LOCUS       114839#pL1L2_GT2_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20427 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT2_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 114839
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000048471
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..9493
                     /note="5 arm"
     misc_feature    9521..9545
                     /note="B1 Gateway"
     misc_feature    9558..9605
                     /note="Frt"
     misc_feature    9606..10105
                     /note="mouse En2 intron"
     primer          complement(9674..9695)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9570..9593)
                     /note="FRT3"
     primer          13764..13782
                     /note="seq primer NF"
     misc_feature    13824..13831
                     /note="RE sites"
     misc_feature    15624..15657
                     /note="loxP"
     misc_feature    complement(13832..13863)
                     /note="Rox"
     misc_feature    complement(15584..15615)
                     /note="Rox"
     misc_feature    13864..13885
                     /note="polylinker"
     CDS             14444..15040
                     /note="puromycin N-acetyl transferase"
     misc_feature    14559..14564
                     /note="Mutated RsrII site"
     intron          10106..10480
                     /note="mouse En2 intron"
     misc_feature    10478..10480
                     /note="Splice Acceptor"
     exon            10481..10527
                     /note="mouse En2 exon"
     primer          10381..10402
                     /note="seq primer FCHK2"
     misc_feature    10528..10650
                     /note="1Dleader"
     misc_feature    10651..10702
                     /note="F2A"
     misc_feature    10798..11514
                     /note="EGFP"
     misc_feature    10708..10797
                     /note="3X NLS"
     misc_feature    13580..13818
                     /note="SV40 polyadenylation site"
     misc_feature    12613..13557
                     /note="ERT2"
     promoter        13896..14407
                     /note="PGK promoter"
     misc_feature    15151..15583
                     /note="PGK pA"
     primer          13638..13661
                     /note="LRPCR RAF5"
     misc_feature    11524..11577
                     /note="T2A"
     misc_feature    11574..11574
                     /note="A to G to kill RsRII site"
     misc_feature    11578..12603
                     /note="Cre"
     misc_feature    10496..10528
                     /note="FCHK lf2a_lacz_gt2"
     misc_feature    10496..10511
                     /note="FCHK QC prefix region"
     misc_feature    10512..10522
                     /note="FCHK QC critical region"
     misc_feature    10523..10528
                     /note="FCHK QC suffix region"
     misc_feature    15680..15700
                     /note="B2 Gateway"
     misc_feature    9494..15730
                     /note="Synthetic Cassette"
     exon            16082..16263
                     /db_xref="ENSEMBL:ENSMUSE00000330829"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 1 ENSMUSE00000330829"
     misc_feature    15731..16418
                     /note="Critical Region"
     misc_feature    16442..16475
                     /note="Downstream LoxP"
     misc_feature    16419..16498
                     /note="synthetic loxP region"
