LOCUS       KO-first_condition_ready_125028:124995_MGI:101878        20677 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Ccl8 targeting project(s): 125028:124995
ACCESSION   unknown
SOURCE      Mus musculus.
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 115800
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            9488..9615
                     /db_xref="ENSEMBL:ENSMUSE00000401172"
                     /note="ENSMUSE00000401172"
     misc_feature    4275..9891
                     /note="5 arm"
     misc_feature    9919..9943
                     /note="B1 Gateway"
     misc_feature    9956..10003
                     /note="Frt"
     misc_feature    10004..10503
                     /note="mouse En2 intron"
     primer          complement(10072..10093)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9968..9991)
                     /note="FRT3"
     primer          14169..14187
                     /note="seq primer NF"
     misc_feature    14229..14236
                     /note="RE sites"
     misc_feature    16029..16062
                     /note="loxP"
     misc_feature    complement(14237..14268)
                     /note="Rox"
     misc_feature    complement(15989..16020)
                     /note="Rox"
     misc_feature    14269..14290
                     /note="polylinker"
     CDS             14849..15445
                     /note="puromycin N-acetyl transferase"
     misc_feature    14964..14969
                     /note="Mutated RsrII site"
     intron          10504..10878
                     /note="mouse En2 intron"
     misc_feature    10876..10878
                     /note="Splice Acceptor"
     exon            10879..10932
                     /note="mouse En2 exon"
     primer          10779..10800
                     /note="seq primer FCHK2"
     misc_feature    10933..11055
                     /note="1Dleader"
     misc_feature    11056..11107
                     /note="F2A"
     misc_feature    11203..11919
                     /note="EGFP"
     misc_feature    11113..11202
                     /note="3X NLS"
     misc_feature    13985..14223
                     /note="SV40 polyadenylation site"
     misc_feature    13018..13962
                     /note="ERT2"
     promoter        14301..14812
                     /note="PGK promoter"
     misc_feature    15556..15988
                     /note="PGK pA"
     primer          14043..14066
                     /note="LRPCR RAF5"
     misc_feature    11929..11982
                     /note="T2A"
     misc_feature    11979..11979
                     /note="A to G to kill RsRII site"
     misc_feature    11983..13008
                     /note="Cre"
     misc_feature    10894..10933
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    10894..10909
                     /note="FCHK QC prefix region"
     misc_feature    10910..10927
                     /note="FCHK QC critical region"
     misc_feature    10928..10933
                     /note="FCHK QC suffix region"
     misc_feature    16085..16105
