LOCUS       255104#pL1L2_GT2_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        23197 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT2_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 255104
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000028985
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7510..7556
                     /db_xref="ENSEMBL:ENSMUSE00000169983"
                     /rank="1"
                     /note="ENSMUSE00000169983"
     exon            8385..8631
                     /db_xref="ENSEMBL:ENSMUSE00001289120"
                     /rank="2"
                     /note="ENSMUSE00001289120"
     exon            8822..8981
                     /db_xref="ENSEMBL:ENSMUSE00001288017"
                     /rank="3"
                     /note="ENSMUSE00001288017"
     misc_feature    4275..9978
                     /note="5 arm"
     misc_feature    10006..10030
                     /note="B1 Gateway"
     misc_feature    10043..10090
                     /note="Frt"
     misc_feature    10091..10590
                     /note="mouse En2 intron"
     primer          complement(10159..10180)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10055..10078)
                     /note="FRT3"
     primer          14249..14267
                     /note="seq primer NF"
     misc_feature    14309..14316
                     /note="RE sites"
     misc_feature    16109..16142
                     /note="loxP"
     misc_feature    complement(14317..14348)
                     /note="Rox"
     misc_feature    complement(16069..16100)
                     /note="Rox"
     misc_feature    14349..14370
                     /note="polylinker"
     CDS             14929..15525
                     /note="puromycin N-acetyl transferase"
     misc_feature    15044..15049
                     /note="Mutated RsrII site"
     intron          10591..10965
                     /note="mouse En2 intron"
     misc_feature    10963..10965
                     /note="Splice Acceptor"
     exon            10966..11012
                     /note="mouse En2 exon"
     primer          10866..10887
                     /note="seq primer FCHK2"
     misc_feature    11013..11135
                     /note="1Dleader"
     misc_feature    11136..11187
                     /note="F2A"
     misc_feature    11283..11999
                     /note="EGFP"
     misc_feature    11193..11282
                     /note="3X NLS"
     misc_feature    14065..14303
                     /note="SV40 polyadenylation site"
     misc_feature    13098..14042
                     /note="ERT2"
     promoter        14381..14892
                     /note="PGK promoter"
     misc_feature    15636..16068
                     /note="PGK pA"
     primer          14123..14146
                     /note="LRPCR RAF5"
     misc_feature    12009..12062
                     /note="T2A"
     misc_feature    12059..12059
                     /note="A to G to kill RsRII site"
     misc_feature    12063..13088
                     /note="Cre"
     misc_feature    10981..11013
                     /note="FCHK lf2a_lacz_gt2"
     misc_feature    10981..10996
                     /note="FCHK QC prefix region"
     misc_feature    10997..11007
                     /note="FCHK QC critical region"
     misc_feature    11008..11013
                     /note="FCHK QC suffix region"
     misc_feature    16165..16185
                     /note="B2 Gateway"
     misc_feature    9979..16215
                     /note="Synthetic Cassette"
     exon            16470..16577
