LOCUS       50415#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20938 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 50415
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000034756
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..8231
                     /note="5 arm"
     misc_feature    8259..8283
                     /note="B1 Gateway"
     misc_feature    8296..8343
                     /note="Frt"
     misc_feature    8344..8843
                     /note="mouse En2 intron"
     primer          complement(8412..8433)
                     /note="LRPCR LAR3_EN2"
     primer          complement(8308..8331)
                     /note="FRT3"
     primer          12509..12527
                     /note="seq primer NF"
     misc_feature    12569..12576
                     /note="RE sites"
     misc_feature    14369..14402
                     /note="loxP"
     misc_feature    complement(12577..12608)
                     /note="Rox"
     misc_feature    complement(14329..14360)
                     /note="Rox"
     misc_feature    12609..12630
                     /note="polylinker"
     CDS             13189..13785
                     /note="puromycin N-acetyl transferase"
     misc_feature    13304..13309
                     /note="Mutated RsrII site"
     intron          8844..9218
                     /note="mouse En2 intron"
     misc_feature    9216..9218
                     /note="Splice Acceptor"
     exon            9219..9272
                     /note="mouse En2 exon"
     primer          9119..9140
                     /note="seq primer FCHK2"
     misc_feature    9273..9395
                     /note="1Dleader"
     misc_feature    9396..9447
                     /note="F2A"
     misc_feature    9543..10259
                     /note="EGFP"
     misc_feature    9453..9542
                     /note="3X NLS"
     misc_feature    12325..12563
                     /note="SV40 polyadenylation site"
     misc_feature    11358..12302
                     /note="ERT2"
     promoter        12641..13152
                     /note="PGK promoter"
     misc_feature    13896..14328
                     /note="PGK pA"
     primer          12383..12406
                     /note="LRPCR RAF5"
     misc_feature    10269..10322
                     /note="T2A"
     misc_feature    10319..10319
                     /note="A to G to kill RsRII site"
     misc_feature    10323..11348
                     /note="Cre"
     misc_feature    9234..9273
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    9234..9249
                     /note="FCHK QC prefix region"
     misc_feature    9250..9267
                     /note="FCHK QC critical region"
     misc_feature    9268..9273
                     /note="FCHK QC suffix region"
     misc_feature    14425..14445
                     /note="B2 Gateway"
     misc_feature    8232..14475
                     /note="Synthetic Cassette"
     exon            15725..15819
                     /db_xref="ENSEMBL:ENSMUSE00000217973"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000217973"
     misc_feature    14476..15989
                     /note="Critical Region"
     misc_feature    16013..16046
                     /note="Downstream LoxP"
     misc_feature    15990..16069
                     /note="synthetic loxP region"
     exon            19326..19439
                     /db_xref="ENSEMBL:ENSMUSE00000217975"
                     /rank="5"
