LOCUS       239328#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20725 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 239328
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000032835
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            6100..6176
                     /db_xref="ENSEMBL:ENSMUSE00000320917"
                     /rank="2"
                     /note="ENSMUSE00000320917"
     exon            8379..8762
                     /db_xref="ENSEMBL:ENSMUSE00000396270"
                     /rank="3"
                     /note="ENSMUSE00000396270"
     misc_feature    4275..9713
                     /note="5 arm"
     misc_feature    9741..9765
                     /note="B1 Gateway"
     misc_feature    9778..9825
                     /note="Frt"
     misc_feature    9826..10325
                     /note="mouse En2 intron"
     primer          complement(9894..9915)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9790..9813)
                     /note="FRT3"
     primer          13991..14009
                     /note="seq primer NF"
     misc_feature    14051..14058
                     /note="RE sites"
     misc_feature    15851..15884
                     /note="loxP"
     misc_feature    complement(14059..14090)
                     /note="Rox"
     misc_feature    complement(15811..15842)
                     /note="Rox"
     misc_feature    14091..14112
                     /note="polylinker"
     CDS             14671..15267
                     /note="puromycin N-acetyl transferase"
     misc_feature    14786..14791
                     /note="Mutated RsrII site"
     intron          10326..10700
                     /note="mouse En2 intron"
     misc_feature    10698..10700
                     /note="Splice Acceptor"
     exon            10701..10754
                     /note="mouse En2 exon"
     primer          10601..10622
                     /note="seq primer FCHK2"
     misc_feature    10755..10877
                     /note="1Dleader"
     misc_feature    10878..10929
                     /note="F2A"
     misc_feature    11025..11741
                     /note="EGFP"
     misc_feature    10935..11024
                     /note="3X NLS"
     misc_feature    13807..14045
                     /note="SV40 polyadenylation site"
     misc_feature    12840..13784
                     /note="ERT2"
     promoter        14123..14634
                     /note="PGK promoter"
     misc_feature    15378..15810
                     /note="PGK pA"
     primer          13865..13888
                     /note="LRPCR RAF5"
     misc_feature    11751..11804
                     /note="T2A"
     misc_feature    11801..11801
                     /note="A to G to kill RsRII site"
     misc_feature    11805..12830
                     /note="Cre"
     misc_feature    10716..10755
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    10716..10731
                     /note="FCHK QC prefix region"
     misc_feature    10732..10749
                     /note="FCHK QC critical region"
     misc_feature    10750..10755
