LOCUS       46084#pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20623 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 46084
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000042699
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..6424
                     /note="5 arm"
     misc_feature    6452..6476
                     /note="B1 Gateway"
     misc_feature    6489..6536
                     /note="Frt"
     misc_feature    6537..7036
                     /note="mouse En2 intron"
     primer          complement(6605..6626)
                     /note="LRPCR LAR3_EN2"
     primer          complement(6501..6524)
                     /note="FRT3"
     primer          10688..10706
                     /note="seq primer NF"
     misc_feature    10748..10755
                     /note="RE sites"
     misc_feature    12548..12581
                     /note="loxP"
     misc_feature    complement(10756..10787)
                     /note="Rox"
     misc_feature    complement(12508..12539)
                     /note="Rox"
     misc_feature    10788..10809
                     /note="polylinker"
     CDS             11368..11964
                     /note="puromycin N-acetyl transferase"
     misc_feature    11483..11488
                     /note="Mutated RsrII site"
     intron          7037..7411
                     /note="mouse En2 intron"
     misc_feature    7409..7411
                     /note="Splice Acceptor"
     exon            7412..7451
                     /note="mouse En2 exon"
     primer          7312..7333
                     /note="seq primer FCHK2"
     misc_feature    7452..7574
                     /note="1Dleader"
     misc_feature    7575..7626
                     /note="F2A"
     misc_feature    7722..8438
                     /note="EGFP"
     misc_feature    7632..7721
                     /note="3X NLS"
     misc_feature    10504..10742
                     /note="SV40 polyadenylation site"
     misc_feature    9537..10481
                     /note="ERT2"
     promoter        10820..11331
                     /note="PGK promoter"
     misc_feature    12075..12507
                     /note="PGK pA"
     primer          10562..10585
                     /note="LRPCR RAF5"
     misc_feature    8448..8501
                     /note="T2A"
     misc_feature    8498..8498
                     /note=" A to G to kill RsRII site"
     misc_feature    8502..9527
                     /note="Cre"
     misc_feature    7427..7452
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    7427..7442
                     /note="FCHK QC prefix region"
     misc_feature    7443..7446
                     /note="FCHK QC critical region"
     misc_feature    7447..7452
                     /note="FCHK QC suffix region"
     misc_feature    12604..12624
                     /note="B2 Gateway"
     misc_feature    6425..12654
                     /note="Synthetic Cassette"
     exon            13014..13161
