LOCUS       278188#pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21846 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 278188
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000022616
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            6919..7039
                     /db_xref="ENSEMBL:ENSMUSE00001206274"
                     /rank="2"
                     /note="ENSMUSE00001206274"
     exon            8512..8660
                     /db_xref="ENSEMBL:ENSMUSE00001233196"
                     /rank="3"
                     /note="ENSMUSE00001233196"
     misc_feature    4275..9580
                     /note="5 arm"
     misc_feature    9608..9632
                     /note="B1 Gateway"
     misc_feature    9645..9692
                     /note="Frt"
     misc_feature    9693..10192
                     /note="mouse En2 intron"
     primer          complement(9761..9782)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9657..9680)
                     /note="FRT3"
     primer          13844..13862
                     /note="seq primer NF"
     misc_feature    13904..13911
                     /note="RE sites"
     misc_feature    15704..15737
                     /note="loxP"
     misc_feature    complement(13912..13943)
                     /note="Rox"
     misc_feature    complement(15664..15695)
                     /note="Rox"
     misc_feature    13944..13965
                     /note="polylinker"
     CDS             14524..15120
                     /note="puromycin N-acetyl transferase"
     misc_feature    14639..14644
                     /note="Mutated RsrII site"
     intron          10193..10567
                     /note="mouse En2 intron"
     misc_feature    10565..10567
                     /note="Splice Acceptor"
     exon            10568..10607
                     /note="mouse En2 exon"
     primer          10468..10489
                     /note="seq primer FCHK2"
     misc_feature    10608..10730
                     /note="1Dleader"
     misc_feature    10731..10782
                     /note="F2A"
     misc_feature    10878..11594
                     /note="EGFP"
     misc_feature    10788..10877
                     /note="3X NLS"
     misc_feature    13660..13898
                     /note="SV40 polyadenylation site"
     misc_feature    12693..13637
                     /note="ERT2"
     promoter        13976..14487
                     /note="PGK promoter"
     misc_feature    15231..15663
                     /note="PGK pA"
     primer          13718..13741
                     /note="LRPCR RAF5"
     misc_feature    11604..11657
                     /note="T2A"
     misc_feature    11654..11654
                     /note=" A to G to kill RsRII site"
     misc_feature    11658..12683
                     /note="Cre"
     misc_feature    10583..10608
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    10583..10598
                     /note="FCHK QC prefix region"
     misc_feature    10599..10602
                     /note="FCHK QC critical region"
     misc_feature    10603..10608
                     /note="FCHK QC suffix region"
     misc_feature    15760..15780
                     /note="B2 Gateway"
     misc_feature    9581..15810
                     /note="Synthetic Cassette"
