LOCUS       114162#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20939 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 114162
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000164598
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8543..8650
                     /db_xref="ENSEMBL:ENSMUSE00001290657"
                     /rank="5"
                     /note="ENSMUSE00001290657"
     exon            8038..8189
                     /db_xref="ENSEMBL:ENSMUSE00000120482"
                     /rank="4"
                     /note="ENSMUSE00000120482"
     exon            7687..7849
                     /db_xref="ENSEMBL:ENSMUSE00001270949"
                     /rank="3"
                     /note="ENSMUSE00001270949"
     exon            6909..7142
                     /db_xref="ENSEMBL:ENSMUSE00001254082"
                     /rank="2"
                     /note="ENSMUSE00001254082"
     exon            4491..5022
                     /db_xref="ENSEMBL:ENSMUSE00000386531"
                     /rank="1"
                     /note="ENSMUSE00000386531"
     misc_feature    4275..9812
                     /note="5 arm"
     misc_feature    9840..9864
                     /note="B1 Gateway"
     misc_feature    9877..9924
                     /note="Frt"
     misc_feature    9925..10424
                     /note="mouse En2 intron"
     primer          complement(9993..10014)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9889..9912)
                     /note="FRT3"
     primer          14090..14108
                     /note="seq primer NF"
     misc_feature    14150..14157
                     /note="RE sites"
     misc_feature    15950..15983
                     /note="loxP"
     misc_feature    complement(14158..14189)
                     /note="Rox"
     misc_feature    complement(15910..15941)
                     /note="Rox"
     misc_feature    14190..14211
                     /note="polylinker"
     CDS             14770..15366
                     /note="puromycin N-acetyl transferase"
     misc_feature    14885..14890
                     /note="Mutated RsrII site"
     intron          10425..10799
                     /note="mouse En2 intron"
     misc_feature    10797..10799
                     /note="Splice Acceptor"
     exon            10800..10853
                     /note="mouse En2 exon"
     primer          10700..10721
                     /note="seq primer FCHK2"
     misc_feature    10854..10976
                     /note="1Dleader"
     misc_feature    10977..11028
                     /note="F2A"
     misc_feature    11124..11840
                     /note="EGFP"
     misc_feature    11034..11123
                     /note="3X NLS"
     misc_feature    13906..14144
                     /note="SV40 polyadenylation site"
     misc_feature    12939..13883
                     /note="ERT2"
     promoter        14222..14733
                     /note="PGK promoter"
     misc_feature    15477..15909
                     /note="PGK pA"
     primer          13964..13987
                     /note="LRPCR RAF5"
     misc_feature    11850..11903
                     /note="T2A"
     misc_feature    11900..11900
