LOCUS       179058#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21886 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 179058
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000084086
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            9112..9198
                     /db_xref="ENSEMBL:ENSMUSE00000638380"
                     /rank="5"
                     /note="ENSMUSE00000638380"
     exon            7973..8053
                     /db_xref="ENSEMBL:ENSMUSE00000638381"
                     /rank="4"
                     /note="ENSMUSE00000638381"
     exon            7382..7471
                     /db_xref="ENSEMBL:ENSMUSE00000523639"
                     /rank="3"
                     /note="ENSMUSE00000523639"
     exon            6770..6856
                     /db_xref="ENSEMBL:ENSMUSE00000638384"
                     /rank="2"
                     /note="ENSMUSE00000638384"
     exon            6575..6661
                     /db_xref="ENSEMBL:ENSMUSE00000915448"
                     /rank="1"
                     /note="ENSMUSE00000915448"
     misc_feature    4275..9554
                     /note="5 arm"
     misc_feature    9582..9606
                     /note="B1 Gateway"
     misc_feature    9619..9666
                     /note="Frt"
     misc_feature    9667..10166
                     /note="mouse En2 intron"
     primer          complement(9735..9756)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9631..9654)
                     /note="FRT3"
     primer          13832..13850
                     /note="seq primer NF"
     misc_feature    13892..13899
                     /note="RE sites"
     misc_feature    15692..15725
                     /note="loxP"
     misc_feature    complement(13900..13931)
                     /note="Rox"
     misc_feature    complement(15652..15683)
                     /note="Rox"
     misc_feature    13932..13953
                     /note="polylinker"
     CDS             14512..15108
                     /note="puromycin N-acetyl transferase"
     misc_feature    14627..14632
                     /note="Mutated RsrII site"
     intron          10167..10541
                     /note="mouse En2 intron"
     misc_feature    10539..10541
                     /note="Splice Acceptor"
     exon            10542..10595
                     /note="mouse En2 exon"
     primer          10442..10463
                     /note="seq primer FCHK2"
     misc_feature    10596..10718
                     /note="1Dleader"
     misc_feature    10719..10770
                     /note="F2A"
     misc_feature    10866..11582
                     /note="EGFP"
     misc_feature    10776..10865
                     /note="3X NLS"
     misc_feature    13648..13886
                     /note="SV40 polyadenylation site"
     misc_feature    12681..13625
                     /note="ERT2"
     promoter        13964..14475
                     /note="PGK promoter"
     misc_feature    15219..15651
                     /note="PGK pA"
     primer          13706..13729
                     /note="LRPCR RAF5"
     misc_feature    11592..11645
                     /note="T2A"
     misc_feature    11642..11642
                     /note="A to G to kill RsRII site"
     misc_feature    11646..12671
                     /note="Cre"
     misc_feature    10557..10596