                     /note="ENSMUSE00000217975"
     misc_feature    16070..20938
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggattttaa aatagaacca cctaagattc ccagcaaggc tacaagacca
     4321 ctctcctcct atgcagcgga gaagggcaga acgtctttct tgtaagattg gggtgcctct
     4381 gtgggctgca ccccataaag aagtccttca taactcattg gcccagggcc atattgttcg
     4441 actagaaggg aaactggccc cactcttccc tttgcagagg cctctcgctt gaggctccgg
     4501 gtcactttgc aaaccagccc tcatctgaca gctgttctca gagtcagacc ctgttgagcc
     4561 ttcacagctg ctttaactcc tgagcacagc attcctgtct aagagcattg tctgcatagc
     4621 tgtgcaggaa ccaggtggct gcaaagcact ggggagggag tgcccagggc tctttcattg
     4681 ctggcttcat tggctgcctc tccctcctca gccctttctt ttttctactg aggggatcca
     4741 aaaaccagtt tcacccacga acgttggaag aaaccagatt ctaatctggc atctgcacgg
     4801 tgggctgagt gaagggtgag cctgcagggt tccgttgttg cagctgttca ggtttttcct
     4861 ttcctgttct ctgaggcaac tagggtacca gggcataaaa aggtgcccac attgtacaca
     4921 tgttactcca tttttgtttt tttgtttttt tgtttttatt tctttgtgtt ttcctactgg
     4981 ttctaggtgg aattttagaa acacactaga ttattctctg agaattagaa gggctttttt
     5041 ttttttttta attattttta agtcagggtc tcttgtattg cagaacttgc catgtagcca
     5101 cagatgactt taagtttcta atcctctggt tcccacctcc caagtgctag gattacaagg
     5161 atgtgccatg acacccagtt tatgtggcac caggttccaa actcaggact tacatgctag
     5221 acaaccattc tacctactga gttatacccc caagcctggg tttttagatg acaaatgcat
     5281 cccataccga taacaaagga gactaggcct taggtcctat ctaagaaacc tagccgaggt
     5341 gacatcagaa cagatgggaa tagaagtttg atctttgggc taaagtgctt tcttgggtat
     5401 tccgttgagc taggccatcc ttgaactggg tctgtgtccc tcaactatag cacacctgtc
     5461 agaggcctga tgctcctcag cccctcccct gggtatccaa ttcttgtctc agctttcagg
     5521 agtttctgtt gttcacctgg aaactgcact gttaacctca tgaaagctat tagcaaacac
     5581 ggcctgggct tctctctgcc ctgtcttttc ataggccaat agttgaatcc aggcagtggc
     5641 tgggtctggg gctgagaggc caagggaggg acttgcctcc catcattcag tcagtctttt
     5701 ttgtgtccta gcaacataag tcttacgatg gttcctgttt tatatctggg aaagccaggt
     5761 gcagagagag aagcattgaa ccccaaggtc gtatatgctt gagtctcagg tacccttcag
     5821 aatatcgcct cctctgctta cacagagacc tcctgtattc ggcactgtct catcccgggg
     5881 ccaaaatgcc aaacaaaagc gagttaaggg agagagggtt tactctggct catagtcgga
     5941 tagcccagcc atagtgcacc aggcatggag cagagcgagg aatactggtg ctcccctcac
     6001 agtgctccat tttccgtagt ccaggacctc cgccaatggg atggtgctgt ccatatttac
     6061 agtgggcttt cccacctcag tcaaccctac ctaggtaacc cttcactagt catgcataga
     6121 agtttgtctc ctaggtgatt tcagatcctg tcaagtcagt aataacaact atcacagtat
     6181 ccctgactcc ctggcccggt cctctctggc atactttagg cccttttgtg aagacagagg
     6241 atttcttcta agccaaagat atcaaagata gccagcaatc tgagctgtag tcctatcctc
     6301 ccgcccccaa ctagataagg caattttcct tttctttcca accagacagc tcccaccccg
     6361 aggcctcttg agccctcatc agacagacag aatctactct atgtgcagtc agcatgtctc
     6421 tggtgttgtg aatcagcatc ctgttccaga gggttcttgg ttccctgcca gagacagtgg
     6481 agacacgcat tctccatgga atgccgaagt ccagagcaga ccaaggattt cagcccgcag
     6541 agaggaagag agcaggggcg ggctttgtgg gtttggattc aatgcttgaa accttttaat
     6601 gcttgaaaaa tatggtgggc agctgagaag actcagcccc tcaaagtcac tgctggaact
     6661 cagctgtcag ttatgcaaag acctccctca ggtagactct gcttagaaca atgatggacc
     6721 tttttttttt ttaaccatgt cttcaattgg aatattgaaa atatgcaggt gattagacta
     6781 gccaaccatc ttcatccaag gacaacatgc ttaactgcga ccctgcaccc gtgtgtcact
     6841 acatcttggg tcttagttgg gcttaggcca cttgacttct gtaaaatttg agtctgttgg
     6901 ataagttttt acaaagatca cctgagtggg gcccagcagg taagaatgtt tgctgctctc
