LOCUS       84554#pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21564 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 84554
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000039631
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8092..8202
                     /db_xref="ENSEMBL:ENSMUSE00000545192"
                     /rank="4"
                     /note="ENSMUSE00000545192"
     exon            4768..4896
                     /db_xref="ENSEMBL:ENSMUSE00000545193"
                     /rank="3"
                     /note="ENSMUSE00000545193"
     misc_feature    4275..9425
                     /note="5 arm"
     misc_feature    9453..9477
                     /note="B1 Gateway"
     misc_feature    9490..9537
                     /note="Frt"
     misc_feature    9538..10037
                     /note="mouse En2 intron"
     primer          complement(9606..9627)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9502..9525)
                     /note="FRT3"
     primer          13689..13707
                     /note="seq primer NF"
     misc_feature    13749..13756
                     /note="RE sites"
     misc_feature    15549..15582
                     /note="loxP"
     misc_feature    complement(13757..13788)
                     /note="Rox"
     misc_feature    complement(15509..15540)
                     /note="Rox"
     misc_feature    13789..13810
                     /note="polylinker"
     CDS             14369..14965
                     /note="puromycin N-acetyl transferase"
     misc_feature    14484..14489
                     /note="Mutated RsrII site"
     intron          10038..10412
                     /note="mouse En2 intron"
     misc_feature    10410..10412
                     /note="Splice Acceptor"
     exon            10413..10452
                     /note="mouse En2 exon"
     primer          10313..10334
                     /note="seq primer FCHK2"
     misc_feature    10453..10575
                     /note="1Dleader"
     misc_feature    10576..10627
                     /note="F2A"
     misc_feature    10723..11439
                     /note="EGFP"
     misc_feature    10633..10722
                     /note="3X NLS"
     misc_feature    13505..13743
                     /note="SV40 polyadenylation site"
     misc_feature    12538..13482
                     /note="ERT2"
     promoter        13821..14332
                     /note="PGK promoter"
     misc_feature    15076..15508
                     /note="PGK pA"
     primer          13563..13586
                     /note="LRPCR RAF5"
     misc_feature    11449..11502
                     /note="T2A"
     misc_feature    11499..11499
                     /note=" A to G to kill RsRII site"
     misc_feature    11503..12528
                     /note="Cre"
     misc_feature    10428..10453
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    10428..10443
                     /note="FCHK QC prefix region"
     misc_feature    10444..10447
                     /note="FCHK QC critical region"
     misc_feature    10448..10453
                     /note="FCHK QC suffix region"
     misc_feature    15605..15625
                     /note="B2 Gateway"
     misc_feature    9426..15655
                     /note="Synthetic Cassette"