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    10557..10572
                     /note="FCHK QC prefix region"
     misc_feature    10573..10590
                     /note="FCHK QC critical region"
     misc_feature    10591..10596
                     /note="FCHK QC suffix region"
     misc_feature    15748..15768
                     /note="B2 Gateway"
     misc_feature    9555..15798
                     /note="Synthetic Cassette"
     exon            18043..18194
                     /db_xref="ENSEMBL:ENSMUSE00000638376"
                     /type="targeted"
                     /rank="8"
                     /note="target exon 1 ENSMUSE00000638376"
     exon            17465..17551
                     /db_xref="ENSEMBL:ENSMUSE00000638378"
                     /type="targeted"
                     /rank="7"
                     /note="target exon 2 ENSMUSE00000638378"
     exon            16153..16239
                     /db_xref="ENSEMBL:ENSMUSE00000638379"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 3 ENSMUSE00000638379"
     misc_feature    15799..18366
                     /note="Critical Region"
     misc_feature    18390..18423
                     /note="Downstream LoxP"
     misc_feature    18367..18446
                     /note="synthetic loxP region"
     exon            20730..21886
                     /db_xref="ENSEMBL:ENSMUSE00001043174"
                     /rank="10"
                     /note="ENSMUSE00001043174 5' fragment"
     exon            19498..19607
                     /db_xref="ENSEMBL:ENSMUSE00001318244"
                     /rank="9"
                     /note="ENSMUSE00001318244"
     misc_feature    18447..21886
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaccctga tataactgtc ctgcgatgct attccagggc ctagcaaact
     4321 cataagtgga tgctcacagt tagctattgg atggatcaca gggcccccaa tggaggagct
     4381 agagaaagta cccaatgagc taaagggatc tgcaacccta taggtggaac aacaatatga
     4441 actaaccagt atcccccgga gatcgtgtct ctagctgcat atgtatcaga agatgaccta
     4501 gtgaaaagag aggccatcag tgaaaagaga ggccatttgg tcttgcaaac tttatatgcc
     4561 tcagtacagg ggagcgttat ggccaagaag tggtagtggg ggggatggga ttggggggat
     4621 gttgggggac ttatggggta gcattggaaa tgtaaatgaa gaaaatatct aattaataaa
     4681 aaaaagaaag aaaatgaata tgtggcttga tatcttatat aaacagcagc ctctagcatc
     4741 tcaggaagta tctgtcctta gggaaggtta gaggagaaac ttttcatttt ctccttggaa
     4801 ctatctgatt atctttggtt tacaacatag aagcagatta cccacattca attctcagct
     4861 tcactgtgaa ccagctgtct gattattatt agacagttgc ctattctgac tttgcagttt
     4921 cccagtatta cagtatttgt agttttttgt attacagatc tcttaggcat ctcttctttt
     4981 aattaaaacg taaaaacata actttggctc ttacatacgt tgctgtctta tcctgattcc
     5041 attttgtatc tgcctacttt ccagccttct acctgcaaca gccatgccta ctttggtaac
     5101 acatttggga ttcccataac cctaattatg gtttacaaca tagaagcaga atagaatatg
     5161 cattagccaa agcaattaat gtttatacaa actaaacatg aactaaaata acatttcccc
     5221 actccaggtt acactttgaa cattttaaac agagacagga gactataggt ctgcacttag
     5281 accaatagga gatatacaaa acaaagcaaa aaatatttta agaatatcaa tgttattttt
     5341 caagcagtca ttttctggga ttatcttgct cccctagcag aattatgttt gtgtcaagaa
     5401 gaaagtgagg ggcttcctgg caggtcagga cttaacactg gttgtcacct tgtgcttcat
     5461 tcttgtgatg ccatagaggc attgtcatag aagatgggcc taatggctat tgtagatctc
     5521 tgttgtctcc tttgtcctgg gaatggagtc aaatagccat tatatccagg taaataaaat
