LOCUS       48591#pL1L2_GT2_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21040 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT2_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 48591
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000006353
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            5781..5950
                     /db_xref="ENSEMBL:ENSMUSE00001210252"
                     /rank="3"
                     /note="ENSMUSE00001210252"
     misc_feature    4275..9063
                     /note="5 arm"
     misc_feature    9091..9115
                     /note="B1 Gateway"
     misc_feature    9128..9175
                     /note="Frt"
     misc_feature    9176..9675
                     /note="mouse En2 intron"
     primer          complement(9244..9265)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9140..9163)
                     /note="FRT3"
     primer          13334..13352
                     /note="seq primer NF"
     misc_feature    13394..13401
                     /note="RE sites"
     misc_feature    15194..15227
                     /note="loxP"
     misc_feature    complement(13402..13433)
                     /note="Rox"
     misc_feature    complement(15154..15185)
                     /note="Rox"
     misc_feature    13434..13455
                     /note="polylinker"
     CDS             14014..14610
                     /note="puromycin N-acetyl transferase"
     misc_feature    14129..14134
                     /note="Mutated RsrII site"
     intron          9676..10050
                     /note="mouse En2 intron"
     misc_feature    10048..10050
                     /note="Splice Acceptor"
     exon            10051..10097
                     /note="mouse En2 exon"
     primer          9951..9972
                     /note="seq primer FCHK2"
     misc_feature    10098..10220
                     /note="1Dleader"
     misc_feature    10221..10272
                     /note="F2A"
     misc_feature    10368..11084
                     /note="EGFP"
     misc_feature    10278..10367
                     /note="3X NLS"
     misc_feature    13150..13388
                     /note="SV40 polyadenylation site"
     misc_feature    12183..13127
                     /note="ERT2"
     promoter        13466..13977
                     /note="PGK promoter"
     misc_feature    14721..15153
                     /note="PGK pA"
     primer          13208..13231
                     /note="LRPCR RAF5"
     misc_feature    11094..11147
                     /note="T2A"
     misc_feature    11144..11144
                     /note="A to G to kill RsRII site"
     misc_feature    11148..12173
                     /note="Cre"
     misc_feature    10066..10098
                     /note="FCHK lf2a_lacz_gt2"
     misc_feature    10066..10081
                     /note="FCHK QC prefix region"
     misc_feature    10082..10092
                     /note="FCHK QC critical region"
     misc_feature    10093..10098
                     /note="FCHK QC suffix region"
     misc_feature    15250..15270
