LOCUS       44849#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20471 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 44849
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000028984
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            5987..6313
                     /db_xref="ENSEMBL:ENSMUSE00000681404"
                     /rank="1"
                     /note="ENSMUSE00000681404"
     misc_feature    4275..8799
                     /note="5 arm"
     misc_feature    8827..8851
                     /note="B1 Gateway"
     misc_feature    8864..8911
                     /note="Frt"
     misc_feature    8912..9411
                     /note="mouse En2 intron"
     primer          complement(8980..9001)
                     /note="LRPCR LAR3_EN2"
     primer          complement(8876..8899)
                     /note="FRT3"
     primer          13077..13095
                     /note="seq primer NF"
     misc_feature    13137..13144
                     /note="RE sites"
     misc_feature    14937..14970
                     /note="loxP"
     misc_feature    complement(13145..13176)
                     /note="Rox"
     misc_feature    complement(14897..14928)
                     /note="Rox"
     misc_feature    13177..13198
                     /note="polylinker"
     CDS             13757..14353
                     /note="puromycin N-acetyl transferase"
     misc_feature    13872..13877
                     /note="Mutated RsrII site"
     intron          9412..9786
                     /note="mouse En2 intron"
     misc_feature    9784..9786
                     /note="Splice Acceptor"
     exon            9787..9840
                     /note="mouse En2 exon"
     primer          9687..9708
                     /note="seq primer FCHK2"
     misc_feature    9841..9963
                     /note="1Dleader"
     misc_feature    9964..10015
                     /note="F2A"
     misc_feature    10111..10827
                     /note="EGFP"
     misc_feature    10021..10110
                     /note="3X NLS"
     misc_feature    12893..13131
                     /note="SV40 polyadenylation site"
     misc_feature    11926..12870
                     /note="ERT2"
     promoter        13209..13720
                     /note="PGK promoter"
     misc_feature    14464..14896
                     /note="PGK pA"
     primer          12951..12974
                     /note="LRPCR RAF5"
     misc_feature    10837..10890
                     /note="T2A"
     misc_feature    10887..10887
                     /note="A to G to kill RsRII site"
     misc_feature    10891..11916
                     /note="Cre"
     misc_feature    9802..9841
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    9802..9817
                     /note="FCHK QC prefix region"
     misc_feature    9818..9835
                     /note="FCHK QC critical region"
     misc_feature    9836..9841
                     /note="FCHK QC suffix region"
     misc_feature    14993..15013
                     /note="B2 Gateway"
     misc_feature    8800..15043