                     /note="B2 Gateway"
     misc_feature    9892..16135
                     /note="Synthetic Cassette"
     exon            16486..16597
                     /db_xref="ENSEMBL:ENSMUSE00000108215"
                     /type="targeted"
                     /note="target exon 1 ENSMUSE00000108215"
     misc_feature    16136..16715
                     /note="Critical Region"
     misc_feature    16739..16772
                     /note="Downstream LoxP"
     misc_feature    16716..16795
                     /note="synthetic loxP region"
     exon            17052..17322
                     /db_xref="ENSEMBL:ENSMUSE00000108216"
                     /note="ENSMUSE00000108216"
     misc_feature    16796..20677
                     /note="3 arm"
BASE COUNT     5547 a   4971 c   4863 g   5295 t     1 others
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggactttct tgtttgtccc caatagttca ttttaatact gtcatataaa
     4321 accagatata actatacagg tctcatattc tttttaaaaa tcccaacaat gtagttttaa
     4381 acgtaaaaaa ctgcaggtta atttagaatt gaaacatggc tatctttttt cttgctccaa
     4441 gaaggaagaa tctttgcaca gctacaacca aatcaatgta aaccaaaaat tcaatagtgt
     4501 aaatagctca gtgtctgaca gtaggggctc actcatgatc ttttgagctc caaaggaggt
     4561 gcacctctcc agctctgcca tctgcagcat acacagaatg tctggtaagc tcagcttagc
     4621 tccgttccac agctgctgct acccttagtg gaggttccat gatcttggaa tctctaatat
     4681 ttgggggtat aattaatata attaatcata agactgtctc aaactgaggc tgcacctttc
     4741 caagttgttt ctcttctgtc ctcttcaaag tcaacaaact tgacacatgg tactaagccc
     4801 caggttttgt ccacagctcc ttcacgtctg gggactccag gacactccaa ccctgccaca
     4861 cagtgccaag cctcagctcc tttctgtggc tcaagctatt atgccccaac tttaacccct
     4921 tggatggatg ttgatgaatt ctgtctggtt ttggacattc cttgatatag ttcttggtcc
     4981 aatggtttag tataaattgg ctattaaaat tctatttgtt tggatgtcct ttcgtatttc
     5041 aacacattct gttcatgttt ttttttccag accattgagt ttggtcgttc gttagcactc
     5101 aattttcctc attgccattc ttctgccagt acaaaaacaa aacccacaac actcacctca
     5161 aagggaagaa ggaattattc tggaactaaa tgtgagtgac cgtggcccca aaatacaaat
     5221 ttatgttatc tcaaagtccg ttgtccaagg tgatgacaga ttgatgaagt ttttacaagc
     5281 agcagaacca agaaactcac aaataaatac acttttaaaa tacattaaat aaacagttat
     5341 agcaaagtag aacaaactgt ctgctatggg cttcagatgc tgtctgttgc tattcatagc
     5401 ttttggttgg tggaaattgg acttctggta agttagcact ttccaaaaag tttcaatctg
     5461 tcagtcatgg gatgttaggt cctgcccaga tgtgggcaaa caatggtgat ataggatgct
     5521 aaagatagtt caaggtaaat tggaattaag ctctagactt gagagcattc tgacctctct
     5581 acagtcatca gatctaacca gttaacgagc tttacagaaa gtttagttga tggtgcaata
     5641 ccaaagctaa gaaaagaggc attgcttaaa agctaccagt tagcattcta gatagtctta
     5701 tgattaatta tattaactca aacttgaagg cagtgataat tttttaaaat cctcttttct
     5761 ccctctctct ctctctctct ctctctctct ctctctctct ctctctctct ctctctctct
     5821 gtctcatgct agactaagtc ctgggcaata gaacaaatga tcatggttag gcatttttaa
     5881 aattttgctt tatttttgtt tatggggcaa agccaatctg tcccctagaa tctctaaaaa
     5941 taatggaatc ataaagtctg tagtcaattt aaaagatcat tgattttgga atgcctatga