     6961 gcagaggact caagtttggt tcctagtacc caagttgagc tattcacagc cgcctgtaga
     7021 tgcagctgta ggggatctgt ggacgctttc gtctggccca cagtctcttt agatctcaga
     7081 tccttctacc tagccagtcc ttccttaaat ggggggaagt gagaagttgg aaagaaccca
     7141 gtgcacggat gcagagcctt acctgctaag cttctggagc taaccttgaa ggcatggccg
     7201 tcagtattga ttacaatgcc cacctggatg gctgagtgtt tgccccacct ccttcatctg
     7261 tagttagact gtggtggcca atggccgctt ggtccctgca gcttcaagct atgctggata
     7321 gttttccaca ggaacaaact gtttcatcca ctgggacatc aggtcaagga agcaatagtg
     7381 gtctaaagat aacatcaccg ggagagatgg ctcagaggtt aagagcactg tctcttcttc
     7441 caggggtccc aagttcaatt cccagcaact gcacggtggc tcacagccat ttgtaatgag
     7501 atctggtgtc ttctactgga ttgcagtcat acatgcaggc agaatgatgt atacataata
     7561 aataaatctt ttttttaaag ataatatcac caatgggtat tctggtgctt tagcataggt
     7621 tatgccttat gtgccagctc aaggcacata agcccagaga atgctgggct tcttgagaca
     7681 tgccagcaac tacctgtgac acagcactga tgcagatgag ggtttagagg gtttagggtc
     7741 cagagctgcc cacttgtaga ggatctctgt tcagtttctc tgcattgact gtcatcagca
     7801 tgatactgcg aaacctttga caaatctgct tctagatcca gccttctaca cagatccatc
     7861 catattcctt tgttggtttt gcttgtttgt ttttcgagac agggtttctt tgtatagtcc
     7921 atacagcttt aatctcagca ggcaggaggc aggaggcagg aggcaggagg caggaggcag
     7981 gaggcaggag gcaggaggca ggaggcagga ggcaggaggc aggaggcagg aggcaggagg
     8041 caggaggcag gaggcaggca gatttctgtg aatttgagac cagctgggtg aatccctgtc
     8101 tcaaaaccaa aaaccaccaa aaaactgaaa aaacaaacaa acaaaagcac aacaacaaca
     8161 aaaaatcctc aaaaaagaat aaaaataatt caccttgctt tattttaaat gaccacatac
     8221 atagcatagt aaaggcgcat aacgatacca cgatatcaac aagtttgtac aaaaaagcag
     8281 gctggcgccg gaaccgaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     8341 ttcgaaccct ttcccacacc accctccaca cttgccccaa acactgccaa ctatgtagga
     8401 ggaaggggtt gggactaaca gaagaacccg ttgtggggaa gctgttggga gggtcacttt
     8461 atgttcttgc ccaaggtcag ttgggtggcc tgcttctgat gaggtggtcc caaggtctgg
     8521 ggtagaaggt gagagggaca ggccaccaag gtcagccccc cccccctatc ccataggagc
     8581 caggtccctc tcctggacag gaagactgaa ggggagatgc cagagactca gtgaagcctg
     8641 gggtacccta ttggagtcct tcaaggaaac aaacttggcc tcaccaggcc tcagccttgg
     8701 ctcctcctgg gaactctact gcccttggga tccccttgta gttgtgggtt acataggaag
     8761 ggggacggga ttccccttga ctggctagcc tactcttttc ttcagtcttc tccatctcct
     8821 ctcacctgtc tctcgaccct ttccctagga tagacttgga aaaagataag gggagaaaac
     8881 aaatgcaaac gaggccagaa agattttggc tgggcattcc ttccgctagc ttttattggg
     8941 atcccctagt ttgtgatagg ccttttagct acatctgcca atccatctca ttttcacaca
     9001 cacacacacc actttccttc tggtcagtgg gcacatgtcc agcctcaagt ttatatcacc
     9061 acccccaatg cccaacactt gtatggcctt gggcgggtca tccccccccc cacccccagt
     9121 atctgcaacc tcaagctagc ttgggtgcgt tggttgtgga taagtagcta gactccagca
     9181 accagtaacc tctgcccttt ctcctccatg acaaccaggc tcccaggtcc cgaaaaccaa
     9241 agaagaagaa cgcagatcgc agatcgcaga tctctagagt caccgagttg ctttaccgga
     9301 tgaagagggc cgaaacatac tgtccaaggc ccttgctggc aatccaccca actgaagcca
     9361 gacacaaaca gaaaattgtg gcaccggtga aacagacttt gaattttgac cttctcaagt
     9421 tggcgggaga cgtcgagtcc aaccccggac cggtgcatgt ggaccccaag aaaaagcgga
     9481 aggtggaccc taaaaagaaa cgcaaagtgg atcctaagaa aaaaagaaag gtgcacgtgt
     9541 ccatggtgtc caagggcgag gaactgttca ccggcgtggt gcccatcctg gtggaactgg
     9601 atggcgacgt gaacggccac aagttcagcg tgtccggcga gggcgaaggc gacgccacat
