LOCUS       240379#pL1L2_GT2_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21441 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT2_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 240379
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000164782
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            6925..7241
                     /db_xref="ENSEMBL:ENSMUSE00000907105"
                     /rank="1"
                     /note="ENSMUSE00000907105"
     exon            7322..7375
                     /db_xref="ENSEMBL:ENSMUSE00000614672"
                     /rank="2"
                     /note="ENSMUSE00000614672"
     exon            8919..9075
                     /db_xref="ENSEMBL:ENSMUSE00000532356"
                     /rank="3"
                     /note="ENSMUSE00000532356"
     misc_feature    4275..9636
                     /note="5 arm"
     misc_feature    9664..9688
                     /note="B1 Gateway"
     misc_feature    9701..9748
                     /note="Frt"
     misc_feature    9749..10248
                     /note="mouse En2 intron"
     primer          complement(9817..9838)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9713..9736)
                     /note="FRT3"
     primer          13907..13925
                     /note="seq primer NF"
     misc_feature    13967..13974
                     /note="RE sites"
     misc_feature    15767..15800
                     /note="loxP"
     misc_feature    complement(13975..14006)
                     /note="Rox"
     misc_feature    complement(15727..15758)
                     /note="Rox"
     misc_feature    14007..14028
                     /note="polylinker"
     CDS             14587..15183
                     /note="puromycin N-acetyl transferase"
     misc_feature    14702..14707
                     /note="Mutated RsrII site"
     intron          10249..10623
                     /note="mouse En2 intron"
     misc_feature    10621..10623
                     /note="Splice Acceptor"
     exon            10624..10670
                     /note="mouse En2 exon"
     primer          10524..10545
                     /note="seq primer FCHK2"
     misc_feature    10671..10793
                     /note="1Dleader"
     misc_feature    10794..10845
                     /note="F2A"
     misc_feature    10941..11657
                     /note="EGFP"
     misc_feature    10851..10940
                     /note="3X NLS"
     misc_feature    13723..13961
                     /note="SV40 polyadenylation site"
     misc_feature    12756..13700
                     /note="ERT2"
     promoter        14039..14550
                     /note="PGK promoter"
     misc_feature    15294..15726
                     /note="PGK pA"
     primer          13781..13804
                     /note="LRPCR RAF5"
     misc_feature    11667..11720
                     /note="T2A"
     misc_feature    11717..11717
                     /note="A to G to kill RsRII site"
     misc_feature    11721..12746
                     /note="Cre"
     misc_feature    10639..10671
                     /note="FCHK lf2a_lacz_gt2"
     misc_feature    10639..10654
                     /note="FCHK QC prefix region"
     misc_feature    10655..10665