                     /db_xref="ENSEMBL:ENSMUSE00000421185"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000421185"
     misc_feature    12655..13331
                     /note="Critical Region"
     misc_feature    13355..13388
                     /note="Downstream LoxP"
     misc_feature    13332..13411
                     /note="synthetic loxP region"
     exon            14462..14617
                     /db_xref="ENSEMBL:ENSMUSE00000421176"
                     /rank="3"
                     /note="ENSMUSE00000421176"
     exon            16806..16955
                     /db_xref="ENSEMBL:ENSMUSE00000283967"
                     /rank="4"
                     /note="ENSMUSE00000283967"
     misc_feature    13412..20623
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaaattca actttttttc ttcactggta aattttaaat ctgtcttcta
     4321 gaggaaaaaa acaactttag actctgtact aaagtttaag aatattgggt aacttgttag
     4381 ttacattgta accagcaatt ttgcttgctt ccaatgaccc aaactgcatg agaccaaggc
     4441 ttctcttctc catctcctgg tgtttccaaa gtgaggagat gcaggtggag tgtggtaccg
     4501 gctggcactt cttcaggaaa tagaataaaa catatttgct tttgcttttc cagtaatatg
     4561 tatgttttat tttctcagca atccagtttg caaatatttt gggttccaca aaagctgtgt
     4621 ctttgttgat tttacaagat atgtgccgaa ttttgggcct gtgtgagtgt gtgtgtgtgt
     4681 gtgtgtgtgc gtgcttgctc gagcgcgctt gcttgcatgc agtgcaaact gagatagtac
     4741 aacctgtaga cttaatctga accatacagg agggcagaaa agcactcaga tgcattctga
     4801 gtgcatcacg ttttggccac aagagggcat ctgcttattt accctgggag ccccacactc
     4861 ccgttccaac agaagcctct atcatgggta gggcgaggaa acttatgcta taacagtggt
     4921 tctcagcctg tgggtcaaga ctctgatgga ggtcaaaaag atcttttcac aggggttcag
     4981 cacttggggt gtagagttag gagatgagct agtcacttca agtgccaggg acagccgtag
     5041 gacgtgatca cacagtgaag acttgacttt ataaactgtc agctcttaat taagagcttt
     5101 ggaagtttga cacatctaga caactttcct atcccacact agataaaaaa acaatatgtt
     5161 ctttgggaaa tgattgagaa ttctcctctt ttaaaattaa aatcactctt tccaaattac
     5221 ttgaaggctg cattttagat gccttggtaa agattgtgcg tgctcctctt cctgaccccc
     5281 agctcctgtc tatggatttg actctgagtt tgcaagttga gcatctgacc taccttagaa
     5341 ctacatgctg ctggtgtggc agtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     5401 tgtagtggct gcctctcaat caatcccttt gagatgtagc ctcagcccct cctagatccc
     5461 cattcagccc aactggctca ggccatagtt gctttctctg taaaaggaaa agctgcccgg
     5521 gtgaaggcag cgggctgcac ttttgtcagc tctgcagctc tctcggtggt ggagcagctg
     5581 tagcttgctc catttagtgc aaagaaggat gcagctagca tgtgtctgcc acagctggct
     5641 ggctaccctt cacccagcaa aggagagctg gtcacttgca ctatcactgc tacagacacc
     5701 ttttatttta atggcatgaa aataaaatgt tcatttctct gggataaatt ctggggaggg
     5761 tgttactgac tagcagggca gtgggtgggg ggagggtgtg tgtgtgtgtg tgtgtgtgtg
     5821 tgtgtgtgtg tgtgtgtggc tgcctaagag gtttggcact gtcctgctct gccagagggc
     5881 cccacctttc tcattcccac cagtaatgga tgggaggtct cctttcttag catccttccc
     5941 agtatttgag attgccaggt ttttgttttg ttcccccccc ccccacttct ttttgtggtt
     6001 ctggggaatc aaaccgaagg cattgggcat gctaggaaaa tgccactgaa ccgcactagc