                     /note="B2 Gateway"
     misc_feature    9064..15300
                     /note="Synthetic Cassette"
     exon            15654..15766
                     /db_xref="ENSEMBL:ENSMUSE00001232971"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00001232971"
     misc_feature    15301..15944
                     /note="Critical Region"
     misc_feature    15968..16001
                     /note="Downstream LoxP"
     misc_feature    15945..16024
                     /note="synthetic loxP region"
     misc_feature    16025..21040
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggagttcag ataacctaat gaggaaagtt aataggtaat agatgccata
     4321 gtatgcatgg gcctagactc tttctttact gccagcttca accccaattc attacttctt
     4381 tctttgaggt tattttattt tatatatatg aatattttgc atgcatgtac atatgtgcac
     4441 cacatacatg cctggtacct ttgaggttca gaaaagggta tcagattctc tggaactgga
     4501 atcatagatg gtattgagtc accatgtggg tgctgggaac agaacccagg tcctctgcta
     4561 gagccacagg ctttcttatc tgctaaacca tctctctatc cccttctcta agacttttat
     4621 cattatggca tgttggattt tgtcaaaggc atctattgag atgatcatga ggtttttttc
     4681 atctttaagt tcatttatgt catttattgg cttaatatat gctgaactat ctctacatct
     4741 ctgggataca gttaatttaa tgtttttgat atagacctac ctgtgtttgg tttgtaaaca
     4801 ttttattcat gattataaca ttagtgttta ccaggggtat tagctggtag ttttcttttt
     4861 ctttctttct tttttttttt ttttaatgtg tgtgccttta tttggttttg atattagagt
     4921 aatactttgt agttgtttac caatactagt tttgattggc atagctgtcc tatgtgtgtg
     4981 tgtgtgtgtg tgtgtgtgtg tgtgtgtgca tgcatgactc accatattat atgtatatga
     5041 gcgtttttgc ctgtatgtat ttatgtgcac caaaaatgtg cctggtgccc aaggaggtca
     5101 gaaggtgcca gatcccttga actggactta tgatggtatt gagcctctgt gtgggtactg
     5161 gaactgaacg atggacttct ataaaatcag caagttctct tatctgctga gccatctctt
     5221 gagccccacc aatcactctt ttttttgttt gtttgttttt ttgttttttt gttttgtttt
     5281 tcgagacagg gtttctctgt atagccttgg ctatcctgga gctcactttg tagaccaggc
     5341 tggcctcgaa ctcagaaatc cgcctgcctc tgcctccgga gtgctgggat taaaggtgtg
     5401 cgccaccacc gcccagcccc accaatcact cttaaagtca ggtataccaa ttccttctca
     5461 cagtactgga ggccttggtg gtcactggcc tcagcagcag tggctgatgg gaattgctgg
     5521 ctttctgttt cctttctctt gaaaagaccc tgccctactt tctctccata gtattagtta
     5581 tgacattttc agttcagtca tgggtgttct atatatgcag gacttgcatg gttgtttctt
     5641 tggtttatct atgttttctg tggacttagg tttcacagag ttaagaagat tcaaggttgg
     5701 taagaattgt tgaccaatac catgctaagt ggatttttat aaacctctgc tatttgtaac
     5761 ttttttctgt cctacaaaag atgccttctg cgtcttgtga tgtactgctg gatgacatag
     5821 aagatatcat atcacaggaa gattcaaagc cacaggacag acagttctca agaaagcatg
     5881 tgtttcctaa agtacgaagg cggaataccc aaaaatattt gcaagaagag cccaggccac
     5941 caagtgacag gtaagcattc ctcagaatta cattcacttc cgtcccagaa gaggctggca
     6001 ctgctggcct acactgtatg tgcagtaact ccaggtatag gatctttgta tttctagtag
     6061 gaaacataga gcttaaaaaa aaaaaaaaaa aaaaaaaagg aattgcttca aatctggcca
     6121 gtaataggtt aaaaactcag aaatttctaa ttcttcatac caagggcatg gggaattagg
     6181 ggaacggata tgatcaaatt acattgaaga gaattttcca agaattaatt aaaaacaaaa
