LOCUS       194916#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        22734 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 194916
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000102832
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7040..7054
                     /db_xref="ENSEMBL:ENSMUSE00001027480"
                     /rank="4"
                     /note="ENSMUSE00001027480"
     exon            5373..5390
                     /db_xref="ENSEMBL:ENSMUSE00001022985"
                     /rank="3"
                     /note="ENSMUSE00001022985"
     misc_feature    4275..9768
                     /note="5 arm"
     misc_feature    9796..9820
                     /note="B1 Gateway"
     misc_feature    9833..9880
                     /note="Frt"
     misc_feature    9881..10380
                     /note="mouse En2 intron"
     primer          complement(9949..9970)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9845..9868)
                     /note="FRT3"
     primer          14046..14064
                     /note="seq primer NF"
     misc_feature    14106..14113
                     /note="RE sites"
     misc_feature    15906..15939
                     /note="loxP"
     misc_feature    complement(14114..14145)
                     /note="Rox"
     misc_feature    complement(15866..15897)
                     /note="Rox"
     misc_feature    14146..14167
                     /note="polylinker"
     CDS             14726..15322
                     /note="puromycin N-acetyl transferase"
     misc_feature    14841..14846
                     /note="Mutated RsrII site"
     intron          10381..10755
                     /note="mouse En2 intron"
     misc_feature    10753..10755
                     /note="Splice Acceptor"
     exon            10756..10809
                     /note="mouse En2 exon"
     primer          10656..10677
                     /note="seq primer FCHK2"
     misc_feature    10810..10932
                     /note="1Dleader"
     misc_feature    10933..10984
                     /note="F2A"
     misc_feature    11080..11796
                     /note="EGFP"
     misc_feature    10990..11079
                     /note="3X NLS"
     misc_feature    13862..14100
                     /note="SV40 polyadenylation site"
     misc_feature    12895..13839
                     /note="ERT2"
     promoter        14178..14689
                     /note="PGK promoter"
     misc_feature    15433..15865
                     /note="PGK pA"
     primer          13920..13943
                     /note="LRPCR RAF5"
     misc_feature    11806..11859
                     /note="T2A"
     misc_feature    11856..11856
                     /note="A to G to kill RsRII site"
     misc_feature    11860..12885
                     /note="Cre"
     misc_feature    10771..10810
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    10771..10786
                     /note="FCHK QC prefix region"
     misc_feature    10787..10804
                     /note="FCHK QC critical region"
     misc_feature    10805..10810
                     /note="FCHK QC suffix region"
     misc_feature    15962..15982
                     /note="B2 Gateway"
     misc_feature    9769..16012
                     /note="Synthetic Cassette"
     exon            18254..18300
                     /db_xref="ENSEMBL:ENSMUSE00000216409"
                     /type="targeted"
