LOCUS       240679#pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAroxbActNeopArox#L3L4_pD223_DTA_T_spec        22581 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2GT0_LF2A_nEGFPOT2A_CreERT2_pAroxbActNeopArox
COMMENT     design_id: 240679
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7371..7495
                     /db_xref="ENSEMBL:ENSMUSE00000188318"
                     /rank="1"
                     /note="ENSMUSE00000188318"
     exon            8203..8260
                     /db_xref="ENSEMBL:ENSMUSE00000188334"
                     /rank="2"
                     /note="ENSMUSE00000188334"
     exon            9182..9314
                     /db_xref="ENSEMBL:ENSMUSE00000188329"
                     /rank="3"
                     /note="ENSMUSE00000188329"
     misc_feature    4275..9999
                     /note="5 arm"
     misc_feature    10027..10051
                     /note="B1 Gateway"
     misc_recomb     16163..16194
                     /note="rox"
     misc_feature    16157..16161
                     /note="for bActNeo"
     insert          14077..16156
                     /source="pL1L2_GTIRES_BetactP FLFL MapVerified"
     misc_feature    14380..14900
                     /note="human beta actin promoter"
     misc_feature    14776..14795
                     /note="betact5"
     misc_feature    14926..15911
                     /note="neomycin resistance "
     misc_feature    15095..15114
                     /note="neoPstI_5"
     misc_feature    15432..15450
                     /note="neoSphI_5"
     misc_feature    15725..15744
                     /note="neoBstB1_5"
     misc_feature    15976..15999
                     /note="RAF5"
     misc_feature    15918..16161
                     /note="SV40pA"
     misc_feature    15570..15570
                     /note="G to C conservative mutation to kill RsrII site"
     misc_feature    15158..15740
                     /note="synthetic fragment mutates RsRII"
     misc_feature    13112..14056
                     /note="ERT2"
     misc_feature    14080..14322
                     /vntifkey="21"
                     /dnas_title="SV40 pA"
                     /label=SV40 pA
     primer          14137..14160
                     /vntifkey="27"
                     /dnas_title="LRPCR RAF5"
                     /label=LRPCR RAF5
     primer          14263..14281
                     /vntifkey="27"
                     /dnas_title="seq primer NF"
                     /label=seq primer NF
     misc_feature    14323..14330
                     /vntifkey="21"
                     /dnas_title="RE sites"
                     /label=RE sites
     misc_feature    complement(14331..14362)
                     /vntifkey="21"
                     /dnas_title="Rox"
                     /label=Rox
     misc_feature    14363..14370
                     /vntifkey="21"
                     /dnas_title="polylinker"
                     /label=polylinker
     misc_feature    10064..10111
                     /vntifkey="21"
                     /dnas_title="Frt"
                     /label=Frt
                     /note="Frt"
     primer          complement(10076..10099)
                     /vntifkey="27"
                     /dnas_title="FRT3_oligo"
                     /label=FRT3_oligo
                     /note="FRT3"
     intron          10112..10986
                     /vntifkey="21"
                     /dnas_title="mouse En2 intron"
                     /label=mouse En2 intron
                     /note="mouse En2 intron"
     primer          complement(10180..10201)
                     /vntifkey="27"
                     /dnas_title="LRPCR LAR3_EN2"