     6061 agcccagaac acagtggcgg ctaagactgg acatcgtctc ctgttcgtat tcaccatgct
     6121 catacatcat cattttgtga aatacctttt gtctgttccc caattaaatt gctggtgcct
     6181 ctgctgttgt gcactgaatg cggccactca gaatcacgtg tcagctctct gatgtacagg
     6241 tatttttccc taggaccaca ggctacccct tgaaacagtc atcctgccta tgcacagtca
     6301 gcggagccat gtgaacagtt gttctgctac ccttaagatc ctccattcca tccctggcac
     6361 ccaggctggc ttgctcacag gagctggggt cattctctgg cttgtcgttg tactgctaga
     6421 agttaaggcg cataacgata ccacgatatc aacaagtttg tacaaaaaag caggctggcg
     6481 ccggaaccga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcgaac
     6541 cctttcccac accaccctcc acacttgccc caaacactgc caactatgta ggaggaaggg
     6601 gttgggacta acagaagaac ccgttgtggg gaagctgttg ggagggtcac tttatgttct
     6661 tgcccaaggt cagttgggtg gcctgcttct gatgaggtgg tcccaaggtc tggggtagaa
     6721 ggtgagaggg acaggccacc aaggtcagcc ccccccccct atcccatagg agccaggtcc
     6781 ctctcctgga caggaagact gaaggggaga tgccagagac tcagtgaagc ctggggtacc
     6841 ctattggagt ccttcaagga aacaaacttg gcctcaccag gcctcagcct tggctcctcc
     6901 tgggaactct actgcccttg ggatcccctt gtagttgtgg gttacatagg aagggggacg
     6961 ggattcccct tgactggcta gcctactctt ttcttcagtc ttctccatct cctctcacct
     7021 gtctctcgac cctttcccta ggatagactt ggaaaaagat aaggggagaa aacaaatgca
     7081 aacgaggcca gaaagatttt ggctgggcat tccttccgct agcttttatt gggatcccct
     7141 agtttgtgat aggcctttta gctacatctg ccaatccatc tcattttcac acacacacac
     7201 accactttcc ttctggtcag tgggcacatg tccagcctca agtttatatc accaccccca
     7261 atgcccaaca cttgtatggc cttgggcggg tcatcccccc ccccaccccc agtatctgca
     7321 acctcaagct agcttgggtg cgttggttgt ggataagtag ctagactcca gcaaccagta
     7381 acctctgccc tttctcctcc atgacaacca ggctcccagg tcccgaaaac caaagaagaa
     7441 gaacgcagat ctctagagtc accgagttgc tttaccggat gaagagggcc gaaacatact
     7501 gtccaaggcc cttgctggca atccacccaa ctgaagccag acacaaacag aaaattgtgg
     7561 caccggtgaa acagactttg aattttgacc ttctcaagtt ggcgggagac gtcgagtcca
     7621 accccggacc ggtgcatgtg gaccccaaga aaaagcggaa ggtggaccct aaaaagaaac
     7681 gcaaagtgga tcctaagaaa aaaagaaagg tgcacgtgtc catggtgtcc aagggcgagg
     7741 aactgttcac cggcgtggtg cccatcctgg tggaactgga tggcgacgtg aacggccaca
     7801 agttcagcgt gtccggcgag ggcgaaggcg acgccacata cggaaagctg accctgaagt
     7861 tcatctgcac caccggcaag ctgcccgtgc cttggcctac cctcgtgacc acactgacct
     7921 acggcgtgca gtgcttcagc agataccccg accatatgaa gcagcacgac ttcttcaaga
     7981 gcgccatgcc cgagggctac gtgcaggaaa gaaccatctt ctttaaggac gacggcaact
     8041 acaagaccag ggccgaagtg aagttcgagg gcgacaccct cgtgaacaga atcgagctga
     8101 agggcatcga cttcaaagag gacggcaaca tcctgggcca caagctggag tacaactaca
     8161 acagccacaa cgtgtacatc atggccgaca agcagaaaaa cggcatcaaa gtgaacttca
     8221 agatccggca caacatcgag gacggctccg tgcagctggc cgaccactac cagcagaaca
     8281 cccctatcgg cgacggccct gtgctgctgc ctgacaacca ctacctgagc acccagagcg
     8341 ccctgagcaa ggaccccaac gagaagaggg accacatggt gctgctggaa ttcgtgaccg
     8401 ccgctggcat caccctgggc atggacgagc tgtacaagcc tgcaggcgag ggcagaggaa
