LOCUS       297897#pL1L2_GTK_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21679 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GTK_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 297897
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000035372
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            6904..6950
                     /db_xref="ENSEMBL:ENSMUSE00000276666"
                     /rank="1"
                     /note="ENSMUSE00000276666"
     misc_feature    4275..9711
                     /note="5 arm"
     misc_feature    9739..9763
                     /note="B1 Gateway"
     misc_feature    9776..9823
                     /note="Frt"
     misc_feature    9824..10324
                     /note="mouse En2 intron"
     primer          complement(9892..9913)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9788..9811)
                     /note="FRT3"
     primer          13827..13845
                     /note="seq primer NF"
     misc_feature    13887..13894
                     /note="RE sites"
     misc_feature    15687..15720
                     /note="loxP"
     misc_feature    complement(13895..13926)
                     /note="Rox"
     misc_feature    complement(15647..15678)
                     /note="Rox"
     misc_feature    13927..13948
                     /note="polylinker"
     CDS             14507..15103
                     /note="puromycin N-acetyl transferase"
     misc_feature    14622..14627
                     /note="Mutated RsrII site"
     misc_feature    10861..11577
                     /note="EGFP"
     misc_feature    10771..10860
                     /note="3X NLS"
     misc_feature    13643..13881
                     /note="SV40 polyadenylation site"
     misc_feature    12676..13620
                     /note="ERT2"
     promoter        13959..14470
                     /note="PGK promoter"
     misc_feature    15214..15646
                     /note="PGK pA"
     primer          13701..13724
                     /note="LRPCR RAF5"
     misc_feature    11587..11640
                     /note="T2A"
     misc_feature    11637..11637
                     /note="A to G to kill RsRII site"
     misc_feature    11641..12666
                     /note="Cre"
     misc_feature    10599..10620
                     /note="seq primer FCHK2"
     misc_feature    10325..10761
                     /note="mouse En2 exon"
     misc_feature    10713..10781
                     /note="FCHK cre_ert_gtk"
     misc_feature    10713..10748
                     /note="FCHK QC prefix region"
     misc_feature    10749..10761
                     /note="FCHK QC critical region"
     misc_feature    10762..10781
                     /note="FCHK QC suffix region"
     misc_feature    15743..15763
                     /note="B2 Gateway"
     misc_feature    9712..15793
                     /note="Synthetic Cassette"
     exon            16570..17228
                     /db_xref="ENSEMBL:ENSMUSE00000276657"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000276657"
     misc_feature    15794..17365
                     /note="Critical Region"
