LOCUS       115490#pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        23115 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 115490
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000023809
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            4353..4535
                     /db_xref="ENSEMBL:ENSMUSE00000397329"
                     /rank="2"
                     /note="ENSMUSE00000397329"
     exon            4812..5003
                     /db_xref="ENSEMBL:ENSMUSE00000359366"
                     /rank="3"
                     /note="ENSMUSE00000359366"
     exon            5256..5327
                     /db_xref="ENSEMBL:ENSMUSE00000404158"
                     /rank="4"
                     /note="ENSMUSE00000404158"
     exon            5548..5660
                     /db_xref="ENSEMBL:ENSMUSE00000364112"
                     /rank="5"
                     /note="ENSMUSE00000364112"
     exon            5779..6009
                     /db_xref="ENSEMBL:ENSMUSE00000347603"
                     /rank="6"
                     /note="ENSMUSE00000347603"
     misc_feature    4275..10271
                     /note="5 arm"
     misc_feature    10299..10323
                     /note="B1 Gateway"
     misc_feature    10336..10383
                     /note="Frt"
     misc_feature    10384..10883
                     /note="mouse En2 intron"
     primer          complement(10452..10473)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10348..10371)
                     /note="FRT3"
     primer          14535..14553
                     /note="seq primer NF"
     misc_feature    14595..14602
                     /note="RE sites"
     misc_feature    16395..16428
                     /note="loxP"
     misc_feature    complement(14603..14634)
                     /note="Rox"
     misc_feature    complement(16355..16386)
                     /note="Rox"
     misc_feature    14635..14656
                     /note="polylinker"
     CDS             15215..15811
                     /note="puromycin N-acetyl transferase"
     misc_feature    15330..15335
                     /note="Mutated RsrII site"
     intron          10884..11258
                     /note="mouse En2 intron"
     misc_feature    11256..11258
                     /note="Splice Acceptor"
     exon            11259..11298
                     /note="mouse En2 exon"
     primer          11159..11180
                     /note="seq primer FCHK2"
     misc_feature    11299..11421
                     /note="1Dleader"
     misc_feature    11422..11473
                     /note="F2A"
     misc_feature    11569..12285
                     /note="EGFP"
     misc_feature    11479..11568
                     /note="3X NLS"
     misc_feature    14351..14589
                     /note="SV40 polyadenylation site"
     misc_feature    13384..14328
                     /note="ERT2"
     promoter        14667..15178
                     /note="PGK promoter"
     misc_feature    15922..16354
                     /note="PGK pA"
     primer          14409..14432
                     /note="LRPCR RAF5"
     misc_feature    12295..12348
                     /note="T2A"
     misc_feature    12345..12345
                     /note=" A to G to kill RsRII site"
     misc_feature    12349..13374
                     /note="Cre"
     misc_feature    11274..11299
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    11274..11289
                     /note="FCHK QC prefix region"
     misc_feature    11290..11293
                     /note="FCHK QC critical region"
     misc_feature    11294..11299
