LOCUS       82395#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20160 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 82395
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000050776
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            5338..6536
                     /db_xref="ENSEMBL:ENSMUSE00000381057"
                     /rank="1"
                     /note="ENSMUSE00000381057"
     misc_feature    4275..9734
                     /note="5 arm"
     misc_feature    9762..9786
                     /note="B1 Gateway"
     misc_feature    9799..9846
                     /note="Frt"
     misc_feature    9847..10346
                     /note="mouse En2 intron"
     primer          complement(9915..9936)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9811..9834)
                     /note="FRT3"
     primer          14012..14030
                     /note="seq primer NF"
     misc_feature    14072..14079
                     /note="RE sites"
     misc_feature    15872..15905
                     /note="loxP"
     misc_feature    complement(14080..14111)
                     /note="Rox"
     misc_feature    complement(15832..15863)
                     /note="Rox"
     misc_feature    14112..14133
                     /note="polylinker"
     CDS             14692..15288
                     /note="puromycin N-acetyl transferase"
     misc_feature    14807..14812
                     /note="Mutated RsrII site"
     intron          10347..10721
                     /note="mouse En2 intron"
     misc_feature    10719..10721
                     /note="Splice Acceptor"
     exon            10722..10775
                     /note="mouse En2 exon"
     primer          10622..10643
                     /note="seq primer FCHK2"
     misc_feature    10776..10898
                     /note="1Dleader"
     misc_feature    10899..10950
                     /note="F2A"
     misc_feature    11046..11762
                     /note="EGFP"
     misc_feature    10956..11045
                     /note="3X NLS"
     misc_feature    13828..14066
                     /note="SV40 polyadenylation site"
     misc_feature    12861..13805
                     /note="ERT2"
     promoter        14144..14655
                     /note="PGK promoter"
     misc_feature    15399..15831
                     /note="PGK pA"
     primer          13886..13909
                     /note="LRPCR RAF5"
     misc_feature    11772..11825
                     /note="T2A"
     misc_feature    11822..11822
                     /note="A to G to kill RsRII site"
     misc_feature    11826..12851
                     /note="Cre"
     misc_feature    10737..10776
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    10737..10752
                     /note="FCHK QC prefix region"
     misc_feature    10753..10770
                     /note="FCHK QC critical region"
     misc_feature    10771..10776
                     /note="FCHK QC suffix region"
     misc_feature    15928..15948
                     /note="B2 Gateway"
     misc_feature    9735..15978
                     /note="Synthetic Cassette"
     exon            16364..16586
                     /db_xref="ENSEMBL:ENSMUSE00001263224"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00001263224"
     misc_feature    15979..16746
                     /note="Critical Region"
