LOCUS       KO-first_condition_ready_125162:124937_MGI:104861        20963 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Bcat1 targeting project(s): 125162:124937
ACCESSION   unknown
SOURCE      Mus musculus.
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 262868
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..9876
                     /note="5 arm"
     misc_feature    9904..9928
                     /note="B1 Gateway"
     misc_feature    9941..9988
                     /note="Frt"
     misc_feature    9989..10488
                     /note="mouse En2 intron"
     primer          complement(10057..10078)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9953..9976)
                     /note="FRT3"
     primer          14140..14158
                     /note="seq primer NF"
     misc_feature    14200..14207
                     /note="RE sites"
     misc_feature    16000..16033
                     /note="loxP"
     misc_feature    complement(14208..14239)
                     /note="Rox"
     misc_feature    complement(15960..15991)
                     /note="Rox"
     misc_feature    14240..14261
                     /note="polylinker"
     CDS             14820..15416
                     /note="puromycin N-acetyl transferase"
     misc_feature    14935..14940
                     /note="Mutated RsrII site"
     intron          10489..10863
                     /note="mouse En2 intron"
     misc_feature    10861..10863
                     /note="Splice Acceptor"
     exon            10864..10903
                     /note="mouse En2 exon"
     primer          10764..10785
                     /note="seq primer FCHK2"
     misc_feature    10904..11026
                     /note="1Dleader"
     misc_feature    11027..11078
                     /note="F2A"
     misc_feature    11174..11890
                     /note="EGFP"
     misc_feature    11084..11173
                     /note="3X NLS"
     misc_feature    13956..14194
                     /note="SV40 polyadenylation site"
     misc_feature    12989..13933
                     /note="ERT2"
     promoter        14272..14783
                     /note="PGK promoter"
     misc_feature    15527..15959
                     /note="PGK pA"
     primer          14014..14037
                     /note="LRPCR RAF5"
     misc_feature    11900..11953
                     /note="T2A"
     misc_feature    11950..11950
                     /note=" A to G to kill RsRII site"
     misc_feature    11954..12979
                     /note="Cre"
     misc_feature    10879..10904
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    10879..10894
                     /note="FCHK QC prefix region"
     misc_feature    10895..10898
                     /note="FCHK QC critical region"
     misc_feature    10899..10904
                     /note="FCHK QC suffix region"
     misc_feature    16056..16076
                     /note="B2 Gateway"
     misc_feature    9877..16106
                     /note="Synthetic Cassette"
     exon            16536..16699