                     /rank="7"
                     /note="target exon 1 ENSMUSE00000216409"
     exon            17589..17756
                     /db_xref="ENSEMBL:ENSMUSE00000216408"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 2 ENSMUSE00000216408"
     exon            16382..16597
                     /db_xref="ENSEMBL:ENSMUSE00000216411"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 3 ENSMUSE00000216411"
     misc_feature    16013..18482
                     /note="Critical Region"
     misc_feature    18506..18539
                     /note="Downstream LoxP"
     misc_feature    18483..18562
                     /note="synthetic loxP region"
     exon            19143..19987
                     /db_xref="ENSEMBL:ENSMUSE00000662742"
                     /rank="8"
                     /note="ENSMUSE00000662742"
     misc_feature    18563..22734
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaagcaac tccttcaaca tcctgtattt ctaacaagct ttgggtttat
     4321 ttatatggga ctggtccaag gaacacactt tgagtctcac ggggcagact atgactcctg
     4381 caagaagcag tcattaattg tactacccct taaattaaat aaataaataa ataaacaaaa
     4441 aaacaaacaa ataatgaaag catcacttaa aagagaaaag aggagagtct atggtatagt
     4501 tgagtggctg aagtgcctcc catcaaggtt gacaacaacc tgcattccct ccccaggccc
     4561 tacacgtgaa aggagagaag ccgttcctac aggttgccct ctgacctccc aatcccgaag
     4621 cacacatgca cacacaggca cacactaaat aaatgtaaac aaacataata aagggaaaga
     4681 taaagacgga ggagggaggt aaggagaatg ttgaagactc aagcactgta gatatgtgct
     4741 ttgtctgctc tggggatgca gaacgtctca ccctgcactg agactgttca taagcaaaac
     4801 gctcgctcgg gcggtgcagg ggaactggcc actgtgacag acccaagtca gtgttgatga
     4861 ggggacccag gatagtccga tgcacaggct ctagttgcta gtcgtcaggc tttcaaatat
     4921 ccccacctct gtctcaagca gaaaacaagt ggaaattttg agctcagcaa agacagaatg
     4981 agaaagaatc ctaacacctg cacccaccaa gggcttgttt tccgtctgaa gtcagagagc
     5041 agttttcaaa atggaggggc agctgcaaag ctccgtggct ttttcttgag atgacccccg
     5101 actttcttga cttctatttg ttaaacactg tgcattcaca tcgaatgcta gaagtttcct
     5161 cgtcccgctt cctcctgaat tgcctgggat cctctgcttg atgccctgta ggaaacgtcc
     5221 tttcctgtgg tatagaaatg actgttatgt gaccgtgggt gagaaggact tgagatacca
     5281 gttctgatca gacaggagca cagttctttt tgtttggaat ttcaggcatt cacagcacat
     5341 actaactcat gtctcttctg tttgttttcc agttggcact tgccaggacg gtaagatatg
     5401 atttctttct ttgctatgaa agttatgcca ggactggact tagccgtcat gctaaataac
     5461 agtgatctgg tctataactg atggacagca gtgatcccat gcaattacag tagagctgga
     5521 aagctcccat cacctggtat ttcactgtat ctgctttgtg tgcccataca cagacacttc
     5581 ccatttgtgt tgtgagcgtc tgagcatcta attacatgct gcagagcttc atgggctgga
     5641 cataaataac actttacact gtatagtcta ggtgaacagt ggatacaaca gtggacactc
     5701 tgtagtctag gtgaatagtg ggcacaacac gctacaccat atggttgagt gaacagtggc
     5761 ctctgccatg taggtttgtg tacatgtgtg ccgtgatgtt tgttcaatga ccaaatcact
     5821 taagaacgcc tttctcagaa ctgaccgtat tggagaacag tctggtcaag gcttccctta
     5881 gacattccac ggacagcagt gaaagatttt tttcctcctt tccattatag atgtacccca
     5941 ggttagagaa ccaagagaac cagaaaataa tgtgtctgaa caatgtaaag ttgtttaaaa
     6001 gtttaaagtg gagctgggaa ggtgactcag ttggtgaaat gtttgcctgg caagaatcag
     6061 gcacaatcca agcactggga aggcagagac aggaggatcc ctggggcttc caagcagcca
     6121 gtatagccta gtcttcaagg tccaggttca gtgagacacc ttgtctcaaa agcagtaaga
     6181 tggagagcta ttgcagaaga cacctaatgt ctacctctac acgcgcacac acactcacac
