LOCUS       KO-first_condition_ready_122469_MGI:3039603        22495 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector B3gnt6 targeting project(s): 122469
ACCESSION   unknown
SOURCE      Mus musculus.
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GTK_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 114286
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            5377..5412
                     /db_xref="ENSEMBL:ENSMUSE00000633521"
                     /note="ENSMUSE00000633521"
     misc_feature    4275..9728
                     /note="5 arm"
     misc_feature    9756..9780
                     /note="B1 Gateway"
     misc_feature    9793..9840
                     /note="Frt"
     misc_feature    9841..10341
                     /note="mouse En2 intron"
     primer          complement(9909..9930)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9805..9828)
                     /note="FRT3"
     primer          13844..13862
                     /note="seq primer NF"
     misc_feature    13904..13911
                     /note="RE sites"
     misc_feature    15704..15737
                     /note="loxP"
     misc_feature    complement(13912..13943)
                     /note="Rox"
     misc_feature    complement(15664..15695)
                     /note="Rox"
     misc_feature    13944..13965
                     /note="polylinker"
     CDS             14524..15120
                     /note="puromycin N-acetyl transferase"
     misc_feature    14639..14644
                     /note="Mutated RsrII site"
     misc_feature    10878..11594
                     /note="EGFP"
     misc_feature    10788..10877
                     /note="3X NLS"
     misc_feature    13660..13898
                     /note="SV40 polyadenylation site"
     misc_feature    12693..13637
                     /note="ERT2"
     promoter        13976..14487
                     /note="PGK promoter"
     misc_feature    15231..15663
                     /note="PGK pA"
     primer          13718..13741
                     /note="LRPCR RAF5"
     misc_feature    11604..11657
                     /note="T2A"
     misc_feature    11654..11654
                     /note="A to G to kill RsRII site"
     misc_feature    11658..12683
                     /note="Cre"
     misc_feature    10616..10637
                     /note="seq primer FCHK2"
     misc_feature    10342..10778
                     /note="mouse En2 exon"
     misc_feature    10730..10798
                     /note="FCHK cre_ert_gtk"
     misc_feature    10730..10765
                     /note="FCHK QC prefix region"
     misc_feature    10766..10778
                     /note="FCHK QC critical region"
     misc_feature    10779..10798
                     /note="FCHK QC suffix region"
     misc_feature    15760..15780
                     /note="B2 Gateway"
     misc_feature    9729..15810
                     /note="Synthetic Cassette"
     exon            16166..18497
                     /db_xref="ENSEMBL:ENSMUSE00000633520"
                     /type="targeted"
                     /note="target exon 1 ENSMUSE00000633520"
     misc_feature    15811..18654
                     /note="Critical Region"
     misc_feature    18678..18711
                     /note="Downstream LoxP"