                     /db_xref="ENSEMBL:ENSMUSE00001265520"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00001265520"
     exon            16885..17034
                     /db_xref="ENSEMBL:ENSMUSE00001266354"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 2 ENSMUSE00001266354"
     exon            18088..18166
                     /db_xref="ENSEMBL:ENSMUSE00001290792"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 3 ENSMUSE00001290792"
     exon            18468..18531
                     /db_xref="ENSEMBL:ENSMUSE00000999829"
                     /type="targeted"
                     /rank="7"
                     /note="target exon 4 ENSMUSE00000999829"
     exon            18893..18968
                     /db_xref="ENSEMBL:ENSMUSE00000169986"
                     /type="targeted"
                     /rank="8"
                     /note="target exon 5 ENSMUSE00000169986"
     misc_feature    16216..19081
                     /note="Critical Region"
     misc_feature    19105..19138
                     /note="Downstream LoxP"
     misc_feature    19082..19161
                     /note="synthetic loxP region"
     exon            19962..20136
                     /db_xref="ENSEMBL:ENSMUSE00000413170"
                     /rank="9"
                     /note="ENSMUSE00000413170"
     misc_feature    19162..23197
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggattcact gttatctatt tgttacctac tcaaaattga atgctgattt
     4321 ccatcctaat ccatcacaag ttgaagataa atgtcacaca tattaataat agtaatatgg
     4381 gctagagaga tggctccatg gttaagagtg cttactactc ttccagaggt cccagtttca
     4441 gatcccagca cccacagtat gtggcccaca actgcgtgaa gttccagctc taggagaatc
     4501 cggtgccttc ttccagcttc catgggcata cgtgtggtat atatacacat acacaaataa
     4561 aaataaatct ttaaaaataa gtaagcactc gacaagtctt ccacatctga ttaaatgcct
     4621 caccagcaac cccgtgacat acaaaactgt cacccagttt acacatgaac gttctgaaag
     4681 aagaagctat ttgcctaaat cacacagaaa ggaactggtc tcgtcagccc tgctaacatt
     4741 aatcgtatgc ccattaatca tatgcagaca ggtagatcac aaagctcaca ctcttaattg
     4801 tgaaccccca ccccaaggtg tccccttctt ccctcccttc taagacctcc cctcgacttc
     4861 ccttccaccc aaggggaata tgatgaaatc ggttcagtgg aactgagaag ctcaaacaag
     4921 gaagtgtcaa ctgctcagag atgagaagag ggccagagaa cttcttgtct ccatctttca
     4981 attcaaactc cctaatccga aatgcttcac cggggatttg caaccctaga caccatgatc
     5041 ctgaacattc cacttgatag cctttggctt acagggttct tcctgctctt gagtcaaaga
     5101 gcaaacacag gagggctagg ccacaaggct gtctgcttcc ttgggggata gcgtttgtct
     5161 gcattcctct tggcactatc tgggttgtct taagggctcc atcaagatgc ctctttgtgt
     5221 tttccaattc ctcttggcac cttctgggtt gtcttaaggg ctccatcaag atgcttcttt
     5281 gtgttttcca attctgtctt catattaaga ttcacagagc cagagaccca gccatcacac
     5341 ttgctcccag aagagcttgt ttggatacag agccctctga tgctcttggg gtccacagtg
     5401 agggatgctt aaaacagaaa tgtaattccc aaatagtcag gacttttgtc catgacctac
     5461 ctctcactac cttaagcctt tgctgccctt tggtgtctag gcaggttctg aacggggtat
     5521 gtaactagag ctataccact ttccccacac agtaacctcc tgcagacttg ggaggtgttt
     5581 ggggttaact ttaggtgcag tgacctagct caaaaagaag aagccttgat ggttccctta
     5641 tgtttcctgt tgctaacagt gatgtacctc acactctgaa gtccattaag gcagtagact
     5701 ttagcttgag ttattaacat gtcttttcaa ttcaaaaatg aatgaaacaa aagaaaaaag
     5761 tggaaaagga ctctttaaac tcaggacaga gtgtcaaaag caaacatcta actgtgtgaa
     5821 acaagagttc ccaccctcaa gactagatta gtctctatgt tggggtagag gtgggaggaa
     5881 tccaagagaa actgaagaga acacagcttc agtgcgtcca aaaggtatca cacactccct
     5941 ccaagacaga actcagaatg aatggactca ggaaacccca cagacatgtc aagaacagag