                     /note="FCHK QC critical region"
     misc_feature    10666..10671
                     /note="FCHK QC suffix region"
     misc_feature    15823..15843
                     /note="B2 Gateway"
     misc_feature    9637..15873
                     /note="Synthetic Cassette"
     exon            16253..16386
                     /db_xref="ENSEMBL:ENSMUSE00000614680"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000614680"
     exon            16824..16886
                     /db_xref="ENSEMBL:ENSMUSE00000614679"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 2 ENSMUSE00000614679"
     misc_feature    15874..17065
                     /note="Critical Region"
     misc_feature    17089..17122
                     /note="Downstream LoxP"
     misc_feature    17066..17145
                     /note="synthetic loxP region"
     exon            17894..17938
                     /db_xref="ENSEMBL:ENSMUSE00000614678"
                     /rank="6"
                     /note="ENSMUSE00000614678"
     exon            18018..18165
                     /db_xref="ENSEMBL:ENSMUSE00000532352"
                     /rank="7"
                     /note="ENSMUSE00000532352"
     misc_feature    17146..21441
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatttact cattttgttc attatagagt gtgggcgcgg gtgtgagtgt
     4321 ataagctaca gctcccacct ctggagatca gaggacaact ctgcagccag ttctctccct
     4381 ccatctttat gtgggtcacc tagggttaaa ttctggtggc caggcttgca gaaccagcac
     4441 ctttctgtgc tgagccattt cactaccact ggacattgtt tttaaacaac tataatttgt
     4501 gtttcatcat ggaaacatag ttgacacata gaaatagaat ctcaatgaaa aagtgaaaag
     4561 ccagtgaaag attaagaatt attaatttct ctgctgacat tgaattaagt attgtgtatc
     4621 ttgggctatg cattagaatc atccagagag gtttttaaat gctctgtccc catcccagag
     4681 caaaaggatc agaatctctt gagggatgga aaactcccgc taccatacaa atcagtgctc
     4741 cccaagtggt ccccacaaca acctagttag gagccagctg ctctctggtt tttggttttg
     4801 tttgtttgtt gatattaaat ggtctttgtt agagcaagat aactagcaat cctggacaat
     4861 gctctgaaat atcaccgaac tgaatataaa tttgtctttt cagtcaagag ccagatctgc
     4921 ctctccctct ggctctgaag accggtctct aaaagcacat atttttccct ccatatattt
     4981 atggtgaaca tgaaaaacaa actaactgag gaaaagaaat atcacccgga ctcctccacc
     5041 ctccttggtt ggtctttcca aaagaataaa aataaataaa gacttaactt ctcttaaatg
     5101 tttcaaaaca aaataattaa aatgcctggg taaacagcca tgaaacttaa tgagctattt
     5161 cctccatttc ttaagtcaca gactcagggt ctatcatgga aatagctgca ggaatttgat
     5221 tctgccagat catggtggac tggcatcagg gactgtgccc tgcagggact ctttctctgc
     5281 cttgcacccc atttgaccat gctgcctgag ttgctgccta gcaactaatt atacaagatc
     5341 acgacaattt cccgggcctg actggaggaa cctgtccaac cctggcgtag gacacacaaa
     5401 gaacaatcat gaaaaggaca gctttaattg tcttcatctt caatttttct tccaagaaaa
     5461 ctatcaagga tggttaggta gactcctgag aaaatcacaa ttctctcatg aaagagatca
     5521 aatctttcaa gcagccatga aaaagaaaaa gaaacaccga gtacttccca aatcaccgac
     5581 caagcaattt tgtcccttct cagaagacag ccaagtgata aaagaaatgc ctggtaaaac
     5641 cacggaggca ttctgtcagc ccaggcaagg gctgttcagc tgacgcacac ttcagcatag
     5701 tcacacctcc acatcatgca ctagtgtagg actccacgta cataaatgtc caagtctata
     5761 gggagaggaa gtggactgct gaggtcatgt ggtggagagg aaagggcgtg ccggctcttc