                     /note="A to G to kill RsRII site"
     misc_feature    11904..12929
                     /note="Cre"
     misc_feature    10815..10854
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    10815..10830
                     /note="FCHK QC prefix region"
     misc_feature    10831..10848
                     /note="FCHK QC critical region"
     misc_feature    10849..10854
                     /note="FCHK QC suffix region"
     misc_feature    16006..16026
                     /note="B2 Gateway"
     misc_feature    9813..16056
                     /note="Synthetic Cassette"
     exon            17090..17205
                     /db_xref="ENSEMBL:ENSMUSE00001261861"
                     /type="targeted"
                     /rank="7"
                     /note="target exon 1 ENSMUSE00001261861"
     exon            16526..16645
                     /db_xref="ENSEMBL:ENSMUSE00000120488"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 2 ENSMUSE00000120488"
     misc_feature    16057..17410
                     /note="Critical Region"
     misc_feature    17434..17467
                     /note="Downstream LoxP"
     misc_feature    17411..17490
                     /note="synthetic loxP region"
     exon            20083..20245
                     /db_xref="ENSEMBL:ENSMUSE00001249162"
                     /rank="9"
                     /note="ENSMUSE00001249162"
     exon            18473..18645
                     /db_xref="ENSEMBL:ENSMUSE00001279092"
                     /rank="8"
                     /note="ENSMUSE00001279092"
     misc_feature    17491..20939
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaccagag aagaaaacac tggaatcatg aagatttgga agtactgtaa
     4321 gaggcctgtg acttctctcc caacattgta caaatgagta gtgtacatat ggagtaagag
     4381 ttcatctttg tgacctgaga cctagttcaa attccaggtg ggtggtttac ttattgcaca
     4441 actttggcac tggttcttca ctgacaccat gaagaattca tcctacctcc cagggcaaag
     4501 gctggataca aaggaaggtc ccagtgtgcc tcacgtgtgg agaccaagtt ctcaggatcc
     4561 ttcataacat actgcactcc tcaggagcct gggctggggg tgagaggctg ggggctgtgg
     4621 ggacactggt gagaagcacg gcacagacaa gaccaccgtc agagcagaag caggggagag
     4681 ggatactcct ttcagctcac tgtgatgtga ctagcttgcc tccttggtgg caggacgggt
     4741 ccagaatttc acagtggaaa caccacctgg acacaggaca cacccctcag agggatcctt
     4801 taagcctgga agctgagtca tgccagagtt gcctggcacc tgatacaata tacacccttt
     4861 gcccaacgac actggcagga aacagcaggg cctcatgcaa tactctggca gagtctgtaa
     4921 ataccagcac tttgaggagg agaaaggagt gtggacaaga gtctgaagat tgggcctata
     4981 gggaagagcc agcccagcct acagcgctgt cccaggacag aggtgagtca agagtttagt
     5041 cccaggccag ccagagaact gacagaacat accaaaggta atggcagtaa agtggcgggc
     5101 atccttaatg gctctcagtt ctgcccttgg ttctcttgtt gtttacagtt gtttacaggg
     5161 ggaaactggt gaccacaata acaaaaatgt aaggagttac ttgccccacc tgttaaaagg
     5221 ttaagagaga ggggggatta gcaatgtggg ctgagggctt gggtgttgaa ggaggtgcaa
     5281 ggaacctcga tgagggctgg cattgagtaa gacagaggaa gtcatggtga catctgagga
     5341 aagggtgtcc aggcagaggc agagtcctgg caaaggctga ccaggagagt tggggagcag
     5401 ccaggaggca agggcgtctg gcacagagga agccagaggc aagacgagga ggcagctagg
     5461 aagcctcaca ggaaggcagg cagacaaagg gctccttgta tacaccaggc ctcttgtcag