                     /label=LRPCR LAR3_EN2
                     /note="LRPCR LAR3_EN2"
     misc_feature    11297..12013
                     /note="EGFP"
     misc_feature    11207..11296
                     /note="3X NLS"
     misc_feature    12077..13102
                     /note="Cre"
     misc_feature    12023..12076
                     /note="self-cleaving' 2A peptide"
     misc_feature    12073..12073
                     /note=" A to G to kill RsRII site"
     exon            10987..11026
                     /vntifkey="61"
                     /dnas_title="mouse En2 exon"
                     /label=mouse En2 exon
                     /note="mouse En2 exon"
     primer          10887..10908
                     /vntifkey="27"
                     /dnas_title="seq primer FCHK2"
                     /label=seq primer FCHK2
                     /note="seq primer FCHK2"
     misc_feature    11027..11149
                     /note="1Dleader"
     misc_feature    11150..11201
                     /note="F2A"
     misc_feature    11022..11027
                     /note="FCHK QC suffix region"
     misc_feature    11002..11017
                     /note="FCHK QC prefix region"
     misc_feature    11018..11021
                     /note="FCHK QC critical region"
     misc_feature    16207..16227
                     /note="B2 Gateway"
     misc_feature    10000..16257
                     /note="Synthetic Cassette"
     exon            16649..16860
                     /db_xref="ENSEMBL:ENSMUSE00000188327"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000188327"
     exon            17518..17650
                     /db_xref="ENSEMBL:ENSMUSE00000188325"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 2 ENSMUSE00000188325"
     exon            18539..18636
                     /db_xref="ENSEMBL:ENSMUSE00000188335"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 3 ENSMUSE00000188335"
     misc_feature    16258..18795
                     /note="Critical Region"
     misc_feature    18819..18852
                     /note="Downstream LoxP"
     misc_feature    18796..18875
                     /note="synthetic loxP region"
     exon            19966..20095
                     /db_xref="ENSEMBL:ENSMUSE00000188330"
                     /rank="7"
                     /note="ENSMUSE00000188330"
     exon            21103..21317
                     /db_xref="ENSEMBL:ENSMUSE00000188324"
                     /rank="8"
                     /note="ENSMUSE00000188324"
     exon            22124..22256
                     /db_xref="ENSEMBL:ENSMUSE00000188317"
                     /rank="9"
                     /note="ENSMUSE00000188317"
     misc_feature    18876..22581
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaaattgc ttattatgtt tcagttgttg agtttgatgc ttagtggtct
     4321 tccacttccc attttttata aagcatttct ggccccgcag aataggaggg agctgcatat
     4381 gcccactcaa caataggaaa agtgtggaga aaagacttcg tgtccccatt tttcttcaaa
     4441 attaaaccaa attttctaca ttttacagtg gatagaggtg aagtcactat ttctagaaaa
     4501 tctgtggtga aattttgaag acattattag atacagctgt ttatttttct ggaccggttt
     4561 tccttttttt ttctattttt tgagacaggg tttctctgta tagccctggc tgtcctggaa
     4621 ctcactttgt agaccaggtt ggcctcgaac tcagaaatcc acctgcctct gcctccccag
     4681 tgctgggact aaaggcgtgc gccaccacgc ctggcttctg attttacttt taagaggaga
     4741 tccagaagta ctgtaggatc atgggggaag aagcatgcag gctggaccaa ggttgtcaca
     4801 tggccaggat ggcttgaaag aagttagaga cgttccagaa ataggaagga gagagagagc
     4861 tgggaaaact gggaaaacca tcctatcaaa aacgaatgca aaagaaataa gaaaagcaaa
     4921 acagaaggaa acaacaaaga ggtgatagta taaggagaaa acaagggatt gaaaaaattg