                     /note="FCHK QC suffix region"
     misc_feature    15907..15927
                     /note="B2 Gateway"
     misc_feature    9714..15957
                     /note="Synthetic Cassette"
     exon            16380..16554
                     /db_xref="ENSEMBL:ENSMUSE00000201271"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000201271"
     misc_feature    15958..16717
                     /note="Critical Region"
     misc_feature    16741..16774
                     /note="Downstream LoxP"
     misc_feature    16718..16797
                     /note="synthetic loxP region"
     exon            18287..18414
                     /db_xref="ENSEMBL:ENSMUSE00000201259"
                     /rank="5"
                     /note="ENSMUSE00000201259"
     exon            19712..20005
                     /db_xref="ENSEMBL:ENSMUSE00000201288"
                     /rank="6"
                     /note="ENSMUSE00000201288"
     misc_feature    16798..20725
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatgttca gagagcgagg gaagaaacac aactccgaaa catacacaag
     4321 acttccaagt gaagggtgtt ctgagggagg aacaggctag ctttgtgtct gggtcttggg
     4381 taaaggtccc tctgcaaaca cacctcaggc tgatcttcct tgaccctcca ggaaaccatt
     4441 tcctagccct gactggttca ctgatcttga acaagaaccc ctctccaact ctgtgtctcc
     4501 tcaaagtgcc agacctgggg ccccagaaat ttattttttt aagttagtat ctaagtcata
     4561 aaatccctaa ggagaagcaa ggggccattt ctcagaactg tggaactttc cagacttcct
     4621 acagcacaag gggtcatgtg cctctatgcc ctcagccgca cagacaggcc tttcatttct
     4681 caggcctggg tcgaggactg atggtcaaaa atgccaagct ccagagacag gaaagggtcc
     4741 ccttgtcctg ggcccagact gttcctcccc aggaacccct ggatggaaat gtcagtgtct
     4801 cccctcccca caatcttgat gctcctacgg gtgctgcgcc ctgatccctg cgctggccca
     4861 gcctatggtg actaaacaga gagcgacacc tgctggacgg ttggactcca acagagctga
     4921 ggctgggatc tggaggccag ggggaagggt gctgtctcca tgaaggatgc tctctgtggc
     4981 tgcagatccc atctgtcctc taccactgcc ccatccccaa ggcttccttt attattctta
     5041 gaccaaaagt aaatgctgtt gctgctgctg ctgctgctgc tgctgcatgt ggggcatggt
     5101 gtcacacagt gcaggtctca gaaggtgtgt gagtggcatg gttgtgtacc ctccagggca
     5161 gtggtaccat gaggtccaga ttgtttccac atacagctgg ctgaaggcca ctgctccaga
     5221 tgatggcttg ataaattcag ttatgcttgc tggctggagt tggggatccc tactgttcaa
     5281 gggagagctt taagtggtgg ttgcaacaga tctgtcatta ccaagcctca tgtactatat
     5341 acataattat gttccccata ctttcatatg attggcatgc agtcgattgg tttgcacatg
     5401 tgtgctccac tactgaccaa tcatttcatt tcagcagaag atatgccctt gcatgactct
     5461 gtgtgtgtgt gcgggggagg ggggggagag ggagagaata aagtcaatac aagtggaggc
     5521 tgtgggtccc cagagttgca gagactcaaa aagaggccag agcacctggc tacacatctt
     5581 cagtttgaaa ctccacatgt tcaaacccca gaaaaagctg atgcttcagg tcaagtccaa
     5641 aagggaggag aaaatcagtt ggaaagccat gaggggagaa gtctctctcc ctctagaaag
     5701 ggtccttttt atgttaaagc cttcaggtgg ttggctgtga cccacccaca ctgcagaggg
     5761 gaggccctct aggtctacag attctggggt ttatgcaaaa cccctcacag tgtataaacc
     5821 tgagcatcta tggtccacct ggcacatatc agtagctatc actgggattc attaggacga