                     /note="FCHK QC suffix region"
     misc_feature    16451..16471
                     /note="B2 Gateway"
     misc_feature    10272..16501
                     /note="Synthetic Cassette"
     exon            16856..16970
                     /db_xref="ENSEMBL:ENSMUSE00000133182"
                     /type="targeted"
                     /rank="7"
                     /note="target exon 1 ENSMUSE00000133182"
     exon            17094..17266
                     /db_xref="ENSEMBL:ENSMUSE00000352771"
                     /type="targeted"
                     /rank="8"
                     /note="target exon 2 ENSMUSE00000352771"
     exon            17452..17599
                     /db_xref="ENSEMBL:ENSMUSE00000414006"
                     /type="targeted"
                     /rank="9"
                     /note="target exon 3 ENSMUSE00000414006"
     exon            17739..17875
                     /db_xref="ENSEMBL:ENSMUSE00000133186"
                     /type="targeted"
                     /rank="10"
                     /note="target exon 4 ENSMUSE00000133186"
     exon            18920..19337
                     /db_xref="ENSEMBL:ENSMUSE00000336098"
                     /type="targeted"
                     /rank="11"
                     /note="target exon 5 ENSMUSE00000336098"
     misc_feature    16502..19588
                     /note="Critical Region"
     misc_feature    19612..19645
                     /note="Downstream LoxP"
     misc_feature    19589..19668
                     /note="synthetic loxP region"
     misc_feature    19669..23115
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatctgga catcatgttt tgtctatctt tggctgttat ttgtccttgc
     4321 cttgactccc ctttcttcac cttggacttc agtatcccca aacaggagga cctgtgtgtt
     4381 ctttgaggct cctggagtgc ggggaagcac aaagacactg ggggaaatgg tagatgcagg
     4441 accaggcccc cccaagggta tccgctgcct ctacagccat tgctgctttg ggatctggaa
     4501 tcttacccat ggccgggcac aagtggagat gcaaggtgaa tgaccaagta cacggtggac
     4561 aagagctagg gcaggggaga gactagggca ggggagagta tagggcacgg gagagtctag
     4621 gataggggag aatctagggt agaggtgtgt ctaggttaag ggaggcagcc aaaagaaagg
     4681 ggagaagtgg aatgtgtaga gggaagacag gcatgtcaga tgcctttgat agagaaggga
     4741 tttgttctct gtccccaaac ttcattgaac tctctctcct tttcctcctt cccctaatcc
     4801 caccccttca ggatgccgag acagtgatga gccaggctgt gagtccctcc actgtgaccc
     4861 agtccccaga gcccacccga accccagctc cacactcttc acgtgttcct gtggcactga
     4921 cttctgcaat gccaattaca gccatctgcc tccttcggga aaccaagggg ctcctggccc
     4981 ccaggagccc caggctaccc caggtagttg ccttccccag ggtactagag cccacagtgg
     5041 gcagaagcat aggtcagagt gagaaccggg ccagttctca ccccacccca cccccgccca
     5101 aactttccct cctcttggcc ttgggaagtt ggttgccctg gtgactgggt gataaggggg
     5161 cttgtgacca gcatgggggt ggtttggaaa gcaatctctt tgaagagggg agaggttcag
     5221 tgctcaccca cacgccttct tcccttgctg tccaggtggg cccgtctgga tggcactgct
     5281 gctcctggga atgtttctcg tgttgttgct gagtagcatc atcttgggta ctaatctctc
     5341 ccatccctcc ctccctttcc ccgccccccc cccccccatg agagcaaggc acttccccaa
     5401 aggttgaagc ctcctccagg ctctgctgat cccccatttt cttttgaagc tccacatttc
     5461 cttgcaagat gccctcttcc cacatagctc ccttgtctgt ctcggtccag tgcaccctac
     5521 ctaccctaag gctcttctct acttcagccc tgctacaacg aaaggcctgc agagtgcaag
     5581 gtgggtcaga cccagagccc ggctcaggcg gagactgcag tgaggaactg ccagagctgg
     5641 ccgagctgcg tttctcccag gtgctccatc ggtgggagga gggtgggctt ctgtggcact
     5701 cctggaggtt ctgctgggga ggaagcctgg ccctggcgct agctctgagg cccacactca
     5761 acgcagcctc tctactaggt aatccaggaa ggaggtcatg cagtcgtctg ggccgggagg