     8461 gtcttctaac atgcggtgac gtggaggaga atcccgggcc gtccaattta ctgaccgtac
     8521 accaaaattt gcctgcatta ccggtcgatg caacgagtga tgaggttcgc aagaacctga
     8581 tggacatgtt cagggatcgc caggcgtttt ctgagcatac ctggaaaatg cttctgtccg
     8641 tttgccggtc gtgggcggca tggtgcaagt tgaataaccg gaaatggttt cccgcagaac
     8701 ctgaagatgt tcgcgattat cttctatatc ttcaggcgcg cggtctggca gtaaaaacta
     8761 tccagcaaca tttgggccag ctaaacatgc ttcatcgtcg gtctgggctg ccacgaccaa
     8821 gtgacagcaa tgctgtttca ctggttatgc ggcggatccg aaaagaaaac gttgatgccg
     8881 gtgaacgtgc aaaacaggct ctagcgttcg aacgcactga tttcgaccag gttcgttcac
     8941 tcatggaaaa tagcgaccgc tgccaggata taaggaatct ggcatttctg gggattgctt
     9001 ataacaccct gttacgtata gccgaaattg ccaggatcag ggttaaagat atctcacgta
     9061 ctgacggtgg gagaatgtta atccatattg gcagaacgaa aacgctggtt agcaccgcag
     9121 gtgtagagaa ggcacttagc ctgggggtaa ctaaactggt cgagcgatgg atttccgtct
     9181 ctggtgtagc tgatgatccg aataactacc tgttttgccg ggtcagaaaa aatggtgttg
     9241 ccgcgccatc tgccaccagc cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa
     9301 ctcatcgatt gatttacggc gctaaggatg actctggtca gagatacctg gcctggtctg
     9361 gacacagtgc ccgtgtcgga gccgcgcgag atatggcccg cgctggagtt tcaataccgg
     9421 agatcatgca agctggtggc tggaccaatg taaatattgt catgaactat atccgtaacc
     9481 tggatagtga aacaggggca atggtgcgcc tgctggaaga tggcgatctc gagccatctg
     9541 ctggagacat gagagctgcc aacctttggc caagcccgct catgatcaaa cgctctaaga
     9601 agaacagcct ggccttgtcc ctgacggccg accagatggt cagtgccttg ttggatgctg
     9661 agccccccat actctattcc gagtatgatc ctaccagacc cttcagtgaa gcttcgatga
     9721 tgggcttact gaccaacctg gcagacaggg agctggttca catgatcaac tgggcgaaga
     9781 gggtgccagg ctttgtggat ttgaccctcc atgatcaggt ccaccttcta gaatgtgcct
     9841 ggctagagat cctgatgatt ggtctcgtct ggcgctccat ggagcaccca gtgaagctac
     9901 tgtttgctcc taacttgctc ttggacagga accagggaaa atgtgtagag ggcatggtgg
     9961 agatcttcga catgctgctg gctacatcat ctcggttccg catgatgaat ctgcagggag
    10021 aggagtttgt gtgcctcaaa tctattattt tgcttaattc tggagtgtac acatttctgt
    10081 ccagcaccct gaagtctctg gaagagaagg accatatcca ccgagtcctg gacaagatca
    10141 cagacacttt gatccacctg atggccaagg caggcctgac cctgcagcag cagcaccagc
    10201 ggctggccca gctcctcctc atcctctccc acatcaggca catgagtaac aaaggcatgg
    10261 agcatctgta cagcatgaag tgcaagaacg tggtgcccct ctatgacctg ctgctggagg
    10321 cggcggacgc ccaccgccta catgcgccca ctagccgtgg aggggcatcc gtggaggaga
    10381 cggaccaaag ccacttggcc actgcgggct ctacttcatc gcattccttg caaaagtatt
    10441 acatcacggg ggaggcagag ggtttccctg ccacagcttg atgaagatct gagctcttaa
    10501 ttaatgatca taatcagcca tatcacatct gtagaggttt tacttgcttt aaaaaacctc
    10561 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
    10621 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    10681 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    10741 tggatccgtt taaactaact ttaaataatt ggcattattt aaagttacat atggcggccg
    10801 ccctaaggct cgacctcgaa attctaccgg gtaggggagg cgcttttccc aaggcagtct
    10861 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