     9661 acggaaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccttggccta
     9721 ccctcgtgac cacactgacc tacggcgtgc agtgcttcag cagatacccc gaccatatga
     9781 agcagcacga cttcttcaag agcgccatgc ccgagggcta cgtgcaggaa agaaccatct
     9841 tctttaagga cgacggcaac tacaagacca gggccgaagt gaagttcgag ggcgacaccc
     9901 tcgtgaacag aatcgagctg aagggcatcg acttcaaaga ggacggcaac atcctgggcc
     9961 acaagctgga gtacaactac aacagccaca acgtgtacat catggccgac aagcagaaaa
    10021 acggcatcaa agtgaacttc aagatccggc acaacatcga ggacggctcc gtgcagctgg
    10081 ccgaccacta ccagcagaac acccctatcg gcgacggccc tgtgctgctg cctgacaacc
    10141 actacctgag cacccagagc gccctgagca aggaccccaa cgagaagagg gaccacatgg
    10201 tgctgctgga attcgtgacc gccgctggca tcaccctggg catggacgag ctgtacaagc
    10261 ctgcaggcga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccgggc
    10321 cgtccaattt actgaccgta caccaaaatt tgcctgcatt accggtcgat gcaacgagtg
    10381 atgaggttcg caagaacctg atggacatgt tcagggatcg ccaggcgttt tctgagcata
    10441 cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag ttgaataacc
    10501 ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta tcttctatat cttcaggcgc
    10561 gcggtctggc agtaaaaact atccagcaac atttgggcca gctaaacatg cttcatcgtc
    10621 ggtctgggct gccacgacca agtgacagca atgctgtttc actggttatg cggcggatcc
    10681 gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg
    10741 atttcgacca ggttcgttca ctcatggaaa atagcgaccg ctgccaggat ataaggaatc
    10801 tggcatttct ggggattgct tataacaccc tgttacgtat agccgaaatt gccaggatca
    10861 gggttaaaga tatctcacgt actgacggtg ggagaatgtt aatccatatt ggcagaacga
    10921 aaacgctggt tagcaccgca ggtgtagaga aggcacttag cctgggggta actaaactgg
    10981 tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc gaataactac ctgttttgcc
    11041 gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag ccagctatca actcgcgccc
    11101 tggaagggat ttttgaagca actcatcgat tgatttacgg cgctaaggat gactctggtc
    11161 agagatacct ggcctggtct ggacacagtg cccgtgtcgg agccgcgcga gatatggccc
    11221 gcgctggagt ttcaataccg gagatcatgc aagctggtgg ctggaccaat gtaaatattg
    11281 tcatgaacta tatccgtaac ctggatagtg aaacaggggc aatggtgcgc ctgctggaag
    11341 atggcgatct cgagccatct gctggagaca tgagagctgc caacctttgg ccaagcccgc
    11401 tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg
    11461 tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac
    11521 ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc
    11581 acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg
    11641 tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca
    11701 tggagcaccc agtgaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa
    11761 aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc
    11821 gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt
    11881 ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc
    11941 accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga
    12001 ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc
    12061 acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc
    12121 tctatgacct gctgctggag gcggcggacg cccaccgcct acatgcgccc actagccgtg
    12181 gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat
    12241 cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagctt
    12301 gatgaagatc tgagctctta attaatgatc ataatcagcc atatcacatc tgtagaggtt
    12361 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
    12421 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    12481 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    12541 atcaatgtat cttatcatgt ctggatccgt ttaaactaac tttaaataat tggcattatt