     exon            16845..16900
                     /db_xref="ENSEMBL:ENSMUSE00000330823"
                     /rank="6"
                     /note="ENSMUSE00000330823"
     misc_feature    16499..20427
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatatttc agttgcttat tggtataaaa ttagagttct ttcttttgat
     4321 cattcaagag tgatattaac ttttattatt gtgatttcct tttgctccta gacttcctga
     4381 gaagtgggta tttcccctgt ctgtggattc ccacttgatt gaaccttgag tttgtggctc
     4441 atcttagtaa tctgtggcag tagggcccac gtccctaggc cggcagtaaa cacgagctaa
     4501 acagttaagc aggaaactct ccttcctttc cgttctgtgt cactatcacc tcaaaagaac
     4561 ctgacaaaat tgagtgatgc ctttcttcta gaaacgtcat ctgacttctc ttactacttt
     4621 gggaggtatt agagtccccc ttttatacta attttaatat ttttttttta tttcattttt
     4681 ttagtgtcac actggttttg cttaagagaa gtacttatat ctgtgtccta atcttttgct
     4741 tctctgttcc cccatataca atcccaacca aagcatttct ttacactcca tatcattcat
     4801 gacagcagca tgctggccag gttccttggc agttttccat accttagtcc cccagaccct
     4861 cagtaatgct gggcaaatct agactgatca ctgtcagctt gctcctccat ttcaaattca
     4921 ccttcacttc tgctcacatc tctctcctct aagaatcacg tttctgactt cagtactcct
     4981 gattccattt cttctttcat actttaagct ttgggtctct tctcttttcc agttgcacct
     5041 ttatggccgc tggtttcccg cctattgctt gcaaatagac tcaagattta catagccagg
     5101 ctcgccccaa gaaaacatgt cttgattctg tgctgtcttc catatttctt tccaacctcc
     5161 attcttaatc ctgaggctac tcttatgttc ttgatttctg catcatcact attccttctc
     5221 actggttcct tcccccttcc tactgtctca ccaaatcttc ttagtcacca gtgagttatt
     5281 aattactaaa tccagtagaa ttttatcttc tctatagaat tattggccag atgatcagtt
     5341 aaaaaattac ccacaagcat tcaaaaataa tatacttgcc cttaaatgct ttgtaagaat
     5401 agaaagatga gtataagaga aataacatat ggaaacatac atggatctga tcaaaagatt
     5461 agacatgaag ggagacagga cacatacatg aatctgatca aaagattaga catgaaggga
     5521 gaagagactt gcaagttcac agaaatgtgg gctatgcaga ctggactcag ggacaatttt
     5581 aaggtagacc tgaaacttat ggaaacttag atcattcatt gtattttctc attagattta
     5641 agtctgctaa agatgccata ctccagacta gaaagggacg tggatatttt tatcattaag
     5701 aagtggaaga aagtaggtta tgcttatagt gaaacacaat gccagggttc agagactgtt
     5761 gagtttatta ttgctgtggt aggtctcaaa acacctccac aatttccttg ggagatcaaa
     5821 ggaaaagttg atgtgcttac aaagtaagaa gcttggataa attaccaagg aggttacttt
     5881 ggagaaaagg agattgtatt ttatctcctg aagttactgc gaaggaggta gggatgcaca
     5941 ggaggatgga gcatgggcag aatctctggg ggtggggcta cagtcactat aaagaacatt
     6001 agcaaaggga tgttaggagg aatccttaga tacagagtag ggaccattat ataggtccca
     6061 tgttttggga aataacagtt gtgtcaaaat gcaattcttg ctgtaaaagt gtagtctaca
     6121 gcatggctaa tgatgggaga gtttgcaagg aatacattaa ttttgatgtt ttctactttc
     6181 cccctaagta ttgttcactg ccagctgcta catcataaat ttagattttt ttctttttgc
     6241 ctcagggagg gttttagaat agaatatcca atctgctgct gaaaaaaatt gtataaagag
     6301 tctcaaaaga tgtttagttc caaacttttt gagaaaattt attcctatga agaaaaagcc
     6361 cctattaggc catgctattg ttggcagtgg gattatggtc aagccaccgc ctacttttga
     6421 gggtcccatg attatttttt ttaagaggct tttattgtgt agccgagggt ggcccagggt
     6481 agccctcatg atcctccagt ttagtgcttt gattacaagt ggacatcact atgctccacc
     6541 ttgctgtctg tactttaaaa gacccattgc cttcctatgt ggttgtgctc aaacttaatc
     6601 atccacaaaa atctcacaga agcaacattt gactctgggt gatctcagac aatgaggaga
     6661 atgttggcta tgcattaagg tgaatacata gtaaaataac aagttttttt taaaaagcct
     6721 tgagcagcac taaactgact cttctctgat tctgggtcac ttaatgtttg ctttcctttc