     misc_feature    18655..18734
                     /note="synthetic loxP region"
     misc_feature    18735..22495
                     /note="3 arm"
BASE COUNT     5766 a   5723 c   5593 g   5412 t     1 others
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaagtggg tgcagtataa gaaacttgag tcaaaccaca aaatattgag
     4321 aaatactggc tgtgactgtc tgcattaagc tctccaccag acaaataaca tgtgaagttt
     4381 tctgggctca ctccagtgcc cttcctctag cttcctctgc cattccactc atgtcctata
     4441 atctcattta cattttacat tttacatatg atttccatct cccaagacat gctgagttcc
     4501 ttgagggcta ggactatgtc taattcatat ctgtcctgag tactctcaga agcactgaga
     4561 taggctcttc accacttgcc aaattcagag tactcagggc tgggtgaaac ttttcaaaga
     4621 atcaagtgaa aactagagct agggagagga agctcagtgg tatgccacat atttggtatg
     4681 cacaaggccc taggttctag tacccctgct ctcaaaatca gtatactcta atgtcatttt
     4741 tatgtatgtg tctaggtcta tgtgcagacc agaggtcaaa atcaagtgtc tttctctatc
     4801 actttccctc ttaattatgc gacaaggtct ctcaccatta acctggagtt ggccagcaag
     4861 gccccaatta ctcttgtctc tgtcttccca gagctgggat ttcagatatg caccactatg
     4921 cctggctttc tacaaggatt ctaggagtca aaacgcaggc cttcatgttt acatagcaag
     4981 cactttacct actgagctag ttcccaagcc tctatatgca acttataaag attttaaaga
     5041 atagcctgtg ttagcagcag cttctatacc cggagtggtt ctaatctagt agggaactcc
     5101 aattgtgaga gaaagcatta agattgtttg ttcatcctac cagcaaaagc agggtagacg
     5161 ggttaaagaa tagtttccca gaggaggcaa cattctagct tgatctgaaa atgcaagcag
     5221 ttagttgcac aggctaagaa agcttattgc taccttcacc ctacccctga ggaggaggag
     5281 tgatggcctg gattggtcaa atgcaagaat actaaactaa gccactctca caggggtgga
     5341 acccagtgtg tagcaagggt tgtcctggtc tgggatggca cgggccagat tctcctctct
     5401 caaacgcaca aggtaagatg gggtgggcgg gttcttctta ttgagaaaga atgtatttat
     5461 ttgtgtatga gacagaaaga aagatggggg ggggggcaag aaaggaggaa ggggagtaga
     5521 ggggggaggg agagagagaa caaacatgga ggtcagagac aacttgcaaa gttctaccat
     5581 gtaagtcctg gcaacagcag ggccatctac tgaaccacct tgccaaacct cactcagggt
     5641 tattcttttg gggtattatt ttaccctcct ctcactccac tcccttctta gagaagaaaa
     5701 ctgcacagtc catgcagccc caagaggttc atggagtgct gggaagaggc cagacaccaa
     5761 gaggtagcta aagttatttt gcctgtcatg tcttctattc aaatactact gcccgggcac
     5821 cccctcccct gctggaacac ttcggcatgc tgctacagga atggggtggc cctgccatcc
     5881 gagcttcaaa ccaaaggagg aaatagccaa ggtagttctt aagaagaaag aggataaatt
     5941 cagagcatag agaaccttgt tacaagtgta gagcaataag cagtgggctg cagcccaaag
     6001 accacaaagc gcctgtagaa ggatggagcc cttcagagga tggctagcct ggctccataa
     6061 gggcgtgcct attagcacta aggttacgga ggctcttaac ccatggcaga catgctttgc
     6121 atcaactcac agcatgagcg actgagacaa cctgcccaat ctcacagaca gactgccctc
     6181 tttcatccaa ggtcagtagc cacagcacca aggagacaga tctgttgacc tggacttacc
     6241 aggaggagac ctgatggagc tagtaccgga gctgaattca agctgagaca agggagcagg
     6301 gtctgtggag accagcagag ctttaggttg gggctccagg cacctagaag agtagtttga
     6361 gaaaagggag gtggagcaaa aaggaccaaa gagaagacac ttggtttcaa gttagagcca
     6421 agtcagaggc tgtacatctt aagcacagag cgagccagct ccgccctcag aaagggctat
     6481 gcccttcccc acatcatttc cctcctttta tactgaaagt tggccatgct ccagggtctt
     6541 cagacttatc aaaataaatg ctctcttctt tgaaaccaaa tctacggatg cattaagatt
     6601 gagaaagctt ttctttctct aaaccaccac agctggttta gagagatgct tttaaattat