     4981 aagctcaggt ttaattccca gtcacatttg tattcagcag gagtgtatgt gtgagagtgt
     5041 gtgtgagtat gtatgtgtgt ttgagtgtgt gtgtgtgtgt gtgtgtgagt gtgtgtgttg
     5101 ttgttgttcc cttctagatg acgagcacag gaccatactt tccaaacaac aaatcatctc
     5161 agctactctt catttttaaa gtcctcccac aagcacgaga aaacactcaa agatcacttg
     5221 agctattttc tctttattca gaaactaaac ctttagttta acttttgcat ttatgctcct
     5281 gccaaactct tttgatgact gactaaccat cgagtttaaa agcttgaaaa acaggatgtc
     5341 tagaagtaac taataattat gtggcttcat tcctctcagt atagagtcta catataggac
     5401 gagtgccagg agtttcagaa ggaagagtat aaattcttac tcaccagatg caactgctca
     5461 agtgtttctc ttcagttgaa atgatctgtg agcacggaga ctgtgtcaag aattaagcac
     5521 tttaattctc ggctgctaaa gattaattag aacatgcccg agtaaggcat tgagttagta
     5581 ctgctaagga gaggctcagt tgttattaat ttatgtctta aaagtattgg agcttcatca
     5641 gtcactaaac cagctcaagc gcctacttca ttctgatgct tttctctcca ggctttggct
     5701 gcatagatga tttttttttt tttttttact taattgtcaa acaaaagtat atatagtata
     5761 catagtacat gtgagagtgc taaaagcctg agttaataac acaatattat acttaaacac
     5821 aatattacat ataactatat agtacttgtg agttaataat acaaatacat tcaatgactt
     5881 aagcattaca tacttgttta tgtttatcaa gacccacacc aggacaggac taggcatgac
     5941 tactgtcttc aaataaatag tgactactct ttctaggaag tttaaagagg aattgatcca
     6001 agaaagaatt atctcttctg tttagtttct ttttttcttt tccaggatgg ggttttggct
     6061 taattctgcc acccaccctc accctcagta gaagttattc catgggcaga ctttatctat
     6121 ctatctatct atctatctat ctatctatct atctatctat ctatctatct atctacctac
     6181 ctacctccct atccatctat catctatcta tctatctatc tatctatcta tctatctatc
     6241 tatctatcta tcatctatct atctatctat cattctgttc atctgtgtag aggctagtct
     6301 gttattccat ttcaggactc ttttgagtga taaaacaagc aaggaaacac ttttcccttc
     6361 tccttcattt tgccttgact cctgtggttg attacagaat ttgtacacct ttctgaagat
     6421 gaggacacaa gatgaggtca taagacatag atttctttca ttgtataacc agatataaag
     6481 aagaatgctt ctcctcctcc tcctcttcct cctcctcctc tcttcctcct tttccttctt
     6541 ctcctccttc ctcttctcct tcttgtcact gaatagtccc tgtatattta ggaaccatgg
     6601 ggttgatttg gatgtagtct tgactgactc ataagtgaga taactggtct gctactaatt
     6661 attatcctgt ggtgttagag gaagtatgtt acgatggcaa agaccatcat cactgtcatt
     6721 ggagtgcctg ttctagtgtc tcctctaaag caccccgaga aagaggttca tgttagatga
     6781 aaacattcta ctccagaccc gtttcacaca tagcctgagc atggggtggg gacacaagac
     6841 ttctgaaagt cctctgcact tgttttttat tgctctggct tcttctcagt tcacatttga
     6901 gacactgtgt gctttatgga agattctaac tttaaaaaga tgagaaagac ttggaagccc
     6961 tataatgaga cccacccctc cctcatacac attcttcatt acctgtacat aaaagcatat
     7021 ttgggatgaa caacctatgc aattcagttc ttagctttta aatgtggcat gcttccatgc
     7081 caaggcccac actgaaatgc tcaaatggga gacaaagaga ttaagctctt atgtaaaatt
     7141 tgctgtttta cataacttta atgaatggac aaagtcttgt gcatgggggt gggggtgggg
     7201 ttagagggga acagctccag atggcaaaca tacgcaaggg atttagtcaa acaacttttt
     7261 ggcaaagatg gtatgatttt gtaatggggt aggaaccaat gaaatgcgag gtaagtatgg
     7321 ttaatgatct acagttattg gttaaagaag tatattagag cgagtctttc tgcacacaga
     7381 tcacctttcc tatcaacccc actagcctct ggcaaaatga agtgggtaac ctttctcctc
     7441 ctcctcttcg tctccggctc tgctttttcc aggggtgtgt ttcgccgaga agcacgtaag
     7501 agttttatgt tttttcatct ctgcttgtat ttttctagta atggaagcct ggtattttaa
     7561 aatagttaaa ttttccttta gtgctgattt ctagattatt attactgttg ttgttgttat
     7621 tattgtcatt atttgcatct gagaaccctt aggtggttat attattgata tatttttggt
     7681 atctttgatg acaataatgg gggattttga aagcttagct ttaaatttct tttaattaaa
     7741 aaaaaatgct aggcagaatg actcaaatta cgttggatac agttgaattt attacggtct
     7801 catagggcct gcctgctcga ccatgctata ctaaaaatta aaagtgtgtg ttactaattt
     7861 tataaatgga gtttccattt atatttacct ttatttctta tttaccattg tcttagtaga