     6001 aatgcctatg cccacactcc tgcccagggc caagacaata aatgacccaa ggagagacag
     6061 tgagccagag ggcttaggtg ggaacagccg ctgggctaac agccactccc ccaccccaca
     6121 tagttctgcc tgaggcctat aaagggaagg ggcacagaca gtgttgtgtg tacaaggagg
     6181 aaaccataaa tgataaatgc taacttggaa gaagccaggt ggcaggtgac atccctgttt
     6241 ttctaggcaa gagcagggac aagcctctgt gtaccttggt acaagtaaac ccctgagaca
     6301 ggaatgaaga cttttctgta aatgaacaaa atattatcag aaatagcagg caaatctgaa
     6361 ggctgtgtgg acacctatgt tatcaagcaa gatgcgcacc agctccccaa gagtgctctc
     6421 cccaagaccc tgaggtgtga ggccatcttc atcttacaca gaaagaagga gcttccatga
     6481 tgagtggcct gaaaggtcag catacagcta ggagccaacc tgccatgttt tctgccataa
     6541 cccataccca gcatcctgac ttcagagatt tttgttcacc gaacacacaa tcccaggtct
     6601 tttccaggcc ttacctacct ccatttaccc aaagaccctg tgcagagcaa gacctactct
     6661 cagacctgta ggcccagcac ccaggagtct tagcaggata tgctgaaggt cagcctgggc
     6721 ttcatagtga cttcaaagca atcccacact atatagcatg gcctggttgg tctcaattca
     6781 agaccttggc caatccctga cttgtttttt tttttttttt actctgcaaa atggacttca
     6841 caggtctagg tagtggagaa cactgcttca ttactttatc tgaggaaagg atagggctaa
     6901 gagaccagaa gctgcaagat ccctcaagcc tgccaaccta gaaagatgtt tccagaatac
     6961 agaagtggga agtgtagtgt gggaaatcaa aatggagagc aagtatggag aaggcctttg
     7021 tactcatata cacacatata aaatttaggg gtctgtcaag atagggaggg aaaatgtagt
     7081 ttgattccta acactgtggg aacgagagtt tgccaactgt agaatgaatg agcctggggg
     7141 ggggggggtg ggaggagagg tcacaagtga tgagagcaag acaaagtttc ctgagtgctc
     7201 tggctggact gcccctttca acactgaaaa cctgatcctc tcgccacttg tgagcatggg
     7261 tctccaacct atgcctcatg tgtgaaaccg tgaagtcttg agaaagcctc catctcaaat
     7321 gttcttattt aatttgtgga ctgagcagct tagctgactc acctgttaat atgattgaca
     7381 attatttact ttggagagcc ctgaaagaca cttctaaact acagaaattg gaattatctc
     7441 taagcccatc atccaatatc aagtttccca actgccattc tataaaagcg gtgattcttc
     7501 cttggcaaag tgataggaga ggagcccagg acgaccactg agaccttgag actcaggtga
     7561 gacctgcccc ttcctcctct ccacagatgc tccaggtagc agaaggccag gtctctcact
     7621 gtaggctggt gctggaagac tggactgtca ctaactgggg atcttataag aaaaagagag
     7681 ctggccatga cggggacagg gactggcccc ccgcattcat ctttcctgga gagggtgaca
     7741 ttgatagagg agtgggctta ctccatgtgt cctaaagtaa aagctctagg aagaaagttt
     7801 aacaaaatga ccatcatgga atgagaggct acctgtgctc gtgtgatatt caaatgccat
     7861 gatggcctga aaacagacca aagaaccatc cctgggcaga gatgagggcc gagcaggtca
     7921 ggtcctcagc ctctgccatg caggatcctc gtcatccaca ctgaagaaca gggctgaggt
     7981 caatgggtca aaccaccaca gtcacctccc acagccccag agtaaacctg acggtcactt
     8041 agttaaacag aagcttctgg gtcaggcagg aacagcttca gagcctctca cagctgcaac
     8101 tagtgacctt ggggaaactg cctagagtcg ctaggcctgt gctcctacca tgcagtaggg
     8161 gttgtctgtg cccatatctg aggatgtggt gacgatgaag ttggtggctg aacccatcag
     8221 cctgtggcct gcgacagacc actatggcaa tgttagtttt attgctagtt atcagcccct
     8281 ggggtcgggg gaacaatagc aatcccatcc tctggggaca gggcatacag tgcagagatt
     8341 cctctgatag ctgtaccata gagaacttgt cattctgtct gcagacacca agagagatgt
     8401 ttctagttgg gagcctcgtt gtcctctgtg ggctgctggc ccacagcaca gcacagctgg
     8461 caggcttgcc attgcccctg ggccagggtc cacccttgcc actgaaccag ggcccaccgt
     8521 tgccactgaa ccagggccag ctgttgcccc tggctcaggg tctgcctttg gctgtaagcc
     8581 cagcactgcc ttcaaatccc acagatcttc ttgctggaaa attcacagat ggtgagtcta
     8641 tgacagtccc tttcagggga tgttcaagtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     8701 aacatgtgtg cttgagtata tccaatctcg caataccagg aaggcaggta tgagggagga
     8761 aggggaggta aggacctgca gttggcagac atgatctctg aagctctttc cctctttaca