     6241 acactctctc tctatctacc tctctctctc tctctctcac acacacacac acacacacac
     6301 atacacatgc acacgcacac gcacacgcat gcatgcactc acaggaatgc taacatacac
     6361 atataaacaa aaagttaaaa gcatcagaca cggtagttca tgcctttaat cccagtactt
     6421 aggaggcaga ggcaggcaga tctctgtgag ttcaaggcca gtctggtcta caaagtgagt
     6481 ccaggacagc cacagaaaaa tccagtctca aaaaaaaaaa aggtagtatc agtggttttc
     6541 attcttctgc ttttcctgtt tgatatgagg ttggtagagg atgtgagatt ggccagaatg
     6601 cgtgcatagg gtgaccctgg ttaagctttg ggatggcatc tgggtgcaca ctcataatca
     6661 cgtgctcgcg tggtctgaat gtgggtgtct acctgcacac tagcccaaac tttactttct
     6721 caggtaggga aatagagtca aggccatccc aaacctggaa gcctccctgt ccagtcatca
     6781 cctctgaaca gagaagcaac attgtgttta aaagcagcaa cttgtgacag gctccttccc
     6841 cttcactgta tacctggtgc atagccaaca gatatagaac gaagatgtaa ggctccgaag
     6901 tttgtcatat gcagtcaaag gaacttcaag atccattgtg ctcttaagat tgaggggagg
     6961 gtcctaactc atagaagtag ggtggatctt agtcactgat aagaatatta ctaacggctt
     7021 cgtactgctt ccctttcaga tgccgagaac attggtaaga agatttgctc tattcggccc
     7081 ctctcggatg tagaataaac taatgagagg gaccaccaca aagggaacca gagctaagag
     7141 agggagacgg ggatcgtcgg tctgagatgt ttctggttgt gttgctagcg tccatgtctg
     7201 cttgtgtgct tccctgagca cactgttcgg gaaaggcaca tagacttgtt tttgtgtgaa
     7261 ccgtaagttc cttgagagcc aagacagcat ttcatttgcc tgaccttgga tgctctatga
     7321 agtatctgag tgagtaaatg gacagaatat taacattttg ctccttcttt gtcctcaaga
     7381 agccaactgt cttgtaaggg acaccggtat ttgatgctgc agtgtactgc acacaatgag
     7441 taggtgtgga aggaaaaaat gaaatataaa aagaatggca aactttgcaa agcgtaactg
     7501 aggcaggcag caaggggcag gtggctcttg aactgggttt taaaggtttc atttgtactt
     7561 tagggcagaa ggatgcttga ggcagaggag ataaatcctc ccatgaggct cgactgccaa
     7621 gttggaatcc tgactcagtt tcctatttgt tgagtgataa tagatagctt acttatatct
     7681 gggctattcg atcattgtgt ggaaatgcaa acaccctaca gccttgttgt gaaggcttaa
     7741 tgattcatcc aagactcaca cggtaccata gaagtcagta gatggcattt ggcagttcta
     7801 tagaagtttg ctgattatag ggaataaaca tgggggtggg ggtatgtgga gagatggctc
     7861 agcagttaag accccttgct actttttcag agaaccaaag ttcagttctc agtacccatt
     7921 tccatcctcc tctaggagca cccacacatc tctgtgcata caaacaaaca aacaaacata
     7981 cacataacat ttcttttaaa tctctttttt taaaagggat taaatgggtg tagggctaca
     8041 tgaactttaa tctcagcact ctggaggcag aggcagctag aactctgagc cggaggccag
     8101 cctagtctac atagcaagtt ccaggtcaac caaagctaca tagtgaagcc tctctctcag
     8161 ggaaagaggg ggtgtgaaga gatgtatacc ttagaagctg agcacagtca gaccttgtta
     8221 gtatttgaat agaagaatga gtgttaaaat tttgttgagc atatatgttc taactattgc
     8281 cctaaataag ggaggagagg cttagaaatg acagtccaat caatgagggg aaaagagttt
     8341 ataagaagct caatttctta accattgtga aatgccatgg atgtttgtaa agggggatgt
     8401 ggacaaactt cacgaacaca tctgtttcta tgcatgggta tcctgttttt ttatgagcat
     8461 gtgtaaacac tctggcattg aacttggacc aatagggccg taagctcttt tgtttttgtt
     8521 tttttttttt aacattcttt caaactagcc tcctaaacat aatatcactt taccttattt
     8581 atgatttttg attcacataa gctttctagg aaacatcgat ggtaaaaatt aagtcaaagt
     8641 aaccctctat tttcattgta cccagctccc agctggcggc tggtagagca gaatctgtgt
     8701 gtacaaagac gcagccgagt gactgctgaa tggctgagtg tgagcccagc actggtctcc
     8761 gtggtagcag gatctcaggg agaaagggag gcaagaaagc agcacttttc atgagcatct
     8821 gctacgtgaa tccatgttcg ccttcagagt cccttcgatt ctctcagatg cctctgaggc
     8881 agtcatttga cctccattcc cctgatgagg agacttgaag ctgggagcca tggtacatgc