     exon            16174..16344
                     /db_xref="ENSEMBL:ENSMUSE00001272117"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00001272117"
     exon            16912..17323
                     /db_xref="ENSEMBL:ENSMUSE00000497161"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 2 ENSMUSE00000497161"
     misc_feature    15811..17538
                     /note="Critical Region"
     misc_feature    17562..17595
                     /note="Downstream LoxP"
     misc_feature    17539..17618
                     /note="synthetic loxP region"
     exon            18261..18365
                     /db_xref="ENSEMBL:ENSMUSE00000123109"
                     /rank="6"
                     /note="ENSMUSE00000123109"
     exon            21006..21235
                     /db_xref="ENSEMBL:ENSMUSE00000123110"
                     /rank="7"
                     /note="ENSMUSE00000123110"
     misc_feature    17619..21846
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggacctttc agttcctggg acagcgcata ataaataaga tgttgtgtat
     4321 ttaaagacgt gctgcagaca tccagacccg ttttgctgag ctgactgcat tcatccagtt
     4381 ctttagagaa atgctccacc aggccgagac gtctgatccc accggatcac tttatagatg
     4441 gtaatctgag gcctggagac cattagaggc tcagcctcca aacgttatat gaataagaaa
     4501 ctgaagccct ttggcttttc cagaatttct gggcccagtt ttaaaaatgg tgctggtgct
     4561 gtttttaaag gaagaagtta ggaattcaag agtctataca agttctctta acaggatcat
     4621 acaatttagt gcccgaccct gtgagaatag taaaacaaac tgcaaatggt agtcaatcaa
     4681 tggaatatta ggcagcagca aatgtgtgac ttttaaaaat atttaaaaac aggacctatg
     4741 aagggatggt ttataaaaat gcattggaaa aaaatggaga aaatacgcca caatgggaga
     4801 ggctatgttt tcctactaga taaggaggtc cttggccctg gtggggctga tgcacccagg
     4861 gtagttggca gccaggcagt aggccagcct ccaaaagaag acaggaagta agtcagtgta
     4921 gagggaaggc agcgggctga actgagcagc gttgtccttg gaacaacaac accacagcgc
     4981 cgggaaactt gggagactag cagattctga cccggagtcc atattaccag cagacctaga
     5041 gggagtctgt tcttagcttt actgaataga ggatcgcttc tttgaggtct gtatcaggtc
     5101 cattttacag ataaagaaac caaggccgag agatttagga ctttttattg tcaaggaaag
     5161 aatattaaaa tgaggtttca gagggctgca ttcttcctgg ggggtggaga gtgggggtgg
     5221 gggttggcta ctggcttgca gtgtgtcttg gggagcctgg caggtgtctg ggcgttggca
     5281 ttctctagaa gggcccaggc tgaagagcaa gcaaccctcc ctttcttatt tgggaacttg
     5341 gtggctctcc agaccccagg tctcctttgg caagccccaa gctccctgct ccctgtagct
     5401 catcttgata ctgcttcgcc tcccattcaa tggtcttcct tggctgccct gccctgctgc
     5461 ctcttcctgc ctcggatcag aagccatttc caaggctgtt aaatggcttt tcccaaaatg
     5521 gtcatgcttg aggctactcc cacctacaga tatttctgtg gctttgtctc tgatgtgtca
     5581 aggggtctgt ccctgcctgg agcatttcag gcctcagtga gcacctggga ggtctatgag
     5641 atcagggcct tcctagtgga tcatgaacca ggctggctga gccacctacc tatccctggg
     5701 aaagtctctt ctcccacacc actcctcctc tgctctgcgc gcgtaattaa gccctcagca
     5761 gaggccttgc ctattaatga tctgcccagt gtgttgatgg gcctgcctag ctgagagcag
     5821 gttttaagag tgagccattc caggaagaga gatcgctccc ttttccctgt tcctgtgggt
     5881 ataggaagtt ggtgagccca gataagctat tctgggcagc gttggagatg caaggggtgt
     5941 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgttt atgcaaccaa
     6001 tccagcttct gggaaaggat ccattgtgta atactggggc ttcagcctgt gtcccctggc