                     /db_xref="ENSEMBL:ENSMUSE00001032948"
                     /type="targeted"
                     /note="target exon 1 ENSMUSE00001032948"
     misc_feature    16107..16769
                     /note="Critical Region"
     misc_feature    16793..16826
                     /note="Downstream LoxP"
     misc_feature    16770..16849
                     /note="synthetic loxP region"
     exon            20351..20493
                     /db_xref="ENSEMBL:ENSMUSE00001078362"
                     /note="ENSMUSE00001078362"
     misc_feature    16850..20963
                     /note="3 arm"
BASE COUNT     4939 a   5322 c   5329 g   5372 t     1 others
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggattgtct gcccagaagg gagagtttct gtaaattttt ctctatataa
     4321 ggacccaagt ctggcaggat ggctttaccc ttgtgacctc ccatgatgca ccaacctaaa
     4381 agcccctatg gctttggaca gtcataccag agctgtgatc ttactctctg cctgactgga
     4441 tttgcgatcc cctagcagcc attcctctga gtgagtctat gagggtgtta gcagagaggt
     4501 ttaactgagt agagaagacc tgtcctgaat gttgtggtac tgactcacgg gcttgggtcc
     4561 ctgactgaac aaaagggaaa cggtgttgag catcagcaaa catccctctc cctgctctct
     4621 gaccgtggac acagagacca gacacccaca tttcctatgc gattccttcc ttatagcagt
     4681 gagaaaagtc attaatgcag ggagtcaggg cttcaacggg gacttcagag gaggtaatag
     4741 aggtggggag ggcaggagag gacctggaag gtggagaggg aggagaattg aatgacaagt
     4801 catgctgtgc tgatcgggga tggagctcag gcagcagcaa gtgccttcag ggaaggtctt
     4861 gggtacttac tgtattgatt atttctatta ttattacatg tatgtgagtg ctttgtctgc
     4921 cttatggctg aggatgtcag gagagggttt tgaatccctg ggaactggaa ttacagcttc
     4981 tgtaaggcat atctcacttg ggtgctggaa ctgatattgg gtcctttaga accagcaggc
     5041 acccatagcc cacctaagca tctctccagc tctaagttgt tgctgctgtt cctcctcctc
     5101 ctcctcttct tcttccttct tctcctcctc ctcctcttct agacttattc agcccccaaa
     5161 tcttaggcag ctaaattggc cttgaccccc aatcctcctg cctcagcctc ccaagtgctg
     5221 agatgatagg caagagacat ggttccattt tctcatttaa ctctgaaagc ttttaaaaca
     5281 gaagtctctg aaagcaagcg agctggttgt cttcctctgg atccagccac cttattattt
     5341 gctttgttgg cttcggtcag aactgtcctc ccacttgcag gctgccctgc tccctgcctc
     5401 tgaggccaca ctgtagacta atggaatttc tcagttcact gactcctttc tgccaccctt
     5461 caaataactc gtgacatccc cccttctgtg aagggatttc tgggtgaccc agaaagaagt
     5521 agcctttgtt tctcaccaca cccaagcgcc atttgtctgt gcagaggact aatgggtctg
     5581 gttattgtga agcttagccc cgtctcccaa agctaaaaca aaaccaccac gctgaaatgt
     5641 tcctgagaca aacagacttg gcacaggcca tttgcagtgt ccaccggccg ctgacatcct
     5701 tattccctat ttagttatgt ggcttttctt ttagttaata agaagcacaa ctttatcatc
     5761 ccataacctt gtctgtcatt cggttggcat tacataataa aattcactta ataggagcac
     5821 aggatatgta aattgctttt cagggtagaa gaggagattg gagcaagggg ctaagtcagg
     5881 gatcctgagt gtatgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtttgttt gtggtagagg
     5941 gacaatgctt gtcactaaat ctttcttgac gcctagactg aattttgact cactcagtat
     6001 tgaagtggca tggagacttt ctccccactt gctctccaag gaacggcagt gagttctttg
     6061 aagatttgat ttttatttta catctatgag tgtcctatct gtgcacatgg gtgcagtgcc