     8821 gctctcagcg gtggcctgct gtctgggggg ctgctgggca ttttggaaaa tattccactc
     8881 ctggatgtta taaagtctgg aggaggcaat tctaatggcc ttgttggggg cctgctggga
     8941 aaactgacgt catcagttcc tctcctgaac aacatcctcg agtgagtatc cacacagagg
     9001 gacttggttc ccactaacaa cagaagtcct aggcagccct acagagggag agggatgggc
     9061 tgagggtgga gccacgggtc atccccaggc ctctcttcat gctggcaggg acagtggccc
     9121 ttttgccctt gtagtggccc ccagtcctaa ggcacatcct gggccccagc agaagctatt
     9181 atccctggga caagccttcc atgtcagctt gtcccataat ccccagttct cagatggaaa
     9241 aaactgaggc acagacatgt gcctgtgctc acatagctag ggggatgcag tcaggaatct
     9301 atctcagaga agccctaagg tttgccaccc actgtgtcca ttccaccaag ggaatgtgaa
     9361 tggtcatagg ctaccctctc catctgtatc acctgtgcct gagacctgac atggtccttc
     9421 ccatgacagc ttcctaggca caaagccaca atggggaact tgttgcagtg accacatacc
     9481 caagcctgag tctttattaa gaatggaccc aagacactga caagattcct cgcctcccgg
     9541 gcctttgctt cctgacctag agatagacag acttcagtgc tggatgacct gcggtcaggc
     9601 acctggtctc ctggctttga tttctccatc tgtgagatgg agagcaagac tctggggaaa
     9661 accaggggca gtgaatagga cgccatggat gggtcagaga aggcttcctc ccaccctttt
     9721 gcattctttc caccttctgc tctttgatca ccttgataag aactgaagcc tgacctgcta
     9781 ctgaagatta tgacaaaact agtgttttgg gccctaagtc ctgggcaggg cttatcctag
     9841 ttgcccatgt atcttctatg tgtgtaccaa tagtccagca cagccagcca gccatctttc
     9901 ctccctttca tatacctact tgcacttctg tccacccttc tataaaccca actacccatc
     9961 tcaccttaac ctacagaaaa ggcgcataac gataccacga tatcaacaag tttgtacaaa
    10021 aaagcaggct ggcgccggaa ccgaagttcc tattccgaag ttcctattct ctagaaagta
    10081 taggaacttc gaaccctttc ccacaccacc ctccacactt gccccaaaca ctgccaacta
    10141 tgtaggagga aggggttggg actaacagaa gaacccgttg tggggaagct gttgggaggg
    10201 tcactttatg ttcttgccca aggtcagttg ggtggcctgc ttctgatgag gtggtcccaa
    10261 ggtctggggt agaaggtgag agggacaggc caccaaggtc agcccccccc ccctatccca
    10321 taggagccag gtccctctcc tggacaggaa gactgaaggg gagatgccag agactcagtg
    10381 aagcctgggg taccctattg gagtccttca aggaaacaaa cttggcctca ccaggcctca
    10441 gccttggctc ctcctgggaa ctctactgcc cttgggatcc ccttgtagtt gtgggttaca
    10501 taggaagggg gacgggattc cccttgactg gctagcctac tcttttcttc agtcttctcc
    10561 atctcctctc acctgtctct cgaccctttc cctaggatag acttggaaaa agataagggg
    10621 agaaaacaaa tgcaaacgag gccagaaaga ttttggctgg gcattccttc cgctagcttt
    10681 tattgggatc ccctagtttg tgataggcct tttagctaca tctgccaatc catctcattt
    10741 tcacacacac acacaccact ttccttctgg tcagtgggca catgtccagc ctcaagttta
    10801 tatcaccacc cccaatgccc aacacttgta tggccttggg cgggtcatcc ccccccccac
    10861 ccccagtatc tgcaacctca agctagcttg ggtgcgttgg ttgtggataa gtagctagac
    10921 tccagcaacc agtaacctct gccctttctc ctccatgaca accaggctcc caggtcccga
    10981 aaaccaaaga agaagaacgc agatcgcaga tctctagagt caccgagttg ctttaccgga
    11041 tgaagagggc cgaaacatac tgtccaaggc ccttgctggc aatccaccca actgaagcca
    11101 gacacaaaca gaaaattgtg gcaccggtga aacagacttt gaattttgac cttctcaagt
    11161 tggcgggaga cgtcgagtcc aaccccggac cggtgcatgt ggaccccaag aaaaagcgga
    11221 aggtggaccc taaaaagaaa cgcaaagtgg atcctaagaa aaaaagaaag gtgcacgtgt
    11281 ccatggtgtc caagggcgag gaactgttca ccggcgtggt gcccatcctg gtggaactgg
    11341 atggcgacgt gaacggccac aagttcagcg tgtccggcga gggcgaaggc gacgccacat
    11401 acggaaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccttggccta
    11461 ccctcgtgac cacactgacc tacggcgtgc agtgcttcag cagatacccc gaccatatga
    11521 agcagcacga cttcttcaag agcgccatgc ccgagggcta cgtgcaggaa agaaccatct
    11581 tctttaagga cgacggcaac tacaagacca gggccgaagt gaagttcgag ggcgacaccc