                     /note="Synthetic Cassette"
     exon            15442..15592
                     /db_xref="ENSEMBL:ENSMUSE00000169974"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000169974"
     misc_feature    15044..15743
                     /note="Critical Region"
     misc_feature    15767..15800
                     /note="Downstream LoxP"
     misc_feature    15744..15823
                     /note="synthetic loxP region"
     exon            17318..17425
                     /db_xref="ENSEMBL:ENSMUSE00000169977"
                     /rank="3"
                     /note="ENSMUSE00000169977"
     exon            18062..18211
                     /db_xref="ENSEMBL:ENSMUSE00000169975"
                     /rank="4"
                     /note="ENSMUSE00000169975"
     exon            18911..18995
                     /db_xref="ENSEMBL:ENSMUSE00001234777"
                     /rank="5"
                     /note="ENSMUSE00001234777"
     exon            19374..19437
                     /db_xref="ENSEMBL:ENSMUSE00000274792"
                     /rank="6"
                     /note="ENSMUSE00000274792"
     exon            19931..20152
                     /db_xref="ENSEMBL:ENSMUSE00000274788"
                     /rank="7"
                     /note="ENSMUSE00000274788"
     misc_feature    15824..20471
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggactacgg tcaattatta tttaacatct agtgcctgat ttttttataa
     4321 atcttcaatg tataaattta aactatttta attctttcta attaaggctt tcgttaccat
     4381 ataccaaaat ccacttccaa tgatacatgt gtagccatat gaaattttat tgttgggaaa
     4441 gtttttatct atcataatag ttttgtaagt gatttagaag gtaaagagtt ggtttccctg
     4501 gggataaggt catgtcagtg gccctatctc tagggatggt ttcttaccca ttattctcac
     4561 ttaagatctt ggcctaggct accagggaca tgtaaataag aaaagaaata agagaaaaca
     4621 aaacagatag attgccatga gaactacgga tcaatatttt ttttctccag caaagagttc
     4681 cacaaccggt tccagaagac ctcccccttt tgaggtcaat tgcctcagtc tgtggaactt
     4741 gtcacataga tcctactgga ggtggtgtgg caatgcacct atatctgtcc ctcccagggg
     4801 ctcagggaac attacaaaag acaaaaagga cataagatcc agaagatgag gagaagtgct
     4861 atgaaattct atcttctggg catgacatgg ccattgtact catgaaccca cagcagctgt
     4921 gcttgcctgc acaaagatca aatcagctga aattccagca tagagaatgg aggagtccat
     4981 gaggccctgt ccttagctga gatagctgat ggcttctaaa agaaggagag tcctcttcct
     5041 ttgtggctgt ggctgagaga aggttgccca cggtctaatg gatggttccg cactcatgtg
     5101 catattggca ccgctaactg aacccaatgg gtaataaaaa aaaaaacctt aatattggga
     5161 gcaggatgtg ttgtgggacc tggaggaaga tggagagagt aaagggcaga taacaatcaa
     5221 aatctgctga atatgtgtgt gaaatattca aagaataaac acaaataata tattaaaaat
     5281 aaagttgaaa gatatcaaat gttagagaga acactgagcc cattgctttg ctgatcgggg
     5341 atgtaaattg gtcatatgga aaacaacata ggaggtacct caaaaagtta aaccaccaca
     5401 cgatctagca atcctgcttc tgggcatata ggggaaataa catcccacct cagagagaca
     5461 cctgtccttc tgcgctcact gaagcagtct tcatattgac tgcagtgttt atcaggaggt
     5521 gaatggaatg ttattaagcc ttggcaaagg agaaaaaatg ccaccatttc tgatggtgcg
     5581 aatggaccag caggctctta tgctatgtga aacaagccag atgctggcag atctcaagtg