     6781 cttttgccaa gctgcagagt agaaagggaa tttagagcag accagaaagt atagcttagg
     6841 actcagaaaa gaggatgaag gatttgattg tcatccagct ggagctgccc cggggcctct
     6901 tctaattcca taaagtcttg ctttgctaag gctttattga cagacaaact gaaatcaaaa
     6961 agaatatgaa gccttctgca acacaagctt ttattatgtt ttgcctcagc ttgtgacaag
     7021 cttagggaaa tggtttattt tgtttagtgg gcctcaggaa aagtgaattt gcaaaatgta
     7081 agtataaacc caatagctta aattgttctt tgctgatgat tgaaaaactt tgctgagagc
     7141 cctggcaatc taattttaaa atgacctgag gagaagctcg gcatctatcc tgttcattgc
     7201 acaatcttaa tggagcacct acggtgtgtc aggttataaa gcagtgccca ctctcaagag
     7261 cccatagttt gttttaaaat caagaagaat ttactacact acttccagaa taacaccttc
     7321 tctcttctgt aagacttaga agctgcctac cttattcact tttcaattta tacagtgctg
     7381 agaaaggtca gtttgaatgt tacagaggaa gggaggcaca gcggcttgta tacataaaga
     7441 atttggtata agagagggtg ttgtaattga aacagatgaa cattacagga tgaatggttt
     7501 ttaatgtgaa gctgagggtc tttgaagacc atggagcatt ctctcctatt gtcttcattg
     7561 gcaggctata caccactaaa ctgattagaa agccttgggt ctgtccccag cagctcagct
     7621 agaaatggga tcttgattca taactggtgt taagtcctca ggaatatatc ctatcggggg
     7681 agggggggtt ggagttgtaa ggtctcatct gctttgtttt tagcttcttg acatttctct
     7741 aaggtccctt aagagccagg aaggggtgga aagaaggacg acaacaaaaa tcccatgtag
     7801 catgcttgtc aagtgatgca tttcataaaa cactgttgag attttactta taagacacag
     7861 aggactccta tcctataaca ttggttactg gtagcaataa gcactccggc tggaagagcc
     7921 accttcactc attgggttct ttgctatttt ggaaaggagg attggagccc aagattagtt
     7981 tttgctggaa aaaagcaagc taacgactgg ctgcaagcat gtccaggctt tgaaactact
     8041 aagaatgtag gctgcatata caaaaactct cccttccccc agttcctaaa atccagagcc
     8101 tcttctgact tgatggacct gggatctggg cacatattct cccccacata cacttaggct
     8161 taacctttgg cttaatagct cctggcacta tctgaaaatc ctgttacttt taaacaaagg
     8221 ttctgccttt ttttgctttg caaagtttac aacatatgtt caatcctggt ccccacttct
     8281 taagattcta ggcagatgta cttgtatcat caagaatgtg tgcctagaga aggtagtgaa
     8341 tgttaaagta aacttagtca tcagtgggta tctggcaaat gcattgagcc tgagtaaatg
     8401 agtgacctta aaatcctaaa taaaaccagt taatctgaat cttagctaat ttagctttaa
     8461 aaggtgtgga gtaggggaat cactctaaat tttgagaaaa aataaaagta gtttaaaagc
     8521 tctaaaatag catgcatgca tacatacata catgcatata tatttatctt gatggtttcc
     8581 atcatatatg caacaactat tttgttatat tggcttaaaa tatttattta aaaatataaa
     8641 ggaatcaatt acctgtcagg atcacctctg ataccagcta tctctgcttg ctcactagta
     8701 atcgaagctc aactgtaaat atgatattct ccccctatct gttacctctt tataatacta
     8761 ggaataatat ggagattttt atgttcataa ttctataaat gatgtgaacc agtatacttc
     8821 attttgcagt tttatttttt gctatatcag tttattccct tcggtctgta gactagtgtg
     8881 aaacttgctc tcccaacctg cagcctggct tactgtaagg gtagttaaaa ccatacccag
     8941 tgcagctttc tttggtttgt ggttctacta ccatccaagg taaaggtcac cagctggcaa
     9001 ttctaggaaa gccccacgat ggtttacatt cataaaacta gattaataat ttctaccatt
     9061 caaggtatcc ttgagatcgt tacatgagac agttaaccct gcaaagccta ctgctggaga
     9121 ttgtcttact atggcatcta aactggagca ctgagtggcc tgaagcagta tgtaaatacg
     9181 acgataaatg tatgctttgc agtcttactg tatgtttagc ttaactatca attttgcttg
     9241 ctaaattaaa aataattgtg ttttactctt aaaaggtagc cctgtatata ccataaattc
     9301 tcaggtttgt cttttaccaa attggaaaaa tattactttt aatactccct ccatggaata
     9361 agaaggcttg aattcttcaa gtaaggaaaa gaccacacta tttataatac tttgtttctg
     9421 ttctctatgc agctggaaag ggatggatag ctagcatttt tctctttcat gttcctaagt
