LOCUS       210262#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20931 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 210262
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000068584
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..10665
                     /note="5 arm"
     misc_feature    10693..10717
                     /note="B1 Gateway"
     misc_feature    10730..10777
                     /note="Frt"
     misc_feature    10778..11277
                     /note="mouse En2 intron"
     primer          complement(10846..10867)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10742..10765)
                     /note="FRT3"
     primer          14943..14961
                     /note="seq primer NF"
     misc_feature    15003..15010
                     /note="RE sites"
     misc_feature    16803..16836
                     /note="loxP"
     misc_feature    complement(15011..15042)
                     /note="Rox"
     misc_feature    complement(16763..16794)
                     /note="Rox"
     misc_feature    15043..15064
                     /note="polylinker"
     CDS             15623..16219
                     /note="puromycin N-acetyl transferase"
     misc_feature    15738..15743
                     /note="Mutated RsrII site"
     intron          11278..11652
                     /note="mouse En2 intron"
     misc_feature    11650..11652
                     /note="Splice Acceptor"
     exon            11653..11706
                     /note="mouse En2 exon"
     primer          11553..11574
                     /note="seq primer FCHK2"
     misc_feature    11707..11829
                     /note="1Dleader"
     misc_feature    11830..11881
                     /note="F2A"
     misc_feature    11977..12693
                     /note="EGFP"
     misc_feature    11887..11976
                     /note="3X NLS"
     misc_feature    14759..14997
                     /note="SV40 polyadenylation site"
     misc_feature    13792..14736
                     /note="ERT2"
     promoter        15075..15586
                     /note="PGK promoter"
     misc_feature    16330..16762
                     /note="PGK pA"
     primer          14817..14840
                     /note="LRPCR RAF5"
     misc_feature    12703..12756
                     /note="T2A"
     misc_feature    12753..12753
                     /note="A to G to kill RsRII site"
     misc_feature    12757..13782
                     /note="Cre"
     misc_feature    11668..11707
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    11668..11683
                     /note="FCHK QC prefix region"
     misc_feature    11684..11701
                     /note="FCHK QC critical region"
     misc_feature    11702..11707
                     /note="FCHK QC suffix region"
     misc_feature    16859..16879
                     /note="B2 Gateway"
     misc_feature    10666..16909
                     /note="Synthetic Cassette"
     misc_feature    16910..20931
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaatagtg aaaccaccaa actagactac tccaagggaa taaaaaggtt
     4321 attttggaac aaaactgtct cagagagtag agggcaatga cttggcagga aagcaagcag
     4381 attttatatg acagggtagg agtcttgaat gcagagatca gcatgcctgt ctcctgggat
     4441 taaaggtgtg taccaccatg cctggatcta aatttagctg ggcaggatct tgcccgaagg
     4501 tcccacttcc ttaatctgtt atctcctaga acacaggatt ttgctccatt tcacttcctg