     5521 taccccagac gccctcttca gaagcctgcc aggggctgtg accttacaag ggtaccccct
     5581 tcctcagtcc tgtccttact gtacccttac tgtatcctca ggctcagggg atgaaggaaa
     5641 agagaacagg tgacaggcag cagggaaagt tgacggccag gctctgggac cacggagaca
     5701 gagccacctc cctagcactg agtgctggga acaaagtgca tgaggggtgg ccagttctca
     5761 caggaatgct gatgtcggga gggttgagct actgtgaggt ctctgcaagc tggagctcac
     5821 taggaacact ggggagttga gcctcagccc gaagaaacaa gaggggcaga agtcgggagg
     5881 aaagcatgca ggctagctgg tgggaagcaa gtttgtgggc gtgctaggac tcctttctac
     5941 aagctaactc ccttctgcac agttacaatg acaagcaaga aaagaaagat gagacactga
     6001 gccaggtaag aggggaaggt ggcagagtgg tggccaagga gatggcaagg aacagatagg
     6061 gctaagggga ctcaaagaag gtggatggat gagtctagtt gttcattcac tgaggggagt
     6121 ggggacttct aaaaggccaa gggtttgttg tgaaggaagc aggccacatc ttggctatgg
     6181 gggacacagg agtccagaga gaaagcactt cacaggaaga gagcaagctt tctcctcctc
     6241 ttccttcttc ctacaggctt cctctccttc attctcttct cctacactat gggaattaaa
     6301 cccagggcct tttgtagact aggcaagcac tctccaggta tctactactg ttttgaaaca
     6361 cagtctcact gaattaccca gcctgtctgg aactagttct gtactccagg tagatcttat
     6421 gatcctccta cctctgcctg ccaagtatat gggatgacag acctatacca tcaagcctca
     6481 tgccacagtg ttcaaataga tatcacagga attgagaagt aaatgaggtg tgtgggtccc
     6541 tcctagtgag ccagaggcag tgaacctccc aggaaggtat ggtgtgagca agctatctgc
     6601 tttgtgctgg actccagcca cctctagtat aagtcactcg ctcaacctgt tctcttccac
     6661 ataaccccca ctgacttcat gaagattgtc ccagatgtgc tacaaactac agaggatggg
     6721 aaagctctgt ggtcaaaccg tctggacctt ggctactgca gacagaacaa tattgattgc
     6781 attcacttgt gagcagtatg tggaccacag ccagtagcca tgaagtcggg gtgggagtgt
     6841 gttgtcattc agctagccga gaaaggccct ggctgacccc agccctaaac aatgcctttt
     6901 gcacacaggt tcttggtacc tcccggtgct cagagcagac cctaaaggaa gccaagtcct
     6961 tcctgagaca ggcagatcca ggatggggaa cccaggggat cgagtgtcac tgggggagac
     7021 ctggagcagg gaagtgcacc ctgacataga cagtgaaagg cactccccgt ccttcagtgt
     7081 ggagcggctc accaacatcc ttgatggagg catcccaaac actgagctgc ggagaagagt
     7141 tggtaagcag agtgggaggc caagagccgg cgttgaacct atttgcttct ctagaacagt
     7201 ctggtaacta attctgcaat cctttaacta agaatccttt ctgttgttgt tgctcgttgt
     7261 tttgagacag ggcctcatgt agcccaggct agcctcagac tgcgatgtag ccaaggatga
     7321 cctagaactt ccaattctcc tgcctccatc tctcaagtgc tgggattaaa gatgcaggcc
     7381 accatgccac gatcctatgg tgcatgagaa ccagtgttgt ggattaaacc cagagattta
     7441 tgaattctag tcagcccttc taccgagttc tatctataac taatccttga caccacacac
     7501 ctgtcacgct ctgcaggagt ggacaaagca catttctctc cttgtaggtc tcaattgttt
     7561 catctgttaa atgacagtga cagctggctt cctttccaac accatgaggc gagtgtgtgg
     7621 gaaaatttga tgttgtcagg aaccttatcc aagcccacca aacccctctg ttctgttctg
     7681 ctgcagaaag cctcatccaa cgtgacccag tgtttaattt gaagcatctt tacttcatga
     7741 cccgagatga gctatatgag gatgcagttc aaaagagatt ccatcttgag aagctagcct
     7801 ggagcctggg ctggtcagaa gacggtcctg aacgcattta tgctgacagg tgctcatcca
     7861 gggcagcctc atcagaaagg cttgcctgca acccatgacc ccaaacaaac agtaaagaag
     7921 gacttactgg aacactaggg tgcctgatgc ctcttccccc ctgagcccta cccaggttgt
     7981 aagtttagtg acctgtcttc cacaggctag tcatctacag cgtctcttcc cccacagagt