     5821 accaggaggc atcattttgg atgtgattga atattctgaa attgatctac ggtgaggatc
     5881 ataaactttc tagaagtcac tgaatcctgt cttttaaatg gatggattct atgacataaa
     5941 ttatagctga ataaagctac atttaaaaca ggtattgtaa ccaggtgtga ttggtccatc
     6001 cgtggcactg cggaaggctg aggcaggata caatgaattc aaagtcatct tagactacct
     6061 taaaaccatg gctcaaaaaa ccaaaatcaa aacaacaaca acaacaacaa caaaaaccta
     6121 aagcaggggc tgtccaggtc tgaaattcct ctgctacctt gcaggtctgt catgaacacg
     6181 ccctatgtgt tttgttcttt ctctatgccc atcatgaaaa gttgctataa ttgaaatgcg
     6241 gagcacatac taccacatct aaagttccat agaatcacca gatttgagac tagaaacaca
     6301 aacaaggaat ctttcctaat ctggagaaag aggaagaaaa aaagaaatgt aactattgtc
     6361 tggaggttag gagcactcta caatgtacct actctttaca acaaatgacc catcagggaa
     6421 aaccgttttc cacctgaaaa taagtccaga ggccgagccc ctaagacaag ttttccataa
     6481 catttatcaa atgctgcttg gttttacttt taaagtaggc actgttcccc acggggcgcg
     6541 aagctgggaa gttttgttct cagaaccggg tggcttggct gctctgcacc ctgctgagcc
     6601 cctcaattcc ctcaaggcgg ctcctaagac cgtctctgtc cagtaccctg cacgccctaa
     6661 gtgtccctag gcgtttcccc aaacgcgctc cgacgccttt tcccatcaag tccccggaca
     6721 ccacaggcga ccttcacgcc tccccagaga accccacacc gcaggaagag gcgccagtcc
     6781 gctggcctcc cccggggacc gtcggggggg gggggcgagg gaggagctgg ccggccaccg
     6841 ccctacgggg gcgggagggg gagggctggg gaccgctcct agcccctgct ggcgcccccc
     6901 gcacagtgct catgcgcgcc gcccgtcttt atagcggcca cggagccgcg cttgccgcac
     6961 tcgggtgtag ccgggcggat cccacgcagg tgcgggaccc ggggaggtgc atagggtctg
     7021 tgcctctgcc tgtgtgtcct ggcgtacccc acgctggtcc gaggtggcac agcgcgtctg
     7081 gagctgctcg aactgcagcg gtcgggttct cccgccttcg ccggcttccg aggagcgcag
     7141 ggccgtgcgg tccccggcga acctcgttca cgcctcactc tcccgcaggt ccacggagat
     7201 cctcgccatg ccctcgtcca ggatccctgc tctctgcctc ggtgggtaca ctcccatccc
     7261 ctacccctcg ccctctcgcg cgggcggagg ctgtagccac ctcgctgtcc tctctccgca
     7321 ggtgcgtggc tgctgctgct gctgctgccc cggttcgcgc gcgccgaggg agcgggtgag
     7381 ttaggtgcgg gaggagggag atggggagga cagggtcagt tcccgcaggc tagggacctg
     7441 cgtagggatg cggggctgca gaggcggaga cccgccctca cccatctcca gcgcagccct
     7501 cgggcttgcc tgtttttcca cgccagggtc cccggggtcc gtttgtgcct ccttcccggg
     7561 agcacgccac ctagccccgg gaccattcgt cccgagttac tccgttgttt tccgggctgg
     7621 ggacaactgt ttggtcccag cccacagagg agggctgctc acatcctggt cccacctaat
     7681 ccgatcacct cgctgtgctg tgcgtttgtg cttgtaaccc ctttggaaaa cagcgtcggc
     7741 ggttcctata tctatctccc tgggagcctc aaaagagttt gttccacgtg tagaagtccg
     7801 agtgtggttc ggtgtctttt ctgcgatgta agagagtttc gcttctaaaa tcttacgctt
     7861 gcttttcttc aaagtagaaa tggtaagtac cttttgccca acaaagcaag tgttagcagc
     7921 tccggacaat ctcggtcctg gattccagag tcgttgtcgc cccactccca cctaccccca
     7981 cccccgtcgc tgaaaaggga aaagatttgg gttatttcat agagttgcaa ggctcctatt
     8041 tgaagttagg attttaaccg agcctgaaga attgatattg caccttgcgt cttccctttt
     8101 cttccgtggt ccaggcaagg atccttggca gaggcaaatt actcaacttt taaaaccatc
     8161 ctttcttgcc tcgtgggaga gacttttttt ttttttaaat tggtccgact gactgtaaga
     8221 tttaagcata ttagcagtgc tttcttttaa aaaaaaaaag ggggggggtg ctgccttcca
     8281 tattcctttg gacagatctg gaggagatac aatcagattc aagtagagga ctaatttcct
     8341 tgtgtcctag cctttgggat ttgcggcgtg ctccgtaggc taattaactc tatgactttc
