtggctgcc tcaaaggcag
     8821 gcgagaggct gcagtcttct gcagagtgga cagatactgc ctgccataca aggggacata
     8881 gcctggggcg gggggcacaa gggaaggggc ctgagtgcat tggagtcaca gccctccctc
     8941 ccctggtgac ccagtgactg aggggaccaa ggctatccct ggtatgaggg gagggcattt
     9001 gcatgagtta gacccctgac agcccatggt ggggacagga agtagctctg tctactgtct
     9061 ctttcagtct cactttctgt tcctccaaag tgtctcttca aactctcaaa tgactttgta
     9121 gattcagcct aagatctcaa agcaggtgag gcaggaggct ggcactcaag agcctgacga
     9181 acacaaaccc aagtccttct gggacatgtc tccatgtgtc cccagtgctc ctctgtagcc
     9241 cctgagagag gtttctttcc tttccagggc gctaggctca gctcacccca ttcctggagc
     9301 agcctgcaga cttcatcctc tctcttccat cccggggaaa gtcagccagt cctcctcagc
     9361 tgcctgtctt aacctgcatc atgaagcctg aggagatttc aagtaaaccc tcccaagccc
     9421 cacctcagga tctggggaaa gccactgtct accgcatcct tggtttgaga caggttctta
     9481 ctgtgttggc ctggctgggt tccaactccc aggatcctct agcctcagcc tctcttaggt
     9541 gctagattgc aggcctaggg tatcacgctc ggtccctcag atcctggtgc ctttccacac
     9601 ctccgggagc tgatctaagt ctttctccca cgtacagtct gcttctttgg aggagctgtg
     9661 tggagctagg tgtgttacag ggctgtagct ccagccatct gggaggctga ggcaggatgg
     9721 ctacttcaat tctcaagagc ctggatagga gaccctgccc ccaccccacc cccaaagtaa
     9781 tttttcagca cattacttct tcatctcctc tctcaacccg ttctgccttc tcctggggtg
     9841 ctggtgtcag cagaagctga tgtggaagtg atgcctggga gttagcaagg gaatgagtgg
     9901 aaaggggaag tgtgagaacg gtcccagaag agactgggga gctggtggag agaggaccca
     9961 gcggacagac tgccagccta agacaaccag cgtctgagga gccatgagcc aaagcaggga
    10021 tttgcagggt agggaaggtg gaccccatag tgtcatgagc cagcgtgagt caggcctggc
    10081 ttggggagcc aggggggcag ctggaggatc tcagagggca gcacttgggt aacacctggg
    10141 ctgggcacgg ggaagaggag aaaggcgcat aacgatacca cgatatcaac aagtttgtac
    10201 aaaaaagcag gctggcgccg gaaccgaagt tcctattccg aagttcctat tctctagaaa
    10261 gtataggaac ttcgaaccct ttcccacacc accctccaca cttgccccaa acactgccaa
    10321 ctatgtagga ggaaggggtt gggactaaca gaagaacccg ttgtggggaa gctgttggga
    10381 gggtcacttt atgttcttgc ccaaggtcag ttgggtggcc tgcttctgat gaggtggtcc
    10441 caaggtctgg ggtagaaggt gagagggaca ggccaccaag gtcagccccc cccccctatc
    10501 ccataggagc caggtccctc tcctggacag gaagactgaa ggggagatgc cagagactca
    10561 gtgaagcctg gggtacccta ttggagtcct tcaaggaaac aaacttggcc tcaccaggcc
    10621 tcagccttgg ctcctcctgg gaactctact gcccttggga tccccttgta gttgtgggtt
    10681 acataggaag ggggacggga ttccccttga ctggctagcc tactcttttc ttcagtcttc
    10741 tccatctcct ctcacctgtc tctcgaccct ttccctagga tagacttgga aaaagataag
    10801 gggagaaaac aaatgcaaac gaggccagaa agattttggc tgggcattcc ttccgctagc
    10861 ttttattggg atcccctagt ttgtgatagg ccttttagct acatctgcca atccatctca
    10921 ttttcacaca cacacacacc actttccttc tggtcagtgg gcacatgtcc agcctcaagt
    10981 ttatatcacc acccccaatg cccaacactt gtatggcctt gggcgggtca tccccccccc
    11041 cacccccagt atctgcaacc tcaagctagc ttgggtgcgt tggttgtgga taagtagcta
    11101 gactccagca accagtaacc tctgcccttt ctcctccatg acaaccaggc tcccaggtcc
    11161 cgaaaaccaa agaagaagaa cgcagatcgc agatcgcaga tctctagagt caccgagttg
    11221 ctttaccgga tgaagagggc cgaaacatac tgtccaaggc ccttgctggc aatccaccca
    11281 actgaagcca gacacaaaca gaaaattgtg gcaccggtga aacagacttt gaattttgac
    11341 cttctcaagt tggcgggaga cgtcgagtcc aaccccggac cggtgcatgt ggaccccaag
    11401 aaaaagcgga aggtggaccc taaaaagaaa cgcaaagtgg atcctaagaa aaaaagaaag
    11461 gtgcacgtgt ccatggtgtc caagggcgag gaactgttca ccggcgtggt gcccatcctg
    11521 gtggaactgg atggcgacgt gaacggccac aagttcagcg tgtccggcga gggcgaaggc
    11581 gacgccacat acggaaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
    11641 ccttggccta ccctcgtgac cacactgacc tacggcgtgc agtgcttcag cagatacccc
    11701 gaccatatga agcagcacga cttcttcaag agcgccatgc ccgagggcta cgtgcaggaa