     6001 gcttgtgtgc tcacatatat gtatgccata ggatccacct gatggagtgc atcccaaatc
     6061 caaccgagag cgaactggtg caattcatgc cgctattgta ccaatggaca tgtcttgcca
     6121 ggacagcatt gttcacagct agcatccaca tatgaaggag aacataatgt ttaggtctgg
     6181 gttaccggga ttgcttctaa ctccatctat ttgcctatta atttcacgat tttgtttttc
     6241 tttttttaaa ttttattaga tattttcttc atttgcattt caaatgctat accaaaagtt
     6301 ccctatacct cccctccccc caccctgctc ccctacccac tcactcccac ttcttggccc
     6361 tggcgttccc ctatactggg gcatataaag tttgcaaaac caaggggcct cccttctcaa
     6421 tgatggctga ctaggccatc tgctacatat gcaactagag acatgagttc tgggggtact
     6481 gattagttca tattgttgtt ccacctacag ggttgcagac cccttcagct ccttgggtac
     6541 tttctctagc tcctccattg gtggtcctgt gttccatcca atagatgact atgagcatcc
     6601 acttctgtat ttgtcagaca ctggcatagc ctcacaagag acagctatat cagagtcctt
     6661 tcagcaaaat cttgctggca tatgcaatag tgtctgtgtt tggtgactga ttatggaatg
     6721 gacccctggg tggggcagtc tctaaatggt ccatccttga tgtggagaaa gatgaacaca
     6781 cctccattgc tgatgggatt gcaagcttgt acaaccactc tggaaatcag tctggccgtt
     6841 tctcagaaaa ctagacatag tactaccaga agatccagca atacctctcc tgggcatata
     6901 cccagaagat gttccaactg gtaataagga cacatgctcc actatgctca tagcagcctt
     6961 atttataata gccaaagctg caaagaaccc agatgtcctt taacagagga atggatacag
     7021 aaaatatggt acatttacac aatggagtac tattcagcta ttaaaaacaa tgaatttatg
     7081 aaattcttag gcaaatggat gtatctggag gatatcatcc tgtgtgaggt aacccaatca
     7141 caaaagaagt cacttgatat gcactcacta ataagtgtat attagcccag gaacctagaa
     7201 tacccaagat acaatttgca aaacacaaga aaatcaagaa gaaggaagac caaaacatgt
     7261 atacttcatt cctccctaga atagagaata aaatacccat ggaaggagtt acagagacaa
     7321 agtttggagc taagaagatt ttgtttttct taacagttga gtaatagtcc actatgcaaa
     7381 tgtgccacat cttcattggg ttgtttttca atttctgaat attgtgaata gagcaccatt
     7441 gaacatatat atgcaagtgt ctccatagta ggatacagag tcctttgggt ggatgtctaa
     7501 aagtggtata gatcagttcc taggatttgg aagaaccctc cacactgatt tccatagtaa
     7561 aaaattctgt agtaggtggc agagtatgtg tgcagacttc tatttgggtc ctcattttta
     7621 tacatcaatc agtgtgtctg gttttgtgct ctaactatta agattataaa ctcttagatt
     7681 catttaatac taaaggtgat aaacactttg gttcaaacat ttcacctcaa acttgtacca
     7741 actcctgctg cctttgtggc ctgcagaaat gctgactcgt gttcttcttc acttacgacg
     7801 acagttgggt attgtaagtg cctaagcttg agtccaacaa cattcccaat tctccgtttc
     7861 aatgtttctt aaatcatttc ttttcatgtt tagatatttt gaaaactgtc ttaggcattt
     7921 gaaattctct ttaatatttt ctaagtgaca aacagtgtga taaatcatgt ctgcagggaa
     7981 ggttcaaatt agtaagccat gcccttagtc catcatgctt tcttatgtct gtttgccttc
     8041 tcacacttca aaccttgtct gaaacaagtc ttcatttcgg aaaaagactc tgtagacaca
     8101 gggaaaataa tcacttggat taaggagtgt gatgactcat gacatactga ggagtttggc
     8161 cttgcttgct ttggtttctt tgggttgttt ttgttgttgt tggggttttt tgtgtttgtt
     8221 tgtttgtttg tttgtttgtt tgttttaggt catggtgtct tcttgcttgt tataacttta
     8281 gatataatgt actactgtgt gttgcattta aatgttgtag ctgacagagg tcaaaaccca
     8341 gaaaaatcat aaaatattag ccattagctc atggtggctt aatagattgg atataattca
