LOCUS       209933#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20282 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 209933
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000164044
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8535..8669
                     /db_xref="ENSEMBL:ENSMUSE00000916659"
                     /rank="1"
                     /note="ENSMUSE00000916659"
     misc_feature    4275..8904
                     /note="5 arm"
     misc_feature    8932..8956
                     /note="B1 Gateway"
     misc_feature    8969..9016
                     /note="Frt"
     misc_feature    9017..9516
                     /note="mouse En2 intron"
     primer          complement(9085..9106)
                     /note="LRPCR LAR3_EN2"
     primer          complement(8981..9004)
                     /note="FRT3"
     primer          13182..13200
                     /note="seq primer NF"
     misc_feature    13242..13249
                     /note="RE sites"
     misc_feature    15042..15075
                     /note="loxP"
     misc_feature    complement(13250..13281)
                     /note="Rox"
     misc_feature    complement(15002..15033)
                     /note="Rox"
     misc_feature    13282..13303
                     /note="polylinker"
     CDS             13862..14458
                     /note="puromycin N-acetyl transferase"
     misc_feature    13977..13982
                     /note="Mutated RsrII site"
     intron          9517..9891
                     /note="mouse En2 intron"
     misc_feature    9889..9891
                     /note="Splice Acceptor"
     exon            9892..9945
                     /note="mouse En2 exon"
     primer          9792..9813
                     /note="seq primer FCHK2"
     misc_feature    9946..10068
                     /note="1Dleader"
     misc_feature    10069..10120
                     /note="F2A"
     misc_feature    10216..10932
                     /note="EGFP"
     misc_feature    10126..10215
                     /note="3X NLS"
     misc_feature    12998..13236
                     /note="SV40 polyadenylation site"
     misc_feature    12031..12975
                     /note="ERT2"
     promoter        13314..13825
                     /note="PGK promoter"
     misc_feature    14569..15001
                     /note="PGK pA"
     primer          13056..13079
                     /note="LRPCR RAF5"
     misc_feature    10942..10995
                     /note="T2A"
     misc_feature    10992..10992
                     /note="A to G to kill RsRII site"
     misc_feature    10996..12021
                     /note="Cre"
     misc_feature    9907..9946
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    9907..9922
                     /note="FCHK QC prefix region"
     misc_feature    9923..9940
                     /note="FCHK QC critical region"
     misc_feature    9941..9946
                     /note="FCHK QC suffix region"
     misc_feature    15098..15118
                     /note="B2 Gateway"
     misc_feature    8905..15148
                     /note="Synthetic Cassette"
     exon            16175..16432
                     /db_xref="ENSEMBL:ENSMUSE00000161653"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000161653"
     exon            15517..15537
                     /db_xref="ENSEMBL:ENSMUSE00000161652"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 2 ENSMUSE00000161652"