     5881 gcctccactg gaagatggga gaaccaatgt accaaccacc ttggaactaa tctaagaaag
     5941 gatgacggtt tctgaggaaa ggagccttag tggaaacact gtccaaatca tggcaaccca
     6001 cctgctgttc aatgtgattt cagagctttg gccatgaatt ttatcacggc atctcttccg
     6061 taactatcct gtgcctaaac tgtctgctat ctcttccagc tgaactatga ccactttact
     6121 cttggtcttt gtgactctga gggtcatcgc tgcagtgatc tcagaagaag ttccaggtga
     6181 gaacagttct atgcatgact cctttattca gtgggtactg ggttccgaat acagggtaca
     6241 agttcgtttt gggatactgt ccctgctgcc ccttcttttg gccttttctt ggccctttct
     6301 agatgtagta ggaacacaca cacacacaca cacacacaca cacacacaca cacacacaca
     6361 cactgaatgc cctcagcaag gagagttgct cagcctcctt cacagaaccc ttggggaatc
     6421 tcatgcagaa ctactccaca gtgaatgggg tagagagaag acatggggag ccagaggggg
     6481 gcatggatgg gaccaccaaa ggggagacga gaacctgagg aacaacgttg aaaagactgg
     6541 tttcaacgtt ctagaagtga gggcacagcc ctggtgtgag ctccccaacc ctgacttaag
     6601 ggacatgtga aggacttggc acacagtgtg gggatctgga actcaagggc tgaacagtct
     6661 gtgagacctc ccaaacagtg ggaggggagg ggataagaga aggcttgctc atggtggcag
     6721 ggacaagagg aggcttgggc accctccctc cccacaaccc actgtatgcc aatgcaaaac
     6781 tccaggactt cagggtcatg gaaaaaaaaa ctttccgtgg tgaaatggaa agacactcag
     6841 tattttattc actcacatac tggcctgatt cacagctttt gaatatgtag atgtggttat
     6901 ctggacctcc aatgatgctc agagatctat gaatacccag accctagcca tcaccttggg
     6961 agatgtgtcc atattagcaa aacagtccac tagggttggc tgagagcttt gacaaccacc
     7021 aagttggatt caacagtggt ctctggctat cccctgagga ccactattac agagccaggc
     7081 tgtggacacc ggcgtcaggg ctgggcagca gatggaggtg tttgtggaga gatggtggaa
     7141 tctgtccaga catcaaaagg tccaggctgg actccagaca ttcctggccc ccctatcctt
     7201 accctgctat gtctctacca cccacaacta ttctaaacat ttttatttca acctgtagca
     7261 tcccttgaaa gggagggttc taaaatttta agacctgcca tgaaaggtgc tgtatagaag
     7321 actgtttctc tctctccctt tccaacagaa tttaggaaac actatgtctt ctctattgac
     7381 acactccata atttttatgt cgtctaatcc tacccaaccc catatttctg actctacagg
     7441 ccaaaaaata aatgcctgac ctcctattct ccccagtctt ctcgtccttg actttaaaag
     7501 tcttgccctt ctacaagttc cagttaaagc ccaaagcaga cctcccagaa tgggatatat
     7561 ctatcttgaa gtcctatgtc tcaagtttaa aggctgtgga gaattttgta attctaataa
     7621 ttattatgcc caatgtgtag gacagtatct gtctctatga gaaaaatatc tcaggaaaac
     7681 agcttgtctg tggcaaggca gaaatgtcat gcctgaaggt acagtggagg ggagaggttc
     7741 acctcacagt agcctggaaa cagatgtggg tgggggagag aaagagagag agggagagag
     7801 agaaacagac aaacacagac agacagacag acagacagac agacagagat aaagaggcag
     7861 aaagacacag agagacagag acagaatgac agagacaggg agtcagggag actgggggcg
     7921 gggctgcgaa caagatatcc cttccaaagt tgcatctgca ttgatttatc tccccccatg
     7981 aggcagcaca tccccatcca gtcacctctc ctaacacagc cataccttcc acacacgaac
     8041 cttttatgga gaactcatga tatccaaaat aaatacttca atatattttt ctaaaatctg
     8101 tgaatcaagc atatcactac tctaagaata agggtcctgt gtatctaggg gttggcatcc
     8161 cctctgggtg tctaaggttt gaggtctctc tcatgctctt ctggaagtct ttgtttggct
     8221 ccggattttt gtcttagggt ggggagacac ctggagaatc cccaggttcc cacatggtag
     8281 gctggataag ggcctcttgg gtggctgcat ttccacgcct ggaaggaggg tctctatgat