     6061 tcagggcagc cggcccagtg gtggcagtgg ccccttggca gagtatgcta gttcaggctt
     6121 agaggcaaca ggcatgtgaa aggaatttgc tggagccaat gggcttgtcc tcctgaaagg
     6181 gctttctggg gcggggggtg ggggtgggga tgggggctca ctgctaatct caggctttgt
     6241 ccttcccctt tctagttctg ccttctctgc agtcctaaat tccttccctt cccattttgc
     6301 ttttacccca ccccctcccc aatccatact cagcctggtg gcacaagcgt tgcccagaga
     6361 tggtgagatg gattgaattt cccgtgctga tggaagggtg tgcatattac cacaggctca
     6421 cttggatagc cttgggaggc tgagggcccc ctaaggggag cccagggctg tgcagtgagt
     6481 ggggatgcag taccatggaa ggggataggc gagggatgtt tgcatgtttc gggagaggta
     6541 tctttagtat ttggcatgtt aaatatgctc tggagacaca ggaaattcat ctgccctgcc
     6601 tcaggctctt atgggaagaa agggactcag cttggagctc ggcccctcca actccagata
     6661 gcctggctcc tctgtgcagc cgggccatgg ttcttcccag ggcctcgctg tctccagtgt
     6721 ctatagaagg ggtcagttta atcttggctt tcttccccag ttcttgagaa gtagattgca
     6781 gtgatgccag atgaggagac agatgagggc cgcgtgttcc aatcccaccc cacctcccct
     6841 ctcagggagc tgtagcaggg tgcagaaaac cctgaagctg cccaggggtt gccatgacac
     6901 atctgctgtt gtttccagga ctccagattc caaggaggcc acgccatgaa gattctcctg
     6961 ctgtgcgtgg cactgctgct gatctgggac aatggcatgg tcctgggaga gcaggaggtc
     7021 tctgacaatg agctccaagg taggtaggct gagactgttc ctctggtgga tgctataggg
     7081 cccagggcag ggccattcct caaagctcag tccccaagtg ctgagggctg ttttcattct
     7141 acatcctggg tctgtccatg ccctctgccc tctctaggtc ctgtcttgat gtttttgtgg
     7201 tcaggtgact tctagacatt gccctctgtc tcaagcccct ctgttggctg cctgtcattc
     7261 ctttgtcatt actggtcccc tcacctggct ctcttactct tctcccaaag ccgtgcccta
     7321 gaccctttga aactttgtca gtcttccctt tgtgtgacat ccagggttct taccgatgtg
     7381 tctccacacc aacccctcct ggaaagccat atccaccaat ttcctcctct ggaaaaaggg
     7441 caatgcaatt tgacatgaag tagtcacctg agagcaccct ctcccttcct ccccatcttc
     7501 caaggctttt ctggaaaccc accttttctc ccttcttctc tcctaacctc ttgctcatag
     7561 ctgaccagtt ctgggggggg gggtcttcgt taaagacaat gtgatcagat tctttgatgt
     7621 ggatacacac ggtgaaatga ttgctgttgt caagctatta gctgtccatc acctcaactt
     7681 atcattgggt tcagacactt aaaatatgtt ctctcagcac acttcaaggg tagaacacat
     7741 tatcaaccac gagctctgct tggaccattg accttcgggc ttacttgtgt tgctacatca
     7801 ttgaagctct tgcccttggc tcagcagtcc cccctcccgc ttctctttgg atgtgtctgg
     7861 caaccgcatc aggtagaaaa cagcagaggt gtgggaaatg gcacttgaca caggataagt
     7921 aagtggtacc accgtgacat gatgtgtcat tgccttcgtc acctggggcc gtgtcatggc
     7981 tatggtctcc atggtgaatg cagctcttca tttatcgtcc cgtctccatg gtgtctggtg
     8041 ctatcctgtc acctgaccct gtgcgttgta gcaaagaagt gccaggattg ggttaacctg
     8101 agcttcacag agataatttc caaaagagca cttttattac accttgatgc ttaggaggtt
     8161 gttttgtttt gttttctgtt ttccttgaaa tattctctgt ggctgttggc tctccagggt
     8221 ggcatggtgg ggtcagtcat agtatggctg cccctctctg caccactcac tcctgagttt
     8281 ctgagctgtg acttggagtc taccatgagc cctttgatgg gacatagagg caggactgag
     8341 gccataagga ctgccacagg cttggggtaa tagtgatggt gtctcagctt gacgccccca
     8401 gcactcccca tcaaagcatg gctttctcac cgtccccctg gcacccaggc tatttctcca
     8461 gccattcctc cccgcccccc tcaactcact gtaccttcct ctctctcaca gaactgtcca
     8521 ctcaagggag taggtatatt aataaggaga ttcagaacgc cgtccaggga gtgaagcaca
     8581 taaaaactct catagaaaaa accaacgcag agcgcaagtc cttgctcaac agtttagagg
     8641 aagccaagaa gaagaaagag gtagggccaa gccttggctg agcccaggct ctccctgctg