     6121 catttgaggc cagaagaggg catcagatcc ctggaattga agttactgag attccacgtg
     6181 gttgctggaa actgaacctg ggtcctctgc aagaagagct gagccggtgc tccagccctc
     6241 aatgaattct tttcatggag agacaatgga tgtaagctat ggaaggttcc taagccatct
     6301 atggtccaca gaggaacttc gggcttgcaa tcatgagtgg acctggatta aattttagaa
     6361 aatacattcc tacgcacagc tttggccagc attaacaaat gctatttatt gtataactta
     6421 gctgttgcta aggatatgga tatgaatgaa gcgactcatc aagatgcttt gtcagcggga
     6481 gatttggtgg ctgtgtctaa ttctgcgtct ttccatctgt gctcgctgct gccccctagt
     6541 ggactaaatg caaactaact taccaaggtc aggaaagcac acgcacacag aggaattccc
     6601 ctttgcttct atactgtgtg ccctcacaga tcctaacgtc ccagcccgaa cgaggtgaaa
     6661 tttagtacag agaaaaaaaa ttattactct gtagctactg ttactttctt ttcttttctt
     6721 ttcttttctt ttcttttctt ttcttttctt ttcttttctt ttctcttctc ttctcttctc
     6781 ttctcttctc ttctcttctc ttctcttctt ttctttcttt ctttctttct ttcttttttt
     6841 tttttttttt gtataatgca attgtataaa atacttggtg tgtgtgcacc agcattcatg
     6901 aggtagggga caaagtacta ttaggttgta cataagacag acaattcatt cagaaattta
     6961 gaagctagat gtggccttca gataacagct agcatatcat atctgagtgg cctctctgga
     7021 ttccttatca tttgagttgg ttagtctcct gttgtgatcc tactgtgaac aaggcagctt
     7081 gcagagggag gggtttactt gggttggtga ttacagaggg ttagctcaca atggcagggg
     7141 aggaatgaca gatggcaggc aggaagccgg caaatcacat ccttaacctc gagcaaacgg
     7201 cagccgggtg agaccctcta gatgtgaggt gctgctacca gtgaaagtcc tccgggttct
     7261 tctcaaaaga aagacttcag aggcagttta tctagcaaag cagaatgtgc atttcaaaga
     7321 gagcctggag cagtatggcc cataatgagg agcggcccac tgagttctgg gttgtggatt
     7381 ttgaaacttt acattgcagt agtctatgct aattatacct aaggggtctc ctgacatttc
     7441 atacacgttt ataacatatt tggaccactc tcctcccatt aacccctttc cttttcctga
     7501 caagttgtgc tatttttaaa agttgtcttt atggttattt ataaggcagg tggcatattt
     7561 attggataaa gtgactcctt tccccctggg ttattgtgga tcaggtttcc ctttttaggg
     7621 tttgtcttcc tcccagcttc tagaaaagcc caaaagggaa gagagactga cagctacaat
     7681 gggagtgttt gacggggagc cgggggtctt tgcgccctca caccctcact ggtgtttgtg
     7741 tgtggcaact aggaaagtgt gccccctttc tctggctgcc tttgcttctg acacagcaga
     7801 aatatcctaa ctgcagcttc aaggcctcag agaggtgctc agagcaccag agacacagaa
     7861 gacgctgcaa cagcgacttc ctcagttatc tcctgtctga caccttgccc acacagtctc
     7921 accattgaag aaactctcag agacaagatg gttcccccag agctgggacg aaacaggtgt
     7981 ggatctgtgc acagtctccc tcccagatga gaagacatag agttcccaag ctcttcatcc
     8041 tgcaggggga gaagataagt tcctattgaa gaacaaacac ctttaatctc agcatctggg
     8101 aggcagaggc agggggatct ctgtgttgga ggccagcctg ggctatagag tgagttccat
     8161 gacagccagg attatgtaga gagactgtct caaaacaaaa accaaccaac caaccatatt
     8221 caaactagcc ccactgctct cagaccggtc tctctgactc agtcatgtag tttaccatca
     8281 gtttattcta ggggctcaga gtcctgcttg gtcctcctga tagagggagg tggggggctt
     8341 tcaggtgatg tccttaccca cagacagtgt gtgtgtgtat gtgctgtatg tgtatgtttg
     8401 tgcatgtgtg tgagtgtcaa tgtatgtgtg tgtgtgggtg tatgtggtgt gtgtatgtat
     8461 ctgagtgagt gtgtatatgt gagcatgaat gtgtgtgtat atgtgcatat atgtgtattt
     8521 gtgagtatgt gtatgtgtga gtgtgaatgt gttatatgtg tgtgagtgtg tgtgtgtttg