     7921 tatttacaaa catgacagaa acactaaatc ttgagtttga atgcacagat ataaacactt
     7981 aacgggtttt aaaaataata atgttggtga aaaaatataa ctttgagtgt agcagagagg
     8041 aaccattgcc accttcagat tttcctgtaa cgatcgggaa ctggcatctt cagggagtag
     8101 cttaggtcag tgaagagaag aacaaaaagc agcatattac agttagttgt cttcatcaat
     8161 ctttaaatat gttgtgtggt ttttctctcc ctgtttccac agacaagagt gagatcgccc
     8221 atcggtataa tgatttggga gaacaacatt tcaaaggcct gtaagttata atgctgaaag
     8281 cccacttaat atttctggta gtattagtta aagttttaaa acaccttttt ccaccttgag
     8341 tgtgagaatt gtagagcagt gctgtccagt agaaatgtgt gcattgacag aaagactgtg
     8401 gatctgtgct gagcaatgtg gcagccagag atcacaaggc tatcaagcac tttgcacatg
     8461 gcaagtgtaa ctgagaagca cacattcaaa taatagttaa ttttaattga atgtatctag
     8521 ccatgtgtgg ctagtagctc ctttcctgga gagagaatct ggagcccaca tctaacttgt
     8581 taagtctgga atcttatttt ttatttctgg aaaggtctat gaactatagt tttgggggca
     8641 gctcacttac taacttttaa tgcaataaga tctcatggta tcttgagaac attattttgt
     8701 ctctttgtag tactgaaacc ttatacatgt gaagtaaggg gtctatactt aagtcacatc
     8761 tccaacctta gtaatgtttt aatgtagtaa aaaaatgagt aattaattta tttttagaag
     8821 gtcaatagta tcatgtattc caaataacag aggtatatgg ttagaaaaga aacaattcaa
     8881 aggacttata taatatctag ccttgacaat gaataaattt agagagtagt ttgcctgttt
     8941 gcctcatgtt cataaatcta ttgacacata tgtgcatctg cacttcagca tggtagaagt
     9001 ccatattcct ttgcttggaa aggcaggtgt tcccattacg cctcagagaa tagctgacgg
     9061 gaagaggctt tctagatagt tgtatgaaag atatacaaaa tctcgcaggt atacacaggc
     9121 atgatttgct ggttgggaga gccacttgcc tcatactgag gtttttgtgt ctgcttttca
     9181 gagtcctgat tgccttttcc cagtatctcc agaaatgctc atacgatgag catgccaaat
     9241 tagtgcagga agtaacagac tttgcaaaga cgtgtgttgc cgatgagtct gccgccaact
     9301 gtgacaaatc ccttgtgagt accttctgat tttgtggatc tactttcctg ctttctggaa
     9361 ctctgtttca aagccaatca tgactccatc acttaaggcc ccgggaacac tgtggcagag
     9421 ggcagcagag agattgataa agccagggtg atgggaattt tctgtgggac tccatttcat
     9481 agtaattgca gaagctacaa tacactcaaa aagtctcacc acatgactgc ccaaatggga
     9541 gcttgacagt gacagtgaca gtagatatgc caaagtggat gagggaaaga ccacaagagc
     9601 taaaccctgt aaaaagaact gtaggcaact aaggaatgca gagagaagaa gttgccttgg
     9661 aagagcatac caactgcctc tccaatacca atggtcatcc ctaaaacata cgtatgaata
     9721 acatgcagac taagcaggct acatttagga atatacatgt atttacataa atgtatatgc
     9781 atgtaacaac aatgaatgaa aactgaggtc atggatctga aagagagcaa gggggcttac
     9841 atgagagggt ttggagggag gggttggagg gagggaggta ttattcttta gttttacagg
     9901 gaacgtagta aaaacatagg cttctcccaa aggagcagag cccatgagga gctgtgcaag
     9961 gttccccagc ttgattttac ctgctcctca aattccctta aggcgcataa cgataccacg
    10021 atatcaacaa gtttgtacaa aaaagcaggc tggcgccgga accgaagttc ctattccgaa
    10081 gttcctattc tctagaaagt ataggaactt cgaacccttt cccacaccac cctccacact
    10141 tgccccaaac actgccaact atgtaggagg aaggggttgg gactaacaga agaacccgtt
    10201 gtggggaagc tgttgggagg gtcactttat gttcttgccc aaggtcagtt gggtggcctg
    10261 cttctgatga ggtggtccca aggtctgggg tagaaggtga gagggacagg ccaccaaggt
    10321 cagccccccc cccctatccc ataggagcca ggtccctctc ctggacagga agactgaagg
    10381 ggagatgcca gagactcagt gaagcctggg gtaccctatt ggagtccttc aaggaaacaa
    10441 acttggcctc accaggcctc agccttggct cctcctggga actctactgc ccttgggatc
    10501 cccttgtagt tgtgggttac ataggaaggg ggacgggatt ccccttgact ggctagccta
    10561 ctcttttctt cagtcttctc catctcctct cacctgtctc tcgacccttt ccctaggata
    10621 gacttggaaa aagataaggg gagaaaacaa atgcaaacga ggccagaaag attttggctg
    10681 ggcattcctt ccgctagctt ttattgggat cccctagttt gtgataggcc ttttagctac
    10741 atctgccaat ccatctcatt ttcacacaca cacacaccac tttccttctg gtcagtgggc