     8401 gagatttaat tctgagtggc tttactaact atatccctca cttgaggatg ttcttcctgc
     8461 ctactagtca ggaatctttg cttacactga cgaggaatga gactgagcat aacactattt
     8521 ggcctccaaa cacattgaac aggatgcttg gccaacagag ttattgtgat tgaagttccc
     8581 agacaaaagt catacataca ttaaggattc tgcaagaaaa cagctctgtg atgtagagag
     8641 ctatgctcct gcaggatgcc acccgctgca ggatccctgc ccctccagaa tccacacgtg
     8701 gtgcccagtt ctccaggact ttcaggctag gccagacata gtagagaaac tctgttatta
     8761 cagcattttt cagactgata tgggagaatt atacaacaag gacggcctgg ggacaaagta
     8821 aatttggaag agccagatct acttaatcct catctagaat actaactaca aaagaagggc
     8881 tgaggtatag ctgccattta gtagtagaat acttacttgg ctgtcatgtg caaggttctg
     8941 gctaacatgt aaaattccta gtaatagaag aaaaaggaag aggagacgga agagaaggag
     9001 acttgagtct atcatgtttt cagggttctt tggagcccat ctgggttttg aaaaacccac
     9061 ctgccttagg gttgcaaaag cctagtgtaa aagggaccag acttaggcct ttctctggtc
     9121 tcctagcacc ctccaaaagg aagcatctaa cctctcttta ctccatggta accttggttt
     9181 ctagtctttc ttctatacag atgtttttca tttctatgtt tcagaatccc tgatccagtg
     9241 ggactgcttc ctccatgctg ctggtttcag atgcttgccc ttcaaagttt ctgtctcata
     9301 gtctaagtta gccaaagatt ttagcatctt attcttgctg agctgccttc cagctgccgg
     9361 gaaatcatct catgatctga tgactattca gcataacaaa gatcttgctt tcatttccca
     9421 ttaggcacaa ctctatctgc agattccctg ctccctataa aaggcagatt agagcctcca
     9481 gactagcaga gttagattga gaggacgcta gccttcactc caaaatcttt gccttcaaca
     9541 tgaagatcta cgcagtgctt ctttgcctgc tgctcatagc tgtccctgtc agcccagaga
     9601 agctgactgg gccaggtaag gtatctcctt tcctaaggga aatggtttag caggcactaa
     9661 catctgagca caaggcatcc cagagctgct gtatgatctg caattttaag atggacagac
     9721 tgagagtatg gaagaagtta gccaaaagcc agtgtgtcaa agctaggcag aaccagcagt
     9781 gagtgtttag agcactgagg ccaggccctg tgatgtctca tgacatcagc cctgggacac
     9841 gatgctgcat gcagccacta ccgatactgc cctctccttg tttgaggaga taaggcgcat
     9901 aacgatacca cgatatcaac aagtttgtac aaaaaagcag gctggcgccg gaaccgaagt
     9961 tcctattccg aagttcctat tctctagaaa gtataggaac ttcgaaccct ttcccacacc
    10021 accctccaca cttgccccaa acactgccaa ctatgtagga ggaaggggtt gggactaaca
    10081 gaagaacccg ttgtggggaa gctgttggga gggtcacttt atgttcttgc ccaaggtcag
    10141 ttgggtggcc tgcttctgat gaggtggtcc caaggtctgg ggtagaaggt gagagggaca
    10201 ggccaccaag gtcagccccc cccccctatc ccataggagc caggtccctc tcctggacag
    10261 gaagactgaa ggggagatgc cagagactca gtgaagcctg gggtacccta ttggagtcct
    10321 tcaaggaaac aaacttggcc tcaccaggcc tcagccttgg ctcctcctgg gaactctact
    10381 gcccttggga tccccttgta gttgtgggtt acataggaag ggggacggga ttccccttga
    10441 ctggctagcc tactcttttc ttcagtcttc tccatctcct ctcacctgtc tctcgaccct
    10501 ttccctagga tagacttgga aaaagataag gggagaaaac aaatgcaaac gaggccagaa
    10561 agattttggc tgggcattcc ttccgctagc ttttattggg atcccctagt ttgtgatagg
    10621 ccttttagct acatctgcca atccatctca ttttcacaca cacacacacc actttccttc
    10681 tggtcagtgg gcacatgtcc agcctcaagt ttatatcacc acccccaatg cccaacactt
    10741 gtatggcctt gggcgggtca tccccccccc cacccccagt atctgcaacc tcaagctagc
    10801 ttgggtgcgt tggttgtgga taagtagcta gactccagca accagtaacc tctgcccttt