     misc_feature    16770..16803
                     /note="Downstream LoxP"
     misc_feature    16747..16826
                     /note="synthetic loxP region"
     exon            17960..20160
                     /db_xref="ENSEMBL:ENSMUSE00000831237"
                     /rank="3"
                     /note="ENSMUSE00000831237 5' fragment"
     misc_feature    16827..20160
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaacatcc tcgtgctagc tcagaacttg cattgtcctg tctaaaggta
     4321 catcacacac acacacacac acacacacac acacacacac acacacactt atccaaaaaa
     4381 cactagcttc tcaggacata cgccctagag aggccactga ctggggatga agaggtcact
     4441 gccttgtttc ttgtgccgcc tcgctcaggc cacagtcaaa tccgtgggtc cccttaacgc
     4501 tccagacttg atctttccca cggttcagct ctcgcctccg aattaggcct cactctggtc
     4561 tcttttccaa aagacatcag tcctaacctt ctgcaggccc agtaggccca gaggcaagga
     4621 gagaattgtg gtgttctaca tggcgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     4681 gagagagaga gagagagaga gagagagaga gagagagaga gagtgaagag acctagaacc
     4741 caagcaaaac accctgaaaa taaaatttta aatgcttctt gtactaggaa aaggattccc
     4801 tacacatcca cgtcttttct ccactgggtt atgctgccat tttgtgggat gaggaaggga
     4861 aattaggagg aagaaagggg atgtagatag gatcccggtt cggtcaagta gttggaataa
     4921 ttgtggacaa gctcaggaga aggctttcaa ggcccaacat ccaaactcca ggccacgcag
     4981 cccagctggc cctcctccca ccggtcggtc cctgcccccc ttggctaatc atcttcatta
     5041 gaggcgtcat cgctgaccta atgaaagcaa actgtgtgga aatcgccggt aaatagttag
     5101 gtctgtgctg taattaattc agtgtctctt ttaatcaaac tggaaaagaa tcgggccgcg
     5161 ggttgtagag acgtgacatc acccccaaaa tcggcccgaa ccaatcagcg tcatcactcc
     5221 ggggacacgt gggtgagtcc cctttaaaaa aaaaacacaa cacaacgaaa aaaaaaaagg
     5281 agagagagag agagagagag agagagagag agagagagag agagagagag aaacaacaac
     5341 aacaacaaaa aagccagtcc taaagtaaaa gtgacccaag ttcgattttt ttttttttaa
     5401 aggaagtggg ggtggcggcg aaggcgcgcg cggaggacag gtgagagctg cggtgggagc
     5461 cgcagggcgc tgtccacggt gctggcgggg ccgagcagga gctccggggc caggtggggc
     5521 tgctcgatcc cggggccgct gtcgctgtcc gcggtgctga ggcctccagg tgcgctctgt
     5581 tctgcctgcc cgtaacgcgg ccccggccgg ttgctatcgg ccgtgctgac ccctgccctg
     5641 ggctaccgtt tgcctggagg aagcgtgcgg tttggatttt gttttttcaa aagacgatct
     5701 ttattcagat tccatcaacc tcgtgtttct tctgtgccta agggatcctc gggccgttct
     5761 tctacccttt ggctctcccc aacttggcgc gccctcctcc cattcctcct gacactccca
     5821 ccaccacccc ccctcggctc ggcggctcgg cgcggtgctg cagaagacgc gttgagccct
     5881 tttggatgta atgcgcagag gaggttggcc cagagctccc gggctccccc aaggctgaac
     5941 tccgtccaag gtgcccacag gctccctgcc cgccttcccc atgccagccc gcagctaggg
     6001 gcaggggcag cggcggctgg ggttgggggt gggtggggag cttttgggga ggacaggttg
     6061 cagcttggct atggaaggct ctaatggctt tgggattgac tccattctct cccaccgagc
     6121 gggcagcccc gcccttccca agggggaccc cttgcttggg gactgccgtt cacccctgga
     6181 gctgagtcca cgctcagaga gcagtagcga ctgctcttca ccagcctcgc ccggaagaga
     6241 ctgtctggag accagtacct cgcggcctgg tgcagcatct ggcccaggtt tggactctca
     6301 cctgcaaccg gggcagcttt cagccccagc ccagtcgcga actgtcacct cctcctttct
     6361 gatcagggac atccttgctg actgcaaacc tctcgcggcc tgtgcaccct actctagcag
     6421 tgggcagcct gcagcccctg agcctggggg ccgccttgcg gccaaggccg gggaggactt
     6481 tcgagacaag ctggacaaaa gtgtcagcag cgcttcatca gactctgagt acaaaggtaa
     6541 gaaactaagg ggaaaagcgg ggtgggtggg ttctaaaaag tgacatgtaa aacttgagag