     6241 caaaatacca gtttaaatgt tctttgaagg aaggtgatag gacactagga gttgttttga
     6301 tttgactata gttggtataa atctgttgta attgcagatt aaagctttga atcctctgtg
     6361 gcagtgggtg ttttggacgt gtgattattg ttagcatatt agtgagtgct tagggatcac
     6421 acacaaggca ctgggcacaa agaccattgt cctgtctgcc cttttgtggt gggattctag
     6481 acagtaagag tgtagtagga cactgcagag gtgattgcct agctgtccat agcctgtgac
     6541 tgttcaccta gtaaatggtg ggcagctctc atgttggctt tgtttagatg attgagcttt
     6601 tcagggctct gcatggagaa ggtggttttt tatttttatt tatttatttt attagatatt
     6661 ttcttcattt acatttcaaa tgctatcccc aaagtcccct ataccctcct cccgccctgg
     6721 tagaaggtgt tttaattagt ggagtttgtc acatttattt caagcatgtc tttattgtca
     6781 tttctgttta cttttaagtt gttaggacag tctaagagga gagtcttttt gtccttccga
     6841 gataaattct ctagaaacct gtgactggag ttacatgaat tatggagttt tcaagtttgg
     6901 ttatttttaa tctgtaaatt ttaagtggat aaagattgca actagtattt aagagtttgt
     6961 gaatggacag ttcatcagcc tttgttttta tttatttatt tatttttatt tatttatttt
     7021 ctgagacagg gtttctctgt gtagccctgg ctgtcctgga actcactttg tagaccaggc
     7081 tggcctcgaa ctcagaaatc cgcctgcctc tgcctcccaa gtgctgggat taaaggcgtg
     7141 cgccaccact gcctggctgt ttttattctt aatatgtttt ctgtgttgta tactctgtaa
     7201 atgttcatga gagtgaacag gtacctgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     7261 gtgtgtgtgt gtgtgtacac tacaggtcag attaggtatc ttccttgatt gctttccacc
     7321 ttatttcttg ggacagggac agggacaggg actgtcactg aagctggagc tgttgattct
     7381 gctagggtgg ctgcctgttt gttctaggga ttcttctgtc ttctccctga caggcactgg
     7441 ggttgcatat gcctgctgct ccacctgact ttttttctgg gtgctgggtg tccagtaccc
     7501 ccaggtcata tcctcatact tatagggtag tactttatgg catcaccgtc tcctcagccc
     7561 ttcttgtttt gggtgctaca gctgccattt tcggtgtgac cgtttgtgtg tatctttcta
     7621 aaagctgcat cagtcattgg tggtatggtg agtagggatt aaaactacaa tccagagaag
     7681 acctttttct gaggcaagat ctcttgtagc ttggtccaca gatgaccctg aacttcctgc
     7741 ttctctttcc taagtgctgg gattacagtt gtgtgccacc tggcttgaaa ggacattttt
     7801 aaaattaaaa tttgtattta aaatgttttt caaaaatgac atcttattta cttaggatgt
     7861 atgagcaggc atgagcgcgt gctgaagcac gtgagtgagg gtcagaagtc agcttaggag
     7921 tcggttctct aacgtggatc tcggggttgg actcaggtca tcaggcgtgg gggcaggctc
     7981 cgtgacctgc taagccttct catggatcca aaaggacgtt atttatttac ttctaacatc
     8041 attttcacct tctattgaag atagattttg ttcaacataa ttttctgatt atgatctctc
     8101 cttcctctac tcctcccagc tcctatccat ctcccctccc atccagatcc accccttttc
     8161 tgtctctctc agaaaacaaa cagaataaaa taaaatgtaa taagagaaaa cagaaaggca
     8221 ttgacctatg actatagtgt caatttattg ctacatttgt ttggttttac cctagtgtct
     8281 ggcctatcca gtctggccca acattgttgg ggatgtgttc tgtctcatgg agtgggccat
     8341 aaatccaatc agatattagc tggcatgtct cacaagcctt atgctactgt tgcactagtg
     8401 cagtgattct ctgtcttcct aataatgtga ccctttaata cagttcctta tgttgtggtg
     8461 accctcaact atagaattac tgcattgcta cttcataact gtaattctgt tatagctatg
     8521 aattgtaatg taaatatctg acatggggga tatgcagcct ccgaaggggt catgactggt
     8581 gggttgagaa gctccgaagc actgcactag tgcatctggt atggaggtca ccattgtagt
     8641 ctgaagagtt tgcagctggg ttagtattta ccctctcctc tagtagtctg cagagtacat