     5821 cttcagggtg agatggtagc catcaaggcc ttccccccaa gggctgtggc ccagttccga
     5881 gctgagaggg ctgtgtacca gctgctaggc ctacagcatg accatatcgt tcgctttatc
     5941 actgctggcc agggaggccc tggccctctg ccctctgggc ctttgctggt actggaactg
     6001 taccctaagg tgagcaccaa gcaacatctg tcgtctgtac tactctgcac tacacctatg
     6061 gggtgctatg tggcagtgga gcctcactca ctccctcatg cctgttctcc tgcctcatac
     6121 tttccttaga atgaatatat atatatatat tctttttctt tttgaaacag tttctcagtg
     6181 tagccttggc tctcttagaa cttgttctgt agaccaggct gaccttgaac acagagagat
     6241 ctgcctgcct ctgcctccca agcactggga ttaaaggcat gttccactcc tgcctgtcta
     6301 gaagagagcc attgttaaat acatatccag gagtagctct ctaaactcat tcatgagttc
     6361 acttaaccaa tatttataaa ctatgtggca ggcacttcta gatgctgaga aaagcacaca
     6421 gggtgcctag gtttgcaggt gtcatagact aacgggtcat agaaagggat caacacaaaa
     6481 ggaagttgca aatgtcaagt gtgctgaagg acaggacact gggaacagat gatagattca
     6541 acctgagggg ctgtcgagat gcctacttat tgtgcaagcc ttggggaccc gagttcagtc
     6601 cccagaaccc cagtcaaggt agaaggagag aactctacaa agttgttgtc ttagacaggg
     6661 tttctttttc ttttcttttc ttttcttttc ttttcttttc ttttcttttc tttttctttt
     6721 tcttttcttt tttctttttt tttttttttt tttttttggg cttctctgtg tagctctggc
     6781 tgtcctggaa ctcactttgt agaccagact ggcctcgaac tcagaaattc tcctgcctct
     6841 gcctcctgag tgctgggatt agaggcgtgc gccaccatgc ctggctttag tcagggtttc
     6901 tattcctaca caaacatcat gaccaaatag caagttgggg gttgcggggg atgggtttat
     6961 tcagcttaca aaattccaca ctgccattca tcaccaaagg aagtctgtag ctcaaacagg
     7021 tcgggaagca ggagctgatg ctgaggccat ggagggatgt tacttactgg cttgcttccc
     7081 ctggctcact cagcttgctt tcttatagaa cccaagacta ccacccacaa tgggccctcc
     7141 cacccttgat cactaattga gaaaatgcct tatagttgga tctcatggag gcatttcctc
     7201 aagggagtct cctttctttg tgataactcc agcttgtgcc aagttgacat acaaaaccag
     7261 ccattacagt tgttttctga ccaccacaat ccattctggt atggcctccc aaagacttcc
     7321 cctccagtaa taattttaaa aatgtgtata ggtgtttcac ctgtacgtat gtctgtgcac
     7381 tgtggcatgc taggtgccca tggaagtcag gagaggatat taacactcct ggggctctca
     7441 acgtagctgt gatgtgagta ctgggaatta aactttagtc ccctagaaga gcagacagta
     7501 taactcctga gccatttctc ctagttttaa taaaaagaaa agatgtatgt aacctgaggt
     7561 gagagattaa caatgtgaaa aggtaacaac gtgaaaagga ggaaagctgg gtagtaggac
     7621 ttgactataa tcctcgtact tgggaggctg aagtgagagg actgaggtga attccaggct
     7681 agcctgggct acatatgatc ctgactcgaa gaaataaaac aggcctgagt gcagcggatg
     7741 ctccctagac cccagcatta gggaggcaga ggcaggcata tctatgtgag ttcaaaacca
     7801 gccaaggtta caaagtaaga ccctatccaa cacaaaccaa aacccccaaa acaaaagaaa
     7861 ataagagtga agaagaaaac ataggcagaa gtggggccat atggagcaga gctgctgaag
     7921 actggggtgg gactctaggc actttgaaca cattcccata cttaatcttt agaaggatac
     7981 tacgaggtag acattgtatc catagagaag aaagggagtc tcgtttgctc aggctctgtg
     8041 actggagagt ctggctcttc gcagatctgc ggctatctcc tgtttatcca gtgtcagcac
     8101 catctgttcc tttgaaggaa ctctgcctgg cgcctttgcc ctgattgctg cagctcctaa
     8161 cttgcctaaa tgcggtggct ctttcccagg ctttaccaac ctttacaact ccttcattgc
     8221 tgagatcccg agttctggaa acttcctttt ttgaccacat tcttcttggg tcaattgaaa
     8281 aacaactttc cggcctgggg atatatctta gccaataaag tgccttttgt gcatgagggc
     8341 ccgagttcag ttctcagaaa taagttttaa gaagccagat ttgggacagg agtcatggtt
     8401 caggagttaa gagcactggc taccctttca gaggacccaa gctcccagca cctatgccag
     8461 ctgtcacaat tgcctgtaac tccatctcca ggggatgcaa tggcctcttc tggtctccat
     8521 tggcacctgc aacacacatt cacacacaaa gaaacaatac ccatatttag gttttttgtt
     8581 ttgttttgtt ctttgagaca gggtttctct gtatagccct ggccgtcctg gaactcactt
     8641 tgtagaccag gctggcctcg aactcagaaa tccgcctgcc tttgcctccg gagtgctggg