     exon            17707..17898
                     /db_xref="ENSEMBL:ENSMUSE00000545189"
                     /type="targeted"
                     /rank="7"
                     /note="target exon 1 ENSMUSE00000545189"
     exon            16571..16732
                     /db_xref="ENSEMBL:ENSMUSE00000545190"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 2 ENSMUSE00000545190"
     exon            16074..16158
                     /db_xref="ENSEMBL:ENSMUSE00000545191"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 3 ENSMUSE00000545191"
     misc_feature    15656..18049
                     /note="Critical Region"
     misc_feature    18073..18106
                     /note="Downstream LoxP"
     misc_feature    18050..18129
                     /note="synthetic loxP region"
     exon            20976..21564
                     /db_xref="ENSEMBL:ENSMUSE00000266964"
                     /rank="9"
                     /note="ENSMUSE00000266964 5' fragment"
     exon            19288..19469
                     /db_xref="ENSEMBL:ENSMUSE00000545188"
                     /rank="8"
                     /note="ENSMUSE00000545188"
     misc_feature    18130..21564
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggacacaac tgtgtattgc tttctaccca gagtttacaa aacctcatga
     4321 aaaacatatc tgtgatagtc tgggatataa cctagtggtt caaggtgcac acacacacac
     4381 acacgcacac acacacacac acacacacat acacatcaca tcacacacat cacactggtg
     4441 ataattaata ctgaggaata aatgccaatt ttaagtatca ggcagaaaga ttacatgatc
     4501 acagccactg tggtcatttt tctcactaga gtggcagcag cagctgtggt tgttataaac
     4561 tgtccctatg tacaccatta ccactttggg ggaagaaact aagacaaaag aaagccctgg
     4621 ctggctactc accctcatag agtttgatct tagatgaaag gaatcactct tattaaatgt
     4681 aactttatat tgtgtcagct gttacagaag tcatcaattc acacgtttcg ggaaagcact
     4741 gacttgtggt atgttgaatc attctaggga gtaatggttg gaatgggcca aaaagacagc
     4801 tatgtggggg atgaagccca gagcaagaga gggatcctga cgctgaagta tccgatagaa
     4861 cacggcatca tcaccaactg ggacgacatg gaaaaggtac caccccaggg aatcagagct
     4921 gtgcactgca gcctggcaag gctgcagaac acagctagct atccttgctc aagtgccttg
     4981 ctattgctag ttgttaatat ttaacttgga tggctacggt aaaattggta tttataaact
     5041 gcttgttggg agacagttct atagttgaga gagccctttg ttactgaagg atagagtctc
     5101 tcttcaagcc cactggactg acctctgtca gcacaagctg tccagaggta ggaatctggg
     5161 aagacctagc cagcccattc ttgagagttg tttattatta ttattattat ttatttccca
     5221 tgagcagttt tcttaatgtt cacaactgaa aagagatcgc tatgtatttg atacattccc
     5281 aaatgcctgt gaacatattc aagtgcaatt gtgactaagt ctttaaacga atactcttaa
     5341 attctctttc tcttccacaa taagaccctt cttcccagac cattgtggaa gagtcagtgg
     5401 gggaaaaaaa gagtcaaatc aaaggattga ttatgaagct tgtttttagg cattttagaa
     5461 acactagaga taggaaaaga aagcaaagaa ccaattagaa actgacatgt ttggtttttt
     5521 ttttgttgtt gttgtttttt gttttttttt aactgctgtg gaacataact gcaacatctg
     5581 gtttttattt atctgcttat taggtttttg tatcctcccc aaaacataca ctcatccaca
     5641 caagctccca cgtatcgttt aaaaataaaa ccaacacata tcactactat agtgtattgg
     5701 taattaagtt taaacctttc tccctctgca agggctgccc atccttgcct ttagttagtc
     5761 tgactgtagg tttggtgcag ctctagttcg aatcctggag atggcagcaa catagggtgg
     5821 agcgattgtg gggggagggt ctctctgtgg agcccatgac atctctgcat ggatggtcca