     6661 gttttaagat gagaaggaga gtagatgaag gcaggagatc agaagttcaa aactagccta
     6721 agccatataa aaacctgacc aagcctgctc agtcggtcaa caggattttt agcatgagaa
     6781 tgagggttaa aaagaaaaaa gacagttaga taacattgta tagcatgacc ccagtcaatt
     6841 ctgagactga agtcaaattt aattttccca atccgctttt tgtaatattt taattacatg
     6901 cacgtattaa gggctagtag tacattgagg aatcaagcaa gacaatagtc atcaaagccc
     6961 catgatcact ctggtgatag ctgtttctaa agatgatcaa gatatctgag cctacttctt
     7021 cattctaccc taaaaccaga ttcttacttc cttgtccttg gccaaggtca ggtccctgcc
     7081 agaacttatg tctttgtcat gctctaatgt cagattcctg ccaagcaacc catttcccta
     7141 tccttggtca atgtcagatt cctgaaaaat ggcaccccaa aagactctca aaaaaaaaac
     7201 ctgtctttaa aaagctaaaa taaccagggc ttggcggtgc aagccttaaa tcccagctct
     7261 ttgtgagttt taggccagct ttgtttacat agtgagtttc aggccagcca gtctcaaaaa
     7321 ataaaaataa acctaaaata taacagttta atcaccattt ataaggcatt tatcatttgt
     7381 atgatcactg agagatgtta tgtttttttc tgaggggaaa ctcaaaccca gggggttaac
     7441 tcaagtctca agcccagcta ttcagactcc aaatcctata ttcattcata ttttattctt
     7501 tttattcact gaaaaagtct ttataggctg ccagtgtagc tctgttggta gagtgctagc
     7561 ccatcatata tgaaggaagc agtgggttct attcccaata ccacatactc tgggcatggt
     7621 gctatatacc ttaattccag cacctggaag gtagaggcag ggggatcaga agttcaaggt
     7681 tatccttgga tatgagagtt tgaaggccag gctggagtac ttaagacctg gtcagaagaa
     7741 atgggggagg tggagggaag gaaggaagga aggaaggaag gaaggaagga aggaaaaaag
     7801 aagggaagga tagaggagca agagtagaag cattggagag gggagaaagg gagaaagaaa
     7861 ggaaattgaa ataaaaacac tgacccaaat ttgctagaca caggcgtatg cactgagtaa
     7921 ctgacagtga cattggcatc atgtgtcact gtcctcctct aatgcaccag caccgaggca
     7981 ggactgagca acagtggaaa tctacagctg gctttcagcc gtgccactgt gtacacacag
     8041 ttctctactt gtcgggcgac taactgctac cactcagccc tgagcacccg tttggtgctc
     8101 attccgagct ggacactgta tggactgtaa tacacaagtg ctaggacaca gtcccttcca
     8161 gtaacaccca gcttcacaag ctactctctg aaaacagagg acgagcacat gttctagaac
     8221 attctttagc acctgacagt cctactagct aacatatact aatttagcat tactgacttg
     8281 ccaagtacta tattattatt ttcattttgg tttttaaaac ttatttactg ttgtcactat
     8341 tgtttaaggt tatctgtgat atatatatgc catagcatag atgtcaaggc ctgagggaag
     8401 tctctgggag tccatgtgga tcacagggag ctaactcagg tcactgggct tagtggcaag
     8461 caccttactc cactaagtgc cttcactggc cctctttgag tttcactata cagcctaggc
     8521 tggctctgaa ctcacatcct gctcagcctc cttgagtcag ggattataga catgagcttc
     8581 catccctggc ttctgtactg aattctctga acatattact atttacttac cactaaaagt
     8641 cctaagaggg ccaggtaagt aactctggca ctgcacttgc tcagtgcgtg caagagctgt
     8701 ggtttgattc cttgtggcat atacccttaa tttcagcact tgcttggcag gctgagaccc
     8761 gaagatccag agtctgaaac tactctagac tacagagtga ggccatgtct taaaagttaa
     8821 aaaacaagca agcaaacaaa aaccttaggt atgaaaacaa gcctatgtct gcttgcttaa
     8881 cccatgcagc tggaaagtag cagaagtaag atttgaacct gggtaaacag ctttcaccac
     8941 actgttccct caggctacaa tggtgaagaa tagattccat ccctgcccca gatgtgctgg
     9001 tggactagat ggggaggcag aggggaatga ttgacagttt gcctgccctc ctttgactgc
     9061 cagtgaagct ttctcagggt ttcacctctt ccaagtgtac cacaagaagg gtaataaagg
     9121 caggtgagat cagtgcaggt tagcaaacgg cacaggacaa aattccaggg caggaagagg
     9181 catttcagat ggccaggtga gagagcaaag ttcaaagttt actcttaccg cctcaaacag
     9241 ggagtggccg ctggccgttc ctgtaggaaa gtgtcttagt caatgttatc attgctgtga
