LOCUS       KO-first_condition_ready_121522_MGI:88453        22217 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Col3a1 targeting project(s): 121522
ACCESSION   unknown
SOURCE      Mus musculus.
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 209370
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..10279
                     /note="5 arm"
     misc_feature    10307..10331
                     /note="B1 Gateway"
     misc_feature    10344..10391
                     /note="Frt"
     misc_feature    10392..10891
                     /note="mouse En2 intron"
     primer          complement(10460..10481)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10356..10379)
                     /note="FRT3"
     primer          14557..14575
                     /note="seq primer NF"
     misc_feature    14617..14624
                     /note="RE sites"
     misc_feature    16417..16450
                     /note="loxP"
     misc_feature    complement(14625..14656)
                     /note="Rox"
     misc_feature    complement(16377..16408)
                     /note="Rox"
     misc_feature    14657..14678
                     /note="polylinker"
     CDS             15237..15833
                     /note="puromycin N-acetyl transferase"
     misc_feature    15352..15357
                     /note="Mutated RsrII site"
     intron          10892..11266
                     /note="mouse En2 intron"
     misc_feature    11264..11266
                     /note="Splice Acceptor"
     exon            11267..11320
                     /note="mouse En2 exon"
     primer          11167..11188
                     /note="seq primer FCHK2"
     misc_feature    11321..11443
                     /note="1Dleader"
     misc_feature    11444..11495
                     /note="F2A"
     misc_feature    11591..12307
                     /note="EGFP"
     misc_feature    11501..11590
                     /note="3X NLS"
     misc_feature    14373..14611
                     /note="SV40 polyadenylation site"
     misc_feature    13406..14350
                     /note="ERT2"
     promoter        14689..15200
                     /note="PGK promoter"
     misc_feature    15944..16376
                     /note="PGK pA"
     primer          14431..14454
                     /note="LRPCR RAF5"
     misc_feature    12317..12370
                     /note="T2A"
     misc_feature    12367..12367
                     /note="A to G to kill RsRII site"
     misc_feature    12371..13396
                     /note="Cre"
     misc_feature    11282..11321
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    11282..11297
                     /note="FCHK QC prefix region"
     misc_feature    11298..11315
                     /note="FCHK QC critical region"
     misc_feature    11316..11321
                     /note="FCHK QC suffix region"
     misc_feature    16473..16493
                     /note="B2 Gateway"
     misc_feature    10280..16523
                     /note="Synthetic Cassette"
     exon            16892..17091
                     /db_xref="ENSEMBL:ENSMUSE00001005682"
                     /type="targeted"
                     /note="target exon 1 ENSMUSE00001005682"
     exon            17337..17387
                     /db_xref="ENSEMBL:ENSMUSE00001041957"
                     /type="targeted"
                     /note="target exon 2 ENSMUSE00001041957"