     8341 tcctaacaca tgtctgtcct cccgcctttc tttcacagac catgacaact cactgagcgt
     8401 gagcatccct caaccatccc cattgaaggt cctcctaggg tcttccctca ccatcccctg
     8461 ctacttcatc gaccccatgc atcctgtgac cactgccccc tccactgccc ccctcacccc
     8521 aagaatcaag tggagccgtg tttccaagga aaaggaggtg gtactgctgg tggccactga
     8581 aggacaggtt cgagtcaaca gcatctacca agacaaggtg tcgctcccca actatccagc
     8641 catccccagc gatgctacct tggagatcca gaaccttcgc tccaatgact ctgggatcta
     8701 ccgctgtgaa gtgatgcacg gcatcgagga cagcgaagcc accctggagg tcatagtgaa
     8761 aggtgaaggg cctcccactg gggacgccat gttgcctgta ggaagagcta gaatgatgtc
     8821 ctcagcaatg tgcaccttgg agactagctc tcccactcag ttcctctgca tacccgtttt
     8881 ctccttatcg tgccctacta tgggactgtc tggcaaatgc tgttctctgt gggattggga
     8941 ccctaggagt ggggcacata aatacatcct ggtgttggca gcttgtttgc caacaatctt
     9001 tcacttgttg ggtctatcag tgtttcttgc tgctgtgatc agagacctga caggagtcca
     9061 cgtgagggag gaaggcgtta ctctggctca cagtgtgagg gcatacagtc caccatggtg
     9121 gggacaagat ggtggtagga gtctgaggca gctaatcata cggcatcgac agtcaggagg
     9181 caggaagatg aatcccactg ttcacccaga tcactctccc tttttttctt tcactctggg
     9241 accctggacc atgggatggt accatctacc ttcagagtag gtcttccctc ttcagtaaaa
     9301 ccctcacaga catgcccaca ggtgtgctcc taccccacag cagccttgta aatgtgccca
     9361 ttttgcacat cagcaagtga gttaagtggc atatctgcca tgatcctaga aatgagtgaa
     9421 agtgggttcc taacacgtca tccaggcatc atccccgccc gctcctccaa cctttattct
     9481 cagcttcaaa gaaatgatgg aagattcact tcctgttctc ctttgtctct aagaggtctc
     9541 atcaggatgc ccctgagcca ctggagacct ggaaccctca tatctcattc cctggattgg
     9601 tggtggagag gggggattag aaggcctgga gtgacaaaag gaaaatcaca aggagatagt
     9661 ttttgctatc gttccaataa ctttcttatt gtcagaatta ttaaatgtgt tttaaggcgc
     9721 ataacgatac cacgatatca acaagtttgt acaaaaaagc aggctggcgc cggaaccgaa
     9781 gttcctattc cgaagttcct attctctaga aagtatagga acttcgaacc ctttcccaca
     9841 ccaccctcca cacttgcccc aaacactgcc aactatgtag gaggaagggg ttgggactaa
     9901 cagaagaacc cgttgtgggg aagctgttgg gagggtcact ttatgttctt gcccaaggtc
     9961 agttgggtgg cctgcttctg atgaggtggt cccaaggtct ggggtagaag gtgagaggga
    10021 caggccacca aggtcagccc ccccccccta tcccatagga gccaggtccc tctcctggac
    10081 aggaagactg aaggggagat gccagagact cagtgaagcc tggggtaccc tattggagtc
    10141 cttcaaggaa acaaacttgg cctcaccagg cctcagcctt ggctcctcct gggaactcta
    10201 ctgcccttgg gatccccttg tagttgtggg ttacatagga agggggacgg gattcccctt
    10261 gactggctag cctactcttt tcttcagtct tctccatctc ctctcacctg tctctcgacc
    10321 ctttccctag gatagacttg gaaaaagata aggggagaaa acaaatgcaa acgaggccag
    10381 aaagattttg gctgggcatt ccttccgcta gcttttattg ggatccccta gtttgtgata
    10441 ggccttttag ctacatctgc caatccatct cattttcaca cacacacaca ccactttcct
    10501 tctggtcagt gggcacatgt ccagcctcaa gtttatatca ccacccccaa tgcccaacac
    10561 ttgtatggcc ttgggcgggt catccccccc cccaccccca gtatctgcaa cctcaagcta
    10621 gcttgggtgc gttggttgtg gataagtagc tagactccag caaccagtaa cctctgccct
    10681 ttctcctcca tgacaaccag gctcccaggt cccgaaaacc aaagaagaag aacgcagatc
    10741 gcagatcgca gatctctaga gtcaccgagt tgctttaccg gatgaagagg gccgaaacat