     9301 tgaaacatca tgaccaaaag caacttgggg aagaaagggt ttggcttaca tactattgtc
     9361 ctctcagcct gtgctggatc cagctggaca gagatggaga ggaaaggata taccagcgag
     9421 aaatgtagaa tgcagacatc aacagattgt ggggaggaga tactatttga gcaaaagggg
     9481 gaatagtggt accatggaca gagaaaggct gggcggaagc tcgccgcaag gcagggactg
     9541 actctgaaca cgtgtctgtc atcctgggta acatctaggg gtatccatta gccatgatac
     9601 acacagctgt gaaggtgaac acactctcag cttgggagac ctaagacgtt cactgtcctc
     9661 aggaatttgg accccacctc acccccattc cttattctgt tttagttcag tttattctcc
     9721 tgccgcagaa ggcgcataac gataccacga tatcaacaag tttgtacaaa aaagcaggct
     9781 ggcgccggaa ccgaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
     9841 gaaccctttc ccacaccacc ctccacactt gccccaaaca ctgccaacta tgtaggagga
     9901 aggggttggg actaacagaa gaacccgttg tggggaagct gttgggaggg tcactttatg
     9961 ttcttgccca aggtcagttg ggtggcctgc ttctgatgag gtggtcccaa ggtctggggt
    10021 agaaggtgag agggacaggc caccaaggtc agcccccccc ccctatccca taggagccag
    10081 gtccctctcc tggacaggaa gactgaaggg gagatgccag agactcagtg aagcctgggg
    10141 taccctattg gagtccttca aggaaacaaa cttggcctca ccaggcctca gccttggctc
    10201 ctcctgggaa ctctactgcc cttgggatcc ccttgtagtt gtgggttaca taggaagggg
    10261 gacgggattc cccttgactg gctagcctac tcttttcttc agtcttctcc atctcctctc
    10321 acctgtctct cgaccctttc cctaggatag acttggaaaa agataagggg agaaaacaaa
    10381 tgcaaacgag gccagaaaga ttttggctgg gcattccttc cgctagcttt tattgggatc
    10441 ccctagtttg tgataggcct tttagctaca tctgccaatc catctcattt tcacacacac
    10501 acacaccact ttccttctgg tcagtgggca catgtccagc ctcaagttta tatcaccacc
    10561 cccaatgccc aacacttgta tggccttggg cgggtcatcc ccccccccac ccccagtatc
    10621 tgcaacctca agctagcttg ggtgcgttgg ttgtggataa gtagctagac tccagcaacc
    10681 agtaacctct gccctttctc ctccatgaca accaggtccc aggtcccgaa aaccaaagaa
    10741 gaagaacgca gatcataact gactaggagg ccaccatggg cggaccggtg catgtggacc
    10801 ccaagaaaaa gcggaaggtg gaccctaaaa agaaacgcaa agtggatcct aagaaaaaaa
    10861 gaaaggtgca cgtgtccatg gtgtccaagg gcgaggaact gttcaccggc gtggtgccca
    10921 tcctggtgga actggatggc gacgtgaacg gccacaagtt cagcgtgtcc ggcgagggcg
    10981 aaggcgacgc cacatacgga aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
    11041 ccgtgccttg gcctaccctc gtgaccacac tgacctacgg cgtgcagtgc ttcagcagat
    11101 accccgacca tatgaagcag cacgacttct tcaagagcgc catgcccgag ggctacgtgc
    11161 aggaaagaac catcttcttt aaggacgacg gcaactacaa gaccagggcc gaagtgaagt
    11221 tcgagggcga caccctcgtg aacagaatcg agctgaaggg catcgacttc aaagaggacg
    11281 gcaacatcct gggccacaag ctggagtaca actacaacag ccacaacgtg tacatcatgg
    11341 ccgacaagca gaaaaacggc atcaaagtga acttcaagat ccggcacaac atcgaggacg
    11401 gctccgtgca gctggccgac cactaccagc agaacacccc tatcggcgac ggccctgtgc
    11461 tgctgcctga caaccactac ctgagcaccc agagcgccct gagcaaggac cccaacgaga
    11521 agagggacca catggtgctg ctggaattcg tgaccgccgc tggcatcacc ctgggcatgg
    11581 acgagctgta caagcctgca ggcgagggca gaggaagtct tctaacatgc ggtgacgtgg
    11641 aggagaatcc cgggccgtcc aatttactga ccgtacacca aaatttgcct gcattaccgg
    11701 tcgatgcaac gagtgatgag gttcgcaaga acctgatgga catgttcagg gatcgccagg
    11761 cgttttctga gcatacctgg aaaatgcttc tgtccgtttg ccggtcgtgg gcggcatggt
    11821 gcaagttgaa taaccggaaa tggtttcccg cagaacctga agatgttcgc gattatcttc