     exon            17801..17914
                     /db_xref="ENSEMBL:ENSMUSE00000700263"
                     /type="targeted"
                     /note="target exon 3 ENSMUSE00000700263"
     misc_feature    16524..18035
                     /note="Critical Region"
     misc_feature    18059..18092
                     /note="Downstream LoxP"
     misc_feature    18036..18115
                     /note="synthetic loxP region"
     exon            19166..19240
                     /db_xref="ENSEMBL:ENSMUSE00000700261"
                     /note="ENSMUSE00000700261"
     exon            20803..20856
                     /db_xref="ENSEMBL:ENSMUSE00000700256"
                     /note="ENSMUSE00000700256"
     exon            21248..21301
                     /db_xref="ENSEMBL:ENSMUSE00000700252"
                     /note="ENSMUSE00000700252"
     exon            22014..22067
                     /db_xref="ENSEMBL:ENSMUSE00000700249"
                     /note="ENSMUSE00000700249"
     misc_feature    18116..22217
                     /note="3 arm"
BASE COUNT     6430 a   4851 c   4686 g   6249 t     1 others
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatccgaa ttactaatca catagaacca atggaataaa atgtattaca
     4321 aagtcacggt agtaacctta cttctaacag aagaaaatac acaattatgt acatatttaa
     4381 atatcagtca tgtaacctac agtgtgtttg taaagcttaa aaacaggaat ggatgaatag
     4441 tttagtactg aaactaaaat ttaatgaaat actacatgat ttgagaacaa aagcataaac
     4501 cttgaacatc tgcctttttt tacttttgta agtatttgta aataatagag attccaagtt
     4561 ccataccatt tttgaaaatt tatttataaa gttttttttt aatggtagtc cttttttcta
     4621 ttccttactc aatttttaac aaattaatct ggtttattaa gcaaaaggct caggagctaa
     4681 tgacttaacc agaaattatc attctttctg taaaaatgta cattgtaagt aaataaagta
     4741 ttaccaatac aagattttgt tgtttctatt cataaccccc ttttatttcc tcagcatctc
     4801 aacagtactc agaatgatgg gtaaagggcc tataacagac cctgcatcac atttagatct
     4861 aggatggacc agtcaagcat tgtagaagag agcaagtctt cggaaaccat gctgggaggg
     4921 agaagtggag aggaaaggcc cattaatgag tgcagattct tcggtgactt gcctttggat
     4981 ttctttttta aaagatttgt ccagaactat aggagatgct aaaagaaagc aaggcctcat
     5041 tagaaaggcc tgtttcatat ctacaggctc agaccaggcc tggagtggta actcagaaaa
     5101 gactgccaac cactgaagct ctgtggtgac cactgaggat gactgtccct agtgccgggt
     5161 tcctatgtgt actttgtgac aggcaataac tgataacgcc accttgaagc cattaaagtg
     5221 attgcttgta agtcagagcc gggaagatca aagatgagct tgcctttgct ggacctggca
     5281 gacactaaag cttcgggtac actgtgactt tctggcaatg tgtgtgagct aattctcata
     5341 aaatgtttcg ttctgtttct tagagctaaa agcttagtgt cattattttc catgcttagg
     5401 aaaaaaaaaa atccacattg tttttttttt taatccaaaa gttttaaagg aggaaacttt
     5461 gcctgtcagg aaagcagtgg aaactctgag ctagctctgc acagcagtcg tttgttagct
     5521 gcactttgga gagttacatt aaccactaga agttctgtct gtgcctaaca aaaacatagc
     5581 ccaagtggat tcctttcaga ttaactaatc aaagccttgc tttttccatg gctgaggtca
     5641 agccctgcca tttcaaatgt aatctcaatg aacaaaacgg aaagttcaaa tggaagaaat
     5701 actaaaattg ccccaaagaa gaaattgcca caactaatcc aaaacttatt aatagttaaa
     5761 ttagctttga taattagcat tgaaggatga ctttctcagt taacatagtt gtgagacatt
     5821 ttttgttctt atgtttgtta ttgtagaggc caacaagcta tgtttctcac agaaacactt
     5881 tatgatgttg cacagaattt atagtgccca agtagcattc aaaggagact tgtgctcaag
     5941 ttagaagtta acaaatgcaa atcaagcaaa aactatagtt tttgaatagt agtgccttag
