LOCUS       199013#pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20577 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 199013
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000090166
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8663..8884
                     /db_xref="ENSEMBL:ENSMUSE00000635627"
                     /rank="1"
                     /note="ENSMUSE00000635627"
     exon            9066..9326
                     /db_xref="ENSEMBL:ENSMUSE00001072604"
                     /rank="2"
                     /note="ENSMUSE00001072604"
     misc_feature    4275..9829
                     /note="5 arm"
     misc_feature    9857..9881
                     /note="B1 Gateway"
     misc_feature    9894..9941
                     /note="Frt"
     misc_feature    9942..10441
                     /note="mouse En2 intron"
     primer          complement(10010..10031)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9906..9929)
                     /note="FRT3"
     primer          14093..14111
                     /note="seq primer NF"
     misc_feature    14153..14160
                     /note="RE sites"
     misc_feature    15953..15986
                     /note="loxP"
     misc_feature    complement(14161..14192)
                     /note="Rox"
     misc_feature    complement(15913..15944)
                     /note="Rox"
     misc_feature    14193..14214
                     /note="polylinker"
     CDS             14773..15369
                     /note="puromycin N-acetyl transferase"
     misc_feature    14888..14893
                     /note="Mutated RsrII site"
     intron          10442..10816
                     /note="mouse En2 intron"
     misc_feature    10814..10816
                     /note="Splice Acceptor"
     exon            10817..10856
                     /note="mouse En2 exon"
     primer          10717..10738
                     /note="seq primer FCHK2"
     misc_feature    10857..10979
                     /note="1Dleader"
     misc_feature    10980..11031
                     /note="F2A"
     misc_feature    11127..11843
                     /note="EGFP"
     misc_feature    11037..11126
                     /note="3X NLS"
     misc_feature    13909..14147
                     /note="SV40 polyadenylation site"
     misc_feature    12942..13886
                     /note="ERT2"
     promoter        14225..14736
                     /note="PGK promoter"
     misc_feature    15480..15912
                     /note="PGK pA"
     primer          13967..13990
                     /note="LRPCR RAF5"
     misc_feature    11853..11906
                     /note="T2A"
     misc_feature    11903..11903
                     /note=" A to G to kill RsRII site"
     misc_feature    11907..12932
                     /note="Cre"
     misc_feature    10832..10857
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    10832..10847
                     /note="FCHK QC prefix region"
     misc_feature    10848..10851
                     /note="FCHK QC critical region"