    10861 ctcctccatg acaaccaggc tcccaggtcc cgaaaaccaa agaagaagaa cgcagatcgc
    10921 agatcgcaga tctctagagt caccgagttg ctttaccgga tgaagagggc cgaaacatac
    10981 tgtccaaggc ccttgctggc aatccaccca actgaagcca gacacaaaca gaaaattgtg
    11041 gcaccggtga aacagacttt gaattttgac cttctcaagt tggcgggaga cgtcgagtcc
    11101 aaccccggac cggtgcatgt ggaccccaag aaaaagcgga aggtggaccc taaaaagaaa
    11161 cgcaaagtgg atcctaagaa aaaaagaaag gtgcacgtgt ccatggtgtc caagggcgag
    11221 gaactgttca ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac
    11281 aagttcagcg tgtccggcga gggcgaaggc gacgccacat acggaaagct gaccctgaag
    11341 ttcatctgca ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc
    11401 tacggcgtgc agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag
    11461 agcgccatgc ccgagggcta cgtgcaggaa agaaccatct tctttaagga cgacggcaac
    11521 tacaagacca gggccgaagt gaagttcgag ggcgacaccc tcgtgaacag aatcgagctg
    11581 aagggcatcg acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac
    11641 aacagccaca acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc
    11701 aagatccggc acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac
    11761 acccctatcg gcgacggccc tgtgctgctg cctgacaacc actacctgag cacccagagc
    11821 gccctgagca aggaccccaa cgagaagagg gaccacatgg tgctgctgga attcgtgacc
    11881 gccgctggca tcaccctggg catggacgag ctgtacaagc ctgcaggcga gggcagagga
    11941 agtcttctaa catgcggtga cgtggaggag aatcccgggc cgtccaattt actgaccgta
    12001 caccaaaatt tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg
    12061 atggacatgt tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc
    12121 gtttgccggt cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa
    12181 cctgaagatg ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtaaaaact
    12241 atccagcaac atttgggcca gctaaacatg cttcatcgtc ggtctgggct gccacgacca
    12301 agtgacagca atgctgtttc actggttatg cggcggatcc gaaaagaaaa cgttgatgcc
    12361 ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca
    12421 ctcatggaaa atagcgaccg ctgccaggat ataaggaatc tggcatttct ggggattgct
    12481 tataacaccc tgttacgtat agccgaaatt gccaggatca gggttaaaga tatctcacgt
    12541 actgacggtg ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca
    12601 ggtgtagaga aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgtc
    12661 tctggtgtag ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt
    12721 gccgcgccat ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca
    12781 actcatcgat tgatttacgg cgctaaggat gactctggtc agagatacct ggcctggtct
    12841 ggacacagtg cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg
    12901 gagatcatgc aagctggtgg ctggaccaat gtaaatattg tcatgaacta tatccgtaac
    12961 ctggatagtg aaacaggggc aatggtgcgc ctgctggaag atggcgatct cgagccatct
    13021 gctggagaca tgagagctgc caacctttgg ccaagcccgc tcatgatcaa acgctctaag
    13081 aagaacagcc tggccttgtc cctgacggcc gaccagatgg tcagtgcctt gttggatgct
    13141 gagcccccca tactctattc cgagtatgat cctaccagac ccttcagtga agcttcgatg
    13201 atgggcttac tgaccaacct ggcagacagg gagctggttc acatgatcaa ctgggcgaag