     8701 attaaaggca tgtgccacca cgaccggctt aatttttttt ttaagtttat ttttatttat
     8761 ggattgtgct cctttctctt tgtgggcatg tgcacataga tgtgggtgct ctcagattaa
     8821 aggcatcaga tcccctgcag ctggagttag aggcagtgta ttgcttatgt gggtcctgag
     8881 agttcaactc tgacccacta aaagagcagt gtgccactga gcactgactc acttctctag
     8941 cccaaagtac attaattgtg tttgtttgtt ggagacaggg cctctctaag tagccctggc
     9001 tattctgaaa ctcactctgt agaccaggta cgccttgcaa agatccacct gcctctgcct
     9061 ctgcctctgc tatacagaga aaccctgtct cggaaaaaac aacaaaaaaa aagaaggcca
     9121 ggttctttct ttatctcttt cttctttctt tctttctctc ttctttcttc cttcctttct
     9181 tttttccttc cttccttctt tccttccttc ctatactgca atttatttat ctatttttat
     9241 ttatatgtgt acactgtagc tgtcttcaga cacaccagaa gagggcgtca gatgccatta
     9301 cagatggttg tgagccacca tgtggttgct gggaattgaa ctcaggacct ctggaagaac
     9361 agtctgtgct cttaaccact gagccatctc tccagcccag ttcattttca tattgtggca
     9421 ttcattcagt tctctacttc tatcatgctc tcctcctcct gagacacagt gctgcctggg
     9481 tacaactgtg gcaaactaag ggtgttcagt atgttcatga atagcctatt cccagttgcc
     9541 cctcctcctt tggctccacc catctgtatc tacgcaggcc tgttagccca ggtcaggtgg
     9601 ttggagggac agaggtcctt gaccactttt gtcttgggtc ctctgagtca gcgtgtgaca
     9661 gcgactctta gtcagattgc ttggatttac ttggattaca actccattgt ccgacctcaa
     9721 gaaagcttac tcagcatttg ctaaagctcc acctccagcc cccgaggtag cttgtgcctg
     9781 tgctgctggc actgggaggt agaggcagga gaatcactgc aaccatgaaa gcagtcttcc
     9841 acgtcagcta cggtggcata caaaccagtg agatcctgcc taaaagaagg tggaagggaa
     9901 ggagaaaaag ggccagcaat gtcatgggca aaggtccttc tgtgaagctt ccacatgacg
     9961 gcctgaggca gtccctggag cccagaaggt gaaaggagaa aaccaactct agcaagttgt
    10021 gttttgacct ccacaggtgc catgtagtgt gtggggaccc acataccccc acacaaataa
    10081 ataagtgtac tttttttttt tttaaaaata aaggagaggg gaaaaaaaaa agaaagaata
    10141 aaagacctac aaaaatcagg gtaatcagaa tgccgtcacc atggggtcct catgaggtct
    10201 atcggagacc ctgttctaag gacctgggtc acagcaaagt accccgtacc actgaaataa
    10261 actactaatc caaggcgcat aacgatacca cgatatcaac aagtttgtac aaaaaagcag
    10321 gctggcgccg gaaccgaagt tcctattccg aagttcctat tctctagaaa gtataggaac
    10381 ttcgaaccct ttcccacacc accctccaca cttgccccaa acactgccaa ctatgtagga
    10441 ggaaggggtt gggactaaca gaagaacccg ttgtggggaa gctgttggga gggtcacttt
    10501 atgttcttgc ccaaggtcag ttgggtggcc tgcttctgat gaggtggtcc caaggtctgg
    10561 ggtagaaggt gagagggaca ggccaccaag gtcagccccc cccccctatc ccataggagc
    10621 caggtccctc tcctggacag gaagactgaa ggggagatgc cagagactca gtgaagcctg
    10681 gggtacccta ttggagtcct tcaaggaaac aaacttggcc tcaccaggcc tcagccttgg
    10741 ctcctcctgg gaactctact gcccttggga tccccttgta gttgtgggtt acataggaag
    10801 ggggacggga ttccccttga ctggctagcc tactcttttc ttcagtcttc tccatctcct
    10861 ctcacctgtc tctcgaccct ttccctagga tagacttgga aaaagataag gggagaaaac
    10921 aaatgcaaac gaggccagaa agattttggc tgggcattcc ttccgctagc ttttattggg
    10981 atcccctagt ttgtgatagg ccttttagct acatctgcca atccatctca ttttcacaca
    11041 cacacacacc actttccttc tggtcagtgg gcacatgtcc agcctcaagt ttatatcacc
    11101 acccccaatg cccaacactt gtatggcctt gggcgggtca tccccccccc cacccccagt
    11161 atctgcaacc tcaagctagc ttgggtgcgt tggttgtgga taagtagcta gactccagca
    11221 accagtaacc tctgcccttt ctcctccatg acaaccaggc tcccaggtcc cgaaaaccaa
    11281 agaagaagaa cgcagatctc tagagtcacc gagttgcttt accggatgaa gagggccgaa
    11341 acatactgtc caaggccctt gctggcaatc cacccaactg aagccagaca caaacagaaa
    11401 attgtggcac cggtgaaaca gactttgaat tttgaccttc tcaagttggc gggagacgtc
    11461 gagtccaacc ccggaccggt gcatgtggac cccaagaaaa agcggaaggt ggaccctaaa