     9481 agctaataag tgtaaggcgc ataacgatac cacgatatca acaagtttgt acaaaaaagc
     9541 aggctggcgc cggaaccgaa gttcctattc cgaagttcct attctctaga aagtatagga
     9601 acttcgaacc ctttcccaca ccaccctcca cacttgcccc aaacactgcc aactatgtag
     9661 gaggaagggg ttgggactaa cagaagaacc cgttgtgggg aagctgttgg gagggtcact
     9721 ttatgttctt gcccaaggtc agttgggtgg cctgcttctg atgaggtggt cccaaggtct
     9781 ggggtagaag gtgagaggga caggccacca aggtcagccc ccccccccta tcccatagga
     9841 gccaggtccc tctcctggac aggaagactg aaggggagat gccagagact cagtgaagcc
     9901 tggggtaccc tattggagtc cttcaaggaa acaaacttgg cctcaccagg cctcagcctt
     9961 ggctcctcct gggaactcta ctgcccttgg gatccccttg tagttgtggg ttacatagga
    10021 agggggacgg gattcccctt gactggctag cctactcttt tcttcagtct tctccatctc
    10081 ctctcacctg tctctcgacc ctttccctag gatagacttg gaaaaagata aggggagaaa
    10141 acaaatgcaa acgaggccag aaagattttg gctgggcatt ccttccgcta gcttttattg
    10201 ggatccccta gtttgtgata ggccttttag ctacatctgc caatccatct cattttcaca
    10261 cacacacaca ccactttcct tctggtcagt gggcacatgt ccagcctcaa gtttatatca
    10321 ccacccccaa tgcccaacac ttgtatggcc ttgggcgggt catccccccc cccaccccca
    10381 gtatctgcaa cctcaagcta gcttgggtgc gttggttgtg gataagtagc tagactccag
    10441 caaccagtaa cctctgccct ttctcctcca tgacaaccag gctcccaggt cccgaaaacc
    10501 aaagaagaag aacgcagatc gcagatctct agagtcaccg agttgcttta ccggatgaag
    10561 agggccgaaa catactgtcc aaggcccttg ctggcaatcc acccaactga agccagacac
    10621 aaacagaaaa ttgtggcacc ggtgaaacag actttgaatt ttgaccttct caagttggcg
    10681 ggagacgtcg agtccaaccc cggaccggtg catgtggacc ccaagaaaaa gcggaaggtg
    10741 gaccctaaaa agaaacgcaa agtggatcct aagaaaaaaa gaaaggtgca cgtgtccatg
    10801 gtgtccaagg gcgaggaact gttcaccggc gtggtgccca tcctggtgga actggatggc
    10861 gacgtgaacg gccacaagtt cagcgtgtcc ggcgagggcg aaggcgacgc cacatacgga
    10921 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccttg gcctaccctc
    10981 gtgaccacac tgacctacgg cgtgcagtgc ttcagcagat accccgacca tatgaagcag
    11041 cacgacttct tcaagagcgc catgcccgag ggctacgtgc aggaaagaac catcttcttt
    11101 aaggacgacg gcaactacaa gaccagggcc gaagtgaagt tcgagggcga caccctcgtg
    11161 aacagaatcg agctgaaggg catcgacttc aaagaggacg gcaacatcct gggccacaag
    11221 ctggagtaca actacaacag ccacaacgtg tacatcatgg ccgacaagca gaaaaacggc
    11281 atcaaagtga acttcaagat ccggcacaac atcgaggacg gctccgtgca gctggccgac
    11341 cactaccagc agaacacccc tatcggcgac ggccctgtgc tgctgcctga caaccactac
    11401 ctgagcaccc agagcgccct gagcaaggac cccaacgaga agagggacca catggtgctg
    11461 ctggaattcg tgaccgccgc tggcatcacc ctgggcatgg acgagctgta caagcctgca
    11521 ggcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cgggccgtcc
    11581 aatttactga ccgtacacca aaatttgcct gcattaccgg tcgatgcaac gagtgatgag
    11641 gttcgcaaga acctgatgga catgttcagg gatcgccagg cgttttctga gcatacctgg
    11701 aaaatgcttc tgtccgtttg ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa
    11761 tggtttcccg cagaacctga agatgttcgc gattatcttc tatatcttca ggcgcgcggt
    11821 ctggcagtaa aaactatcca gcaacatttg ggccagctaa acatgcttca tcgtcggtct
    11881 gggctgccac gaccaagtga cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa
    11941 gaaaacgttg atgccggtga acgtgcaaaa caggctctag cgttcgaacg cactgatttc
    12001 gaccaggttc gttcactcat ggaaaatagc gaccgctgcc aggatataag gaatctggca
    12061 tttctgggga ttgcttataa caccctgtta cgtatagccg aaattgccag gatcagggtt
    12121 aaagatatct cacgtactga cggtgggaga atgttaatcc atattggcag aacgaaaacg