    11761 agaaccatct tctttaagga cgacggcaac tacaagacca gggccgaagt gaagttcgag
    11821 ggcgacaccc tcgtgaacag aatcgagctg aagggcatcg acttcaaaga ggacggcaac
    11881 atcctgggcc acaagctgga gtacaactac aacagccaca acgtgtacat catggccgac
    11941 aagcagaaaa acggcatcaa agtgaacttc aagatccggc acaacatcga ggacggctcc
    12001 gtgcagctgg ccgaccacta ccagcagaac acccctatcg gcgacggccc tgtgctgctg
    12061 cctgacaacc actacctgag cacccagagc gccctgagca aggaccccaa cgagaagagg
    12121 gaccacatgg tgctgctgga attcgtgacc gccgctggca tcaccctggg catggacgag
    12181 ctgtacaagc ctgcaggcga gggcagagga agtcttctaa catgcggtga cgtggaggag
    12241 aatcccgggc cgtccaattt actgaccgta caccaaaatt tgcctgcatt accggtcgat
    12301 gcaacgagtg atgaggttcg caagaacctg atggacatgt tcagggatcg ccaggcgttt
    12361 tctgagcata cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag
    12421 ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta tcttctatat
    12481 cttcaggcgc gcggtctggc agtaaaaact atccagcaac atttgggcca gctaaacatg
    12541 cttcatcgtc ggtctgggct gccacgacca agtgacagca atgctgtttc actggttatg
    12601 cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc
    12661 gaacgcactg atttcgacca ggttcgttca ctcatggaaa atagcgaccg ctgccaggat
    12721 ataaggaatc tggcatttct ggggattgct tataacaccc tgttacgtat agccgaaatt
    12781 gccaggatca gggttaaaga tatctcacgt actgacggtg ggagaatgtt aatccatatt
    12841 ggcagaacga aaacgctggt tagcaccgca ggtgtagaga aggcacttag cctgggggta
    12901 actaaactgg tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc gaataactac
    12961 ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag ccagctatca
    13021 actcgcgccc tggaagggat ttttgaagca actcatcgat tgatttacgg cgctaaggat
    13081 gactctggtc agagatacct ggcctggtct ggacacagtg cccgtgtcgg agccgcgcga
    13141 gatatggccc gcgctggagt ttcaataccg gagatcatgc aagctggtgg ctggaccaat
    13201 gtaaatattg tcatgaacta tatccgtaac ctggatagtg aaacaggggc aatggtgcgc
    13261 ctgctggaag atggcgatct cgagccatct gctggagaca tgagagctgc caacctttgg
    13321 ccaagcccgc tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc
    13381 gaccagatgg tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat
    13441 cctaccagac ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg
    13501 gagctggttc acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc
    13561 catgatcagg tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc
    13621 tggcgctcca tggagcaccc agtgaagcta ctgtttgctc ctaacttgct cttggacagg
    13681 aaccagggaa aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca
    13741 tctcggttcc gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt
    13801 ttgcttaatt ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag
    13861 gaccatatcc accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag
    13921 gcaggcctga ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc
    13981 cacatcaggc acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac
    14041 gtggtgcccc tctatgacct gctgctggag gcggcggacg cccaccgcct acatgcgccc
    14101 actagccgtg gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc
    14161 tctacttcat cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct
    14221 gccacagctt gatgaagatc tgagctctta attaatgatc ataatcagcc atatcacatc
    14281 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    14341 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    14401 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    14461 gtccaaactc atcaatgtat cttatcatgt ctggatccgt ttaaactaac tttaaataat
    14521 tggcattatt taaagttaca tatggcggcc gccctaaggc tcgacctcga aattctaccg
    14581 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
    14641 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
    14701 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