    11521 aagaaacgca aagtggatcc taagaaaaaa agaaaggtgc acgtgtccat ggtgtccaag
    11581 ggcgaggaac tgttcaccgg cgtggtgccc atcctggtgg aactggatgg cgacgtgaac
    11641 ggccacaagt tcagcgtgtc cggcgagggc gaaggcgacg ccacatacgg aaagctgacc
    11701 ctgaagttca tctgcaccac cggcaagctg cccgtgcctt ggcctaccct cgtgaccaca
    11761 ctgacctacg gcgtgcagtg cttcagcaga taccccgacc atatgaagca gcacgacttc
    11821 ttcaagagcg ccatgcccga gggctacgtg caggaaagaa ccatcttctt taaggacgac
    11881 ggcaactaca agaccagggc cgaagtgaag ttcgagggcg acaccctcgt gaacagaatc
    11941 gagctgaagg gcatcgactt caaagaggac ggcaacatcc tgggccacaa gctggagtac
    12001 aactacaaca gccacaacgt gtacatcatg gccgacaagc agaaaaacgg catcaaagtg
    12061 aacttcaaga tccggcacaa catcgaggac ggctccgtgc agctggccga ccactaccag
    12121 cagaacaccc ctatcggcga cggccctgtg ctgctgcctg acaaccacta cctgagcacc
    12181 cagagcgccc tgagcaagga ccccaacgag aagagggacc acatggtgct gctggaattc
    12241 gtgaccgccg ctggcatcac cctgggcatg gacgagctgt acaagcctgc aggcgagggc
    12301 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccgggccgtc caatttactg
    12361 accgtacacc aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag
    12421 aacctgatgg acatgttcag ggatcgccag gcgttttctg agcatacctg gaaaatgctt
    12481 ctgtccgttt gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa atggtttccc
    12541 gcagaacctg aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta
    12601 aaaactatcc agcaacattt gggccagcta aacatgcttc atcgtcggtc tgggctgcca
    12661 cgaccaagtg acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt
    12721 gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggtt
    12781 cgttcactca tggaaaatag cgaccgctgc caggatataa ggaatctggc atttctgggg
    12841 attgcttata acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc
    12901 tcacgtactg acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc
    12961 accgcaggtg tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt
    13021 tccgtctctg gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat
    13081 ggtgttgccg cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt
    13141 gaagcaactc atcgattgat ttacggcgct aaggatgact ctggtcagag atacctggcc
    13201 tggtctggac acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca
    13261 ataccggaga tcatgcaagc tggtggctgg accaatgtaa atattgtcat gaactatatc
    13321 cgtaacctgg atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg cgatctcgag
    13381 ccatctgctg gagacatgag agctgccaac ctttggccaa gcccgctcat gatcaaacgc
    13441 tctaagaaga acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg
    13501 gatgctgagc cccccatact ctattccgag tatgatccta ccagaccctt cagtgaagct
    13561 tcgatgatgg gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg
    13621 gcgaagaggg tgccaggctt tgtggatttg accctccatg atcaggtcca ccttctagaa
    13681 tgtgcctggc tagagatcct gatgattggt ctcgtctggc gctccatgga gcacccagtg
    13741 aagctactgt ttgctcctaa cttgctcttg gacaggaacc agggaaaatg tgtagagggc
    13801 atggtggaga tcttcgacat gctgctggct acatcatctc ggttccgcat gatgaatctg
    13861 cagggagagg agtttgtgtg cctcaaatct attattttgc ttaattctgg agtgtacaca
    13921 tttctgtcca gcaccctgaa gtctctggaa gagaaggacc atatccaccg agtcctggac
    13981 aagatcacag acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag
    14041 caccagcggc tggcccagct cctcctcatc ctctcccaca tcaggcacat gagtaacaaa
    14101 ggcatggagc atctgtacag catgaagtgc aagaacgtgg tgcccctcta tgacctgctg
    14161 ctggaggcgg cggacgccca ccgcctacat gcgcccacta gccgtggagg ggcatccgtg
    14221 gaggagacgg accaaagcca cttggccact gcgggctcta cttcatcgca ttccttgcaa
    14281 aagtattaca tcacggggga ggcagagggt ttccctgcca cagcttgatg aagatctgag