     8401 atttcctcaa ttgtaaaata tatgatccag gcacctgtta actggttggt ttatgtgaat
     8461 gtgggactgt aaagccctga ctgtatcagg taatgggcca gacagccccc tgggaagggt
     8521 agctgaagac gtttgcattt gtctatgaag acactccaca aaggctcaat acagatgtac
     8581 cgtcatcata aataactgga aagagttcat cacaattaag atcttttttt atttctaggt
     8641 caaagactaa tgctattttc tcattctgat gcactttttc aattacattt tccaaattga
     8701 tgaagaactg gaaacaatgt cacattagat atctgtagta acttggccct agattaaatt
     8761 ggaaacccct ttgggcttgc taatatctaa aacggtgtca acatttaagt cggtgttttg
     8821 gcagaagtat agaaattggg ggaataaata gaatccctgc aatacttccc ccgtaaagca
     8881 gcccaggagc tcttacagga cacacccttt ctcttcagtt cccattcctg tcacctgctt
     8941 taccagaggc ctggatatcc gaaaagagaa agcagatgtt ctctgcccag gaggctgctc
     9001 tcttgaggaa ttctctgtgt ttgggaacat agtgtatgcg tcagtgtcca gcatctgcgg
     9061 cgctgctgtc cataggtaag ccggagccca cgggaagggc gtggcggtgc caactggcac
     9121 acgtgtgatt gtttcctttc caacttaagc tgtgtggacc cctctcctac ctgcttgctt
     9181 cttcttggtg ccaattttgg ccatagtttt gagattttcg tgagtattcc tgcatgacct
     9241 aatgtgcaag ggaaactttc tgacaatgag cagagtacat tgttttttgt tgttttgttt
     9301 gtttgtttgt gtgcgtgcgt gctcacgggc acccatgtgc acccatggag gactgttgag
     9361 agggtttgaa ggtggaatta catgtgtttg tgaattacct cacagtgcta tgaactgaac
     9421 ttggggcttc tggaagagca gccagctctc ttaaacaccg agccattcct ccagcccagc
     9481 attagtgcgc gatggccaca tttctataat tctaaaatat tcctcatcgt gtcatatata
     9541 gtagggtttt ttatactaca caccctgtcg ttttaacttc caccacaagt cttacagtag
     9601 acacagcctt gttctggttt tcacatgaga ctgaacaagg cgcataacga taccacgata
     9661 tcaacaagtt tgtacaaaaa agcaggctgg cgccggaacc gaagttccta ttccgaagtt
     9721 cctattctct agaaagtata ggaacttcga accctttccc acaccaccct ccacacttgc
     9781 cccaaacact gccaactatg taggaggaag gggttgggac taacagaaga acccgttgtg
     9841 gggaagctgt tgggagggtc actttatgtt cttgcccaag gtcagttggg tggcctgctt
     9901 ctgatgaggt ggtcccaagg tctggggtag aaggtgagag ggacaggcca ccaaggtcag
     9961 cccccccccc ctatcccata ggagccaggt ccctctcctg gacaggaaga ctgaagggga
    10021 gatgccagag actcagtgaa gcctggggta ccctattgga gtccttcaag gaaacaaact
    10081 tggcctcacc aggcctcagc cttggctcct cctgggaact ctactgccct tgggatcccc
    10141 ttgtagttgt gggttacata ggaaggggga cgggattccc cttgactggc tagcctactc
    10201 ttttcttcag tcttctccat ctcctctcac ctgtctctcg accctttccc taggatagac
    10261 ttggaaaaag ataaggggag aaaacaaatg caaacgaggc cagaaagatt ttggctgggc
    10321 attccttccg ctagctttta ttgggatccc ctagtttgtg ataggccttt tagctacatc
    10381 tgccaatcca tctcattttc acacacacac acaccacttt ccttctggtc agtgggcaca
    10441 tgtccagcct caagtttata tcaccacccc caatgcccaa cacttgtatg gccttgggcg
    10501 ggtcatcccc ccccccaccc ccagtatctg caacctcaag ctagcttggg tgcgttggtt
    10561 gtggataagt agctagactc cagcaaccag taacctctgc cctttctcct ccatgacaac
    10621 caggctccca ggtcccgaaa accaaagaag aagaacgcag atcgcagatc tctagagtca
    10681 ccgagttgct ttaccggatg aagagggccg aaacatactg tccaaggccc ttgctggcaa
    10741 tccacccaac tgaagccaga cacaaacaga aaattgtggc accggtgaaa cagactttga
    10801 attttgacct tctcaagttg gcgggagacg tcgagtccaa ccccggaccg gtgcatgtgg
    10861 accccaagaa aaagcggaag gtggacccta aaaagaaacg caaagtggat cctaagaaaa
    10921 aaagaaaggt gcacgtgtcc atggtgtcca agggcgagga actgttcacc ggcgtggtgc