     5641 tgagctcact ggaaagtagg atctaaaaga gtaaacagca gaaaaacaga gtagagcagg
     5701 gcttgctaca gggagataca ggagaggaga tggggttcca gggatacaac cttgcagtta
     5761 gaagatgaat aaatcctgga aacctcccca actcccagaa atgaaggaat aagaaagttc
     5821 tattccaatt ggaccttgct gtgttggtca tcctcactga agagtcacta gcctgtagct
     5881 tatgcccagg atcagggaga ggactcctgt tggatgaccc aacagtagct ctggtgagtg
     5941 tctccaagca accaagcctg cctctgcagg cctgaccttt gcatctgctg tccatgtgag
     6001 ctaaccctgg gagcagctac cagagccatt tgcaccacct cccacagcat gctggggaca
     6061 gctgtgaagc cacctgtcca aagacagctg taagcccatc gatctgggtg gccatactca
     6121 cagccaactg tctgggccct gctggaccat tactgacacc tgcaggttcg ggtgccagca
     6181 cctgagatgc atctactctg gaggctcctg gttctcctcc tcggattgct agccttgccc
     6241 tcagccctgc ccaagcagcc ttggcctgga ctgaccaagg ctcacaagga tggccggtcc
     6301 accctagcga gaagtgagct taccaccggg cttggcatcc tatccatagg gtgtggggag
     6361 ggcacagcct gggccccacc actcactgct gagcctctta tttatgtatt tatttattta
     6421 tttatccctt ggatcttaag tataggaagc tgagactggg ttcaattttt ccaattcata
     6481 gcacgtgatc ttttgtgtca ttttcttttt ctctttatct tcattcccat ccctcttaca
     6541 gccttggcag gaactgctgt gtgcttagca tgtgtttcta actatgtctg tatccagctg
     6601 ggcatggtgg catgtatgcc tttaaaacca gtacctggga gtcagaaaca ggcaagtttc
     6661 taaatttaaa gccagactgg ttttatattg tgagactctt tctcaaaaca acaacaaccc
     6721 acgtctgcat tctttgcagg aggtattcat acaactgact tgtcgctcac tctgcttttc
     6781 aggatgaaga tgcctgcttt ataccgctgg catcttgctt gacttgatga gtgcaaagaa
     6841 gtccatacct tctgttacct ccatttaatt ggctgctctc tcaggactga catccagact
     6901 gtctgtatct tcccgttgct ctaagcaacc ctgagaggag catccttgag aggagcatcc
     6961 ttgagaggag catccttgag aggagcatcc ttgagaggag catccttgag aggagcatcc
     7021 ttgagagtgt cccaattaaa acacatggga gaattcctct gggatgcata tatccagaac
     7081 agatgtaagc tctgtttagt tcaacctgtt ggactcctct taaaatggct gtgctggtgt
     7141 acactcattc cagttgtaag agaacatagc tgtctcccat gctcccctac catgggaggg
     7201 tatccacctg tgccaactag ccagtgctgc caaagcaaaa tgccataggt ggggtaggat
     7261 tggagtggtg gtagcggaac gtaaacaaca gatgggctgt tttttttttt ttaatgtagt
     7321 ctgttagtta gaaattcaag ttaaaggtgt tgacaggttt ggtttctggg gagggacctc
     7381 tcttgctggt ttgtggatgg cactttctca cagtctgtca gaaaaggaca tgcctgatat
     7441 cttcctctta caaagacacc tgttcaattg gatgaggact gaccctgtga actcatttaa
     7501 acatatttgc tgccttgaag ggactatctc aaatacagtt gcacctagat acccatgggg
     7561 attggttcca ggttccccat cgggtcctag gatatttgga tgcaccaacc cttttcataa
     7621 aatgatacag tgtctgcaca gaactgatgc aagtccttcc tcatacctga aaacatcttt
     7681 agattacttg taagatctgg gacaatgcga atgacttata aataactgtt gcagctcact
     7741 cagggaaaaa tggcaagaaa aaaatggaca tgttcagaac acatgtaatt tttttccaaa
     7801 tatttccaat ccacagttga ctgtgtagac aaaacagaag atggccaatg ctcctcccaa
     7861 atggcaccta ttgactttgc caaacaggac tagactccaa tacatcaata tatcattata
     7921 tagcttgtat tttgagtcct tttatgatgg aggagaaggt ttgctataga tatatgacag
     7981 aacatagcca gaggcagaga cagagacatc taagacagtt cagagtggcc atgatcagac
     8041 tgatctgtgt tggggagggg gggtggcgtg gagagccagg tgcaggcagg caggaggccc