    14341 ctcttaatta atgatcataa tcagccatat cacatctgta gaggttttac ttgctttaaa
    14401 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
    14461 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    14521 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    14581 tcatgtctgg atccgtttaa actaacttta aataattggc attatttaaa gttacatatg
    14641 gcggccgccc taaggctcga cctcgaaatt ctaccgggta ggggaggcgc ttttcccaag
    14701 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
    14761 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
    14821 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
    14881 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
    14941 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
    15001 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
    15061 cgggctcagg ggcggggcgg gcgcccgaag gtcttccgga ggcccggcat tctgcacgct
    15121 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
    15181 catccatcta gatctcgagc agctgaagct taccatgacc gagtacaagc ccacggtgcg
    15241 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
    15301 ctaccccgcc acgcgccaca ccgtcgatcc agatcgccac atcgagcggg tcaccgagct
    15361 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
    15421 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
    15481 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
    15541 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
    15601 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
    15661 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
    15721 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
    15781 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
    15841 gaccgaaagg agcgcacgac cccatgcatc gatgatatca gatccccggg atgcagaaat
    15901 tgatgatcta ttaaacaata aagatgtcca ctaaaatgga agtttttcct gtcatacttt
    15961 gttaagaagg gtgagaacag agtacctaca ttttgaatgg aaggattgga gctacggggg
    16021 tgggggtggg gtgggattag ataaatgcnt gctctttact gaaggctctt tactattgct
    16081 ttatgataat gtttcatagt tggatatcat aatttaaaca agcaaaacca aattaagggc
    16141 cagctcattc ctcccactca tgatctatag atctatagat ctctcgtggg atcattgttt
    16201 ttctcttgat tcccactttg tggttctaag tactgtggtt tccaaatgtg tcagtttcat
    16261 agcctgaaga acgagatcag cagcctctgt tccacataca cttcattctc agtattgttt
    16321 tgccaagttc taattccatc agaagctggt cgagtaactt taaataattg gcattattta
    16381 aagttaggcg cgccataact tcgtatagca tacattatac gaagttatgt cgagatattt
    16441 aaatattaga cccagctttc ttgtacaaag tggttgatat ctctatagtc gcagtaggcg
    16501 gtgaggttac atccatgtgc tactatgtct ggctaaaatt ataacgtgtg tgtatgtgcg
    16561 cgcgcacgtg cagactggca tgttcttgat ccccattcct tccatttctc aagtgccggg
    16621 gtggcagaca cgctgtacca cgcccaacca gagttactat tttttgctgt cactttgctg
    16681 catattctca tgcttccacc gtctgtagtg tcttgacctg gttctgtcag ggtgtctctg
    16741 tctgctgggg aggtgcgggc tggggtgtgt gtgggggggt ctgtcagtag ctctctgctt
    16801 cctggaatga acctcctgag ctctgtgcac cctgctcctc tctgcttttc tcaagggctc
    16861 cttatgccac tacttgactc agtacacgag cgactgggga agttccttga ggatggctct
    16921 gtccctggca gagggcttgg cttttctcca cgaggagcgt tggcaggatg gtgagtatcc
    16981 ggcaacaggg agggaggagt cagtagccgc acccgatgcg tgcaggccca gttctctctc
    17041 cgcttatggg agaaggactg caccaaggag aaccacatga cctgtgtccg caggccagta
    17101 caaaccaggt atcgcccacc gagacctgag cagccagaat gtgctcattc gggaagatag
    17161 gtcatgcgcc atcggggacc tgggccttgc cttggtactc cctggtcttg ctcagccccc
    17221 tgccttggcc cctactcaac cccgaggccc cgctgccatt ttggaggtga gtgtttttga
    17281 taagtgtgag acctgtggtg acggtggctc tgatgacagt acaacttttg ctgtggcaac
    17341 ggtgcggctt tgctagtaga cagagcattg ctgtaattgg ggaatttgtg gaccagagct