     4561 gtaccccttt aatactcaaa ccacatatta tatatttttt ttcctttcta agcttgctat
     4621 gtttgttcta acttcttttc ataagactta accagagaac aaagtctctg ctgggctttt
     4681 ttgaaacttc ctttgtcaaa gcaattaatc tgaatagcct caccttagcc tcaggcagac
     4741 ttttcagaca agggcaaaag tagccacatt cttcgacaaa ataccacaaa aatagtcttt
     4801 atgccacatt ctgagattct tctcctttga aatctcttgg gccaggtcaa caccgttcaa
     4861 attactccca gtaacagtct tccatattcc tactaggatg acccattaag ccccatttaa
     4921 agcattccac tgtgttccaa atccaaagtc cccaaatcta cattctttca attaaaagca
     4981 tggtcaggcc tattgcagca ataccccact cccagtacca acttctgtct tagttagagt
     5041 tttactgctg taaacagaca ccatgactaa ggcaagtctt attaaaaaaa aaaaaccaac
     5101 atttaaatgg gacgggctta caggttcaga ggttcagtcc attatcatca aggtgggagc
     5161 atggcagtat ccaggcaggc atggcgcagg cagagctgag agttctacgt cttcatccaa
     5221 aggctatagt gcaagactgg cttacaggta gctagggtga gagtattaag cccacaccca
     5281 catgacacac ctactccaac caggtcacac ctattccaat aaggccacac ctccaaatga
     5341 tgtcactccc tggcccaaga atatacaaac tatcacaagt ctctagcaga cataggttgt
     5401 ttgagctaat tgggaacaag gtatactttg cttgcattag gctcagagaa tggcaatagt
     5461 ggctttccct gacaaatagg gacccaaatc acaaggtgtc tgaggcctgg ctgggtttag
     5521 gtttcagttt acctggtaag agttctgttc acctggcttg ggcctgcggc catcatctta
     5581 ggacatcatc agtagcattg ccttttgtgg gccctgcttt gtgacataac agataagttc
     5641 tcaacaaatg gtggagtgga tagatggact ggaaggatga tggaatactg aaatggaggc
     5701 ttgaatgaat tttgaaagtt ctggagccat agatcctttt atgtttggga tctgtcttct
     5761 gatatcttct tcaatttctt tagtgacttg aagtttttat catccaagta tttcgcttgc
     5821 ttgattagca ttaacacaag ataccttata ttatttgagg cttttgtgaa gggtgtagtt
     5881 tcccttacct gtttctctgt ctgtttgcca ttggtacata ggagagctac tgacttaatt
     5941 ttgtatccag ctactttgct tgaaactgtt tatcagatat tggagttccc agtggaattt
     6001 ttaggatcac ttatatatac tgttatatca tctgcaaata aagaaacttt gacttcttct
     6061 gttctaattt gtattccctt gatctctgtc agttgcctta ttgctctagc taagacttca
     6121 agtactatat tgaataggta tggagagagc agacaacctt gttttgtttc gaattttagt
     6181 ggaaatgctt tgaatttatc tacatttaat ttgatgttgg ctactggctt gctataaact
     6241 cactttactt atgtttaggt acgtttcttg tagctctagt ctctccagga cttttatcag
     6301 gaaggacttt ggattttgtc aaagggcttt ctgcatctaa agagatgatc atgtggtttt
     6361 ttttaaattt gagtttattt atatggtggg atatatctat atctatctat ctatctatct
     6421 atctatctat ctatctatct atctatctaa tctctatcta tctatctatc tatctatcta
     6481 tctatctatc tatctatgat atattgatat atgtatgttg aaccatccct gtatctctgg
     6541 gcttaagcct actttatcat ggtggatgat atcttttgat gtgtcttgga ttcagtttgt
     6601 aagtatttta ttgaattttt tacatgaata ttcataaggg aaactgacct gtaattatct
     6661 ttctttgttg agtctttcta tggtttgagt atcagggtat ttgtggcctc acaaatatga
     6721 attggacaat ggtccttctg cttctatttt tttttttgga ataatttaag gagtattagc
     6781 attaactctt ctttgaaaat ctggtaaaat tctgagccat gactggccat gatcattttt
     6841 ttggttggaa gattttttaa tccctgagac tgcttctatt tcactaggtt tgtttaattt
     6901 tcttatcttg atttaacttt gttaaaggta atgtataaaa aaatatccat ttcttttaga
     6961 ttttccaatt tggtggagtg taggtcttta aagtattttc ttgtgatttg tctccttttt
     7021 gtttctgatt ttgttcattt gggcattcac ccttagcttt ttagttaatt tggataaaaa
     7081 atatgtcaaa tttctcaaag taccaactct ttgtttcatt gatttgttta ttttcctttc
     7141 tttctatttc attgatttca gccctgagtt tctttcttgc cttctactcc tcttgggtgt
     7201 gattactttt ttttttcttt tctaaagctt tcaggtgttc tgttaagtta ctagaatgag