    11641 tcgtgaacag aatcgagctg aagggcatcg acttcaaaga ggacggcaac atcctgggcc
    11701 acaagctgga gtacaactac aacagccaca acgtgtacat catggccgac aagcagaaaa
    11761 acggcatcaa agtgaacttc aagatccggc acaacatcga ggacggctcc gtgcagctgg
    11821 ccgaccacta ccagcagaac acccctatcg gcgacggccc tgtgctgctg cctgacaacc
    11881 actacctgag cacccagagc gccctgagca aggaccccaa cgagaagagg gaccacatgg
    11941 tgctgctgga attcgtgacc gccgctggca tcaccctggg catggacgag ctgtacaagc
    12001 ctgcaggcga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccgggc
    12061 cgtccaattt actgaccgta caccaaaatt tgcctgcatt accggtcgat gcaacgagtg
    12121 atgaggttcg caagaacctg atggacatgt tcagggatcg ccaggcgttt tctgagcata
    12181 cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag ttgaataacc
    12241 ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta tcttctatat cttcaggcgc
    12301 gcggtctggc agtaaaaact atccagcaac atttgggcca gctaaacatg cttcatcgtc
    12361 ggtctgggct gccacgacca agtgacagca atgctgtttc actggttatg cggcggatcc
    12421 gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg
    12481 atttcgacca ggttcgttca ctcatggaaa atagcgaccg ctgccaggat ataaggaatc
    12541 tggcatttct ggggattgct tataacaccc tgttacgtat agccgaaatt gccaggatca
    12601 gggttaaaga tatctcacgt actgacggtg ggagaatgtt aatccatatt ggcagaacga
    12661 aaacgctggt tagcaccgca ggtgtagaga aggcacttag cctgggggta actaaactgg
    12721 tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc gaataactac ctgttttgcc
    12781 gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag ccagctatca actcgcgccc
    12841 tggaagggat ttttgaagca actcatcgat tgatttacgg cgctaaggat gactctggtc
    12901 agagatacct ggcctggtct ggacacagtg cccgtgtcgg agccgcgcga gatatggccc
    12961 gcgctggagt ttcaataccg gagatcatgc aagctggtgg ctggaccaat gtaaatattg
    13021 tcatgaacta tatccgtaac ctggatagtg aaacaggggc aatggtgcgc ctgctggaag
    13081 atggcgatct cgagccatct gctggagaca tgagagctgc caacctttgg ccaagcccgc
    13141 tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg
    13201 tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac
    13261 ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc
    13321 acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg
    13381 tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca
    13441 tggagcaccc agtgaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa
    13501 aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc
    13561 gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt
    13621 ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc
    13681 accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga
    13741 ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc
    13801 acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc
    13861 tctatgacct gctgctggag gcggcggacg cccaccgcct acatgcgccc actagccgtg
    13921 gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat
    13981 cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagctt
    14041 gatgaagatc tgagctctta attaatgatc ataatcagcc atatcacatc tgtagaggtt
    14101 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
    14161 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    14221 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    14281 atcaatgtat cttatcatgt ctggatccgt ttaaactaac tttaaataat tggcattatt
    14341 taaagttaca tatggcggcc gccctaaggc tcgacctcga aattctaccg ggtaggggag
    14401 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
    14461 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
    14521 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