     misc_feature    15149..16598
                     /note="Critical Region"
     misc_feature    16622..16655
                     /note="Downstream LoxP"
     misc_feature    16599..16678
                     /note="synthetic loxP region"
     exon            19950..20204
                     /db_xref="ENSEMBL:ENSMUSE00001226444"
                     /rank="4"
                     /note="ENSMUSE00001226444"
     misc_feature    16679..20282
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggattagac cctcaaataa ctggacctgc taagtgacca cagcattgct
     4321 gtccccctac cccttccagg atggagaata ggctctgggt gttcacactg gctgctggcc
     4381 tttttgaagg catcacagca ggattgtggg acccaccttg tggtcctggg cctgctgagg
     4441 tttattgatg gggaaagttc tttgttactt ggaaggcatt gtatggtaga gtgaccttga
     4501 ctcccaggag tggttcgact ttgattccgt tgacagtcag ttatgggctt tgagtattgc
     4561 aaagtaagta ttgggcaaga atgcaaaaac cctctgtgcc tgggttcctt gagcgttttt
     4621 cctggctggt gccgagagaa gagaggcaca gtaagggaag tggtcagtag aaggttgagg
     4681 gcagatcaga cacattgtta gcttttactc tgcagagaag gagaaaactg caagggcttt
     4741 tccagtgtag gaatgatgtg gcatggcttg ggtttaagaa ggtgattctg cacgttgtgt
     4801 tgagaacaga ctggagtata gcaaggatag aagagcgaag aagagggcta ttgcagtaac
     4861 ccagcttgcc gaagatattt gtctctgcaa gaatggcgtg tagagactaa aagtgttaga
     4921 ttccaggtta aatggcgtgt agaggagatt aaaagtgtta gattccaggt taaatagaat
     4981 caccaggtat gacaacgttc ctgactaact tgatatggta acaaaaatga aatgggagac
     5041 tggttgattc tacagttttt gttttaatta ccaatattcc aggttggttt tgttgttgat
     5101 ggtggtggtt tttgtttggt tggtttggtt tttttttttt ttttttttgg taccatttaa
     5161 aaataaaaca cttaatgttt taaaactgat caatgctttg gggatagaga gacaacatgg
     5221 gggttaagag cacttattgc tctttcagag ttcaattccc agcatctagt gaagaggttc
     5281 acaactgtct gtaactcctg ttatgatctg aacccccacc cacccaccac ccaccccacc
     5341 agaatagctg tagccatgtt tattccatgt ctaccagtgc aatagctgta atgaaagacc
     5401 cacaacatga ggactccccc acgttctgac tcaggagagg caacacccca aaatcaccaa
     5461 cgagaaacag tcttgctgca aactgcaaga ggatttttat tcaagagcgc tctcgggccc
     5521 acggtcatac accaggggta gaggactgtg gcaccccgag tagctggata agggggtatt
     5581 taaaggaaga aaccacaact caaggaagtg gggagggcgt tgttggaaaa taccaaaaat
     5641 accagttaag agtcacaagg aagtacaaag tcacaggtgt cacaaggaat acctggtaat
     5701 tgtgagggtt atttctcaag acagtttcta agagccccta acaatagcac ctttgcatag
     5761 cgggttccag caatggtcag ggtgggtcag ggtgactttc tttgaatgaa cactccttga
     5821 acccaggaag cgggtgggtg gaggaatgtc gatatctgtt ttatgattag cataccttgg
     5881 agcactgagt ttacctactc tttcagtaac atgcctgcca cgttgctgta acatgcctac
     5941 tggtcactga cattgataag tacttggctc aaggccatgc tgaccacata ccctgttttt
     6001 gttcttaatg ttctgtatga ggtttgctaa ttttaagaaa ttacacaaag ctttatgtag
     6061 gacccctcaa atcaaaggtc aatatgaact gttatgtcta aaaccgcttg agtcagagct
     6121 gaccgtctgg tcctttttgc acctacatat gaactcactg tggtttttgt ggctgatgct
     6181 gaagaatgcc tactaataag ctaaaaagtt acaatactct gttatctcaa tgttttgaaa
     6241 ttcccctgtt cactacccat ccatcttact caatactttg tctagtaagc caactgctct
     6301 cttccttgca gtttaaactt ttgaacccgt tttcctccta taaaaagtct actctgagag
     6361 cagactagtg ccacaattac gtttttcatt cttgtggacc tggaggtcca gtatcatgat
     6421 atgcgttcaa taaactcttc ttgcttaact aagatggtgc atttatggtt tgagtgaatt
     6481 ccttaacccc aacactccag ttccaaggga tccaacactc ttctggacct tcaggtacct
     6541 gtagtcacat actcacacac agacacccat ataaagataa ttttaaaata agtattatga