     5581 ctttatatcc tataatcatg aatttagctt atttccaaca ggcctggtta tcaggtcact
     5641 catcttatgt tttgagaaag ggtctctcaa tgaacctgga actatcgatt tgactaggtt
     5701 agtagatcaa tgaactcctg tgaccatcct gtccacttct tacaacactg gagttacagg
     5761 tgagtgctgc cacgtctggc tttttacctg ggtgctggga tctgaactca tgcttgtgca
     5821 gtaagcactt tacccaatga accaccttgc caacttggct tgttttccca aaattctaca
     5881 attctgatat agctagggaa cttttcattt tctccttgga actatctgat tatctttggt
     5941 ttacaacata gacgcagatt acccacattc aattctcagc ttcactgtga accagctgtc
     6001 tgaatattat tagacagttg cctaatctga ctttgcagtt tcccagctac cacagtgcaa
     6061 tgaggtttga ggaatgtttc cacccatcag tcagaaatcc tgaccacagg ttctcaatag
     6121 agaggtgcat gagaacagca aagtcagcag aaatacccac tgttcaggac atagcaaaga
     6181 aagatagcct ggagtcagaa gggcagagga actgggaaga tgggatattg ctctcctaca
     6241 ttcagcatag gtgaaaatat cgctctccta cattcagcat aggtgaaaag ggcataacaa
     6301 agtagattta ttgtattggc tttgaaatgg cacccataat ttggtcacac tatgaacaca
     6361 ccagttcttc ctgctacaaa attccaaaat tcaataccct tccttaaaaa acaaacaaac
     6421 aaacaaacaa aaaactaagt cttgatatat ctggcaaaag ataggctgtg tcaaaggtgc
     6481 agcccctccc ctaggctctt ccgcaataca tgggcttcct ttttcccctt ccggagtgaa
     6541 aggacattta gagaacttca ggggataaaa gctgtagtct ccagcattcc catgcaccag
     6601 gggacagcgg caaccatgag tgactccaca gaagccaaga tgcagcctct tagctccatg
     6661 ggtgaggctg ggctgggttc tgggagcatg gtgagggaag tcagagggtt ctgctcagag
     6721 gtgagcccgc ccagctgact ggtgcttgac tggtgctctc ttccctcaga cgatgatgag
     6781 ttgatggtca gcggcagcag gtattctatt aaaagctcca gactacgacc aaattctgga
     6841 atcaagtgtt tggcaggtac aacatggtgg ggggctgaga aaaaaatggg tcttttcctc
     6901 aaagtgggtt ttcgggagtt gaaggctagg actctgaact tcctcttctt gggaagatag
     6961 aggcgggaca aggaatggag cgctacggga aggctggggt agatggctag gggagacatc
     7021 acaggttgtg gaaggaggag gctagggaag gaaatcatgg tccatgggaa aggggatggt
     7081 aggacacgaa aacttgggct atgatactgt ggaagttggg acagggaatt gagggtcctg
     7141 gaaaatggag ggtgatgcaa ggaattaaga gtcttgggga agtagaagct ggggtaggga
     7201 actgagggtg tgggtagatg gaggatggag cagaaaatca gaagctttga gaagattgag
     7261 gccagggaac aaaaccatgg cccatgggga ggtagaatct gagtctagat tcacatgcca
     7321 ggtgggaggg ggacgctgtt ctggaaccag gctactaaaa agtccacttt ttccctggaa
     7381 ggatgctcgg gacacagcca agtccccttg gtcctgcagc tgctctcctt cctgttcttg
     7441 gctgggctcc tgctgatcat tcttttccaa ggtcaggcac aatgaagggc ttagctcttg
     7501 caggaccttc acctagggcc attttaggtc tgggagggaa atggacagtg caggtggatg
     7561 gcatatcttt aaaggacgtc cctgactctg gtataggaca gaatgagaaa aagaagcatg
     7621 ggtggatcct ggattcattt ctccttatgg ctcagcatct tctgagtggc aggttttcta
     7681 atcttcaaac agaattaagg aagatattaa tgagcaactg cctcagactc tggagtactg
     7741 ccatgtgcct atagtcccag ctactgggga agctgaggca caatgataat ttgtgccaaa
     7801 gaatatgaga gaagccttga caacataatg agacctatct ttaaaaaaaa aaagaaagaa
     7861 agaaagaaag aaagaaagaa agaaagaaag aaaagttgac agtgtatctg agtgcaggac
     7921 ttgacacaca ggaagtattt aataatgaaa ttatttttcc ccttcctcac agtctccaaa
     7981 accccaaata ccgagaggca gaaggaacaa gagaagatcc tccaggaact gacccagctg
     8041 acagatgagc ttagtgagtg accaatactc ggaagtatca tggactcagg aaggctctag
     8101 ggaaaggggt caattttcct aaattgtcag atctcttaga gcctcaattt tctcaagtct
     8161 gtgaattcat accacaagat taacaacaac aaaaaatggt acttggtatc aatatgagtt
     8221 gttacacagt aggttctgag tgtcttcagg ggctatgttc atagtaagta ctcaatatcc