     8101 aagatcttgt cacaccccag aattactcta gttgtctatt tcaacagtgt tctagtttta
     8161 ctttccactc tgagggtgtc tatacacaca gtggactttt atctctatgt gcatctgtgc
     8221 atgaaaagtg aaacccagcc tcattcctgc ccacatgagt cagtttccca acccatctca
     8281 taaccgacta cattcccaac ctgaacactc gcctgctttc agacctgtct ctgcaacata
     8341 atactttagt tgttgcttca ttgcagaata ctgcttcttc tgaccaccat tttattcttc
     8401 cccatgaact ttccagtatg ttgagttcct ctgaaagaca tctgatttca gtcacattga
     8461 atctgcctgt atatttagag gaaatgaatt atgttcacaa tggaagaccc tggccatagg
     8521 ctaaacatgt tgttaatcag actttacact atgcctcata aaatcctatg gaagttttga
     8581 gcatggtttc tcaaggtgat cccttgagaa tttatggggt ttttttctgc agagtaaatt
     8641 attttaatat attcctagtt ggtttttttt tttttttttt tttttggtac aactgggaga
     8701 gatcttcagc tatagcttcc acttaggaaa cattttaata tgggtctgtt tcttctctat
     8761 gggagacatg tatcttagct ggggttttat tgctgtgaaa aggcgcataa cgataccacg
     8821 atatcaacaa gtttgtacaa aaaagcaggc tggcgccgga accgaagttc ctattccgaa
     8881 gttcctattc tctagaaagt ataggaactt cgaacccttt cccacaccac cctccacact
     8941 tgccccaaac actgccaact atgtaggagg aaggggttgg gactaacaga agaacccgtt
     9001 gtggggaagc tgttgggagg gtcactttat gttcttgccc aaggtcagtt gggtggcctg
     9061 cttctgatga ggtggtccca aggtctgggg tagaaggtga gagggacagg ccaccaaggt
     9121 cagccccccc cccctatccc ataggagcca ggtccctctc ctggacagga agactgaagg
     9181 ggagatgcca gagactcagt gaagcctggg gtaccctatt ggagtccttc aaggaaacaa
     9241 acttggcctc accaggcctc agccttggct cctcctggga actctactgc ccttgggatc
     9301 cccttgtagt tgtgggttac ataggaaggg ggacgggatt ccccttgact ggctagccta
     9361 ctcttttctt cagtcttctc catctcctct cacctgtctc tcgacccttt ccctaggata
     9421 gacttggaaa aagataaggg gagaaaacaa atgcaaacga ggccagaaag attttggctg
     9481 ggcattcctt ccgctagctt ttattgggat cccctagttt gtgataggcc ttttagctac
     9541 atctgccaat ccatctcatt ttcacacaca cacacaccac tttccttctg gtcagtgggc
     9601 acatgtccag cctcaagttt atatcaccac ccccaatgcc caacacttgt atggccttgg
     9661 gcgggtcatc ccccccccca cccccagtat ctgcaacctc aagctagctt gggtgcgttg
     9721 gttgtggata agtagctaga ctccagcaac cagtaacctc tgccctttct cctccatgac
     9781 aaccaggctc ccaggtcccg aaaaccaaag aagaagaacg cagatcgcag atcgcagatc
     9841 tctagagtca ccgagttgct ttaccggatg aagagggccg aaacatactg tccaaggccc
     9901 ttgctggcaa tccacccaac tgaagccaga cacaaacaga aaattgtggc accggtgaaa
     9961 cagactttga attttgacct tctcaagttg gcgggagacg tcgagtccaa ccccggaccg
    10021 gtgcatgtgg accccaagaa aaagcggaag gtggacccta aaaagaaacg caaagtggat
    10081 cctaagaaaa aaagaaaggt gcacgtgtcc atggtgtcca agggcgagga actgttcacc
    10141 ggcgtggtgc ccatcctggt ggaactggat ggcgacgtga acggccacaa gttcagcgtg
    10201 tccggcgagg gcgaaggcga cgccacatac ggaaagctga ccctgaagtt catctgcacc
    10261 accggcaagc tgcccgtgcc ttggcctacc ctcgtgacca cactgaccta cggcgtgcag
    10321 tgcttcagca gataccccga ccatatgaag cagcacgact tcttcaagag cgccatgccc
    10381 gagggctacg tgcaggaaag aaccatcttc tttaaggacg acggcaacta caagaccagg
    10441 gccgaagtga agttcgaggg cgacaccctc gtgaacagaa tcgagctgaa gggcatcgac
    10501 ttcaaagagg acggcaacat cctgggccac aagctggagt acaactacaa cagccacaac