     5881 aggtggggga tagagagaga gagagagagt gagagtgaga gagagagaga gagagagaga
     5941 gagagagaga gagaatgctc ttatattcta gcttttaaat tcaaagcaaa ctctgcagaa
     6001 aagcaatgaa ggagggagtg gggcttttga agcttgtttg aggtagccag agtgtagagg
     6061 agatggctga ctagggctag tgagcactct attttttcct tgttttatag tgtttttttt
     6121 ttaatttttt attagatatt ttcttcattt acatttcaaa tactatccca aaagtcacct
     6181 ataccctccc gccgccctgc tcccctaccc actcactccc actttttggc cctgtcattc
     6241 ccctctactg gggcatataa agtttgcaag accaaggggc ctctcttccc aatgaaagct
     6301 gactaggcca tcttctgcta catatgcagc tagagacacg agttctgggg gtactggtta
     6361 gttcatattg ttgttccacc tatagggttg cagacccctt cagctccttg ggtactttct
     6421 ctagctcctt cattgggggc cctgtgtttt atcctataga tgactgtgag catccacttc
     6481 tgtatttgcc aagcactggc atagccccac aagggagagc tatgtcaggg ttcttccagc
     6541 aaaatcttgc tggcatatgc actagtaagg atatcatcct gagtaaggta acccaatcac
     6601 aaaagaagtc acttgatata cactcactga taagtggata ttggcccaga aacctagaat
     6661 acccaagata caatttgcaa aacacaagaa aatcaaggag aaggaagacc aacatgtgga
     6721 tacttcattc cttcctagaa tagggaataa aatacccacg gaaggagtta cagagacaaa
     6781 gtttggagct aagacgaaag aatggaccat ccagagactg ccccacccgg gggtccatcc
     6841 aataatcagc caccaatcac agacacttga gtagggtgtt tatcctgctg atttatctct
     6901 tttggtggaa caagatgaag agataaagga tgagttcccc actcttcttt actgtactga
     6961 ggaattatga acatggtttt ccaactgcca gccctgccct ttcttcatct tacccacccc
     7021 cgcaaatgac aggatttgta ggtgccccct tagtttgctg gaaaccacaa aaccaaaacc
     7081 cagaaactag aaacttgaca aggcctttgc agttggtttt acttttatta gtttataccc
     7141 tgtcattagt tatgcttgtt tacttctggc caatctcata ttcctgatgc tggtggacaa
     7201 tggcccttcc ggcccttcca tagcaggtga attatttcag ccccttccat aacaatgggt
     7261 tctttttgac actaaggggt taaagaggtt gcacaactat aaagccaaca cacatttgtc
     7321 caaaatgcat tcatacagta cacacaggtg ggcatgggct tttgggggaa ccttctcccc
     7381 acctgcatat acccatctac cacacacaca cacacacaca cacacacaca cacacacaca
     7441 cacaccaaca caaacagcca agaacactct ctagaagcat taaagatggc aagtaggttt
     7501 tcaggcaagc ctcattcatt tatatgtatg gtcatttgtc cttttatgta ttacactaaa
     7561 tcaacttccc atgaataaac taaggtgaag gcatgtatga agaaggcgaa actaatgaat
     7621 gaaagcatct attctagccc agacactggg ctaaggactt attctagtat cttttttttg
     7681 taattcacac aaagagcaaa tgacatctat actggttatt ctcttctgtt accaacgggg
     7741 agactagaga tggaaagaac tatgaatttg ctagctcaca acaggaagtt gtgacagaaa
     7801 cttgaaagtt cctgctgagg agcaggaagt tgactacaga gcctatgtcc ttgacaaata
     7861 cgccattctg ctacaccaat agttgagcaa tattgagttg ctgagtcact caatagatgt
     7921 acatacagtc tcgtatatgg tgttcactct ctgggcttct gcatctgtac agatgaggtc
     7981 ttgtgcattt tatccggttc tagttttgtc ctgaacatcc ctgtcccttt ccaggacctt
     8041 ttgcatccca caagaacaga ttaccacgct cctctttacc catctctcta gatctggcac
     8101 cactctttct ataacgagct tcgtgtggcc cctgaagagc atccgacact gctgacagag
     8161 gcaccactga accctaaggc caaccgggag aaaatgaccc aggtaaatat ttgctccagg
     8221 atctaaccct ggtgaatttg gggcatgggc aaccaggata acttgcagca cgacaggcag
     8281 acataaggtc caaactgaca gttcaccaac agactgaccc aggaacccca aagccccgga
     8341 atcttagtgt tcacaccctt actccacaaa acaacataat tttgcctcaa agggcaagaa
     8401 gaaaagctta aaggtttaag attagggcta aaaacttctc ataagtcatt gaaaatgcag