     misc_feature    17389..17422
                     /note="Downstream LoxP"
     misc_feature    17366..17445
                     /note="synthetic loxP region"
     misc_feature    17446..21679
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatgcttg tttagagtgc atgaagccgc aggtctgatc cttagagtag
     4321 aaatcagatg tggtagcgtg tggttgtgat ctcagcactt ggtatatcga gaaggaccaa
     4381 aagttcaggg ctatcctcag ctactggtca agtttcccca tctcaaaaac aaaaagaagg
     4441 gctggtgaga tggccctgtg ctaagagtgc tggctgcact tggagaggat ctgagtatac
     4501 ttcctagaac ccacatggcg gctcaaaaca ccctgtaact ccggttccag gggctccagt
     4561 gccctattct gtcctccaca ggttatatga tgcacagaga tgtatgcagg cagaacacca
     4621 tgcacacaca cacacaatct ttaaaaaagc acaaaaggga aaaaaagttt tgaaatatct
     4681 ttgattgtat gttcttcttt aaaaatactg tcttgtgaaa caaaagaaaa attaacattt
     4741 aaaataatat aattttagtt tcacgtggga cagaagcaaa gagaaaagtc atagttctct
     4801 ctgtgcaacc cagaaggggt gttcttcagg ctttttttta aaaatttatt tatttagggg
     4861 gttttttgag acaggatttt tctatgtagc cctggctgtc ctggaactca ctctgtagac
     4921 cagactggcc ccgaactcag aaatccacct gcctttgtct ccgaagtgct ggggttaaag
     4981 gcgccaccac tgcccagctc ttcaggcctc ctaaagaaag caagctccgt cttgcttctg
     5041 tagttaaact aatcatcttc tgcctggacc agaagagaca ttgtattccg tcaaggaaac
     5101 tcacgagcta tgggtagtgt agctacagct gctgcaaagg cagcactctt ccctcttgtg
     5161 ggtgctggga gctgaacttg ggtcttctgc aagagcaaca agagctctaa ttgctgagcc
     5221 atctcacgag ctcatgctag atagtttgga tgtcaactta gcacaatcta gaatccgctg
     5281 caaagaagag aatctcagtt gagaaaatcc ctctgtaaga ttcggctcta aggcattttc
     5341 ttaatttgtg attgatgggt gatcatcctg gactctctaa gaagcaggct gagcaagcca
     5401 gtaaacagca attctctatg gcctttgtat cagctcctac ctccaggttt gagtccctgt
     5461 cctaacttcc tttggtgatg aacaatgata tggaagtgta agccaaataa acccttcttc
     5521 cccagtgtgc ttttggtcgt ggtgtttcat tacaatagca atcctaagat acaaccccaa
     5581 tacataacat ttattttttt attgagactg tgtagccgtg gctgacctgg aattcactaa
     5641 gtagagcagg ctgacctcaa attcatggaa atccacctgc ctctgctgag tactgggatt
     5701 aagggtgtgt accaccatgc ccagcccaac agaacatttg aaaacatttg atattgccgt
     5761 taactcatgt tctgcaattg aaaggtaatg tcatatttag ttctctgcat attccacgag
     5821 ttgtgctgac attctgagtc ttgggctggg gatgtggctc agttagttag tagagcactt
     5881 gcctagcatg caagaagccc tgggtttgat cctcagcgcc tcacagaact gggaatgaca
     5941 acacactcct ggaaccctaa cactcgggaa gcattggcaa gaggatcaga agttttgagt
     6001 tatcctcagc tgtttattga gtgtgagggc agtctgggct acatgagagc ttatgatgag
     6061 acttccagct ttgcacatag atttggaacc aaatttagac tctatcctcc tctagattaa
     6121 aaatttgagc taagtcttct tttacatcaa tcatatatag agacagagac acacacacac
     6181 acacacacac acacacacac acacacacac acacacacac acatatatat atatatatat
     6241 atatatatat atatatatat atatatatat atatatagag agagagagag agagagagag
     6301 agagagagag agagagagaa acactaaatc agaaggctgg atcgatggct cagcggttaa
     6361 gagcactgac tgcacttcca gaggtcctga gttcaaatcc caccaaccac atggtggccc
     6421 acaaccatct gtaataggat ctgatgccct cttctggtgt gtctgaagac agcaacagtg
     6481 tactcatata cattagataa ataaataaat catttttaaa aataaataaa agcgccagct