     7261 attgctccac attttttttc atttttctta tagacattta ctgctataaa cttgactctt
     7321 tgcactgctt taactgtgtt ccagaagttt gggtacgctg tgtactcatt ttcactcaat
     7381 tctagaaagg ttttaattta tttcttaatt ctctcttggt ctattttcca tctagtagag
     7441 agttgttcag tttccatgaa tttgtcactt tttgttgttg atatctatct ttaatccatg
     7501 gtggtaagag aggatgcacg gagttatttc aatttcttta atatcttttg tatcttttat
     7561 tgctttgtgt ctgaaagtcc catgaggtgc agagaagaag gtgcattctt tttctgtcta
     7621 gttgaaatgt tctgtagaca tgtattggct tcatttagtt tttaatgtct cttaactcca
     7681 gtattttttt gttgttaagt ttttgtctgg atgacctgtc cattgtgaga gtttggtatg
     7741 gaagcctccc actattggtg tgtgaaggtt aaactgtagt agtgttgctt ttacaaatgt
     7801 gggtgtactt gtgtttgtac aagataacaa aatggctaca ccagcttgct tcttaggtct
     7861 atttccttgg aatatatttt cccatgcttt tacactgagg tgatgtctat ccctgatgtt
     7921 gaggtgtgtt tcttagctgc agcaggatgg atcctgtttt gcctccattc tgttattctg
     7981 tgtcttttat tgggggaatt gagaccactg ataatgagag atatcaatga atagcacttg
     8041 ttgatgccta ttattttgtt gttgttgata ctgtgttttt tccttctttt gactttgcat
     8101 gtctgggatt acttattccc tatattttct tggatgtagt tatcctcttt aggttgatgt
     8161 tttctttcta gcatcttctg aaaggttgga gtcttagaca gatactgatt aactttggat
     8221 tttctcgtga aatatcttat tttctccagg tattgtaatt gaaagtgttt atgagtataa
     8281 tagtcagggt ctcttggagt cttgcagcac actgtccaga tacttctggc ttttagagtc
     8341 tccactgagt agtcagtata agtctaatat gtctgctttt ataaattacg tattctttag
     8401 ctcctttgaa gcttctaaca ttgctttaac aattttaaca atttttgttt gttctgaaca
     8461 tttagtgttt tgattattat gtgccaagga agtctttctt ttgtggtcga gtctatttgg
     8521 agttctgtga gcttctaata ctttgctagg catctccttc tttagattat ggaaactttc
     8581 ctctattatt tcattgaaaa tatattctat gcctttgacc tatgtttctt ctcctacctc
     8641 tatttctatt attcatagat atgctctttt cgaagtattc caggtttctt gaatgttttg
     8701 tgtcaggagt tttttagttt taatactttc tttgaatgat gtatccactt aatctattgt
     8761 gtctttgatt gcgatcctct cttccatctc ttatattctg tttgctgaag cttgccactt
     8821 tgcttcctgt tcgtgttcct aaatttttca ttttctgatt tcctacagat tggtttgatt
     8881 ctttttccac tttcaggtct tgaagggttt gattcatttc tttccactgt ttgttggtgt
     8941 ttacatagac ttcttcaaga gacatattca ttttgtcttt actctgtcac attcataaat
     9001 gctgttttga tctttttctt gtgcctcaga taggttgcaa tattcagtat tcaatattct
     9061 gggctctagt ggagtcatgt tgtcttggct attattggtt gtggttttac agtggcatct
     9121 aggcatctga gatttggaag actgtagctc ttggtgctgt tatctggtct tgtctttgtt
     9181 gggtgggtgt cttgttcctc ggtttttttt tttcacctct ttattttagg agggtgtggt
     9241 atctgtgtgt tgcctgggtg gtgtggtcac tggagatttc aagtaaaatg gaatcttttt
     9301 tatgggtatt gggagctgac acttatacct ttggagaggg tttggactgg caagactgag
     9361 tggggtccat agactgagga aagcaaggtg ttccacaagg atttacttag tcccctggaa
     9421 atgggtacgg actgggggaa ggccacagca gaaggtctgc tacagagctg gggatgagac
     9481 tgggaggttg gacttggagg aggaatggag agagaataaa gatctatagt cagtctactt
     9541 gcttccctgg tcagagtggc cttgggttct caggaagtgc ctgctggagt tggagactgg
     9601 gacaaagcaa tgggttgtgg taaggaagtc tggatgggaa tatctgtgcg atccatgggt
     9661 gatagggggg aaagtgttct gctgcagatc tggagatgaa cctgggggat ttgaactgta
     9721 ggagcagaag gagacttaaa ggtcttcagt cagctagctg cttccctggc aggaatattt
     9781 acttcttagc tcaagggcct ctgatgatta acactctgac atattcccac cttctcatat
     9841 agtgactgac acatgattct caacaagtat taggtcttaa acttcttcta attcacatta
     9901 gtggctatgg ctccaaagag atttccctag ctttcctcca ttctctattc tatactccaa