     8461 gatgatatag tagggattga atctgttact tatcagttac caaaagccac ttcttttctt
     8521 aggacccctg cttcttttct tttttccttt tttttttttt tttttttttt ttttttttgg
     8581 ttaggaatat caacaccatt atttttcaag ggatagatat gtaggctaag tgatgccaaa
     8641 attatatgca agtatcatgg ggtgtggtat gataaatatc caacaaggag taacaaagac
     8701 tgtcactact gtctgtgtga attctaagac catacaccta gggaacgtcc agtgaaccag
     8761 ctagagagtt ctgatgtgga tgagccgata aatgatagca tctactgtca gttcctcaca
     8821 ggccctggtc aagcacaggg tgttgcaagt gcactctaac tcctaatgat ttaaggcagg
     8881 aactattatc ctaactacct cagtgatgaa attaaggctc agatgcacaa agtgattttc
     8941 ctaaagtcac atagctaggc gtggcaatgc ctagagttga cccatgaatt ttccccttga
     9001 agaatagcaa atgcacacag cccagggccc cctcctggga agagcatttg ctgtgctcac
     9061 cagcacatca gctgttctgg gtggtgagga gcatcctgta aattgcatga aggtggaaaa
     9121 atgactgaga actatggagt tttagcaaat atcagacatt ccttctggtt tgtaaactag
     9181 agagttattt aaatgcagac tgcggacatg ttctcatgta ggagggatct agggagctcc
     9241 agtttttctc ttgtccctga attgcatggg ctattgggtc gacatttata tgtagtcctc
     9301 tattccatag tataggtcgt atctgccagg atcatagcct cacccactac agctgcaggg
     9361 tggagtctgg ttccatggat agcattgacc ccaaattgca taaaaccatg gagaagatca
     9421 aactgaaggc gcataacgat accacgatat caacaagttt gtacaaaaaa gcaggctggc
     9481 gccggaaccg aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcgaa
     9541 ccctttccca caccaccctc cacacttgcc ccaaacactg ccaactatgt aggaggaagg
     9601 ggttgggact aacagaagaa cccgttgtgg ggaagctgtt gggagggtca ctttatgttc
     9661 ttgcccaagg tcagttgggt ggcctgcttc tgatgaggtg gtcccaaggt ctggggtaga
     9721 aggtgagagg gacaggccac caaggtcagc cccccccccc tatcccatag gagccaggtc
     9781 cctctcctgg acaggaagac tgaaggggag atgccagaga ctcagtgaag cctggggtac
     9841 cctattggag tccttcaagg aaacaaactt ggcctcacca ggcctcagcc ttggctcctc
     9901 ctgggaactc tactgccctt gggatcccct tgtagttgtg ggttacatag gaagggggac
     9961 gggattcccc ttgactggct agcctactct tttcttcagt cttctccatc tcctctcacc
    10021 tgtctctcga ccctttccct aggatagact tggaaaaaga taaggggaga aaacaaatgc
    10081 aaacgaggcc agaaagattt tggctgggca ttccttccgc tagcttttat tgggatcccc
    10141 tagtttgtga taggcctttt agctacatct gccaatccat ctcattttca cacacacaca
    10201 caccactttc cttctggtca gtgggcacat gtccagcctc aagtttatat caccaccccc
    10261 aatgcccaac acttgtatgg ccttgggcgg gtcatccccc cccccacccc cagtatctgc
    10321 aacctcaagc tagcttgggt gcgttggttg tggataagta gctagactcc agcaaccagt
    10381 aacctctgcc ctttctcctc catgacaacc aggctcccag gtcccgaaaa ccaaagaaga
    10441 agaacgcaga tctctagagt caccgagttg ctttaccgga tgaagagggc cgaaacatac
    10501 tgtccaaggc ccttgctggc aatccaccca actgaagcca gacacaaaca gaaaattgtg
    10561 gcaccggtga aacagacttt gaattttgac cttctcaagt tggcgggaga cgtcgagtcc
    10621 aaccccggac cggtgcatgt ggaccccaag aaaaagcgga aggtggaccc taaaaagaaa
    10681 cgcaaagtgg atcctaagaa aaaaagaaag gtgcacgtgt ccatggtgtc caagggcgag
    10741 gaactgttca ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac
    10801 aagttcagcg tgtccggcga gggcgaaggc gacgccacat acggaaagct gaccctgaag
    10861 ttcatctgca ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc
    10921 tacggcgtgc agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag
    10981 agcgccatgc ccgagggcta cgtgcaggaa agaaccatct tctttaagga cgacggcaac