     6001 ccatcctaaa tacatctatg tatctgatga agaatgttta tgtttgtttt tgtcagtctt
     6061 agtgcctggc ttccaagtca cacattcatc ttgctggtgg tctggatccc atcagggtgg
     6121 ccttggaaat acaagccttt ttcataaggt caagagaaat aaacagtagg attccactcc
     6181 agatggtatc ttcccatttt aagattagag aaaaaaaaga cattttggag atcactgtta
     6241 ttccttaaaa aaaaaaaaat ctgtctgcat actcgagagg gtacagagtt cacaaaccat
     6301 ctgggacagg gcactgaaac aggtctcttt cattctatag tatccttgaa agcagtaaaa
     6361 acataatgga gttaggagta tcagatttta gaaaagcata ctagattcaa atctgaattc
     6421 ctttaagtat tttacctgga taattactca gtttgaccca gattctttgt gtgcattcag
     6481 gattgtgaca gcattcagct caaaacatgc aggaaggaga tcataaaatt gatataaacc
     6541 atcaggtata gtgatgaaca cctgcattcc cagcagtcga atgcagtgat acacaacggc
     6601 atttcgagca gtttgatatt agtgatgcat acctgcattg ctagccgtct gcacctatgt
     6661 tcccagcaat ctgaaacctt ggagcaagga ctgtggtttc aaggctagcc tggtggcatc
     6721 tataatttga aagaaaagaa aaagatgtca gccactcaag agtagtacct agaacagagg
     6781 cttgatctat cattcgtttg ccaagtactg tcaactacca tgacaccgtg aaaagaatta
     6841 aagtatcatg taatgatagg aaagattaca tgaacatctg cattctcaaa gtataaaact
     6901 aaagttgcta cacagtaaat taaatatatt aaatattttc ataatttggt aggatccatt
     6961 atagatgtat ttcgatgatt atacatactt tacttttcca tttatctatt tttattttgg
     7021 caaaataaat atcaatcaag ttgtaggaaa agtttaggga cataaaaagc atgtgcctat
     7081 acagaataaa acaaagaaac acctaagcat cattcattta gttttcttgg tggaaagtag
     7141 aataaccaga atctacctag ccatgtaaaa caacacttaa aaatgcactt catttctatt
     7201 tcaaaagact gtctcttcag catctctgtg ctgatgctag aaaggtggct caggagtgca
     7261 gagtgcgtcc tgcacttgca gaggtcccaa ggtcagcttt gagcatctta ctttattacg
     7321 ttgtccagaa ttgaaaatgc aattgtattt atttaatatt tcttacaaaa gtattcagct
     7381 tcctctttat caaagtttct gtgaacacag caccctaata agtgaaaggg acactgtgag
     7441 gtaacttagt cccgcatggc aggtgttatc ctatctttaa atgctgcaaa gcatagagca
     7501 ttccaggaaa tgtcctcttt ccctgcttta caataacttc ccaagttaga tactgaagct
     7561 ggagtcatga ggtacatata tcataggctc agcttctggt atcctacatt ggtaacagta
     7621 gattgccttg tatgaggtga tgaatcagaa gaagttgtgc aacgccccct ttttaggcaa
     7681 agatgactaa gataaatagg aagtgctagc tttcagatgc aagatctact tgtgactttt
     7741 cagaatctga tgtttcaagc atgccatctt tgtttcaagg ggacatgaag taagtctggg
     7801 caacaaatta gattcattat ttcttatttt ttagacattc caagtttatt tatgtagaac
     7861 aacacaactt tctaaagcaa aagaccatat gcaatatcat tgtaaatgtt ctgagcagat
     7921 taaaagaaag aactcacccc aaaaattgag gttacattag gattacaatg gtggtctcta
     7981 gaggcaaagg gcaggagaag aatcgaggat tgcgcagaat gggtgccaat gagaattgac
     8041 cattagataa caccaaaaat attctggtgt agaactacac agtaggacaa cttctagcaa
     8101 taaaagtgtg tagtgtattt taaaggctag gtggaagtat tttcaaaggt tttaccataa
     8161 attacaaatt tttgaggaga taaatatgct taatctggat taaacataat attggtttat
     8221 atgtgtcaat ataatacatg agactccatt aaggtgtagg gtttttacaa cttgatatat
     8281 taattaaagt gaagtaaaaa tgtttttaaa atataagaag taaagatgta ctaagatcca
     8341 ctgaccatta tgttgaaatc tctacacttt aaaaaccact caataagatt tctaagaaaa
     8401 acttagaaac aggaagccaa ataacagttt tctatcatgt aaccctgggt ttaacatatt
     8461 catttaagct tttaatgagt cagcgtaaac attccaaaca aataacaaac actgtctttc
     8521 ttctgatgtg agaatacctt caatgaggta tacacttgtg aagaagaatg catgagaaaa
     8581 acgtttcatg gtatttctaa ggtcatataa aatatatgat gttttttaaa atatgaggaa
     8641 tttaaatcta ttttaattat attttaaata aaataataat tttaataatt attataatga