     misc_feature    10852..10857
                     /note="FCHK QC suffix region"
     misc_feature    16009..16029
                     /note="B2 Gateway"
     misc_feature    9830..16059
                     /note="Synthetic Cassette"
     exon            16446..16581
                     /db_xref="ENSEMBL:ENSMUSE00000586494"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000586494"
     misc_feature    16060..16738
                     /note="Critical Region"
     misc_feature    16762..16795
                     /note="Downstream LoxP"
     misc_feature    16739..16818
                     /note="synthetic loxP region"
     exon            20101..20577
                     /db_xref="ENSEMBL:ENSMUSE00000586493"
                     /rank="4"
                     /note="ENSMUSE00000586493 5' fragment"
     misc_feature    16819..20577
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggataaaat cacgaatgtg aaactatgta gcccagcttt agggagtgta
     4321 ctctgcctct agacttgaag atgtaccata tttttctctg aaagactcaa taattttata
     4381 accaggtatt tttaatacat gcacacagta tattttggtc atattcactc cactcttacc
     4441 ttaactcctc atggcatcac cgcccacctc cccatcctct ccctgcatca tgcccgtttc
     4501 tttcttaaca ccccacaggg tcaggtttat gctgctccaa tgtgcatggt gtggagccat
     4561 cccctggggc acagttgacc tgccaaggat cataccccta aaggaaactg tctcaactag
     4621 gcatgaaggc gtgtgaaccc tgcttcctgg gatgttaaat ggcttgacct tgtgcatgca
     4681 gccacagctg ctgtgagtac agtggcggtg atgtccaggg gacactgttt ctccctggtc
     4741 ttcctcaatc tctgggttct cacaatcttt ccaacccccc ttccaaaata ttgcccagcc
     4801 ctggggagct gggtgacact gatgtcccat ttatggaaag atgtgtaaca cacactatct
     4861 gcaccttaac caattgtggg tttcttcatt aactgtcatc cacagcagaa agaggtgtga
     4921 gttgcagtaa tctacgagtc aatttttatg tctttcgagg acagtttggt atgatgtcta
     4981 gttagcaata aaacagtagt aacaggttta cctctggagc ctatgcgctc cctcccagtc
     5041 atgggttttt ggccagagtt atggtgccag gcacatttcc catttgtgga gtgggcctta
     5101 aatgcaatca gaaagtggtt gatcagcccc atagcatgtc actacgacac ccatgggcac
     5161 atcccaccag tctggtcatt gctgcagctt gcagagttca gaggtaaaac tgttggtgac
     5221 ttttttctcc cagttccaca catagagccc tccagtgcta caaaagtaag ccagcgggga
     5281 gaaagcatcc cagttagaac cagcttaatt tccttctcca gttaaagcat ttcttccctc
     5341 tcccactagt ggcttctttt cattttcctg atgtgttagt taggatttct attgctgtga
     5401 taaaacacca gaatcaaaag caatttggga aggaatggct ttatgtcatc gtcccactct
     5461 cagatgacac tgcatcactg agtgaaatcg gggcaggaat taaaggcagg agctgaagca
     5521 gaggccacag aggagagcca cttagtcact cgccccgccc actaatggct tgctcatcct
     5581 gctttcatat acatctcagg accagcccag gattgcactg cccacagtat acaggaccct
     5641 cccacctcag ttaccaagac aacacaccca cagactttaa tacacaagcc catctgttga
     5701 aaaacatctt tttaattaag atgttcattt ccttgttata tctaggttac atccagttga
     5761 taaaaaccaa cccatacacc caactctaca actttccatg ttaaacatgc aaaactaaaa
     5821 atttggtgcc aagatccaca tattagtgag aacacaatga gtttgttttt ctgacaccag
     5881 gcattttata tccaattatt ttttttcatc tctcagatta tagcctctgg gatctgtggc