     8941 ctgtaatccc agcacagccc tggttcctcc taccaaccaa agaagagaat ccattatact
     9001 cagagacaat gcaatgtcag gtcattcttt acagaaagag ccatcctctt aaactgtgca
     9061 cttaaaaagt gtcttgggtc attcagagtc aatgtagaca tatattggac atatatcgaa
     9121 taagacattt gatcttggtt acaagcagct tcaagcactt taaaggaaaa aaatcaaaag
     9181 ctggggatgt ggctcagaca gcagaacact tgcctggcct gcacaaagcc ctgggttcgg
     9241 tcctcagcac cacatgaact gagcatagtg atgtgcacct agaaccccag aactcaggag
     9301 ttagaggcag cggggtcaga agttgaagat tgctctcagc ttcatagcaa atttgagacc
     9361 agccttgact ccacgagacc ttgtctcaaa acaaaaagaa aaaaaaatta tgaaaaatct
     9421 atagacctga gagtcagtga ttcaaattca cagaagtcct tgtttctaag atcctgtgac
     9481 agctgtggaa gatggttctg gctgaagcag gggaaacgtc cttgagtttt ctgtctgatg
     9541 actctcgttt ccgtagttct ggcttatggg cacaagctcc tgtatgttga atttgaagta
     9601 tgtggattat acaaaagcat atctccggag gctgaatcca gacgtcacct ggggggctta
     9661 tggtttttga tgggttgtta tctattctgt ctggaagacg tttacttcac attgaagtgt
     9721 aaaccattgt tcttttctta ctgtttcttt ttcatttgca gatgatagaa ggcgcataac
     9781 gataccacga tatcaacaag tttgtacaaa aaagcaggct ggcgccggaa ccgaagttcc
     9841 tattccgaag ttcctattct ctagaaagta taggaacttc gaaccctttc ccacaccacc
     9901 ctccacactt gccccaaaca ctgccaacta tgtaggagga aggggttggg actaacagaa
     9961 gaacccgttg tggggaagct gttgggaggg tcactttatg ttcttgccca aggtcagttg
    10021 ggtggcctgc ttctgatgag gtggtcccaa ggtctggggt agaaggtgag agggacaggc
    10081 caccaaggtc agcccccccc ccctatccca taggagccag gtccctctcc tggacaggaa
    10141 gactgaaggg gagatgccag agactcagtg aagcctgggg taccctattg gagtccttca
    10201 aggaaacaaa cttggcctca ccaggcctca gccttggctc ctcctgggaa ctctactgcc
    10261 cttgggatcc ccttgtagtt gtgggttaca taggaagggg gacgggattc cccttgactg
    10321 gctagcctac tcttttcttc agtcttctcc atctcctctc acctgtctct cgaccctttc
    10381 cctaggatag acttggaaaa agataagggg agaaaacaaa tgcaaacgag gccagaaaga
    10441 ttttggctgg gcattccttc cgctagcttt tattgggatc ccctagtttg tgataggcct
    10501 tttagctaca tctgccaatc catctcattt tcacacacac acacaccact ttccttctgg
    10561 tcagtgggca catgtccagc ctcaagttta tatcaccacc cccaatgccc aacacttgta
    10621 tggccttggg cgggtcatcc ccccccccac ccccagtatc tgcaacctca agctagcttg
    10681 ggtgcgttgg ttgtggataa gtagctagac tccagcaacc agtaacctct gccctttctc
    10741 ctccatgaca accaggctcc caggtcccga aaaccaaaga agaagaacgc agatcgcaga
    10801 tcgcagatct ctagagtcac cgagttgctt taccggatga agagggccga aacatactgt
    10861 ccaaggccct tgctggcaat ccacccaact gaagccagac acaaacagaa aattgtggca
    10921 ccggtgaaac agactttgaa ttttgacctt ctcaagttgg cgggagacgt cgagtccaac
    10981 cccggaccgg tgcatgtgga ccccaagaaa aagcggaagg tggaccctaa aaagaaacgc
    11041 aaagtggatc ctaagaaaaa aagaaaggtg cacgtgtcca tggtgtccaa gggcgaggaa
    11101 ctgttcaccg gcgtggtgcc catcctggtg gaactggatg gcgacgtgaa cggccacaag
    11161 ttcagcgtgt ccggcgaggg cgaaggcgac gccacatacg gaaagctgac cctgaagttc
    11221 atctgcacca ccggcaagct gcccgtgcct tggcctaccc tcgtgaccac actgacctac
    11281 ggcgtgcagt gcttcagcag ataccccgac catatgaagc agcacgactt cttcaagagc
    11341 gccatgcccg agggctacgt gcaggaaaga accatcttct ttaaggacga cggcaactac
    11401 aagaccaggg ccgaagtgaa gttcgagggc gacaccctcg tgaacagaat cgagctgaag
    11461 ggcatcgact tcaaagagga cggcaacatc ctgggccaca agctggagta caactacaac
    11521 agccacaacg tgtacatcat ggccgacaag cagaaaaacg gcatcaaagt gaacttcaag
    11581 atccggcaca acatcgagga cggctccgtg cagctggccg accactacca gcagaacacc