    13261 agggtgccag gctttgtgga tttgaccctc catgatcagg tccaccttct agaatgtgcc
    13321 tggctagaga tcctgatgat tggtctcgtc tggcgctcca tggagcaccc agtgaagcta
    13381 ctgtttgctc ctaacttgct cttggacagg aaccagggaa aatgtgtaga gggcatggtg
    13441 gagatcttcg acatgctgct ggctacatca tctcggttcc gcatgatgaa tctgcaggga
    13501 gaggagtttg tgtgcctcaa atctattatt ttgcttaatt ctggagtgta cacatttctg
    13561 tccagcaccc tgaagtctct ggaagagaag gaccatatcc accgagtcct ggacaagatc
    13621 acagacactt tgatccacct gatggccaag gcaggcctga ccctgcagca gcagcaccag
    13681 cggctggccc agctcctcct catcctctcc cacatcaggc acatgagtaa caaaggcatg
    13741 gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc tctatgacct gctgctggag
    13801 gcggcggacg cccaccgcct acatgcgccc actagccgtg gaggggcatc cgtggaggag
    13861 acggaccaaa gccacttggc cactgcgggc tctacttcat cgcattcctt gcaaaagtat
    13921 tacatcacgg gggaggcaga gggtttccct gccacagctt gatgaagatc tgagctctta
    13981 attaatgatc ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct
    14041 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
    14101 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    14161 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    14221 ctggatccgt ttaaactaac tttaaataat tggcattatt taaagttaca tatggcggcc
    14281 gccctaaggc tcgacctcga aattctaccg ggtaggggag gcgcttttcc caaggcagtc
    14341 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
    14401 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
    14461 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
    14521 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
    14581 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
    14641 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
    14701 caggggcggg gcgggcgccc gaaggtcttc cggaggcccg gcattctgca cgcttcaaaa
    14761 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcatcca
    14821 tctagatctc gagcagctga agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
    14881 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
    14941 cgccacgcgc cacaccgtcg atccagatcg ccacatcgag cgggtcaccg agctgcaaga
    15001 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
    15061 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
    15121 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
    15181 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
    15241 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
    15301 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
    15361 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
    15421 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
    15481 aaggagcgca cgaccccatg catcgatgat atcagatccc cgggatgcag aaattgatga
    15541 tctattaaac aataaagatg tccactaaaa tggaagtttt tcctgtcata ctttgttaag
    15601 aagggtgaga acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg
    15661 tggggtggga ttagataaat gcntgctctt tactgaaggc tctttactat tgctttatga
    15721 taatgtttca tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc
    15781 attcctccca ctcatgatct atagatctat agatctctcg tgggatcatt gtttttctct