    10561 gtgtacatca tggccgacaa gcagaaaaac ggcatcaaag tgaacttcaa gatccggcac
    10621 aacatcgagg acggctccgt gcagctggcc gaccactacc agcagaacac ccctatcggc
    10681 gacggccctg tgctgctgcc tgacaaccac tacctgagca cccagagcgc cctgagcaag
    10741 gaccccaacg agaagaggga ccacatggtg ctgctggaat tcgtgaccgc cgctggcatc
    10801 accctgggca tggacgagct gtacaagcct gcaggcgagg gcagaggaag tcttctaaca
    10861 tgcggtgacg tggaggagaa tcccgggccg tccaatttac tgaccgtaca ccaaaatttg
    10921 cctgcattac cggtcgatgc aacgagtgat gaggttcgca agaacctgat ggacatgttc
    10981 agggatcgcc aggcgttttc tgagcatacc tggaaaatgc ttctgtccgt ttgccggtcg
    11041 tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc ccgcagaacc tgaagatgtt
    11101 cgcgattatc ttctatatct tcaggcgcgc ggtctggcag taaaaactat ccagcaacat
    11161 ttgggccagc taaacatgct tcatcgtcgg tctgggctgc cacgaccaag tgacagcaat
    11221 gctgtttcac tggttatgcg gcggatccga aaagaaaacg ttgatgccgg tgaacgtgca
    11281 aaacaggctc tagcgttcga acgcactgat ttcgaccagg ttcgttcact catggaaaat
    11341 agcgaccgct gccaggatat aaggaatctg gcatttctgg ggattgctta taacaccctg
    11401 ttacgtatag ccgaaattgc caggatcagg gttaaagata tctcacgtac tgacggtggg
    11461 agaatgttaa tccatattgg cagaacgaaa acgctggtta gcaccgcagg tgtagagaag
    11521 gcacttagcc tgggggtaac taaactggtc gagcgatgga tttccgtctc tggtgtagct
    11581 gatgatccga ataactacct gttttgccgg gtcagaaaaa atggtgttgc cgcgccatct
    11641 gccaccagcc agctatcaac tcgcgccctg gaagggattt ttgaagcaac tcatcgattg
    11701 atttacggcg ctaaggatga ctctggtcag agatacctgg cctggtctgg acacagtgcc
    11761 cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt caataccgga gatcatgcaa
    11821 gctggtggct ggaccaatgt aaatattgtc atgaactata tccgtaacct ggatagtgaa
    11881 acaggggcaa tggtgcgcct gctggaagat ggcgatctcg agccatctgc tggagacatg
    11941 agagctgcca acctttggcc aagcccgctc atgatcaaac gctctaagaa gaacagcctg
    12001 gccttgtccc tgacggccga ccagatggtc agtgccttgt tggatgctga gccccccata
    12061 ctctattccg agtatgatcc taccagaccc ttcagtgaag cttcgatgat gggcttactg
    12121 accaacctgg cagacaggga gctggttcac atgatcaact gggcgaagag ggtgccaggc
    12181 tttgtggatt tgaccctcca tgatcaggtc caccttctag aatgtgcctg gctagagatc
    12241 ctgatgattg gtctcgtctg gcgctccatg gagcacccag tgaagctact gtttgctcct
    12301 aacttgctct tggacaggaa ccagggaaaa tgtgtagagg gcatggtgga gatcttcgac
    12361 atgctgctgg ctacatcatc tcggttccgc atgatgaatc tgcagggaga ggagtttgtg
    12421 tgcctcaaat ctattatttt gcttaattct ggagtgtaca catttctgtc cagcaccctg
    12481 aagtctctgg aagagaagga ccatatccac cgagtcctgg acaagatcac agacactttg
    12541 atccacctga tggccaaggc aggcctgacc ctgcagcagc agcaccagcg gctggcccag
    12601 ctcctcctca tcctctccca catcaggcac atgagtaaca aaggcatgga gcatctgtac
    12661 agcatgaagt gcaagaacgt ggtgcccctc tatgacctgc tgctggaggc ggcggacgcc
    12721 caccgcctac atgcgcccac tagccgtgga ggggcatccg tggaggagac ggaccaaagc
    12781 cacttggcca ctgcgggctc tacttcatcg cattccttgc aaaagtatta catcacgggg
    12841 gaggcagagg gtttccctgc cacagcttga tgaagatctg agctcttaat taatgatcat
    12901 aatcagccat atcacatctg tagaggtttt acttgcttta aaaaacctcc cacacctccc