    11641 cctatcggcg acggccctgt gctgctgcct gacaaccact acctgagcac ccagagcgcc
    11701 ctgagcaagg accccaacga gaagagggac cacatggtgc tgctggaatt cgtgaccgcc
    11761 gctggcatca ccctgggcat ggacgagctg tacaagcctg caggcgaggg cagaggaagt
    11821 cttctaacat gcggtgacgt ggaggagaat cccgggccgt ccaatttact gaccgtacac
    11881 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
    11941 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
    12001 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
    12061 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaactatc
    12121 cagcaacatt tgggccagct aaacatgctt catcgtcggt ctgggctgcc acgaccaagt
    12181 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
    12241 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
    12301 atggaaaata gcgaccgctg ccaggatata aggaatctgg catttctggg gattgcttat
    12361 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
    12421 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
    12481 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
    12541 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
    12601 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
    12661 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
    12721 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
    12781 atcatgcaag ctggtggctg gaccaatgta aatattgtca tgaactatat ccgtaacctg
    12841 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgatctcga gccatctgct
    12901 ggagacatga gagctgccaa cctttggcca agcccgctca tgatcaaacg ctctaagaag
    12961 aacagcctgg ccttgtccct gacggccgac cagatggtca gtgccttgtt ggatgctgag
    13021 ccccccatac tctattccga gtatgatcct accagaccct tcagtgaagc ttcgatgatg
    13081 ggcttactga ccaacctggc agacagggag ctggttcaca tgatcaactg ggcgaagagg
    13141 gtgccaggct ttgtggattt gaccctccat gatcaggtcc accttctaga atgtgcctgg
    13201 ctagagatcc tgatgattgg tctcgtctgg cgctccatgg agcacccagt gaagctactg
    13261 tttgctccta acttgctctt ggacaggaac cagggaaaat gtgtagaggg catggtggag
    13321 atcttcgaca tgctgctggc tacatcatct cggttccgca tgatgaatct gcagggagag
    13381 gagtttgtgt gcctcaaatc tattattttg cttaattctg gagtgtacac atttctgtcc
    13441 agcaccctga agtctctgga agagaaggac catatccacc gagtcctgga caagatcaca
    13501 gacactttga tccacctgat ggccaaggca ggcctgaccc tgcagcagca gcaccagcgg
    13561 ctggcccagc tcctcctcat cctctcccac atcaggcaca tgagtaacaa aggcatggag
    13621 catctgtaca gcatgaagtg caagaacgtg gtgcccctct atgacctgct gctggaggcg
    13681 gcggacgccc accgcctaca tgcgcccact agccgtggag gggcatccgt ggaggagacg
    13741 gaccaaagcc acttggccac tgcgggctct acttcatcgc attccttgca aaagtattac
    13801 atcacggggg aggcagaggg tttccctgcc acagcttgat gaagatctga gctcttaatt
    13861 aatgatcata atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc
    13921 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
    13981 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    14041 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    14101 gatccgttta aactaacttt aaataattgg cattatttaa agttacatat ggcggccgcc
    14161 ctaaggctcg acctcgaaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
    14221 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
    14281 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
    14341 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
    14401 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
    14461 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