    14581 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
    14641 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
    14701 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
    14761 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcttc cggaggcccg
    14821 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
    14881 ggcctttcga cctgcatcca tctagatctc gagcagctga agcttaccat gaccgagtac
    14941 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
    15001 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccagatcg ccacatcgag
    15061 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
    15121 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
    15181 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
    15241 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
    15301 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
    15361 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
    15421 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
    15481 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac
    15541 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgatgat atcagatccc
    15601 cgggatgcag aaattgatga tctattaaac aataaagatg tccactaaaa tggaagtttt
    15661 tcctgtcata ctttgttaag aagggtgaga acagagtacc tacattttga atggaaggat
    15721 tggagctacg ggggtggggg tggggtggga ttagataaat gcntgctctt tactgaaggc
    15781 tctttactat tgctttatga taatgtttca tagttggata tcataattta aacaagcaaa
    15841 accaaattaa gggccagctc attcctccca ctcatgatct atagatctat agatctctcg
    15901 tgggatcatt gtttttctct tgattcccac tttgtggttc taagtactgt ggtttccaaa
    15961 tgtgtcagtt tcatagcctg aagaacgaga tcagcagcct ctgttccaca tacacttcat
    16021 tctcagtatt gttttgccaa gttctaattc catcagaagc tggtcgagta actttaaata
    16081 attggcatta tttaaagtta ggcgcgccat aacttcgtat agcatacatt atacgaagtt
    16141 atgtcgagat atttaaatat tagacccagc tttcttgtac aaagtggttg atatctctat
    16201 agtcgcagta ggcggtgcac tatcatgcac agtctctttc ctcaaatacc ttctagacct
    16261 gagatagata gaggaccttg tgtattaggg ttcaattcag gcagaattga aggaagatgg
    16321 gtgtgctgga aaaactctct tcctgtcttc cttacctcag gtagccaact ctctctgaga
    16381 tgggtaaatt tgtgtgggga aaaaaattgt ttccaggttt aagacttgag accctgattc
    16441 tttcccctct aatgttgccc cctgaacagc ataaaaatca ctgatccgca gctgctagaa
    16501 cttggtcttg tgcagagtcc tgatggccat cgtctctatg tcaccatccc tctgggcttg
    16561 acactcaacg taaatatgtg agtgggtccg aagggggtgg caaggggatg gctcacaagg
    16621 agagcttggt ggtgatggga agctgcaagt gcctaggtct cgaaccttag cacagcggct
    16681 ggtccattga gcaaacttag ccgtgtagca aatggctaga cctctctgaa ccttgctctc
    16741 tcctacttgg gagatgagga caacggtgaa aaatataggg taaggtgagg ctggcactcc
    16801 taataataag aaacctcctc aaccttgaaa tgcaggagag ggacagatgc tagctagtaa
    16861 tgatgtctcc tccttttctg gcaggcccgt agttggaagt cttttgcaat tggctgtgaa
    16921 gctgaacatt actgcagaag tcttagccgt gaaagacaat caggggagga ttcatctggt
    16981 tcttggtgac tgcacccact cccctggcag cctgaaaatc agcttgctca atgggtgagg
    17041 acaaagggct agcttctccc acccagagac agcagtgtgg gatgacgtgt gtggacagtg
    17101 ggtggatgag taagaggata gaaaggaaga tgggtacgtg tggaggagga tgggttaggt
    17161 agattgacga atgtgtgctt ggtgagtgat ggaaagacat actgacatgg acagacagat
    17221 aagcaaatgg atacataaat actcacttat accttattaa acagaatggc cgactttttt
    17281 aatgctgtga gaaagcaaca gtgtatatgt tgtggctttt aatggcatta atactttttg
    17341 tcggtatggc caacttcatg gacctccaca gatattctat ctaaaattat cttacctaat
    17401 tattaccaag agacaaagtt tattactatg aattgctcca aaactatgct agatggtgtt
    17461 aaggaacatt atcctgttta ttatagactc atatttataa tataatggcc aattttataa