    11041 tacaagacca gggccgaagt gaagttcgag ggcgacaccc tcgtgaacag aatcgagctg
    11101 aagggcatcg acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac
    11161 aacagccaca acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc
    11221 aagatccggc acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac
    11281 acccctatcg gcgacggccc tgtgctgctg cctgacaacc actacctgag cacccagagc
    11341 gccctgagca aggaccccaa cgagaagagg gaccacatgg tgctgctgga attcgtgacc
    11401 gccgctggca tcaccctggg catggacgag ctgtacaagc ctgcaggcga gggcagagga
    11461 agtcttctaa catgcggtga cgtggaggag aatcccgggc cgtccaattt actgaccgta
    11521 caccaaaatt tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg
    11581 atggacatgt tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc
    11641 gtttgccggt cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa
    11701 cctgaagatg ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtaaaaact
    11761 atccagcaac atttgggcca gctaaacatg cttcatcgtc ggtctgggct gccacgacca
    11821 agtgacagca atgctgtttc actggttatg cggcggatcc gaaaagaaaa cgttgatgcc
    11881 ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca
    11941 ctcatggaaa atagcgaccg ctgccaggat ataaggaatc tggcatttct ggggattgct
    12001 tataacaccc tgttacgtat agccgaaatt gccaggatca gggttaaaga tatctcacgt
    12061 actgacggtg ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca
    12121 ggtgtagaga aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgtc
    12181 tctggtgtag ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt
    12241 gccgcgccat ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca
    12301 actcatcgat tgatttacgg cgctaaggat gactctggtc agagatacct ggcctggtct
    12361 ggacacagtg cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg
    12421 gagatcatgc aagctggtgg ctggaccaat gtaaatattg tcatgaacta tatccgtaac
    12481 ctggatagtg aaacaggggc aatggtgcgc ctgctggaag atggcgatct cgagccatct
    12541 gctggagaca tgagagctgc caacctttgg ccaagcccgc tcatgatcaa acgctctaag
    12601 aagaacagcc tggccttgtc cctgacggcc gaccagatgg tcagtgcctt gttggatgct
    12661 gagcccccca tactctattc cgagtatgat cctaccagac ccttcagtga agcttcgatg
    12721 atgggcttac tgaccaacct ggcagacagg gagctggttc acatgatcaa ctgggcgaag
    12781 agggtgccag gctttgtgga tttgaccctc catgatcagg tccaccttct agaatgtgcc
    12841 tggctagaga tcctgatgat tggtctcgtc tggcgctcca tggagcaccc agtgaagcta
    12901 ctgtttgctc ctaacttgct cttggacagg aaccagggaa aatgtgtaga gggcatggtg
    12961 gagatcttcg acatgctgct ggctacatca tctcggttcc gcatgatgaa tctgcaggga
    13021 gaggagtttg tgtgcctcaa atctattatt ttgcttaatt ctggagtgta cacatttctg
    13081 tccagcaccc tgaagtctct ggaagagaag gaccatatcc accgagtcct ggacaagatc
    13141 acagacactt tgatccacct gatggccaag gcaggcctga ccctgcagca gcagcaccag
    13201 cggctggccc agctcctcct catcctctcc cacatcaggc acatgagtaa caaaggcatg
    13261 gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc tctatgacct gctgctggag
    13321 gcggcggacg cccaccgcct acatgcgccc actagccgtg gaggggcatc cgtggaggag
    13381 acggaccaaa gccacttggc cactgcgggc tctacttcat cgcattcctt gcaaaagtat
    13441 tacatcacgg gggaggcaga gggtttccct gccacagctt gatgaagatc tgagctctta
    13501 attaatgatc ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct
    13561 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