    10801 actgtccaag gcccttgctg gcaatccacc caactgaagc cagacacaaa cagaaaattg
    10861 tggcaccggt gaaacagact ttgaattttg accttctcaa gttggcggga gacgtcgagt
    10921 ccaaccccgg accggtgcat gtggacccca agaaaaagcg gaaggtggac cctaaaaaga
    10981 aacgcaaagt ggatcctaag aaaaaaagaa aggtgcacgt gtccatggtg tccaagggcg
    11041 aggaactgtt caccggcgtg gtgcccatcc tggtggaact ggatggcgac gtgaacggcc
    11101 acaagttcag cgtgtccggc gagggcgaag gcgacgccac atacggaaag ctgaccctga
    11161 agttcatctg caccaccggc aagctgcccg tgccttggcc taccctcgtg accacactga
    11221 cctacggcgt gcagtgcttc agcagatacc ccgaccatat gaagcagcac gacttcttca
    11281 agagcgccat gcccgagggc tacgtgcagg aaagaaccat cttctttaag gacgacggca
    11341 actacaagac cagggccgaa gtgaagttcg agggcgacac cctcgtgaac agaatcgagc
    11401 tgaagggcat cgacttcaaa gaggacggca acatcctggg ccacaagctg gagtacaact
    11461 acaacagcca caacgtgtac atcatggccg acaagcagaa aaacggcatc aaagtgaact
    11521 tcaagatccg gcacaacatc gaggacggct ccgtgcagct ggccgaccac taccagcaga
    11581 acacccctat cggcgacggc cctgtgctgc tgcctgacaa ccactacctg agcacccaga
    11641 gcgccctgag caaggacccc aacgagaaga gggaccacat ggtgctgctg gaattcgtga
    11701 ccgccgctgg catcaccctg ggcatggacg agctgtacaa gcctgcaggc gagggcagag
    11761 gaagtcttct aacatgcggt gacgtggagg agaatcccgg gccgtccaat ttactgaccg
    11821 tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc
    11881 tgatggacat gttcagggat cgccaggcgt tttctgagca tacctggaaa atgcttctgt
    11941 ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg tttcccgcag
    12001 aacctgaaga tgttcgcgat tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa
    12061 ctatccagca acatttgggc cagctaaaca tgcttcatcg tcggtctggg ctgccacgac
    12121 caagtgacag caatgctgtt tcactggtta tgcggcggat ccgaaaagaa aacgttgatg
    12181 ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac caggttcgtt
    12241 cactcatgga aaatagcgac cgctgccagg atataaggaa tctggcattt ctggggattg
    12301 cttataacac cctgttacgt atagccgaaa ttgccaggat cagggttaaa gatatctcac
    12361 gtactgacgg tgggagaatg ttaatccata ttggcagaac gaaaacgctg gttagcaccg
    12421 caggtgtaga gaaggcactt agcctggggg taactaaact ggtcgagcga tggatttccg
    12481 tctctggtgt agctgatgat ccgaataact acctgttttg ccgggtcaga aaaaatggtg
    12541 ttgccgcgcc atctgccacc agccagctat caactcgcgc cctggaaggg atttttgaag
    12601 caactcatcg attgatttac ggcgctaagg atgactctgg tcagagatac ctggcctggt
    12661 ctggacacag tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga gtttcaatac
    12721 cggagatcat gcaagctggt ggctggacca atgtaaatat tgtcatgaac tatatccgta
    12781 acctggatag tgaaacaggg gcaatggtgc gcctgctgga agatggcgat ctcgagccat
    12841 ctgctggaga catgagagct gccaaccttt ggccaagccc gctcatgatc aaacgctcta
    12901 agaagaacag cctggccttg tccctgacgg ccgaccagat ggtcagtgcc ttgttggatg
    12961 ctgagccccc catactctat tccgagtatg atcctaccag acccttcagt gaagcttcga
    13021 tgatgggctt actgaccaac ctggcagaca gggagctggt tcacatgatc aactgggcga
    13081 agagggtgcc aggctttgtg gatttgaccc tccatgatca ggtccacctt ctagaatgtg
    13141 cctggctaga gatcctgatg attggtctcg tctggcgctc catggagcac ccagtgaagc