     6601 cacacacttg gagacaggaa catagtcatc tccaggatct ctcaggccgg ggccgagagt
     6661 tggttttcct ctagacctct ctaccagctc caaaggttga ggtcctggag caacaaagag
     6721 ttcagcgtgt gtggctcgga gaagctgggg aggatactgt gtgtggagga gagcaagcct
     6781 ccccaggtgt acaaagcaaa ctctggcaag agcctcacct tctgaggatc cctgggagga
     6841 gagcttgcaa cagtgcatag cttggaggct gctgcactgt tggagtgaga gcctggtagg
     6901 aaattcccat cagagccctt agtacagtac aaattgtcta gcttttaatc tgaattttcc
     6961 caaaagagga aaaggtgggc ctccccaccc attgagtgtg gtgtagtcta ggcctgcgaa
     7021 ccatgcagaa agagagaggc caggtggctg gctggattag ggtctgaggc tgaggcggag
     7081 ggggaagaat gccataccta cacagcagct ctctctgact tgggtcttca gtgggttgaa
     7141 cagtggtcac caggctgtgg ccaggaccca gagatttctg agtggttatc aagttagtcc
     7201 tactttctct ttgggaatct ctgtgatggc tccctcttca ttctgtctta acgaagggag
     7261 ctgaagtggt attaaatgga cggaggaaag gaagagaagg aaggaaagaa gaggggaggg
     7321 aggagagaag aggggaggga ggagagaaga ggggagggag gagagaagaa agaggttgct
     7381 tagttttgta tcttctcttt atggctctgc cttgacttta gctgcttatt gggtccagga
     7441 gctcaaagtt ctttcccagg agaagtgaag tggcagaggc cacagagtgt caaaggtatc
     7501 aggggatgga gctctcttgc caagcagcag aaaatcaagt cctggtagag actgctcctg
     7561 gacttgtaac tattacaact atagatcccc caagcctctt cctcaggtga atgctatgcc
     7621 atgaagacag cgtcattgct gcatgcacct cctggtctct atttgtgcta aatttcacaa
     7681 aatttagtta gagtctcttg agtcttcttt tcaaccaaga attcctctga gggtagaccc
     7741 aggctccaaa ccgaagccca tcaggtcctg gtctcctttc tctcttcttc ccttagtaga
     7801 aatggctagg cggccttcaa ccaggctgtg gaaccacaat atctagaatg ctcccagttc
     7861 caaactcagc acgcagaaga aggtgttttt ggaaacttgg cccttttgcc tcccccttcc
     7921 caggggcctg tttagagagg caaagagaac tcaccttgga gagaggtgtg tgtgtgtgtg
     7981 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgattactta caatttgtaa ccatctctac
     8041 ctagtttcat gattagggca aagagccatt gggtccagca ctgctgtaca aacaagttac
     8101 ttccttttat ggttttgccc ctaagcagga ttttgaggag agattccatt tcgtttaaga
     8161 taattggaat atcaattttc ttctcctgtc cctgatgata tctgaggagg gagatgatga
     8221 gagagcggag agccggctaa gtatcactta aacgggtttg gagcagagat gctgtagaaa
     8281 gttccagcca cccctaggca ctggctgttg cttttgccct taacaaaagt ctctgaagtg
     8341 aatggttgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgagagagag
     8401 agagagagag agagagagag agagagcatg cctagcacac atctcaaaca ggggtgactt
     8461 ttgcaaagtc tgacttagga agctaaactg agagacagac acaggagcca gcccagaggg
     8521 gcatttaagt ccttcaccac agccacaaac cactgagtca ttttgttctt gcaggcaggg
     8581 gtggggagca ggccagtctc atcacaagtt tctggtgtga aagatttctt aaacagtaat
     8641 tactgaagtt gaacagggct ttggaggtag cgcgggtggc aacagtactg gagctctggg
     8701 ctcttagaga aaccaaacca ggcttcaccc caccaaagat ctctggtccc caggctctag
     8761 tacgatggag atggagtgcg gctctgaggc cgaactctaa aaaattggac acaagataga
     8821 cagccagaac tgcaggagtc ccgaaggcgc cagcagagct cggaaaggaa gaaagtctaa
     8881 aaaacaaaca accgaccact ccagcgggat ggccgattgt tatcaatagt ccaaataatt
     8941 agggggtaaa gtaagatttc tgcaatatca agtttgaggt ctgaataagg ataagtttta
     9001 ggctttcaag ggagaaaagt catttttgtc cctgcgggct ccaaagcctc ccagtggagg
     9061 cgaaagctga aggccgaagg ccgtctgggg cagttactcc gggactccgg cgctccggtg
     9121 ctagggcttc aggctccctc cctgtcgcga agcacagcca ttccggctga aaagcctccc
     9181 tccccagtgg ctcaattaac cggattattg tttcctacag agaaaaatca cctcacattc
     9241 agctgaaccc tgtcatggca aatgccccgc acaatgaatg aaactcaccg taaaagaaga