     8701 aataataaaa tgattttaaa tttattttaa tgtattttat tgtagtttta ctttttaaag
     8761 tatccattag ttccttttaa taacctgcac actttattaa atgtggcaaa atatttctca
     8821 tgcaaaaata aatttttgaa agaatcatta gctcaattta ctagtatttt aagagttgag
     8881 atcaggcagt ttgatgtcca catgttcatg tggaatatcc taaattgaag ctaatcaaat
     8941 gagtatgtgt cagtctaata cagagcatca ttttttattc acattaaaga aggaggaccc
     9001 atgagaagat tttagtgttt tcactgataa tcttttattt cagcacttta atttcatttt
     9061 ttcttaaata caaaatatca gttctaggta aatatacatg gggttaggac ttagcttttt
     9121 tctttaaaac agcaccccag acagccctgc atcaattact ctgaggtatt cagtttggta
     9181 tttagctttt acaatcatta agaatgaagt tgtggtcttt aaagccttag gaatatctgt
     9241 cctgttctca cagtctgtta cagacatcag aggaggatct aagacaactt tgctatctaa
     9301 cacagtttgg ttagtttgtc ttggtttggt ttggtttggt ttggtttggt ttggtttggt
     9361 ttggtttggt ttggtttgac tgtattgtct ttgttactta ttgaaaatgt ctaaactgtc
     9421 tttgactggc tgttaaatca gtacattgtt aagataaaaa ataaatctta cttcatacaa
     9481 gaagcttgag ctatttaatg acatgattaa agtagtattg gcatacatac ataaaagaga
     9541 attagaagca actctgtagt ctatacagac tggatgttct gaaatttgtc ttattgtatt
     9601 taagatttag attattaatg gttttagatt actcaaagta tctaaatatg aagataggga
     9661 attagaaatt ttaaattata attgggctta tgagaaaata aagttttact ctctgagaaa
     9721 atgtaaaaga actcagaaag gcattgctta tattcaaaat tatttgcaga tgtttgaaaa
     9781 cttattattt attcattcat tattggcaaa ctctcatagc aattttcaag ctgcatttaa
     9841 ccatactaca cagttaacat ttcaagaatt caggccaggt tttattttgg catgacttaa
     9901 gttgattcac tgtaaatgac tgctgaaatt tcaatttctt atatataatt aagaaccaag
     9961 tctaaaattt tggttgaagg tatttctctt gccatagtaa gccacaaata cacaaaccat
    10021 cattcgtact tcccctatta tttttactta ttcattgtca atatttgttt taaagtatca
    10081 ctttattttg gggcatttgg aaaactcttt tgttcttgta tgatatacaa cacattatgt
    10141 gatataatac tgacccacta atgtccaaca gagtgagcag tgacattcaa cacaaatcag
    10201 ctttgttagt tctcccagga caatatggaa gaggtttata ctgccaagat tagctcaatt
    10261 tttccatgga tttttgaaga aggcgcataa cgataccacg atatcaacaa gtttgtacaa
    10321 aaaagcaggc tggcgccgga accgaagttc ctattccgaa gttcctattc tctagaaagt
    10381 ataggaactt cgaacccttt cccacaccac cctccacact tgccccaaac actgccaact
    10441 atgtaggagg aaggggttgg gactaacaga agaacccgtt gtggggaagc tgttgggagg
    10501 gtcactttat gttcttgccc aaggtcagtt gggtggcctg cttctgatga ggtggtccca
    10561 aggtctgggg tagaaggtga gagggacagg ccaccaaggt cagccccccc cccctatccc
    10621 ataggagcca ggtccctctc ctggacagga agactgaagg ggagatgcca gagactcagt
    10681 gaagcctggg gtaccctatt ggagtccttc aaggaaacaa acttggcctc accaggcctc
    10741 agccttggct cctcctggga actctactgc ccttgggatc cccttgtagt tgtgggttac
    10801 ataggaaggg ggacgggatt ccccttgact ggctagccta ctcttttctt cagtcttctc
    10861 catctcctct cacctgtctc tcgacccttt ccctaggata gacttggaaa aagataaggg
    10921 gagaaaacaa atgcaaacga ggccagaaag attttggctg ggcattcctt ccgctagctt
    10981 ttattgggat cccctagttt gtgataggcc ttttagctac atctgccaat ccatctcatt
    11041 ttcacacaca cacacaccac tttccttctg gtcagtgggc acatgtccag cctcaagttt
    11101 atatcaccac ccccaatgcc caacacttgt atggccttgg gcgggtcatc ccccccccca
    11161 cccccagtat ctgcaacctc aagctagctt gggtgcgttg gttgtggata agtagctaga
    11221 ctccagcaac cagtaacctc tgccctttct cctccatgac aaccaggctc ccaggtcccg
    11281 aaaaccaaag aagaagaacg cagatcgcag atcgcagatc tctagagtca ccgagttgct