    11881 tatatcttca ggcgcgcggt ctggcagtaa aaactatcca gcaacatttg ggccagctaa
    11941 acatgcttca tcgtcggtct gggctgccac gaccaagtga cagcaatgct gtttcactgg
    12001 ttatgcggcg gatccgaaaa gaaaacgttg atgccggtga acgtgcaaaa caggctctag
    12061 cgttcgaacg cactgatttc gaccaggttc gttcactcat ggaaaatagc gaccgctgcc
    12121 aggatataag gaatctggca tttctgggga ttgcttataa caccctgtta cgtatagccg
    12181 aaattgccag gatcagggtt aaagatatct cacgtactga cggtgggaga atgttaatcc
    12241 atattggcag aacgaaaacg ctggttagca ccgcaggtgt agagaaggca cttagcctgg
    12301 gggtaactaa actggtcgag cgatggattt ccgtctctgg tgtagctgat gatccgaata
    12361 actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc gccatctgcc accagccagc
    12421 tatcaactcg cgccctggaa gggatttttg aagcaactca tcgattgatt tacggcgcta
    12481 aggatgactc tggtcagaga tacctggcct ggtctggaca cagtgcccgt gtcggagccg
    12541 cgcgagatat ggcccgcgct ggagtttcaa taccggagat catgcaagct ggtggctgga
    12601 ccaatgtaaa tattgtcatg aactatatcc gtaacctgga tagtgaaaca ggggcaatgg
    12661 tgcgcctgct ggaagatggc gatctcgagc catctgctgg agacatgaga gctgccaacc
    12721 tttggccaag cccgctcatg atcaaacgct ctaagaagaa cagcctggcc ttgtccctga
    12781 cggccgacca gatggtcagt gccttgttgg atgctgagcc ccccatactc tattccgagt
    12841 atgatcctac cagacccttc agtgaagctt cgatgatggg cttactgacc aacctggcag
    12901 acagggagct ggttcacatg atcaactggg cgaagagggt gccaggcttt gtggatttga
    12961 ccctccatga tcaggtccac cttctagaat gtgcctggct agagatcctg atgattggtc
    13021 tcgtctggcg ctccatggag cacccagtga agctactgtt tgctcctaac ttgctcttgg
    13081 acaggaacca gggaaaatgt gtagagggca tggtggagat cttcgacatg ctgctggcta
    13141 catcatctcg gttccgcatg atgaatctgc agggagagga gtttgtgtgc ctcaaatcta
    13201 ttattttgct taattctgga gtgtacacat ttctgtccag caccctgaag tctctggaag
    13261 agaaggacca tatccaccga gtcctggaca agatcacaga cactttgatc cacctgatgg
    13321 ccaaggcagg cctgaccctg cagcagcagc accagcggct ggcccagctc ctcctcatcc
    13381 tctcccacat caggcacatg agtaacaaag gcatggagca tctgtacagc atgaagtgca
    13441 agaacgtggt gcccctctat gacctgctgc tggaggcggc ggacgcccac cgcctacatg
    13501 cgcccactag ccgtggaggg gcatccgtgg aggagacgga ccaaagccac ttggccactg
    13561 cgggctctac ttcatcgcat tccttgcaaa agtattacat cacgggggag gcagagggtt
    13621 tccctgccac agcttgatga agatctgagc tcttaattaa tgatcataat cagccatatc
    13681 acatctgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
    13741 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
    13801 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    13861 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgtttaaa ctaactttaa
    13921 ataattggca ttatttaaag ttacatatgg cggccgccct aaggctcgac ctcgaaattc
    13981 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
    14041 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
    14101 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
    14161 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
    14221 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
    14281 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
    14341 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
    14401 tcttccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
    14461 tcttcctcat ctccgggcct ttcgacctgc atccatctag atctcgagca gctgaagctt
    14521 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