    12181 ctggttagca ccgcaggtgt agagaaggca cttagcctgg gggtaactaa actggtcgag
    12241 cgatggattt ccgtctctgg tgtagctgat gatccgaata actacctgtt ttgccgggtc
    12301 agaaaaaatg gtgttgccgc gccatctgcc accagccagc tatcaactcg cgccctggaa
    12361 gggatttttg aagcaactca tcgattgatt tacggcgcta aggatgactc tggtcagaga
    12421 tacctggcct ggtctggaca cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct
    12481 ggagtttcaa taccggagat catgcaagct ggtggctgga ccaatgtaaa tattgtcatg
    12541 aactatatcc gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc
    12601 gatctcgagc catctgctgg agacatgaga gctgccaacc tttggccaag cccgctcatg
    12661 atcaaacgct ctaagaagaa cagcctggcc ttgtccctga cggccgacca gatggtcagt
    12721 gccttgttgg atgctgagcc ccccatactc tattccgagt atgatcctac cagacccttc
    12781 agtgaagctt cgatgatggg cttactgacc aacctggcag acagggagct ggttcacatg
    12841 atcaactggg cgaagagggt gccaggcttt gtggatttga ccctccatga tcaggtccac
    12901 cttctagaat gtgcctggct agagatcctg atgattggtc tcgtctggcg ctccatggag
    12961 cacccagtga agctactgtt tgctcctaac ttgctcttgg acaggaacca gggaaaatgt
    13021 gtagagggca tggtggagat cttcgacatg ctgctggcta catcatctcg gttccgcatg
    13081 atgaatctgc agggagagga gtttgtgtgc ctcaaatcta ttattttgct taattctgga
    13141 gtgtacacat ttctgtccag caccctgaag tctctggaag agaaggacca tatccaccga
    13201 gtcctggaca agatcacaga cactttgatc cacctgatgg ccaaggcagg cctgaccctg
    13261 cagcagcagc accagcggct ggcccagctc ctcctcatcc tctcccacat caggcacatg
    13321 agtaacaaag gcatggagca tctgtacagc atgaagtgca agaacgtggt gcccctctat
    13381 gacctgctgc tggaggcggc ggacgcccac cgcctacatg cgcccactag ccgtggaggg
    13441 gcatccgtgg aggagacgga ccaaagccac ttggccactg cgggctctac ttcatcgcat
    13501 tccttgcaaa agtattacat cacgggggag gcagagggtt tccctgccac agcttgatga
    13561 agatctgagc tcttaattaa tgatcataat cagccatatc acatctgtag aggttttact
    13621 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    13681 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    13741 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    13801 tgtatcttat catgtctgga tccgtttaaa ctaactttaa ataattggca ttatttaaag
    13861 ttacatatgg cggccgccct aaggctcgac ctcgaaattc taccgggtag gggaggcgct
    13921 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
    13981 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
    14041 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
    14101 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
    14161 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
    14221 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
    14281 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcttccggag gcccggcatt
    14341 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
    14401 ttcgacctgc atccatctag atctcgagca gctgaagctt accatgaccg agtacaagcc
    14461 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
    14521 gttcgccgac taccccgcca cgcgccacac cgtcgatcca gatcgccaca tcgagcgggt
    14581 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
    14641 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
    14701 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
    14761 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
    14821 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
    14881 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
    14941 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
    15001 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