    11341 ttaccggatg aagagggccg aaacatactg tccaaggccc ttgctggcaa tccacccaac
    11401 tgaagccaga cacaaacaga aaattgtggc accggtgaaa cagactttga attttgacct
    11461 tctcaagttg gcgggagacg tcgagtccaa ccccggaccg gtgcatgtgg accccaagaa
    11521 aaagcggaag gtggacccta aaaagaaacg caaagtggat cctaagaaaa aaagaaaggt
    11581 gcacgtgtcc atggtgtcca agggcgagga actgttcacc ggcgtggtgc ccatcctggt
    11641 ggaactggat ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgaaggcga
    11701 cgccacatac ggaaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
    11761 ttggcctacc ctcgtgacca cactgaccta cggcgtgcag tgcttcagca gataccccga
    11821 ccatatgaag cagcacgact tcttcaagag cgccatgccc gagggctacg tgcaggaaag
    11881 aaccatcttc tttaaggacg acggcaacta caagaccagg gccgaagtga agttcgaggg
    11941 cgacaccctc gtgaacagaa tcgagctgaa gggcatcgac ttcaaagagg acggcaacat
    12001 cctgggccac aagctggagt acaactacaa cagccacaac gtgtacatca tggccgacaa
    12061 gcagaaaaac ggcatcaaag tgaacttcaa gatccggcac aacatcgagg acggctccgt
    12121 gcagctggcc gaccactacc agcagaacac ccctatcggc gacggccctg tgctgctgcc
    12181 tgacaaccac tacctgagca cccagagcgc cctgagcaag gaccccaacg agaagaggga
    12241 ccacatggtg ctgctggaat tcgtgaccgc cgctggcatc accctgggca tggacgagct
    12301 gtacaagcct gcaggcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
    12361 tcccgggccg tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc
    12421 aacgagtgat gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc
    12481 tgagcatacc tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt
    12541 gaataaccgg aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct
    12601 tcaggcgcgc ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct
    12661 tcatcgtcgg tctgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg
    12721 gcggatccga aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga
    12781 acgcactgat ttcgaccagg ttcgttcact catggaaaat agcgaccgct gccaggatat
    12841 aaggaatctg gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc
    12901 caggatcagg gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg
    12961 cagaacgaaa acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac
    13021 taaactggtc gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct
    13081 gttttgccgg gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac
    13141 tcgcgccctg gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga
    13201 ctctggtcag agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga
    13261 tatggcccgc gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt
    13321 aaatattgtc atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct
    13381 gctggaagat ggcgatctcg agccatctgc tggagacatg agagctgcca acctttggcc
    13441 aagcccgctc atgatcaaac gctctaagaa gaacagcctg gccttgtccc tgacggccga
    13501 ccagatggtc agtgccttgt tggatgctga gccccccata ctctattccg agtatgatcc
    13561 taccagaccc ttcagtgaag cttcgatgat gggcttactg accaacctgg cagacaggga
    13621 gctggttcac atgatcaact gggcgaagag ggtgccaggc tttgtggatt tgaccctcca
    13681 tgatcaggtc caccttctag aatgtgcctg gctagagatc ctgatgattg gtctcgtctg
    13741 gcgctccatg gagcacccag tgaagctact gtttgctcct aacttgctct tggacaggaa
    13801 ccagggaaaa tgtgtagagg gcatggtgga gatcttcgac atgctgctgg ctacatcatc
    13861 tcggttccgc atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat ctattatttt
    13921 gcttaattct ggagtgtaca catttctgtc cagcaccctg aagtctctgg aagagaagga