    13621 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    13681 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    13741 ctggatccgt ttaaactaac tttaaataat tggcattatt taaagttaca tatggcggcc
    13801 gccctaaggc tcgacctcga aattctaccg ggtaggggag gcgcttttcc caaggcagtc
    13861 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
    13921 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
    13981 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
    14041 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
    14101 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
    14161 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
    14221 caggggcggg gcgggcgccc gaaggtcttc cggaggcccg gcattctgca cgcttcaaaa
    14281 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcatcca
    14341 tctagatctc gagcagctga agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
    14401 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
    14461 cgccacgcgc cacaccgtcg atccagatcg ccacatcgag cgggtcaccg agctgcaaga
    14521 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
    14581 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
    14641 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
    14701 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
    14761 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
    14821 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
    14881 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
    14941 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
    15001 aaggagcgca cgaccccatg catcgatgat atcagatccc cgggatgcag aaattgatga
    15061 tctattaaac aataaagatg tccactaaaa tggaagtttt tcctgtcata ctttgttaag
    15121 aagggtgaga acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg
    15181 tggggtggga ttagataaat gcntgctctt tactgaaggc tctttactat tgctttatga
    15241 taatgtttca tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc
    15301 attcctccca ctcatgatct atagatctat agatctctcg tgggatcatt gtttttctct
    15361 tgattcccac tttgtggttc taagtactgt ggtttccaaa tgtgtcagtt tcatagcctg
    15421 aagaacgaga tcagcagcct ctgttccaca tacacttcat tctcagtatt gttttgccaa
    15481 gttctaattc catcagaagc tggtcgagta actttaaata attggcatta tttaaagtta
    15541 ggcgcgccat aacttcgtat agcatacatt atacgaagtt atgtcgagat atttaaatat
    15601 tagacccagc tttcttgtac aaagtggttg atatctctat agtcgcagta ggcggagtat
    15661 tggattttat ctggtcttct cttttgtctt tgatgtagtt aataactctt agactctctc
    15721 tgagagctta ggccaaaacc cttctccctt tacatttgag aaataaagaa aaactgaaca
    15781 ccatagtgaa tactgttgta tccagttcag gaccaaggct aggacttagc tgcttctcac
    15841 cttatcaggt cctgtgcaca aaaccgctgg ctcctccccc tcccatcagg tcaccagtgt
    15901 gatggctgac tattgccact gccttcatct ttctttgtca tgtgaccaga gctgccttgg
    15961 cttttttttt tccccctgga gggtctgaat caggggtggg atgggggatg ctcttcagtc
    16021 ttctatgtaa ggctgatagc accatttacc taaccctttg gattcctttg cagattatgt
    16081 ttgagacctt caatgtcccc gccatgtatg tggctattca ggctgtgctg tccctctatg
    16141 cctctggacg tacaactggt aggtggccgg ggaggtgggg accttgatca aatgaatcca
    16201 tcgtaaagtg tgatatttga tgggtacaac ctgtgggttg tgatggttga caagtgaatt
    16261 ggagcacaca gagatgtggt cctcccccag agcccgcaaa aaggaaagaa aaagccctcg
    16321 ccttttcaaa accagccctg gacctcacca cacttcttta cttctccagc aataaatttt