     5941 actgataatc ataccttaga agggaaaatg aaaacgccat ttcctgtcat cctgggccac
     6001 gagggagctg gagttgtgga gagtgttgga ccaggtgtaa ccactgtaaa gccaggtggg
     6061 taacgaggca tgatactgat gctatctagg ggtgcaggaa tgggttcagc ttctctgaca
     6121 attaaaacat taacagtcag gagaacggtg ctcccagaaa tctcggggag tcccagcaga
     6181 gccgtcatgt gatacctgag aagtcagggt ccctctggat atgtttagtt ttattatcag
     6241 aaaaaattca agagcaatcg caagcaagca tggggacaaa gattggactc aaatggaagt
     6301 gtgaagacag tttattggag cctaaaaggg aaaccgcagg tcaggcaagc tgggagggag
     6361 aggaggagtg gagaagagaa ggaacaaaag tcagtttgct tgtgctgagg ccgtctggag
     6421 gtcagcctcc tggtcgcaag tcaaggagga acgctttagg ataaagagca gggaacccta
     6481 acgcattaaa gtatcgagtg catgagcatg tataggctat gggtggccag catctgagag
     6541 aaaaagaaga aatagtcagg tcttcctagg ctggcctcag accccaagtc tcaggatggg
     6601 ttagctggta gggatagcat tatctttcag gccttctgga gaggaaaagc atattacctt
     6661 tgggtgaacc tttgggtgcc ctgtgtatta gctcttgtag gatcagacaa gggcctatct
     6721 ctgtatagag gcagattcca ttctttacaa tatagcccca gttcctttcc taattcctac
     6781 tctgattctt cccccaaggt aaaataaaga tctcagaact caagccaggt gtggtggtgc
     6841 atgcctttga tcccagctct tgggaggcag aagcaggcag atctctaagt gttagaggct
     6901 agcctggtct acagagtgag taccaggaca gccagggcta aaatccaaaa acaaaacaaa
     6961 acaaaacaaa acaaaaaaat ttaaagctca tcacatattg acaaaaccat caaacttcat
     7021 ccagacatca gatatcgtac aagatatagg cagcctggga tccagaatcc cagactgaag
     7081 gcggagttca gtggtgtgac acttgcctac catgcacaag actgtgttcc tcacaccagt
     7141 ctattggaaa ttaaactaag aattgcaata tatacaatgt atcagaatat tggtaaacat
     7201 agaatttgtg tggttacaga gcccttttac acacacacta tgtgtcctaa tctttttaag
     7261 tgtgcagagt tgcatcgtgt gtaagaattt cttctagaat ggatatttac ctcccccatg
     7321 ctccagacct tcaggtttca aacacttgat aatgtcatta tgattatcat tgccatagat
     7381 atttttgttt tccttcatgt gtactgtgag catgctatgt cagagagatg ggtgaacata
     7441 tttgtttctt tccacaatgt cagaatatac agaataacaa tatgttcaca tgatatagca
     7501 gttttcttgg cagcaatttg ctagacattg ttgcctacct tgctatcaag cactggctgt
     7561 tgattcccgt cctcgttact aatctgaact ctctgatgac atgttacaca tcacacagta
     7621 aatgcatttg tttgtgtgtg gaagagtacg gactacagaa tgagtagata tgtaggctgc
     7681 agcagcaaga aatttctagg gacctaagag gcaaagctgg aaaactgaag gaaatgcaaa
     7741 taaatagagg ctttgggaag ggtgaggtct accagatggt gcacttcctg tgttctcttg
     7801 ggagcagagt catccctctc cattctcaaa aaggagtcaa aagctacttg taaagtcacg
     7861 tctttggggg caatcaatct gaaagccccg ttttatagag ggcctcaagg caggacacag
     7921 acagatctca accatgtata aagaaataga tactctgaaa aagatcaatt accttgttta
     7981 cctaatttag ctaaacaggg ttaaaggcac aagctgtctt ttctcaacat tacaatacct
     8041 cataatgagt actgatttta atttagctca tgtataattc attcttccaa taaatattga
     8101 attctactaa ggtctaattc tacgtttctc ctggaacatt gggccaaata gaaaagtctt
     8161 ttatatatca tcaatattaa ggtcactctt acatttaact atctcttacc tttgaacctc
     8221 aatgtcatga gatgtaaata cccaagttct tttttatctt gcagatctat tatattaaaa
     8281 ttgattgttt taatctcatg gtgaaatgat agttaacttc aagtaaaaga ctccatgcaa