    13981 ccatatccac cgagtcctgg acaagatcac agacactttg atccacctga tggccaaggc
    14041 aggcctgacc ctgcagcagc agcaccagcg gctggcccag ctcctcctca tcctctccca
    14101 catcaggcac atgagtaaca aaggcatgga gcatctgtac agcatgaagt gcaagaacgt
    14161 ggtgcccctc tatgacctgc tgctggaggc ggcggacgcc caccgcctac atgcgcccac
    14221 tagccgtgga ggggcatccg tggaggagac ggaccaaagc cacttggcca ctgcgggctc
    14281 tacttcatcg cattccttgc aaaagtatta catcacgggg gaggcagagg gtttccctgc
    14341 cacagcttga tgaagatctg agctcttaat taatgatcat aatcagccat atcacatctg
    14401 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
    14461 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
    14521 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    14581 ccaaactcat caatgtatct tatcatgtct ggatccgttt aaactaactt taaataattg
    14641 gcattattta aagttacata tggcggccgc cctaaggctc gacctcgaaa ttctaccggg
    14701 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
    14761 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
    14821 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
    14881 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
    14941 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
    15001 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
    15061 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcttccg
    15121 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
    15181 catctccggg cctttcgacc tgcatccatc tagatctcga gcagctgaag cttaccatga
    15241 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
    15301 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccagatcgcc
    15361 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
    15421 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
    15481 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
    15541 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
    15601 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
    15661 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
    15721 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
    15781 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
    15841 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgatgatat
    15901 cagatccccg ggatgcagaa attgatgatc tattaaacaa taaagatgtc cactaaaatg
    15961 gaagtttttc ctgtcatact ttgttaagaa gggtgagaac agagtaccta cattttgaat
    16021 ggaaggattg gagctacggg ggtgggggtg gggtgggatt agataaatgc ntgctcttta
    16081 ctgaaggctc tttactattg ctttatgata atgtttcata gttggatatc ataatttaaa
    16141 caagcaaaac caaattaagg gccagctcat tcctcccact catgatctat agatctatag
    16201 atctctcgtg ggatcattgt ttttctcttg attcccactt tgtggttcta agtactgtgg
    16261 tttccaaatg tgtcagtttc atagcctgaa gaacgagatc agcagcctct gttccacata
    16321 cacttcattc tcagtattgt tttgccaagt tctaattcca tcagaagctg gtcgagtaac
    16381 tttaaataat tggcattatt taaagttagg cgcgccataa cttcgtatag catacattat
    16441 acgaagttat gtcgagatat ttaaatatta gacccagctt tcttgtacaa agtggttgat
    16501 atctctatag tcgcagtagg cggcccatat gttattagaa catagaagag caattgggta
    16561 aaactgttca tttccagctg cataagtaat agacaagagt ttgaatgtta tctactggtg
    16621 ttaagctttt taatgtaaat gaagtaaaaa caattgattt gatgaaaaat tgcactgtgc
    16681 caattttatg ataacaagta gttccttggt acatagaaaa tatagagggg aaacattatc
    16741 tattctgaat gaaagaagag catttcttgt tccaaacttt cttcacttta actctttttg
    16801 taaatagtca ttactgcaca accaaaacca aatgtacatt ttgtcaaaac atttcttaaa