     8581 tgagcatgtg tgcttgtgtg tctatgtgtg tgagtgtgtg catgtatgtg tgtgaacatg
     8641 tgtgttggtg tgtgtatgtg tgtgtgcctc acgcacatgt gtcatggcac agtagtacag
     8701 tgtgcatgtc ggagggcagc tttcagaagt tgttttctca tctcacctct atgaggcagt
     8761 atctatctgc catgtcatga gctccaggct agcttgccca ccagctgctg gctgctctgt
     8821 cttcttccgg cctctctata ggagtgctgg gctcacacgt gccacatcct gccacatctg
     8881 gctccttccc atgttctggg gattcaactc aggcagtcaa gcatatgtgg caaacaagcc
     8941 ggttcactgg cccacaactg cttcctgggc ttcagctatt taccacctgg gtctaatgga
     9001 gagggcctga gggcaggcag tgcgtgctag aggcaggtgt ggtggagact caagcaaaag
     9061 gcagctgcag agtctgtcag catcagcttg ttaggcagag agcataaagt gtgcagccag
     9121 ctagagtcag ggccctcttc attgtcactg agcggctgcc agcctctgtg tcacaggtac
     9181 agaacactgc agtggtcagc atccatcctg ggggatcctg gcttgttggt ttgttcttgc
     9241 cgtcttacat agtatagctg aatggaactg acctgtatgt cttcatgaca cacggcctct
     9301 gggacttgga gtttctgata atccactgcc ctcacttcac gacttttcct tgtgctagag
     9361 aaatttatag tcttcctgta tttgtggaaa aggctgtctt ctgtcttgta tccatttgcc
     9421 aaagatttat ctttatttta tgcacatggc cgttttgcct gcttgtatgt gtgcacacac
     9481 gcacgcacag catagaggcc aggagagggc actggatccc ctggaagtgg atttatgaac
     9541 agctgtgagc ctccatgtag gtgctgtgaa ctgaacccgg gtcccctaca agagcagcca
     9601 gtgctagtaa ctgaaccacc tttccagttc cctgttgtcc atgatgtgaa gtctaagttc
     9661 tctcatctcc ttttgatggg acttctgctg tctctgcctg ccttctgttg tctattatgt
     9721 gctgcagagg caggcaccat cacagctggc ttccccatgc ccacctctgc tcatgggcct
     9781 caccttttct ggagtgccca gcaggcttca caaagacttg aagggggtgg gttatgttct
     9841 aggaggcagg ctcttcactc ttcctgtgag cagtttaagg cgcataacga taccacgata
     9901 tcaacaagtt tgtacaaaaa agcaggctgg cgccggaacc gaagttccta ttccgaagtt
     9961 cctattctct agaaagtata ggaacttcga accctttccc acaccaccct ccacacttgc
    10021 cccaaacact gccaactatg taggaggaag gggttgggac taacagaaga acccgttgtg
    10081 gggaagctgt tgggagggtc actttatgtt cttgcccaag gtcagttggg tggcctgctt
    10141 ctgatgaggt ggtcccaagg tctggggtag aaggtgagag ggacaggcca ccaaggtcag
    10201 cccccccccc ctatcccata ggagccaggt ccctctcctg gacaggaaga ctgaagggga
    10261 gatgccagag actcagtgaa gcctggggta ccctattgga gtccttcaag gaaacaaact
    10321 tggcctcacc aggcctcagc cttggctcct cctgggaact ctactgccct tgggatcccc
    10381 ttgtagttgt gggttacata ggaaggggga cgggattccc cttgactggc tagcctactc
    10441 ttttcttcag tcttctccat ctcctctcac ctgtctctcg accctttccc taggatagac
    10501 ttggaaaaag ataaggggag aaaacaaatg caaacgaggc cagaaagatt ttggctgggc
    10561 attccttccg ctagctttta ttgggatccc ctagtttgtg ataggccttt tagctacatc
    10621 tgccaatcca tctcattttc acacacacac acaccacttt ccttctggtc agtgggcaca
    10681 tgtccagcct caagtttata tcaccacccc caatgcccaa cacttgtatg gccttgggcg
    10741 ggtcatcccc ccccccaccc ccagtatctg caacctcaag ctagcttggg tgcgttggtt
    10801 gtggataagt agctagactc cagcaaccag taacctctgc cctttctcct ccatgacaac
    10861 caggctccca ggtcccgaaa accaaagaag aagaacgcag atctctagag tcaccgagtt
    10921 gctttaccgg atgaagaggg ccgaaacata ctgtccaagg cccttgctgg caatccaccc
    10981 aactgaagcc agacacaaac agaaaattgt ggcaccggtg aaacagactt tgaattttga