    14521 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
    14581 gggcggggcg ggcgcccgaa ggtcttccgg aggcccggca ttctgcacgc ttcaaaagcg
    14641 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcatccatct
    14701 agatctcgag cagctgaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
    14761 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
    14821 cacgcgccac accgtcgatc cagatcgcca catcgagcgg gtcaccgagc tgcaagaact
    14881 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
    14941 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
    15001 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
    15061 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
    15121 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
    15181 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
    15241 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
    15301 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
    15361 gagcgcacga ccccatgcat cgatgatatc agatccccgg gatgcagaaa ttgatgatct
    15421 attaaacaat aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag
    15481 ggtgagaaca gagtacctac attttgaatg gaaggattgg agctacgggg gtgggggtgg
    15541 ggtgggatta gataaatgcn tgctctttac tgaaggctct ttactattgc tttatgataa
    15601 tgtttcatag ttggatatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt
    15661 cctcccactc atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga
    15721 ttcccacttt gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag
    15781 aacgagatca gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt
    15841 ctaattccat cagaagctgg tcgagtaact ttaaataatt ggcattattt aaagttaggc
    15901 gcgccataac ttcgtatagc atacattata cgaagttatg tcgagatatt taaatattag
    15961 acccagcttt cttgtacaaa gtggttgata tctctatagt cgcagtaggc ggaacggatt
    16021 gagagagatc agccccaaga gctgagaagt tcctattaca ttctcatcat ctattgtaga
    16081 aagatttcaa ataaggactc ttgtggggtt tttctttact ttcaccctcc cttcccaagt
    16141 ccctgtctac tgcctatatg ctaaagccaa cttccagaat gttctctgtc cctcctctat
    16201 tcacacattc cctctcccac cttctctagt cagtcaggac acctctctac caagcaaacc
    16261 tcgacaccca gcgataagta acttcctgta atctagttgc ctctcacagc actaatttgg
    16321 catttgtgaa acttccctag agtttcccct tcaatcccct tcccttttct tcttttccca
    16381 gaatacaaag tctccatctc aggaaccagt gtagagttga cgtgccctct agacagtgac
    16441 gagaacttaa aatgggaaaa aaatggccaa gagctgcctc agaagcatga taagcacctg
    16501 gtgctccagg atttctcgga agtcgaggac agtggctact acgtctgcta cacaccagcc
    16561 tcaaataaaa acacgtactt gtacctgaaa gctcgaggta actcgggctc ctcccaaatc
    16621 agccttctca agaaccctat catctctcag ctgctcctgc actccacccc aggttctccg
    16681 gggccacaca ttcagtattt tctgaaaaat agactgcacg gtgtggcctg gagttgtcta
    16741 ggtaattcca ttggtaccag gtgtacaaca gcaacttccc acagatgaag gcttgggtca
    16801 cctgccttgt aacgtaccca gaatcaaggc ttactactat catgagttga tggggtcact
    16861 tattcagaga cccattttat tacaaggaca tccagagaga ctcagggcca ctacctaaaa
    16921 gtcccaaatg ctggcatcac aatagacaat aggagctcca tctgtcgggt actgtatttg
    16981 tctttgggtt gaggttttgc tttggtgttt gtttgttgga ttaggttttt ttcttttgta
    17041 atagtcctgg atatcctgaa actcactcta tagaccaggc tggtctcaaa ctctcagaga
    17101 tctgcctcca gagtgctggg ttcaaaggca agtacctcca gcctaagtac tgtatttgta
    17161 atccctacac atgcctcagc tacccaaggg cctggggaac actgggtaag ccttatactt
    17221 ctgagctcca ccccgaaggc ccatacgatg ggatcctttc ctgctatccc cacaaaagtc
    17281 atatagcttt agcccatttt tattcccatt ttgttgatat gaaagcagag gctttgtggc