    10981 ccatcctggt ggaactggat ggcgacgtga acggccacaa gttcagcgtg tccggcgagg
    11041 gcgaaggcga cgccacatac ggaaagctga ccctgaagtt catctgcacc accggcaagc
    11101 tgcccgtgcc ttggcctacc ctcgtgacca cactgaccta cggcgtgcag tgcttcagca
    11161 gataccccga ccatatgaag cagcacgact tcttcaagag cgccatgccc gagggctacg
    11221 tgcaggaaag aaccatcttc tttaaggacg acggcaacta caagaccagg gccgaagtga
    11281 agttcgaggg cgacaccctc gtgaacagaa tcgagctgaa gggcatcgac ttcaaagagg
    11341 acggcaacat cctgggccac aagctggagt acaactacaa cagccacaac gtgtacatca
    11401 tggccgacaa gcagaaaaac ggcatcaaag tgaacttcaa gatccggcac aacatcgagg
    11461 acggctccgt gcagctggcc gaccactacc agcagaacac ccctatcggc gacggccctg
    11521 tgctgctgcc tgacaaccac tacctgagca cccagagcgc cctgagcaag gaccccaacg
    11581 agaagaggga ccacatggtg ctgctggaat tcgtgaccgc cgctggcatc accctgggca
    11641 tggacgagct gtacaagcct gcaggcgagg gcagaggaag tcttctaaca tgcggtgacg
    11701 tggaggagaa tcccgggccg tccaatttac tgaccgtaca ccaaaatttg cctgcattac
    11761 cggtcgatgc aacgagtgat gaggttcgca agaacctgat ggacatgttc agggatcgcc
    11821 aggcgttttc tgagcatacc tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat
    11881 ggtgcaagtt gaataaccgg aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc
    11941 ttctatatct tcaggcgcgc ggtctggcag taaaaactat ccagcaacat ttgggccagc
    12001 taaacatgct tcatcgtcgg tctgggctgc cacgaccaag tgacagcaat gctgtttcac
    12061 tggttatgcg gcggatccga aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc
    12121 tagcgttcga acgcactgat ttcgaccagg ttcgttcact catggaaaat agcgaccgct
    12181 gccaggatat aaggaatctg gcatttctgg ggattgctta taacaccctg ttacgtatag
    12241 ccgaaattgc caggatcagg gttaaagata tctcacgtac tgacggtggg agaatgttaa
    12301 tccatattgg cagaacgaaa acgctggtta gcaccgcagg tgtagagaag gcacttagcc
    12361 tgggggtaac taaactggtc gagcgatgga tttccgtctc tggtgtagct gatgatccga
    12421 ataactacct gttttgccgg gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc
    12481 agctatcaac tcgcgccctg gaagggattt ttgaagcaac tcatcgattg atttacggcg
    12541 ctaaggatga ctctggtcag agatacctgg cctggtctgg acacagtgcc cgtgtcggag
    12601 ccgcgcgaga tatggcccgc gctggagttt caataccgga gatcatgcaa gctggtggct
    12661 ggaccaatgt aaatattgtc atgaactata tccgtaacct ggatagtgaa acaggggcaa
    12721 tggtgcgcct gctggaagat ggcgatctcg agccatctgc tggagacatg agagctgcca
    12781 acctttggcc aagcccgctc atgatcaaac gctctaagaa gaacagcctg gccttgtccc
    12841 tgacggccga ccagatggtc agtgccttgt tggatgctga gccccccata ctctattccg
    12901 agtatgatcc taccagaccc ttcagtgaag cttcgatgat gggcttactg accaacctgg
    12961 cagacaggga gctggttcac atgatcaact gggcgaagag ggtgccaggc tttgtggatt
    13021 tgaccctcca tgatcaggtc caccttctag aatgtgcctg gctagagatc ctgatgattg
    13081 gtctcgtctg gcgctccatg gagcacccag tgaagctact gtttgctcct aacttgctct
    13141 tggacaggaa ccagggaaaa tgtgtagagg gcatggtgga gatcttcgac atgctgctgg
    13201 ctacatcatc tcggttccgc atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat
    13261 ctattatttt gcttaattct ggagtgtaca catttctgtc cagcaccctg aagtctctgg
    13321 aagagaagga ccatatccac cgagtcctgg acaagatcac agacactttg atccacctga
    13381 tggccaaggc aggcctgacc ctgcagcagc agcaccagcg gctggcccag ctcctcctca
    13441 tcctctccca catcaggcac atgagtaaca aaggcatgga gcatctgtac agcatgaagt