     8041 ccttgctgga tataacaact taaacttaca tggtattgcc atgaatgcta tcagaagcct
     8101 gggctcagat gagcagattg ctaaatgggg ccaactcggc aaaaacttcc aaatcatcac
     8161 aacatatgcc cagacagagc tgggacatgg taagccaaag ctgctgcatg cagaggtact
     8221 gtacaactct ggaaggccta ttcaagttca tagatgtgca gactaattcg aaaactccca
     8281 gcagatggca gtcaagggtc ctgaggaagt agtgcctttt cctaatgtca catgcactgt
     8341 atgagttacg gggggtaggg ggggagtcat ttcttttgga agcatcagaa tatgtcctca
     8401 cacagcccac agataaaagg gagatacgtc tgtgagtaca gtcaggagct gaccttatcg
     8461 agccccaggc ccacccagaa gctactcaca agtgagtacc caaccttggt ccagtagtct
     8521 aactctcctc cttgatccca agggacatac ctgcagggcc tggagaccga agcaacctat
     8581 gatgcaacca cccaggagtt tgtgatacac agccctacaa tgacttccat caagtggtgg
     8641 cctggggact gtgagtatcc tccttcctgc ctccctgtct ctccacaaag aaaacaaagc
     8701 cggagctgag tagcactaac tttgatgcct aacagacatc aggttgattt cctgttctag
     8761 gctactcact ttgagatcaa ctgaaataga taagatgcta agaatttccg tagttagtga
     8821 ctggaaggtc ctaagggagt tttgagtaga ttaggaaatg cacccaacct gtgggcaatg
     8881 aggttaagaa caagccacat cactgcccac agacaacaca tgtgtaaatg cacacatcac
     8941 aaacaatacc cccacataca gatatgcaag cattcaacac acagaaatac acactgccct
     9001 ccagagtcac agatatacac atcaggagct gttcccatag acacacgtgc attatacaac
     9061 atacacacac tgctctatga cacacatagg caatatgtac acactgccca tacatagaca
     9121 taattgcccc cccacaaata tgcatgtact caacacacac attgttgtca tacagacaca
     9181 tctgtgcata tacatacaac acatacaacc tcccaaacac acatatgcat gtacacagct
     9241 cacactatcc ccatacagac acacctagac ttgtacacat gcattgcctc catgcagaaa
     9301 catttacaca aaaatatgca cactgccctg tcataggcac ttgtacatgt ataaagtaca
     9361 tacactgtcc acccagacac acttgtgtac atatatacac acaatacccc atacacacac
     9421 accacctagt tgtgtcccaa aggcttccaa caacatctag cctccagagt taacacagta
     9481 ggattgccac ctactggagc agagcacatg ctctgtgact agttactaga aatcctccct
     9541 gccactccac acccagacaa gctccaatca cgtggatgga tacaaggtgg cagccatctc
     9601 tgagctctaa gacaggatcc tgccaagagc ccctttgaga catcatttct aaggactgca
     9661 ctgtagcatt tggagaccca gtgacaaaac agctggtgtg gaaactgact aaatatgtgc
     9721 attcaatgaa agcagggcat gggacagaac gggaaaagca aggcaggaag aggagaagga
     9781 caggacaagg acaggagagg taagggggac agaaggcgca taacgatacc acgatatcaa
     9841 caagtttgta caaaaaagca ggctggcgcc ggaaccgaag ttcctattcc gaagttccta
     9901 ttctctagaa agtataggaa cttcgaaccc tttcccacac caccctccac acttgcccca
     9961 aacactgcca actatgtagg aggaaggggt tgggactaac agaagaaccc gttgtgggga
    10021 agctgttggg agggtcactt tatgttcttg cccaaggtca gttgggtggc ctgcttctga
    10081 tgaggtggtc ccaaggtctg gggtagaagg tgagagggac aggccaccaa ggtcagcccc
    10141 ccccccctat cccataggag ccaggtccct ctcctggaca ggaagactga aggggagatg
    10201 ccagagactc agtgaagcct ggggtaccct attggagtcc ttcaaggaaa caaacttggc
    10261 ctcaccaggc ctcagccttg gctcctcctg ggaactctac tgcccttggg atccccttgt
    10321 agttgtgggt tacataggaa gggggacggg attccccttg actggctagc ctactctttt
    10381 cttcagtctt ctccatctcc tctcacctgt ctctcgaccc tttccctagg atagacttgg
    10441 aaaaagataa ggggagaaaa caaatgcaaa cgaggccaga aagattttgg ctgggcattc
    10501 cttccgctag cttttattgg gatcccctag tttgtgatag gccttttagc tacatctgcc
    10561 aatccatctc attttcacac acacacacac cactttcctt ctggtcagtg ggcacatgtc