    15061 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg atgatatcag atccccggga
    15121 tgcagaaatt gatgatctat taaacaataa agatgtccac taaaatggaa gtttttcctg
    15181 tcatactttg ttaagaaggg tgagaacaga gtacctacat tttgaatgga aggattggag
    15241 ctacgggggt gggggtgggg tgggattaga taaatgcntg ctctttactg aaggctcttt
    15301 actattgctt tatgataatg tttcatagtt ggatatcata atttaaacaa gcaaaaccaa
    15361 attaagggcc agctcattcc tcccactcat gatctataga tctatagatc tctcgtggga
    15421 tcattgtttt tctcttgatt cccactttgt ggttctaagt actgtggttt ccaaatgtgt
    15481 cagtttcata gcctgaagaa cgagatcagc agcctctgtt ccacatacac ttcattctca
    15541 gtattgtttt gccaagttct aattccatca gaagctggtc gagtaacttt aaataattgg
    15601 cattatttaa agttaggcgc gccataactt cgtatagcat acattatacg aagttatgtc
    15661 gagatattta aatattagac ccagctttct tgtacaaagt ggttgatatc tctatagtcg
    15721 cagtaggcgg atttaatata gttatctgac tgaagtctca tgtgtattta gaagtttgag
    15781 ggagtaggat tgacaacttg gcaattaaca gctcttactg ccctatcacg aggaccagag
    15841 tttaggtccc agcatggatg taacaagcca catattccac aagcacctgt aatcccaggc
    15901 ctgagagata cagcaatgtt ttctgggtcc tgtgtacaca cagacacaca agtacctgta
    15961 tacacatgtg cacatacacc aatatgtgaa tataagccta agcaaaatga accttagaaa
    16021 cttgcacttg catttttcat atcactgtgt tttcaaacat catattttca ttattcaaca
    16081 gattttatac aattcgatat agagaaaaag ataaagaaaa gaaatggatt tttcaactct
    16141 gcccagctac tgaaacaatt gtggaaaatc taaagcccaa cacagtttat gaatttggtg
    16201 tgaaagacaa tatagaaggt ggaatttgga gtaagatttt caatcataag actattgttg
    16261 gaagtaagta tttatttggt ccaataaagt atgttttaat tcttttttgg gggggggggt
    16321 ctggcttaaa tggtttcact agctgtgtaa tatgaaatga gttcactttc agttattttc
    16381 aatagcttac atgttctagt atttaaaaaa atacaaagga gatggcgcaa cgcaattaat
    16441 gataacttcg tatagcatac attatacgaa gttatggtct gagctcgcca tcagttcaaa
    16501 tgtgcttttt aaaaataaac aatgaatgct attatgtttc cagctatatc actgtagtac
    16561 tttaaaagat atatttgacc tatgcacagc agctgagctg aacaaataaa atgtcaatat
    16621 gtgtaaacat cacatgggaa ggtaagccag tggagtgaac acagcctacc tgaggctcga
    16681 acttctcttc cttacctttg ggctcacaca tcattggagt taatatctga aatgatagta
    16741 aatgaaacaa aaaagaccat gacttggaat tgtccccaca gtgagctaga actgattaag
    16801 gagcagaaat aataaaagta atgacatttc cttcttattt gcaggtaaaa acaaagtaaa
    16861 cgggaaaatc caaagcactt atgaccaggt ccactcagtg gtaggtgcca atttattaag
    16921 atttttcttt aaagataatg taattgtgtt tctacttaga ggaagggaac agccctgctg
    16981 tgagatacca agattatcta ctggacactg gggaaaatgc ctaatgattt aggaattagc
    17041 tgcttttttt ttcttgcaaa taatagacat atgctcctag acccttccta acttgtttaa
    17101 agctctccaa tttagagact cgatgcagtc attctagtat ttgtatattt tagaatgttt
    17161 tgagattttt gttcattctg tcttgtgaat attctttaaa gatgtgttca tttagtgtgt
    17221 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgaga gagagagaga gagagagaga
    17281 gggagagaga gagagagaga gagagattat tcatgtatac cacatgacag gtacctgtgg
    17341 aggccagagg gcatcatatt ccctggaacg gaggtaaggg cagctgtgag acatataact
    17401 cagaattcag tccaggtcct ctggaagagc agcagttgtt actaaacact gagtgggctg
    17461 tttttcatac aaaactttca gatactgact gatagctaca tgtcccaggt atattgataa
    17521 agttcaaatc tttttatgtt ttgaaatgat gaaacaacca gcttggtttt gtccagcttg
    17581 gttttgaaat gcccaacctc cagtcagctc tataaatgtt tgtatgtttt gagtgtaaca
    17641 gtttcccacc tccaatactt acagattttt tttagattta tttatttatt atatataagt