    12601 taaagttaca tatggcggcc gccctaaggc tcgacctcga aattctaccg ggtaggggag
    12661 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
    12721 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
    12781 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
    12841 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
    12901 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
    12961 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
    13021 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcttc cggaggcccg
    13081 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
    13141 ggcctttcga cctgcatcca tctagatctc gagcagctga agcttaccat gaccgagtac
    13201 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
    13261 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccagatcg ccacatcgag
    13321 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
    13381 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
    13441 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
    13501 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
    13561 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
    13621 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
    13681 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
    13741 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac
    13801 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgatgat atcagatccc
    13861 cgggatgcag aaattgatga tctattaaac aataaagatg tccactaaaa tggaagtttt
    13921 tcctgtcata ctttgttaag aagggtgaga acagagtacc tacattttga atggaaggat
    13981 tggagctacg ggggtggggg tggggtggga ttagataaat gcntgctctt tactgaaggc
    14041 tctttactat tgctttatga taatgtttca tagttggata tcataattta aacaagcaaa
    14101 accaaattaa gggccagctc attcctccca ctcatgatct atagatctat agatctctcg
    14161 tgggatcatt gtttttctct tgattcccac tttgtggttc taagtactgt ggtttccaaa
    14221 tgtgtcagtt tcatagcctg aagaacgaga tcagcagcct ctgttccaca tacacttcat
    14281 tctcagtatt gttttgccaa gttctaattc catcagaagc tggtcgagta actttaaata
    14341 attggcatta tttaaagtta ggcgcgccat aacttcgtat agcatacatt atacgaagtt
    14401 atgtcgagat atttaaatat tagacccagc tttcttgtac aaagtggttg atatctctat
    14461 agtcgcagta ggcggtatcc tcaaacccac ctcagtgtct agcaataggg tattcacact
    14521 gtattcacac tgtattcaca ctgactactc taaaatctaa gtggagtgct agacatttat
    14581 tagtacgtga ccccacttgg aaatgagtac atgaatgttt taaagcatct ttctttttat
    14641 acccggaaat tatcacgttg taaatgcgcg tgaagcatca ggatgcttcc actgtccaac
    14701 tgttcattgt tctgccgccc aaacatcata gttctgagct ccaagtgctt tgggatgcct
    14761 ctgactttct gtaagacaag ttgggtgcag tctgtaagtg accttttgtg cttagggact
    14821 ctggaattgc ccaccagtca tggcaaaggc ttttcctttt attgagcaaa gttcatagtc
    14881 agacagcgga aagctctctt gctgcctgaa tcctcaaaac tgagctttgc tgagttaaat
    14941 gacatgtgag gaatatttct gatataaaaa ttggaaactt tcagatacct cgaaccttgc
    15001 ttatctttgt aaatacacat gttccaatta aaataggtac tgaaacattt gggtggcact
    15061 tcaaaactcc caatttagat ttaatttaga ttaaagcact tattcttttt taatacagtt
    15121 gtaaaattga ttattaaagt cacctattga agattgaaag cacaggatgt atactttcct
    15181 tgtgagacaa tctcatcttc aataagtgtg gttctattaa tcaattagct attaaaatat
    15241 aactgccata ttaagcctga ctcatttgtc atgacagctg gtataattat aaaaattata
    15301 cctagccatt tatacagcca taatcttgca ataaatataa ataattgtct ctattaattc
    15361 attttgaagg ttctgtctat taattctcca gttaagtggc aatttgggga ccttcattta