    14581 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatcca
    14641 gatcgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
    14701 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
    14761 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
    14821 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
    14881 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
    14941 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
    15001 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
    15061 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
    15121 tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg
    15181 atgatatcag atccccggga tgcagaaatt gatgatctat taaacaataa agatgtccac
    15241 taaaatggaa gtttttcctg tcatactttg ttaagaaggg tgagaacaga gtacctacat
    15301 tttgaatgga aggattggag ctacgggggt gggggtgggg tgggattaga taaatgcntg
    15361 ctctttactg aaggctcttt actattgctt tatgataatg tttcatagtt ggatatcata
    15421 atttaaacaa gcaaaaccaa attaagggcc agctcattcc tcccactcat gatctataga
    15481 tctatagatc tctcgtggga tcattgtttt tctcttgatt cccactttgt ggttctaagt
    15541 actgtggttt ccaaatgtgt cagtttcata gcctgaagaa cgagatcagc agcctctgtt
    15601 ccacatacac ttcattctca gtattgtttt gccaagttct aattccatca gaagctggtc
    15661 gagtaacttt aaataattgg cattatttaa agttaggcgc gccataactt cgtatagcat
    15721 acattatacg aagttatgtc gagatattta aatattagac ccagctttct tgtacaaagt
    15781 ggttgatatc tctatagtcg cagtaggcgg tatagtgtgg ggtacacaaa ccaggacctc
    15841 tttcatgcta gataatatta taccaacgga accaggccct gttgttttgt tttgttttgt
    15901 cttgttggga tttttggttg gttagtcggt tgggttatgg ttggttggtt agttggtttt
    15961 taaagcaagg tctcagtttg gccttgaact cactatgtat caaagcatga tctgaaatct
    16021 gaatcctcct gcctcttcct tcgttctcat gtgctaagag actacaggcc taattcagat
    16081 tctgggagag acctgatagg aataaggaag gattcctctg gtttaaaaga ctcatctatg
    16141 actcccctcc ccccaccccc cacagatggc tttgcccagc agccgcaggt tcaagagtcc
    16201 cacgacactg gctttctttc tagtgggtgt gactttagtg gtgctgaatc agtggttcct
    16261 ccaggaacac aggcaggaga aagccaaggg tcctgtggcc actcgccggt ctttggcagc
    16321 tgtggtacag cgttcgccgc tcttccaggt gcccccctgc gtggccaacg cctccgcgaa
    16381 cctcctgact ggcttccagc tgctgcctgc taggatccaa gacttcctgc gctaccgcca
    16441 ctgccgccgc tttccgcaac tgtgggacgc gccgcccaag tgcgcgggcc cccgcggcgt
    16501 cttcttgctg ctggccgtga agtcgtcgcc tgcgcactac gagcggcgcg agctgatccg
    16561 gcgcacgtgg ggccaggagc gcagctacag cggccggcag gtgcttcgcc tcttcctggt
    16621 ggggacctcc cctcccgagg aggcggctcg cgagcctcag ctggccgatc tgctgagcct
    16681 ggaggcgcgc gagtacggcg atgtgctgca gtgggacttc agtgacacct tcctcaacct
    16741 cacactcaag cacctgcacc tgctggactg gacagcggag cactgcccgg gcgtgagctt
    16801 cctgctcagc tgcgacgacg acgtcttcgt gcacacggcc aacgtactga gcttcctaga
    16861 ggtgcagtcg cccgagcacc acctcttcac cgggcagctc atggttggtt ctgtgcctgt
    16921 tcgcgaaagc gggagcaagt acttcgtgcc cccacagatc ttcccgggcg tagcctaccc
    16981 tgcgtactgc agcggcggcg gcttccttct gtcccgctac acagtccgca acctgcgcag
    17041 cgcggcacac cacgtcccgc tcttccccat cgacgatgcc tacatgggca tgtgtctgca
    17101 gcaggccggc ctggcgccca gcagccacca gggcatccgg cccttcggcg tgcagctgcc
    17161 caatgtgcag cgcttatcct tggatccctg catgtaccgc gagctgctgc tggtgcaccg
    17221 cttcgcgcct tatgagatgc tgctcatgtg gaaggcgctg cacaacccag cgctgcactg