    13201 tactgtttgc tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg
    13261 tggagatctt cgacatgctg ctggctacat catctcggtt ccgcatgatg aatctgcagg
    13321 gagaggagtt tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg tacacatttc
    13381 tgtccagcac cctgaagtct ctggaagaga aggaccatat ccaccgagtc ctggacaaga
    13441 tcacagacac tttgatccac ctgatggcca aggcaggcct gaccctgcag cagcagcacc
    13501 agcggctggc ccagctcctc ctcatcctct cccacatcag gcacatgagt aacaaaggca
    13561 tggagcatct gtacagcatg aagtgcaaga acgtggtgcc cctctatgac ctgctgctgg
    13621 aggcggcgga cgcccaccgc ctacatgcgc ccactagccg tggaggggca tccgtggagg
    13681 agacggacca aagccacttg gccactgcgg gctctacttc atcgcattcc ttgcaaaagt
    13741 attacatcac gggggaggca gagggtttcc ctgccacagc ttgatgaaga tctgagctct
    13801 taattaatga tcataatcag ccatatcaca tctgtagagg ttttacttgc tttaaaaaac
    13861 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
    13921 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    13981 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    14041 gtctggatcc gtttaaacta actttaaata attggcatta tttaaagtta catatggcgg
    14101 ccgccctaag gctcgacctc gaaattctac cgggtagggg aggcgctttt cccaaggcag
    14161 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
    14221 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
    14281 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
    14341 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
    14401 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
    14461 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
    14521 ctcaggggcg gggcgggcgc ccgaaggtct tccggaggcc cggcattctg cacgcttcaa
    14581 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcatc
    14641 catctagatc tcgagcagct gaagcttacc atgaccgagt acaagcccac ggtgcgcctc
    14701 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
    14761 cccgccacgc gccacaccgt cgatccagat cgccacatcg agcgggtcac cgagctgcaa
    14821 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
    14881 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
    14941 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
    15001 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
    15061 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
    15121 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
    15181 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
    15241 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc
    15301 gaaaggagcg cacgacccca tgcatcgatg atatcagatc cccgggatgc agaaattgat
    15361 gatctattaa acaataaaga tgtccactaa aatggaagtt tttcctgtca tactttgtta
    15421 agaagggtga gaacagagta cctacatttt gaatggaagg attggagcta cgggggtggg
    15481 ggtggggtgg gattagataa atgcntgctc tttactgaag gctctttact attgctttat
    15541 gataatgttt catagttgga tatcataatt taaacaagca aaaccaaatt aagggccagc
    15601 tcattcctcc cactcatgat ctatagatct atagatctct cgtgggatca ttgtttttct
    15661 cttgattccc actttgtggt tctaagtact gtggtttcca aatgtgtcag tttcatagcc
    15721 tgaagaacga gatcagcagc ctctgttcca catacacttc attctcagta ttgttttgcc
    15781 aagttctaat tccatcagaa gctggtcgag taactttaaa taattggcat tatttaaagt