     6601 aaacaaccag tttcttattt tctctttgga gttaattgag ggtaaattga acattctagc
     6661 ttgggctttc ctgcttattc agtccgagtg gaatgtggag cctgtggaga ttttaatgtt
     6721 ggaagaataa acgtgagatt taagaggctg atgcttcttg tgttgactgg cataaaatga
     6781 agaaagataa ggtactatac agagataaga aaatgccaga aaagtaataa gactgttata
     6841 aagaacactg tagtcatcaa gggttccctc ttggtgtctg taagagagat taggcataac
     6901 ctattagtcc tgagtttgtt aaccccaact gacaaagcta aggtggtcca gaaatcaccc
     6961 cttaccccac acaagtcgtc tattatatac tttacgtcag aaaagactgt agaagtgctg
     7021 gggacttgga ggttttagcc cagtgcacag gaatgagctt gcttatttct tttcgaaact
     7081 agaagttttc taaataatta ttttcctaca ctgtagcctc tcccagcctt gagcaggctg
     7141 gctcagacct gttctgccac tgtgaaccag accacctagg gtggagtctt cggacctaga
     7201 taggtctatt tgttctgcca ctgcactctt ctccaagaca aggctacgcg cagagaggct
     7261 gaacctgttt gcctgagaca ttcctgagat aagctgacaa cctggcgacc gaccgagtgc
     7321 taacgactct ggcagaccaa gtgctgacag gctggcgaca aaccttcaaa gcctgatgtg
     7381 gcaggagatg gcaggaagaa gatctgttta tctcaccttc cccctttgta agctcagact
     7441 cttaagtaaa cattgggcct tgataggtat ctgtcttggt ctcggtattt ccgtttcctc
     7501 ccatacagct ccagcctccc cccaccccca ccccccccag aaacctagtt cctgtggctg
     7561 agggcggcta cactacacat ttgatatatt gtatatgtag tagccactag ccttgaagta
     7621 cttaaaattg agtctgaatt gaaatatgtt caaggtaaaa caatatgtga gtgcttggaa
     7681 attaaaaact gacagccggg catggtggtg caggccttta atcccagcat ttgggaggca
     7741 gaggcagagg caggcggatt tctgagttcg aggccagcct gatctacaga gtgagttcca
     7801 ggacagccag ggctacacag agaaaccctg tctcaaaaac aaacaaaaac aacaaaacaa
     7861 aacaaacaaa caaaaaaccc aaaacctttg cttttgctct gggcctggtg gcgtgtgcct
     7921 ttaaccccag tactcaggtg cagaggcaag ctgatctctt tgagctccag ggcagcctgg
     7981 ctgatatatg gttttcagga cagtctgggc tacagagaga gacaatgcct caaaaatcaa
     8041 aataaaccaa agtcatcccc caacaagttt gttatgcgtg tggaggccaa ggtcagacac
     8101 ttgcatatag gatagagacc ttcttggact tatttcttca gatgataata ataatactaa
     8161 tccagtgttt ctttcaatta cctgtttaga agatctgttt atctcaccta ggtcaaagtt
     8221 tctttgctga agctgccttg agcctttctg cattggcctg tgaggcagtc caggagttta
     8281 tgaaaacagc atcgaatcaa gactcggggc agtaagtaaa tagaggctct ctcctctgct
     8341 ttctagtgtt tctcccagtt tcttcacagc aaacagtagc ttggggtttt ccccaagggt
     8401 acaagaaggt gctgcgtgta gaagggatat ctgtcaggct gagtccacat cttgtttaac
     8461 ttgttattgt taagttaaga gaagcagagt ctgctgacca gtgcggttaa gcaagagagt
     8521 ccatttcctt cctctttttg tagttcatct cctgaacctc atcagactct gatccagttc
     8581 ttgaatgact ttggacaccc agatgtttca gaatgcacac tctggaagcc aatggctact
     8641 tccaccactg acaattctgc tgctgtttgg tgagtaagtc tcattctgaa atccaataag
     8701 ttcagcctct cttactgtct agactccagg gaccagggta gtaaagagtg ggactggttt
     8761 agaaaccggg gagagattaa cagggccaga gccccatgtc tggtggtacc ggattgtaat
     8821 cctagcactt gagtgtggtc ttgataggag aatcaggagt tcaaggttag cttcaactat
     8881 agtgcgttcc aattccaggc caacaaggcg cataacgata ccacgatatc aacaagtttg
     8941 tacaaaaaag caggctggcg ccggaaccga agttcctatt ccgaagttcc tattctctag
     9001 aaagtatagg aacttcgaac cctttcccac accaccctcc acacttgccc caaacactgc
     9061 caactatgta ggaggaaggg gttgggacta acagaagaac ccgttgtggg gaagctgttg
     9121 ggagggtcac tttatgttct tgcccaaggt cagttgggtg gcctgcttct gatgaggtgg
     9181 tcccaaggtc tggggtagaa ggtgagaggg acaggccacc aaggtcagcc ccccccccct
     9241 atcccatagg agccaggtcc ctctcctgga caggaagact gaaggggaga tgccagagac