     9961 agagtggctg agtccaaacc taccctgtcc ctttctttac tatagtaaca agatattata
    10021 cagttaatga ttttttgaga caggggcctc aagcttacta tgttgacaaa gatgaccttg
    10081 aacttgtgat acttgagata gtgcctatat ctcctgaagg ctagtattat aggcaaacac
    10141 tctatcaact aagcagcaca ccgccaccac cccagtccat gtttatctgt ttttatgctc
    10201 aggtgcacac ttgtgtatta aatatgttag attataatta tataataacg atttcagtga
    10261 ctaatgtgct aattgaatac aactgaacaa ataggaaaca ggcccataga gggatactta
    10321 ggactgattt atttcaaatt ctgtgagaga gagacagaga gagagagaga gagagagaga
    10381 gacagagaga cagagagaca gagagacaga gagacagaga gacagagaga cagagacaga
    10441 gacagagaca cagacacaca gagagacaca gagagacaga gaggggggaa aggagagagc
    10501 ctgagaggct atgaatgaga tagtatcagg atcatcttag tgtccttcgt cttcttgtat
    10561 aacagcagaa atttgggatt ataattggta agttctgttc caccctgtcc tgccttaccc
    10621 catttcatag gctctaactt cttcctcttc taccagctca agttaaaggc gcataacgat
    10681 accacgatat caacaagttt gtacaaaaaa gcaggctggc gccggaaccg aagttcctat
    10741 tccgaagttc ctattctcta gaaagtatag gaacttcgaa ccctttccca caccaccctc
    10801 cacacttgcc ccaaacactg ccaactatgt aggaggaagg ggttgggact aacagaagaa
    10861 cccgttgtgg ggaagctgtt gggagggtca ctttatgttc ttgcccaagg tcagttgggt
    10921 ggcctgcttc tgatgaggtg gtcccaaggt ctggggtaga aggtgagagg gacaggccac
    10981 caaggtcagc cccccccccc tatcccatag gagccaggtc cctctcctgg acaggaagac
    11041 tgaaggggag atgccagaga ctcagtgaag cctggggtac cctattggag tccttcaagg
    11101 aaacaaactt ggcctcacca ggcctcagcc ttggctcctc ctgggaactc tactgccctt
    11161 gggatcccct tgtagttgtg ggttacatag gaagggggac gggattcccc ttgactggct
    11221 agcctactct tttcttcagt cttctccatc tcctctcacc tgtctctcga ccctttccct
    11281 aggatagact tggaaaaaga taaggggaga aaacaaatgc aaacgaggcc agaaagattt
    11341 tggctgggca ttccttccgc tagcttttat tgggatcccc tagtttgtga taggcctttt
    11401 agctacatct gccaatccat ctcattttca cacacacaca caccactttc cttctggtca
    11461 gtgggcacat gtccagcctc aagtttatat caccaccccc aatgcccaac acttgtatgg
    11521 ccttgggcgg gtcatccccc cccccacccc cagtatctgc aacctcaagc tagcttgggt
    11581 gcgttggttg tggataagta gctagactcc agcaaccagt aacctctgcc ctttctcctc
    11641 catgacaacc aggctcccag gtcccgaaaa ccaaagaaga agaacgcaga tcgcagatcg
    11701 cagatctcta gagtcaccga gttgctttac cggatgaaga gggccgaaac atactgtcca
    11761 aggcccttgc tggcaatcca cccaactgaa gccagacaca aacagaaaat tgtggcaccg
    11821 gtgaaacaga ctttgaattt tgaccttctc aagttggcgg gagacgtcga gtccaacccc
    11881 ggaccggtgc atgtggaccc caagaaaaag cggaaggtgg accctaaaaa gaaacgcaaa
    11941 gtggatccta agaaaaaaag aaaggtgcac gtgtccatgg tgtccaaggg cgaggaactg
    12001 ttcaccggcg tggtgcccat cctggtggaa ctggatggcg acgtgaacgg ccacaagttc
    12061 agcgtgtccg gcgagggcga aggcgacgcc acatacggaa agctgaccct gaagttcatc
    12121 tgcaccaccg gcaagctgcc cgtgccttgg cctaccctcg tgaccacact gacctacggc
    12181 gtgcagtgct tcagcagata ccccgaccat atgaagcagc acgacttctt caagagcgcc
    12241 atgcccgagg gctacgtgca ggaaagaacc atcttcttta aggacgacgg caactacaag
    12301 accagggccg aagtgaagtt cgagggcgac accctcgtga acagaatcga gctgaagggc
    12361 atcgacttca aagaggacgg caacatcctg ggccacaagc tggagtacaa ctacaacagc
    12421 cacaacgtgt acatcatggc cgacaagcag aaaaacggca tcaaagtgaa cttcaagatc
    12481 cggcacaaca tcgaggacgg ctccgtgcag ctggccgacc actaccagca gaacacccct
    12541 atcggcgacg gccctgtgct gctgcctgac aaccactacc tgagcaccca gagcgccctg
    12601 agcaaggacc ccaacgagaa gagggaccac atggtgctgc tggaattcgt gaccgccgct