     8701 gaggggagga ggggacatgg tgagcaggtg cctttctggg ttgccatggt gtcctaaggc
     8761 ggtcctcgaa gggttccgct aatgctggac tgaggagcca gggaaagcac attcgcttgt
     8821 gggagcctca gtgccgaggc cttgaatttg gtttccctgc aactctggtc cagggcctga
     8881 agcctagatt catttcaata ggtttttcta acccttgctg tgggtagggc atggtgcagg
     8941 actcagcggt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgatatg agcatccaga
     9001 catttcccat tcttttcttc tgtattcaaa atgactttta acatgaaagt tcttattgaa
     9061 atattttaaa atcaatgtaa aattcttgta atctatcctt agacatccat ccattcattc
     9121 attcccactc agatataggg gaggggggca gttttcaaaa cccttagtag aagctgcagt
     9181 tgtggctagc accaggccct gcagagattg tgcttctgta atttataaat tacagacagt
     9241 aaggtatgag cagccacaat aaaatagagc aattataatg ctataccagt tataccatac
     9301 agaaggctac gcagatgtga tttctcccac acaaaatatc tcaattcctc agcttccaca
     9361 atactatttt tagactggtt gaccatggat agctaagaac tgataacaag ggactgccct
     9421 gttaccattt gtttaatttg tcatctacat ttgaacatca atgttgactc tggtacacac
     9481 acacacacac acacacacac acacacacac acacacacac aaacttatcc acagattcat
     9541 ccatccactt aattattttc catattgata tgtagacaca aaggcgcata acgataccac
     9601 gatatcaaca agtttgtaca aaaaagcagg ctggcgccgg aaccgaagtt cctattccga
     9661 agttcctatt ctctagaaag tataggaact tcgaaccctt tcccacacca ccctccacac
     9721 ttgccccaaa cactgccaac tatgtaggag gaaggggttg ggactaacag aagaacccgt
     9781 tgtggggaag ctgttgggag ggtcacttta tgttcttgcc caaggtcagt tgggtggcct
     9841 gcttctgatg aggtggtccc aaggtctggg gtagaaggtg agagggacag gccaccaagg
     9901 tcagcccccc ccccctatcc cataggagcc aggtccctct cctggacagg aagactgaag
     9961 gggagatgcc agagactcag tgaagcctgg ggtaccctat tggagtcctt caaggaaaca
    10021 aacttggcct caccaggcct cagccttggc tcctcctggg aactctactg cccttgggat
    10081 ccccttgtag ttgtgggtta cataggaagg gggacgggat tccccttgac tggctagcct
    10141 actcttttct tcagtcttct ccatctcctc tcacctgtct ctcgaccctt tccctaggat
    10201 agacttggaa aaagataagg ggagaaaaca aatgcaaacg aggccagaaa gattttggct
    10261 gggcattcct tccgctagct tttattggga tcccctagtt tgtgataggc cttttagcta
    10321 catctgccaa tccatctcat tttcacacac acacacacca ctttccttct ggtcagtggg
    10381 cacatgtcca gcctcaagtt tatatcacca cccccaatgc ccaacacttg tatggccttg
    10441 ggcgggtcat cccccccccc acccccagta tctgcaacct caagctagct tgggtgcgtt
    10501 ggttgtggat aagtagctag actccagcaa ccagtaacct ctgccctttc tcctccatga
    10561 caaccaggct cccaggtccc gaaaaccaaa gaagaagaac gcagatctct agagtcaccg
    10621 agttgcttta ccggatgaag agggccgaaa catactgtcc aaggcccttg ctggcaatcc
    10681 acccaactga agccagacac aaacagaaaa ttgtggcacc ggtgaaacag actttgaatt
    10741 ttgaccttct caagttggcg ggagacgtcg agtccaaccc cggaccggtg catgtggacc
    10801 ccaagaaaaa gcggaaggtg gaccctaaaa agaaacgcaa agtggatcct aagaaaaaaa
    10861 gaaaggtgca cgtgtccatg gtgtccaagg gcgaggaact gttcaccggc gtggtgccca
    10921 tcctggtgga actggatggc gacgtgaacg gccacaagtt cagcgtgtcc ggcgagggcg
    10981 aaggcgacgc cacatacgga aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
    11041 ccgtgccttg gcctaccctc gtgaccacac tgacctacgg cgtgcagtgc ttcagcagat
    11101 accccgacca tatgaagcag cacgacttct tcaagagcgc catgcccgag ggctacgtgc
    11161 aggaaagaac catcttcttt aaggacgacg gcaactacaa gaccagggcc gaagtgaagt
    11221 tcgagggcga caccctcgtg aacagaatcg agctgaaggg catcgacttc aaagaggacg