    15421 aaatctttgt tgttcgttcc atctgactgc cagagttcca cacgtgtacc tcccactgtt
    15481 gattttggaa accaatttat gaataaaata gctttttaag tgcaaatatt taaagggttg
    15541 aggcccaagg tccaaggctt tctggggaag aaggtaatga gggtgcctgt gtttggtcag
    15601 gaagagggat gagctagtcg tgacattcta gtattgggtt ggggagagag tatgtggatt
    15661 tcactgtgtg aacgtctcct aaagcgtgtg gtggggtaac tgttcataac ttgattctcc
    15721 acaggagtgg atgaggtcac cattgtcaac atcctgacaa accgcagcaa tgtgcagagg
    15781 caggacattg ccttcgccta tcagagaagg accaaaaagg tactggaacc ttagtgtgga
    15841 gggcaggcct tgttgagtta atgttctcag atattgcctg ttctgttctt aattaatatg
    15901 tgtgtgtgtg tgtcccttgt ttgtgttgcc ctgcataccg tagggtggca ggccctaaag
    15961 cttccagatg attcccctgt cttcaatttg agatggcgca acgcaattaa tgataacttc
    16021 gtatagcata cattatacga agttatggtc tgagctcgcc atcagttcaa ttctccaccc
    16081 ctaccccgtc caacattact ttttcttttt gtgagttgtg gatcttcttt aaccaaaagg
    16141 atccttctag aactcttgga tgtccaaggt ttccctctta tatccttgag agggttgctg
    16201 ctttcatagg tacagccgtg ggaggtagaa tggaaaggtg acatcctgga acacacgagg
    16261 gaggcattct ggaatagacg acaaggaaga gaagcaatgg tcccacgggt tatgtgctga
    16321 ctctgtcatt gctgtgctta ggtagcaact cacttcctct catggcttct ctggcttctt
    16381 cgtcagatgt tgtgaatgta atttcagaat ggtttcctcc actgggcgtg agtcctaacg
    16441 tgtgcttctc atttacactg aggggctggg gagacagctc tgttggtaaa gtgcttgtca
    16501 cacaagcatg agtatctgag ttcaatcttc agcatccacg ttaaaagagc taaatacccc
    16561 agtgctaggg acgctagata ggattcctgg ggctccccag acagtcaatt tagctgattc
    16621 agagagctct aaatctcaca cacacacaca cacacacaca cacacacaca cacacacagc
    16681 ttctttatca gattcatcgt ggctgaagtg ctgtggtcca gcctgctatc ttcaataggc
    16741 ttcgctccta gtgtttatgg agttttctgt gtgtgttttc cctttgctcg tccactcgtg
    16801 gctaaagcag gccccgtctt gtccacctta gtttcccaca gcactaggac attttacctt
    16861 taaggatatg ctacttaatc tctaacgctt tgctattaaa agcttttgac ttatattcgg
    16921 acagtgccat cagggtgacc tcatgcggct cccgcctgac ttgaggcttt cactccccgc
    16981 tacagtagca ttgacctgaa agaatgccag gagaaactgt gtgtccctgg agttggcgtg
    17041 accaccaggt cctgggagtt ccaggtcaag cctggctgaa gctctgtcct ccgtgctcca
    17101 aagcagttgg gaaaggacgt aaatgggaaa gcagctagct cccttcatcc cacatgcatt
    17161 acaattctcc tttcacggga tgacgtgtgt taatcttctg cagtgaggct cagaagggag
    17221 tgttttatgt ctctggctgt cccaaaggat ctgagagctg caagagccaa gttcccgttg
    17281 gaacaggaga tccactattc tggactgaag gaaataactg ctcattcagt gtggccttgg
    17341 aactgtgtgt gcacactgag cttggcttca tgttaggtag ctggagaacg cagatcctgc
    17401 agagcctgtt ggagtaaaca cattattaga atcatgagaa gggtcagcaa acacacaggc
    17461 attagcgcgt gctaaggaca gcccactgcc ctgatgatac ttaatggtcc agggtgatta
    17521 tctgtcacag gaagatgccg caactgcctg aggtaatttt ctaaaaggac gtcctagtat
    17581 tgttttaggt gcactgtttg tcaagactgg tctgcagcag tggttcacag tctgtgggtc
    17641 atgatctctt tcaggggttg agcaaccctt tcacaggact cgcctaagac caccggaaaa
    17701 cagatgttta cctgatgact cagagtagca gcaaacacac agttatgaag tagcaacaaa
    17761 aatacttttg tggctgggat caccgtggcc tttctgacca ggacaaagaa ccctattaaa
    17821 gggttgcagc actaggaagg ttgcgaacca ctggtctaga gggacagagg gaccctgccc
    17881 aggtggtgag ctgctgcaga tactgtcaca gctagtaaaa gagggacaca gggaggaagt
    17941 ttgcatttta tgctttgtcc cttcaaacta gtgctagaca caaggtttct ctatgcagag
    18001 aacagttgtt gttctattga tcccttctgg gatttttatt tgtgctgagt cctatactgg
    18061 gcattctgag caattcaagt caaagtggaa aaatccagta ggtgccccag atgtcccctg
    18121 aggtgagagc agagatgggg gtgggggcgg gagggggagt gtcaaggaca gaccaagttg