     8281 acaaggatca tatacacagt agacattcaa tgttcccaga agacatgcac atacttggca
     8341 cacaacatgt actgtgcatg cagcagatac tacatattca ttggggccat aaacacatgt
     8401 aatattcaca ggcctcacat acacagtcag tactcaattg aatagttctc ttgggttttg
     8461 ttctgattgc taggcatcaa attctcccag tcaagaggat aatactgggg actccagcag
     8521 gttcaggttg tgggtgggag gtgttgggaa gtgaataagg gaatgtttcc ctaggatgct
     8581 gtgtcactct ggagggcctg aggttaagta gacaacaaac ataatcatgt aagggtgggt
     8641 ttatcctgac ctgcctctcc tgggattaga tgctcttctc agtgttctgt ttcccagtct
     8701 tcaaaggaga taaaacaaaa catcaatcag aaactgtcct agatgctgga atggcagcac
     8761 ccacctgtag tctgagctac tggggaaggt aagggaggga taaccactta tgtgcaggag
     8821 tttgatagaa gcatagatgc caactgaaat ttatctaaaa agtaaaataa cacatgagcc
     8881 ggtgagatag cttagcagac aaaagtgctt gcttccaagc ctaatgacct gaattcaatc
     8941 ccttgaactc acctggcagg aggagaactc ctacaagtct tcctctgacc tccattcaca
     9001 caaacatgta tgccccctgg tcccacatac aataaataaa tgcttttttt tttggagacc
     9061 agtgatacat ggtagatatt taataatggc actttgtttt tcttgacaca gcgtccagga
     9121 tccccatctc ccaagggaag aatgagtcca tgcaggcgaa gatcactgag caactgatgc
     9181 agctgaaaac tgaactctgt gagtgagaaa gcagcagtcc aagctgctgg gtctgtaggg
     9241 gccagttttg agccatcaga cctctttgag ccttggtttc ctcatctgta aattgaaaac
     9301 acaaagtaac agcaaggatg atatttgctc cttacaggga ctgtacatgc aaaagaactc
     9361 acatccccag gggttgttca cagtgtaggc actcagtgct ccaaaggacc atgcatacat
     9421 accttcaacg tctaatgttc acagtggtca tatgtacaga gtaaactcaa tgttcacagt
     9481 gctgtacaca gagtagatat tcaattcaat gtcttgactc ctattctgaa ctctctctct
     9541 atttcaccta tcagaaggcg cataacgata ccacgatatc aacaagtttg tacaaaaaag
     9601 caggctggcg ccggaaccga agttcctatt ccgaagttcc tattctctag aaagtatagg
     9661 aacttcgaac cctttcccac accaccctcc acacttgccc caaacactgc caactatgta
     9721 ggaggaaggg gttgggacta acagaagaac ccgttgtggg gaagctgttg ggagggtcac
     9781 tttatgttct tgcccaaggt cagttgggtg gcctgcttct gatgaggtgg tcccaaggtc
     9841 tggggtagaa ggtgagaggg acaggccacc aaggtcagcc ccccccccct atcccatagg
     9901 agccaggtcc ctctcctgga caggaagact gaaggggaga tgccagagac tcagtgaagc
     9961 ctggggtacc ctattggagt ccttcaagga aacaaacttg gcctcaccag gcctcagcct
    10021 tggctcctcc tgggaactct actgcccttg ggatcccctt gtagttgtgg gttacatagg
    10081 aagggggacg ggattcccct tgactggcta gcctactctt ttcttcagtc ttctccatct
    10141 cctctcacct gtctctcgac cctttcccta ggatagactt ggaaaaagat aaggggagaa
    10201 aacaaatgca aacgaggcca gaaagatttt ggctgggcat tccttccgct agcttttatt
    10261 gggatcccct agtttgtgat aggcctttta gctacatctg ccaatccatc tcattttcac
    10321 acacacacac accactttcc ttctggtcag tgggcacatg tccagcctca agtttatatc
    10381 accaccccca atgcccaaca cttgtatggc cttgggcggg tcatcccccc ccccaccccc
    10441 agtatctgca acctcaagct agcttgggtg cgttggttgt ggataagtag ctagactcca
    10501 gcaaccagta acctctgccc tttctcctcc atgacaacca ggctcccagg tcccgaaaac
    10561 caaagaagaa gaacgcagat cgcagatcgc agatctctag agtcaccgag ttgctttacc
    10621 ggatgaagag ggccgaaaca tactgtccaa ggcccttgct ggcaatccac ccaactgaag
    10681 ccagacacaa acagaaaatt gtggcaccgg tgaaacagac tttgaatttt gaccttctca
    10741 agttggcggg agacgtcgag tccaaccccg gaccggtgca tgtggacccc aagaaaaagc
    10801 ggaaggtgga ccctaaaaag aaacgcaaag tggatcctaa gaaaaaaaga aaggtgcacg
    10861 tgtccatggt gtccaagggc gaggaactgt tcaccggcgt ggtgcccatc ctggtggaac
    10921 tggatggcga cgtgaacggc cacaagttca gcgtgtccgg cgagggcgaa ggcgacgcca