    15841 tgattcccac tttgtggttc taagtactgt ggtttccaaa tgtgtcagtt tcatagcctg
    15901 aagaacgaga tcagcagcct ctgttccaca tacacttcat tctcagtatt gttttgccaa
    15961 gttctaattc catcagaagc tggtcgagta actttaaata attggcatta tttaaagtta
    16021 ggcgcgccat aacttcgtat agcatacatt atacgaagtt atgtcgagat atttaaatat
    16081 tagacccagc tttcttgtac aaagtggttg atatctctat agtcgcagta ggcggttcca
    16141 gaaccatatg tggatatttg tacactgagg ggcttcctag attaaaaaat aaaataggtt
    16201 gattctaagc ccacagcagc aaggtgatgg acagcgagtt cttgggactt actttcctgt
    16261 gtcagaagta tgggatcagt caaagagatc tcatgataaa cagtcatcag tgaggaatcc
    16321 tgtggttcaa cagaggacac tgactcctaa gacatattct gtcactagac cctagataca
    16381 aaacttggga acagtgactc ctaggtgcag cccaaccctc atcttccctt atgagaggac
    16441 tctgagatgt cccctaaccc tctttcttta caaaatggct tccagataag gctccagtca
    16501 cctgctgctt tcatgtacta aagctgaaga tcccccttcg ggtgctgaaa agctacgaga
    16561 gaatcaacaa tatccagtgc cccatggaag ctgtggtgta agtgattcct gcccagaaaa
    16621 cccagtcaga gtcaagggag agtggtcagg acacacagat gagtgagtca aacaaataca
    16681 gcctcttatc tggaccacgc cttacccaca taggggagat ggcgcaacgc aattaatgat
    16741 aacttcgtat agcatacatt atacgaagtt atggtctgag ctcgccatca gttcatgact
    16801 ggaagagaag aaatctgtaa ttttccagga gctcagaggg attttcccca gttcctttcc
    16861 acaatagatg agaaagcttc aagtagggca gcaagatcag ggcttcctcc tcagggttct
    16921 cccttccacc ccttcctcat cctcccctcc tgccaagcag ctccttcacc tctggagcaa
    16981 gagtgaatgg ggtctgtcga gctgtgggcc atgctctgag cactatttct gaaccttcca
    17041 tctctccaca gtttccagac caagcagggt atgtctctct gtgtagaccc cacacagaag
    17101 tgggtcagtg agtacatgga gatccttgac cagaagtctc aaattctgca gccttgaacc
    17161 ttcacacctg agttaagaga cagccaaagc tggaagttct cccctaatct tctccaggca
    17221 gagagatgtt acaagcagat ggtgcctggg ctgcgtgttt tctcatcctt gtctgttata
    17281 tgaacaactg aaataaaagc ttacactgat ttgcaaatgt aacatgggca tttttattca
    17341 aagtgaagtg atgggaagtt atgatcaatc tcagagagca gcaggccaaa tgaggggagg
    17401 tgttcaaggg ttcctgtgta ttatttgggt ttcattttat ggggttcaag tgaaggaaga
    17461 gccttgttac tagagatgga gtgggtggtt ctctactggt tgagaggctt caattacatt
    17521 aaaccattgt tcattgctca tatctgttcc cataacgcat cggcttttta gcataggcat
    17581 gcccagttgt gcaaaggcat gaatagtaaa taggggattc tttctgaaag ttcacttgag
    17641 gttatactcc acctcattgt acatgtacca caagctggtc tcagtcagcc ccagactctg
    17701 ttccagactg tgtttgacta gtgacaaagc ttatcaaggc tttactaaat ggagcaattt
    17761 atttcatggt ttaaaccctt ttgaagttga gaatgctagg ttgctaacag gttgcttgct
    17821 atactctcca aagatgaggt gtgtacatag tccaaaccaa gtagaatcct gagttgcata
    17881 gatattccct atgcttgcct tcctcacctt ccccctgaaa aactttgttt tcctgattca
    17941 tagagagcta taccctctgt gtctggcaca tttacatgta acacacaaca ggaacattgt
    18001 aagtattaat attagtcaca gttctctaga gcaccagagc caaaagaatg aatatgaatg
    18061 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgcattca cctgttctca
    18121 gaaatgttct atacctattt ttccgtgttt ctgtgaccta ctttccttaa ctgcctggcc
    18181 tcacttctgt agccatgctt acactattgt cttgcactgt gttgacaagt agtctttcag