    13501 gcaagaacgt ggtgcccctc tatgacctgc tgctggaggc ggcggacgcc caccgcctac
    13561 atgcgcccac tagccgtgga ggggcatccg tggaggagac ggaccaaagc cacttggcca
    13621 ctgcgggctc tacttcatcg cattccttgc aaaagtatta catcacgggg gaggcagagg
    13681 gtttccctgc cacagcttga tgaagatctg agctcttaat taatgatcat aatcagccat
    13741 atcacatctg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
    13801 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
    13861 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    13921 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgttt aaactaactt
    13981 taaataattg gcattattta aagttacata tggcggccgc cctaaggctc gacctcgaaa
    14041 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
    14101 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
    14161 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
    14221 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
    14281 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
    14341 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
    14401 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
    14461 aggtcttccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
    14521 tcctcttcct catctccggg cctttcgacc tgcatccatc tagatctcga gcagctgaag
    14581 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
    14641 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
    14701 ccagatcgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
    14761 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
    14821 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
    14881 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
    14941 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
    15001 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
    15061 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
    15121 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
    15181 gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca
    15241 tcgatgatat cagatccccg ggatgcagaa attgatgatc tattaaacaa taaagatgtc
    15301 cactaaaatg gaagtttttc ctgtcatact ttgttaagaa gggtgagaac agagtaccta
    15361 cattttgaat ggaaggattg gagctacggg ggtgggggtg gggtgggatt agataaatgc
    15421 ntgctcttta ctgaaggctc tttactattg ctttatgata atgtttcata gttggatatc
    15481 ataatttaaa caagcaaaac caaattaagg gccagctcat tcctcccact catgatctat
    15541 agatctatag atctctcgtg ggatcattgt ttttctcttg attcccactt tgtggttcta
    15601 agtactgtgg tttccaaatg tgtcagtttc atagcctgaa gaacgagatc agcagcctct
    15661 gttccacata cacttcattc tcagtattgt tttgccaagt tctaattcca tcagaagctg
    15721 gtcgagtaac tttaaataat tggcattatt taaagttagg cgcgccataa cttcgtatag
    15781 catacattat acgaagttat gtcgagatat ttaaatatta gacccagctt tcttgtacaa
    15841 agtggttgat atctctatag tcgcagtagg cggaatatct tgctgatgtc actctggtaa
    15901 tccaatacca tgccatccta cagtagaggc agggtatgat tcttttttta aatacaggtc
    15961 aagggcacat gtggttaact ctttaagaaa tttcatgcct ctttattaag tctgaacctc
    16021 tgaaatgtag gaaacaggat atcagtgtat tcttatcacc ccttttaaaa agtttgacaa
    16081 tacaaagtca ttttcccaga catacttggt tctgtgtaag caggtcttca agggccagca
    16141 gcaagcatcc ttaggcaact aacttcgcca cccagctgtt ttcttgacca atgtaagcaa
    16201 cagatggcta tgtctattgg gtagtgtctc agctgtaatt cttgcctggc aggggagtga