    10801 acatgtccag cctcaagttt atatcaccac ccccaatgcc caacacttgt atggccttgg
    10861 gcgggtcatc ccccccccca cccccagtat ctgcaacctc aagctagctt gggtgcgttg
    10921 gttgtggata agtagctaga ctccagcaac cagtaacctc tgccctttct cctccatgac
    10981 aaccaggctc ccaggtcccg aaaaccaaag aagaagaacg cagatctcta gagtcaccga
    11041 gttgctttac cggatgaaga gggccgaaac atactgtcca aggcccttgc tggcaatcca
    11101 cccaactgaa gccagacaca aacagaaaat tgtggcaccg gtgaaacaga ctttgaattt
    11161 tgaccttctc aagttggcgg gagacgtcga gtccaacccc ggaccggtgc atgtggaccc
    11221 caagaaaaag cggaaggtgg accctaaaaa gaaacgcaaa gtggatccta agaaaaaaag
    11281 aaaggtgcac gtgtccatgg tgtccaaggg cgaggaactg ttcaccggcg tggtgcccat
    11341 cctggtggaa ctggatggcg acgtgaacgg ccacaagttc agcgtgtccg gcgagggcga
    11401 aggcgacgcc acatacggaa agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
    11461 cgtgccttgg cctaccctcg tgaccacact gacctacggc gtgcagtgct tcagcagata
    11521 ccccgaccat atgaagcagc acgacttctt caagagcgcc atgcccgagg gctacgtgca
    11581 ggaaagaacc atcttcttta aggacgacgg caactacaag accagggccg aagtgaagtt
    11641 cgagggcgac accctcgtga acagaatcga gctgaagggc atcgacttca aagaggacgg
    11701 caacatcctg ggccacaagc tggagtacaa ctacaacagc cacaacgtgt acatcatggc
    11761 cgacaagcag aaaaacggca tcaaagtgaa cttcaagatc cggcacaaca tcgaggacgg
    11821 ctccgtgcag ctggccgacc actaccagca gaacacccct atcggcgacg gccctgtgct
    11881 gctgcctgac aaccactacc tgagcaccca gagcgccctg agcaaggacc ccaacgagaa
    11941 gagggaccac atggtgctgc tggaattcgt gaccgccgct ggcatcaccc tgggcatgga
    12001 cgagctgtac aagcctgcag gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga
    12061 ggagaatccc gggccgtcca atttactgac cgtacaccaa aatttgcctg cattaccggt
    12121 cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac atgttcaggg atcgccaggc
    12181 gttttctgag catacctgga aaatgcttct gtccgtttgc cggtcgtggg cggcatggtg
    12241 caagttgaat aaccggaaat ggtttcccgc agaacctgaa gatgttcgcg attatcttct
    12301 atatcttcag gcgcgcggtc tggcagtaaa aactatccag caacatttgg gccagctaaa
    12361 catgcttcat cgtcggtctg ggctgccacg accaagtgac agcaatgctg tttcactggt
    12421 tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa cgtgcaaaac aggctctagc
    12481 gttcgaacgc actgatttcg accaggttcg ttcactcatg gaaaatagcg accgctgcca
    12541 ggatataagg aatctggcat ttctggggat tgcttataac accctgttac gtatagccga
    12601 aattgccagg atcagggtta aagatatctc acgtactgac ggtgggagaa tgttaatcca
    12661 tattggcaga acgaaaacgc tggttagcac cgcaggtgta gagaaggcac ttagcctggg
    12721 ggtaactaaa ctggtcgagc gatggatttc cgtctctggt gtagctgatg atccgaataa
    12781 ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg ccatctgcca ccagccagct
    12841 atcaactcgc gccctggaag ggatttttga agcaactcat cgattgattt acggcgctaa
    12901 ggatgactct ggtcagagat acctggcctg gtctggacac agtgcccgtg tcggagccgc
    12961 gcgagatatg gcccgcgctg gagtttcaat accggagatc atgcaagctg gtggctggac
    13021 caatgtaaat attgtcatga actatatccg taacctggat agtgaaacag gggcaatggt
    13081 gcgcctgctg gaagatggcg atctcgagcc atctgctgga gacatgagag ctgccaacct
    13141 ttggccaagc ccgctcatga tcaaacgctc taagaagaac agcctggcct tgtccctgac
    13201 ggccgaccag atggtcagtg ccttgttgga tgctgagccc cccatactct attccgagta
    13261 tgatcctacc agacccttca gtgaagcttc gatgatgggc ttactgacca acctggcaga
    13321 cagggagctg gttcacatga tcaactgggc gaagagggtg ccaggctttg tggatttgac
    13381 cctccatgat caggtccacc ttctagaatg tgcctggcta gagatcctga tgattggtct
    13441 cgtctggcgc tccatggagc acccagtgaa gctactgttt gctcctaact tgctcttgga
    13501 caggaaccag ggaaaatgtg tagagggcat ggtggagatc ttcgacatgc tgctggctac
    13561 atcatctcgg ttccgcatga tgaatctgca gggagaggag tttgtgtgcc tcaaatctat
    13621 tattttgctt aattctggag tgtacacatt tctgtccagc accctgaagt ctctggaaga