     9301 tcagtgaagc ctggggtacc ctattggagt ccttcaagga aacaaacttg gcctcaccag
     9361 gcctcagcct tggctcctcc tgggaactct actgcccttg ggatcccctt gtagttgtgg
     9421 gttacatagg aagggggacg ggattcccct tgactggcta gcctactctt ttcttcagtc
     9481 ttctccatct cctctcacct gtctctcgac cctttcccta ggatagactt ggaaaaagat
     9541 aaggggagaa aacaaatgca aacgaggcca gaaagatttt ggctgggcat tccttccgct
     9601 agcttttatt gggatcccct agtttgtgat aggcctttta gctacatctg ccaatccatc
     9661 tcattttcac acacacacac accactttcc ttctggtcag tgggcacatg tccagcctca
     9721 agtttatatc accaccccca atgcccaaca cttgtatggc cttgggcggg tcatcccccc
     9781 ccccaccccc agtatctgca acctcaagct agcttgggtg cgttggttgt ggataagtag
     9841 ctagactcca gcaaccagta acctctgccc tttctcctcc atgacaacca ggctcccagg
     9901 tcccgaaaac caaagaagaa gaacgcagat cgcagatcgc agatctctag agtcaccgag
     9961 ttgctttacc ggatgaagag ggccgaaaca tactgtccaa ggcccttgct ggcaatccac
    10021 ccaactgaag ccagacacaa acagaaaatt gtggcaccgg tgaaacagac tttgaatttt
    10081 gaccttctca agttggcggg agacgtcgag tccaaccccg gaccggtgca tgtggacccc
    10141 aagaaaaagc ggaaggtgga ccctaaaaag aaacgcaaag tggatcctaa gaaaaaaaga
    10201 aaggtgcacg tgtccatggt gtccaagggc gaggaactgt tcaccggcgt ggtgcccatc
    10261 ctggtggaac tggatggcga cgtgaacggc cacaagttca gcgtgtccgg cgagggcgaa
    10321 ggcgacgcca catacggaaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
    10381 gtgccttggc ctaccctcgt gaccacactg acctacggcg tgcagtgctt cagcagatac
    10441 cccgaccata tgaagcagca cgacttcttc aagagcgcca tgcccgaggg ctacgtgcag
    10501 gaaagaacca tcttctttaa ggacgacggc aactacaaga ccagggccga agtgaagttc
    10561 gagggcgaca ccctcgtgaa cagaatcgag ctgaagggca tcgacttcaa agaggacggc
    10621 aacatcctgg gccacaagct ggagtacaac tacaacagcc acaacgtgta catcatggcc
    10681 gacaagcaga aaaacggcat caaagtgaac ttcaagatcc ggcacaacat cgaggacggc
    10741 tccgtgcagc tggccgacca ctaccagcag aacaccccta tcggcgacgg ccctgtgctg
    10801 ctgcctgaca accactacct gagcacccag agcgccctga gcaaggaccc caacgagaag
    10861 agggaccaca tggtgctgct ggaattcgtg accgccgctg gcatcaccct gggcatggac
    10921 gagctgtaca agcctgcagg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag
    10981 gagaatcccg ggccgtccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc
    11041 gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg
    11101 ttttctgagc atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc
    11161 aagttgaata accggaaatg gtttcccgca gaacctgaag atgttcgcga ttatcttcta
    11221 tatcttcagg cgcgcggtct ggcagtaaaa actatccagc aacatttggg ccagctaaac
    11281 atgcttcatc gtcggtctgg gctgccacga ccaagtgaca gcaatgctgt ttcactggtt
    11341 atgcggcgga tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg
    11401 ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg aaaatagcga ccgctgccag
    11461 gatataagga atctggcatt tctggggatt gcttataaca ccctgttacg tatagccgaa
    11521 attgccagga tcagggttaa agatatctca cgtactgacg gtgggagaat gttaatccat
    11581 attggcagaa cgaaaacgct ggttagcacc gcaggtgtag agaaggcact tagcctgggg
    11641 gtaactaaac tggtcgagcg atggatttcc gtctctggtg tagctgatga tccgaataac
    11701 tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc catctgccac cagccagcta
    11761 tcaactcgcg ccctggaagg gatttttgaa gcaactcatc gattgattta cggcgctaag
    11821 gatgactctg gtcagagata cctggcctgg tctggacaca gtgcccgtgt cggagccgcg
    11881 cgagatatgg cccgcgctgg agtttcaata ccggagatca tgcaagctgg tggctggacc