    11281 gcaacatcct gggccacaag ctggagtaca actacaacag ccacaacgtg tacatcatgg
    11341 ccgacaagca gaaaaacggc atcaaagtga acttcaagat ccggcacaac atcgaggacg
    11401 gctccgtgca gctggccgac cactaccagc agaacacccc tatcggcgac ggccctgtgc
    11461 tgctgcctga caaccactac ctgagcaccc agagcgccct gagcaaggac cccaacgaga
    11521 agagggacca catggtgctg ctggaattcg tgaccgccgc tggcatcacc ctgggcatgg
    11581 acgagctgta caagcctgca ggcgagggca gaggaagtct tctaacatgc ggtgacgtgg
    11641 aggagaatcc cgggccgtcc aatttactga ccgtacacca aaatttgcct gcattaccgg
    11701 tcgatgcaac gagtgatgag gttcgcaaga acctgatgga catgttcagg gatcgccagg
    11761 cgttttctga gcatacctgg aaaatgcttc tgtccgtttg ccggtcgtgg gcggcatggt
    11821 gcaagttgaa taaccggaaa tggtttcccg cagaacctga agatgttcgc gattatcttc
    11881 tatatcttca ggcgcgcggt ctggcagtaa aaactatcca gcaacatttg ggccagctaa
    11941 acatgcttca tcgtcggtct gggctgccac gaccaagtga cagcaatgct gtttcactgg
    12001 ttatgcggcg gatccgaaaa gaaaacgttg atgccggtga acgtgcaaaa caggctctag
    12061 cgttcgaacg cactgatttc gaccaggttc gttcactcat ggaaaatagc gaccgctgcc
    12121 aggatataag gaatctggca tttctgggga ttgcttataa caccctgtta cgtatagccg
    12181 aaattgccag gatcagggtt aaagatatct cacgtactga cggtgggaga atgttaatcc
    12241 atattggcag aacgaaaacg ctggttagca ccgcaggtgt agagaaggca cttagcctgg
    12301 gggtaactaa actggtcgag cgatggattt ccgtctctgg tgtagctgat gatccgaata
    12361 actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc gccatctgcc accagccagc
    12421 tatcaactcg cgccctggaa gggatttttg aagcaactca tcgattgatt tacggcgcta
    12481 aggatgactc tggtcagaga tacctggcct ggtctggaca cagtgcccgt gtcggagccg
    12541 cgcgagatat ggcccgcgct ggagtttcaa taccggagat catgcaagct ggtggctgga
    12601 ccaatgtaaa tattgtcatg aactatatcc gtaacctgga tagtgaaaca ggggcaatgg
    12661 tgcgcctgct ggaagatggc gatctcgagc catctgctgg agacatgaga gctgccaacc
    12721 tttggccaag cccgctcatg atcaaacgct ctaagaagaa cagcctggcc ttgtccctga
    12781 cggccgacca gatggtcagt gccttgttgg atgctgagcc ccccatactc tattccgagt
    12841 atgatcctac cagacccttc agtgaagctt cgatgatggg cttactgacc aacctggcag
    12901 acagggagct ggttcacatg atcaactggg cgaagagggt gccaggcttt gtggatttga
    12961 ccctccatga tcaggtccac cttctagaat gtgcctggct agagatcctg atgattggtc
    13021 tcgtctggcg ctccatggag cacccagtga agctactgtt tgctcctaac ttgctcttgg
    13081 acaggaacca gggaaaatgt gtagagggca tggtggagat cttcgacatg ctgctggcta
    13141 catcatctcg gttccgcatg atgaatctgc agggagagga gtttgtgtgc ctcaaatcta
    13201 ttattttgct taattctgga gtgtacacat ttctgtccag caccctgaag tctctggaag
    13261 agaaggacca tatccaccga gtcctggaca agatcacaga cactttgatc cacctgatgg
    13321 ccaaggcagg cctgaccctg cagcagcagc accagcggct ggcccagctc ctcctcatcc
    13381 tctcccacat caggcacatg agtaacaaag gcatggagca tctgtacagc atgaagtgca
    13441 agaacgtggt gcccctctat gacctgctgc tggaggcggc ggacgcccac cgcctacatg
    13501 cgcccactag ccgtggaggg gcatccgtgg aggagacgga ccaaagccac ttggccactg
    13561 cgggctctac ttcatcgcat tccttgcaaa agtattacat cacgggggag gcagagggtt
    13621 tccctgccac agcttgatga agatctgagc tcttaattaa tgatcataat cagccatatc
    13681 acatctgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
    13741 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
    13801 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    13861 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgtttaaa ctaactttaa