    11041 ccttctcaag ttggcgggag acgtcgagtc caaccccgga ccggtgcatg tggaccccaa
    11101 gaaaaagcgg aaggtggacc ctaaaaagaa acgcaaagtg gatcctaaga aaaaaagaaa
    11161 ggtgcacgtg tccatggtgt ccaagggcga ggaactgttc accggcgtgg tgcccatcct
    11221 ggtggaactg gatggcgacg tgaacggcca caagttcagc gtgtccggcg agggcgaagg
    11281 cgacgccaca tacggaaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
    11341 gccttggcct accctcgtga ccacactgac ctacggcgtg cagtgcttca gcagataccc
    11401 cgaccatatg aagcagcacg acttcttcaa gagcgccatg cccgagggct acgtgcagga
    11461 aagaaccatc ttctttaagg acgacggcaa ctacaagacc agggccgaag tgaagttcga
    11521 gggcgacacc ctcgtgaaca gaatcgagct gaagggcatc gacttcaaag aggacggcaa
    11581 catcctgggc cacaagctgg agtacaacta caacagccac aacgtgtaca tcatggccga
    11641 caagcagaaa aacggcatca aagtgaactt caagatccgg cacaacatcg aggacggctc
    11701 cgtgcagctg gccgaccact accagcagaa cacccctatc ggcgacggcc ctgtgctgct
    11761 gcctgacaac cactacctga gcacccagag cgccctgagc aaggacccca acgagaagag
    11821 ggaccacatg gtgctgctgg aattcgtgac cgccgctggc atcaccctgg gcatggacga
    11881 gctgtacaag cctgcaggcg agggcagagg aagtcttcta acatgcggtg acgtggagga
    11941 gaatcccggg ccgtccaatt tactgaccgt acaccaaaat ttgcctgcat taccggtcga
    12001 tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc gccaggcgtt
    12061 ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa
    12121 gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt atcttctata
    12181 tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc agctaaacat
    12241 gcttcatcgt cggtctgggc tgccacgacc aagtgacagc aatgctgttt cactggttat
    12301 gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg ctctagcgtt
    12361 cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgacc gctgccagga
    12421 tataaggaat ctggcatttc tggggattgc ttataacacc ctgttacgta tagccgaaat
    12481 tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt taatccatat
    12541 tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta gcctgggggt
    12601 aactaaactg gtcgagcgat ggatttccgt ctctggtgta gctgatgatc cgaataacta
    12661 cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca gccagctatc
    12721 aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg gcgctaagga
    12781 tgactctggt cagagatacc tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg
    12841 agatatggcc cgcgctggag tttcaatacc ggagatcatg caagctggtg gctggaccaa
    12901 tgtaaatatt gtcatgaact atatccgtaa cctggatagt gaaacagggg caatggtgcg
    12961 cctgctggaa gatggcgatc tcgagccatc tgctggagac atgagagctg ccaacctttg
    13021 gccaagcccg ctcatgatca aacgctctaa gaagaacagc ctggccttgt ccctgacggc
    13081 cgaccagatg gtcagtgcct tgttggatgc tgagcccccc atactctatt ccgagtatga
    13141 tcctaccaga cccttcagtg aagcttcgat gatgggctta ctgaccaacc tggcagacag
    13201 ggagctggtt cacatgatca actgggcgaa gagggtgcca ggctttgtgg atttgaccct
    13261 ccatgatcag gtccaccttc tagaatgtgc ctggctagag atcctgatga ttggtctcgt
    13321 ctggcgctcc atggagcacc cagtgaagct actgtttgct cctaacttgc tcttggacag
    13381 gaaccaggga aaatgtgtag agggcatggt ggagatcttc gacatgctgc tggctacatc