     6541 ctccaggggc tgctgcactg cactacccaa gagactccac tctgcaaaga tcacgtgggc
     6601 cttctcggta ctgctcttac tctctaaaag aaattgacca acatttgcat attatgagac
     6661 tactgtattt ctgtaaataa ttttatataa aaccatataa accggatgaa tgggagtacc
     6721 ctctaaacaa aagaatctgt tccacgtgtc tagtttcata agcaaacctt tcggcttaga
     6781 ttgtcatagc tccatagtag gcagaaaaga aaaaaaaaaa atcagctaaa ccagagccag
     6841 ccagttctgt ttaactgtaa gctactttgc atattttcac taaatgacca cctataaatc
     6901 aggttcagtc agactcagcc ccaaatacag catcttgaac tacaaaacag gtaagagaca
     6961 ctgctccaca atgacccacg gggagacgat gattcctaag aaaggctcca ggcacagtga
     7021 aaaaaatttg tcatcgatag gattatgtta ttgtcttaag aaattgatga ctgcccctac
     7081 aatgcaaggc tcagattgtc cccctgatga aatctgatga acttagcttc tggcatctgg
     7141 tgccagcctt gctgctgact ttgaaagctt agcaatttac catagtctct gaggagggag
     7201 ggaaggaggt agctccaagc tccccccagc tctcccatcc cataattcaa ttgtatacac
     7261 aatggtaggt ttcttttatt ttttaaattc tagatttttc tttaattatg aaagttgacg
     7321 tttttatgct cagtggagcg tcgagaatct cttaggattt catcattaag acttcattgt
     7381 ctatttccca gaagagctct cagacagaaa cctgagatgg tcccgaggta ggtgtgcgtg
     7441 tgagccatgc gtgcatgtgg aagcctggaa tgacagcata agaaatccac acacacaccc
     7501 agaccccact gggctcccgt cttcttgctc ttgtgttttc attttcctaa gttgcttggt
     7561 taaattcccc tgtgtgcatt aagggatccc acttcaatgc tccctgatgg gtggagtcct
     7621 tgaaaatgca tactagaaaa acctggccat cactgtgtct tcagacatgc catgcttcaa
     7681 gaatgagtca aaatctcatt aaagtgtaga atcttaatta cctgtggtgt aaacagatta
     7741 ctcgggtttc atctgggtca agggttaaga tattatttaa gatatttttc agggtaaaaa
     7801 gcatgactca aaataaaatt ctttttattt ttattttggc tttgacaata actgctttac
     7861 tttggaggaa cctggctgtt ctagtctcct gtctgttgct gtgagaaaat actctgacga
     7921 aaagcaccct gggggcagaa aaagtctgtt tcagcttaca agtgacagtc tgtcatcaag
     7981 gggagcaagg cagaggctca cggagcagga gcttgaagta gagaccatgg aggaacctgg
     8041 tttgctggcg cactttctgg cttgctggct ttcttctgca gcccagggtc acctgcccag
     8101 gaaatgatgc aaggacagtg gggtgggcca ctgtccacaa gacacaagac aataccctgg
     8161 gggctgtaac tccagatcag aggatctgac acagatctct ctctctccct ctctgtgatt
     8221 ttccctctct ccctctctct ccctctccct ctccctctcc ctctcctctc tccctctcct
     8281 ccctctcctc cctctccctc tctccctctc cctctctctc tctgtctctc tgtctctctc
     8341 tctctctctc tctctctctc tctctctctc tctctctctc tctctctctc tcttgatact
     8401 gcaggcgccc tggcagcatg tggatagcag gcctgtcttg ttttgacaca gggtctcctg
     8461 gatcccaggc cagtctagga taactttaag cttctagttt atttggtgct gggattgcac
     8521 ccagggctta gtgcacgcca ggcaaggacc tatcagctga gctgcatcct ctgcccccat
     8581 ttgctggctt tggtggctta ggcatacctg ccctcttgaa ttcctgactt gggaatttcc
     8641 taactagttt cttctctcat taacattttc ctttttgata gaattctggc tcctgtttca
     8701 tcttccagag acatttgtct tgtgttagtt attgtgggtc atttggtcag taatttggac
     8761 tctcccattt cccgttcctc cgttttagtt tttagttggc accatatcaa cttatcctct
     8821 ccagactctt tagcacacat ctttccaggc acttactctg tttccagcca gtgccactgt
     8881 gacttcttac aaccccttcc tccttcaccc aaagtccagt cccccagcac ccctgtggcc
     8941 tcccagcccc tacagccccc acagcccttt ctgattctta tcttttcaga ggccatacgc