    10921 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
    10981 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
    11041 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
    11101 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
    11161 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
    11221 aggggcgggg cgggcgcccg aaggtcttcc ggaggcccgg cattctgcac gcttcaaaag
    11281 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcatccat
    11341 ctagatctcg agcagctgaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
    11401 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
    11461 gccacgcgcc acaccgtcga tccagatcgc cacatcgagc gggtcaccga gctgcaagaa
    11521 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
    11581 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
    11641 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
    11701 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
    11761 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
    11821 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
    11881 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
    11941 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
    12001 aggagcgcac gaccccatgc atcgatgata tcagatcccc gggatgcaga aattgatgat
    12061 ctattaaaca ataaagatgt ccactaaaat ggaagttttt cctgtcatac tttgttaaga
    12121 agggtgagaa cagagtacct acattttgaa tggaaggatt ggagctacgg gggtgggggt
    12181 ggggtgggat tagataaatg cntgctcttt actgaaggct ctttactatt gctttatgat
    12241 aatgtttcat agttggatat cataatttaa acaagcaaaa ccaaattaag ggccagctca
    12301 ttcctcccac tcatgatcta tagatctata gatctctcgt gggatcattg tttttctctt
    12361 gattcccact ttgtggttct aagtactgtg gtttccaaat gtgtcagttt catagcctga
    12421 agaacgagat cagcagcctc tgttccacat acacttcatt ctcagtattg ttttgccaag
    12481 ttctaattcc atcagaagct ggtcgagtaa ctttaaataa ttggcattat ttaaagttag
    12541 gcgcgccata acttcgtata gcatacatta tacgaagtta tgtcgagata tttaaatatt
    12601 agacccagct ttcttgtaca aagtggttga tatctctata gtcgcagtag gcggacatcc
    12661 ctaagagaat ccgtggaaag gaagaaaggt ttggcatatg ctttcccctg gtcagctgcc
    12721 catcacttag agcccatggt ggctgtgact cagaactcag atggctggat ttgcctgagg
    12781 acagtgggag gcagacaaca aacacccctt cctttgtttt taatcctatt tgttggttcc
    12841 acgagcactg ccgtgtcgtc tattttgtat gtagttttag cactagaaca tgttgaatcc
    12901 ttgaaattga gcagaatggt catcaggttt gaagtgagcc acggactttg aatgctgcta
    12961 gtccctcaga aaccacagtg ctaacgtgac catctctgtt ttgctcctca aagattgaag
    13021 ctgccgtgga ggctgccaga gaggccttcc ctgcctggtc atcccggagt ccccaggaac
    13081 gttctctcgt cctgaacagg ctggctgatg tactggagca gtctctggag gagttagctc
    13141 aggcagaatc taaagaccaa ggtgacgggc atctctggca gggtctgtct gccctccaag
    13201 atctttcacc aacaggggtt gggtttgagc acaggtgtgt cttaccagat gcttatgaaa
    13261 gctggctccc tgaaccccca ttccagtttg tcaaataatc tctgcctttt actgaaatgg
    13321 atcgcacttg agagatggcg caacgcaatt aatgataact tcgtatagca tacattatac
    13381 gaagttatgg tctgagctcg ccatcagttc aagaaggcca agtcttgtgg ctgatatgct