    14761 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
    14821 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
    14881 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
    14941 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcttc
    15001 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
    15061 ctcatctccg ggcctttcga cctgcatcca tctagatctc gagcagctga agcttaccat
    15121 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
    15181 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccagatcg
    15241 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
    15301 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
    15361 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
    15421 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
    15481 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
    15541 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
    15601 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
    15661 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
    15721 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgatgat
    15781 atcagatccc cgggatgcag aaattgatga tctattaaac aataaagatg tccactaaaa
    15841 tggaagtttt tcctgtcata ctttgttaag aagggtgaga acagagtacc tacattttga
    15901 atggaaggat tggagctacg ggggtggggg tggggtggga ttagataaat gcntgctctt
    15961 tactgaaggc tctttactat tgctttatga taatgtttca tagttggata tcataattta
    16021 aacaagcaaa accaaattaa gggccagctc attcctccca ctcatgatct atagatctat
    16081 agatctctcg tgggatcatt gtttttctct tgattcccac tttgtggttc taagtactgt
    16141 ggtttccaaa tgtgtcagtt tcatagcctg aagaacgaga tcagcagcct ctgttccaca
    16201 tacacttcat tctcagtatt gttttgccaa gttctaattc catcagaagc tggtcgagta
    16261 actttaaata attggcatta tttaaagtta ggcgcgccat aacttcgtat agcatacatt
    16321 atacgaagtt atgtcgagat atttaaatat tagacccagc tttcttgtac aaagtggttg
    16381 atatctctat agtcgcagta ggcggtcttc tccatagccc cagccttgtc ccatcagcca
    16441 ccctttctcc tcagaggagc tcttccgaga cccttcacac acccatccac agctctggcc
    16501 cttcctcgtc cccacttctg cactcttaga gcaacctgag atttactact cccttgagag
    16561 acttgtccct gatgcctgtg tctcttccag gattctgatg tatgaggaga aaaacaaaga
    16621 acacaagagg ccaactggtc ccccagccaa gaaagctatc tccgagctgc cctgattgga
    16681 ggtggatgtc acacggtggg gctgagtgag gagcttctga tgacagcagc atggaaaaaa
    16741 gaaacagtcg tgagccagag tcagactaaa taaatgacgc tcctagtggg tcacatcagc
    16801 tctgcagtta ctggggacac gtgtcctgga cctgggggag aaggaagcat ctttgaggga
    16861 agggtagtca ggattttcca gccttactat tggatggggt tgctatggct gatagctctt
    16921 tgttctgagg ggagattcca gagtttcaag acagcattga cctcaaagat tataggtgtg
    16981 tgcctggtgg tagtggtgca ccccttaatc ccagcacttg gagggaggga tagaactctg
    17041 caagtctgag ttcatggcca gcctggtcta caaagtgagg acaaccaggg ctataaagag
    17101 aaactcttgt cttgaaaaac caaaccaaac caagagagaa tataggtgtg atatctttta
    17161 tctgtccagg ctcacaggag cttcatagcg gtgaagggtg accttgacag agtcttcatc
    17221 tccctgcctg taactctgac cagcgcacag tctctctagc tttactttgg aaataggaat
    17281 gacagctaag ctgtttgttt gtttgtttgt ttgttttgag acgtttctct gtgtaacccc
    17341 ggctgccctg gaactcatac tgtagaccat gttggcctca aattcagcca tccacctgtg
    17401 tcccgagtac tgagactaaa ggtgtgcgcc accactgccc agctaggagc gaagcttttg
    17461 tacttggcct atcagaagtt ctcagcaatg tttgaaacct gagtgaacag atggggtgaa
    17521 ggcctcatga tactataaag ttaaacaaga cgttgggctt ctcatgcaga gaaaccatca
    17581 agtccagtct ggagccagag ctaacgctca gtggtagaat gcttgcctag ccctaggttc
    17641 gattcccagg actgccaaaa gccccccacg ccccctgtcc cgccccggct actaatgaag
    17701 ggaagagggg gtatatcagg aagctaccag aggcaaagat ggtgaattca gcatctctaa
    17761 atgatagaaa ctgtggaaca gcccacaaac caggaaaagc aagggaccta aggacccaag
    17821 gccaggcctt tctctctccg agggatccag gtgagttgtc tctggccaca gaattcagat