     8701 ttcagtactg tgaccattta gtcagtagtg gtgaaggctc ttggtaggca acaactgcac
     8761 ttctccatgt tcagtgagat gtgtaggtgt tgtctttagc agtagggcct taccataagc
     8821 gtatggagag caatcttatc aacagcctgg gttgttttgg ggttcccatg ggacccttag
     8881 accaacaact cagttagagg taatctattt ctagtcctgg catttggtga caaaagacac
     8941 aaaaggacat tgtataaaga cagagagcct tcttgtgact ccttggttat tttatccgga
     9001 ttttagtttt aaaacacttt gcttattatt gtgtctgtat gcagaatgtg tttaagtctt
     9061 aggaaggcgc ataacgatac cacgatatca acaagtttgt acaaaaaagc aggctggcgc
     9121 cggaaccgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcgaacc
     9181 ctttcccaca ccaccctcca cacttgcccc aaacactgcc aactatgtag gaggaagggg
     9241 ttgggactaa cagaagaacc cgttgtgggg aagctgttgg gagggtcact ttatgttctt
     9301 gcccaaggtc agttgggtgg cctgcttctg atgaggtggt cccaaggtct ggggtagaag
     9361 gtgagaggga caggccacca aggtcagccc ccccccccta tcccatagga gccaggtccc
     9421 tctcctggac aggaagactg aaggggagat gccagagact cagtgaagcc tggggtaccc
     9481 tattggagtc cttcaaggaa acaaacttgg cctcaccagg cctcagcctt ggctcctcct
     9541 gggaactcta ctgcccttgg gatccccttg tagttgtggg ttacatagga agggggacgg
     9601 gattcccctt gactggctag cctactcttt tcttcagtct tctccatctc ctctcacctg
     9661 tctctcgacc ctttccctag gatagacttg gaaaaagata aggggagaaa acaaatgcaa
     9721 acgaggccag aaagattttg gctgggcatt ccttccgcta gcttttattg ggatccccta
     9781 gtttgtgata ggccttttag ctacatctgc caatccatct cattttcaca cacacacaca
     9841 ccactttcct tctggtcagt gggcacatgt ccagcctcaa gtttatatca ccacccccaa
     9901 tgcccaacac ttgtatggcc ttgggcgggt catccccccc cccaccccca gtatctgcaa
     9961 cctcaagcta gcttgggtgc gttggttgtg gataagtagc tagactccag caaccagtaa
    10021 cctctgccct ttctcctcca tgacaaccag gctcccaggt cccgaaaacc aaagaagaag
    10081 aacgcagatc gcagatctct agagtcaccg agttgcttta ccggatgaag agggccgaaa
    10141 catactgtcc aaggcccttg ctggcaatcc acccaactga agccagacac aaacagaaaa
    10201 ttgtggcacc ggtgaaacag actttgaatt ttgaccttct caagttggcg ggagacgtcg
    10261 agtccaaccc cggaccggtg catgtggacc ccaagaaaaa gcggaaggtg gaccctaaaa
    10321 agaaacgcaa agtggatcct aagaaaaaaa gaaaggtgca cgtgtccatg gtgtccaagg
    10381 gcgaggaact gttcaccggc gtggtgccca tcctggtgga actggatggc gacgtgaacg
    10441 gccacaagtt cagcgtgtcc ggcgagggcg aaggcgacgc cacatacgga aagctgaccc
    10501 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccttg gcctaccctc gtgaccacac
    10561 tgacctacgg cgtgcagtgc ttcagcagat accccgacca tatgaagcag cacgacttct
    10621 tcaagagcgc catgcccgag ggctacgtgc aggaaagaac catcttcttt aaggacgacg
    10681 gcaactacaa gaccagggcc gaagtgaagt tcgagggcga caccctcgtg aacagaatcg
    10741 agctgaaggg catcgacttc aaagaggacg gcaacatcct gggccacaag ctggagtaca
    10801 actacaacag ccacaacgtg tacatcatgg ccgacaagca gaaaaacggc atcaaagtga
    10861 acttcaagat ccggcacaac atcgaggacg gctccgtgca gctggccgac cactaccagc
    10921 agaacacccc tatcggcgac ggccctgtgc tgctgcctga caaccactac ctgagcaccc
    10981 agagcgccct gagcaaggac cccaacgaga agagggacca catggtgctg ctggaattcg
    11041 tgaccgccgc tggcatcacc ctgggcatgg acgagctgta caagcctgca ggcgagggca