    13441 ctgccccact gcaataagtc aggggctgat cccaaagcga catggtggga gaatacactc
    13501 tgcctatttg gtggaaggag ctacaagagt ctacagcaaa gtatggactg gggagacagg
    13561 aggacctagg ggtggccatg caatccactg ctctagtcag aggttcccga gtacagacag
    13621 tgtgaagagc agcttggctt ctgttgtgtt tcagcatttc agcccacatt ttctctccct
    13681 cccaaaggac agagggatag cgtggatacc cattaatttc ataatgtctt taggaaaagt
    13741 catgcagtat tgaaatatgc ctgccattag ccaacctaaa gttaggaatc cggtgacagg
    13801 cccttagagc agatttcttc aaagtccgtg tggtgatgct tgcctgacat cacattgctc
    13861 aggttgctga ggcaggagga ttgtaaatcc tggcagccag gcttacagtg atagcctgac
    13921 tccccttccc actctcccca cacctgaccc acacaaagga aagaagaaaa aaggaaagga
    13981 aaggaaagga aaggaaagga aaggtcaatt tttctttttt tcgtgttctt gtgtgggtgg
    14041 ttgtatttca tcactgaggg tcacctggga gaagctcaag aaaggaccct cctactccat
    14101 gcggaaccca gcatcctgtg ctgtgttctc aaccgggcct tttgatggct tctgtgtgtt
    14161 tggaggctgt ttaaggacac acacagtcat tttccaaccc cgtcgtttca aagctgctaa
    14221 cgcccgtgtg tgtggaacgg tggcatctgg tacattcatg atgaaagctt gaggtgttca
    14281 cagagacgat ctagctctat tttaaaccca cctccacctc aacctcacgc ccaaatattt
    14341 catcactcga tccatgcaag tgcggtgaag aagtgcctcg ggcgggctgg tattgagcgt
    14401 agggtcagac gactccacgt gaaacacaca ggcagcattt tatgttgttc attttccaca
    14461 gggaaaaccc tcacgctggc aaggaccatg gacattcctc ggtctgttct gaacttccgg
    14521 ttcttcgctt cctccaacct gcaccacgta tcggagtgca cacagatgag ccacctgggc
    14581 tgtatgcatt acaccgttcg caccccggtg ggaattggta ggtggcatcc aaagagaacc
    14641 tgtatcagaa agttcggaaa attctaggca ctgactacta ggggccagac taaggaaaca
    14701 caaggggtcg gtttttctac ttttgctgaa aattcgttgg ctttaaaaat gaatctagtt
    14761 cattctcaat gtgtcaaagt ctcccttttg cagaaacttc atcctgggag atcatagcag
    14821 gagggcatgt cgggagtgct ttcttgcttg taaacagaat aagaaaacat caaatccaga
    14881 cagtgccact gtttaggcgt cagggttgca ttctctgcac acttcagaac tctgaaaact
    14941 gggacgttcg ttctctttca tatttatatg attcattaag ataagaaatt tgagtaaatt
    15001 agtaagctag gggaggcagg aagatcagaa gtaccaggtt atctatcctt ggctgcatag
    15061 tgagtctgga gtcagcctgg acgacacaag accctgtctt aaaaaaaaaa aaaaaaaaga
    15121 acccaataaa taagtatgtg gcccttcaag taaggtcagt tgtctagtaa ataagcgtta
    15181 accaagttat tattttattt gcctcatcct ttaagtgtct gaaatgacta agtataatca
    15241 gtagatgtcg ctggtgtcct ttaagcgccc gaaatggcta aatctaatca gtagatattg
    15301 ctgttgtctt ttaagtgtcc gaaataacta taatcagcag atgtcgctat ttcccctggg
    15361 aagaaacacc acctttcttc tcaaatattc agattacaaa aagaaagcaa atgaaacatt
    15421 tcactctctt gattctctga acctgctaga aattatttcc caaggcatca tgggaagttt
    15481 taatttacta agaagcatct gaacatgttt tgttgtgaat atattttgtt actctgaaag
    15541 accagatttt tctttgggaa atctagtata ataaaatata cttatttcca cagggcaaaa
    15601 atataaaata gaataaatct atttttcaaa tcataaggga gacctcatat ttgtgtaggc
    15661 taaggataga aactggacac tttgagaata tttcatagag ggggctgaga gataactcag
    15721 tgattaagag tgctggctgt tcttctagag gtgctgaatt caatacccag taaccacatg
    15781 gcagctcaca accatctgta atgggatccg atgccccttc tggtacacaa gtgtacatgc
