LOCUS       44771#pL1L2_GT2_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20861 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT2_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 44771
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000039557
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..8773
                     /note="5 arm"
     misc_feature    8801..8825
                     /note="B1 Gateway"
     misc_feature    8838..8885
                     /note="Frt"
     misc_feature    8886..9385
                     /note="mouse En2 intron"
     primer          complement(8954..8975)
                     /note="LRPCR LAR3_EN2"
     primer          complement(8850..8873)
                     /note="FRT3"
     primer          13044..13062
                     /note="seq primer NF"
     misc_feature    13104..13111
                     /note="RE sites"
     misc_feature    14904..14937
                     /note="loxP"
     misc_feature    complement(13112..13143)
                     /note="Rox"
     misc_feature    complement(14864..14895)
                     /note="Rox"
     misc_feature    13144..13165
                     /note="polylinker"
     CDS             13724..14320
                     /note="puromycin N-acetyl transferase"
     misc_feature    13839..13844
                     /note="Mutated RsrII site"
     intron          9386..9760
                     /note="mouse En2 intron"
     misc_feature    9758..9760
                     /note="Splice Acceptor"
     exon            9761..9807
                     /note="mouse En2 exon"
     primer          9661..9682
                     /note="seq primer FCHK2"
     misc_feature    9808..9930
                     /note="1Dleader"
     misc_feature    9931..9982
                     /note="F2A"
     misc_feature    10078..10794
                     /note="EGFP"
     misc_feature    9988..10077
                     /note="3X NLS"
     misc_feature    12860..13098
                     /note="SV40 polyadenylation site"
     misc_feature    11893..12837
                     /note="ERT2"
     promoter        13176..13687
                     /note="PGK promoter"
     misc_feature    14431..14863
                     /note="PGK pA"
     primer          12918..12941
                     /note="LRPCR RAF5"
     misc_feature    10804..10857
                     /note="T2A"
     misc_feature    10854..10854
                     /note="A to G to kill RsRII site"
     misc_feature    10858..11883
                     /note="Cre"
     misc_feature    9776..9808
                     /note="FCHK lf2a_lacz_gt2"
     misc_feature    9776..9791
                     /note="FCHK QC prefix region"
     misc_feature    9792..9802
                     /note="FCHK QC critical region"
     misc_feature    9803..9808
                     /note="FCHK QC suffix region"
     misc_feature    14960..14980
                     /note="B2 Gateway"
     misc_feature    8774..15010
                     /note="Synthetic Cassette"
     exon            15363..15559