    17521 aggattagac aatagtgtgt atataagccc cacctactac agagtgtgtg aacttcactt
    17581 gtatttctcc aattttgtct catcttattt atttgtaata caaaactaca ttgaggatat
    17641 caacagaatt ctaaggggta aatagatgta gaggccattt caattgcttc ctctagttgc
    17701 ttgtcacaag ggaaggtcca gtcatacata gtgtacagga cctgtcccac atgtgtctca
    17761 catttactaa acacatacat tgggttttgt gggtatcatt tcattgagcc cccatgagga
    17821 ccacatacta cgcatcattc tgcattcatg gattccgtag tcttgttgta tgtctactct
    17881 gtccctgaaa gcatttgttt gtctgtgtat acacagctgg gttgggtgta atgcttcatc
    17941 taggctcctt gctgttcatc tgcatagaca tgagtgggga ggcttggctc agttttgggg
    18001 tgccaagact gagtgtgcgt agtggatgcg aatttcccaa gcccaacccc aagcctctga
    18061 gccacatcca tgacttttct cttccagagt cactcctgtt caaagctttt tagacaacct
    18121 cacagggata ttgactaaag tccttcctga gctgatccag ggcaaggtaa aggatcagaa
    18181 gaagggccaa gccccttctc cacgggtctt aaagcttctc tggctaaaag agagccctgg
    18241 tgtgaactaa acccagctac cctgtatggg aaatgaagcc atccattaat ccatctatcc
    18301 tgtgattcag ctgtctaagt tacttatttc tacacatcca aacatactgg ttggtttttc
    18361 tgggctgtag aaagtatcca cggacattcc atcttgggca tgctgcctct tcaaaagtct
    18421 cttcgcagga ggttctgaac ataactcctc tctctgaccc gggtcaggta tgtcctctgg
    18481 tcaatgggat tctcagcggt ttggatgtca ccctggtgca caacattgct ggtaagtcct
    18541 ctcccccaag gagttttgtc cctgcacatc atgggacagc tgggcctcac tggcttcatc
    18601 cctccttctt tggatatatc cttgtttcca aaaattatcc aggtgagtct gagataaagc
    18661 ctatctcctc ctcctcctcc tcctcctcct cctcctcctc ctcctccttc tcctcctcct
    18721 cctcctcctc cttctcctcc tcctcctctc agcctgacct gagaacagct gagctggctg
    18781 gaggagacgc agcctttccc caacattcct gggagtggtg agcatgcctg actccttcac
    18841 caccagaata tgcctctaat attacttttt tgcttttgtt gttgttgtcc agaattactg
    18901 atccatggac tacagtttgt catcaaagtt taggcatccc aggaaggaag gctatcttgg
    18961 ctgagctggt gaggactgct ccacattcct tcatatttga ggattggcca ttatccttct
    19021 agaaggaaac aaggatctag gaacaatgtc atcagtatta gtcagctatc tatcactgta
    19081 agagatggcg caacgcaatt aatgataact tcgtatagca tacattatac gaagttatgg
    19141 tctgagctcg ccatcagttc aacctaagat aatcaactta taaagataaa attgtttctt
    19201 ttggctcaca gttttacaaa cccagttcat gaccaatcgc cccattgctt tgggaacgta
    19261 ataaggcagg ggttatagtg gaagaagaca ttcatttagt agccaagaag caaaatgaga
    19321 gacagaaaga ggtagaattc tacagttcac cctgagggca tgccccaatc acctaaagat
    19381 ctccccctcc ctagtacctc actcctacag gatctactat ctctcagcat tgccatcatg
    19441 gggatcatgc cattaacatc tgtgccctta ggggaacatc taagattcaa gtcacagaaa
    19501 tatctaaaga gagacaagct tccacttgaa cacacagaaa tacacacaaa ggtccacagg
    19561 aaagctaacg aggaagagag acacaggtgc catagtaaca caacgacaac gagaaataag
    19621 catcatcatg gatgcataca tatcatcatc atcatcatca tggattcaca cacacgcaca
    19681 cacacacaca catacacaca caccagatac atattcgata caaacccaca cagtctcaaa
    19741 tcactatgct tatacatatg tgcaaacata cacatattca ctacagtcac atctccatag
    19801 tgcaagcaca tgcacaaaca gtcaggctcc ttatggctga gatcctatcc ttgggtctct
    19861 gcatgcctcc ttcccctaat cgggccagtt tgatgatggg acaataccag taagagtcac
    19921 tgacctcgtt tcgcctcctg atcaaattct tctcccaaca gaatcatttc ttgctgctca
    19981 gtctcctgcc tcttgcccag tctcccatgg ctcacagaaa ggggcccaca tcctggaaaa
    20041 ttatgtcttc cttctcctca cggagcctga tctcttccca tcaggcacga ttaatcctgt
    20101 gatcctcact aaataaaata gctcttcatc tgcactaatg tgttgtgtcg tcaccattac
    20161 ttgggacttt agaggctaga gagagcctga ggccataccc aacactcttg ggcctggtat
    20221 ctcatatact acgtcctaaa cagatgaggg ctggggtcca actgaatgtg gattaggggg
    20281 gtgaccctgc tgttcttagg tggtgtggat aagcacgcag gcattgattt acacacacag