    12661 ggcatcaccc tgggcatgga cgagctgtac aagcctgcag gcgagggcag aggaagtctt
    12721 ctaacatgcg gtgacgtgga ggagaatccc gggccgtcca atttactgac cgtacaccaa
    12781 aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac
    12841 atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct gtccgtttgc
    12901 cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc agaacctgaa
    12961 gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa aactatccag
    13021 caacatttgg gccagctaaa catgcttcat cgtcggtctg ggctgccacg accaagtgac
    13081 agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa
    13141 cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg ttcactcatg
    13201 gaaaatagcg accgctgcca ggatataagg aatctggcat ttctggggat tgcttataac
    13261 accctgttac gtatagccga aattgccagg atcagggtta aagatatctc acgtactgac
    13321 ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac cgcaggtgta
    13381 gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc cgtctctggt
    13441 gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg
    13501 ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga agcaactcat
    13561 cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg gtctggacac
    13621 agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat accggagatc
    13681 atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg taacctggat
    13741 agtgaaacag gggcaatggt gcgcctgctg gaagatggcg atctcgagcc atctgctgga
    13801 gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc taagaagaac
    13861 agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga tgctgagccc
    13921 cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc gatgatgggc
    13981 ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc gaagagggtg
    14041 ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg tgcctggcta
    14101 gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa gctactgttt
    14161 gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat ggtggagatc
    14221 ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca gggagaggag
    14281 tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt tctgtccagc
    14341 accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa gatcacagac
    14401 actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca ccagcggctg
    14461 gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg catggagcat
    14521 ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct ggaggcggcg
    14581 gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga ggagacggac
    14641 caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa gtattacatc
    14701 acgggggagg cagagggttt ccctgccaca gcttgatgaa gatctgagct cttaattaat
    14761 gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca
    14821 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
    14881 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    14941 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    15001 ccgtttaaac taactttaaa taattggcat tatttaaagt tacatatggc ggccgcccta
    15061 aggctcgacc tcgaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
    15121 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
    15181 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
    15241 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
    15301 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
    15361 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