    11101 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cgggccgtcc aatttactga
    11161 ccgtacacca aaatttgcct gcattaccgg tcgatgcaac gagtgatgag gttcgcaaga
    11221 acctgatgga catgttcagg gatcgccagg cgttttctga gcatacctgg aaaatgcttc
    11281 tgtccgtttg ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa tggtttcccg
    11341 cagaacctga agatgttcgc gattatcttc tatatcttca ggcgcgcggt ctggcagtaa
    11401 aaactatcca gcaacatttg ggccagctaa acatgcttca tcgtcggtct gggctgccac
    11461 gaccaagtga cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa gaaaacgttg
    11521 atgccggtga acgtgcaaaa caggctctag cgttcgaacg cactgatttc gaccaggttc
    11581 gttcactcat ggaaaatagc gaccgctgcc aggatataag gaatctggca tttctgggga
    11641 ttgcttataa caccctgtta cgtatagccg aaattgccag gatcagggtt aaagatatct
    11701 cacgtactga cggtgggaga atgttaatcc atattggcag aacgaaaacg ctggttagca
    11761 ccgcaggtgt agagaaggca cttagcctgg gggtaactaa actggtcgag cgatggattt
    11821 ccgtctctgg tgtagctgat gatccgaata actacctgtt ttgccgggtc agaaaaaatg
    11881 gtgttgccgc gccatctgcc accagccagc tatcaactcg cgccctggaa gggatttttg
    11941 aagcaactca tcgattgatt tacggcgcta aggatgactc tggtcagaga tacctggcct
    12001 ggtctggaca cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa
    12061 taccggagat catgcaagct ggtggctgga ccaatgtaaa tattgtcatg aactatatcc
    12121 gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc gatctcgagc
    12181 catctgctgg agacatgaga gctgccaacc tttggccaag cccgctcatg atcaaacgct
    12241 ctaagaagaa cagcctggcc ttgtccctga cggccgacca gatggtcagt gccttgttgg
    12301 atgctgagcc ccccatactc tattccgagt atgatcctac cagacccttc agtgaagctt
    12361 cgatgatggg cttactgacc aacctggcag acagggagct ggttcacatg atcaactggg
    12421 cgaagagggt gccaggcttt gtggatttga ccctccatga tcaggtccac cttctagaat
    12481 gtgcctggct agagatcctg atgattggtc tcgtctggcg ctccatggag cacccagtga
    12541 agctactgtt tgctcctaac ttgctcttgg acaggaacca gggaaaatgt gtagagggca
    12601 tggtggagat cttcgacatg ctgctggcta catcatctcg gttccgcatg atgaatctgc
    12661 agggagagga gtttgtgtgc ctcaaatcta ttattttgct taattctgga gtgtacacat
    12721 ttctgtccag caccctgaag tctctggaag agaaggacca tatccaccga gtcctggaca
    12781 agatcacaga cactttgatc cacctgatgg ccaaggcagg cctgaccctg cagcagcagc
    12841 accagcggct ggcccagctc ctcctcatcc tctcccacat caggcacatg agtaacaaag
    12901 gcatggagca tctgtacagc atgaagtgca agaacgtggt gcccctctat gacctgctgc
    12961 tggaggcggc ggacgcccac cgcctacatg cgcccactag ccgtggaggg gcatccgtgg
    13021 aggagacgga ccaaagccac ttggccactg cgggctctac ttcatcgcat tccttgcaaa
    13081 agtattacat cacgggggag gcagagggtt tccctgccac agcttgatga agatctgagc
    13141 tcttaattaa tgatcataat cagccatatc acatctgtag aggttttact tgctttaaaa
    13201 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
    13261 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    13321 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    13381 catgtctgga tccgtttaaa ctaactttaa ataattggca ttatttaaag ttacatatgg
    13441 cggccgccct aaggctcgac ctcgaaattc taccgggtag gggaggcgct tttcccaagg
    13501 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