     9001 tggccttttc acagatggaa acacactaga atctggtctt aaagcctgtt ctttccctca
     9061 cgtggtctgt agggttcctg ctgtgctcag gtaaagaaga ttcagagcca agttaggtgt
     9121 agtggcacat gcctgctagg ctcctgcctc agctagctaa caaatagtag gttagcaaat
     9181 acagagcaag acctagtctc aaaaaataca aatgtgtgtg tgtgtgagag agagagagag
     9241 agaggcagtg ttgtctaatc ttgcttataa acacttagct tgttcgcttt ggctgcactt
     9301 ctgttttgtt tagtattgcc aagtctgtga ctctgaaaga cactggaaaa ttactccctg
     9361 agaagcagcc cgggagctcc agttttttct atgaagtgca gagaaagagg caccataagt
     9421 gtccatctgt gaagaaaaag ggaaagacct aattccagga cttgatgcat cacttggact
     9481 tttaaaatgt aaactcttta aaccactgca gatagtcaat atctcaagtg ggtataggaa
     9541 gccagcgagg cccagagagg cttaaggaac tggcctgagg acacagagca ggggacagtc
     9601 aggattgagc cctaaagggg ttacaatgaa agcacacaag cactccttcc ctgagtccca
     9661 ggtgataggg taaccgaaac gtggcctgga aaatccactg aagctacttt aaaggcgcat
     9721 aacgatacca cgatatcaac aagtttgtac aaaaaagcag gctggcgccg gaaccgaagt
     9781 tcctattccg aagttcctat tctctagaaa gtataggaac ttcgaaccct ttcccacacc
     9841 accctccaca cttgccccaa acactgccaa ctatgtagga ggaaggggtt gggactaaca
     9901 gaagaacccg ttgtggggaa gctgttggga gggtcacttt atgttcttgc ccaaggtcag
     9961 ttgggtggcc tgcttctgat gaggtggtcc caaggtctgg ggtagaaggt gagagggaca
    10021 ggccaccaag gtcagccccc cccccctatc ccataggagc caggtccctc tcctggacag
    10081 gaagactgaa ggggagatgc cagagactca gtgaagcctg gggtacccta ttggagtcct
    10141 tcaaggaaac aaacttggcc tcaccaggcc tcagccttgg ctcctcctgg gaactctact
    10201 gcccttggga tccccttgta gttgtgggtt acataggaag ggggacggga ttccccttga
    10261 ctggctagcc tactcttttc ttcagtcttc tccatctcct ctcacctgtc tctcgaccct
    10321 ttccctagga tagacttgga aaaagataag gggagaaaac aaatgcaaac gaggccagaa
    10381 agattttggc tgggcattcc ttccgctagc ttttattggg atcccctagt ttgtgatagg
    10441 ccttttagct acatctgcca atccatctca ttttcacaca cacacacacc actttccttc
    10501 tggtcagtgg gcacatgtcc agcctcaagt ttatatcacc acccccaatg cccaacactt
    10561 gtatggcctt gggcgggtca tccccccccc cacccccagt atctgcaacc tcaagctagc
    10621 ttgggtgcgt tggttgtgga taagtagcta gactccagca accagtaacc tctgcccttt
    10681 ctcctccatg acaaccaggt cccaggtccc gaaaaccaaa gaagaagaac gcagatcata
    10741 actgactagg aggccaccat gggcggaccg gtgcatgtgg accccaagaa aaagcggaag
    10801 gtggacccta aaaagaaacg caaagtggat cctaagaaaa aaagaaaggt gcacgtgtcc
    10861 atggtgtcca agggcgagga actgttcacc ggcgtggtgc ccatcctggt ggaactggat
    10921 ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgaaggcga cgccacatac
    10981 ggaaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ttggcctacc
    11041 ctcgtgacca cactgaccta cggcgtgcag tgcttcagca gataccccga ccatatgaag
    11101 cagcacgact tcttcaagag cgccatgccc gagggctacg tgcaggaaag aaccatcttc
    11161 tttaaggacg acggcaacta caagaccagg gccgaagtga agttcgaggg cgacaccctc
    11221 gtgaacagaa tcgagctgaa gggcatcgac ttcaaagagg acggcaacat cctgggccac
    11281 aagctggagt acaactacaa cagccacaac gtgtacatca tggccgacaa gcagaaaaac
    11341 ggcatcaaag tgaacttcaa gatccggcac aacatcgagg acggctccgt gcagctggcc
    11401 gaccactacc agcagaacac ccctatcggc gacggccctg tgctgctgcc tgacaaccac
    11461 tacctgagca cccagagcgc cctgagcaag gaccccaacg agaagaggga ccacatggtg