    17341 ctgaaaaaat ccccacaact aatgagtaat agagctgaga caccagagcc aataatcata
    17401 atcacccata ttgcttctta ctatcatcct cattggacac cactgcagga aagactctgg
    17461 tgctggctat catgcaggtg tctaaggaag acttgtcttc atttgtttct tcctcttctc
    17521 ccacatcctg gtatttatta tctttttttg ggtcatttct ctccctcccc cacccctgtg
    17581 tcctgcagtg tgtgagtact gtgtggaggt ggacctgaca gcagtagcca taatcatcat
    17641 tgttgacatc tgtatcactc tgggcttgct gatggtcatt tattactgga gcaagaatag
    17701 gaaggccaag gccaagcctg tgacccgagg aaccggtgct ggtagcaggc ccagaggtaa
    17761 aaccatcgaa ctgggtcagg agacatctag cctggaccag gatgggcacc aagtctacag
    17821 cagattccaa atagattctt aagcacattc tagatcccta tcaaaggtcc cccattcaaa
    17881 atcattcctc tcctcctacc ctgaaaaccc ctgaagacca accctgacca accaagccag
    17941 tgggcttcct ccaacgcctg gagtgctcag tagtaaacag attcagtccc caagctcggg
    18001 attttagctg gtacctcctc ctctcccttc cccctgcaca gataatggaa gagtcacacg
    18061 tgatgagaaa tcgtgggagg ccttagggat tgggtgaagg aaagaaaagg aagagtagct
    18121 gggtgggaaa aggggattag cagaaagaac agagaggatt gccctccctt gggttggatc
    18181 tcctagcagc tatctttcct gcaagactag cgttcagcat ggatacagca ctttccccct
    18241 ctgtcctctg cagggcaaaa caaggagcgg ccaccacctg ttcccaaccc agactatgag
    18301 gtaaccggat atggtgggtg gcatgttgga gggggctcct cctagagaga ccagcaggga
    18361 ctgtccatct tatctgccag acacttcaag ctgctctctc agggctctca ggacaggctc
    18421 cccatgtgcc tgccctctct gccttcttca tgttataatg ggactatctc aaaggctgtt
    18481 tggagatggc gcaacgcaat taatgataac ttcgtatagc atacattata cgaagttatg
    18541 gtctgagctc gccatcagtt cacatttgca aatgttaacc agatagatag gaaaaggatg
    18601 gtgtcccact agcatgccct aggacagtca gacagttgtt aggacggcgc tgtgctggat
    18661 taatgatagc ccctagtttc cctccgtcat gacacacaca agcacatgca cgtgcatgca
    18721 gccaggcacc acagcccctc tcccaccagg ctgccatttg gtttggcagt tgaagagtta
    18781 ctgcctggca ccagggcttc ccttggtgtg gctgtgcacc agcagtcctt gaagattttc
    18841 tatcacattg atgaaccgta caaaatttag agcaccagga ctgaggtatt taaagatcac
    18901 agtaactcca gtgacccttg acaaaagtca gtgtttgcag cctccatagc tgagttaggg
    18961 aagctgaggc tgccagaagg aagagactgc cttaagtgtg ccaagttaca attgggctcc
    19021 tcctgactcc aagcccagag gtctcacctt gcgctgcagc caaccaaagc tccaatgcct
    19081 tattctccct tcatatctcc ttcctcccac catcctaatc acccttcccg ttccaccctc
    19141 agcccatccg caaaggccag cgggacctgt attctggcct gaatcagaga gcagtctgac
    19201 agataggaga gacatcgcct tctgtggacc cagatccagc cctccgagca ccctgctact
    19261 ccttgttctc tggacagact gcagactcca cagcttgctc ttcagcctcc tggtgaacac
    19321 gtgtcctaga accttgctct cctgcctcct ctgctagtag ccagtgctgg gacattgctg
    19381 actcaacagc ctttgaaaga atcaggctgc tcagattgtc tgccagccac cttgtgggga
    19441 tacttttttc agccgccctg ctgccagctc cccgctgcct caccagtgtc ctctctgcct
    19501 cagttccttt cctctcctaa ttggccctca tagctaagcc ctttcctaca gctttctgtt
    19561 ttttcttttt ctttcttttt aggttttctt tcttttcttt tttattcctt tttatttaat
    19621 cttttttttt taaacactcc agattttatt cccttcccgg cccatcctcc gactgttaca
    19681 caccccatac ctcctccctg cccccttgtc tctacgagaa tgtcccccac ccccatcccc
    19741 cacctgcttt ctggtttttg gtttttggtt tgtttttttt ttttaaactc tgtgttttac
    19801 actcttctct gggatggatt ctgtaatcat tggcacaggt cctgccccat ttatagatcc
    19861 tggcccagcc cctgccacag gtgcctctcc agatttcccc ttagatcctc ggatggtcat
    19921 ctccatctcc atgaatacac cagccccctc tctgctaatg caaaaggcaa taaagtgtat
    19981 tggctgggaa agctatgtct cttttcttct ggaaggtcct tcaaagcagc ctgtgtccta