                     /db_xref="ENSEMBL:ENSMUSE00001298296"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00001298296"
     misc_feature    15011..15764
                     /note="Critical Region"
     misc_feature    15788..15821
                     /note="Downstream LoxP"
     misc_feature    15765..15844
                     /note="synthetic loxP region"
     exon            19348..19583
                     /db_xref="ENSEMBL:ENSMUSE00000308913"
                     /rank="3"
                     /note="ENSMUSE00000308913"
     misc_feature    15845..20861
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggagttaag gaaacaggat atcaatgcaa atcttatctt ctgcagactg
     4321 agtttctcta gaaacactgc aaattatgtc atcagaactg atagaaaatc catttgagta
     4381 tagcgagggt tgttgatgcc tttctctcca gtggttgagg tatcctggtt acttggcaaa
     4441 attcctaggg gagggtactt gagaaggttg tatttcattg agaagtttac ttcttcagat
     4501 aagaagatgg gagagagtcc tttcatgctt tggggaagaa gaaggaagag aaaattagat
     4561 agtccttgtt tcagagaata gtcctaaggc cttccacttt ctttttaatt caaaagcacg
     4621 ccaaaaccat actatgaaat attgtttttt taagccttaa ggagttaggg tacagaaaat
     4681 aaaaattgga ccagattgct aaatatggat tccagttcat atcctctagt taaaaataaa
     4741 tctgcctttt ggtgacttta ggaaagagac catggaggac ccaaaggcac agaagaaata
     4801 tgtaccatca aataccctgt caaacgggaa gttgctctct tcatttttag gaatagatgt
     4861 cagtgaatga tgaaggacat tgacaaggtg gggcttttga aatgacattt taatcctttg
     4921 tcatcatgct ctaagtatga agatactttt ggaataaatc ccatttttat ttaaagtgga
     4981 agtctgcaaa actaaatgaa aacaaagaag taggtcaaga ggcactagga aggcatccct
     5041 gcttttctca gtggggttgt ctagagcttc ccccatacag ctaacttgtt ccttttcaga
     5101 ccattggtgt tcttgagaag tgaaaaatta gtggttttca aaatgtggtg gggggatggg
     5161 ggagtgtaaa actcctttca gtggactcaa aatcaaaact gtcttcatag ggtcactgtg
     5221 ccatttgctt tttttttggt tctcattctg tcgtgagaac atagtgacaa acctcagggc
     5281 atgtgggaca tagccagaga ctgagtgtag gagcagctat gagcatccag atggcctctg
     5341 tgaagctgga ttttaaaggt agttccacaa acaacagggc tgctcctcta tctcccttct
     5401 cggtcatctg tttttttact gcctctttct cttcctcctc ctccttctcc tccttttccc
     5461 cctccttctt cctcttcctt ttccttcttc ctcttactgc cacttcctct ttctcctcct
     5521 tcctcctgtc ttcctccctc ctttttctcc tccttcttcc tcttccttcc tcttcctcct
     5581 ctttcctcct gtcttcctcc tttctccttc tccttcctcc tccttctttc tcctttcttc
     5641 ctcctctctc cctttccctc ctccttcatc ctccttctcc ctcctccttc ctcctttttc
     5701 tttctccttc ttcttcctcc tcctccttcc ttcttcctta ttcttgcttg ctgttttttc
     5761 ttcctttgtc attttttttt tcttgtcttc gttgtcattg tctccttcct tttctttgtg
     5821 ttcttgttct tgttccaaaa acattacaat tgtgtttttt ttttttttgt tttgtttttt
     5881 tttgttttcc gagacagggt ttctctgtgt agccctggct gtcttggaac tcactttgta
     5941 gatcagactg gcctcaaact cagaaatccg cctgcctctg cctcccgagt gctggaatta
     6001 aaggagtacg ccaccacgcc cagccaaaac attacattct tagcaaatga atagtgctta
     6061 attttttcag cttcaatatc taatgctgta atattaatta tatattatgt attaataata
     6121 atagataaaa aattcacaag gaaatgcttt tagcaccatt tgctggctga gatcgtgtga