    16861 ttgccatatc ttctcattta tcttcccaca gatgaattgg gatgcagcca ccttggtcag
    16921 tcctatgagt ctagagatgt ctggaagcca gaaccatgtc aaatatgtgt ctgtgactca
    16981 ggatctgtcc tttgcgatga cataatctgt gatgaggagc cactagactg ccccaaccca
    17041 gagatcccat ttggagaatg ttgtgcaata tgcccacagc cttctacacc tgtgagtctg
    17101 tagaatatat aaactcttca aaatctacat cttcaagaac catatttaaa catgtaaata
    17161 gtctctactg catagaggtc tgaaagaaat gcaagccatg tctgttgcca tgacaagtga
    17221 atggtgtcac ttttaacaga aagcaggaga ttgggtataa tcagatttaa gaaataaaga
    17281 tgtaaattgt gctacagttt acatatatta atctctatat tctttatatg ttataggctc
    17341 ctgtgcttcc tgatggccat ggacctcaag gccccaaggg agatccggta agtaacatct
    17401 ccccaaggac aatagttgct aacgacattt ctagaaaagg ctggttttcc ttgttccaac
    17461 tgaattttca tcattctgaa ttttcttctg tcctaagtat atgaaaatga tggcaaactc
    17521 atactgaatg tctataactg atcacatatg taaaggttca gttttcccaa gtttgaaagt
    17581 cttcaaccct ttttccattt taaagagaaa atgtaggtca tgatataaag gaaaacgata
    17641 aaccattatt cataagactt agtttgaaat atacttatgc ctaaggacag attattgtac
    17701 tgataactag tttggtaaag catagaaaaa ttcctaataa tgattctgaa ccacttcatg
    17761 ttttctctgt tttgttgttt tgttttcctt gttttgttag ggtcctcctg gcattcctgg
    17821 gagaaatggt gaccctggcc ttccaggaca accaggtctc cctgggcctc ctggctcccc
    17881 tggaatctgt gaatcatgtc caactggtgg ccaagtaaga aattacagaa ttaaattctc
    17941 ttctatataa gtgcctgagt ttgtcattgt ttggtctata tttttatctc ataatggcaa
    18001 cttctgtcct ctagttttct atatttataa aacccgagat ggcgcaacgc aattaatgat
    18061 aacttcgtat agcatacatt atacgaagtt atggtctgag ctcgccatca gttcatattc
    18121 aactgtcatc atgtaataca attgctacta tatccttcta aagtttccca atttctcaac
    18181 atgtccacat tacactcact aggtttgata aacagactat tagctcctaa aatagttatc
    18241 ttagagtatt taggaagtgc gataattaca ggcaattccc taatcttatc caagcacaaa
    18301 tggcaaagac ttacaaggct gggcactaaa cagctgctcc aaaaactgcc tattgaattc
    18361 atataaacag agttgcccgt gcttctgtgc agcctttaat gcacatcttt ttcccttctg
    18421 atttagggca accaatcagt ctaggaataa aataaataac taagaacata aacattgtaa
    18481 agttctgctg tatgggttta cacgaactta taaaactgta caaaccaata tgtatgattt
    18541 ataaaaggta ttttccaaaa caattcagca gcaaggagca tcctagaaat caaatagagc
    18601 cgagctagcc ctagaactca aagcaagctc tgagcctttc tgatttgttt tattgtttac
    18661 aatgattcat gactgaagtt cagcttttat tttattaaat cataggcatg gcatatatat
    18721 aatatatata tatatataat tttattgact tcttgaaaaa taattttaat acccattttt
    18781 cgtcagatat tagaaacttg gattttgttg cttcaaagtt attttgtcct caaatattgg
    18841 aggattctgt gtatagtgct ataacaattg tgtagaaata atggtttcag taataacatt
    18901 tgagaccaaa ttgaagagga cagttttgtt taatatctaa agttaaaatc tcctccatac
    18961 attacacaag caagcatttc tatagtgtgt ctaatattta aagccataaa atgaaacaat
    19021 aagcaagata tacatatcat atatgacatg cagaaatgtc agtgaacagt acatgttaca
    19081 cacactaact acaaatgctt tggaaaataa ctatgatctt tgtattaatg tgatctgatc
    19141 tatgtactta acccttttgc tacagaatta ttctccccaa ttcgactcat atgatgtcaa
    19201 gtctggagtg ggaggaatgg gtggctatcc gggaccagct gtaagtacaa atgtttctga
    19261 gtgttttcaa gtatcacact gcctttgttt cttctagaaa tacaatgttg gcatgcaatt
    19321 gtacaaaaag attcattgac tactaaggtt ggaagagaaa acagcaacct gtccatgatt
    19381 tttctatcct aactcattcc ctccctgtgg cgttttatgt cattccatat tgctgcttcc
    19441 taatgcccac ctgtactagg gctttttcca ccaccatgct ctggtccgtt cttttttcat
    19501 ctaatgtgta actaaatgta gttttgttag ggattgcttc tactttacat cctctcaccc