    16261 ttggcacctc agggggacct gtgcgtgtct acagccttcc tggtcgagag aactactcct
    16321 cggtagatgc caacggcatc cagtctcaga tgctttcccg atggtccgcg tccttcgctg
    16381 tgaccagtag gtacaagttc attgttttca gccctgtata aacaccgcct ccactctcct
    16441 caagagggtt ctctgctttt cttagcccca cctttttcat tctgttggtt aagaagtcaa
    16501 ccatgggaca gagtcaccca aaccaaacgg gggtctggga gtgttaacaa tcccacttta
    16561 cacggtggaa gccaaaatct gaattgtttg caatctggat gcgtagatac ttagcgagga
    16621 actaactgtc aaagtaggca ttcagctttg ggtgcttttc aaatacatca agaacgcatt
    16681 ttgattctcc aaacctaagg gtcagtgtga tgtttatacc cgagtcaaca ttactccctt
    16741 aagtgtcatg gagaacttta gattcataat gtcctaatgt cagaaaagga gggagaaaaa
    16801 caattaactt ctcatttcta cagaaggcaa aagcagtacc caggaagcca caggacgggc
    16861 agtgtccaca gcccacccac cttcaggtag gcaacactgg gctgatcccc ttgttacctg
    16921 gagtttgtgg atgctttcct tcagggcact aacgagaagc aaagcagcta cctcatattt
    16981 actgatgtat tcatggatgg aagtattcta ctttatattt cacagttctt ctttattata
    17041 acctaagatg gaagtattct actttgagat ggcgcaacgc aattaatgat aacttcgtat
    17101 agcatacatt atacgaagtt atggtctgag ctcgccatca gttcaataac ctaagtcagc
    17161 cattctagga ggttctttct atctctccct gctttgatgg accatgagga gaaactggtc
    17221 agagaccact ttccggctgt ttgcttcatg aaatcccctt agtttctgca ctgttatgtg
    17281 attgaaagaa atcggtgtgt gtctgctacc aaaaaaaaaa aaaaaaaaaa aaatccaggg
    17341 atatcttttt ctaaaatagt gaccattttg ttttatttta ttagagattg attttatgtg
    17401 tatatgtgtg tgcttgaatg tatttatgtg catcgtgtgt gtgcaggcaa aggaagaacc
    17461 aaaagaggat agtagctatt ttggagccta agttacaggt tattgtaact tgagcagctt
    17521 aatgtgagtg actggaacca aacctgagtc ttctgtaaga gccgtgagca ctcttgcatg
    17581 ctattccatc tctctgaccc tggcagtggc cattttaaat tcaaagacat tataagcatg
    17641 tggctccgta ttcccaatct tgccttaatt gctggtttaa gatatcaaag ccttggatag
    17701 attcatacca agtacatagg cctgccacca ttctgctgtg tttctttgtt ggagtattgc
    17761 tttcctagcc caaaagacca tgaaaagaac ttaaggtctt tctaagaatg gcaccccttg
    17821 ccatgggctc acccaggtgg aagttcctgc tgtgtaagca tcttgttgtt gccaagagct
    17881 tccttctttt caggtaaaag actaaagaag acaccagaga agaagactgg caacaaaggt
    17941 aagctaattg ctcggggaaa tggcaggtcc ttgaggtgct cgggggacta acgaagccct
    18001 tctctgcctc tctgtagact gtaaggcaga cattgcattt ctcattgatg gaagcttcaa
    18061 tattgggcag cgccgattta atttgcagaa gaattttgtt gggaaagtgg cactaatgtt
    18121 gggaattgga acagaaggac cacacgtggg tctcgttcaa gccaggtacc aactttaaag
    18181 agattatgga tatcttcaat gatgacagct taccaagctg ttatattcat ttcagtaaac
    18241 ataacttaaa tctggacaag ctgtaatcca aactataaac tcaggattta agaatgccta
    18301 tgcatggctg gacatgatgg tgtatacctt taatctcagc acttaggcag aggcaggcaa
    18361 ttgcctatgt cagctttcta ctcatctctg tgagttcaag gccagcctgg gctatctagc
    18421 aaataggcca gccagggctg catagtgagt cccagtcttt aaaaataaaa taaaatctgc
    18481 atgtgtatca aaagatggcc tagtcggcca tcactggaaa gagaggccca ttgaacttgc
    18541 aaactttata tacctcagta caggggaaca ccaaggccaa aaagtgggaa tgggtgggta
    18601 ggagagtggg tgggagggta tgggggactt ttgggatagc atgggaaatg taaatgagga
    18661 aaatacctaa taaaaaagaa aaaagggaaa aaaataaaat aaaataaaaa tctctgaaag
    18721 cacttgcatg cacatgcata attctcaatc cccaacactg caaacaacaa catcatcaaa
    18781 gactgcctgg gtagactgtg cagggctagt gtgtcattgg cataaaacga cacctttctc