    13441 atctcggttc cgcatgatga atctgcaggg agaggagttt gtgtgcctca aatctattat
    13501 tttgcttaat tctggagtgt acacatttct gtccagcacc ctgaagtctc tggaagagaa
    13561 ggaccatatc caccgagtcc tggacaagat cacagacact ttgatccacc tgatggccaa
    13621 ggcaggcctg accctgcagc agcagcacca gcggctggcc cagctcctcc tcatcctctc
    13681 ccacatcagg cacatgagta acaaaggcat ggagcatctg tacagcatga agtgcaagaa
    13741 cgtggtgccc ctctatgacc tgctgctgga ggcggcggac gcccaccgcc tacatgcgcc
    13801 cactagccgt ggaggggcat ccgtggagga gacggaccaa agccacttgg ccactgcggg
    13861 ctctacttca tcgcattcct tgcaaaagta ttacatcacg ggggaggcag agggtttccc
    13921 tgccacagct tgatgaagat ctgagctctt aattaatgat cataatcagc catatcacat
    13981 ctgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
    14041 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
    14101 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    14161 tgtccaaact catcaatgta tcttatcatg tctggatccg tttaaactaa ctttaaataa
    14221 ttggcattat ttaaagttac atatggcggc cgccctaagg ctcgacctcg aaattctacc
    14281 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
    14341 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
    14401 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
    14461 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
    14521 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
    14581 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
    14641 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtctt
    14701 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
    14761 cctcatctcc gggcctttcg acctgcatcc atctagatct cgagcagctg aagcttacca
    14821 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
    14881 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccagatc
    14941 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
    15001 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
    15061 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
    15121 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
    15181 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
    15241 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
    15301 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
    15361 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
    15421 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcgatga
    15481 tatcagatcc ccgggatgca gaaattgatg atctattaaa caataaagat gtccactaaa
    15541 atggaagttt ttcctgtcat actttgttaa gaagggtgag aacagagtac ctacattttg
    15601 aatggaagga ttggagctac gggggtgggg gtggggtggg attagataaa tgcntgctct
    15661 ttactgaagg ctctttacta ttgctttatg ataatgtttc atagttggat atcataattt
    15721 aaacaagcaa aaccaaatta agggccagct cattcctccc actcatgatc tatagatcta
    15781 tagatctctc gtgggatcat tgtttttctc ttgattccca ctttgtggtt ctaagtactg
    15841 tggtttccaa atgtgtcagt ttcatagcct gaagaacgag atcagcagcc tctgttccac
    15901 atacacttca ttctcagtat tgttttgcca agttctaatt ccatcagaag ctggtcgagt
    15961 aactttaaat aattggcatt atttaaagtt aggcgcgcca taacttcgta tagcatacat
    16021 tatacgaagt tatgtcgaga tatttaaata ttagacccag ctttcttgta caaagtggtt