     8341 ctcttcttat gacaaattga tgacacctgc caaaaacagt tcccagtagt attaactgta
     8401 cttcaggcct gtcatgactt tccccataac tttctttatt ctttttcttc aggagataaa
     8461 gtcttaatgt tccctcttcc ggaatgtcga gaatgcttct actgcctcta ccctaagggc
     8521 aacttctgtg agaaagaaaa gttagtttca atgtctttga cttctcacct cattcccttt
     8581 cctcacacag ggtttggcac aaccctgata atgtctgggg ttttttttgt tttgttttgt
     8641 tcttttcctg ttgttgttgc tcagtattct ctctccaact ggattaatgt tggatggaac
     8701 cagcagattt acctgcagag ggaagaaaat ctataatata atggggacca gcacgttcac
     8761 ggaatatact gtggtgcatg agattgcagt ggcgaagatc gaggctgctg cccctatgga
     8821 tacagtctgc atcatgagct gcgcggtgcc gacaggcttt ggagctgtgt ttaacacagc
     8881 tcaggtgagt catcgttctt cctgtgttct ttccagtgac tgcctacata gtcaaggagg
     8941 agccacatag ctggaaggct ccttcatcat ggacaaaacc gaagggtttt ccccctctct
     9001 ttattcccta caaatacaaa atcactattg aaagtctaac aaaggtttat cctttaaaaa
     9061 tacaggtcac ccctggctct tcctgtgtgg tgtttggact cggaggaatt ggctctgcca
     9121 tcgtcatggc ctgcaaagcc tctggagcat gtagaatcat cggggttgac atcaatgagg
     9181 agaaatttcc tcgggcaaga gccttggggg tcaccgactg tctcaaccct aacaagctga
     9241 agaagcctgt gcaggaggtg gtgaaggaaa tgacaggggt tggtgttgac tttgcctttg
     9301 aggccatcgg actcattgaa accatggtgt gtgtcttggg tacaatgggg gcataacaca
     9361 ctaacacatt agcttcagaa agaaggaatt ttcctgtaat ttgttttatt tgggaggagt
     9421 gttgcaaatt gagcccagag cctctagcat accaaacata attctatacc tctgtagtcc
     9481 taatgtaggg ctataaattt ttccctagca tttttgagca cactgatatg ccttgcattg
     9541 ttctgagcta ctagcctatt ctatcctatt tttagatcac ctttctagat aattaataac
     9601 actatcttca ttttaaagat taaagatatt aatcaaagtg aatttaaaca gatcactcaa
     9661 ggtatcatag caaacatact ccatgctatt tgatcataaa atgtctccct gattccccct
     9721 gaaagtacac attgtcactg ttatcaagtt ctaggacaca aactgtgtac cctgtcacgc
     9781 ctggccacct attctcagta aaccaattca aaagggcaaa ttaaaagtaa aggcgcataa
     9841 cgataccacg atatcaacaa gtttgtacaa aaaagcaggc tggcgccgga accgaagttc
     9901 ctattccgaa gttcctattc tctagaaagt ataggaactt cgaacccttt cccacaccac
     9961 cctccacact tgccccaaac actgccaact atgtaggagg aaggggttgg gactaacaga
    10021 agaacccgtt gtggggaagc tgttgggagg gtcactttat gttcttgccc aaggtcagtt
    10081 gggtggcctg cttctgatga ggtggtccca aggtctgggg tagaaggtga gagggacagg
    10141 ccaccaaggt cagccccccc cccctatccc ataggagcca ggtccctctc ctggacagga
    10201 agactgaagg ggagatgcca gagactcagt gaagcctggg gtaccctatt ggagtccttc
    10261 aaggaaacaa acttggcctc accaggcctc agccttggct cctcctggga actctactgc
    10321 ccttgggatc cccttgtagt tgtgggttac ataggaaggg ggacgggatt ccccttgact
    10381 ggctagccta ctcttttctt cagtcttctc catctcctct cacctgtctc tcgacccttt
    10441 ccctaggata gacttggaaa aagataaggg gagaaaacaa atgcaaacga ggccagaaag
    10501 attttggctg ggcattcctt ccgctagctt ttattgggat cccctagttt gtgataggcc
    10561 ttttagctac atctgccaat ccatctcatt ttcacacaca cacacaccac tttccttctg
    10621 gtcagtgggc acatgtccag cctcaagttt atatcaccac ccccaatgcc caacacttgt
    10681 atggccttgg gcgggtcatc ccccccccca cccccagtat ctgcaacctc aagctagctt
    10741 gggtgcgttg gttgtggata agtagctaga ctccagcaac cagtaacctc tgccctttct