    18841 tgcttcactc agttcttggg ttttcctttt gagagaaaga agccataaac tgtgttccct
    18901 gcctttttgt tgatttgttt gttagcttgc ttgcttgttt gttttgtttg ccctttctat
    18961 gtttcttcct tgggatccaa gcatggctgt tacactctag ttggcagtgt gaacctggga
    19021 gagtggagcc tgaaaaaaaa aaaagaatga ggtagactag agtctcatcc agtaggcaac
    19081 tttattcagg gtatcagaca atttataatc tgagggtact ctgaggatta agcaaggtcg
    19141 tatgagcaaa gttcactttg agttcaagct atatccagtc acaacggcaa tgtcacattg
    19201 agtttaggct gtatacagtc atgagggtgt acagcttaca aatgtcattc tgaataataa
    19261 caggtaaaat gatggctaat ctaaagtaca acccattcct gtgattatac ctgggagagg
    19321 catgaaagca ggttccaaga acagcccata tgatggagga acagagatca cagcctctgt
    19381 cttgcttacg tggaattttc taagaagctc tcagaatctc ctcacagagc ttcattcatg
    19441 gatcatgttc tgacacatga cctcttgcac tttatgactg taaaaataat atttgtgggt
    19501 tttagctgtg tactatgttc cttacagaag gtttccaaag tggggtacaa agaaataaca
    19561 tttctcagac caagccttta taaagttgtt tcctttctga atttgagaag atgtttgtac
    19621 gcatgctccc ctttctacaa ttcaggctac caaaatgttg gaccctgtgt gttttccccc
    19681 ttagcatttt cttgaggcaa atgtgcattg tagattgctt tatagttttc ctttgcatac
    19741 ttgtgtagac attcttccct cggctttcat ggttacacag tgccctgtga ggagaagcct
    19801 tctgtctgtt ctctgtccat ctttgattac ttcattcacc tgaattcctg gatgtgacgt
    19861 tcagcacttt caaagtctct gataaaatga ctaagttgtt ttgtagaaag ttgagcagct
    19921 aatactatca gtggtgtgta gccccgcaca accacaaaca ttaatattct gcttgttacc
    19981 tttcttgttg tttgtttttc aagacagaat ttctctttgt agccttggct gtcctgggat
    20041 ttgttctgta gaccaggctg gccttgagtg cgagagatca ctctgccgct gccttctaag
    20101 tctgcttgtt actttttata gttggctctg cttagaagtt atttaacttc tctgtataat
    20161 tcagtcattt ccccgtgtct ttaaaacact gagctctttg ttgttttgac ttaaaatgaa
    20221 atatcaattt cataaacact tagacaagtt tctttgactc agctgctgat tttgctggtt
    20281 ggctggtttt ttattttgtt tttggtttgt tttgttttat agaccagggt ggccttgaac
    20341 tcaagaggtc tgtctgcctc tgcctcccag gtgctgggat tacaggcatg ctactatgtc
    20401 tggcttatgt gcacaggtac atatacatgt gtatatagaa ggtagaggac cacctcaggt
    20461 cttattcttc aagtgtcatt cactttctgt tgttttaaac aggtatctca taggcctgga
    20521 actagccgag taggctaagg tggctggcca ccatgtccag ggacctgact cccagcacta
    20581 ggatcactag tctaggccat cacactagct ctctttacat gggatctggg gataaaccca
    20641 ggtcctggac tgaaccagga cctgtcctca acatggtctc taatgaacaa aatatagttt
    20701 aaagaactgg aattctttat cataactagg attggcaaat gtcattgttt ataatgaatt
    20761 cccttgtacc ctacccttaa tcttagggct ggaaatggct cagcgattaa gagcactggt
    20821 ccctcttcca aatgacacag gttcacttac atggttgctc acatatatct gtcattccag
    20881 ttccaggaca tccaacatct ggcctctgta gacatcaggt atccacatgg tgcactgata
    20941 taacatgcaa gcaaaacacc catacacata caaataataa aatgtgtttt gaccagagag
    21001 acttaggcca ataaattgct gatgtagtct catatccagt tgtttcctat ctacttggtg
    21061 cccaactgta cagacttcca ctaaagctgg agcccggagc cgcacctgct ttaacacact
    21121 gcatacacgt agtctgttat tccagtccca acctagattt tggaatgatt tttgagccat
    21181 ttgagtctga atttaaaagg cagccatgtt ttcttaggca tattatctaa gtgttctgag
    21241 cctgtttctt taaataattg ggtaagtcta ccttgtgaaa gtatgaagtg aaattgcata
    21301 tacttagcta tcacccaaaa tataataaac gctgaatata gcaactgtcc ttgctacatt
    21361 tctgcagcat tcccagtgag tggaagcgat gtcctagttg actatatact atgtgattta
    21421 aaaagacttg ggtctgactt t
//