    17401 gcaagacagg ttgccataga ggtgactcct ggcactccac atcttctcca ggctggcacc
    17461 cagcggtaca tggctccaga gctcttggac aagactctag acttgcagga ctggggtaca
    17521 gctctccaga gagcggatgt ttactctctg gcgctactac tgtgggagat cctgagccgc
    17581 tgttccgatt tgaggcctgg taaagtctac ccatagtgtc ccacctaaca aaggctcacg
    17641 tgccttctat tccagaaggc tatgatctcc cctagtttta tcacctggga gccccctcgc
    17701 cagggtaggt taacagtcta tgtctatacc tttcccagac cacagaccac cacctttcca
    17761 attggcttat gaggcagaac tgggcagcaa tcccagtgcc tgtgagctct gggccctggc
    17821 agtggaggag aggaagcgcc ccaacatccc atccacttgg agctgctctg ccacagtaag
    17881 aagccatagg cttgccagct ggggaccggg agtcagggct gagtttggat ttcaaggact
    17941 tctctgccac aaccagacct tctcctccta tcctgagcct cagtttacct tttcttctac
    18001 aagtgtcaca cagttggttc cacccacata ccaccctgtg gagccttttt attcctatcc
    18061 ccttactcca cagttaactc tgttgttcag tcgtctcctc caggaagtcc tctctgactt
    18121 ttctcttaca tggacctcag cccctttgac agtatctctc tctctcttta tttatttatt
    18181 tatttattta tttatttagg tttttggaga cagggtttct ctgagtagcc ctggctgttc
    18241 tggaactcac tctgtagacc aagctggcct caaactcaga aatccacctg cctttacctc
    18301 ccaagtgctg ggattaaagg tgtgcttcac cactgcccgg cgacagtatc tctttaggaa
    18361 tagtgtaccc caaagttcaa atcagtgccc catcattttg tactatgcag ctctgtttct
    18421 actgtggacc aattaaagcc agctctaggt ataacaaggt cagaagggtc acctgatagt
    18481 gaacgcagag ctcttcacaa atggtttcct gagatgtccc ttctcccact ctcctacctg
    18541 aaatgtctac catactagtg tctattggat tggtctgtgg aagccaggct ttgtctgcag
    18601 gacacactgc ttgactaaga gaaggaagag taactttcca ttagcacaaa agttgtcagc
    18661 ctgaattatc cttagaggaa aaggtagacc cgttggaagg tatcccagga gtctgtctca
    18721 gggaacaaac tcaagggtct agactttagg gaatggggct tggaggcctt ccacaggtgg
    18781 ggtgcggagt caggagtggt gagacatgct cttagcaagg actccgtgca gaatggaacg
    18841 gagtgcgtgg gagaagtgaa gactggagat gtctgctctg gggttggcca ggcctgccat
    18901 cttctctctc tccctccagg accctcgggg gctgagggag ctcctggaag attgctggga
    18961 cgcagaccca gaagcacggc tgacagctga gtgtgtgcag cagcgcctgg cagccctggc
    19021 ttaccctcac ggggcgtcct ctttcccaga gagtccacag ggctgcccag agaactgcct
    19081 ttcagctccc gcctctgccg ttttcccctg tcggcctcag cagagttctt gtctcctcag
    19141 tgttcagcaa ggccctggct ctaggagtcc cgatcctgta ggcgacactg ttcaggtgta
    19201 tgtaaatgag tagcttgttt gaactctctg tacaggccga catgcgtgtg gccgacatgc
    19261 ctactgctgt cattctctgt gggtttctaa cccatggggt agatatgctc aggaaagaga
    19321 ataaataagg ttcccgcaca ctcttcatca atgtgttttt acttctttaa gatccgttta
    19381 ttttattcat agtacgtgtg tttgtgagtt tgtgtgtgcc acatacgtgc aggaggttgt
    19441 ggaggtcaga agaagatgtc aaagctcctg gaactggagt cacaagcagt tgttaatcac
    19501 catgtgggtg ggtgctggga ataggtcctt tgcaagagca gtaattgctc tgagctccta
    19561 agccatctct ttagcccttt attttattga gatggcgcaa cgcaattaat gataacttcg
    19621 tatagcatac attatacgaa gttatggtct gagctcgcca tcagttcaac tcactttgta
    19681 gaccaggctg gcctcgaact cggaactcaa gagatccacc tgcctctgcc tcccgagtgc
    19741 tgggattaaa ggcatgcgcc accacgcccg gctagccctt tattatttat ttatttattt
    19801 atttatttat ttatttttga ggcagggtct tactgtgtgg ccttggctta cttacacctg
    19861 ctatgcagta tgtagcccag gctagtctct gactctcgaa acaatcctct tgcctcagca
    19921 tctggagcac atgcttgaga cactagcatt tacttactta ttagacaagg tctcactatg
    19981 tagctctggc tggccaggaa ctcactatgt agaccaggtt ggccttgaac tcacagaaat
    20041 ctacctgcct ctctctttct tttttttttt tttttttttt ttgggggggg gtttcttttt
    20101 ggtttttcga gacagggttt ctctgtatag ccctcgctgt cctggaactc actttgtaga
    20161 ccagggtggc ctcgaactca aaaatccacc tgcctctgcc tcccaagtgc tgggattaaa
    20221 ggcgtgagcc accacgcctg gctctctctc tctctttcaa gtgatagaat taaaggtgtg