    16081 gatatctcta tagtcgcagt aggcggtgtt tctccctctg aaatcataac tcagaaagca
    16141 aaccaaaacg gaacttaaag ttcttttaaa ttacaatgat ttgtttatgt tgtgttgacc
    16201 gtgcatgccg tagcatgtct gtggaggtct caggtcagct tgtagtggtt ggctctcttc
    16261 tttccaccac gcgaggctca gggaccagac tcaggtagtc agcttttcag gtgtggcagc
    16321 aaacaagcat ttatccactg aggcatctgt gtcccatcct tttatccccc acacccaaat
    16381 ttaaatacgc attctcagta tattctttaa aaagctttgt gtcactaagc tttgtttggg
    16441 tgaccttctg ggatgtggac acaggtctat tttagactct taagaaggat gtctcactgt
    16501 tggttacaga ggcatggttt ctctctttat tacagccatc tcttggcgtc aagaagcctt
    16561 ccaaagccct actctttgtg atcctgagcc ccgtgggacc ttatttttct agtggatctt
    16621 ttactccggt gtccctgtgg gccaatccaa agtacatcag agcctggaaa ggtgggactg
    16681 gagactgcaa gatgggcggg taagtcggtc cctgtgtcct tcctgtatag tgacagttgt
    16741 gcgctttcaa tggcggtgtg cttggtagag agatggcgca acgcaattaa tgataacttc
    16801 gtatagcata cattatacga agttatggtc tgagctcgcc atcagttcaa agatttagct
    16861 ttgtaaacgt gttagttgtt ggaggtgttg cttgtggccc ctctaaggca gcagtgtggc
    16921 atgggtattg tgtcccactc gaccggcaag aaagacgcaa caaactggaa tcttccgtgg
    16981 caaaagcttt attgcttact tcttcaggaa gaccttgaac cgggaaaatg gcgccgcctt
    17041 tataacccgc agtgtgacgt ttcagcaccc cgatatggcg tgacggctcc tgattcgttg
    17101 ctcgcccatc accccactat tacactccga gaatgggagt gactaggcgt gagttcactc
    17161 ttgcacctgc gtacaaggct tgtttactag ttaggcacag cggaggccag cgccatctta
    17221 taatggcgat tgctcgcggc acggctctcc acacatgggc atggagcagt gacttgaacc
    17281 agggtccttt ccagctagag actggccctg gggatgatgc tttcacttgg ctccagggaa
    17341 ccctgtctgg aaaggtgact atttcactgt ctgcttagcc acagatggca ggctcacctg
    17401 ctgtgaaggg gcagaggaaa gccctgcttt ggctctgcct ggccagcctc accggccatc
    17461 tgtagctggc aggaaacaaa agagggtttg agatgtgctg gccagtttca gcgttgactt
    17521 ggctctgaat tcagaatgtc cctcggtgga tacaacagaa ggagcaagta tcacggactc
    17581 gtccctggtc tgaatcaact ggtgacatac agagatgctc ctggaaagtg actttcagtg
    17641 atgttctgag aggtttagga aagtcattgc ccagggatag gcctctgata tgagggctga
    17701 tggcagcaag atgcgaaagg tatcccacaa gccccatgtg ctccccaccc cctacagagc
    17761 atttatagat gaccagcttc tctagaaaac tgtagcttgg gcgcctcagg tcagcttcct
    17821 ctgtctttgt gtatgcaaca ctgttgcgtc tcacctgagc ggtatttgct acagaattaa
    17881 ttctagcctc ccagcttagc agtcaccagc acttgctgct cttctagaga tcctaagtta
    17941 agctctctgc ccccgtgtca gcaggctggc aacaacctgt aactcctgct ccctgggatc
    18001 tgaggctctc tcatggcctt tataggcaca tgcacatgac acacacacac acacacacac
    18061 acacacacac acacacacac acacggagag tgcatatttg tgttactgtt catgttgcca
    18121 gtgagcaagg cagggactga gtgagcaagg cagggactga gtgagcttta ggcctgcctt
    18181 attcacagag cccgtgatct aagaagatag tagaataaca acttaatatt atctcacctc
    18241 acaccttgtc tgcatccagc aagcttcaca gggaggggtg gagttaacac aagccagcct
    18301 tcaaagcaag tgctctgctt gcatgcaagt ctgcgaacca tgctctttcc tggtaccctt
    18361 ggaggccaga cgagtacttt taggtagtca ggggttgcca tgtgagtgct aggaactgaa
    18421 ctggggtcct ttgcaagaga atcaagtgct cttaagcacg gagatgtctc tccagcttct
    18481 gcacctctct tgttattgtt tcttgccatc tctgactgca ttctctaata tttggaatta