    11521 ctgctggaat tcgtgaccgc cgctggcatc accctgggca tggacgagct gtacaagcct
    11581 gcaggcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccgggccg
    11641 tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat
    11701 gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc
    11761 tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg
    11821 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc
    11881 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg
    11941 tctgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga
    12001 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat
    12061 ttcgaccagg ttcgttcact catggaaaat agcgaccgct gccaggatat aaggaatctg
    12121 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg
    12181 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa
    12241 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc
    12301 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg
    12361 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg
    12421 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag
    12481 agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc
    12541 gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc
    12601 atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat
    12661 ggcgatctcg agccatctgc tggagacatg agagctgcca acctttggcc aagcccgctc
    12721 atgatcaaac gctctaagaa gaacagcctg gccttgtccc tgacggccga ccagatggtc
    12781 agtgccttgt tggatgctga gccccccata ctctattccg agtatgatcc taccagaccc
    12841 ttcagtgaag cttcgatgat gggcttactg accaacctgg cagacaggga gctggttcac
    12901 atgatcaact gggcgaagag ggtgccaggc tttgtggatt tgaccctcca tgatcaggtc
    12961 caccttctag aatgtgcctg gctagagatc ctgatgattg gtctcgtctg gcgctccatg
    13021 gagcacccag tgaagctact gtttgctcct aacttgctct tggacaggaa ccagggaaaa
    13081 tgtgtagagg gcatggtgga gatcttcgac atgctgctgg ctacatcatc tcggttccgc
    13141 atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat ctattatttt gcttaattct
    13201 ggagtgtaca catttctgtc cagcaccctg aagtctctgg aagagaagga ccatatccac
    13261 cgagtcctgg acaagatcac agacactttg atccacctga tggccaaggc aggcctgacc
    13321 ctgcagcagc agcaccagcg gctggcccag ctcctcctca tcctctccca catcaggcac
    13381 atgagtaaca aaggcatgga gcatctgtac agcatgaagt gcaagaacgt ggtgcccctc
    13441 tatgacctgc tgctggaggc ggcggacgcc caccgcctac atgcgcccac tagccgtgga
    13501 ggggcatccg tggaggagac ggaccaaagc cacttggcca ctgcgggctc tacttcatcg
    13561 cattccttgc aaaagtatta catcacgggg gaggcagagg gtttccctgc cacagcttga
    13621 tgaagatctg agctcttaat taatgatcat aatcagccat atcacatctg tagaggtttt
    13681 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
    13741 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    13801 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    13861 caatgtatct tatcatgtct ggatccgttt aaactaactt taaataattg gcattattta
    13921 aagttacata tggcggccgc cctaaggctc gacctcgaaa ttctaccggg taggggaggc
    13981 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
    14041 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
    14101 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