    11941 aatgtaaata ttgtcatgaa ctatatccgt aacctggata gtgaaacagg ggcaatggtg
    12001 cgcctgctgg aagatggcga tctcgagcca tctgctggag acatgagagc tgccaacctt
    12061 tggccaagcc cgctcatgat caaacgctct aagaagaaca gcctggcctt gtccctgacg
    12121 gccgaccaga tggtcagtgc cttgttggat gctgagcccc ccatactcta ttccgagtat
    12181 gatcctacca gacccttcag tgaagcttcg atgatgggct tactgaccaa cctggcagac
    12241 agggagctgg ttcacatgat caactgggcg aagagggtgc caggctttgt ggatttgacc
    12301 ctccatgatc aggtccacct tctagaatgt gcctggctag agatcctgat gattggtctc
    12361 gtctggcgct ccatggagca cccagtgaag ctactgtttg ctcctaactt gctcttggac
    12421 aggaaccagg gaaaatgtgt agagggcatg gtggagatct tcgacatgct gctggctaca
    12481 tcatctcggt tccgcatgat gaatctgcag ggagaggagt ttgtgtgcct caaatctatt
    12541 attttgctta attctggagt gtacacattt ctgtccagca ccctgaagtc tctggaagag
    12601 aaggaccata tccaccgagt cctggacaag atcacagaca ctttgatcca cctgatggcc
    12661 aaggcaggcc tgaccctgca gcagcagcac cagcggctgg cccagctcct cctcatcctc
    12721 tcccacatca ggcacatgag taacaaaggc atggagcatc tgtacagcat gaagtgcaag
    12781 aacgtggtgc ccctctatga cctgctgctg gaggcggcgg acgcccaccg cctacatgcg
    12841 cccactagcc gtggaggggc atccgtggag gagacggacc aaagccactt ggccactgcg
    12901 ggctctactt catcgcattc cttgcaaaag tattacatca cgggggaggc agagggtttc
    12961 cctgccacag cttgatgaag atctgagctc ttaattaatg atcataatca gccatatcac
    13021 atctgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
    13081 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
    13141 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    13201 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgtttaaact aactttaaat
    13261 aattggcatt atttaaagtt acatatggcg gccgccctaa ggctcgacct cgaaattcta
    13321 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
    13381 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
    13441 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
    13501 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
    13561 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
    13621 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
    13681 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
    13741 ttccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
    13801 ttcctcatct ccgggccttt cgacctgcat ccatctagat ctcgagcagc tgaagcttac
    13861 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
    13921 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccaga
    13981 tcgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
    14041 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
    14101 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
    14161 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
    14221 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
    14281 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
    14341 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
    14401 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
    14461 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcgat
    14521 gatatcagat ccccgggatg cagaaattga tgatctatta aacaataaag atgtccacta
    14581 aaatggaagt ttttcctgtc atactttgtt aagaagggtg agaacagagt acctacattt
    14641 tgaatggaag gattggagct acgggggtgg gggtggggtg ggattagata aatgcntgct
    14701 ctttactgaa ggctctttac tattgcttta tgataatgtt tcatagttgg atatcataat
    14761 ttaaacaagc aaaaccaaat taagggccag ctcattcctc ccactcatga tctatagatc
    14821 tatagatctc tcgtgggatc attgtttttc tcttgattcc cactttgtgg ttctaagtac