    20041 aggcagttca gaccttgccc aggcactcat tgcagtgctt cctgttagag gaggtggaca
    20101 ctagcaatcc ttccacatgt atcgcaacat caaaggtgca gagagtggtg gatgggtccc
    20161 tgtggagtgc ttggtcctta agatgattga gacacacaag gcacatgaac ggcaggtaga
    20221 gatacaagaa gagatgggat ggtgaggaag gaattggggc tgagaagctg gtttccatca
    20281 gctgccagga agtgctgctg aacagagcca gggaagacga gaaaagccgt gcgtgcgtgc
    20341 gtgcgtgcat gcatgcggtt ccctcctgcc ctggaggcag ctgtctgaag ccccaagcag
    20401 tagtggttat acagcaggat gtgggctgtg ctgggagtga ggatgagaga ggtgtggtgg
    20461 tagccacgga ccagacactg gaggctatcg atgagagaat gtggtccttg gtctcccaca
    20521 tactctgctt gcaggagaga ggaacccaga gcccaagcaa gctatcctag gccagctcaa
    20581 gccatcggac tctcttctcc acctttctgc cctcagacac atccctgctg acttaagggc
    20641 tatttgttat gtgactttag cttctgtgcc tagcagcggt ccacacactg tccatactct
    20701 gccttggatt tgtaactaca gttcctgcta gttggtctcc actaaactgt caccttttcc
    20761 tctctaggca tcgatgctta tgttaaaatg agtcttgatg attttgccta tcaccaaaca
    20821 ctaataagca ggtggcagat tttgtcctgc atatatttga tgtgtccagt caaggacatt
    20881 cgtagctcaa catgataaaa agcattaaca tttggtgttt actgagaatt taccatgctc
    20941 cagaagttgt tatagcccta tatgtgctgt ctcatttaat ccttatagag acgccacaaa
    21001 tatatgttgt catcttctat atagtgggct cagagaggta gcatcattta tccatgtgaa
    21061 gtgaaatgca gggctacaat tagcagctag agatgtctaa ttctggggca cttttttttt
    21121 tttttttgga tacagagtct cactatgtag ctctggctgt cctagaacta tgtatgtatg
    21181 aactaagcat gtagactaga aactatgtag accagactgt cctcaaactc acagagatcc
    21241 tcctgcctgt gcctctgctt cccgagtgcc aggattaaaa gtgtgcacta gtaatgctct
    21301 gcttctaact ccagcactct ggcacctacc cactatgacc gagaccatgg caatgttctt
    21361 cttcctacca tatgcctctg gaaaaaatgg aggagttgga gatggagcag aaacctagag
    21421 tctgttttct atatgggtcg gaggcaggat acaataggat gaaggcagat gccgtgtcaa
    21481 ggaaagtgag ggtcatgcaa aacacagctc ttactccaaa tagaatagca gttagtttat
    21541 tctggagcca gcaagatggc ttagcaggtg acagtgctta ccaccaagcc tgaagacccg
    21601 agtttgattc ccagaaccta cctggtagaa gaaaaccaac tttcataaat tatcctctga
    21661 ctgctaaccc caccaggcta gaagagcact accacaattt atgagccaaa tttgaagcaa
    21721 gctttttact ggccaagtct atggatactg gccagatcca tacgcactat ctatccccag
    21781 agctccagcc ctttataagg caggggctat aaaggcaaag cccataagac taccgacttc
    21841 ttcccacctt catctagtca gggacaagca tacaccctgc cacactttct gcctgtgaac
    21901 ctgctggcaa tgggtaagca agcacatcct gtgcagctgg ggcaacccag cttctttata
    21961 gaagcaaaaa ctcccggctt gttatcttcc atgatcaaca gcccccagca tcccaggaag
    22021 ttggctgttc ttgagcaagt ggggcttaca ggttagaggc attttgttta atagttagat
    22081 ctcttaagca cagtaactta tacttagaca taatgttagc ctcttgcgcg cgccggactg
    22141 gccagcagta acaacgctgc aacaggatcc ttctgcacat gtttattggg agagcttgat
    22201 tgtagaggcg aagagacccc gagcccagaa ctggtgctgc ttatataggc ctaggagatg
    22261 cgtgtctcac acccggattg gctatgcact acgcctcatt tgcatgttcc tcatctgatt
    22321 ggctactctc tctctctctg tacctcacag agcctcatta tcatacctca tttgcatgtc
    22381 tcacatctga ttggttatat tctcaaagct tcattatcat gcccgggcca ggcagtgtct
    22441 ttgcaaaaaa ctttactgca tatgtacaca ttggttgttt gtccaaactt atgcgtggtg
    22501 gccagcagta gtcagcgcca ctctgcaacg gcacatgtgg cttcccacat tagccatcat
    22561 atttctttct tgagctgtgt atcctgagtc aaatccttga ctctaaagtg ggtagcggct
    22621 caaattaaga tctaataacc atcagcttaa tggcacccag acagcaattt acatatatag
    22681 ttaagatatt aatgatgcaa ggttattagt aactagcaga aacagaaggg caaa
//