    17701 acactgtagc tatcttcata cactccagaa gagagcatca gatctcatta tggatggttg
    17761 tgagccacca catggtttct ggtatttgaa ctcaggacct ttggaagagc agtcagtgct
    17821 cttaactgct gagccatctc tctagacccc caactttttt ttttgaagat ttatttatta
    17881 tatataagtg tactgtagct gtcttcagac actccagaag agggcatcag gtctcattat
    17941 ggatgattgt gagctaccat gcagttgctg ggaattaaac tcaggatttc tggaagagca
    18001 gtcggtgctc ttaactgctg agccatctct ccagccctca cagaaattct tagctcttca
    18061 caccatcacc attttctctc agttcagtaa atggtcctca caaatcatct tatcaattat
    18121 gtataatggg aggcatggtt aaagtcatca gtgagagaga tggtttgtat ttcacatcac
    18181 atactcaggt ataaggtcaa aggtgctact ttattgagca ggtagaagag gcactgggtg
    18241 agagaatgag aatttggtac tgacttgagt tgccatgtgt tagaattata cagcttgaga
    18301 aggagccctt cagtcaaatg gacataaaga gggcttagtg tgatttttct catttttcta
    18361 atgatataac atggaccctg ctagtccctt ctctcttatt tcctgactcc cttatcctgg
    18421 gaatggttcc tttttacttt ctttccacct gtacctccag gttgtttact ctcatctgaa
    18481 gatttgaaac taggagcctc caatgagaaa gactatatat accatgttta tttttttggg
    18541 tctgggtcta actgaatata gtcatttcta gttccatcca gttacctgca aagttcatgg
    18601 tttcctttct tctccaaggc tgaatagtat tttatagtgc ctgcgtacca cattttcatt
    18661 atcagtttgt caatatcagg atattggggt gtctctattt cctgcctgtt gtaaatagag
    18721 gggcaacgaa catggctggt tgagtatctg tggcgtcatt tgggtacata tgccacggag
    18781 ttagcataac tggatcgtat ggtagacttg ctttgagcat actgaaatcc tccacattga
    18841 tttccacagc agctggacca gtttgcagtc ccactaacag tgaatgaagg ctcccttttt
    18901 ttcaggcacc cctctagcat ttggcagttg ttttgatgat cttctccatt ctgactggaa
    18961 taagatggca tctcaacgtc gttttgattt gcattttcct aattgctggg gacaatgaaa
    19021 tttttttttt aaatctcagt aatatgcttt ttataaaatc aactatggca gatttttata
    19081 aaattggata ctccaaattt tgttgcatat gtgtttaaga tagtgatgac ttcttacttg
    19141 ttccctgatg agaatagcat gccctttatt tttcctgagc agcctcagtt tgacttagtc
    19201 taccccctca gaccatagga tagtgatgct ttttcctggc cccctttgac tggaactctt
    19261 ttcttcatct tttcgctcta aatgtgtgct gacttttaaa actaaaacgt gtttcttgta
    19321 gacaacagat agatggattt gtttcccttt atccatttag gcagccggtg tcttttgatt
    19381 gtagagcaat tttatatgtg tttaatttta tatgtattga ttgcagtcat gctggaatgc
    19441 gtcactctaa gcgcttcagc tttcccacgg tccagtaaga gctcagctgt tcttctgatg
    19501 ggtttttctt tatgatgtga cctgtgctga ttctctcgtc acttcagtac tctttgtctg
    19561 ttctacgtgg ttctggtcta tgccatgatc tagaatctcc tcccttacct attcctagaa
    19621 tttgaggatt gtttcatggt tcccacggtt tttgtgtgtt cctttcctgt aatttaaagg
    19681 tttttttttc atcatgtaga ttctctactt ctgcaagtcc tgatgttttt atccgctgct
    19741 tgattcacac tgcttgtaag cctttccctt gacttttcta gttgggttat tggggttttt
    19801 aaattccatc tttacttctg tccaagtcat ctttaatatt tctctatctt tacggaattc
    19861 cattttaaaa ttctgtatca cctccatcat ttgcatcagc tccatgtttg tggttttcag
    19921 gcaacactgg gttgttattt tctttaagtt tattgagctg catctctgtg tcttctttac
    19981 acccactgaa ttccttgatg gagtttgtgg ttgttctttt taaattctgt gtcttgagat
    20041 tcatctggta attgtgagga tgatttctag gactggtaga atttgttggg agacactgcc
    20101 ttgctctttt actttgtttg catgtttaca atgagatcta gacatgagga cctcttctgc
    20161 tctttatgtg tccgataagg acagagctgg ttggagcagg acacaggatg tgaaggatag
    20221 tttgagccta aaagttagat atatcttagt tcgaaggaag tcatatggac atgggtctgc
    20281 attggtgtgt aggatatgca tctagatgca acctgaagtc tgtgacatta ttttctatga
    20341 attgtctaac ttaaattatg tgatcattga cttactcagt tcccaggtta taggcatttt
    20401 tattttttgt aattgacttt taatttg
//