    14881 tgtggtttcc aaatgtgtca gtttcatagc ctgaagaacg agatcagcag cctctgttcc
    14941 acatacactt cattctcagt attgttttgc caagttctaa ttccatcaga agctggtcga
    15001 gtaactttaa ataattggca ttatttaaag ttaggcgcgc cataacttcg tatagcatac
    15061 attatacgaa gttatgtcga gatatttaaa tattagaccc agctttcttg tacaaagtgg
    15121 ttgatatctc tatagtcgca gtaggcggtt ttagtctcag cccttaggat acacaggcaa
    15181 gtggttagtc cagtgatacc ctgtctcaac aataacaacg acagaggatt ctaacttctt
    15241 attactctaa tatcacttaa ggtcagaatt cagaggtaat tttacagtcc ctgaaaaggc
    15301 tgtcttgcgt caaatagctt tgcagctgtg ccaagcactt ggcattaggt gagaaggata
    15361 ttcaaactag ataaacgaaa aacaggtaag ataaactaga gacagactca cgggggaagg
    15421 ggagccaagc ctaggctggc tccttgcaca caactgaatt tgctttcatc ttctgccgtt
    15481 ctacactgac cccaccctct ctcttcttct ttacagcttt tgcagacagg cagagtggta
    15541 agtctgagct tcccttgttt acaacagaac gctatgctca ccttgtaggg aaggtctgtt
    15601 ttaccaaggg tcttgcctgg gttcagatgg agcagttctc tcacaatggg ccctggagta
    15661 taatccttgc taagactcca actgggttct ccaaagacag aaaggaaaga gactagggca
    15721 agcatgcttt tttttttttt taaatggttt agagattgaa cctagataga acctggagtg
    15781 tgccacacaa gcttctaccg ctgagttaca agtcttttaa aatttatttt ggatagaatt
    15841 gctaagttgc ccacagtggt cttgaattgt tatatatcac aatcacagtc agatttttat
    15901 tttattttat tttattttat tttattttat tttattttat tttattttat tttattttat
    15961 tttattaaga cagggtttcg gccgggcgtg gccaccactg cccagtacag tcagatttta
    16021 aatgctccac ccttcccatt gctagatttc tgctgtgaat cattaccctc agacctttaa
    16081 ctggtctgcg atggtggact ctctggcccc ttgcacctct ttggaatgca cagtccctca
    16141 aagttgagtt gttcatatct tctctcactc tcagcagctc ttccgaaggc tgtggtgaaa
    16201 ctggaccccc catggatcca ggtgctcaag gaagacatgg tgacactgat gtgcgaaggg
    16261 acccacaacc ctgggaactc ttctactcag tggttccaca actggagttc catccggagc
    16321 caggtccaat ccagctacac gtttaaggcc acagtcaatg acagtggaga atatcggtgt
    16381 caaatggagc agacccgcct cagcgaccct gtagatctgg gagtgatttc tggtaagtaa
    16441 aggctggccc cgggaggaga tgggatacga ggactggaga atctgcttac agggaagaag
    16501 tttacaagaa aaaggatggt ctggtttctg ggaagcatca tggagagctt ttctgaaaga
    16561 cattggtcct ctcatttccc ttttcgctta ctctgtgtga gatggcgcaa cgcaattaat
    16621 gataacttcg tatagcatac attatacgaa gttatggtct gagctcgcca tcagttcagg
    16681 cttctgccag agaagcagaa ggaaggacct gctttgtaaa catcagctct gccagggcag
    16741 ttgggggtgg gggtggggag cacagagtct agacaggtat actctatagg agaggggcag
    16801 cctccacaag gaggcagaag cagtcgaatc tctgagttcg aggccagcct ggtctacaga
    16861 gtgagttcca ggacagccgg ggctatacag agaaaccctg tctcgaaaaa acaaacaaat
    16921 aaaacaaaaa gtcgtcacac agcctatctg ttttctacac gttggactgg attcccttcc
    16981 cctcattgct ggcctcacat gccagatttc agttccaccc ttggagatct tcatatcacc
    17041 acatctgact tttcagtaag atggtttttt tcgagacagg gtttctctgt gtagccctgg
    17101 ctacacagaa atccccctgc ctctgcctcc cgagtgctgg gattaaaggc gtgcgccacc
    17161 acgcccggct ggtttttttt tgtttttgtt tgtttgtttg tttgtttttt taagatttat
    17221 tatgtatata acattctact tacatttagg cctgaaggcc agaagagggc gtccgatctt
    17281 attatagatg gttgtgaacc accatgttgt tgctgggaac tgaactcagg acctctggaa
    17341 gagcagatgg tactcttaac ctctgagcca tctctcccgc ccccatgagg ttcttaaata
    17401 catgaggtca tttaattaca gagctccgga ccgacatgca tcctacacag gagataacgg
    17461 atgtggccac gaggcttgaa agctagggtt ttttttatgg ggtaaggggc ttagggatgt
    17521 gaaatttagc atagcaacac actattggct tattcaaaca tcagcggaaa aattacatgt