    14161 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
    14221 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
    14281 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
    14341 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcttccg gaggcccggc
    14401 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
    14461 cctttcgacc tgcatccatc tagatctcga gcagctgaag cttaccatga ccgagtacaa
    14521 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
    14581 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccagatcgcc acatcgagcg
    14641 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
    14701 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
    14761 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
    14821 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
    14881 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
    14941 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
    15001 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
    15061 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
    15121 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgatgatat cagatccccg
    15181 ggatgcagaa attgatgatc tattaaacaa taaagatgtc cactaaaatg gaagtttttc
    15241 ctgtcatact ttgttaagaa gggtgagaac agagtaccta cattttgaat ggaaggattg
    15301 gagctacggg ggtgggggtg gggtgggatt agataaatgc ntgctcttta ctgaaggctc
    15361 tttactattg ctttatgata atgtttcata gttggatatc ataatttaaa caagcaaaac
    15421 caaattaagg gccagctcat tcctcccact catgatctat agatctatag atctctcgtg
    15481 ggatcattgt ttttctcttg attcccactt tgtggttcta agtactgtgg tttccaaatg
    15541 tgtcagtttc atagcctgaa gaacgagatc agcagcctct gttccacata cacttcattc
    15601 tcagtattgt tttgccaagt tctaattcca tcagaagctg gtcgagtaac tttaaataat
    15661 tggcattatt taaagttagg cgcgccataa cttcgtatag catacattat acgaagttat
    15721 gtcgagatat ttaaatatta gacccagctt tcttgtacaa agtggttgat atctctatag
    15781 tcgcagtagg cggcgtgtag ctcgaagtac tgtgctgacc atgtgtaaga ctctgggtct
    15841 agtgcccaca gcaacacaca cacacgcaca cacacacaca cacacacaca cacagacaca
    15901 cacacacaca cacacacaca cacacagaga gagacagaga cagagacaga gacagagaca
    15961 cagagatgta ataacatttt tgatgaagaa aagataaaaa agtcctctcc tttcgctctt
    16021 tctttcttct ttctttcttt ctctttctct ttctttcttt cttccccctc tcttccttct
    16081 ttccttcctt ccttccttct ctctctcttt ctctctctct ttctttctct ctctctcttt
    16141 ctttctttct ttcttccccc ctctctctct cttccttcct tccttccttt ctttctttct
    16201 ttctttcttt ctttctttct ttctttcttt ctttctttct ttctttcttt ctttctttct
    16261 ttccttcttt ctttcttgtt gttagtgtta ctgttttgag acagtttcac tatatagccc
    16321 agactaccct tgaacttact ctgtagacca ggctgcccct gaactcacag ctatcctcct
    16381 gcttcatttt cttgagtgct ggaattacaa gtccccacca ccccagtgcc tctacacaaa
    16441 tgactataac cctgtctact tttctatatg agtcctaagc agaaatctat aagacctgga
    16501 tctcaaagaa agtggatcaa aactgctctt ttaatctggt caaactgata agcactcctg
    16561 tgtttctagg tgaaaggaaa cgatggacac cagaagctac cccagccctc cagacaggct
    16621 ctcggtcttc gctgagtcgg cccacttacc actgtccagg cccttctacc tggaccccat
    16681 ggtcactgtc cacctatgcc ccgagacccc ggtaccggcc tcttacacag atgagctgcc