    15421 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
    15481 cggggcgggc gcccgaaggt cttccggagg cccggcattc tgcacgcttc aaaagcgcac
    15541 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca tccatctaga
    15601 tctcgagcag ctgaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
    15661 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
    15721 gcgccacacc gtcgatccag atcgccacat cgagcgggtc accgagctgc aagaactctt
    15781 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
    15841 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
    15901 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
    15961 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
    16021 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
    16081 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
    16141 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
    16201 gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
    16261 cgcacgaccc catgcatcga tgatatcaga tccccgggat gcagaaattg atgatctatt
    16321 aaacaataaa gatgtccact aaaatggaag tttttcctgt catactttgt taagaagggt
    16381 gagaacagag tacctacatt ttgaatggaa ggattggagc tacgggggtg ggggtggggt
    16441 gggattagat aaatgcntgc tctttactga aggctcttta ctattgcttt atgataatgt
    16501 ttcatagttg gatatcataa tttaaacaag caaaaccaaa ttaagggcca gctcattcct
    16561 cccactcatg atctatagat ctatagatct ctcgtgggat cattgttttt ctcttgattc
    16621 ccactttgtg gttctaagta ctgtggtttc caaatgtgtc agtttcatag cctgaagaac
    16681 gagatcagca gcctctgttc cacatacact tcattctcag tattgttttg ccaagttcta
    16741 attccatcag aagctggtcg agtaacttta aataattggc attatttaaa gttaggcgcg
    16801 ccataacttc gtatagcata cattatacga agttatgtcg agatatttaa atattagacc
    16861 cagctttctt gtacaaagtg gttgatatct ctatagtcgc agtaggcggg gtttctttcg
    16921 aaggaagaaa ggtgtgaggt tcctactcca ccttctgaca gagagcgttg tgtcgagtct
    16981 caaaggtgtc actgacattc gagtgacttg gcttcttctg tctgactcca cagccaccta
    17041 ccagcagcca gctcacttta tcttttccaa gagttgagtc ctgaataatt ataggacagt
    17101 ctgagatatc agaaccgcta ggaaagtaga aaaacggtag agtagttcag gcaagaaagt
    17161 aaggaaagtg gatgagtgag agagagcaag agtctcatcc acaggtagag actgtgccag
    17221 tatgatgctc cttggtaaag gtaggaaaga aactctaggc ttcatgattc agacttctct
    17281 gtgttgccta gaagcatctg ttcacatagc gtcactcccc tttaattcag acctacacgg
    17341 gtaccattga agtcctagat tttagagtca gtcacatctg ggaccactcc tgatatagtc
    17401 actgtattag ttatttttat tgtcccacac tatagtgata agaatgtgct tttgttttaa
    17461 tcccaggtgt ggggacgtgg ggctcctttg gactgttcac agcagctgac tatgatttgc
    17521 ctcatgccct agcagaggtg tggttttgcc acttacagat agtttctgca actgtgtaat
    17581 gtttagaatt ctaggtaatt ttttagaagg actttcatat agatgctagg tcccagggag
    17641 tcaaggtttg tggttggtgg tagtttagaa aagttgtttt tggtttgtta gtagttgtgc
    17701 tcaaagaaga aacaaaaggg aaaaaaaatt agattgttag attcagggat ctctctctct
    17761 ctctctctct ctctctctct ctctctctct ctctctctct ctctctctct ctctctctct
    17821 ctctctctct ctctctcccc tatcattttt ctctcttcta tctagtgtta gggggtaaaa
    17881 ttggcaggag gtagataagg cggtgaaaag aagaacccac aaagtagcta agaccagcta
    17941 caccatacca aaacagcata agataggaca tgtaagaata cagtccatca cgacaggcaa
    18001 ggcgtggcaa tggaagtgtc agatggcttt tcacaccatg tccacagtca ggaagcagag
    18061 aaatgaatgt tggtgttcaa aggcttcctt cttttcacct tcattaagtt ggaggcctca
    18121 gcttatggaa tggcattgcc cacattgagt agagaccttc cctactcatt taaatagctc
    18181 tggaaacatc cttaacagat gctcacagat gtgtctctta ggtgattcca tagcccacca