    13681 gaaggaccat atccaccgag tcctggacaa gatcacagac actttgatcc acctgatggc
    13741 caaggcaggc ctgaccctgc agcagcagca ccagcggctg gcccagctcc tcctcatcct
    13801 ctcccacatc aggcacatga gtaacaaagg catggagcat ctgtacagca tgaagtgcaa
    13861 gaacgtggtg cccctctatg acctgctgct ggaggcggcg gacgcccacc gcctacatgc
    13921 gcccactagc cgtggagggg catccgtgga ggagacggac caaagccact tggccactgc
    13981 gggctctact tcatcgcatt ccttgcaaaa gtattacatc acgggggagg cagagggttt
    14041 ccctgccaca gcttgatgaa gatctgagct cttaattaat gatcataatc agccatatca
    14101 catctgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
    14161 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
    14221 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    14281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgtttaaac taactttaaa
    14341 taattggcat tatttaaagt tacatatggc ggccaattcg ccttctgcag gagcgtacag
    14401 aacccagggc cctggcaccc gtgcagaccc tggcccaccc cacctgggcg ctcagtgccc
    14461 aagagatgtc cacacctagg atgtcccgcg gtgggtgggg ggcccgagag acgggcaggc
    14521 cgggggcagg cctggccatg cggggccgaa ccgggcactg cccagcgtgg gcgcgggggc
    14581 cacggcgcgc gcccccagcc cccgggccca gcaccccaag gcggccaacg ccaaaactct
    14641 ccctcctcct cttcctcaat ctcgctctcg ctcttttttt ttttcgcaaa aggaggggag
    14701 agggggtaaa aaaatgctgc actgtgcggc gaagccggtg agtgagcggc gcggggccaa
    14761 tcagcgtgcg ccgttccgaa agttgccttt tatggctcga gcggccgcgg cggcgcccta
    14821 taaaacccag cggcgcgacg cgccaccacc gccgagaccg cgtccgcccc gcgagcacag
    14881 agcctcgcct ttgccgatcc tctagagtcg agatccgccg ccaccatgat tgaacaagat
    14941 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
    15001 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
    15061 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
    15121 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
    15181 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
    15241 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
    15301 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
    15361 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
    15421 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
    15481 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
    15541 ttcatcgact gtggccggct gggtgtggcc gaccgctatc aggacatagc gttggctacc
    15601 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
    15661 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
    15721 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
    15781 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
    15841 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccccggatc
    15901 taagctctag ataagtaatg atcataatca gccatatcac atctgtagag gttttacttg
    15961 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
    16021 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    16081 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    16141 tatcttatca tgtctggatc cctaacttta aataatgcca attatttaaa gttaggaatt
    16201 aattcaccca gctttcttgt acaaagtggt tgatatctct atagtcgcag taggcggagg
    16261 ccattagctc tttgcttctc tgtgttatcc atcatacaca atattgtggc acttgactat
    16321 catgaaaatt acacagaaat tgacaagtcc aaggaaagta agtggtaggg ggtttctaca
    16381 tgatcttcgt ttgcctcgca gtttaaaaac gtaacttact tcattaacaa tttttttata
    16441 cttaaattta ttttaagtgg tatgtagaac caaaaaaata atatatagct caaggtgtta
    16501 tcaatataca tatcatctat gcacatacat acaaggtaca tgttttcttc accaattttt
    16561 atcagcacat ttggttttga tgctgctctt tggctatgaa gaaagttggg gtaccggcag
    16621 gaatttgact tttctctctt cctcttagca cactcttttt ggagataagt tgtgtgccat
    16681 tccaaacctc cgtgaaaact atggtgaact ggctgactgc tgtacaaaac aagagcccga