    10981 catacggaaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccttggc
    11041 ctaccctcgt gaccacactg acctacggcg tgcagtgctt cagcagatac cccgaccata
    11101 tgaagcagca cgacttcttc aagagcgcca tgcccgaggg ctacgtgcag gaaagaacca
    11161 tcttctttaa ggacgacggc aactacaaga ccagggccga agtgaagttc gagggcgaca
    11221 ccctcgtgaa cagaatcgag ctgaagggca tcgacttcaa agaggacggc aacatcctgg
    11281 gccacaagct ggagtacaac tacaacagcc acaacgtgta catcatggcc gacaagcaga
    11341 aaaacggcat caaagtgaac ttcaagatcc ggcacaacat cgaggacggc tccgtgcagc
    11401 tggccgacca ctaccagcag aacaccccta tcggcgacgg ccctgtgctg ctgcctgaca
    11461 accactacct gagcacccag agcgccctga gcaaggaccc caacgagaag agggaccaca
    11521 tggtgctgct ggaattcgtg accgccgctg gcatcaccct gggcatggac gagctgtaca
    11581 agcctgcagg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg
    11641 ggccgtccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc gatgcaacga
    11701 gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg ttttctgagc
    11761 atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc aagttgaata
    11821 accggaaatg gtttcccgca gaacctgaag atgttcgcga ttatcttcta tatcttcagg
    11881 cgcgcggtct ggcagtaaaa actatccagc aacatttggg ccagctaaac atgcttcatc
    11941 gtcggtctgg gctgccacga ccaagtgaca gcaatgctgt ttcactggtt atgcggcgga
    12001 tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca
    12061 ctgatttcga ccaggttcgt tcactcatgg aaaatagcga ccgctgccag gatataagga
    12121 atctggcatt tctggggatt gcttataaca ccctgttacg tatagccgaa attgccagga
    12181 tcagggttaa agatatctca cgtactgacg gtgggagaat gttaatccat attggcagaa
    12241 cgaaaacgct ggttagcacc gcaggtgtag agaaggcact tagcctgggg gtaactaaac
    12301 tggtcgagcg atggatttcc gtctctggtg tagctgatga tccgaataac tacctgtttt
    12361 gccgggtcag aaaaaatggt gttgccgcgc catctgccac cagccagcta tcaactcgcg
    12421 ccctggaagg gatttttgaa gcaactcatc gattgattta cggcgctaag gatgactctg
    12481 gtcagagata cctggcctgg tctggacaca gtgcccgtgt cggagccgcg cgagatatgg
    12541 cccgcgctgg agtttcaata ccggagatca tgcaagctgg tggctggacc aatgtaaata
    12601 ttgtcatgaa ctatatccgt aacctggata gtgaaacagg ggcaatggtg cgcctgctgg
    12661 aagatggcga tctcgagcca tctgctggag acatgagagc tgccaacctt tggccaagcc
    12721 cgctcatgat caaacgctct aagaagaaca gcctggcctt gtccctgacg gccgaccaga
    12781 tggtcagtgc cttgttggat gctgagcccc ccatactcta ttccgagtat gatcctacca
    12841 gacccttcag tgaagcttcg atgatgggct tactgaccaa cctggcagac agggagctgg
    12901 ttcacatgat caactgggcg aagagggtgc caggctttgt ggatttgacc ctccatgatc
    12961 aggtccacct tctagaatgt gcctggctag agatcctgat gattggtctc gtctggcgct
    13021 ccatggagca cccagtgaag ctactgtttg ctcctaactt gctcttggac aggaaccagg
    13081 gaaaatgtgt agagggcatg gtggagatct tcgacatgct gctggctaca tcatctcggt
    13141 tccgcatgat gaatctgcag ggagaggagt ttgtgtgcct caaatctatt attttgctta
    13201 attctggagt gtacacattt ctgtccagca ccctgaagtc tctggaagag aaggaccata
    13261 tccaccgagt cctggacaag atcacagaca ctttgatcca cctgatggcc aaggcaggcc
    13321 tgaccctgca gcagcagcac cagcggctgg cccagctcct cctcatcctc tcccacatca
    13381 ggcacatgag taacaaaggc atggagcatc tgtacagcat gaagtgcaag aacgtggtgc
    13441 ccctctatga cctgctgctg gaggcggcgg acgcccaccg cctacatgcg cccactagcc
    13501 gtggaggggc atccgtggag gagacggacc aaagccactt ggccactgcg ggctctactt
    13561 catcgcattc cttgcaaaag tattacatca cgggggaggc agagggtttc cctgccacag
    13621 cttgatgaag atctgagctc ttaattaatg atcataatca gccatatcac atctgtagag