     6181 aaataaaatc tcctcctgct caactttatt acacctagca gatggtgctt tattgtttta
     6241 aactcatttt ggaataagca ttaatataaa agacctagtc cttaaaacaa aaaagagaaa
     6301 atacggtaat tgaccattta ctaggatagt tttaagaagg ttcctaactt ttagctcttg
     6361 tctgtccata ggataaaagt taagtatgtt ttcaaaagaa aaaaagggat acaatatgtg
     6421 aactggccac acctggaagc aagtttgtat taagagaaaa aattaaaaat tagattgaca
     6481 gaacctagca aaggatggga aattatgaac acttgctgaa tcccattatt tattatccta
     6541 atacttaaaa tggaaaatgt agttcaagaa tatttaccag taatgcaaaa ggaaattaaa
     6601 aaaaaataaa aacaaaaagg gttcatgatt tcgtgatttt ttttttcaat taagcaccta
     6661 aatctaacca agtcccagta ctgagtcatt tcttcagcac tgaggtataa gattgatgct
     6721 ctgtgaatgt tttcacagca gggcttgaca gcgaagctag gtaacaaagc tgtaattcag
     6781 tgccctggaa tttttctgca catgctatct taatggaaat ttaccagaaa tttcttgcca
     6841 gaataaacac atttccttca ggaatgaaag gcttggtttt cgtctattca gccgcacctc
     6901 tttcctgtga gcagataaac caaattattt ggtattagtt attatacttc tcccagttca
     6961 cgggactcat cttagtctct aacagggacc agaagagcta gaagtatgga ataagaagat
     7021 ggcagtcaat tttatactcc agtgagagag aaaacaaaag gaaagacatg aggatcgcac
     7081 agttaacatg aaataataca acagcaaaaa gtgaatgggg acatctgtga atcggcttta
     7141 ggtattctga caggtgtcct gattaaggat gtgaaccaca cacaggtgaa tggggctgct
     7201 gattctggac gtgtaaacat tgtatgagac agggtgactt tttatgaatg attttcaacc
     7261 aagactgaag actctatact gacagtcctg actatacagt gataagtggc attaggcgcc
     7321 cagctcggga gcaacattcc tttgacatac cctggatgag tgggctagga gggaggatag
     7381 atatcgcctt tgagttaagg atggaggagg cccacagtgg acaatggaga ctggatgaaa
     7441 gagattccca gacttccctc catttcttca ttaaccctgt ccctgagatc cacttcaagg
     7501 ttactgtcag ggaaagaggt aatatttatg gggtgggtgt ctcactccat agcccagggt
     7561 gacctgacat ttgaaatggt gctgggttag ttgactgagt gttgaggttg ctagggtggg
     7621 gtgccatacc ctactccgag gtaagactgc aaagatcttt cctttcattc tcacgagcag
     7681 caatatgaaa tacttaagat ccacagcgcc agccttagca agtaaacctt gcctaccagc
     7741 gcatcgcagc tctgtctcca gttatggccc atttctttgg aagtttttct tgacatgttt
     7801 ttcattttaa caaactattt ggcattcaac tggataaatt tgtctgagtc taaggacctg
     7861 ctccctcaca cggcatttag agcctactct ttaaataata attggaagga gcagtttaga
     7921 tgcgaggata tcattcatgg attaataata aattttatct tattgtgttt ctcttttttg
     7981 tcaatatgta caggaaataa aaccagtcta gcaattattg cttcttagct atccttggga
     8041 gataaaatac actttgaaat tagaataata aaacactttc caccaactat tatcccactc
     8101 aaacaatatt ttttaaataa aaaaatcatt ttatgtaaag aaaaaaacac cagagatgaa
     8161 agtgctgttt ctttgaagtg cttacgtgcc gttttctttc tatctgtaaa taataagaaa
     8221 taaaaattac tttctattct gtattggttt ttattgtaga ttctcataat tcaatgttag
     8281 aagtaggttg tattaagtct aaggaaaagt agtttagttg gtgtcataaa gatctcattc
     8341 aagagctgct gagacagaca gcaggttctc tgccaagaca tcacttgctg ccaagtccgg
     8401 tgactatagt tcaacgccag ggacctacac aatagcatga gagaactgag tctactgaga
     8461 tgtgccttga cttccacaca tactccatga tttgtgtgcc ctctcctacc cacagtaaat
     8521 atgtgaagtg aaagtaaatc tcattcaatt tattctttac ataaataaca ggaaatgtat
     8581 gaggcaattt caagttatcc agaatttcca gtaaatgtgt atttagacaa attattcata