    12961 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
    13021 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    13081 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgttt
    13141 aaactaactt taaataattg gcattattta aagttacata tggcggccgc cctaaggctc
    13201 gacctcgaaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
    13261 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
    13321 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
    13381 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
    13441 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
    13501 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
    13561 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
    13621 gggcgcccga aggtcttccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
    13681 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcatccatc tagatctcga
    13741 gcagctgaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
    13801 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
    13861 caccgtcgat ccagatcgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
    13921 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
    13981 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
    14041 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
    14101 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
    14161 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
    14221 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
    14281 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
    14341 caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg
    14401 accccatgca tcgatgatat cagatccccg ggatgcagaa attgatgatc tattaaacaa
    14461 taaagatgtc cactaaaatg gaagtttttc ctgtcatact ttgttaagaa gggtgagaac
    14521 agagtaccta cattttgaat ggaaggattg gagctacggg ggtgggggtg gggtgggatt
    14581 agataaatgc ntgctcttta ctgaaggctc tttactattg ctttatgata atgtttcata
    14641 gttggatatc ataatttaaa caagcaaaac caaattaagg gccagctcat tcctcccact
    14701 catgatctat agatctatag atctctcgtg ggatcattgt ttttctcttg attcccactt
    14761 tgtggttcta agtactgtgg tttccaaatg tgtcagtttc atagcctgaa gaacgagatc
    14821 agcagcctct gttccacata cacttcattc tcagtattgt tttgccaagt tctaattcca
    14881 tcagaagctg gtcgagtaac tttaaataat tggcattatt taaagttagg cgcgccataa
    14941 cttcgtatag catacattat acgaagttat gtcgagatat ttaaatatta gacccagctt
    15001 tcttgtacaa agtggttgat atctctatag tcgcagtagg cgggaaagta tggcagtgtc
    15061 caggcagaca tagtgctgga gttgctaaaa gttcttcatc ttgatcagaa ggcaacagga
    15121 gactgtcatc tgtgggcagc caggacaaga ctcggattct acactgggtg gaacttgagc
    15181 ataggagacc tcaaaccctg cccccatgat gaagcacttc ctccaacaag gccacaccta
    15241 ctccaataag gtcactctct atggccaagc attcaaacac atgagtctat ggaaaccaaa
    15301 tctattcaaa tcaccacaac atagaaacat gaataagtgc tcaacagaaa agggatacgc
    15361 tgggttcgtc ccaggcctgc tgcctctgcc ctccactctt aaacaacaat atgcacatca
    15421 ccatctcttc tccaattcca gttattgcac agggcctcct gaagctcaat gcagaaggac
    15481 ggatccagag catgcgcctt ctggaccgtc tgaacgtctc agggacagtg gctccaggga
    15541 tggtgggctg gctgattggt ggcatgaact tccagcagca acaagaaatc aggtaagacc