    15841 taggcgcgcc ataacttcgt atagcataca ttatacgaag ttatgtcgag atatttaaat
    15901 attagaccca gctttcttgt acaaagtggt tgatatctct atagtcgcag taggcggtaa
    15961 ggtggtaggg tggggtggag aggagacagg gttccagcaa gagcaaactt cagttgctaa
    16021 ggatctattg gcagccgatc tgactgcttc tggcagagga agttttcttt aagggtacag
    16081 cccctggtgg gttggccgca ctctggtaga tggccccaca cccaggaata cacaggcaca
    16141 agttggactc agtgggaggg gtggagggtt ggaggtacag ctggaagggg ccagggagag
    16201 aagttagggt acatataatc aaaatacgtt ttaagtatgt atgaaattct caaatcaata
    16261 aaaaaattac aataaactat gtcagcctag accagacagt gcccaaggtc ccctggatcc
    16321 tggtcaccat ttctcaaggg ctcacaccta acttgctctc tggtccatgt gtcccacagg
    16381 tattgtgttc cactacagag ctatttccac acgctacacc ctggactttg atcgagcaca
    16441 gcgggcttgc ctacagaaca gcgccatcat cgccacccca gaacaactgc aggctgccta
    16501 tgaggatggc ttccaccagt gcgatgcagg ctggctggct gaccagacag tcaggtgaga
    16561 ctccgacccc cagaggaaaa tctgtcaggg ctgagagcca agtatcagag gcctagcaga
    16621 atgaggcaga cgatggggtg ggacaatgac ctgcactgag ctggtacaaa cataattctc
    16681 tggccagtct agggtccggc cttgtcaaca tgcatacgag atggcgcaac gcaattaatg
    16741 ataacttcgt atagcataca ttatacgaag ttatggtctg agctcgccat cagttcactg
    16801 tttttcctcc tgatgtttct ttgcttctgg tttgtttgtc agcttttaac ttggagtttt
    16861 atttttattt attgcctttt tattaatatt tatatagcct gttcatatat cataatatat
    16921 tacaacgata tattatatat tgcatagtat atataaatat attatgtgta cactgcctgc
    16981 ttatttatat cctttttttg taagacaaga catacataca tcaagtgtct agaaacatct
    17041 tttctgagtg aatcctagga gccacatggc aaagcagaac agaggactgc ctgaagcatc
    17101 tcagtacagt gtatcaagcc tgtgaaggca gacggtccac cccatataca tcctaaggct
    17161 agccccaaag ccagcccaca aggcctcagg aagacagcta tacaagctgg attagtctag
    17221 gggattttaa gtcacaagtt ctcacagcac tgttacagtt tcaactctgc tgctgagatt
    17281 aaacactgat caaaagcaag ttggggagga aacaatttat tccagcttct tccaggtcac
    17341 cgtccatcac tgaggtaagt cagggtagaa acacttgcag ttaggaacca tggaagaatg
    17401 ctgctttgtc aggcttacac acaggcttac acacaagctc acattcaatt agctttctca
    17461 cacatcccag gcccacctgt ctagggatag tgtcttccac ggtaggctgg gctctcctac
    17521 attgatcaaa gctcaagacc tcacagacct ggtcacaagc taatctgagc aatccctcag
    17581 ttgagacccc ttcggaagac tctagactgt gtcaagttaa cagctgacgc taactgggac
    17641 tggccctttc tacaaaaatt agtaaacaaa caaaagataa gtaagacaac agtctaacat
    17701 ggcaagagtc cacatattgg cctcaggaag agctagactc aagtcccaac cctggaattt
    17761 ggaagctgtg tgtctgttac caaatccgtt cacatttctg ggccttagtt taagtgtcaa
    17821 gagaatccag cccctgcttc actttagtag acctaagatt caagtggtac caagttggca
    17881 ctatggccaa atccaaacca accctttctt agtttctgta aatattgttg tgctgaaact
    17941 tagccatgct cactcggtta cacattgtct gtagctgctc cgtgctgctg cagtacagat
    18001 gatcaccaca gtggcccatg gtttgcaaaa cccacaagat tctgaccttt tacgtggaat
    18061 gtatctctgg gggagagagt gtttggtacc aagagcactt ggtagcagag gcatcgagac
    18121 tcatgagcag cattgagact gaaggagcag caggcatgca gagcagactt ggacagaagt
    18181 agttttatgc ctctgtctct tcctatggag gtgatggcta caaggacagt gactttgggg
    18241 taccttctgt tcctagtgtc acactcccac attgacttcc caacagatac cccatccaca