     8641 tgtacaagtt atgagtgcat ttataagttt ttaaagtcct tccttcagta gactgtaaag
     8701 gaaatttcat aaattaactt ctgtatattt cactttggaa aataagatag aaatagcaat
     8761 agcaccttac tcaaaggcgc ataacgatac cacgatatca acaagtttgt acaaaaaagc
     8821 aggctggcgc cggaaccgaa gttcctattc cgaagttcct attctctaga aagtatagga
     8881 acttcgaacc ctttcccaca ccaccctcca cacttgcccc aaacactgcc aactatgtag
     8941 gaggaagggg ttgggactaa cagaagaacc cgttgtgggg aagctgttgg gagggtcact
     9001 ttatgttctt gcccaaggtc agttgggtgg cctgcttctg atgaggtggt cccaaggtct
     9061 ggggtagaag gtgagaggga caggccacca aggtcagccc ccccccccta tcccatagga
     9121 gccaggtccc tctcctggac aggaagactg aaggggagat gccagagact cagtgaagcc
     9181 tggggtaccc tattggagtc cttcaaggaa acaaacttgg cctcaccagg cctcagcctt
     9241 ggctcctcct gggaactcta ctgcccttgg gatccccttg tagttgtggg ttacatagga
     9301 agggggacgg gattcccctt gactggctag cctactcttt tcttcagtct tctccatctc
     9361 ctctcacctg tctctcgacc ctttccctag gatagacttg gaaaaagata aggggagaaa
     9421 acaaatgcaa acgaggccag aaagattttg gctgggcatt ccttccgcta gcttttattg
     9481 ggatccccta gtttgtgata ggccttttag ctacatctgc caatccatct cattttcaca
     9541 cacacacaca ccactttcct tctggtcagt gggcacatgt ccagcctcaa gtttatatca
     9601 ccacccccaa tgcccaacac ttgtatggcc ttgggcgggt catccccccc cccaccccca
     9661 gtatctgcaa cctcaagcta gcttgggtgc gttggttgtg gataagtagc tagactccag
     9721 caaccagtaa cctctgccct ttctcctcca tgacaaccag gctcccaggt cccgaaaacc
     9781 aaagaagaag aacgcagatc gcagatctct agagtcaccg agttgcttta ccggatgaag
     9841 agggccgaaa catactgtcc aaggcccttg ctggcaatcc acccaactga agccagacac
     9901 aaacagaaaa ttgtggcacc ggtgaaacag actttgaatt ttgaccttct caagttggcg
     9961 ggagacgtcg agtccaaccc cggaccggtg catgtggacc ccaagaaaaa gcggaaggtg
    10021 gaccctaaaa agaaacgcaa agtggatcct aagaaaaaaa gaaaggtgca cgtgtccatg
    10081 gtgtccaagg gcgaggaact gttcaccggc gtggtgccca tcctggtgga actggatggc
    10141 gacgtgaacg gccacaagtt cagcgtgtcc ggcgagggcg aaggcgacgc cacatacgga
    10201 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccttg gcctaccctc
    10261 gtgaccacac tgacctacgg cgtgcagtgc ttcagcagat accccgacca tatgaagcag
    10321 cacgacttct tcaagagcgc catgcccgag ggctacgtgc aggaaagaac catcttcttt
    10381 aaggacgacg gcaactacaa gaccagggcc gaagtgaagt tcgagggcga caccctcgtg
    10441 aacagaatcg agctgaaggg catcgacttc aaagaggacg gcaacatcct gggccacaag
    10501 ctggagtaca actacaacag ccacaacgtg tacatcatgg ccgacaagca gaaaaacggc
    10561 atcaaagtga acttcaagat ccggcacaac atcgaggacg gctccgtgca gctggccgac
    10621 cactaccagc agaacacccc tatcggcgac ggccctgtgc tgctgcctga caaccactac
    10681 ctgagcaccc agagcgccct gagcaaggac cccaacgaga agagggacca catggtgctg
    10741 ctggaattcg tgaccgccgc tggcatcacc ctgggcatgg acgagctgta caagcctgca
    10801 ggcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cgggccgtcc
    10861 aatttactga ccgtacacca aaatttgcct gcattaccgg tcgatgcaac gagtgatgag
    10921 gttcgcaaga acctgatgga catgttcagg gatcgccagg cgttttctga gcatacctgg
    10981 aaaatgcttc tgtccgtttg ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa
    11041 tggtttcccg cagaacctga agatgttcgc gattatcttc tatatcttca ggcgcgcggt
    11101 ctggcagtaa aaactatcca gcaacatttg ggccagctaa acatgcttca tcgtcggtct
    11161 gggctgccac gaccaagtga cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa
    11221 gaaaacgttg atgccggtga acgtgcaaaa caggctctag cgttcgaacg cactgatttc
    11281 gaccaggttc gttcactcat ggaaaatagc gaccgctgcc aggatataag gaatctggca
    11341 tttctgggga ttgcttataa caccctgtta cgtatagccg aaattgccag gatcagggtt
    11401 aaagatatct cacgtactga cggtgggaga atgttaatcc atattggcag aacgaaaacg
    11461 ctggttagca ccgcaggtgt agagaaggca cttagcctgg gggtaactaa actggtcgag
    11521 cgatggattt ccgtctctgg tgtagctgat gatccgaata actacctgtt ttgccgggtc
    11581 agaaaaaatg gtgttgccgc gccatctgcc accagccagc tatcaactcg cgccctggaa
    11641 gggatttttg aagcaactca tcgattgatt tacggcgcta aggatgactc tggtcagaga
    11701 tacctggcct ggtctggaca cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct
    11761 ggagtttcaa taccggagat catgcaagct ggtggctgga ccaatgtaaa tattgtcatg
    11821 aactatatcc gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc
    11881 gatctcgagc catctgctgg agacatgaga gctgccaacc tttggccaag cccgctcatg
    11941 atcaaacgct ctaagaagaa cagcctggcc ttgtccctga cggccgacca gatggtcagt
    12001 gccttgttgg atgctgagcc ccccatactc tattccgagt atgatcctac cagacccttc
    12061 agtgaagctt cgatgatggg cttactgacc aacctggcag acagggagct ggttcacatg
    12121 atcaactggg cgaagagggt gccaggcttt gtggatttga ccctccatga tcaggtccac
    12181 cttctagaat gtgcctggct agagatcctg atgattggtc tcgtctggcg ctccatggag
    12241 cacccagtga agctactgtt tgctcctaac ttgctcttgg acaggaacca gggaaaatgt
    12301 gtagagggca tggtggagat cttcgacatg ctgctggcta catcatctcg gttccgcatg
    12361 atgaatctgc agggagagga gtttgtgtgc ctcaaatcta ttattttgct taattctgga
    12421 gtgtacacat ttctgtccag caccctgaag tctctggaag agaaggacca tatccaccga
    12481 gtcctggaca agatcacaga cactttgatc cacctgatgg ccaaggcagg cctgaccctg
    12541 cagcagcagc accagcggct ggcccagctc ctcctcatcc tctcccacat caggcacatg
    12601 agtaacaaag gcatggagca tctgtacagc atgaagtgca agaacgtggt gcccctctat
    12661 gacctgctgc tggaggcggc ggacgcccac cgcctacatg cgcccactag ccgtggaggg
    12721 gcatccgtgg aggagacgga ccaaagccac ttggccactg cgggctctac ttcatcgcat
    12781 tccttgcaaa agtattacat cacgggggag gcagagggtt tccctgccac agcttgatga
    12841 agatctgagc tcttaattaa tgatcataat cagccatatc acatctgtag aggttttact
    12901 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    12961 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    13021 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    13081 tgtatcttat catgtctgga tccgtttaaa ctaactttaa ataattggca ttatttaaag
    13141 ttacatatgg cggccgccct aaggctcgac ctcgaaattc taccgggtag gggaggcgct
    13201 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
    13261 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
    13321 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
    13381 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
    13441 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