    18241 aactgacaac cgagtttaat tataacagcc tgagtttaac tgtcatatct atggctaact
    18301 aagatgttta atttctccaa gctatgcttt cttaaatgta aggcaaaata attgttctta
    18361 caatgctata aggaataaat gaatgatgca cctaatccat ataacacaga tcctggcaaa
    18421 ttaatcacaa atggtaaata gcaatggtaa ggtaatagca tctaagttag ttttggtttc
    18481 tctaactctg gaaacaactg gacttggggg accatttaat gaaattgtct acaacttcca
    18541 gaactctagc taccccatgg tccatattta aaagtgctat ccttttgccc caatgtacac
    18601 agttttccag aatagttcaa acctacagta gcaatttttt tctgtgacca tgttatggtc
    18661 agataactct cctggcaggc ataaagatat tcttaagagg caaatatact ctccatttgg
    18721 ggatagataa tttcaatatt agctgttgaa agggaaacac gttggctcat ctaatcccag
    18781 cacttgggag actaaggcag gaggataaaa tactttggag ccagtatagt cttcctagtg
    18841 agaccctgtc tcaaagttaa caactaaaat gggagtaact tccactaatc cttccaaaag
    18901 taactaaagt caagggctta tggagaaatt attcttatgc ccaattacct gacactcact
    18961 gtcttctcac cgtggctcct atttatatta tatttgtgat atttgtttca gatgcaagaa
    19021 tgtttcacct caagactgat gtctcaagta gctatgcaga agaggaggca gaaaggttgt
    19081 aagaggcagt agggatggga gacaccaagg aagcaatgcc ttccagacat aacaggattg
    19141 atgcacatat gaactcatag agaccatgtg tcttagtttg ggttttactg ctgtgaagag
    19201 acactatgac caaggcaact cttataaagg acaatattta actaaggctg gcttacaggt
    19261 tcagaggttc aatccattta catcaaggaa gtagcatggc agcatccaag caggcatggt
    19321 acaggaagag ctaagagttc tacatcttgt tctgaaggca aacaggagca gactggctaa
    19381 ctgacagcta ggagtagggt cttaaagctc acacccacag tgacacacat actccaacaa
    19441 ggtcacatct cctaatagtg ccattcctta ggccaagcat atacaaacca ccacaccatg
    19501 acagcatgtg cagggccagg acaggtctaa gccagatggg atcccagcac tgataggggg
    19561 aagtggacat gagcctccat ccctaaccca aaagctattt ccaagtgtca atctcttttt
    19621 tttaaagaat ttatttattt gtttaatgta tatgagtaca ccattgccct cttcagacac
    19681 accagaagag ggcatcagac cccattgcag atggttgtga gccaccatgt ggtttctggg
    19741 aattgaactc aagacctcca gaagagcatc agtgctctta accactgagc ctaagtgcaa
    19801 tggaaaattt agttttgtcc agtgaagcct tactgggtat acaaaccata ctgaaggaca
    19861 ggcccatgcc tagcagtagg tggccaacac aaaatgaact cagtggtatt ctggaaagtt
    19921 ttgttgtttt gtgtgtgtgt gtgtttgtgt gtgtgtgtgt gtgtgtgtct cataatgatt
    19981 tgtttggaca tttataaaaa aaactttgtc tggtcttttg ttaatatatt atggcttctg
    20041 attttatgta tctgtatgtg tatgtgtttc tcattctttt tctttgtctt gtcatttgtt
    20101 tgttttattc tggtttgttt gtttgcctgt tttctaaaga aatagagaat gaagatgcag
    20161 ggttggatgg gtggggagat ggggaagatc tgggagaaga taaaagaggg aacatcatga
    20221 tcagaatata ttatatgaaa caccaatgac aacaaaaaca ccgaggtgta aaaactaaga
    20281 gaagggcaat gtaacactca agatactgta accttcattt tggatatcag accattaaaa
    20341 caatgggcac tgcaatggtt tgaatagaga taccccccct cagtcccctg cccaggcaca
    20401 tgtatttgaa tgcttggtta gggagtgttg cttctgtaca gggattagga agaacagcct
    20461 tgttagagga agtgtgtcac tgggggtggg ggtgggggtg gggagctttt gtagtttcaa
    20521 atgctcaagc catgtcaagt ctctctctct tcttgttgcc tggcagtcca gatatacagt
    20581 ccttctgcaa caccatttct gcctgcatgc tgacatgctt cctgctatgc ggataatggg
    20641 ctaaacctca gaaactataa gccagcccca agttaatgct tttctttata agaatttctg
    20701 tgaacatgat gtctcttcac agcaatagaa cactaagaca gaccccaaga aattggaatt
    20761 tctccaggaa tctgatattg gtagagtaaa aggctagaga aaggacttgt tgccagggaa
    20821 gagatttcac atttatcaca acttgccaga acaccttgcg ggagagatta aaaccatgtg
    20881 ggaccatgct ttgattaaga ctgcttactt atgtgtacct cttgctttct a
//