    10621 cagcctcaag tttatatcac cacccccaat gcccaacact tgtatggcct tgggcgggtc
    10681 atcccccccc ccacccccag tatctgcaac ctcaagctag cttgggtgcg ttggttgtgg
    10741 ataagtagct agactccagc aaccagtaac ctctgccctt tctcctccat gacaaccagg
    10801 ctcccaggtc ccgaaaacca aagaagaaga acgcagatcg cagatcgcag atctctagag
    10861 tcaccgagtt gctttaccgg atgaagaggg ccgaaacata ctgtccaagg cccttgctgg
    10921 caatccaccc aactgaagcc agacacaaac agaaaattgt ggcaccggtg aaacagactt
    10981 tgaattttga ccttctcaag ttggcgggag acgtcgagtc caaccccgga ccggtgcatg
    11041 tggaccccaa gaaaaagcgg aaggtggacc ctaaaaagaa acgcaaagtg gatcctaaga
    11101 aaaaaagaaa ggtgcacgtg tccatggtgt ccaagggcga ggaactgttc accggcgtgg
    11161 tgcccatcct ggtggaactg gatggcgacg tgaacggcca caagttcagc gtgtccggcg
    11221 agggcgaagg cgacgccaca tacggaaagc tgaccctgaa gttcatctgc accaccggca
    11281 agctgcccgt gccttggcct accctcgtga ccacactgac ctacggcgtg cagtgcttca
    11341 gcagataccc cgaccatatg aagcagcacg acttcttcaa gagcgccatg cccgagggct
    11401 acgtgcagga aagaaccatc ttctttaagg acgacggcaa ctacaagacc agggccgaag
    11461 tgaagttcga gggcgacacc ctcgtgaaca gaatcgagct gaagggcatc gacttcaaag
    11521 aggacggcaa catcctgggc cacaagctgg agtacaacta caacagccac aacgtgtaca
    11581 tcatggccga caagcagaaa aacggcatca aagtgaactt caagatccgg cacaacatcg
    11641 aggacggctc cgtgcagctg gccgaccact accagcagaa cacccctatc ggcgacggcc
    11701 ctgtgctgct gcctgacaac cactacctga gcacccagag cgccctgagc aaggacccca
    11761 acgagaagag ggaccacatg gtgctgctgg aattcgtgac cgccgctggc atcaccctgg
    11821 gcatggacga gctgtacaag cctgcaggcg agggcagagg aagtcttcta acatgcggtg
    11881 acgtggagga gaatcccggg ccgtccaatt tactgaccgt acaccaaaat ttgcctgcat
    11941 taccggtcga tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc
    12001 gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg
    12061 catggtgcaa gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt
    12121 atcttctata tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc
    12181 agctaaacat gcttcatcgt cggtctgggc tgccacgacc aagtgacagc aatgctgttt
    12241 cactggttat gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg
    12301 ctctagcgtt cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgacc
    12361 gctgccagga tataaggaat ctggcatttc tggggattgc ttataacacc ctgttacgta
    12421 tagccgaaat tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt
    12481 taatccatat tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta
    12541 gcctgggggt aactaaactg gtcgagcgat ggatttccgt ctctggtgta gctgatgatc
    12601 cgaataacta cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca
    12661 gccagctatc aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg
    12721 gcgctaagga tgactctggt cagagatacc tggcctggtc tggacacagt gcccgtgtcg
    12781 gagccgcgcg agatatggcc cgcgctggag tttcaatacc ggagatcatg caagctggtg
    12841 gctggaccaa tgtaaatatt gtcatgaact atatccgtaa cctggatagt gaaacagggg
    12901 caatggtgcg cctgctggaa gatggcgatc tcgagccatc tgctggagac atgagagctg
    12961 ccaacctttg gccaagcccg ctcatgatca aacgctctaa gaagaacagc ctggccttgt
    13021 ccctgacggc cgaccagatg gtcagtgcct tgttggatgc tgagcccccc atactctatt
    13081 ccgagtatga tcctaccaga cccttcagtg aagcttcgat gatgggctta ctgaccaacc