    13561 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
    13621 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
    13681 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
    13741 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
    13801 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
    13861 gggctcaggg gcggggcggg cgcccgaagg tcttccggag gcccggcatt ctgcacgctt
    13921 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
    13981 atccatctag atctcgagca gctgaagctt accatgaccg agtacaagcc cacggtgcgc
    14041 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
    14101 taccccgcca cgcgccacac cgtcgatcca gatcgccaca tcgagcgggt caccgagctg
    14161 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
    14221 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
    14281 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
    14341 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
    14401 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
    14461 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
    14521 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
    14581 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
    14641 accgaaagga gcgcacgacc ccatgcatcg atgatatcag atccccggga tgcagaaatt
    14701 gatgatctat taaacaataa agatgtccac taaaatggaa gtttttcctg tcatactttg
    14761 ttaagaaggg tgagaacaga gtacctacat tttgaatgga aggattggag ctacgggggt
    14821 gggggtgggg tgggattaga taaatgcntg ctctttactg aaggctcttt actattgctt
    14881 tatgataatg tttcatagtt ggatatcata atttaaacaa gcaaaaccaa attaagggcc
    14941 agctcattcc tcccactcat gatctataga tctatagatc tctcgtggga tcattgtttt
    15001 tctcttgatt cccactttgt ggttctaagt actgtggttt ccaaatgtgt cagtttcata
    15061 gcctgaagaa cgagatcagc agcctctgtt ccacatacac ttcattctca gtattgtttt
    15121 gccaagttct aattccatca gaagctggtc gagtaacttt aaataattgg cattatttaa
    15181 agttaggcgc gccataactt cgtatagcat acattatacg aagttatgtc gagatattta
    15241 aatattagac ccagctttct tgtacaaagt ggttgatatc tctatagtcg cagtaggcgg
    15301 gcttcattga ggtagaagct gatgcagaag tcatggaagg attctgctaa ctggcttgct
    15361 cccagtggct tgctcagcct tctttcttat agaacccagg accaccagcc cagggatggt
    15421 accaccacga tgagctgggt gacaagtgca tttactcgcc catctagctc actgaccctt
    15481 attttagagt tttgttgcaa cggcagattt taaaatagtt tggtgcattc tgtaactaga
    15541 ttgtgaattg attcgtttgt ttttttgtgt aatgtgccct aaaatcatcg agtggctttt
    15601 atcttaagaa tcacttacag atactcactt tgttctaatt gattacttaa cagcactatt
    15661 ccaggcatac agaaaatttg gatccgaaca tggggctgct cacataataa ttcggatgga
    15721 gaatacatgg ctggacagct tgctgcctat ggctataaaa ttacaggtaa tgagatccat
    15781 aatgtatttt attgattata tttcagagtt gcagattttg gagacaggta gtctagcccc
    15841 agcagtttaa gggaatttta gagtaattta atgataggaa ggtagagcac taatagcgac
    15901 tatatattat tagataactg gttacatctt tcttgacttc tattgagatg gcgcaacgca
    15961 attaatgata acttcgtata gcatacatta tacgaagtta tggtctgagc tcgccatcag
    16021 ttcactgcag gagaagagac ctctctcctg tgtgggatct tggggcaggc ttgacgacag
    16081 gtgcctttgc ccactgagcc atctcacctg cctggttatt ttaaaataac cttaaaacct