    18541 aaccctgtga agtgtgaatg gtgtagacca gggagatggc agggaggtta acccagaaca
    18601 aactctgagc agtgtgcatg cgtgaagctg ttaatatgta tgcttggact cctaatgtgg
    18661 agtataaatg tctcctcatg ggagagaata ttgaagtgcc cctcttcagt cctccagccc
    18721 ctccattatt tgtatagtcc ttcacgtctt gtccccttct ctgcccatgc caacaaccag
    18781 gtttcatggc acctgtatca gtttcaaagt tggtcactct ttcccattgc aagttgctcc
    18841 caaaccttcc tgtggggaaa tgtggtctcc ctcagtccaa ggctgtccct gggctatcat
    18901 ggtgacatcg cagttacatg aagacattaa tgccacctgc agggtgtctt gacataagtg
    18961 acttgacaca gtcacaggtt ctgagcctaa gggtgtggac atcactgagg gagagaatat
    19021 tctgcaatgg ctttaaggtt cccaaaaggt gcttggaagc taaaagtgtg actgcatcgc
    19081 ctgaccattt cctgcgctga cgtcatgtgt gggcgtggtc tccagtcatg tgtgggcgtg
    19141 gtctccagtc atgtgtgggc atggtctcca gtcatgggaa atagaaaacc aggtcacttt
    19201 ggactcgcat ttgaagccac taaacagcga gcgagcgagc gagcgagcga gcgagcgagt
    19261 gagcgagaga gagagagaga gagagagaga gagagagaga gagagagaac gagagagaac
    19321 aaagaaaaca aaatgtagag tatttaacta aataagcccg aggttggcag acttctcagt
    19381 gaggctttgc ctttcccaac gattagaggc caagcatgct gagagtgggt agagatgtat
    19441 tagcctgagt agttagttga tcactaggct cattccttgt agcattctga gagtgagtgg
    19501 agatgtgtta gcctgagtag ttgaccactt ggctcatacc tggcctgagt ttcaggttag
    19561 ttctcaaatc ctgtttcccc tcctccctct gccagggcag catttgtctt ttaacatcca
    19621 tgtttgtctg tcctacaggt acttaagtca tttgtgtgtc gcgtgttaat gtatgtgcca
    19681 gggcatgcat ggtgggtcag aagacacctc atgggactca gttctctcct cccactgcgt
    19741 gggccctggg atcaagccca ggtcactagt cttggcagta ggctccttca ctgactgagc
    19801 tcttttccgg cccagggaag aagttttttt tttaaaggga agtgttttgg aggagatcag
    19861 tcagtttgtt ggagcctcaa cccagggtat agcctatggt tttcccaggc aagacaatgt
    19921 caagtattcc ttagccaaat tataagacca ctaattttcc aaatttataa gtggattata
    19981 gtaatcttgg gggttggcag agaaagagaa agactcaggt agacatgcgc ctcctgtccc
    20041 agtgtctgga acaattggtc ctcccttccc cccgttggtg tgtccacagt gcatgtatgg
    20101 agatcagagg ataaatcgtg gaagtgggtt aactcttgcc accctgtggg atctgaagat
    20161 gtaaggtcag taggccttcg aaggaagtga gccatcttgg ccaccttcaa gtcttaaacg
    20221 ctaactctct ctctctctct ctctctctct ctctctctct ctctctgtct ctctctctgt
    20281 ctctctcttc ctctctcttc ctctctcttc ctctctctct ctctctctgt ctctctctct
    20341 ctccccctag caattatgga gcctcccttc tggcacagtg cgaggccgtg gagaatggct
    20401 gtcagcaggt cctgtggctg tacggcaagg acaaccagat aactgaagta ggcacaatga
    20461 atcttttcct ctactggata aacgaagacg gaggtaattg ctcttctggt gtcttgacag
    20521 ctgatgctgt caagagcgca gggggcgggg acatcgaggg cagtgcgggg ctggggacta
    20581 tagtggctgc tgtggagacc gcatcagggt tggtttttat tcttgtttct gctgtttggc
    20641 ttgaaaagct tgagactgat ttcaaactga gctgctgtac atatggctca gcatgtaaaa
    20701 gccctggctg ctcttgcaga ggacctgagt tctgttctga gcaaccacac tgtggctcac
    20761 aactgtctgt gactctaatt tcaggggatc cagccctctt ctggactcca ggggtactgc
    20821 acccatgcac gcacacacag tgtatagccc aggataacct tgaagtcatt ataccacaag
    20881 ggctggcttt gaaatgttag atcctccatt ctgtatggca agaatatatg ggattgtatc
    20941 atacctggct tgagtgtttt gtt
//