     9301 taaagaaagc aggggtaggg tgggcgggag ccccagtcta gagggttatt ttgggagaag
     9361 tggactattc tgttccaatc tttccttggg agatggagat gggaatgccc aacttgttca
     9421 attaaacgtg tgttttctgt taagtcaacc ccaacgaaaa tagaggaaaa cgaactaatc
     9481 tagctttgtc ctgtctacgg tggacctcag gagccgggaa cagatggcca tttgcccaac
     9541 cgagggctgt agcgagggtg ggttgggagc cacagtgggc acagcgaagc cgggacctct
     9601 gcgatgcacg cagcccttcg tgcccaggga caacataggt atttctcgca gctccgagcg
     9661 ctctcagatg ccgaccatgc cggctttcca tccgcttagt tcgaaactgg tctaggaagc
     9721 catctctgta gattaaggcg cataacgata ccacgatatc aacaagtttg tacaaaaaag
     9781 caggctggcg ccggaaccga agttcctatt ccgaagttcc tattctctag aaagtatagg
     9841 aacttcgaac cctttcccac accaccctcc acacttgccc caaacactgc caactatgta
     9901 ggaggaaggg gttgggacta acagaagaac ccgttgtggg gaagctgttg ggagggtcac
     9961 tttatgttct tgcccaaggt cagttgggtg gcctgcttct gatgaggtgg tcccaaggtc
    10021 tggggtagaa ggtgagaggg acaggccacc aaggtcagcc ccccccccct atcccatagg
    10081 agccaggtcc ctctcctgga caggaagact gaaggggaga tgccagagac tcagtgaagc
    10141 ctggggtacc ctattggagt ccttcaagga aacaaacttg gcctcaccag gcctcagcct
    10201 tggctcctcc tgggaactct actgcccttg ggatcccctt gtagttgtgg gttacatagg
    10261 aagggggacg ggattcccct tgactggcta gcctactctt ttcttcagtc ttctccatct
    10321 cctctcacct gtctctcgac cctttcccta ggatagactt ggaaaaagat aaggggagaa
    10381 aacaaatgca aacgaggcca gaaagatttt ggctgggcat tccttccgct agcttttatt
    10441 gggatcccct agtttgtgat aggcctttta gctacatctg ccaatccatc tcattttcac
    10501 acacacacac accactttcc ttctggtcag tgggcacatg tccagcctca agtttatatc
    10561 accaccccca atgcccaaca cttgtatggc cttgggcggg tcatcccccc ccccaccccc
    10621 agtatctgca acctcaagct agcttgggtg cgttggttgt ggataagtag ctagactcca
    10681 gcaaccagta acctctgccc tttctcctcc atgacaacca ggctcccagg tcccgaaaac
    10741 caaagaagaa gaacgcagat cgcagatcgc agatctctag agtcaccgag ttgctttacc
    10801 ggatgaagag ggccgaaaca tactgtccaa ggcccttgct ggcaatccac ccaactgaag
    10861 ccagacacaa acagaaaatt gtggcaccgg tgaaacagac tttgaatttt gaccttctca
    10921 agttggcggg agacgtcgag tccaaccccg gaccggtgca tgtggacccc aagaaaaagc
    10981 ggaaggtgga ccctaaaaag aaacgcaaag tggatcctaa gaaaaaaaga aaggtgcacg
    11041 tgtccatggt gtccaagggc gaggaactgt tcaccggcgt ggtgcccatc ctggtggaac
    11101 tggatggcga cgtgaacggc cacaagttca gcgtgtccgg cgagggcgaa ggcgacgcca
    11161 catacggaaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccttggc
    11221 ctaccctcgt gaccacactg acctacggcg tgcagtgctt cagcagatac cccgaccata
    11281 tgaagcagca cgacttcttc aagagcgcca tgcccgaggg ctacgtgcag gaaagaacca
    11341 tcttctttaa ggacgacggc aactacaaga ccagggccga agtgaagttc gagggcgaca
    11401 ccctcgtgaa cagaatcgag ctgaagggca tcgacttcaa agaggacggc aacatcctgg
    11461 gccacaagct ggagtacaac tacaacagcc acaacgtgta catcatggcc gacaagcaga
    11521 aaaacggcat caaagtgaac ttcaagatcc ggcacaacat cgaggacggc tccgtgcagc
    11581 tggccgacca ctaccagcag aacaccccta tcggcgacgg ccctgtgctg ctgcctgaca
    11641 accactacct gagcacccag agcgccctga gcaaggaccc caacgagaag agggaccaca
    11701 tggtgctgct ggaattcgtg accgccgctg gcatcaccct gggcatggac gagctgtaca
    11761 agcctgcagg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg
    11821 ggccgtccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc gatgcaacga
    11881 gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg ttttctgagc