    13921 ataattggca ttatttaaag ttacatatgg cggccgccct aaggctcgac ctcgaaattc
    13981 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
    14041 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
    14101 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
    14161 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
    14221 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
    14281 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
    14341 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
    14401 tcttccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
    14461 tcttcctcat ctccgggcct ttcgacctgc atccatctag atctcgagca gctgaagctt
    14521 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
    14581 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatcca
    14641 gatcgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
    14701 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
    14761 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
    14821 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
    14881 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
    14941 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
    15001 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
    15061 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
    15121 tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg
    15181 atgatatcag atccccggga tgcagaaatt gatgatctat taaacaataa agatgtccac
    15241 taaaatggaa gtttttcctg tcatactttg ttaagaaggg tgagaacaga gtacctacat
    15301 tttgaatgga aggattggag ctacgggggt gggggtgggg tgggattaga taaatgcntg
    15361 ctctttactg aaggctcttt actattgctt tatgataatg tttcatagtt ggatatcata
    15421 atttaaacaa gcaaaaccaa attaagggcc agctcattcc tcccactcat gatctataga
    15481 tctatagatc tctcgtggga tcattgtttt tctcttgatt cccactttgt ggttctaagt
    15541 actgtggttt ccaaatgtgt cagtttcata gcctgaagaa cgagatcagc agcctctgtt
    15601 ccacatacac ttcattctca gtattgtttt gccaagttct aattccatca gaagctggtc
    15661 gagtaacttt aaataattgg cattatttaa agttaggcgc gccataactt cgtatagcat
    15721 acattatacg aagttatgtc gagatattta aatattagac ccagctttct tgtacaaagt
    15781 ggttgatatc tctatagtcg cagtaggcgg ggcatggtaa tgagtttgat ggttgatttg
    15841 actggatagc ttcgatttgc atgtcagagg ccacagttct aacagaaaga taagactgtc
    15901 ctctttgggg agatgagagg agacagtggg gtggtttaaa ggagaggtgg ggcctaagga
    15961 agagacggtc agagcagcag atcagagtgg acagggaagc ttagggaatg gctgggcctt
    16021 gaagaaggag aggctcctga gagcgtggct ggggaggccc aacagaaggg ctagagcgaa
    16081 gctgtgggca ccaggagcaa ccttgtactc agatcgggga gaccggctgg gtctctgtgt
    16141 gggtcacctg gccttcttgt catctccctg aaggatgctc tggaggacac tagggattct
    16201 gaaatgaagc tgaaggcatt cccggaagtg tgtaacgaga ccatgatggc cctctgggag
    16261 gagtgcaagc cctgcctgaa gcatacctgc atgaagttct atgcacgtgt ctgcaggagc
    16321 ggctcagggc tggtcggcca gcaggtgaga aggagagatg tccacagcct gggttctgga
    16381 tggggaatgt ggagaatcca tgaaataggc ctcattccat atgctgggta cagttgatta
    16441 gcattgcttg actgcacatc gttctagtgt catggtcgat tggtgatgtc tgcttggcag
    16501 gtaccatctc tttcaagacc tttaactggt tgggtaaaat ggccttatgg gatggtagaa
    16561 tcctgtgtta gaataggaaa agggaattaa aaaatattta gtctatttgc attaattcta