    13141 tggcagacag ggagctggtt cacatgatca actgggcgaa gagggtgcca ggctttgtgg
    13201 atttgaccct ccatgatcag gtccaccttc tagaatgtgc ctggctagag atcctgatga
    13261 ttggtctcgt ctggcgctcc atggagcacc cagtgaagct actgtttgct cctaacttgc
    13321 tcttggacag gaaccaggga aaatgtgtag agggcatggt ggagatcttc gacatgctgc
    13381 tggctacatc atctcggttc cgcatgatga atctgcaggg agaggagttt gtgtgcctca
    13441 aatctattat tttgcttaat tctggagtgt acacatttct gtccagcacc ctgaagtctc
    13501 tggaagagaa ggaccatatc caccgagtcc tggacaagat cacagacact ttgatccacc
    13561 tgatggccaa ggcaggcctg accctgcagc agcagcacca gcggctggcc cagctcctcc
    13621 tcatcctctc ccacatcagg cacatgagta acaaaggcat ggagcatctg tacagcatga
    13681 agtgcaagaa cgtggtgccc ctctatgacc tgctgctgga ggcggcggac gcccaccgcc
    13741 tacatgcgcc cactagccgt ggaggggcat ccgtggagga gacggaccaa agccacttgg
    13801 ccactgcggg ctctacttca tcgcattcct tgcaaaagta ttacatcacg ggggaggcag
    13861 agggtttccc tgccacagct tgatgaagat ctgagctctt aattaatgat cataatcagc
    13921 catatcacat ctgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
    13981 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
    14041 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    14101 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg tttaaactaa
    14161 ctttaaataa ttggcattat ttaaagttac atatggcggc cgccctaagg ctcgacctcg
    14221 aaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
    14281 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
    14341 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
    14401 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
    14461 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
    14521 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
    14581 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
    14641 cgaaggtctt ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
    14701 ttctcctctt cctcatctcc gggcctttcg acctgcatcc atctagatct cgagcagctg
    14761 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
    14821 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
    14881 gatccagatc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
    14941 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
    15001 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
    15061 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
    15121 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
    15181 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
    15241 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
    15301 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
    15361 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat
    15421 gcatcgatga tatcagatcc ccgggatgca gaaattgatg atctattaaa caataaagat
    15481 gtccactaaa atggaagttt ttcctgtcat actttgttaa gaagggtgag aacagagtac
    15541 ctacattttg aatggaagga ttggagctac gggggtgggg gtggggtggg attagataaa
    15601 tgcntgctct ttactgaagg ctctttacta ttgctttatg ataatgtttc atagttggat
    15661 atcataattt aaacaagcaa aaccaaatta agggccagct cattcctccc actcatgatc
    15721 tatagatcta tagatctctc gtgggatcat tgtttttctc ttgattccca ctttgtggtt
    15781 ctaagtactg tggtttccaa atgtgtcagt ttcatagcct gaagaacgag atcagcagcc