    15601 ttgactccta ctacagactc tgggggaaac tctagggatg gtcagagggc agaatgggca
    15661 aaacagagaa caagatttag gaagaaggaa ggcattcgga attaaggaag acaaggcaca
    15721 aagccaaaga tgagaacaat ctagagatgg cgcaacgcaa ttaatgataa cttcgtatag
    15781 catacattat acgaagttat ggtctgagct cgccatcagt tcaaaagctc acctttgctg
    15841 ctagctttag ggaaattgtt tggccattct aagctcacct gtaaaatggg gacacagagc
    15901 agagcctgga aaaaatatcc agaatatcag aggagaccaa ggatgcagcc agacacttga
    15961 ctctccattc aagtgggaca tagctcttgc ggctctgcgt aaagagtgga ctacattacc
    16021 cacaaacctt gggccctcaa cagataaaca gtcataggga tggtcccacc cacaggagat
    16081 gctgtggact ctgacagtcc tgcacagaac ttttatggtc aacacaaaag atacataacc
    16141 ctcattaact ggggttctca tggcagaact gggtacatgc tacccacagc cactgagact
    16201 gctcccttga cccttctgcc tgccctcaat ggttctgcct gctctgcccc caaaaatgtt
    16261 ctgccttttc tcttgacctg aattcctcat ttgtctctgt ttggataaac cacagtagtc
    16321 actatcagga tttttctcac cagcagggtt ttctggtgaa acatgcagcc caaatcacct
    16381 ctttttagtc attcctttag caaatgtcac aattatcacc aattatctac tgggtatcac
    16441 tcacattgag aataaagcta tgagcaagac ctatgacttt tgcctttggg aagcctccat
    16501 cccagtggac taggcctcag agagtaataa caaatttcag gtgaagggac atgccatgaa
    16561 aacaaatcta gcaatgtgag ggtgggagtg atggggctga ccaggacctg tccaagaggt
    16621 gagccttgaa tgaagagaaa agggcaaatg atgtgaggga tatgagacac agtttgggaa
    16681 gaagaggctg taggtgcaaa agccctgagg tagaaacgat atagatcatt gtgttcaaag
    16741 aagagagtgc cctccatatg ggtggagagg tagggaaacc aatatcataa ggcaaatgtg
    16801 tatagaccct gcaggtcatt gtgtacagtt tgtatcaggg taccaggggt ggtatgaagg
    16861 tcacccaggc agcctcatag agaatggtct aaagagaggc caagatcaca gcagtgagcc
    16921 caggaaggaa atgaagcatc ttcaggacga ggcagtatga gcagcagagg tagtcaaact
    16981 caggatatac attgcaaata ggccaaggta gtttgcagat aacaatttgg aggtatagtg
    17041 tgtgggaaag ccaaggagtt tccctgtggc ctcccagaca gatgataggg cctcagtctg
    17101 cgaagagcct cagagtcctc agatggccac tgtcaatgtc tggttctgta ctgcacaatc
    17161 cacagctgtg gggaaggctt caacagctgg ctgcccagac tgtcagaaca ttcaccccag
    17221 gctccaccac ctgaccctgc ttgcagacaa gactggcctt gtttctgagt tgtcctccct
    17281 catccttgtg gggggtctct gctttttcca cttacagcat caacattacc aatgtgcagc
    17341 tggactgtgg tgggatccag atggctttcc ccaaagagtg gttctctgca aacatcacac
    17401 ttgaatttga cattgaattc aaactgtgag tcccaccaaa ccaagcagcg tctttgggat
    17461 atttttcctc cttcttggga acccagaagc tcggggtgta gtgggggtgg cagacttgga
    17521 cgttcagacc tttcatgacc ccaacctgaa ctccaatcat gacctggaat gagtcactat
    17581 tccctgaact ttggtttctg atgctgcagt gaagatgata ctcagtggag gaggtcattt
    17641 gctcattcaa acccgtgtga agtcagcaac tgtgtcccat gaaagaaagg aggggttccg
    17701 ctctgactct tgtccctctg ctcattcccc tcctgctcag acttcctgta agggaaacag
    17761 gagctgcagc tgatctcctt atgtaatgtt caaaagtcac tctaagtctg ggagtgcaga
    17821 gtgggcacgg ttcagtggga gaggctgcac ttaacatatc gggctttaca ttccatctct
    17881 aacaccagaa aaagaaaatc aagcatagaa gtcactgagg tccatggcca gttcctcctt
    17941 cctaacctag ggcttagtgt gcgtaggctg ttaggaagat ggtgagaagt acctggcttt