    17281 tagccacaaa caggttgcag gatctcccac ggccggagaa cagaaccccg atgcacactg
    17341 atgtcccctt gaggacggat ctggaaacac atgttcctga cctcaacttc aggggttctg
    17401 gttctattgc tcaagagtgg cttcaaacat gtaggtggcg tttttcttgt tttaagccga
    17461 ctccaaggcc tctgggctgg ggctatctat ctgttcatcc tcacacagca gcctttcagg
    17521 gcatctgtct tctatcttcc attcttcgtg ggcagcaaga gaggcatggc ggccaagatc
    17581 aatgtcagct ttcaagtgca gcgctgttca gaggtcagtt tacattcaga actaaagggg
    17641 tgtctggcag cctcgcttct accatgctcc agggctcttg tgaagtcagt aaaaagcaaa
    17701 ggcccttaag ggcagcagag ggaagacctt cacagccatt tttctgtagc actgtgaagt
    17761 cccagtagct acagccttgc ctccccagtg ctttaggaag tgcacgtttg agtggacagt
    17821 ggccatctgt gagaacatta tattagtttc ctgttgctgc tataacaaat cttcattaac
    17881 tctcgaagcc taggaacaag cccgcgtatc ctgtagttct ccgacaacac aggtctcacc
    17941 accgggttaa tgtcaagatg ctggtaaaac tgctttcttt cctagtattc tatgggggag
    18001 aacccccccc cattccctag cttctagagg gagaggttct tcaccttcaa aaccagaaat
    18061 tgctacattt ccctacagcc aaatctctct ctgactcttc tttttcctcc atcctcaact
    18121 ctccttccct ggagttaaaa cctgggtcca cctgacagtc cagacttagc ttcccgtctg
    18181 tttgtggaga cagactcagg tagcccaggc tgccctgaaa ctttgtagtc aagcatcatc
    18241 ctgaattccg gaacctcctg ccttcacctc cccaagggtg gactgtagat ttgtctccat
    18301 gcccagctaa tctcttcatg ttgtaaagcc agatcactag gtatattatt gatcatcatg
    18361 tcgttcacat gtaacctaat ttagtcacag gtttcagaga tcagggtgtg gacatctttg
    18421 gaggaccatt ggttctgtct tccatgatgc cacacataaa tgtgtgagat aaacgataat
    18481 aaatgaagga aatgaacaca tgacaaacgt gctttcttct caacaatcag tcctgatgcc
    18541 agcgcttatc gaggacaatt gagcatcatt aattccgaat cccctgcttt gatgccttgt
    18601 tctcagttcc ctggcagaag gttgtctcca ttttgggggt aaaggtatta agatgagatg
    18661 gcgcaacgca attaatgata acttcgtata gcatacatta tacgaagtta tggtctgagc
    18721 tcgccatcag ttcagtgttt aggaaggtga gaaggcagca gatttaggtc tgctatgtaa
    18781 ctctggggcc tgctcactcg tctctgaggc tggattactc tagtgtccaa gaacaggaaa
    18841 ggccactggg tccagggaac cccagcacca cctgaagtca cctggaagaa caagcatatc
    18901 tttcaggttt ggccttctgg cccatgtgcc gccagggggc acacctgacc cacggtccgt
    18961 ctctccttgg tggcgctagg ctggacaact gggctgaggt gtccgggtgg ccaattccaa
    19021 gcggagtcca gaaaggcatg gggcaccagg ctttgttaga ctagtggctg ggagccggat
    19081 catacctggt atcacccgcc ctcactccgc acccagtacc ctcagggaaa ggccacactt
    19141 gggtgccccg actcctttga aaagcctaac ataatctgac ttcccaggaa cctaaggact
    19201 tggaaaaaga aagacgttct tgactcacga cgatagatta aaaaatccta cgtaagtatg
    19261 atatccccta gaagggtgtg gtgatacatg ccaaaacccc agccctcccg aggctgaggt
    19321 gcagggcccc gagttctaag cctgggctaa aactactaca agaccctgtt caaaaggaga
    19381 ggaaaaagac aagcctaggc ttccctggag ttcatgttga gttgagaaag gctttggaat
    19441 actgattttt tttttctgcc actggctccc aattcctggg gttataaatg agctccacca
    19501 taacccccaa aattattttt aaagtttcag aagttagggc tggaaagatg gctcagcagt
    19561 taaagacact tgagttcatt catccaaaca gcagttatac acaaataatg tgtgaggatg
    19621 ggggggaggg gggttgttgt gtcttagaag ggacaggaat ggtggttcca tgcttaagag
    19681 gttatacagc tataagacca gggttcagtt cccagaacct acatggtggc ttaaaaacca
    19741 tctagaattc ctgttcttgg atctaatgct gtcctctggt ttgggggatc actaaggtta
    19801 tgtgatgttc attactcact caggtacata cacaatttta attttgttaa gcctcggaag