    16741 tctgctgccc ttctccagcg acaccctgat catgaacaat tacggggatc catacccttt
    16801 tcccttcccc atgccttaca ccaactacag gcgctgtgac tacacctacg ggccggcctt
    16861 catccgcaag aggaacgagc gagagaggca acgagtcaag tgtgtgaacg aaggctacgc
    16921 ccggctgcgg cggcacctgc cggaggacta cctggagaaa cggctcagca aagtggagac
    16981 cctcagagct gccatcaaat atatcagcta cctgcagtct ctcttgtacc cggatgaatc
    17041 tgagaccaag aagaaccctc gaacagccag ctgcggctcc ctggacccgg ctctgagagt
    17101 catttgaatc agttcttcct cgaatgggag tcttctgctg ccgcagccag tacattctca
    17161 tacacatggt ttcgaggaca caacagtacc tcccaatacc aaccttcatt aaatgttgct
    17221 tgcctgggtt tcagatgtac taaagtagct catgtagacc aagagtccca tgctggtctc
    17281 tctgctttaa gaactctgag ggtagagcag tccccaagtt ctctgcacta ccatccaccc
    17341 cgcccttccc acagccttgg ctgaagagat ggcgcaacgc aattaatgat aacttcgtat
    17401 agcatacatt atacgaagtt atggtctgag ctcgccatca gttcatccct tgatttgcaa
    17461 tcagtttagg cacatctttt tcttgcagat agacttccct aagcagttta gcacaccgct
    17521 ttccaagcct ctgggggtgg ctgaggaggt gactcagaga ttaaacactc actcagcagg
    17581 caggcaatct taaggcctgg agttcagatc ctctgcattc cgtgtaaata aacgccaggt
    17641 gggtgtgaca gtctacctgt aaatccagcc tctgaccgtt gagggcgggg gtctccagag
    17701 caggcaggtt accaaggcta gtcatttggg caagctctgg gtttgattga gagaccttaa
    17761 ttcagtgaat aaggtagaag atcgatcaag ggtgattcaa gcctctgtat gcacatgccc
    17821 agcgtgaaca tactccccat gtgtgaccat acatatgcga acatacctat acacacatgg
    17881 gtaccacatg ctcatgaaaa tggagggggc aaaacaaaac aaaacaaaac aaggttctgg
    17941 agtctaattg atcagtttga agtttcactt cctctccatg ttgggttctt cacccacaaa
    18001 atgaagaaaa taatgtcagc aatttcctat gagtttcttc agggaataac tgtgcctgag
    18061 acctaatcag gattcatgac cgttgcttgt tattgtcctt gcccttgtgt agtgcacagc
    18121 atcaccactg tccagtcttg tccatatcag ggcatcttcg gctgctctgt gggaccttcc
    18181 tttactcctg ggaggagcat ctgggccttt gtcttcataa ctaccttcag ccctcctccc
    18241 tcagctccca ggttccaact gtcactaagc ccagtcagga tgttgagact tccctccctc
    18301 tgtctgtcac actgatctct gctgtcatgt atctcgactt cctgtcccag ccattactgc
    18361 tgctagattg tgctcacaag tctgtctttg ggttcctagc ctgctgtctt tacacaccac
    18421 acacacccaa ctctgttttc tgagagatag ctaccagtct gtgaaattct taaagcactg
    18481 tcgctcacac tatttgatga cttctgttgg atgactacct ttaaatgtcc tcacttactt
    18541 tgtggatttt tgttttgttt tgttgctctc attacatagc tctggctgga ttctaactca
    18601 cctgcctgtg cctccagagg cctagtatta aaggcacatg ccacaatgcc tggctccact
    18661 tggctttaaa actccttgta ctctgatatc cccataggag agcacaatct agcaaacaca
    18721 tccttctctc tttactctcc cattccaacc tggaatattt tcccttcttc caccactgac
    18781 atcccccgtt ctcaatcagt aatcctttct ggttggcaga cttcgttagt ctttcttaga
    18841 ttcgtattca aggcacatgt ctggaccaca gtcattctgg taattacaaa actagctttg
    18901 agagcatcat caaggtactc agagtgccag agaaaactca acccaaagtg tcacaaccaa
    18961 tctggaggat gtattcctca tgtaacagaa ggcctgaaca ctccaacaac tggccatttt
    19021 taaatctgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtc tgtctgtctg tctgtctgtc
    19081 tctgtctctt tgaatgtgtg tgtgtctcca ccaagaagtt ttatgtgaac aaaaagaaca
    19141 cagagctgag gagatagctc agtggttaag agcacttgct gctctttcag aggacccagg