    16741 aagaaacgaa tgtttcctgc aacacaaaga tgacaacccc agcctgccac catttgaaag
    16801 gccagaggct gaggccatgt gcacctcctt taaggaaaac ccaaccacct ttatgggaca
    16861 gtgagtaccc agacatctac actttatgct aaaaactggg caatagctcc gttagtaaca
    16921 cttatgctga tagtcatgaa aaccccaagc atccaacacc aagtctggga atgtgttgag
    16981 atggtgttta aaaacataat acttgatacc acagactata caatgacagt aaaaagatca
    17041 atgatgtttt gatattgaaa acaggacctg tgtgaaatat ctgagactga gaagttgcaa
    17101 aatattctga aggcatttgt gtgcatgtgc gtgtatatgt gtgtgagtat gagtatgtgt
    17161 gtggtgttca tgttctctgg atcaatttca ttttagtcat caacattaca tcaccaaaat
    17221 ttacttacca aaaatgtaca catgatgtgt gaacatttac aaacacacac gcgcacacac
    17281 acacacacac acacacacac acacacacac acacacacac acacatatct acatatctct
    17341 ctttgctcac ttaacaacag acaaagtcca tcactcatgg gtgaccacac tgagtgaaac
    17401 cggtcaatta caaatcccat gtgtatccaa gggtgtgggt tgattctaat catttctgta
    17461 gtattgttct gtggggaata actgcctgtt ttcttcttct tctttcccat aactcagcta
    17521 tttgcatgaa gttgccagaa gacatcctta tttctatgcc ccagaacttc tttactatgc
    17581 tgagcagtac aatgagattc tgacccagtg ttgtgcagag gctgacaagg aaagctgcct
    17641 gaccccgaag gtaaactcct ggaaagacac acacaacacc tgctttgaat tccagcctaa
    17701 gattttgatc atcttatggc cagattcagg aggcttggct ttctgcatga aacatctctt
    17761 tcttcttaac ttaccaacag atttttgttc ttatgccttt cttccttcta aactttagca
    17821 gtggtctgag agagggaatt cttgtacttg cgttccttgt cttctcattc ttcatggtaa
    17881 tgctgtctac agtagaagac tctttgtcaa actgtgcaga aatggctagg ttttttttct
    17941 ttgtttttgc ttttattttt tgaccttctc tgaggcattt tactggagct ttcttaaatt
    18001 cgaaggagag aatttttact gaggctagaa gttcgagttc attccttcct tccataaata
    18061 tttgttacat gcagcattca ttttttggag gcacttactg attttatgaa agttaatgga
    18121 ggaaggactg aaatggatta gtcacaaaat aaaaaaagtc agtacaggta tatgtgctgg
    18181 gacatctcct ttccggaccc ttccctcagc aatgtctcca aggtgcttta agtagctaga
    18241 tgctaagacc ggacagattt tcttttccct tagatatcaa aataattatt tgccagaagg
    18301 gagtgcttgg gaatggaggc ataaattatc ttttttaagt tttcatttgg atcgaaggtc
    18361 caactaaatg gcagagttca tacctaacat gcaaaaggtc ccgatttcaa ctcctcccca
    18421 accaaaccaa ttatttaggc atagggccaa ctgaaacttt gggaggtgtg tgtgtataaa
    18481 aataatcttt tccaattttt ttccacttgg gttttctttt ttgtttgtct gtttttagct
    18541 tgatggtgtg aaggagaaag cattggtctc atctgtccgt cagagaatga agtgctccag
    18601 tatgcagaag tttggagaga gagcttttaa agcatggtac atgacttttc tttaacatca
    18661 cagttcgcat ctttctgttg atagctgtct aatcaaaaaa gctgtgcctt tataaggcac
    18721 tttatgaaaa gacaatagag gtgaagtcag aatagcagta ataatttgac atttttgtct
    18781 catcgttttg ttttagagat ggcgcaacgc aattaatgat aacttcgtat agcatacatt
    18841 atacgaagtt atggtctgag ctcgccatca gttcagggtg atgaatttac ttaatttatg
    18901 ttccctcagt ctaaaactga ctgttttatg tcttgataat tgaatttagc catcaagcca
    18961 gctggagtta ctacttcgaa ttttattaca actccaattg tgttcttaat ctcatgcttt
    19021 tgtttgaccc tctctttgct tttatagctg tagctgagtc tgggtgcacc ctttaatatg
    19081 gcagccaaga aagatatgct tgctcttctg gtacctcacc taaccaacac aataggagag
    19141 aaggttgaga tgactttgtg tgtgttctct agaaactgtc tgcacaactt tcctcactaa
    19201 gtcattccca ctctccaaat gattacagcc agatcaacca aagaagcagc ttcaatatga
    19261 aactgttaca cgggctgtga ctggctcctg caaaggagtc ttttactgga gtaaaggaac
    19321 tgaaagttat tttagaatga gaatcttggg ttttgccata gggctcagag gctgtctgct
    19381 ggaacagctt ctaggcagga ggatgcgtgt ttagaactct ccagttctct agaacttaag
    19441 aacacccaca accccccaac tttaggactc agagaaatat gtcttatggt aaaagatgac
    19501 gaatcttcat ttccagatga gaatgctttc atgccattac gtaatttaat ctcccagatt
    19561 ctgcatgttg taacactcta aaagctaacg cctgctggtg gtcacagtgc taagatggga
    19621 tcaaactgaa aacagaagtc agataaggac ttaaagcttt actgctgccc ctttggggaa