    16621 tccctacctg tgagattcac ttggttggaa ctcagtaaaa ttcactgttt cctcaaggct
    16681 gagcagctaa gcaggagtgg ctatttattc tttatggccc aaggtgagac ttgggaacct
    16741 ccgagtttcg ccatgagaaa tggatttggg gggcggcggc ggggggacgg ggggtgctga
    16801 cagggtaccc aggaggcaga acagtgggaa cttgggtcag tttctgtgtg gccacagggg
    16861 gatacaggct ggagtacaca caatgcctta ctctacttct ctgggtccca gctagaggag
    16921 tttctaaacc agagctcacc cttctacttc tggatgaacg gcgaccgcat cgactccctg
    16981 ctggagagtg accggcagca gagccaagtc ctggatgcca tgcaggacag ctttgctcgg
    17041 gcatctggca tcatagacac gctctttcag gaccggttct tcgcccgtga gcttcatgac
    17101 ccccactact tctcccccat cggcttccca cacaagaggc ctcatttctt atatcccaag
    17161 tcccgcttgg tccgcagcct catgtctccc tcccactatg ggcctccgag cttccacaac
    17221 atgttccagc ctttctttga gatgatccac caggctcaac aggccatgga tgtccagctc
    17281 cacagcccag ccttccagtt cccagacgtg gatttcttaa gaggttagaa agcaattgct
    17341 ttctttctaa gtaggaatgt gaatgtccca gatgtctagg ctcttccagg gcttgacctg
    17401 tgctggtttt atgttcctag aagcctcaga ggctggtatt tcctccattt gtagggtaaa
    17461 ggagctagga tgggggcaaa gaaatgcagg taaatgtggg ggggcgcagt tcaattagca
    17521 tttgtagaga ttggagctga gatggcgcaa cgcaattaat gataacttcg tatagcatac
    17581 attatacgaa gttatggtct gagctcgcca tcagttcatc ccagatatta tttcttggga
    17641 acttttgagg gtgtggtgtg agaggcccgc ccttccaggc aatgagtctg tgtcatctta
    17701 aagaagatgt tcatggctgt gaccttagcg tgtaatgcct gtaaggatga aatatcacgt
    17761 ggacacgaga aaccggtgtc tgaggagtga tttttttttt ccattagtgc cttgggtctt
    17821 aataccatgt cctacatggt atggtttgca ttagaaatag aagaagtctt ggcatccaac
    17881 acacatctac tgtagctcca ttgtctaaag atggtttatg ttgtcaggac acacttgtgc
    17941 tctctggggc ttgaaggcca gttagggagt accatcagtc aaggagaaca aagaacagcc
    18001 atgatgtcag aaggcacaca gctgagggga tgtggcagga gagatctcct cagatggaga
    18061 tggtgggaag gcaggcagga cctgctgaga gccgtgggaa tatcctcagc cacagggaag
    18121 tcccatggct gcaagcaaac aaataagctg catcgtgccc tgaccatggc actgagcaga
    18181 ggtgtggaag gctgtcgggt gggcatcctt ctgatgtgcc atgtgtctaa tcctgcccct
    18241 ctttcccctc atgtgtccag aaggtgaaga tgaccgcact gtgtgcaagg agatccgccg
    18301 caactccaca ggatgcctga agatgaaggg ccagtgtgaa aagtgccagg agatcttgtc
    18361 tgtgggtgag tccgggaacc agtggtcatc tgactgtggg tctccagtga ctcactggca
    18421 ctatcctttt ccaaagacta gctctctgcc tgggaactct taatccccgg gagactcctc
    18481 accctggtgc acagcctact ctcctcttat ctagagcttc attatttggg agctggggta
    18541 ggacccagtg cctcctgcat gttaggtgaa cgctctacca ctgagccaca tcaattttac
    18601 tcagaatcaa gttcaggtgc agattgttct catatagatg aggtggagct aaagtgccca
    18661 ccaccacccc tgtggctcac tggcaaacct gcagtccacg gtggaattac actgtatcga
    18721 ccagtaagcc acagctcgtt cccctggcta ctttgttcca cagtgccaag tactgtagac
    18781 cctgattttg aacttctgat tatgaaatct ttgacaacat gcgttttgtt taacaagtgc
    18841 tcgccacatg ccaggcactg ctgcaaacac gtcacaggtg tctgttcgca gaatgatcct
    18901 gtgagctggg tacagctctc tttccgtctt gctggatggc taagtgactt atctaagatt
    18961 acgtgacacg aggcttctca cttaggctgt gctcataaac agcaccctgg ggtttctccc
    19021 tggggacctt gagctatgtc cttgtgatgg tggtccttgg tatcctggcc gcattctttt
    19081 tggtgaacta gtccctcaga aaaggaacta tttttccttc atcagttccc caggcaatct
    19141 gggtatggtg acatatccct ataatcccaa catttgggag gctgaggtag gagggtggtg
    19201 aatccgaggc cagcggcgag gttctgtctc aatgaaaaca aaacaaaata cagcaaaccc