    15841 tctgttccac atacacttca ttctcagtat tgttttgcca agttctaatt ccatcagaag
    15901 ctggtcgagt aactttaaat aattggcatt atttaaagtt aggcgcgcca taacttcgta
    15961 tagcatacat tatacgaagt tatgtcgaga tatttaaata ttagacccag ctttcttgta
    16021 caaagtggtt gatatctcta tagtcgcagt aggcggagat gagtgagcag ccaatatcaa
    16081 atttccaggg gaaagaaacc catctttagg gtcaggctga tgtggttaaa ttccagttct
    16141 gcccctccag accttcagtg tgtcactctg tactctgagc ctcagtgtcc ttatgtggaa
    16201 aataagactc ctcttacttc agccaagagc taagctctag ctgggttctg ataataaatg
    16261 gttgcactca tgatggggcc gacagcagag tgatccatta gctgttgtgg tcaggcacaa
    16321 aaagtcttta aaagtttact cacttcaaac tccttaccaa gccagttact tttgctctag
    16381 ccaggatctt tgtgacgaat ttttctgtca tcaatgactt tccaaggacc caaaggcatc
    16441 tatcaacaac ctaaacccag cagacaggag caagacatga tacataaggg cttccagtga
    16501 taggactctc ttctttctgc ctcagtggga cggacagtca cccatgctgt ggtcctagcc
    16561 catttgatct gcttaggagc ccggcatggc atgcatgcct tcattgtgcc catccggagc
    16621 ctagaggacc acaccccact gccaggtaat ccccattgct tcctcaagga cccatccacc
    16681 cacctagaag atagtcctga atctccaaga gacgcagaga agcttggata cgtctccctt
    16741 gtctagaagg gatctagttg acagagctaa tgtgatctgg atgttagact ggtataagat
    16801 tcagcatctg tctgacttca gcttgcttac tgatgcctga gtcacagtat gtgtgtgtgt
    16861 gtgtgtgtgt ctgtctatct gtctgtctgt gtctgtgtct gtgtctgtgt ctgtgtctgt
    16921 gtatgtgtgt gtctgtgggt gggtgtgtcc ctgtgtgtgt gtctgtctgt ctgtctgtct
    16981 gtctgtctgt gtctgtctat gtgtctgtgt gtgtctgtgt gcatgcatac acacctgcca
    17041 tactgcatgc actcaactga cagacattct catctctacc ctcttctagg aataacagtt
    17101 ggggacatag gccccaagat gggcttcgaa aacatagaca atggcttcct gcgactgaac
    17161 cacgtgaggg ttcccagaga gaacatgctc agtcgctttg cagaggttag atacctggga
    17221 gggctatgga cccagactgc ctagaaaggg gactttccct ttacacagct gccacaggct
    17281 ctgggtctgc ctcagcaaag ctgagtttgg accacaatcc cacttctccc aaaagagttt
    17341 caggggtgcc acaggtcaag ataaaactga gcttaggagc acaatggtgt ctgggaaatc
    17401 ttgccactct gagatggcgc aacgcaatta atgataactt cgtatagcat acattatacg
    17461 aagttatggt ctgagctcgc catcagttca aggaaatact tagcacatgg tttcagagta
    17521 gtcataaggt acagtgacaa agcaatcccc agaaagggaa ggagctgagg gcgaatgtgt
    17581 tgctttcctg aaatcaatta gagagcagat gtgccccaga tgctgacaga agtcctaatt
    17641 agctttttta tggggatagc gtgaagacaa gatgtagctg cgagtgtctc ctggtttcaa
    17701 aggcagatcc atcaggattc gccaccagag gacttagatt taataaggct tacaagttag
    17761 gattttagtc gctgcgctca gtgattgagt ttccgttgtt tttgaactaa gtgtgcattg
    17821 cgttttcctt caaacggtgg ctcatctggg ttcaagaaag gaaacccatt cagatggttc
    17881 tcatggacca cataaactcc actcagcatc ttgccaagtc cagatgttta ctcaaaatct
    17941 acatccaaga caattccaga gaagcaacct acagatgctc caatctattc tcattgatac
    18001 ttctactgga cctgctcctt cctatccaca gatggttcca cagaccctgg acagcaggaa
    18061 aacagccaac tctcctaaga ctggaccaat tccccatccc cattttctta ggtttcccag
    18121 agacatgtcg tcccaaagat catgatgact caaaaagaaa gaaagagaga gagagagaga
    18181 gagagagaga gagagagaga gagaggaagg aaggaaggaa ggaaggaagg aaggaaggaa
    18241 ggaaggaagg aagaaaaaaa gaaaagaaag aagtcctaat tagccagctt tacctcttta
    18301 ggtgtaaaag gtggggattc tcatgcaagc atttttatgt gtcagtgatc atgctgcacc
    18361 aggggtctgc ttcagatgag gaaaagtggg atgcctgcag agacagaagc tcatggaggt