    15841 agatacagca ctcatataca ttaaataaat aaataaatct ttttaaaaat acttcagtga
    15901 gatacattca gccatttcca acttgtggtt caagatagct gtgaatgggc ataatataca
    15961 aaattgtaag ttatttataa catcatgaga tcctttgggt catttggggg agggggctca
    16021 agacagggtt tctttgtata gctccggctg tcctgtagac caggctagcc tcgaactcag
    16081 aaatccactt gcctctgcct cccaagtgct gagattaaag gcgtgtgcca ccactgcctg
    16141 gcccctttgg gtcattttaa agtaaactga ttgcctggtt cttgagtata aacttggtag
    16201 atcataattt tgtgtcacaa agtccaaaga ttgctatatt ggattttatt ttcgacctcc
    16261 tcaattgcca gggaggattt tctagtctgt atgtcagagt ccatcaacgt aaccattggc
    16321 cagtccctag tcgaatagtc acaattattt tgtgcctgtt gaatgcataa ttcagcaatg
    16381 tctgagcttt aatgtcttag tgtcaggagg ctagagacag aggtggggga gcccagcaga
    16441 aaacagcagc taaacagctt aattctgccc tgtgagaagg ctttctttaa aaaggaaaga
    16501 tcaggctaca ggtttcaata tgcattctat tccgaacaca ttggcaagct actggcagcc
    16561 ttcagccctg cagttcatat tgtcttggct cctaattata actgtagctt atgaagagga
    16621 tagaaaatgt atcgactttg ctgttgtatg gggtgaattg cttgcaaggt gtttgagaat
    16681 aacttgccag gttggcttaa agtgaagttt gggttttctg agcaggacag aattgtgtga
    16741 ggaatgccac ccagctgtct gtctctgagg cagatgagaa ctgtgctttt cttcttaccc
    16801 tgtagctggt ttgatcagcc cttggaattt accactctac ctgctgacct ggaagatagc
    16861 tccggccatc gctgctggga ataccgtgat agccaagccc agtgagatga cttctgtgac
    16921 tgcctggatg ttttgtaaac tcttggataa agcaggtaac aggatgtggg gacagggaga
    16981 agactcagag cgagggcaca cttgttagaa gcaatcttca cccaaagcta aattgactcc
    17041 agtgctgtct tgccattttg taagcctgat acttcctagg tatgccttca tccacagcag
    17101 ggttctcttc tattaacccc ataggggtgt caccatggaa cagcatcacc ccataggctg
    17161 agagcattgt agtaaatatt gcaggcttag aggtcacatc aaaggcaccg actagttcaa
    17221 gatagtatca tcaagtcact tgaaatttaa gtttgagcta ccagtcagcc ttggaagttg
    17281 ggtattccta gagttagtgt agtggaacta tgcttaatag attcagccat tttcaagagg
    17341 tttaaatatc catccccagt ttcttcctgt tttggaacca cacagaagtg gatgggacag
    17401 aacttcttat cagtacccac catttgctcc ccatccgctc tcagctaacg gtctaatgtc
    17461 cacacctagg tggacttcct ggtttcaggt gggatataac tcttcattga gaagcctgat
    17521 gttgccctgg tatttaaacc ccagcaagtg atgcttctgt cagcatcttc agcttatgaa
    17581 cccatacagc taggatggaa aagatgcctt ggtagtgggg aaggacttat cagccttctg
    17641 tctgtgtcag cgtgtggtgt gcctactgcc ttttgacaca ggggaggcac tgctgggtgc
    17701 ttctgcctcc acagaatgga atgtgcagca aggataactc tttctctctc tttctccctc
    17761 cctctctctc tctctctctc tctctctctc tctctctctc tctctctctc tctctgtgtg
    17821 tgtgtgtgtg tgtgtgtgtg tgtctccctc cctcatcata aggattttcc attttctgtg
    17881 gtttgcttac cattgctttc atttcctagt catcactaca aggcaacctc ctcttctccc
    17941 tgggctggca gcacccagtt tttcccctaa tgaacaccac actgctgctc aaactgccct
    18001 tggttataca ttgggattgg attgatgtgt gagtcataca tatgaaattc tacttttcta
    18061 caccgcagga gaaaccttga agggcgtgag tgtgaagggt aaatcaaagt ggcagtacat
    18121 cctgggtgtt tactcgtgta gccttgagcg tttctacaaa cgtgatcaat cgtcctgcag
    18181 aacaaagcca tgaagaagca ggcagcaaat atctctctgg gaattccaaa tctccttgtt