    16381 taatggcttc tccctcttct ttctgtctct cccaagccct agctctgacc tatcccatag
    16441 agcacattct ttcttccctt gatgtaaggc tcttaggact caccactcct ctcagcctcc
    16501 gtgtgaaaga cagaaagtaa ctgagcacct gctgccgtgg tgacttacag catacttaat
    16561 tgttccccag gtattgtgct ggactctgga gatggtgtga ctcacaacgt gcctatctat
    16621 gagggctatg ccctgcctca tgccatcatg cgtctggact tggctggccg agatctcacc
    16681 gactacctca tgaagatcct gactgagcgt ggctattcct tcgtgactac tggtgaggct
    16741 cgggggtggg gggaggtgga gggaagagga tggaagacac tatgttgctc actgtaacct
    16801 atcagaagga gggctgtccc catgagaatg gggacataaa gccagagagg agacttcgct
    16861 gataaaacca tttgctgtgc aaaccttaca acctacggtc agttcctgag ctcacagcga
    16921 aaggcaagga ctgacggctg agatttgctt tctgatgtcc acacatacac acctgaaatg
    16981 cacacacaca cacacacaca cacacacaca caaacacaca taaacacacc caagcacaca
    17041 ctcctatgca cacccatttg cacacacatg catacacata caaataataa tattaacatg
    17101 ctaaaattaa aaaaataaaa ataaaataat aaaaaagggt tggtatgtca gagacttagc
    17161 caacagtaaa gcctgacccc aatgcacaga gaaagcttag ctttccttgc tttgtgattg
    17221 taaagctatt acagtttctt tgagagtagc tatagacatc agtgtaggcg gcaagtcaaa
    17281 gtggaaaatc gtttttgtct atactgctgg agatcgaacc cagggtcttg tattggctag
    17341 ccaagccctc taccacccag ctacacccca cagaactacg tggataattt tttaatttct
    17401 tcatttacaa atagaagaga acgtgtacaa tagagagagg tatagctggg acctttccca
    17461 tctctccgtg tgtaccaatg gaactggggc tgttcccaga gagcatctgc ttcattttga
    17521 aaagtgaaaa atgtgtcaaa gatcaagcta ctgccgatct gggtagggca gcaaagatct
    17581 tttaggcatt tttgaaaact ctgagacttc tgagaagctg gttcagtaga agagtcacat
    17641 gggacaggta gattttagcg cctgtatcaa ccctaatgtg tatcatgttc gttatgtgtt
    17701 taacagccga gcgtgagatt gtccgtgaca tcaaggagaa gctgtgctat gtagctctgg
    17761 actttgaaaa tgagatggcc acggccgcct cctcttcctc cctggagaag agctacgaac
    17821 tgcctgacgg gcaggtgatc accattggaa acgaacgctt ccgctgccca gagactctct
    17881 tccagccatc tttcattggt aggcaatgga gagtggggtc tccctgttct gtaccaagaa
    17941 caagccaagc ctctgctatc agggtccatt agcaggtaaa aatgaaccag ggcttcagaa
    18001 acagaagcag ttctaaagta gggatggtgt cacatttcca aaatgagaag agatggcgca
    18061 acgcaattaa tgataacttc gtatagcata cattatacga agttatggtc tgagctcgcc
    18121 atcagttcat acgtttacga tagtggaaca caggggtggt tggggaagac gatctgagtg
    18181 ctgagagttt ggggttgctg agagagaagg aaaacagaac aagtagagag cgtctattcc
    18241 acactggcac ggcagggtca cgttgggtct gcagaggcat cctctttgga ggctgggggc
    18301 ctgggcttga aactctgttc tattttcatg atctcgagaa tgtcactaaa gctttctatg
    18361 agtcagtttc ttcataccag gacagttgga agttttatag cagagttgtg cggtgccctc
    18421 agcacagggc ctgtccaaaa taagctttca acaattggtt actgccagca atgtaaaact
    18481 agctggtggc attgctatta taactagatc acaatgttaa gaaggccgtg ggcgccatct
    18541 agtggtatat tttaaaaagt ctatgtcaga agtcaacaag aaactccacc cctcgcctct
    18601 accccttaaa aaaaaagctg tacaaagatt gcatagaaag catctttggc agctgggcat
    18661 ggtagtacat gcctttaatt ccagtactag ggaagcaaag gcaagcgaat ctctatgagt
    18721 ttgaggccag tttgttctat atagcaagtt caggccagcc aaggacctgt gtttaaaaca
    18781 aaaccaaaac tgaaaacaaa caaaacactt tatccaagta ggagagacac tttactgaca
    18841 gttgcaccat tcaaacttgg gtgttgggaa cataaaggaa caatggcaga gagatgaacc
    18901 tgctcagacc catggccctg gccctacctc ttaccaggca tcggaaggcc tggctcctta