    16141 tttgtatttt gaagtgaaac tgcaggaaat tcatgtaagg ccaaaggagg gcattagctg
    16201 tttggagtaa cagtcagtga gagctgcttc gtgggtatca ggacttaaac tggggtcctc
    16261 tggaagagca ccatggtttg aaatgttgag acatatatct agtcccactt ttagcaattt
    16321 ttattctaat tttttatgat actttatttt atgtatagtg gtacaattta tacactgaat
    16381 aacataagag gatgattata tgtatttttt tggtcagagt ttaactttgt agttctggct
    16441 ggtctggatc ttgttatgtc aactggttgg ccttgaactc acagcattga tccttctgcc
    16501 tctcttggga ttaagacata taccaccatg accagtctag cttcatattt actgtgcgta
    16561 tgtgcacttc cgtgggctct ggagatccgg actgttagct tgtggaggaa gtactttata
    16621 ccctgagacg tctccacagc cctccttgtt gtctttttta acactgtgga gttcgcagag
    16681 tctttacctc tgctaaacat atcctttgtt ttgagctgta tttccccaac tctctgtaga
    16741 cctcaagttg agacctgaaa cataatttgc atggtttctg gattactgat attaggtgtt
    16801 ttggttattg tggattagtg ggattgtgtt aatattatta atttaaattg tttttgttaa
    16861 ttaagtatgg gcctaaaata tcaatgaata taacataggt gtaaaattta gatttcatgt
    16921 actgaataca gggattattg gaaagaagtt aagatttctt aatagctact tgtagccatt
    16981 ttactatttg gtgaagaaac atgatttggg taaagatact tgaaattgct gtctgtaact
    17041 ccgagtctga tacatgataa ttctgcagga aaacgtaaga tttggagtga aggaaaagat
    17101 tcatcgttta aaaaaatatt gctctgagac agggattcag agtccccaag tagctgagga
    17161 tggcttgcgt ttctgctcct ccgtgtggtt tggtgctggg ccggaaccca gggcaggggt
    17221 gtgctaggca ggcaggcagg caggccacct tcggaggaga gcccatggag atgggtggtg
    17281 ttttcctagc aaacgtcaca cagaacaatt agtatcaaga agagtgggag atgatagaat
    17341 taaatgggca aggtggcaag taggtgaact ttcttcatgg gaactagtgt ttttaaaagg
    17401 aatatgttgt ggctgagctg gcaggtgcac ttggggaact gaggcaggag gactgtgagt
    17461 tgtaggtagc tgctgtgtag tgggacactg tctcagaagc aagtggagac gtgatgtgaa
    17521 cacctgcatc ccaggagcat cctccaggaa gcaggctgaa ggctgacctc tgccctagga
    17581 aggctctgtt gctgtttctg ttgaagagct tgccaaatta tctccagtgt cttcatttgt
    17641 gggaaactgt cgtgtcttct cacatgaagt gttataaaca agcgacctgt gggtaagatt
    17701 tggggcatgc tagtgaataa tgctgttcta gttaacagta gacatttcat cttttatttt
    17761 ttcttactct atagcccagg ctggccttaa actcacagga acactcctgc ctagtgagta
    17821 ctgggatttt tggtagttac atttctgttt gtcataattg gttctgggaa ctcttgaaga
    17881 gtagcagcaa ttcttgttta tttattgatt ttaacatact gcctcactgt ctctttcctc
    17941 tgtgaggacc aggctggcct cagctcatag agcagcagta cttcgtatgc tttctgggtc
    18001 tcttcagact ccctcataat acagttctat caaaaaactc attacaactg ggtacgtagt
    18061 tctagtaaaa tatgagcctg gcttccctag gtgggtcccg cattcacaac agttatgcta
    18121 atgtgtagtt atacctgttg ctcatgggta ttgcatgtgc atgatggttt gtgggaaagt
    18181 ccgcccccgg ttgagttctc ataggcacat gaggggactg tgtgcttgtg gaccatctta
    18241 tggaagtcag aactgagtct ttatcttggg tgtcctagtt gcagaaagcg taaacatctc
    18301 taggctgtta cattcttgat ggttcctttg tatctttgta tatagcttaa tcagttggca
    18361 agtatgttgt gagcatactg tgtattcgtg ggtagagttc taggaacttg tggagacaca
    18421 aaaggaagaa tctttatatt gcttaaatct tagaggttgt cttgggcaat gcttatttct
    18481 aatggattct tgctttctct tttaataata gagaaaagta ggatctgctg tagttaaaaa
    18541 cttttataat ctgtatttta gaaatactgt agcattttgt gtttgatttg aggaactctc