    10801 cctccatgac aaccaggctc ccaggtcccg aaaaccaaag aagaagaacg cagatctcta
    10861 gagtcaccga gttgctttac cggatgaaga gggccgaaac atactgtcca aggcccttgc
    10921 tggcaatcca cccaactgaa gccagacaca aacagaaaat tgtggcaccg gtgaaacaga
    10981 ctttgaattt tgaccttctc aagttggcgg gagacgtcga gtccaacccc ggaccggtgc
    11041 atgtggaccc caagaaaaag cggaaggtgg accctaaaaa gaaacgcaaa gtggatccta
    11101 agaaaaaaag aaaggtgcac gtgtccatgg tgtccaaggg cgaggaactg ttcaccggcg
    11161 tggtgcccat cctggtggaa ctggatggcg acgtgaacgg ccacaagttc agcgtgtccg
    11221 gcgagggcga aggcgacgcc acatacggaa agctgaccct gaagttcatc tgcaccaccg
    11281 gcaagctgcc cgtgccttgg cctaccctcg tgaccacact gacctacggc gtgcagtgct
    11341 tcagcagata ccccgaccat atgaagcagc acgacttctt caagagcgcc atgcccgagg
    11401 gctacgtgca ggaaagaacc atcttcttta aggacgacgg caactacaag accagggccg
    11461 aagtgaagtt cgagggcgac accctcgtga acagaatcga gctgaagggc atcgacttca
    11521 aagaggacgg caacatcctg ggccacaagc tggagtacaa ctacaacagc cacaacgtgt
    11581 acatcatggc cgacaagcag aaaaacggca tcaaagtgaa cttcaagatc cggcacaaca
    11641 tcgaggacgg ctccgtgcag ctggccgacc actaccagca gaacacccct atcggcgacg
    11701 gccctgtgct gctgcctgac aaccactacc tgagcaccca gagcgccctg agcaaggacc
    11761 ccaacgagaa gagggaccac atggtgctgc tggaattcgt gaccgccgct ggcatcaccc
    11821 tgggcatgga cgagctgtac aagcctgcag gcgagggcag aggaagtctt ctaacatgcg
    11881 gtgacgtgga ggagaatccc gggccgtcca atttactgac cgtacaccaa aatttgcctg
    11941 cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac atgttcaggg
    12001 atcgccaggc gttttctgag catacctgga aaatgcttct gtccgtttgc cggtcgtggg
    12061 cggcatggtg caagttgaat aaccggaaat ggtttcccgc agaacctgaa gatgttcgcg
    12121 attatcttct atatcttcag gcgcgcggtc tggcagtaaa aactatccag caacatttgg
    12181 gccagctaaa catgcttcat cgtcggtctg ggctgccacg accaagtgac agcaatgctg
    12241 tttcactggt tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa cgtgcaaaac
    12301 aggctctagc gttcgaacgc actgatttcg accaggttcg ttcactcatg gaaaatagcg
    12361 accgctgcca ggatataagg aatctggcat ttctggggat tgcttataac accctgttac
    12421 gtatagccga aattgccagg atcagggtta aagatatctc acgtactgac ggtgggagaa
    12481 tgttaatcca tattggcaga acgaaaacgc tggttagcac cgcaggtgta gagaaggcac
    12541 ttagcctggg ggtaactaaa ctggtcgagc gatggatttc cgtctctggt gtagctgatg
    12601 atccgaataa ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg ccatctgcca
    12661 ccagccagct atcaactcgc gccctggaag ggatttttga agcaactcat cgattgattt
    12721 acggcgctaa ggatgactct ggtcagagat acctggcctg gtctggacac agtgcccgtg
    12781 tcggagccgc gcgagatatg gcccgcgctg gagtttcaat accggagatc atgcaagctg
    12841 gtggctggac caatgtaaat attgtcatga actatatccg taacctggat agtgaaacag
    12901 gggcaatggt gcgcctgctg gaagatggcg atctcgagcc atctgctgga gacatgagag
    12961 ctgccaacct ttggccaagc ccgctcatga tcaaacgctc taagaagaac agcctggcct
    13021 tgtccctgac ggccgaccag atggtcagtg ccttgttgga tgctgagccc cccatactct
    13081 attccgagta tgatcctacc agacccttca gtgaagcttc gatgatgggc ttactgacca