    18181 ggagtcactt aacctcaggg ttccaatttg gaaagctggt gttataattg gtatgtgttg
    18241 ttttagaaag accttcctac ctcccatgca tggttagggc atcagcagcc agaaggagga
    18301 ggtgaatggg ctgggcctaa tgcttgttcg catccatgcc aggtctgact ttcttgacac
    18361 ttgctgtatg ttacctttag aggcttgtgg agcctcagaa attgttggcc agccagccta
    18421 cagggccaag ttccaggcca acaagagacc ttatctcaat cagaaagtgg agggcacctg
    18481 aggaatgaca actaagcttg ttctgtgacc ttaacccgca tatgctccta cacacacaca
    18541 cacacacaca cacatgcaac acgcacaccc cacataaaaa taaatgcagt tcagttcagt
    18601 gcagctgtgg cctagtccat tcccatggct ggtgagtatc cagtcgccga ggaaatatct
    18661 ttaagagcca ccctgaatta aggatatctg tctacatatc tgagtgctgc ctcagacctc
    18721 cgactttatt aaaattgtgt gttcatgatg atagtggcgt gatgatgaca ctgtccaaca
    18781 ggaagttgaa agggactctg aggggctgga gttcacatca tattcatcac cgccccaacc
    18841 tggaggagat gggagatggc tcagggcgag gtgcggtgtt ttacataggg aaagctcagc
    18901 accctccccc ccacccccgg cctcgctagc acagctagct ccaggctaca aggtcccacc
    18961 ctagagcagc atcacaaacg ccttccctgg gacctctata gacctgcagg actccactgg
    19021 tctttctacc tcacttcttt tcagcttgac ttggaaatct ccctgagact ccactcttag
    19081 ctctcctctc aggagctatc agctacagct acctagtgat cagcctgggt gttagtggat
    19141 tcccttacca tgtggtttct gccctcctgg tctgatgtag gggacgagga agttctcccc
    19201 aaacggcaga gtggtcaaag actcacacac agcctgcttt tcagagagag ccatcttatt
    19261 tctgtctcgc tggttccaga gtggacgttg gttccaagca gctctgagac atctcccttt
    19321 tacaggagct cccgtcagcg ctgaagtcag ccttatctgg ccacctggag acggtgattt
    19381 tgggcctatt gaagacacct gcccagtatg atgcttcgga actaaaagct tccatgaagg
    19441 taaacacgtg tggggtgtca tccgtcaacc tgcccattag cgtctgtgaa ttacagcctt
    19501 ttgagtgtaa ctaggtgatt gtcagttgtg ttggagtctt atttgaagtc atttcccccc
    19561 aatgctataa gttgtcagga tttgaaccaa aaatatttca atctctctct tttttaacga
    19621 cggcaaagaa atttcctgat gcttagcagc ttagtgatgg ttagcagctg ctgagactcg
    19681 gcacctgggc gattggaatg cctcttggga aaactgcttc cttgattaca cttccttttt
    19741 aaggaggata tgtgtggtat taaggagaaa gaaaaacagc ttttgttcag taaagcttgc
    19801 agattctgcc catgccctcc tggatgctgc ttcctctggc ttccccctgc ccccccccaa
    19861 tccgttgctg ggccaaaatt tggatttggt ccaaaaatca gcaatgcagt gcaggtgagg
    19921 gtcgatatca gggtgggact ggcacctgaa tccaatggga agtttctgca ggacataata
    19981 ttgttatcta agttttgatt ttgcaaagct attttgaaca tgggaattga ggttaaatta
    20041 gtaaattcta gtttagaaac gtccttggat tttgtgctca cctaagctac ccaatattac
    20101 tcaaatgtaa gagatagaca aatgtctgcc acagcaaagc tcagggcccc ttctctgtgt
    20161 acttgtgaca tgttggataa tctgaggctg ccctctgctg gtactttttg gtaaggcatc
    20221 ttcaatgagg ggaagctgta tgtcaccctg ttgggttgtc atcctttctg gagaacacac
    20281 taggaatgag gccccttcac acatagagta ccaccagaac cctggaactg atagcccaga
    20341 gccactgtag gatttgtcaa atctctctct gtctcttttt tagttcatca gcatagctca
    20401 agtggcatcc taggcaggct gtactgcaga ggaggtacca gtcagtgccg tgacacacca
    20461 ggcacagggt gcaggctaat aatcctagca ctccagaagt ggaggcaggg ggatcaaaag
    20521 ctcaaggaag tttaaggcca gggtgggata tctgagacct tgtctagaga gagagagaga
    20581 gagagagaga gagagagaga agaagaagaa gaaggagaag gagaaggaga aggagaagga
    20641 gaaggagaag gagaaggaga aggagaagga gaagaagaag aagaagaaga agaagaagaa
    20701 gaagaagaaa gaagaaaagg agagtgtact ttcatgccaa caacagcttt gatgatagta
    20761 gcattcctga gattgccaag cctttgggcc attggggtca tgtgtaagcc aattttttcc
    20821 caggtttctt taactatcaa cacttggatc agttcatctt atttttgtca agaatccact
    20881 tagttgagat ttatgtgatt tcactggtat ctatggtcgg gtattattga cttaaagc
//