    18241 ttcttaaggc accacatctt actcctggag agattttaca gcctttccca tgagttttta
    18301 acctgctcca ttaagtttaa aattctttca ccttttcatt aagccttagt tcttaaaagg
    18361 cttcattctg gtgggttcat gtattcctgt attatctgct agccacttaa cctggtttta
    18421 ttttgtaatt tctgcgtgcc aactaaactg ttcttctctt gtaaccagaa tctcttacaa
    18481 gcttagaggg cagatacctg tgcaaacatt ttgtttctgc tgcctcttag cacgctgtca
    18541 tcagaactca aggagtcagg atgataacgc atgtgggaat cagatgctct gtggaatgag
    18601 ctcgctgctc tgtgctgaca catacacagt gttcccgtgt tttctctcag cttctgttag
    18661 aacatcacag taagcattag acacaactca gttcatttta ccattcagaa tttattaata
    18721 ttacccgacc ccccttataa ctttactctc ctgtctaccc aatgatatgg cttgccgctg
    18781 gagctcaggg cctcaaggga ttgtgggaag catggccttg cttttgccct gccagtggct
    18841 gtccttctag aatcctcttt gttctgagga ccgagttctt ctacatggta catagagctc
    18901 ttctgtctgt aaatatgaac caatattaac ctaaactaga ctcaataaac tcatacagtg
    18961 aatatagagg ctgagggctt aggtgtgaca ctgacagatt catcacaaca ttgacatccc
    19021 acctgtctgt ctcctccttt aaggagctgc ttggtggtgg tggcatgggg ctcaggggtc
    19081 ttcaccatcc cctcaaggtg accaggctta tggtgcggct cagtagaagt gagttctgga
    19141 taatactggg ttgttaatgt gaaagcacca tttatacttt aaggtcacat tcttgaagat
    19201 ttggtttgat ttgaagagag atgcctaact ggggaacggg gaggagacag gagctgccag
    19261 gctgttccaa aagcttctca cacaggattt ctgcacctgc tctagtcgcc tgaggaaaca
    19321 gagggacctc ctagatgcaa acacaacgtg accatgtcag gcttctccat ttctaaactt
    19381 gtttctgtgt ggatgctgac cgctaagcac aggtctatgt ggatgctgac tgctaagcac
    19441 aggtcggtgc actcagcccc taacatttgt taacttgctc tgaccagact gctgattgtc
    19501 atttagcttt actttgccac atttctcaat ataatcaata ataagtagta agtaataata
    19561 atcaatagta agtaccttac agggttgtga ggccagaaac ccatttgtaa tctcccctta
    19621 taagggaggg atgtgtttcc agattgtgag tagacacctg aaactgtgga tggctttggt
    19681 ctctctgtgt gccttttttt aatatatgca tatataccta tggtaaagtt taatttataa
    19741 attgggcatt atgaaaaatt aataatagaa tagaaaaggc ctaacaatgt actataataa
    19801 aacatgatgt gcaagtggcc attttcttca aatgaaaaga caaatatttc agatgataag
    19861 tgactttggg taatggaaac catagaaaac agaggatgac cacacattgc ttgtagcaat
    19921 agctatgctc acaaagcagc cactgtgtgt cagcatttac tgatggctca cggcatacca
    19981 cggggaagaa aattcagata gttcatctgc acgtggtgcc caagtgtaga ggagtaaatt
    20041 catggttggg gagctatctt agtaagttac gtgcttatct cagagcaaga ggatctgaat
    20101 tcagtctgta cagcccaggg gacagagcca ggtgtggagg tacacacaca ggagaaatcc
    20161 gggtgccggg aacaggggat cagtgtgtgc gtgcatgcgt gcatgtgtgc atgtgtgcat
    20221 gcctgttgat ctcatgctta gaggcagcca gtgtagctca ctgcagaagc tcaggctaat
    20281 gagaggctct gtctccaaaa ggatgtcttc tgaggtatgc actgagcctc tcgatacatg
    20341 tgcattcaca tgaatacaca ctcagacaca tttgcacaca cataggtatc cacatgcatt
    20401 cacatgaata catactcaga catatgtgca cacacatgtg tatccacatg cattacatga
    20461 atacacattt agacacatgc atacacatat gtacacacat gcattcacat aaatacacac
    20521 tcagacacat gtacacacac atgtgcagtc acatgaatac atagacacat ggaaataaca
    20581 catatgcaca taccctaaag aatttaatcc tgaaaacagg tat
//