    11941 atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc aagttgaata
    12001 accggaaatg gtttcccgca gaacctgaag atgttcgcga ttatcttcta tatcttcagg
    12061 cgcgcggtct ggcagtaaaa actatccagc aacatttggg ccagctaaac atgcttcatc
    12121 gtcggtctgg gctgccacga ccaagtgaca gcaatgctgt ttcactggtt atgcggcgga
    12181 tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca
    12241 ctgatttcga ccaggttcgt tcactcatgg aaaatagcga ccgctgccag gatataagga
    12301 atctggcatt tctggggatt gcttataaca ccctgttacg tatagccgaa attgccagga
    12361 tcagggttaa agatatctca cgtactgacg gtgggagaat gttaatccat attggcagaa
    12421 cgaaaacgct ggttagcacc gcaggtgtag agaaggcact tagcctgggg gtaactaaac
    12481 tggtcgagcg atggatttcc gtctctggtg tagctgatga tccgaataac tacctgtttt
    12541 gccgggtcag aaaaaatggt gttgccgcgc catctgccac cagccagcta tcaactcgcg
    12601 ccctggaagg gatttttgaa gcaactcatc gattgattta cggcgctaag gatgactctg
    12661 gtcagagata cctggcctgg tctggacaca gtgcccgtgt cggagccgcg cgagatatgg
    12721 cccgcgctgg agtttcaata ccggagatca tgcaagctgg tggctggacc aatgtaaata
    12781 ttgtcatgaa ctatatccgt aacctggata gtgaaacagg ggcaatggtg cgcctgctgg
    12841 aagatggcga tctcgagcca tctgctggag acatgagagc tgccaacctt tggccaagcc
    12901 cgctcatgat caaacgctct aagaagaaca gcctggcctt gtccctgacg gccgaccaga
    12961 tggtcagtgc cttgttggat gctgagcccc ccatactcta ttccgagtat gatcctacca
    13021 gacccttcag tgaagcttcg atgatgggct tactgaccaa cctggcagac agggagctgg
    13081 ttcacatgat caactgggcg aagagggtgc caggctttgt ggatttgacc ctccatgatc
    13141 aggtccacct tctagaatgt gcctggctag agatcctgat gattggtctc gtctggcgct
    13201 ccatggagca cccagtgaag ctactgtttg ctcctaactt gctcttggac aggaaccagg
    13261 gaaaatgtgt agagggcatg gtggagatct tcgacatgct gctggctaca tcatctcggt
    13321 tccgcatgat gaatctgcag ggagaggagt ttgtgtgcct caaatctatt attttgctta
    13381 attctggagt gtacacattt ctgtccagca ccctgaagtc tctggaagag aaggaccata
    13441 tccaccgagt cctggacaag atcacagaca ctttgatcca cctgatggcc aaggcaggcc
    13501 tgaccctgca gcagcagcac cagcggctgg cccagctcct cctcatcctc tcccacatca
    13561 ggcacatgag taacaaaggc atggagcatc tgtacagcat gaagtgcaag aacgtggtgc
    13621 ccctctatga cctgctgctg gaggcggcgg acgcccaccg cctacatgcg cccactagcc
    13681 gtggaggggc atccgtggag gagacggacc aaagccactt ggccactgcg ggctctactt
    13741 catcgcattc cttgcaaaag tattacatca cgggggaggc agagggtttc cctgccacag
    13801 cttgatgaag atctgagctc ttaattaatg atcataatca gccatatcac atctgtagag
    13861 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
    13921 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    13981 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    14041 ctcatcaatg tatcttatca tgtctggatc cgtttaaact aactttaaat aattggcatt
    14101 atttaaagtt acatatggcg gccgccctaa ggctcgacct cgaaattcta ccgggtaggg
    14161 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
    14221 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
    14281 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
    14341 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
    14401 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
    14461 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
    14521 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ttccggaggc
    14581 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
    14641 ccgggccttt cgacctgcat ccatctagat ctcgagcagc tgaagcttac catgaccgag
    14701 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