    13501 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
    13561 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcttccggag gcccggcatt
    13621 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
    13681 ttcgacctgc atccatctag atctcgagca gctgaagctt accatgaccg agtacaagcc
    13741 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
    13801 gttcgccgac taccccgcca cgcgccacac cgtcgatcca gatcgccaca tcgagcgggt
    13861 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
    13921 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
    13981 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
    14041 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
    14101 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
    14161 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
    14221 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
    14281 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
    14341 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg atgatatcag atccccggga
    14401 tgcagaaatt gatgatctat taaacaataa agatgtccac taaaatggaa gtttttcctg
    14461 tcatactttg ttaagaaggg tgagaacaga gtacctacat tttgaatgga aggattggag
    14521 ctacgggggt gggggtgggg tgggattaga taaatgcntg ctctttactg aaggctcttt
    14581 actattgctt tatgataatg tttcatagtt ggatatcata atttaaacaa gcaaaaccaa
    14641 attaagggcc agctcattcc tcccactcat gatctataga tctatagatc tctcgtggga
    14701 tcattgtttt tctcttgatt cccactttgt ggttctaagt actgtggttt ccaaatgtgt
    14761 cagtttcata gcctgaagaa cgagatcagc agcctctgtt ccacatacac ttcattctca
    14821 gtattgtttt gccaagttct aattccatca gaagctggtc gagtaacttt aaataattgg
    14881 cattatttaa agttaggcgc gccataactt cgtatagcat acattatacg aagttatgtc
    14941 gagatattta aatattagac ccagctttct tgtacaaagt ggttgatatc tctatagtcg
    15001 cagtaggcgg ctatataaaa tttactcccc atttctttgt gaatagcaaa taatcacatc
    15061 tcactctact gctttccaaa cacaatctta gaattttatt taaatgtcat atttaaatgt
    15121 gcctaagaga aagggcatgc tagtagtcat gctttctttc ttgtcattct tttcagtaaa
    15181 gcatgcaact taagtgagtc attaagctgt ttcccttgga gcgttagtga ccataatatc
    15241 tcacaattaa caaaacaagt gtgaacttaa ggagcttcta gactccgcag ctctgtgcga
    15301 tgatgttttg gaaatggtgc cagttcatct gaaatgaacc acattttgtt ttgctttcac
    15361 agtcgcagat atggccaata caccataaac caagaaggct ccaccaaagt tcccgagagg
    15421 cctccatttg atcgatcgag ttctcaggat tccttggatg aatcaatgga agcctattgg
    15481 acagaactag aaaacatcaa gaggtcgaat gaaaatcgcc aagaaggtca agaggccata
    15541 gttgtcaaag aacctgatgg taatgtctat gtgaataaga tatgtcttta tcagtagaaa
    15601 tgggtgtcca gaaaggaagg aacagacccc aaataacact gctggttgcc tttgaactta
    15661 aaggttactg gataatagca gataaaagag tccaaatacc aggtacacgt gttgtacctg
    15721 gtacaagcat agccatttta ttagagattc agtgagttag acttgagatg gcgcaacgca
    15781 attaatgata acttcgtata gcatacatta tacgaagtta tggtctgagc tcgccatcag
    15841 ttcaataata tattagcctc aaggctatct catctccagg aagtgtgaat gggtgatggt
    15901 aagcttccaa cttcgcagtg attcagtttt tccatttgtc aagtagcttg atttgttata
    15961 actatgttgt ttggaagtct gttttcttct atctcagagt gtgttgctgt aatctttctc