    13681 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
    13741 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    13801 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    13861 ctcatcaatg tatcttatca tgtctggatc cgtttaaact aactttaaat aattggcatt
    13921 atttaaagtt acatatggcg gccgccctaa ggctcgacct cgaaattcta ccgggtaggg
    13981 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
    14041 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
    14101 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
    14161 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
    14221 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
    14281 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
    14341 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ttccggaggc
    14401 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
    14461 ccgggccttt cgacctgcat ccatctagat ctcgagcagc tgaagcttac catgaccgag
    14521 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
    14581 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccaga tcgccacatc
    14641 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
    14701 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
    14761 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
    14821 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
    14881 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
    14941 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
    15001 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
    15061 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc
    15121 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcgat gatatcagat
    15181 ccccgggatg cagaaattga tgatctatta aacaataaag atgtccacta aaatggaagt
    15241 ttttcctgtc atactttgtt aagaagggtg agaacagagt acctacattt tgaatggaag
    15301 gattggagct acgggggtgg gggtggggtg ggattagata aatgcntgct ctttactgaa
    15361 ggctctttac tattgcttta tgataatgtt tcatagttgg atatcataat ttaaacaagc
    15421 aaaaccaaat taagggccag ctcattcctc ccactcatga tctatagatc tatagatctc
    15481 tcgtgggatc attgtttttc tcttgattcc cactttgtgg ttctaagtac tgtggtttcc
    15541 aaatgtgtca gtttcatagc ctgaagaacg agatcagcag cctctgttcc acatacactt
    15601 cattctcagt attgttttgc caagttctaa ttccatcaga agctggtcga gtaactttaa
    15661 ataattggca ttatttaaag ttaggcgcgc cataacttcg tatagcatac attatacgaa
    15721 gttatgtcga gatatttaaa tattagaccc agctttcttg tacaaagtgg ttgatatctc
    15781 tatagtcgca gtaggcggag atttgaagag aaaaggaaca taggtattca ttcaagtgtg
    15841 ggctcattct aacccccaac cccagctcca ggattagatc ccttctgaat gttgactctc
    15901 ccagtctcca ggaaagtaaa catggcatca atcagaaacc atcccataaa ctggggtggc
    15961 aacctctgcc tgcagtccta gccactgggg aactggaaag gggatgttta tttgtgcctg
    16021 aatttgtaac aagctgtgtc atagtgtggt tcaaaaaata tgacatgaga tctcccaggg
    16081 ataggcaatg gcacacagta gctatttaat aatggcagtt ctttactttt ttctccattt
    16141 ttcttggcac agtgtccagg attcccatct tccaggggca gaatgagtcc atacaagaga
    16201 agatctctga gcaactgatg cagctgaagg ctgaacttcg tgagtgataa aaaaccagag
    16261 gttctgaggc ctccagacac tataggggtc aattttcttg agtcatttga cttatttatg
    16321 cctcagtttc ttcatctgtg aattgaggcc acaagtggca gcaaggacaa cacttgatgt
    16381 ttacagaggc acccagtagg cattcagtgt tgatagggac catacacaca ttagatactt
    16441 ggtgtttata agcaccatgc atacattagg tactcagtat tcacagggcc atgcacacag