    14761 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccaga tcgccacatc
    14821 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
    14881 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
    14941 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
    15001 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
    15061 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
    15121 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
    15181 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
    15241 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc
    15301 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcgat gatatcagat
    15361 ccccgggatg cagaaattga tgatctatta aacaataaag atgtccacta aaatggaagt
    15421 ttttcctgtc atactttgtt aagaagggtg agaacagagt acctacattt tgaatggaag
    15481 gattggagct acgggggtgg gggtggggtg ggattagata aatgcntgct ctttactgaa
    15541 ggctctttac tattgcttta tgataatgtt tcatagttgg atatcataat ttaaacaagc
    15601 aaaaccaaat taagggccag ctcattcctc ccactcatga tctatagatc tatagatctc
    15661 tcgtgggatc attgtttttc tcttgattcc cactttgtgg ttctaagtac tgtggtttcc
    15721 aaatgtgtca gtttcatagc ctgaagaacg agatcagcag cctctgttcc acatacactt
    15781 cattctcagt attgttttgc caagttctaa ttccatcaga agctggtcga gtaactttaa
    15841 ataattggca ttatttaaag ttaggcgcgc cataacttcg tatagcatac attatacgaa
    15901 gttatgtcga gatatttaaa tattagaccc agctttcttg tacaaagtgg ttgatatctc
    15961 tatagtcgca gtaggcggag gaacaaatag ggttttgttt ctctacgcga gctttgcgcc
    16021 caaacgaggg agactatcct ccagaaatcc attctcggcc agtgacacat taggccggtc
    16081 tgcccctggc agtctttatc tccccctccc ccggtgtggg ggagtgaggc gggagaaaaa
    16141 gggttgagtt tcttcaggga gctgccagcg gctttggccc cgttcacttc ctacaccgtt
    16201 tcctcctcgc tcatagttca cacccttctg caggctggga atcctggctc cgatggccag
    16261 gccgtagcat ccccaagggc ttgggtgcta tgggacccca gaggtctctg ctctgacgac
    16321 tgggacccga ggaagccctg acatgctttc tgtgtctccg cagtgaagga ggagggcgac
    16381 cgcgagatct ccagctctcg ggacagtccc ccggtgcgcc tgaaaaagcc acgcaaagca
    16441 cgcaccgcct tcaccgacca tcagctggcg cagctggagc gtagcttcga gcggcagaaa
    16501 tacctgagcg tgcaagaccg catggagcta gccgcctcgc tcaacctcac cgacacgcag
    16561 gtcaagacct ggtaccagaa ccgcaggtga ggtttcgctg tggggcggga tcggttgggc
    16621 gcttctggag ccttgggtcg cccccaacct gcaaaagttg ggaaactgag gctctgagaa
    16681 aagactcact gtgccctaag tcccacagtg aggggcagaa aggacatcag cctggaatcc
    16741 aggacagaga tggcgcaacg caattaatga taacttcgta tagcatacat tatacgaagt
    16801 tatggtctga gctcgccatc agttcagaca gccccagtct ccagaaagca gagaggtccc
    16861 tgaggtggtt gtagtaggag ccagtggtga ctgcttatat ctcagagcat ttcttccctg
    16921 gacctcctag cacggacagc caagaagggg ctctctgcct cacgccagat cctctgggac
    16981 agagaggaat gggaaactgg tattaagaca ctggtcttga acctgcttct taatctcctt
    17041 cttggacatt cataggactt aaatagatat gtacaccata cgtacacaga cagcatagtg
    17101 acccacactt cccacataca tagcagaggc aggaatgatg ccttccataa acaagcctca
    17161 gaacacacag ccgcattaat aataaatacc gattactgct tcaggcatca ataattaagg
    17221 ctcgttgcag taattacaca attatgatga tgatgaatta ttcagggggg tggggacagg
    17281 gctagtgtga ctgagcccac ctcagcctca ctggaaccca ggcaggcatc cccaacctca
    17341 ctacaggtgg gtatagaacc tttaggtttg acttcctcaa accttgccct aggcctctca
    17401 acttctgggc caagtggggc tccgggctat cccaccacac gctgggagag tgtgggtagg