    13141 acctggcaga cagggagctg gttcacatga tcaactgggc gaagagggtg ccaggctttg
    13201 tggatttgac cctccatgat caggtccacc ttctagaatg tgcctggcta gagatcctga
    13261 tgattggtct cgtctggcgc tccatggagc acccagtgaa gctactgttt gctcctaact
    13321 tgctcttgga caggaaccag ggaaaatgtg tagagggcat ggtggagatc ttcgacatgc
    13381 tgctggctac atcatctcgg ttccgcatga tgaatctgca gggagaggag tttgtgtgcc
    13441 tcaaatctat tattttgctt aattctggag tgtacacatt tctgtccagc accctgaagt
    13501 ctctggaaga gaaggaccat atccaccgag tcctggacaa gatcacagac actttgatcc
    13561 acctgatggc caaggcaggc ctgaccctgc agcagcagca ccagcggctg gcccagctcc
    13621 tcctcatcct ctcccacatc aggcacatga gtaacaaagg catggagcat ctgtacagca
    13681 tgaagtgcaa gaacgtggtg cccctctatg acctgctgct ggaggcggcg gacgcccacc
    13741 gcctacatgc gcccactagc cgtggagggg catccgtgga ggagacggac caaagccact
    13801 tggccactgc gggctctact tcatcgcatt ccttgcaaaa gtattacatc acgggggagg
    13861 cagagggttt ccctgccaca gcttgatgaa gatctgagct cttaattaat gatcataatc
    13921 agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
    13981 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
    14041 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    14101 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgtttaaac
    14161 taactttaaa taattggcat tatttaaagt tacatatggc ggccgcccta aggctcgacc
    14221 tcgaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
    14281 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
    14341 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
    14401 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
    14461 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
    14521 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
    14581 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
    14641 gcccgaaggt cttccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
    14701 ctgttctcct cttcctcatc tccgggcctt tcgacctgca tccatctaga tctcgagcag
    14761 ctgaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
    14821 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
    14881 gtcgatccag atcgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
    14941 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
    15001 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
    15061 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
    15121 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
    15181 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
    15241 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
    15301 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
    15361 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
    15421 catgcatcga tgatatcaga tccccgggat gcagaaattg atgatctatt aaacaataaa
    15481 gatgtccact aaaatggaag tttttcctgt catactttgt taagaagggt gagaacagag
    15541 tacctacatt ttgaatggaa ggattggagc tacgggggtg ggggtggggt gggattagat
    15601 aaatgcntgc tctttactga aggctcttta ctattgcttt atgataatgt ttcatagttg
    15661 gatatcataa tttaaacaag caaaaccaaa ttaagggcca gctcattcct cccactcatg
    15721 atctatagat ctatagatct ctcgtgggat cattgttttt ctcttgattc ccactttgtg