    17881 ccactcactg tcctttcctt cctccactgc tcagccgaac ccatggcttc ccatgtactg
    17941 agcaggcact cacggaaaat acagcccccc ccatccagct ttcttttttc cttttttttg
    18001 tttttgtttg tttgtttgtt tgtttgtttt cgagacaggg tttctctgta tagccctggc
    18061 tgttctggaa ctcactttgt agaccaggct ggcctcgaac tcagaaatcg gcctgcctct
    18121 gcctcttgag tgctgggatt aaaggcgtgc gccaccacca cccagtttgg ctgcagattt
    18181 ttttttttat tctttttttt tttttaaaga tttatttatt tattatatgt aagtacactg
    18241 tagctgtctt cagacactcc agaagagggc gtcagatctt gttacagatg gttgtgagcc
    18301 accatgtggt tgctgggatt tgaactccag accttcggaa gagcagtcgg gtgctcttac
    18361 ccactgagcc atctcaccag ccccttggct gcagattttt aaaacctatt tttgattgtg
    18421 tgtacgtatg tgagcatgtt gcacatgagc acaggtgccc taggacccca gaggcacaga
    18481 attcccctgg agctggagtt acaggagcca cctgatatga acgttggcaa ctaaactctg
    18541 gtcctctgca aaatcagtca tacccactat taatcaagag gttgccttca atgttccatc
    18601 cctccctcct ctcttctctt tcctttcttc cccctcctct ctccttttca tctctctctt
    18661 ctctctccct ctccaaggtt cacttgttac atagcataaa ctggcctcaa actcacaatc
    18721 ctgctttagt cttttgtgtt ctaaccagca accaagctct ttaagtaaag gtcttggttg
    18781 gtgctgcagc actagcgccc tctagtgtaa aactctcatg tagctttttt tttttttttt
    18841 ttaatttcat gtatataagt acaccgtagc tgtcttcaga cacaccagaa aagggcatcg
    18901 gatcccatta cagatggttg tgagccacca tgtgattgct gggaattgaa ctcaggacct
    18961 ctggaagagc agtcagtgct tttaactgcc gagccatctc tctagcccct catgtagctc
    19021 ttgtgtaggt agggagagga atcaggatgc ttcagtcctt ggcagcaact ggaatggcag
    19081 cttcctggca ggtgagccta gcatccttca gcccttgaca cctggtagca ttacagatgt
    19141 tggccagggg tatccagaac ctgtccagaa aacttgacag ctctctatat ctcctaacaa
    19201 tgactgaggc tgcagtttct tctctctgga tctttatttt taattattac tttacagata
    19261 tgaatgtttt gtcttcctgt aagtctatgg accgcatgtg atgtgtgtct ggtgcctgtg
    19321 taggccaaaa gaggccatca gatccctggg actagaatta cacactgtta tgagccatca
    19381 tgtgagtgct ggaaattgaa ccctggtcag ctatgtgtgt ggcgagtgtc ttaacttctg
    19441 acctatccca ccagttatcc ccccaccccc ccaggcattg tctcactatg tagaccaggc
    19501 ttgacaggaa ctcacaaaga tctgcctgcc tctgcttcct gactgttggg actcatgggt
    19561 gcactaccag tcctctctgg tttattaaag tttcctactc ttcagcctcg gttctccacc
    19621 ttttctaccc ctgtttttca ccttgttcct gtctgcatgc tctcaaaccg atgggctgcc
    19681 gcctcttcag ctgtcaggca gtctgcctcc gccatgctct gccactttct ccactgccag
    19741 ctcagcccac actactaaaa gcaggcaaga gaggatcatc aaaaaggagt gtttaattgg
    19801 gtccaaggga gccagcatag gagtgctgtc cgggcaactt ccaagtgagc cagacatctt
    19861 tgcttatata ccccacaagg ggtagcgcca gtcacagcaa atctctcctg caaatgctct
    19921 gaattgtaaa actcatacaa ttgtcccttc agcaccagtg acagcagcat cgctactagc
    19981 ccaccatgtg ggaatggcag aaggtcacac agagtggtga aagtcacaca tggatgtctg
    20041 tgatagcaaa aaggatgagt acaaactgag tagccctggc tgaggacaat gagctaaaat
    20101 tgtttctcca atattgtaga gtgttctaaa gagtgtatat tgagctggtc ggcagtgatg
    20161 cacaccttta atcctagatc ccagcacttg ggaggcagag gcaggtggat ctctgagttc
    20221 aaggccagcc tggtctacag agtgagttcc aggacagcca gggctacaca gagaaaccct
    20281 gtctcaaaac caaaacacta aaaaaagggg cgagggttat attggtaaag agtatagtct
    20341 ggctagcttt tgactgactg cccttcctct taagccagtg taggtaggta gggagagtcc
    20401 aacaacaagg cactaatgac tgagtcaaac tgtcgcatgt tccagtgtct tcggctcaca
    20461 ggaaagtgat cagaagagta cttaccttaa agaacttatt acttaataac aatttggaga
    20521 gattttaaca gtgctgagta cccagtgccc caagtggggt gtttcaatat ttataagcac
    20581 atgcaaattc ttttaataat ttttaaaaga tttattcaat tatgtataca gtgttctgcc
    20641 tgcatgtatg tctacaggcc agaagagggt attggatccc attacaggta ggtgtgagcc
    20701 accatgtggt tgttgggaat tgaactcagg acctctggga gagcagacag tgctcttaac
    20761 ctctgagccc ccacatgcta actcttaact gtgtcctgtt cacttttcat cttcattgcc
    20821 cctattggat aagtgattct ttgatttacc ta
//