    16021 ctttaaggga gagccaatac gtttattcta cttttccata tatggtagtc ttttcaacat
    16081 tcgagggtgc tccttttgtt tctagccaat ccatttctag ataggtatga gcaacctgtg
    16141 tgtgtgtggt tttcttgacc tccgctagat ctggtcctct cttgttcagc ccagtatcca
    16201 gacagtctgc tttgccaatg gagaggttca gctttggatg ataaaagaca taagcatttt
    16261 cagttccctt agttccaatg ctcccttagc tgctgctttc tggtggttaa tgcattgttt
    16321 ttcttactgt tcctcctaac agtcttctat gtgcacttga tcattatatt tggtattcta
    16381 aagtaaacat gcaacacttc actttttaat atttttctta atttttgtac tatttctttt
    16441 aatcttttaa attcatgaca acaccatatt catgaagccc gatttgtcct taacattgtg
    16501 gctttctttc tgggctattc ataaatgttt atgtacattt tcgtgtccca gtagttacat
    16561 aaatgccaaa aggaaaaaaa ataaagtcat tatcagttct gtgatccatt agaatacaca
    16621 cccttaactc tcacctcagt gtctgagtta agtgtcccta taagtagtat tttcttcttt
    16681 tctatgatat tgctttaaaa atctgccagt attgatctgg agcctggctt ctgtgctctt
    16741 gttcaacaga gtctcactat gtagaacaca attgttttaa tttttttaat ccgcctgctt
    16801 catctttcaa agagctggga ttttaggtgt ttgtcaccat accaggcttg gatatttttc
    16861 tcaatttaat atttctcaga ccgataaaag aaaaccatat tttattaatt tgatgtctca
    16921 tctattttct cctatacttt tctacataca ttataagtaa aacttatctt atttgtcata
    16981 attattatcg tctttcagtc tgtgtttcaa gacagaagta acttgccagt tggtcagtag
    17041 gaaacaatat cttagggaaa accacactta agctttggct gtttgataat tctccaaact
    17101 taagttgaat taacagatga taaagaccaa tggacataac tctgtatact catatttttg
    17161 ttcctcctcc tcctcctctt tctgctcctc ctcctcctcc tgacgctgct gcttcttctt
    17221 ttcatctttc ttcttcttct tcttcttctt cttcttcttc ttcttcttct tcttcttctt
    17281 cttcttcttc ttcttcttct tcttcttctt catcgtcttc atcgtcttca tcgtcttcat
    17341 cgtcttcttc gtgtacttcg tcttctttgt cttcttttcc tttccttcct cctcttcctc
    17401 cccctcctcc ccctccttct ttgagatgat ctcctgaact cactatatag ctcagattgg
    17461 cctcccactt atagagatat atgtgccctt acctcctgtg ctgggattaa aggcaggaac
    17521 cacagtgccc acctcttcct agattttaaa tagtttgagt tttccttcta atttcaatgt
    17581 actaagtggg cataacaata ttgttttcct tgtgctgaca atgtcaaact ccttcctcac
    17641 catcactctg ggttggcaca tctggagtat tgtttagcct gtctattcat caataatcac
    17701 aatgcccagc tgttttcttt gaggcccagg ctattgatgc ttgatttcta cattcaagga
    17761 gttactcaag tgcagaagga cagacagcag tcatgagtgg cacagatgag aacatttgca
    17821 aaatatcatg atatttggtc aggaagtagc tctgatagct ctcatttagt tcatacgaaa
    17881 aaaggcagaa ttatatttag tgacagttct gaaatggggt atagacattt ttgtgtcctt
    17941 tgttacctgt ccttttttat ttctttctaa acatggtact tgaggtgact ctattctagt
    18001 tttccatctg tttttctata agcttcatta gatcttagat aatgtagaat cacttgatat
    18061 acaggaaatg attggttcca ggacctctag agcctagagc atccaaatca acagatagat
    18121 actaaaatgt gttgtatgaa tttgcatcaa cctatttatg tactcctata ttttgaaacc
    18181 atcacatatc atttagagtg tgtgatgcca tgcgcaaact attatataga tagttgtgat
    18241 attgtgttta ggtaggtcat aaaggagtct gtaacagggg gctccaaaac ttagagttct
    18301 gcctgagcta catagtgaga ctctgtctag aaagaaagaa agaaagaaag aaagaaagaa
    18361 agaaagaaag aggaagagag aaagaaaaag aaaggaagga aggaagagat gaagaaagga
    18421 aggaaggaag gaaggaagga agagaggaag gaaggaagga aggaaggaag gaaggaagga