    18001 gccacacttc ttgctggcca ccaccacttc catccacaca gtcgctccta tctttccaca
    18061 ggcccttcaa ttcgaatatc atcaaaacgc atgcctgcat gggcctcact gcagagtcct
    18121 ggctggagaa agatgagttt ggccggaggg agctggtgat gggcaggtgt cgaatggagc
    18181 ccagcagtgg gggtgcatcc atgtccactg agtaaggctt tctgctcccc caggacagcc
    18241 ctgcctcatg aacggctgcg gcttcaaatc cccacacttc ctaagtccaa agcagacttc
    18301 acctctttgc tgagactttc tcctcctgtc ctcccacccc agaagcctcc tccctatgtt
    18361 tcctgtattc accctgttat ccaccaactg cagaaatacc ttttgaaggt ggaactctgc
    18421 tctttcccct aaggtcctgc tccaaagcct ttgtggggag agggggtgga gtacaaattc
    18481 agaaatgctt tttgggcccc tctaccctca ctgctgcctg gctctgtggc catttttcct
    18541 ccattaacag gggtgtgctg atttctctct gtaatttcca aacgctgaac catgacactt
    18601 ttcatggatg cctgccctcg taagcatcag gtgtgtggta acaacttaga ctgtggcaga
    18661 cattgacagt gtaggggaag accatgaact ctggtgctct cctgccatgg tcctaataag
    18721 agttggcact tgttcctatg tgaccttggc catgtcacat cacctctctg ggtccccatt
    18781 gactgtcaca acaagaatac ttccctagat ggccagatag gagttcaaga gcatgttcag
    18841 cacatacttc ccagccttgg tgtagcctgg gttggttcac tctatcctga actcccacct
    18901 cttctcctag ggaaacctca ccgaagatga aacattttct ccataacctc agagaaagcc
    18961 tagggaaagt tatcccaaat ctggtagaaa gtcaggtaag tttaaggaaa ttgtcatccc
    19021 tgcagacatc tgtgagtctg aggatcgagg tacacatcta ggtaacttag cagacaactt
    19081 ctgccgctgg gctgtggaat ctgacagcaa atatcagtag ccagtggcaa gggacagcca
    19141 ccctaagtgt ctgtgttctc gtgtgtagca cagacaaagt tgaagtcccc acctcataga
    19201 ctcgtggggc ttcaagaggt agacatgggt gtactgtgca tgcctcctat ggttggctgg
    19261 gtcctgctgg aatccagcag tgttggtgcc cctcaccccc ccccccccca gcctcagaga
    19321 cctgaattct ttcttatccc tggctgacac tcttgtctgt gctgggggcc taggtctgtc
    19381 ctctgattgg tgagatcctc cggcagctgg atgtgaagct gctgaaaggc ctagtaggtg
    19441 agtgttgatg tctatcccag gagatcgcct tggaactggt agctactgag ggaggaacca
    19501 cagctttcat gtgctgtgag aacaaatgat aagtactcta ttgcaatgct cacccaccat
    19561 gtgagtgggg agaatagaag ggactgtagt caactagatt gtaccctacc aagtcaccag
    19621 actggagatt ctagcttccc ccaagagttc tgcacatcca gtagggtgga cattcccctg
    19681 tctaaactca agacaacccc tgcttcaggg acggttgaac ttctccagcc cccctgccct
    19741 ggtggatctc acagtcttgg aaatagagga caggaagtca tttgtcaagg gacatctttc
    19801 tgaacaagtg atgggaagag aatgttcctg gctccagccc ttggaggata gatggggaag
    19861 cagacccttc tctgtgaacc tggggattag aaggatcgcc tagcaaccaa gacatccttt
    19921 ttcctttcag aacaggtgcc tgctcataat ctgggtcaac cctgaatcag tcagatctct
    19981 gctgccagac tcaaggagcc tcccattgcc tgccattgat cagaaggagg tacttccgat
    20041 tgagacctta agtctccctc aatgtgtggc ttctgctgtc ccctggaaaa aaatgacccc
    20101 caggtcccaa aacatcctgt cctttccaac aataaactct aagtctgaag ggacatatca
    20161 acatcacctg aaggatacac cagcccaagc cccttacaac ccccactcca acatcccaga
    20221 ctcatttgct catctaggtt tagttgagca cttactgtgt gccatgtctg ttgctgggct
    20281 cctagggggt acatagaaag cataatagag cagaaatgca aaagagactc tgaatcagac
    20341 tagattcagc tctataaagg catgattcca tgagcaactg gtgtctccag ctccaaaaca
    20401 gagatagaat aggttcaggg taaaattagt gggtaactcc caacctgaat actcactatc
    20461 tgtgtcttag a
//