    19861 tttacagcga aatcaaatta acaccctctt tttaaaatat gtatttatct aagacatgat
    19921 ctctcgaggc ccaggcttgc ccacaattgg cttcctagct gagaatggcc ccaaattcct
    19981 catctcctgc cctggaattc caagtgctgc ttttctaggt ctttctataa ggatttcacc
    20041 cttggaataa agttcttaca tgatgtaatc atacttttaa aaaaatcaat atattgctta
    20101 atttcatata aaatttaaac aaaagaaaaa cacattaaag taagtatagc ttgggcatat
    20161 caaatttttt tctctacata ttccaaaaca ttcctttagg gggcagaata ccttcttgag
    20221 aactgttaga ctgcacagag actcacacac agatagataa ttgattaaat tagtaagaaa
    20281 atacatcctt ttccagatgc aaaacaatta tgtaataaat gtagtttcga tatcaagaca
    20341 tcaagaaaag cttgctattg ctttgtggat gtccctttgt tttggtttca tttgtttatt
    20401 tcagtgtttt gaaacagggt cttgccctgt tgtccagact ggtcccaaac tcatgctttg
    20461 taagcccagt gctcaagctg aggcaggagg attgcctaaa gtctaggcca gtctgggtta
    20521 cagagcaagt ctccaaaaac aaaacaaaac aaaacattgt aaaacgtcag tataataaaa
    20581 tccaatgtac ttgggactga gttcaagaag acacagacaa aagcagtaat atgatttaag
    20641 atgtctttaa atatgtaaat taacgtctag agacagcatg tcttcaggta acagctaaat
    20701 ccctgattca ctggattctt ggcgccagaa acaggaacta tcagactgct gtggtatccg
    20761 tgcggtgcgg ctagggtgag gactgatgtc caggtagaga agagacttct tcaagatcac
    20821 aagggtctac agagcatcag ggactaagta ccccgtctcc tgcctgttcc tgagctctga
    20881 gccccaacac ctgctggttt ttcaccccct gaacagagag ctgcagaggc cagaggccag
    20941 cctgaagctg gcaggaggag acagacagca gtttggttgt ttgttatctt tatagtgtct
    21001 gctactggac tggccctccc ttcgccctat agggcttgag gaactgccaa tcttcagcat
    21061 taaatcaaca tcctaacaat gtcttccaga gaaaggcccc tcctccatcc tgcgacagtc
    21121 atttcaaact aatcctggct actgtaggct gcctgccccc agcttgcacc ccacccacct
    21181 gtgaatgaca aacagcttcc ctcagtgcaa aagagatctt tccagattga cctcagtgct
    21241 cccacagatg ctgatgtgct cccaaggatg cagaggacat cagttttcaa aatatccttg
    21301 ctctttattc ttgccctcaa gacttataag cagagtgaag actgagtgag attttcccat
    21361 ggcccttggc tgctggggca gggggctaag ctgaagcaaa aggaaggatg gagatagggg
    21421 ggagatggtg accaggaagc cccacctata gaaggaagaa gaggaggggt ctaagacccc
    21481 tcaggattgg gagggacttg gggccttgtc aggatgggtg ggaggaagtg tccttgggtt
    21541 tcaagtaagg taaaattaat tcctaaaagg tgggcataat gtgaggggag actctggaag
    21601 ctcatgaggg atatagaagt ccctgacaca cacatctgcc ccagagatgg gaacagttgc
    21661 cagtttgctt gggactccaa agagcaggat ccctgatacc agctcacctt tatgtcccag
    21721 ggacccagtg ttgtgaacta gttaatactt tatccagtgt ctttggcttt ccatttgtgg
    21781 gacttaaagg cttcctcttt gagtaagctt tactatccct aaccctcaag tgacaccaga
    21841 tccactgtca gccattaagg cccacgtgga taccctcggc accccataag ccacttgatt
    21901 ctagtactga gcaactcaca cagagcagtg tcagagtcca gactagagag acactgtgga
    21961 cgcttttgcc tgccaaagac agggacatgg ataggagtcc ccactccagt gtggcctatc
    22021 tcagcacttt gagaagcagt gtgtgagttc ttcaggagtt aaacatcact gccagggtgt
    22081 ggtgtacaca cctggtacac aattgccata ttagcacttg agaggctgag gcaggagaca
    22141 gagttcaagg tcagacagtg ctacacaggt ggactcttaa aataacaaat aagggggctg
    22201 aggagatggt tcagcggtta agacactgac tgtttttcca gaagtcctga gttcaattcc
    22261 cagcaaccat gtggtggctt aggctatcta taaagagatc tgatgcccct tcgggcatgc
    22321 aggtatacat ccatacagaa catttatgta tcaaaaaaaa aaaaaaaacc caaataaacc
    22381 aaataaaatc caagcaggtg tggcagcgca tagatgtaat cccaactgtt gggcggggaa
    22441 gcaggagagc cagagttcag tgcctctctc agctacatgg aaaatttaaa gttat
//