    18301 cgccccggga aggttgctat ggtgacaagg acgagttccc tggagtgaga acctacggaa
    18361 tccgggacac caatgagacc tatgatgtgt actgcttcgc tgaggagatg gagggtgaga
    18421 aagccggttc cctgcgagat gccttttcca tccccatcac cacgatcaca tgtgggatag
    18481 ctacacgagt cctcctctgg accaccaccc tccctcagtc ctctcctgcc atgagcagca
    18541 atggtctctt gctggaatcc atgctattca ctttgaaggg gttctcagtg gccacaggca
    18601 tctgtaaaac aatttagtgc taccactaaa ccccaagttc caaagtcccc tgggactgcc
    18661 cctcttatcc agcgtctcag catctctggc caaccccttt ctgccttaag gtccacagtg
    18721 ccatcaaagc catgctcagg tctagggtgt agctcagtgg cagaccctgg gctccatccc
    18781 tacgccactc taggtcagac acaacagaca cacaaacata cttcccagct accctctctc
    18841 catttcttgg cccccagctt gaagtcctac cctaggcctt ttgttgggtt gttcgaggga
    18901 gaaacctagg tagtttcttt cttttacgag ggtactaatt ttgaacatag agttaccctg
    18961 gcctgtagat gacacccctt tctcatctac ctctctggaa atcccacaca ctctgaatat
    19021 ccatctcata gctaccaagc tcctccagct gggagaaaag ctgaatggtg atatacctca
    19081 cctttcttcc cacgtgtccc catgtttgac taacccaagg caattaacag tccttttgtc
    19141 tgagcagcca ctgagcaagg acacctgaaa ctacccaata actgatcaga gactgcccac
    19201 aaccccaccc catcctcaaa cctttccaag tttaggaaat attgtttaaa agctagcaga
    19261 ccccaaatta ccctataatg ggctctttgg gtactactgg ccctggtttg gaaggcagta
    19321 ctctaaggga tcaaaaggcg aggccagctt ttctcatcag attttcaatt tgggatagtt
    19381 tcctaggaat ggatagtgcc tgtgttaaag gaaagctccc ctagtggtgg gagggtgaat
    19441 agcaaccaac tgggcttctg atttcgggat tgctcgaccc attgcatttt gggtagcttt
    19501 ttttgggggg ggaggggggg gagaaggtcc ttgttaggac attggtgtca tcctacctaa
    19561 aagttctgag aggaatgaag agtgtggtac taacgcagaa ccaaggatag caagctaaac
    19621 ctccgggaaa agatgtggac ctctcaggga cacaggcagc ttgggatgtg agcgaaaccc
    19681 tcacccagtg tgatgcgtct gtccttcaca ggtgaggtct tttatgcgac atccccagag
    19741 aaattcacct tccaggaggc agccaacgag tgccggaggc tgggggcacg cttggccacc
    19801 acaggccagc tctacctggc ctggcagggc ggtatggaca tgtgcagcgc tggctggctg
    19861 gcggaccgca gcgtgcgcta ccccatctcc aaggctcggc ccaactgcgg gggcaacctc
    19921 ctgggtgtaa ggactgtcta tctacacgcc aaccagacag gctatcctga tccctcatcc
    19981 cgatatgacg ccatctgtta cacaggtggg actggggctg aaggtgggaa gggggggttc
    20041 tcgccaccga tatcagatcc cagtaagatc ttgggaaggg atcactgagt ttctgaactt
    20101 gggttttcac tgcttcttaa tgtagagaca atggagtcac aatgtagcca gcccagtttc
    20161 atcagatcca cacagaacag tgtttaaagt atatgcttcg gagccactgt tttctagggt
    20221 ttgaattcca gctcttccat accacaatgg ctcatttcat atttcacttc tacagcccaa
    20281 tcttatgtga agagggacac cctctctccc aaaagcctct cttctctttt cttgataatc
    20341 tgaccactct ttggaaatgt caccctcatt aatattcttt tttattaata actaccttat
    20401 gtctgccagt ccctcatttg tccaggactc tgagacaggc agttctccag caatgcctgc
    20461 atcaattaca caaagtatgt ctgcaagatt ccggcttccc cttcctcaca ctgtcccatt
    20521 ggacactgag cagacaaagt agccgctggt ggggaggcgg aaataggaac tgattttatt
    20581 tgataagcaa tgagctagct gatcaatata ccaggagtag cctagtgctt ctacaaattc
    20641 ggccaacctc agactcctat taataattta attgcatcta tactgaatag gtgcagaatt
    20701 tgtcttgcca tcattacgca actgt
//