    19261 aaaactgttc cccacagatt tgtcaggtct ggctatcaag ccaatgcccc gccttcagcc
    19321 tggtctttac agtggctcac agtacacggt gctacctgct cagatcaccc tctttgttgg
    19381 ccgggacaga cctttagagt ctggttaaat attcccagcc tcattcctgc aatgttagga
    19441 ggctaggaaa aaaaaaaaaa tactagtgct ttcaaaggtc atagtgtgac attataaaca
    19501 aatatgggcc acacctgtag gctttccctt atagttgaaa gatggggtgc ctgagcacag
    19561 agaagccagt caccttatag actggatctc ttacatcaga cttagcgatc ctctcaacag
    19621 tcacccgtta gctcttattt acggtgctta taatggtatc ctctcagcag tcaccgtttc
    19681 tgtcacccgt tagctcttat gtacagtgct tacaatggta tggataggct tcacagagac
    19741 ctctcgcctg ggacatcttg cctcagccct gtgggcagac ctgaccatgg ctgagcattt
    19801 aacaacattt ttggctccaa cctaccatct accaagagca accctccctc cactgtgaca
    19861 atcagaaaca tcttcagatt gtctggactg tctcctggtg ggtggtgtgg tcccaggttg
    19921 agaaccaccg ttctactctg tccttctctc aggactttct tgtttgggct aatcagtggt
    19981 tagagtgctc cggttattcc tggaagattt cttctcattt ctgcttctgg tctccaatcc
    20041 tgtgtcttgc cccatgcaac attatatctt attttaagta tgaacaattg tagtagccac
    20101 atagggccca gaaggcatct ctcttgtctt gcttagtttg gatatagcct ctcatagatg
    20161 tttaaaaaat aattgaggag gggggaggcg ttgttaaaca ctggttttca gaaagcatga
    20221 gcactttgtt ggtctcagta gcctgatgcc tgcagtgcac tcttgcagaa gagaggatgc
    20281 cagagttcct atttcctcct ggtcatcctg ggtggacaag ggcaggcaag gactgaccct
    20341 tgggcaccaa acacctgtgt tttgtagact cgggacctgg ctgtctcttc tgcaggtttg
    20401 gttctgggaa aatggtcttg ccgaatctgt caaattatgt tcaaaaataa gtttggagaa
    20461 tattctagaa ccctggtctc aggggctcag gttgaggagt gaaagatggg gcttcaatcc
    20521 tggttccaca gatcgcttcc tctatagatc tgagcaaggt ccacctaagt aaataatgat
    20581 cctgttcttt gtggggtgtc cagtgccaca gtagcttcct cctattttgg aaatgagtcc
    20641 tgggaggagc tcagggagca agagtaagtc caggaacatg gggtagtcag cataggtgct
    20701 ccccagagca ccgtcagcag cattgtgaca ggtcaccaag actggtcatg tctaccgccc
    20761 aaagaagact cagccacgct gctctgacac agtttagcac agctaagcca ttgtatccca
    20821 aagcacgaag ggggcagcac cttggtttcc gtgtccgggg aactgtaaga gctgatctgt
    20881 agccctccct ggaagagttc ctctgcttca gattgtggct gctgcagctg gttcccaggg
    20941 gcgtgtccgt cccctttacc ctccctttca tgcttctgcc ttctgcttct ttcttctctg
    21001 ggcagactgt tcaaccaaca atcctgccca ggctaacctg cgccaggagc tgaacgactc
    21061 gctccaggtg gccgagaggc tgacagagca gtacaaggag ctgctgcagt ccttccagtc
    21121 gaagatgctc aacacctcat ccctgctgga gcagctgaac gaccagttca actgggtgtc
    21181 ccagctggct aacctcacac agggcgaaga caagtactac cttcgggtct ccaccgtgag
    21241 ttgtatccca gtcatttgat gaggctgaga cccgggtgtg atccagaggt gatgtgtgtc
    21301 tcttagagat gtggtgggac aaaggtccca cctaggattt acaccagcag ggactaggaa
    21361 aaccgtatgc acgttgtagc attgaaatgc ctttgccccg tcaaatatcc aagtctcatt
    21421 gtcaagatga ggaaagagag aggttctgtg gtttgctaag aacagacagt tttgggcaac
    21481 ttccctgggt cagcctgtca gcctggctgt aaagctgctt cctgcccagg gccactctga
    21541 gtgccacatg aattggttca ctgttagcca gtgagaactc ggtttccttt agtttgtgga
    21601 ccccacccct accccctttg accacagact ggctaacatt tgtacaaagc aaatgttggg
    21661 gccaggtgtt gtggtgcgtg cctgtaagcc agcactgaag aaactgaggc aggagagtag
    21721 agagcttgag cctgggctgg actatatagt gacagcctct ctcaaacaaa gcaaaacaag
    21781 agaacacaac aagaagaaaa cattcaaaaa tcatttcaag tttctggagt gcctacttgg
    21841 ctagtc
//