    15781 gttctaagta ctgtggtttc caaatgtgtc agtttcatag cctgaagaac gagatcagca
    15841 gcctctgttc cacatacact tcattctcag tattgttttg ccaagttcta attccatcag
    15901 aagctggtcg agtaacttta aataattggc attatttaaa gttaggcgcg ccataacttc
    15961 gtatagcata cattatacga agttatgtcg agatatttaa atattagacc cagctttctt
    16021 gtacaaagtg gttgatatct ctatagtcgc agtaggcggt tctaggtcct gaaatgagag
    16081 caaggttcaa aacagataca cacatctaag cagtcctggt cacggcgtga tctcacctac
    16141 cctgagccaa tccttgtctg ggcttcagtc tcatcctgta aggaagtcta acaagctgtt
    16201 agatctgtgt gaaatctttc tccaggagac aagtttcccc tctggctggt gcccttctct
    16261 tctcctagca tgagattcat gagaaaacac ccatttcctt gaggttagtt gtttcaatag
    16321 tctgcttgtc tgatggagag gtgcacaggc ctctttagaa catcttcact cctatcgggc
    16381 cagttacaat ttacatgtat gtgtaaatgc cactgagata ttaaccagtc tgtttctgtg
    16441 tttaggttgc ggctttgaag tcatgcaata ggagctacgg agtgtgtgtg attatgggag
    16501 tagctccgac cggttcccag ctctcttttg acccattggt acttctccct ggaagaaccc
    16561 taaaatcttc tgttttggga ggtaggtctc tcagtttcct ctctctttta gaaccctaaa
    16621 tccccctgca tgctctttgg aaggaaacaa ggaaggaaga ccggaagaag tagaaggcaa
    16681 ggcagtgtat caaaaatgat aaggtgaggt gggcctggag tctcttgcat acaagccaga
    16741 gatggcgcaa cgcaattaat gataacttcg tatagcatac attatacgaa gttatggtct
    16801 gagctcgcca tcagttcagc agtcaggtat acacagaaag accctcttct aacttccaaa
    16861 taggacaagt ctatcttccc gaaagcagtg tgcccctgta gaaacaaaca gagtaccagt
    16921 ccaacgctga aaagacatag aagatgtact cggtgacaag ccatggatgg cctatggacc
    16981 actcctccgt agagaggaat atatcaacaa gccatggatg gcctatggac cactcctccg
    17041 taaagaggaa tatatcactg atttggtccc tgcgttctca tctttatact cagaccactt
    17101 ttctatttta aaacaaaaca gatcattaca atgtgggtca caattcatcc tctcttctct
    17161 aaatacctcc agattctatt ccatcactgg tcatacccca tatgcctccc agcctaacat
    17221 gtctcgggga acacgtgcag tgataaatgt tctgaggcca tattgtctaa tgcagtgact
    17281 tctggtcgca tgtggctatt ggctactcag aagatggcta gtgggacccg gaggatgatg
    17341 agtcagttgt gtttcactct tctttattta aattcgaaga acgacagtgg atgtcacagc
    17401 acatgcctca ctgcacgtgc taggaacaca gagggaagtc gatttctgtg agttcaaggc
    17461 taccttgatc tgcatagtga ctaagtttca gggtagccag ggtgacatac tgagaccttg
    17521 cctaaaaagt aaggaaataa atgcaagcaa tcaagtatga ctagcattgc cgtattatta
    17581 gatagcacgg ctcatattat cttataccct agccttaacc agagtactca agcaagcctg
    17641 aggattgatg agtggtcatt ggtgattgat taatgatgga ttgattggtt gattgattga
    17701 tggttgttaa tttaaggtct ggaatcgaaa aacttggctt ttggctgtca gagttctcga
    17761 tgggcactac ccataaacaa gtcattcatt ctcttaatcc cagtccaggg tgagcagtag
    17821 cttttcttat agagtgagaa ctaaatgaaa aatatacaca ttaaagcgtt taccagtaaa
    17881 aataagccat cagttgaggt attggttatg gtagcccaca acccaagaaa acctgaggcc
    17941 caagactgcc aaagataacc aaagtggctt ggctttgctt tggtggcaat gtgtgtttgt
    18001 ttgttggctt gaatctctgg gaccctctcc attattcctt gacaggcttt tgtgtaagtg
    18061 caatatgaag ttggattact gcccagcttt aacagagaga atgtttccac tgaccagtct
    18121 tccactataa aaatttcatc tttcagacac tcaaaactta tctaactgtg taaaacttcc