    20281 tgcccctgtg gcaggtatca acattttttt tttttttttt gagacatggt ttctctgtat
    20341 agccctggct gttctggaac tcactttgta gaccaggcta gcctcgaact cagaaatccg
    20401 cctgcctttg cctcccaaat gctgggatta aaggcgtgcg ccaccacgcc cggctccaac
    20461 atttattttt gaagacttta tttatatcat tttaaaactc tctgtctctg tctctctctg
    20521 tgtctgtctg tctgtctgtc tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtggtgt
    20581 acagaggggc actggtaagg tactggatac ccctggagtc gggggagtta ggagccactc
    20641 aatatgaatg ctgagtactg aactccagtg ctctggaaca gctgtgtatg ctttttaatc
    20701 tctaagtcat ctctctagga ccaccaggac taattttcac atggtggcac cacctgttgg
    20761 gcattggagg agtggccaca gtgaagccat tggcctgagt gcactcagat cgctgaagct
    20821 cacactgtgg ggatctgtcc agactcaggg aggtgagcga ggttacctag gcagcagttg
    20881 cccaggagca ccagggggtg gcgctatacc tcgcttggca tagtacttac agaacattcg
    20941 ggaagccctg ggttccatcc ctagcactgc ataagcagtg tggcaggtcc ttgtaatcct
    21001 agcacaggga aggtggaggc cggagctgat ctggagctgt aggcaggcag ggaggatcaa
    21061 catttcaaag tcagaggctg gagagagagg gctaagttag gagcacttgc tgctctttca
    21121 gggaaccagg gtttagtttc cagtgctcct attagcaagt tcacagatgc ccataattct
    21181 cgctctaggg tacccgaggc cctcttcggg cctctgtggg cacctccatg cacaggtgca
    21241 catacagacg aacagacaga caaacagcac atatactaca ttgcttctac agcacaaaca
    21301 ctaaattaaa acaatacaga gattattgtt gtccctgtac aagggtaacc cacaaagttg
    21361 ggaagcattt catatatttt aaaaagcttt ctttaaaaaa ataaatatat atgttttcct
    21421 tcatattcta tacattaaga atacaatttt gggatggaga gatggctcaa cagttaagag
    21481 caatgaccac tcttccagag gtcctgagtt caacccccaa caaccacacg gtggctcaca
    21541 accatctgta atgggatctg attaccctct cctggtgttt ctgaaaatgg cttcagtgta
    21601 ctcttataca tccccgaatg tatacattaa gaataaattt tcaagccagg cgtggtggca
    21661 cacgccttta atcccagcac tttggaggca gaggcaggca gatttctgag ttcgaggcca
    21721 gcctggtcta caaagtgagt tccaggacag ccagggctac gcagagaaac cctgtctcgg
    21781 aaaaagcaaa aaaaaaaaaa aaaaaagaat aaattttcat cttaccctaa tgtattaatt
    21841 tctatctata aatatatagg cagagatata gatgcaggta agcaggattt attagaatga
    21901 tttacaggct gcaacccagc aaatccaaca atgactggct gtaagcagga agtccaacag
    21961 tccagtacag tccacgtgct tggatgtctt cagtacatgg tggactccca aagaactagg
    22021 ctttaatgcc ggtaaagaaa tggactttcc tttccaggag agaacaaact ggccaagagc
    22081 aaagtgtcct tcttcagtgt ccttatctag gtttccagca gatggtatgg cccaggttag
    22141 aggtgtgtct tcccacttca aaatctagat taagtaatct attcacctac ttcaggttaa
    22201 gaagaaaaaa aaaaaggtgt gtcccgacaa ccaagaatag ttatcacacc taataaaaat
    22261 tgttgtaaat atttgtaaaa gtttgagatc atcttggcaa catagcaggc tgaggccatc
    22321 ctaaactgag tgtgagatcc tgtctcaaaa ataaataaaa gtgccgggca tggtggcgca
    22381 tgcctttaat cccagccctc gggagggaga ggcaggagga tttctgagtt cgaggccagc
    22441 ctggtctaca gagtgagttc caggacagcc agggctatac agagaatacc ctgtcttgga
    22501 aaaaaaataa aaaaaaataa aataaaataa aataaaataa aataaaataa aagtgtagag
    22561 ggtggggaga ggaatgtaga cagctaaggt ccaggccttg tgtggcttgt gggaaagtcc
    22621 ttgatttatt catcatttta tctatgaaca gggtctcatg tagcgcagga tgacctaaaa
    22681 ctctctgtgt tgccaagggt gaccttgaac tcaggatcct gtaagcccta tctgcaaagt
    22741 gctggggtta gctgggcttg gtggcccaca cctttaaccc tagcactcgg gaggcagagg
    22801 caggtggatt tctgagttcg aggccagcct ggtctacaaa gtgagttcca ggacagccag
    22861 ggctatacag agaaactctg tctcgaaaaa ccaaaaaaaa aaaattctga gagttgtggc
    22921 ttgcttttgt ttttctagca cccgagagag agagagagag agagagagag agagagagag
    22981 agagatatgg gatgactgct tttcatccaa agtcaaccca agtcatactg gtagattgtc
    23041 tcaaaaacat caaaaccaaa aatgaattca cttagttacc cagaacaatg acatgaaact
    23101 ataactgcac catct
//