    19681 aatcttctat gattttaatt taatgaggtc ttagctttcc acacaccacc gccattcatt
    19741 tagtagataa ttctgccaaa gaaaaacata actatcatgc cagattagca ttacaagaac
    19801 taatgaattt ttataatttg ttcagttaat ttggttttga acttcaaaac agtttataat
    19861 aggttttgaa cttgttaaaa atagtgtctt ttgttattgc tttcatacaa atacaatatg
    19921 ataattcatt gatggaaaat ctgtttgttt gtttttcctt ttcagggcag tagctcgtct
    19981 gagccagaca ttccccaatg ctgactttgc agaaatcacc aaattggcaa cagacctgac
    20041 caaagtcaac aaggagtgct gccatggtga cctgctggaa tgcgcagatg acagggtaag
    20101 gaaggagtca ttacatgctc agttgagcgc ctgtgtttgg cttcatcgga gaacaccaag
    20161 ggcgggtggt gtggccggta ttctaccaag ctctccttgg ttgctgccac tttccagaaa
    20221 aaaagaccaa aacaaacaaa caaacaaaca aacaaacaaa caaaaaactc cccccaaaaa
    20281 tccaaaaaac aaaaccacca actcaaaatg ttttcggaat tattcaatga cccgaaactc
    20341 ctttcttcag tcacactttg agaaggcatt aatggaagag aacaagccat aaaatgtaga
    20401 tttctaagag gttttgggga gagaagcagg ggctagacat cccacagagc agagaggctt
    20461 ttaaaaatgt gtctatccct tggtggtctt tcccctaatc tactgtctgc gttaagtaca
    20521 tacagattca ctgtgatctc attttctcca acattgtcct attatctccc tcccctccct
    20581 gctccaaaga ctaaaaatat aaccaaatac cagaatcctc ttctaccata gtaccttccg
    20641 gaaaactcaa gacagatatt ttgtatatgt cagcaagacc tgtaattgtg ctcattactg
    20701 attatttaat aatacttcat attctaatgg gttatttagt ttcaaaatgc ctacccttaa
    20761 cttgggagaa aatttttttt caagcactaa cttttataga aaaagtaact tgaattatca
    20821 aagtcagaaa gacatagatt taataattaa aacaaactaa tttaaataga tacatagctt
    20881 gcttcagaac ggtctttctc ggatctaaat ggaatgtttt gatcatagta tcctagtcca
    20941 caggttctgc agcactgcag ggctgagaac gagtttacag aaaccacttt ggttgtgtct
    21001 ggtttagggc aggatgaaga cactttcctc acatgacaga ggtgcaggga caaaatgtag
    21061 ctaagataat tcctctgaaa cctttgtttt tcattctcac aggcggaact tgccaagtac
    21121 atgtgtgaaa accaggcgac tatctccagc aaactgcaga cttgctgcga taaaccactg
    21181 ttgaagaaag cccactgtct tagtgaggtg gagcatgaca ccatgcctgc tgatctgcct
    21241 gccattgctg ctgattttgt tgaggaccag gaagtgtgca agaactatgc tgaggccaag
    21301 gatgtcttcc tgggcacgtg agtagatgcc tttcctttca tagctttgtc agagctgcat
    21361 ggtttaggac tctacaccag gctttccttt ggtgttagca ggacttctat ggttctcaga
    21421 aaaattattc atccttgcca ttttcagcct ttaaaatttc cttgaaaagt attttaagta
    21481 atttaaaata tttttttatg aaacatacat gcattcagca taaactcaat gttccttact
    21541 tcataggaat atttctggaa atctttagaa atattctatg aatatgtaag tcatatgtac
    21601 cacttaatca tttttatgag catatatctt aaatacatag tgtatataaa tatatcacct
    21661 atctatctac ttacatactc agctctatct agctagctct gtgatgatat caaggatatc
    21721 tatttctatg tatataagca acatgacact agttgtttca tccttggttg aaaagaccag
    21781 aatatcacaa tatagcaaga catgatgcaa ctaataacaa atgacaaaat attttcaaaa
    21841 agtacagcaa ttatttccat tgtacaatgg agggagttaa tgcattgatt gattgattga
    21901 ttgattgatt gatagttgcc cacccagaat attaaaacag gtttcagtgg caaagctgaa
    21961 cgtgggaaga gttctctctg ggtcccacat tcttgttgtt tagttttctg catggagctc
    22021 gctccaatgc aggatgactg ggctgagaat cactactgag gttggttatg gggctctatt
    22081 acatattcat ttacttaact cagcctatgt tcatctcaat taggttcttg tatgaatatt
    22141 caagaagaca ccctgattac tctgtatccc tgttgctgag acttgctaag aaatatgaag
    22201 ccactctgga aaagtgctgc gctgaagcca atcctcccgc atgctacggc acagtggtag
    22261 gtttccatga gccaagaaca cctacagtgt ggatggcact ttctataagg acgagggaag
    22321 agatgaagtc acgataatcg atatgtaggt caatacagat gttttcttga gatggaggca
    22381 agttctagct ctttaagttc ttcagcatca taggataaac tctttggata aaatataagg
    22441 aaacctagaa caaccgctct agaaaagaaa ggttcaaaga gagggaggaa tcttttcttg
    22501 cacaaaatct ttattgtagc tttccctttt tcatactccc aaggataaaa ttagagaata
    22561 aagtccaatg ccaactaaaa t
//