    20341 gttgccacac aagacgtgat gcagatgaca ggagatatgt tccagaggtg ggcctgagat
    20401 gatagggtgt gaggatattt atgtctctct ctgatccaca tctcaggtta gggattggga
    20461 ggaaccagga atgtggaaag gtgaactgga tggagcagga agagcagacc tggatggtgg
    20521 ccactctgtg tggagatgct tcagaggccc tgaaggagac acttgacact gtcacaccag
    20581 cctcttttct gttctcccag caacaggagg acagagaatc acaaagaaag agagtgggag
    20641 ggaaggcaca gcagtccaag tgctacctgc ttgtggaaag cattgaagag gctaattgga
    20701 gttgtcatgg tttgctttgt tttattttat gttttcaaag gaaaaacttg attttgagga
    20761 agcaagaaga ggaggggtga agctgagatt gtatagaatg gtgacaaact gaattcacta
    20821 aaagagtctc tgggaggcca gaaaatcaaa caaacaagac aaaaacaaac aagacagaaa
    20881 caaacacaca aaccagtcca gtggactcca gagcagggaa gagggtagag caaccccaag
    20941 cctcttcccc agagcaatag caacaatgtg gcctgcccac taggtccctg tacaaccgct
    21001 cttaaacttg tcttcatctg atctggctca gggttcacag actaagaagg tacaacttct
    21061 cacatgtttg ctgaaaacca atcagaagtg gttgcttagc accagctgtc tggggtgctt
    21121 aggaccagct gcctggagtg gtgataacat taaagacaat gaatgtgcca atggtaaact
    21181 tccaaaggac aatctgggga atagaatgta ggcttcaaat atgttcagga ggccacactt
    21241 acatatgtat ggttttgggg gggggtgggt tcagaacagc ctaaggagaa cctatgctct
    21301 tgcctcctat gctcttgttc tgtgagagca agtgatatag atgaaggtaa ggtgcattag
    21361 ataagacagt gggcagccat tgctctgggt tcagtccttg tacaaggccc cagcaggtca
    21421 atctgaaaga gaactgactc actcataaga agcatattca ccaaggttgg aagccgcctg
    21481 ggtccactgg ttcccattgg ccattagttt tgcagaatta tcagtacatc aggggacata
    21541 tttgaatctt ggaccacatc tggctgttca gttcctcctg gtgagctggc tatttgatta
    21601 tggagtctac tagaccctag tgatcctggc tgggccagtc ttgcatgtgc cgattcattt
    21661 ttgaagatgg agttctgttg agtctgtcca tttgtattgg tggagctcca atcaagtgaa
    21721 agcagttcag gtcacagcat tactataaga tataccgtct ctttcagtac cgagtagcaa
    21781 gtaaagagct gccatttgaa gatatgtgta tgaaagtcac ttcaggcaac ctatgtgtcc
    21841 aacagcctag aaactgtgta ccccagggac agtttcctat tgccataagc accaatcagc
    21901 ataagcaaaa gatatgggca cctgcagtgt cactgggcca gcagtcccag aagccttaaa
    21961 gacaatgtat gggccttgtt gaatgtcttc caaggcctgc ttccacaaaa ggcatcattc
    22021 aaaatgggcg tttttgcaag ccaccttgat taggggaaaa caaagaaaaa ggcacctagg
    22081 tagacgacat gtagtcctta gttcttaaaa cccctagtct tatcatcagg cttggttgca
    22141 agcaccattt cctgctgagc tatctcttca gtccatgccc atattagtga atatcaccaa
    22201 ggacaaaaac atttgttcga tcaagaagag ggaagaccaa tgggtggata cttcattcct
    22261 ccttaaaata gggaacaaaa tacccacgaa aggagttaca aagtttggag ctaagatgaa
    22321 aggatggact atccagagac taccccaccc ataatcagcc accaaaccca gacactattg
    22381 catatgccag aaagaccttg ctgaagggac cctgttatag ctgtctcata tgaggctatg
    22441 ccagtgcctg gcaaagacag aagtggatgc tcacagtcat ttataagatg gaacacaggg
    22501 cccccaatgg agaaggtaga aaagcatcca aggagctgaa ggggtctgca accctatagg
    22561 tggaacaaca ttatgaacta accagtaccc ccagagctcg tgtctctagc tgcatatgta
    22621 gcagaagatg acctagtcgg ccatcacagg gaagagaggc cccttggtat tgcaaacttt
    22681 atatgcccca gtacagggga atgccagggc caagaagggg gagtgggttg gtaggggagt
    22741 agggcagggg gagggtatag ggaactttca ggatagcatt tgaaatgtat ataaagaaaa
    22801 tatctaataa aataaataaa caaacaaaca tttgttcaat tgtttgggag atgtgcgttt
    22861 ggcttcttgt ccatgcgacc cctaggaaat gtgccaacta ttcaagaccc cggacattat
    22921 ctggctttta gtttagataa ggagtaaagt cagtgctgat tctggttttt cagggtgccc
    22981 cactctcagg atgtcgtcaa tatactgggt gactagaact ttcccggtcc tgagcttgtt
    23041 gaaggtcaaa ccccacctgg aggtagcatg ctgcagaggt gtgaagcttc attcagaagt
    23101 actttacagg cagtaagagg taggcataca tccctgcctc ataaagagat ggagaaaatg
    23161 gcactaacca agccaataac agcatattaa tctctag
//