    16501 taggtactat gtgttcatag ggtccatata tacactaggc actcaacatt tacaaagcct
    16561 gtattggtta ggttctctga gagaacaaat tctatagaat gaatatttat caaaaggctc
    16621 tactagatta gtttacacaa tatggtctaa caagtccaca gtagctgtct acatgctgca
    16681 gaggatgaga acatagtaga tgcccagtcc ttgagtctgg atgccacaga gtcctgatct
    16741 ggggcctgga ggattcctgg aaagcacaga tcatcaggcc acaatgaaaa tctgaaggtg
    16801 taaggatcaa catcagcaga gaatagatgc accagagaca gcaatagggt agatgggaag
    16861 atggacagac aacagcagcc ctgccatcac tggcaggtgt tccttaccct gttaatcttt
    16921 cctagaaata ccctcatagc cctgccctaa ggtgtgtctc ttggttgtca ttctagatac
    16981 aatcatatta acatcaattg tgctgtgaac acagtaggga ctcaattttc actgaatttg
    17041 tacacatagt aggcagtcag tgatcacagg gattgtgcac agagtcatca cacagtgttc
    17101 ttgaagttat gcacagagta ggaactcaat attcagaggt aacatgcaca tgtttggctc
    17161 tcagttaagt cagtctactg gcctctgttc tcaccacaca ggagaggaag tggaggcaag
    17221 attcctgggt gttaggtctc tgaatcttta taggaaaaaa ctgtccctga tcctgggttg
    17281 tggtacttgc ctgaaatcca agctcctcag gaggccaagg gatgttgtat ccttttgcct
    17341 aggagtttga gatgttccga caacagggtg agctccatct caaaagagaa atgacatgtc
    17401 cctggggatg tagttcaagt cagtgtactc agctgcagtt ccccatcttt ttttcttggt
    17461 ccagtttcca agatctccag cttcccggta aaggatgatt ctaagcagga gaagatctac
    17521 caacagctgg tacagatgaa gactgaactc tgtgagtgaa cagaaaccag aggcctcagg
    17581 agcctcaggc agccattact ttgggtcaaa ttctttgagt tttcagacat ctttgatctt
    17641 cagttccctc tctaaattgg gcctataatg tagcagcaag gatagcaatt ggtatttata
    17701 ggagttctgc aaacagcagg cactcaatat taacaggggc tgtgcacaat gaacactgag
    17761 tgtgcatagt tgtgcacact tcaggaactc agtgttcaca aacaccataa gctcatcttc
    17821 tatcctctgt tctgaacttt aacccctact ccttccaaac taaagaaaga agagctagga
    17881 ctcaggagtc ttcctgagtt cccctagagt ttcaaaggga tgtagaaagc agaaaagaaa
    17941 tggttcccca ggatgctgtg tcaagggagg gccctgactc aggagagttt gaaggctaat
    18001 caggcacaag atcacaggca tcctaaccca cttccccaac agtccgcctg tgtcgactct
    18061 gcccctggga ctggacattc ctcctaggaa attgttactt cttctccaag tcccagcgga
    18121 actggaatga cgccgtcaca gcttgcaaag aagtgaaggc tcaactagtc atcatcaata
    18181 gtgatgaaga gcaggtacat ttagtgggac tccatccttg tctggtctgt gcacctggct
    18241 cctgatcaca taagcttaag ataatttgcc agagttatgt gaggaaaaga agagacgggg
    18301 gtcctgagcc ctttagatag aacatttgtg agtgctttgg ggacacctaa aatgcagtag
    18361 atatcagaga tggcgcaacg caattaatga taacttcgta tagcatacat tatacgaagt
    18421 tatggtctga gctcgccatc agttcagcct gctaattaaa tagcacagca gacaaagtgc
    18481 ttgcctctaa gcctcacacc ctgagttcaa ttcctaggac ccccgtggta ataggagaga
    18541 atcaactctt taagatgccc tctgacagac atacctgcac tgtggcatat gcccatccag
    18601 acatagaagt agacataatt ataagaaaaa atgaagtcct gacatgggtc tgcttcacaa
    18661 gcttgaagac cttgagttca aatccaaaga taaagaggaa tggggtgaga tggctcagta
    18721 gatgaaggcg cttgctgcca agcctgacaa tttatcctct gaacctacat ggttcagaga
    18781 accaacccct ataaattgtc ctttgacctc tatgtacagg catattaata tccataagac
    18841 atatacatag ataagcatgc atacacgcat aacctgatat gatatgtaat tctcctggtg
    18901 taaatattct caccatgaac agcttcaagt ttataatggg atgccactga agaactgaaa
    18961 agaaagggta tgtttcttag gtaaccatga attagcatca tttgcttcta tcacattaaa
    19021 gccagccatg aggaaattct atagactcat tctatagact cagaattctt aagagccatc
    19081 cagcaacatt agcagaattg gggagggatg aacagctgtg taattcgtgt gtgtgcgtgt
    19141 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgttgctat ctttcagaca