    18421 acagcctggg gtaacttggc tccaacccag catctgtctt tgttgcacac aggtcttgcc
    18481 agatggcacc taccagaggc ttgggacacc acagagcaat tatcttggca tgttggtgac
    18541 acgggtgcag ctgctgtata aaggattcct acccaccctc cagaaggctt gcaccattgc
    18601 tgtgcgctac gcagtaatcc gccaccagtc tcgtcttcgg cctaggcaag cggcacccaa
    18661 aggctgaccc tggcagacat agctaacact cagtcccaca ctgacaccag ctctcccaga
    18721 gccatggtgt tggggcaggt cacacacaga ttcacacgca cacacacaca tgtatgcagt
    18781 tcagatggga cctcaggctc cctctactcc actgacagac tgtgtggtct aacctgttcc
    18841 ttatcagtgt gtggccctat caggctctgt gtgaccatac cttccaccct gcaccatctg
    18901 ctcagactgt ttgctgagtt ccagtttgtt ctacatgact atggctagct agagtgtttg
    18961 tgtctgccag ttgtcaaggc tggttggttg tggtcaggtt gtgtgtggta aaagccaacg
    19021 gcctccaact gcatgaggct ggattcatgc tgcttggcta tgagaagctt ggtctagacc
    19081 ctttcttgtc agttatcggc tcctgttccc tccattccat ggcacagaat ggcaaggact
    19141 gctctatgac acccaattgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgttc
    19201 tttccagaaa gaatacacta gttacattta aaattcatgg aaacaacagt ctctggggat
    19261 aggtcccaaa tggacacact ctctagaagc agcaaaccat ggtgagaaca agaagtgggg
    19321 atacagcaca tcacagccta acaattcatg gtgctcagga gagtttttat cccagccctg
    19381 ctgactggcc ctgggcttga taaggctctc cctggtctat gtcctgcctg tatatagtga
    19441 ggtcaccagg actgagcggc cctaggacct ggcgttattt gatgccatgg gccccagaca
    19501 tggcaccagc ctgccataaa catactgagg attagatgac atgcagccac gggcatacgc
    19561 ccttctttct tcctcagctt ctatgtccgt aaaatagagg tgagacaggc agagtttcag
    19621 aggtcaaata tgataaggct acaaagcttg aattccagac gcccttactc tgtccgggga
    19681 gcagagggat ctcaccctga ttatgtcaca gagatggaca tagaagactt ccttttttat
    19741 gtgtctttgg acccaagttc atagcctaag ggttgttaag ctgaatgcat ttcctataac
    19801 accgcaggca catggagggt catgagtcat cagtggggca ggaccacacc ccttcatctg
    19861 gtctttgggt ccagatggtt tggagttcag aacctttcag actttcagct aagataatgg
    19921 ttgtatatgc tgtggacatt tatacagaaa aatacgtatg gatattggtg gtaggtgagg
    19981 tttaaaacac agtagcctca tttcagcctt tcagtggtcc atttgtatgg cttgacagtg
    20041 tcaaggtgaa ctcctaaacc tgtgcttctt ttctctgctc agtgacccag aggcaaaaat
    20101 cctggaatac cagacacagc agcagaaact cctgcctcag cttgctgtga gctatgcctt
    20161 gcatttcatg accaccagcc tcttacaatt cttccacagc tcctacagtg atattctgaa
    20221 gagagacttc agcctcctgc ctgaggtaca ctctgcttcc tgggaactgc ttatagaggg
    20281 tggcaaatgc tgttccagtt gtggggaaat tactgaagct gggctcccat agaccctcag
    20341 tctcccaatg tccaaggcat cagagaaaat ggaccataat atggagatcc actggcccct
    20401 gacccctaga gagttccaac aatgttagag gtaatgcaag ggtggaggac acagatacac
    20461 aatcccaggt atatgatgga aacccaaaca actccaaagt catttgaaca catttgatgg
    20521 catactttcg cctgcattaa tctgatttat cctactggca acaaatataa cagaaccatc
    20581 aatgtgttca actgccaaga cttcccagag tccttcacca tagtatgcac acttcacagg
    20641 ctctgctttg cctttgaaaa atccaaaaat atatcagacc tagagagagc actgtgttcc
    20701 aagctgtgca tggattcaat attctcagtc tttggtgagg ctaagactca caaacgcctt
    20761 cagtctgtgt aggtgctatg actttaaagc attgcttcat taagtctgtc catggtatcc
    20821 ataatttaca ttctttattt gcaagtaagg aaactagaat agaaagaaaa aggaggcctt
    20881 tctcccaagg gtgtaaaagc ctgaaagatg gatagaggtt ttcaagaacc aaaatctta
//