    18481 agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa agaaaaggga ggaaggaaga
    18541 aatggaaaaa tagttgatac ctatgttcag atcaggcact acttttagca gcacatcgaa
    18601 tctaggttac ttgaatcttc atatgtggag accacagaca tggaaggcaa ctataattta
    18661 taattgaatt cctacttttt ttgttttcac ttgattccgt tatctattta gctttcaggc
    18721 acacacactc tctctctttt tttctttgaa aataatgtgc tttaaaaatt cttgaatatc
    18781 tatattatct gatgcacaca tttgagaaaa aaaaatagaa actttaattt gaaataccct
    18841 gttacttttt ttttttttct tcactacctt gccatatgtc tcattagcta catggaactg
    18901 agaaaacaaa cccttctgga aataggtgtt gggctccaag atagccttga actcaagcca
    18961 agaaaccctg cctccatgca ggcttgggac accaacctct gcgtcctccg ctgggatgtg
    19021 ttatttctcc atgcttgctc acctctagga ggacgacggg acagccttcc ctagttaata
    19081 tgcaggaact tgtagaggtt aattacatgt gaaacaattt tagaattttt aattaagtat
    19141 atatgaaatt gacccaaaat ttgctatcac tatggccagg gagaatggca gttgtccaag
    19201 tttcgaataa taattctcag ttctgttcgt ggcttagtta taacagaagc acttcagtca
    19261 ggatttcgga tagtctaaaa atattaacgt ttttattctt tgtgatttca aaaccatttc
    19321 tttcttattt ttttaaactt attttagagg gagaactgga agaagaatgg ctcaaagaag
    19381 caggtctatc caatcttttt ggagagtcta ttgatgaccc gcaggaaagc attctgtttt
    19441 tatctacact gacccggact caggcagcag ctgttcagaa acgggtggaa acggtctccc
    19501 agaccttgag gaagaaaaac aaacagcacc acattcgtga cgtcagagac atatttgctc
    19561 aacagagaga ggcacaagaa aaagtaagat tttttttttg atatgaaagc aaatgatgaa
    19621 atgcttagag tcctttggac cagtccagtt taaatcaagt cattacagac tttatgagca
    19681 cctgttctct acccagtgtt actgtgttgg cagtgaacca gtgttcttga gtttcgccct
    19741 tttagaagtt accccagaga gacaaatacc taagcaagtt caaattccta gcaatagttc
    19801 tgtactaggg cgcatggggt ccttggagta gatgtgcctg tcacactagt gagtgggaga
    19861 gagtcgtgaa gaagaccgtc tacacagctt ggtgccagtt gtttctgata gaaaagcagg
    19921 atccagggca cttagtcctt atcagttatt gcttgaggaa atcattgaat ttccaattca
    19981 ctgaaagcca ggtagaagtg gagtatcagg gaagtgatag caacccagga gctgggccct
    20041 tgagatgtga aggattatgc tctttgtgtt tagtcccctg caccttatat ggcatataga
    20101 tctgaattaa catcaaccca aatggttagt gttttcatgg atgatgctga ttgtgatgga
    20161 aatagaagag tgttaaatga agagacttta ggagataata gaagtactag aactgcgagg
    20221 ccctgaacag tgctgtgcac atagtccatt gatagggcac ccaaagttta tctataagga
    20281 aataaggagc ttgcattgga ttgaaatcat catatggaac ttttttcgaa caggttttgc
    20341 taccaacaag aacaaatcca ctgaactaaa tgatattttt aagagcacag atgattttgc
    20401 ttactgcccc cctcagctct ggtttagaat attcagtaga atcattggaa aggattcttt
    20461 aataatggtt caataatgac aacattaaca gtagcaattg ctaccatggc tactgagcta
    20521 actgctctac ctgtttgagt taattgaatc cccacagcta tcttctgagt gaggtgctat
    20581 catggccatc ctatggttga ggttcataga ctttaagtaa cttgctccat gtcctgtaga
    20641 tgctaagccc aattttgtct gactttaaag cattttctct tctgcagcat ttccactttc
    20701 aaacatgact ctgatagtaa gcagtttgaa cgtgagaatc gatttacagt acaagcaaag
    20761 ggcagtaaaa atgttatggc ttttcataaa aagcagaatg gaggtgctta tatagaagtc
    20821 actttgttgt ttttgataat gttatgtaga gagcctttac t
//