    19561 aagttgcatc ttttttcatc ttgttcactg atttttacat tctaaatgaa gtttaagtca
    19621 tgaatgtcct atatcacagt ctgtttttca ttcgggtttt aaatccagca ttagcatctt
    19681 tagaatgcat tgatctttta tgttccacta ccataaacaa tacctcaacc ttgacagtaa
    19741 tgggctttca aaactttcta aatgaccgag aattttagta acagaaaaga gcaaaaagtt
    19801 tgaaaactgg atttgaagtc accgatgttg gtgacttttg ctttggaata gctctcagga
    19861 agtacaacaa tattgaaccc tataattcag agaaagatca gcataaataa atacagtata
    19921 gcatatattt gtcaaggtaa agaaaaaatt tttatcagta aatccaaagt acctacaaaa
    19981 gagtactgta tttggtataa aagaaattga aatatatatt tcttctacag tttttgatat
    20041 gatttttaaa actatttgaa aatcaaatgg agattatatt atatttgtaa atattggtta
    20101 ttattacact catgttataa atcctccctt aaaagattaa caaaaccaaa taaaactaca
    20161 atttattgac atttcagaga gataacctaa agttaagtta aaattttgtt atcagaatat
    20221 aaaaatagta taaatctgtt tttctagttt ggtgtgaatt tctgaataat gtctagataa
    20281 atttgtttta agttgtgagt gccccatgca taatgattca tgtttatatc taaatcttca
    20341 catcaacatt actgtggtat atcatgatag ctatgaggtt tggagttgac tgacatggtt
    20401 attgtagaaa cttataccat aatatctata gtatgaagat atttccacaa tatgcttatc
    20461 caaaaaaatt tataacctag agaggtatct atgaaatatt ataaagctta tcatcacatt
    20521 ttatactgac tttctctgaa ttccctttct tatgtgttag ggctaacata tttaagatta
    20581 attggtaatc tagactgtct ctctaatttc atctgaagta aagttttcat gcaaaaatct
    20641 ttattgaaaa tgtcccagaa ctaaatatgg tatgttatca gataacaaga tataaagttg
    20701 tatatgttat aatacatact ggcattatat gggaatagac actactctac aatgtggaca
    20761 tttatctact gggtttgttt atcatacaaa tttacattcc agggtccacc aggccctcca
    20821 ggaccccctg gttcttctgg acatcctgga tctcctgtaa gtatcattct ttttggtgct
    20881 ttcttcttca tgaatatatg aagcaagcta atgcagttat ggatataaat cagccatttc
    20941 agtatccata atcccaacca ataaataatt cactccccaa caattattag caattaaatc
    21001 tttggtaaaa ttaaaataca tattacatgt gtatacaaca gactttggag tccataacca
    21061 ctcaatttcg gtgaaataaa agaaaactaa ctttattgtg atcctgaaaa cctcttaaat
    21121 catcataaaa tataataaaa tttctgccta catccactaa ccatgacttc ttacaataat
    21181 ccagatgtga tttactcatg tgaagctctt gttacctaaa tatatttaca ctattcttct
    21241 attttagggt tctcctggat accaaggtcc ccctggtgaa cctggtcaag ctggtccggc
    21301 agtaagtaac acttgagaat tgctaaacac attgaaaatt catttcccac atgaattcta
    21361 tcatcagttt ctaagtcatt cattgttcat ctagagtgta gattgcacac atgcttgaat
    21421 tttccatgac aatatagctt tttttctgtg aggaacttga ggtgaactca aagactttag
    21481 agattcctga ctggttaacc accaaaaatc atccacttcc cagaaataac aaaatttcct
    21541 atctaggaaa tacatgtcta atgtttctgt agattcgttt tatatgaatt ctaccacaat
    21601 gcctacaaat atacatacat ctgcatagtg cagagagaaa gacaagaata tccaagaata
    21661 atatgccata taggtatcaa aaattaaaat aaatgttatt ttaagacaat attttgaaat
    21721 attgcaaaat gtttttatat ttttgtatag tcattatctc aaaatataga aatacaaata
    21781 taaatataca tatttagtat gacatagata catgatattt aaatatttca ctaaaattta
    21841 ttcaacacta aaacttaaat ataatgaagg tataatatta attcagaata attttcatgg
    21901 tgaggacaga gggatgtttc aaagacacat ttgcttccag aatgcaaata tcatacaaca
    21961 agaaaatgta acacaaaata ttgaatattt ctgtttctat tttcttattt cagggccctc
    22021 caggacctcc tggtgctctt ggtccagctg gtcctgctgg aaaggatgta agtacttata
    22081 agttaaatag aaatacagca acattcatgt tgaatgacag actgccttaa atgtatccac
    22141 ttttgaagaa tcttagtaat ggaagcctct ttcaaaaaaa ttgtacccaa caaacatttt
    22201 agctcatatt agaggtt
//