    17461 ctgggagaga aagagtgtac attcctttag agaaatcttc gcttgcagac ccagcctact
    17521 tgcttcaagt ccgagcctct ggggctacat gggactgggt aaggggtaag ggtggggcgg
    17581 gacacaggct accagtaggt agctccacca ctttcattag ggggtcttcc cagggcaagc
    17641 atacccagga gccctaccac aggagtttgt aagtgcgctt gggagtcaga caatctggct
    17701 tggagcaggg gctctgcttg ctactttgca gatcgtcttg aaaactagaa atacctcagg
    17761 cttttaatct gtaaaatggg gacaaaagtc atggaaagtc agtggaagag tatgttgcat
    17821 tgtgtatttg acatggatac tccattattt attaacaagt tagcatgctc cccgttggcc
    17881 atctcgcggc tctcggtgcc aatcactagg aaggggtaaa ggttgtgatc ccttcgtctg
    17941 tcttgatttc ggttcctagg actaaatgga agcgacagac ggccgtgggg ctggagctgc
    18001 tagcggaggc aggcaattac tcggcgctcc agagaatgtt cccgtcgcct tatttctacc
    18061 cgcagagtct ggtttccaat ctggaccccg gtgccgcact ctatctgtac cgcggaccca
    18121 gcgcgccacc tcctgccctt cagagacctc tggtgccccg catcctcatc cacggactcc
    18181 agggcgccag cgagccgccc ccgccgctgc ccccgctgcc cggcgtcctc ccgcgcgccg
    18241 cgcagccccg gtgaagctcc cgcttgcccc aaaccatgct ctgggacccc cagctcaggc
    18301 tgggcgtggg tccctccctt cgccccgggg cagggctgct gggctcccct ccgcagccca
    18361 gtgtccccgt gtgggagaaa ttcactgagg ctacccctcc cctacgtcct ctctcttggc
    18421 tgctccatcc aggagctact tacactcgcc ggatgtacat atgcgtcctt gcccacctct
    18481 caccaagccc cggatccttg cggacttccc caggtgcccc ctgtcccctc agcagcaggt
    18541 aggggacgcc ggtgtcccag gcctggggcc cacccttctt gcacctctgc gggacagagt
    18601 gggagcgcta tacggagctt ccctcccatt tcctttattc ttcctaagcc ccgggctagc
    18661 agcggagctg tacatacagt gtgcaaagtg tatatgaagt tatttattcg tgacccatga
    18721 gcccgtgacc gtgtcagtga atcagtgagt ctgtggcctg tgccctcccc atcccgggtg
    18781 ggcaggaagg ggccaagagg gcttgcccac acatcctggc ccccagtccc gctccctcca
    18841 cccaggtccc agggcagcca gacctcttgg gttatctttt tttaaatgtg aaaataaact
    18901 tcttacaaat gactaggcgc ctgtccgcgc taccttccgt tggctcccgc tcaggccctc
    18961 cccactcctt cctccataaa taactttggc acctctggaa ctacagatcc tttcactggc
    19021 aagaagaggg agaattcgtc tccctcttgt cccgttcccc agttcctggg tgttgtagtc
    19081 atgcttagga gacttgggct ggactcccag gggtctcccc gtttccaccc cgaggctctt
    19141 ggccttctca tctgatttgt cccgtttcca cacggtctta ggaggtcacg ggcgaggcca
    19201 acgctcggcc tccctctgct gcgcgacagg ccgcgaagca ctcactgagc cgactcatct
    19261 ggctcaggcc agggcgccgg cagctggtgc ccggccctgc gggcgcgggg cagacagatg
    19321 gactccgagg ctgctccccg ttgccccacc ctcctccgcc cggcgccccc cgctctggct
    19381 acccgcagct cctagtctct aatgcctggt aggatcccat gggcctttgc gacgccacgt
    19441 taggcaaccc tttgctctct ctccatcctc cccttgttcc agtcctggga gaaataggag
    19501 caggggaggg ggcagatcaa ccacccgcct ctggctctga gatttcaggg gagtcagact
    19561 ccagatcacg ttcaaagggc caaggtcatc tcctgacact ggtccgtctc ccgggacctg
    19621 ggcgcaggtg ctcccctccc ccacaccacc tgcagcacac tgacctgcag atggaagaaa
    19681 tgcttgggag tttggatact tgcaagccaa gcaccttccc agcttttctg gcctaacctg
    19741 agagaacaag gcagtggagg gagggggcgt gttcagaaag acaccccccc aaccattttg
    19801 gctactgttt cctcccccac ccactagcat cctcgcaccc aggcttttat tttctgggat
    19861 ggtggggcta ttattctaat tttgcaaaat gcacagcagc taattaagtc aaggagcccc
    19921 tcggctttca agttcttttg catgtgaatg caggctggta aatccctcga tctggctgtg
    19981 gtggggtgtg agggggactg tccccaactc agggttaatg aagtgggaaa ggagtctctc
    20041 atagcctcct gcaaacaaaa ccccccaagc aacaaaccct gcccaaatcc tacacggatt
    20101 gacttaaacc caggggataa gtgctttaaa ttaatctcat tgaataagaa tgagaccaaa
//