    17581 acgtgcaggg tgtcagagtt ctgtgagttg ttgactcagt tcagatagcc ctgttatgtc
    17641 ctcacagtcc cctttatctt atggtcaaga tgttctcagg aatgttttaa ctacggggca
    17701 tacccaggtt tgtttttctt ttttctcagc cccaggcagc ctctaggctc acaaccaacc
    17761 agcaatttct gagtacttta tatgacttac aggtcttgaa gtttcatctt tcttccacta
    17821 gcatgaatgc cttctttata tttcacggaa catctagcca ttttgttcag attcacaaga
    17881 gaacacagaa tcaaaaaaga tctcaccagc caagcagtgg tagctcatgc ctttaatccc
    17941 agcacttagg aggcagaagc agtcgaatct ctgagttcga ggccagcctg gtctacagag
    18001 tgagtgggac aaccagagct actataaaga gacccaacac atctcaaacc aagccaaacc
    18061 aaaccaacca accatatagc tcacacctgt atgtaatatc aactgaggca cggagcttgc
    18121 tgctggcctt agttacatga tgagttcttg gccagcctga gctacagtcc cctcaataag
    18181 tcatacattt cctttttgtt tgtttgtttg tttgtttgtt tggtggtgga agattccact
    18241 aaggtgtgtc atgccctcaa atgcttatga tccaagactg agatactgta atcttaacac
    18301 caagagaccg tattaggggg tggtattcag aggaggtaac caggtcatag ttggagtcca
    18361 tatgagggag acctacaaaa gttctgtagg aatgacaata aagctcactt ctataaacta
    18421 ggaggtaagc tctcattcaa tgtacacatt tctggtctct cactttagag taatttgaga
    18481 agtaagtgtg cattgtttat aagccactaa ggttccgttt tgagcatttg tttattaaga
    18541 ttgagtacca cgaagcccag gtttgagggt gaccttgaac tcttgatcct tctgctccac
    18601 cttccacgtt ctgggattac agacacgttc taccacatct ggctcctgag tgcttagttc
    18661 atggcacttt gtcaaagcag cctgagtaga gcagggcaga gcctggcctg attcaggctc
    18721 ctctcactca aacacagttc atgactcctt gcctcacttc ctaaaatttg ttcaaaaatc
    18781 atatcattaa attagtaatc ctaaaccata tgcaacactt atgctaataa accgaaggct
    18841 ctattctata actcatatag ttactgaagt taatatatgt tccacattta gtgttattgg
    18901 tttgtttgtt tatttgagac agggtttctc tgtgtggcct tggctgtcct ggaactcact
    18961 ctttgaactc agaaatccac ctgcctctgc ctccggagtg ctgggattaa aggcgtgcgc
    19021 cacccctgcc cggccattta gtattttaaa tgcataaaac tttttcaaaa ttgcttttaa
    19081 tacctcatag atttaatggc atgtttttcc tgtgtgagtc ttgtagccct gtatgatagg
    19141 cagttgtaga ggccatggca tatatgtata tatttgtgga ttttgttttt tttttttgtt
    19201 tttttttggt tttttttttg tttttttgtt tgtttttttt tgtttgtttg ttttttgttt
    19261 tttgtttttg ttttttcgag acagggtttc tctatagccc tggctgtcct ggaactcact
    19321 ttgtagacca ggctggcctc gaactcagaa atccacctgc ctctgcctcc cgttgttttt
    19381 gttttttaag atttacttat taaatgtata tgagtacaca atagttgtcc agacacacca
    19441 gaagagggca tccgatttca ttacagatgg ttgtgagcca ccatgtggtt ggtgggaatt
    19501 gaactcagga cctctggaag agcagtcagt gctcttaacc actgagccat ctctccagcc
    19561 ctggctgttg agatagggtt catatggtcc aggtttgcta tatagcagat gaccttgaat
    19621 ttctgattgt tctgcctcca tctctgaggt gttgtgattt tacaacattc cctgtcccta
    19681 gggattgaac ccgggccctt atgtattcat tctgctgaga gatagctaca gcacttgggt
    19741 atctttttgt ttgtgtgttg gttggttttt tgcaacaggg tttctctgtg tagtccctgc
    19801 tggaactcct ctgtagacca ggctggcctg gaactcacag agctccacct gcctctgtct
    19861 ccagagtgct gggattaaag gtgtgtgaca ctatggccca gctacccctt tgtatcttac
    19921 tcttggtaaa ttttctttat gtctttcaga ctggctgctg ctccagaccc ctcagcgggt
    19981 gtttctggaa ggggaaacca tcacgctaag gtgccatagc tggaggaaca aactactgaa
    20041 caggatctcg ttcttccata atgaaaaatc cgtgaggtat catcactaca aaagtaattt
    20101 ctctatccca aaagccaacc acagtcacag tggggactac tactgcaaag gaagtctagg
    20161 aagtacacag caccagtcca agcctgtcac catcactgtc caaggtgagc tgctatgcct
    20221 ggctttaaaa catattactt tatttcaaaa cattctgtct ggtgtcctaa tgctaggatc
    20281 ct
//