    18181 caaactaacc catgtaaaga gaaactgagt gctgagaata gctatctatc cttgtccatt
    18241 ttctgttaag gctaagaaaa acacctgtgg gtagttaatg tataagaaaa agacgtttgt
    18301 tttgcttttg ttaagatttt tttttattca tttttcttct tatgtgtgag tgttttgctg
    18361 catgtctgta tatgcatcat ttgtgtgctt ggtgccctca aagattacat ggaagagggt
    18421 ctcagagcct ctaaatctga agttacagat ggtcatgagc aactatgtga gggctaggaa
    18481 ttggtcccag gtctgctaga agagacacta gtgctcttta gtactaagcc acctctccaa
    18541 ccgaagtcag gatattttta tcagagcaac agaaaaaaga atctgaaatt gacatcaaat
    18601 aggaagaggg gggatttgta tagccaagat gacctcaagt gtgtggcata ggattcagac
    18661 ccatgcctgt ggctgtcggg tacagccttc tggttcagca ccaaggtcta ctctgcagca
    18721 ggagcagtac ccactcagca agagagaagc tgggaagtaa acatcagggg aaggattgcc
    18781 tgccgggctc tcactgggcc aatggctctt cctgtcccaa gacaaggttc tctgctgccc
    18841 tcctctcatg agactgacat cgtccaggtt ctgtagtttc ttttcctgtc aggcactatg
    18901 ctaccttaga ggcttttcct tgcttttagg aggaccctag aaacccaagc aacggctgca
    18961 aaagtgagag tgtttctctc caggtgtctg ggtttaccag ttttgtgcct ggctgcccta
    19021 tttccagatt tcaattttgt acctttattt tcaccagcta ccaatgtcaa aatctacagt
    19081 ttattcccaa accttcttgc tgtgctgtgt gtgtctcctg gtgctattga cgacaccaag
    19141 tgtgtaatgg cgtgagagcg tgctgtagag gtacaccaga tactcaccct tggttctcag
    19201 ttaccagtga ataaaactaa taaacttagt ccctttaaaa gaattaataa aattcccaaa
    19261 caataaaaat gttatggaaa aaaaggaatt atttcattat agatgaaaga agctataata
    19321 gacatagtct tgtttttcca gttttatgct aaccggtttc taggctagtg aaaaacctgc
    19381 ataccaactg gttgtgcact catcagttat tgtattgtat ctgtgatatg gatgcttaat
    19441 gtccctcagg tatgtaagtt aaaggtcccc aggatggtgc tactgatatg ttctgtggtc
    19501 ctttcggagg taagtcttct gggaaggcct taggtcactg gatgtacgat ttcacatgat
    19561 tctgtgctgg tgttttttcc tctgtaaaag aaaagaacag aaaaaataga ctcaatgcaa
    19621 tcatgctcga agtgcatcag cccatggctc tccattgttc tcaggacact aactacttcc
    19681 tataatgttc accccccagc tgatttgatc tgagttaatc tcagaatctt gaatgaagtg
    19741 agactggcaa cccagatcaa tgagctagcg agggtgcagg aaatggtcag tgccctgaag
    19801 acaaagaaga gctttagcta catgggttat agccatgagg gtgtcaggta ggagcgctct
    19861 cctagggccc tggtaatcat aattctgtcc atgactggat tcttctatgg aacaaactgc
    19921 tttgacactt ggataaagaa tcaatggggt aatgaatgac ttactgcacc cataaacaca
    19981 ttgataattg tataaattat taaatttatt atagattatt aattataata ttatcactgg
    20041 aatcttcagt cagatttgat atgtttcaca tcctattaat tttgtccttt ccttctacag
    20101 ggtataaaac cagagatgac atccctaaac tagtaactga ctacgttcaa aagaaaatta
    20161 atatagatcc tcttataacc catagactgc ctttcccaaa aatcaatgaa ggattcagat
    20221 tgttgcaaga gggaaaatgg taagtattca tgtgaatggg gtgagtgtgt gtgtgtgtgt
    20281 gtgtgtgtgt gtgtgtacaa gacattcctg ggtgtggtga catacatgtg acttccagtc
    20341 aaaatcttaa gaaaagccct ctatgctccc atataacttt ccattaccct catgatgact
    20401 tgtgacagta aatggcaatt aacctgcctg atgagttttt gaatatcaca tttagatctc
    20461 taacaccagc ctcaactgca atatgtcaag tgcaggcatt ggtcaggaat aagctttggc
    20521 ttaaaagaac aaataagtag gaaggtaaac ggataaaagc aaactttcca cccatga
//