    19201 tttggtcccc aggttctaca aattcacaac tgtctataaa tccagttcta gcgggaatgg
    19261 atccaaggtc ctcctctggc ccctgcaggc accaggcctg cctgcagtgc acatccatcc
    19321 acgcaggcag aagagtcata cacatgcaat acaaataaaa gactaacttc ttaaatcagt
    19381 gtaggatgca ctgtgagaga gcagcgggct tcttgagctc caatgcctaa gaccctaagt
    19441 ctgtctgcat aacgttctca ggaaatagct cccagaatct tctagaattg aatgcctcct
    19501 tattcctgca caggaagaag aaagtctgta cacaagattc ttggggcagt cttgagacgc
    19561 attctaaggg agtggctcgg gactactgaa cccgcctgca ggggaaaagg gtgtcctgaa
    19621 tgacactgtg cgcctgggag ctggggtaca tttggccgat gtgcacatag atccatggtt
    19681 caaaacagga actggtggga ggggcaaatg gctgaaggag agactccaaa tggctgaagg
    19741 agagactcca atacgtgttc ctgtgcccag gggcgaatgg ctgaaagaga gacccccata
    19801 catgttcctg tgcctagggg tgaatggcag aaggagagac ccccatacat gtccctgtgc
    19861 attttttggg tcttctctat tgaacacagt gccaggcatt tcttcagggc accacaactg
    19921 tctcaaaata ccagtcttct ctgggtaatg atttatttat ttaatgtgta ttggtatttt
    19981 gtctgcatgc gtgtctatgc aagggtgtca gatcccccgg aactggagtt acagacagtg
    20041 tgagctgtca cgtgagtgct gggaattgaa cccaggtcct ctggaagagc agccagtgct
    20101 cttaatcacg gaatcatctc tccagcccca cagaatctgg actcctattc ctttggtcac
    20161 caggctgtta cttgcatttc tgcagctcac atacttcttc cactccttag gagcatcggt
    20221 tcctgggaga cctttgctgt gtgtgtgtgt gtgtgtgtgt gtatgcatgg acctgaggag
    20281 gttggaaggt gtctttctcc gttgctctca tcatcatcat catcatcatc atcatcatca
    20341 ttattattat tattatagga ttagtggtag aatctctcat tgaacctaga gctctcaatt
    20401 tttagttaac tagtcagtca ttaacttctt tcgatgctag gattataaac tcagccaatt
    20461 ggctgctgct gatccaaata caagcttcat gctttcacag caagcagctt atctatccac
    20521 tgaactatct ttctagcctc ttgccttcac taggtagatg ccaacagccc tcagcccttg
    20581 gagagctcag cccaccctca taggactccc ctagagtccc tggagctaaa gggagtatac
    20641 acaatttgcc tttatttctc caccttaccc acaacttcaa gtttcccgat gcttccggtc
    20701 accctctgca catgctcagt accttcctga gccctgttgt agcctgctgg ctcacaacca
    20761 tagctacctg cttggcaact tgcctggcaa ccaagagttg ttagaccaag gccatgagct
    20821 ccaactgtca ggtgggactg agactggggc aaggctgagg tctagactgg atttgcactt
    20881 ttaggtaagt ccctttggtg ttcgaactgc ggctttgggc aggggtagac cctaacaagc
    20941 tctaggcggc agctgctgcc tctgaagaag tccacaaatg ggatgatcct gagtcctgta
    21001 ggtgaccagg ttaggagggg agttggtggc ttctgctggg ggtcagttgg tcagggcagg
    21061 gtgtgtaaag agaatgagca tgctccaagg agtcccagcc actggggagg aggaaggagg
    21121 taacaccaac gggagccgag ctgccaggta tttccgtggc tcatctttgg agacttaaca
    21181 gtgagcgtgc aggttctgaa ggaggattaa agagggctct ctaagctcct agcttaagtt
    21241 cttcagccct ccctagtccc ccagggacat tctaaggtaa gtattccaaa ggggaaagtc
    21301 tgggtgtgtg tgggaacctt tgggcgtgtc ctctgctggg ttgaggctac agctgtgctg
    21361 cctgtgtttg ctccaggtgg gcgcgctgaa ggtcatgcag ccctctgcca gtcctgggct
    21421 gccactgccc aaatactgca gtgtggccac aaccgtgaaa gcccctgacc tgcatggtgc
    21481 tgtgccgccc tgggacatgt ccttcacctg tccctttgcc acgcaagcac cctggctggc
    21541 cacgcactgc acattcacca ggtaccagtc tctgtccctc gtcctttttt tctcctcttt
    21601 tcctggtcct cctaatggca tttggatgcc ctctattgcc tgggacttta tagaacagca
    21661 tcttcagtca gctctctat
//