    18241 tggccgtctc ccaccaggaa gatcaagaaa tcagtagttg ttcatccata tgccttggca
    18301 gttctaatct gaagccaaag cctggaagat ttctgaagaa ccagtgggct tccttgcatg
    18361 gtggaagccc aaagaagctc attctaatat cagtaaagga attggcagca gcaatgtaga
    18421 tgaacttgcc aatgagaatg aaagccaagt aagaaaaaaa attttacctt cttccatatc
    18481 tctttatggg atgacattgt gtcagaacta acagcacagt atctaaagtt aaaataatca
    18541 agacacatca caacctaaag aagtaaagct aacagaagaa ggaaatcagc atcttaatca
    18601 accaacactc cgatggatgt gtcactgtct tacttttgtt ctcccagatc ttgacacaac
    18661 tgacatcagg ttaaaagccc catccagggc ttgaaaccca gaaagtaagc tgcaactcca
    18721 tacaaaacag ggatgaagac ctgggtcacc tgctaaatca ggaataaaag ctaagtccat
    18781 gggttcccag aaagtctctg agtaaaagtc tctgaatgta ctaacctggc ttttgttttg
    18841 gcttctgtag ttctgcttct tactaactga aattttccaa ccataatgtg ggttctcagt
    18901 ataaaagaca aagacctgtg aggtcctcgg cttcatggtt tgggcaagtt ccctgtgtag
    18961 tcagttgtgg accagcaata aagacttttt tctcggcttt tatgatcgtc agtgtgattg
    19021 tctcaggtag gcttacctca cagcagtttt atctcatgtt gacacaagct aaagtcatta
    19081 gcaaagaggg agcctcagct gagattggcc tgtgggaaaa tctgtggggc atttgattga
    19141 tggttgatgt gggagggctc agctcactgt agatgagacc acccccatgg atgggtgatc
    19201 ctataagaaa aggggctgag caagccaagg agagggagac tataagcagc accaaggtct
    19261 ttgcatcagc tcccacctgc acttcttcat agtctctgca tcagctcata cctccaggtt
    19321 cctgccctga gttcccagtg atggactgtt acttgaaaat gtaagatagc tgggcagtgg
    19381 ttgcatacac ctttaatccc agcacttggt aggcagaggc aggcagattg ctgagttcaa
    19441 ggccagcctg gtctacaaag tgagttccag gacagccagg ggtacacaga gaaaccctgt
    19501 ctcaggaaaa agaaaagaaa agaaaaaaga gaagagaaga gaagagaaga gaagagaaga
    19561 gaagagaaga gaagagaaga gaagagaaga gaagagaaga gaagagaaga gaagagaggg
    19621 aagggaaggg aagggaaggg aagggaaggg aagggaaggg aagggaaggg aagggaaaga
    19681 aaaagaaaaa gaaaaagaaa aagaaaaaga aaaagaaaaa gaaaaaagag gaaagaaaaa
    19741 gaaagagaag agaaagaaag gaaagagaag ggaaaaagaa aaggaaaaga aaagggaaaa
    19801 aaggaaaaga aaaggaaaag gaaagaaaaa gaaaaagaaa aagaaaaaga aaagaaacgt
    19861 aagatagaac aaacactttg ctttcaaacc cctaaagtga gacagtaatc aattgcttta
    19921 atcactatgc ttttcccctg agtcacagaa tctttcctga cataaagact tggttcagga
    19981 aggttggaag catgagtagg caaacagaca tagtaaaagt ttgatgaatt aagaacagag
    20041 tcttcattcg gacctttact aaagctgtga ctggcagtgt gtttgtcatt tacatagtat
    20101 taaggaaaat tccaaatgcc ttggacaact ttcaaagcat ctaaacataa aaaaaaaatt
    20161 aagaaataga aaggatgaga attagaaatt ttgtcagcag agtttgcaac ttagttactt
    20221 gggtatccaa ctgtcattgt acttgaaaat aaatcccacc tagattttcc aaagtttaga
    20281 agggtgtgtc tccagcagaa gttggtacaa atttgaggtg agaaatataa agctaactgg
    20341 gttttctcct tagaagtgat ggaacagaaa atgagtagac ataccccata aagtcctttc
    20401 tctccagatg ggagctcttg atgcatgtct tttcccaatt gatgaaatca cctgatttcc
    20461 cccttttatt cccctagctt tccctgttct gggagctcag ataggagccc tgtaaccgat
    20521 tttgattttt gcttgcaaca atatatgttt ttcttttata gacatgcctt agacacgttt
    20581 tactcaatgt ttataggatg cttagcattt tttcagttag atagatgttt gcccaaaaga
    20641 gtagataata gaattaaaag tactgatagg gactttc
//