    19201 gggtctcact ctagttctat ctggcctgga actcacagaa atcctgtggg tttctgagat
    19261 taaaggcatg taccgtcatg tccatgcagc taggtaccac cctatgccaa gtgaatgtac
    19321 tgccataggc tacagatgta aaaatagctg acaccatcta agcacacatg agtactcatg
    19381 gcctgatata gcataagggg aaaagataat gttgtaagta gaagtttctg cctgaggttg
    19441 gggagacaag ctgaaagtag gaaactctcc cctgactgtc ctcctcttta cctccagacc
    19501 ttcctgcagc agacttctaa ggctaaagga ccaacctgga tgggcctgtc agacctgaag
    19561 aaggaggcca cgtggctctg ggtagatggt tctactctgt catccaggta gatccagtga
    19621 acaaccagta gtgtagtcca gacaggtctc tgatgttctg atagctacct gtgagtctca
    19681 tcctcttctt cctcacctct tagaaataag agccacccat attagctgat ggaaggaagt
    19741 tttcctaccc atgcacagat agaatatcag caaatcccac catcaaagca acaagaaaaa
    19801 cttccccctg aggggtgtta attttaatgt aggctattag tagaaaatac aaaataaagt
    19861 aaggccacag ggtaagggga ctattaacat tgaaagatat attattgggt tggggatata
    19921 aatatgttca ttaagtagca taaggtttga tccccccatt gcatagtctg atatggtggt
    19981 ccatgcctat aacaccagca gttggaaggt agagggagaa gaatcagttc aatgttatct
    20041 tcagctacaa aatgggttct gagacagcct gagctacaca ggaaggaagg gggagagaga
    20101 aagagaaaag caggtaggga aggaaatgta aagaagggga agaggacaac agtagcttaa
    20161 agggagttta tatggatgtt caccgatccc acagcacaac acagagaaca tggggaagaa
    20221 ccacaacaaa tcagaataaa aaggaaggac acactgtggg aaagaacctt tactttgttg
    20281 tccatgcata agaacaggag agactggctt ggcatctatt agtttaccat tttaactttc
    20341 tgatccctaa ttgtcttaca catgcttggc atgtggcaga tgtattgtgg accagaatgc
    20401 aagaacccag taaggaaggc aaaatccaga ttgattggtt tacaccacaa aaataaaata
    20461 aaataaaata aaataaaata aaataaaata aaataaaata aaataaaata aaatagaaaa
    20521 acccacacca ctccttcaat gtaggatata acaagagtca ttttccaaag ccagcagacc
    20581 caggcatgcc cccatctcca gaggaaggcc catctacaat atgcattctg gaaggaggta
    20641 aagacattga gtttcagaaa gctaaaaata tggcatggtt cacagcatgc cactggagca
    20701 gactaacgtt tttctggctg actttccaga ttccagaaat attggaatag aggggagcct
    20761 aacaacatcg gtgaggaaga ctgtgtcgaa tttgctgggg atggctggaa tgactctaaa
    20821 tgtgaactca aaaagttctg gatctgcaag aagtctgcaa ccccatgcac tgaaggctag
    20881 ctcatctccg ctcctacctt catgccattc tgccaggcac atggatgtgc ctcactttcg
    20941 tgccagctcc ttcttcctgc ctgttggcct caggatcgtg aaaaaggctc tgggattctt
    21001 ctttttatca gatttttcat cctctgcatt tatcatagtt tcatttctgt tgatgtgata
    21061 aaactctcta accaaaaaca actaaggcag aaaggggttt attttacctt acattccaga
    21121 tgagagcagg gaggtcagga tggcaggaac ttaagacaat tggtcacatc acatcaacag
    21181 ccaggaggag acagaagtat gtgcaagcta acgcacacat ttgcttattc tcagttccat
    21241 tttcctattc ctatagttta ggacccctgc ctaggaaatg gtgccattca tagttggcta
    21301 gatgttccca cctctgttaa cttcaataag acaagccccc acagacatgc tcactggcca
    21361 acccaatgca ggcaatacac cattgagcct cttcccaggt gactatacgc tgtgtcaggc
    21421 tgactatcac aaagggactc actattgtcc ttttgctttc aggtttctct gggcttaggg
    21481 ctcactgttg tccccccact tcagtgcagt tacctgatgt aggatgcttt cagaatctgg
    21541 gtgttttcca ttaacctact ccatcttcct tgctgagaca catacactca aaggtcagaa
    21601 gaagacattg gtgttctctt tgatagctct ttactccctt gagacaggag ttggagttca
    21661 ttattttttt agcaaggctg gtgatctgaa agatccaaca tttttctgtc tccactcacc
    21721 tcctaaatga agttacagtc acatgtggcc atgtctggcc tctaacaatt actagggctt
    21781 caaggtcagg ccctctttct tgcacatcag cactcttatc cactaagcat ctccccagcc
    21841 atttgtctat tgtcaggtag tctagtttac tgttgctgta ataaag
//