    18601 tcgtgtaggg agacagcaca gtggactgac tcaggtgtat agcgtactcc tgggctttgg
    18661 ccactcaatg cgaagtatct ttaacttgtg tgcctgaggt cagtttcatg tatttgattc
    18721 atgtatggag aattttatat aggaaatgca tacaaggatc acattactga ttaggttatt
    18781 aaaatgaaat ttttagtttg tgttgatgtt gacagaagta atgcttgtta cataaagtta
    18841 cttaaaaacc gcagtgacag caaatgttgc ctggttatct cagtttcctt tgctggcttg
    18901 tgcttttgtc acgtcacacg aacatatcaa gcttacctag atgataagtt acattaagac
    18961 acagagtaag ctgggtgtgg cgatacatgc ctttggtccc agcactccta agagggttat
    19021 aaatgatata cactctgatt gtgagtggtt taagttgtgg aagatgatca gtgttattga
    19081 ataccagtta tatccctcac cccctttttt atgcaaagta attttttctt gcttttaatt
    19141 gaaaagattt ttaaaaaacc aacaaaaccc atgttttctg attatatttc tcctccccca
    19201 gctcctccca gatttcccca cttccccact cgcccagggt ggaaaccctt tctctcatta
    19261 ggatacaaag aggcaataag taagtaaata aaataaaaaa tacaaacaaa ccagactagg
    19321 acagaacaaa caaacaagca aaccaaaagg aaaagtgtct gagataaagc tcaagaaaca
    19381 tcacacacgc aggaactcca taaaaataca aaatgggatg tcatttatac atgcatgcac
    19441 acatgcaaag gacctgtaag ggtaaaaaaa cagccctgaa aaaacattat gagaccgaga
    19501 acctccaaaa attctattga atttgtttta tgttggccat ctactgctgg atgtgaatga
    19561 ggcttagccc taagagtagt gactagactc cattggatca aatgttcttt tcctttctga
    19621 gcagttagca attggagata gcttctggac tagggtatgg gcatgtgtct acttcgcctc
    19681 tcactgctgg gaccccatct ggcttagacc tttacaggac ctataagtgc aatcacagtc
    19741 tctatgagtt catatgtgca tcttgctgtc ttttgaagac ctgcttcttt ggtgttctcc
    19801 accccctctg gctcttacaa tctttttgcc tcttctgcag ggttccctga gccctgaggg
    19861 gagggaatta atggagagac cccatgtagg gttgagtgtt ccaaggtctc ttactgtatg
    19921 cttattgtcc agttgtgagt ctctgtttat tctcatccac agctgatgct gactgaggaa
    19981 gatgctaatc tatgaatata gcagaatgtc attaagtatt gttttattgt tattttcctt
    20041 tagcagaaga gtagtatttg gatctcctat atccctggtc tagctattcc aagttcttgg
    20101 acacatgagc agaatcagag acaggttcat ctcatggagt gggactcaga tctaatcaga
    20161 cgttacttgg tttgtcctac gagtctcctg ccattattgc attagcacat tttgcatgta
    20221 gagcaccgtt acaacggtgc tcaaagaatt tgttgcttag ttggtgtcta cctttctcct
    20281 ttggtagcat gcagaatgtc ttctggttct gtgactgttt agtcagtagg agtcaaggct
    20341 ctaggtaggc aatagttgga ctcctccacg tgcagtgagt tgttgtcttc agcagtaggg
    20401 ctttactcta agcttgtgga gagcagtata tagttctgta gtcttggcac caatctgcat
    20461 tggccaacag cttgattgta cataactcca agaatacatt tttttttttt taattaaagg
    20521 catggggcca ccacacccgg ctttttcttt tttctttttt ctttttttta agttttattt
    20581 attagtatat gtaagtacac tgtagctgtc ttcagacaca ccagaagagg gcgtcagatc
    20641 tcattacggg tggttgtgag ccaacatgtg gttgctggga tttgaactca ggacctcggg
    20701 aatagcagtc agtgctctta accactgagc catttcacca gcccccagca agaatacatt
    20761 tttaaaagag aaaaattgta gtgaacagac ataatgtcca aataaaacta aagactttag
    20821 tgaaagtcat tagagtgtgc atatatgtgt ttgcatgtat gtgtgtgtta ataatgacta
    20881 tcatttggaa agatttttat tatataaagc catttatata atagggcatc acaatgttac
    20941 aatttgaaag gccattgata tatttaatta attgctggat gctatgcata attctctgtg
    21001 gcctcaaggg acaaatgtat ctttgagaaa tatagcagga
//