    18961 ctcaagccaa ttatagcaga atatctatcc agccactggc gttatcatgt tgcaatctca
    19021 ccccagaatc gctgcagctg aacctcctcc tttagacaag agccaggagg atggtatgga
    19081 gcacaagaag ggcctttagt ctcttgagtg gtagtttcta gttcatgatg cctgccagcc
    19141 tcacccagga gatgtggtag tttctagttc atgatgcctg ccagcctcac ccaggagatg
    19201 accacgtagc tgggccaact tgattttcca tgtgtagctt gagttttacc cagcatccaa
    19261 ggatcactgc ctgtgtctct tttccaggga tggagtcagc gggcatccac gaaaccacct
    19321 ataacagcat catgaagtgt gatattgaca tcaggaagga tctctatgct aacaacgtcc
    19381 tgtcaggggg taccaccatg tacccaggca ttgctgacag gatgcagaag gagatcacag
    19441 ccctcgcacc cagcaccatg aagatcaagg taccacacta agaccctctg actcagaaag
    19501 cagcaggagg ggcatttcta gaacagccac aagctaagag gcagtttaac aggctctctg
    19561 ttctttccaa agggaatgag gccaaggtga cagtgggtta gattttaaca ctaccactta
    19621 aaatgggccc tgaggagtcc tgtcctgggt ctatagggag tgagccactg cttcctgaag
    19681 agcctgtgct ccttcctgtg catgtcagag cagctcaagt ccccctcctt ccatacctag
    19741 ctgagcatgc tgccaactct atgtacttgg ggtttgagaa cagacaggct gcatctagta
    19801 ggagttaggc aagcctcaat tctccaaacc ttacttctga gatggaaatg aatgtgaaac
    19861 tcaggggtcc agtatggtca gagaacacag acacccagta aaagctcagg tcaccaagat
    19921 ggtacattca taaaatgagc ctgggtacaa cgttctggaa atctaaaatt atcaccaaac
    19981 cccctcacta tggaaaaaca aaaatgtgta ccttcaaagc aaaactcctc tgagggccag
    20041 ttttgaaatt aaacctgcta ttgatacagc cactagcgag catcactgac agtcccctga
    20101 gccagtatct tcatctgcaa cttgaacatc actggatacc tacccttcag aattattata
    20161 gaagttaagt aagctagaac ctgtcgaacc cttggcacag ggcacagtga gagcttggtg
    20221 aaggaggaaa gattaaatgt tgaaccagag cttgcagaca gagacaaagt aagcccctgg
    20281 ctagcatgtg caaacacttt atttactaga gcatcctctg ctactcaaga aactaagact
    20341 agagtggaat ttggtgttgc ttggctctat gtttgagttg ctgcaacaga gaaatccctt
    20401 gtctgatact ctgcacaacg cctccgccat tcactcgtac aaaacacaaa acaaaacaaa
    20461 aagtgggcat gatctggtat gtaagatctg tactctgaat aaggccagca tgacaggaga
    20521 tacaccggaa tgggaactct gagccctctg tgttgggcgg tgcttatggt caggacatca
    20581 gaaagcagtg gccagtttaa tcaggtggaa gattacttgc acagaaagtt caggaacaat
    20641 cgtctttgca cacagtagga cttctgtaga acgtgcagcg cctgctgagg tcagctctgg
    20701 aatcaagaga ttttgtcagg gtttttacaa aggggccccg tgtgggctct agggccagtt
    20761 tgggtttgcc caataaaggg ataaaggaat tagggatggt ttggcataga tttcataggg
    20821 ggaaaaaaac agaaaaatca atgggctcac tggggaaaca ctccaacttc cacaaaccca
    20881 tcccctcata gagaacaatg tcctcgtttt ccaggaaggg acagcacaag gcacgtctgc
    20941 taggcactaa tattcttccc ttcttaccct gacagatcat tgcccctcca gaacgcaagt
    21001 actctgtctg gatcggtggc tccatcctgg cttcgctgtc taccttccag cagatgtgga
    21061 tcagcaaaca ggaatacgac gaagctgggc cctccatcgt ccaccgcaaa tgcttctaag
    21121 tcccccctgc tctgcctcta gcacacaact gtgaacgttt tgtggatcag cgcctccagt
    21181 tcctttccaa atcattcctg cccaaagctt tgatttgtta ctcgtggttt ttttaaaaat
    21241 aaatcagaca tgtgctaccc ttaacttgta gccctggcct agcaacactg attcaattgt
    21301 gtttctgtta ggtgagaatc atttattata gggtggcccc ctctagtggt caggaataga
    21361 tgtgacacaa ctagaaaagt atattcccag gcatggatgg catcaatcac ttcaacagag
    21421 gaaggtccac ttaaatgatg taaacggggt atttgagagc gtataggact tgtatagatt
    21481 acatataata atatatatta gtaccaatag gaaaaatcga cactgacttc acagctagag
    21541 tgactatttt actttataca taca
//