LOCUS       82359#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21093 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 82359
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000040110
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..10011
                     /note="5 arm"
     misc_feature    10039..10063
                     /note="B1 Gateway"
     misc_feature    10076..10123
                     /note="Frt"
     misc_feature    10124..10623
                     /note="mouse En2 intron"
     primer          complement(10192..10213)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10088..10111)
                     /note="FRT3"
     primer          14289..14307
                     /note="seq primer NF"
     misc_feature    14349..14356
                     /note="RE sites"
     misc_feature    16149..16182
                     /note="loxP"
     misc_feature    complement(14357..14388)
                     /note="Rox"
     misc_feature    complement(16109..16140)
                     /note="Rox"
     misc_feature    14389..14410
                     /note="polylinker"
     CDS             14969..15565
                     /note="puromycin N-acetyl transferase"
     misc_feature    15084..15089
                     /note="Mutated RsrII site"
     intron          10624..10998
                     /note="mouse En2 intron"
     misc_feature    10996..10998
                     /note="Splice Acceptor"
     exon            10999..11052
                     /note="mouse En2 exon"
     primer          10899..10920
                     /note="seq primer FCHK2"
     misc_feature    11053..11175
                     /note="1Dleader"
     misc_feature    11176..11227
                     /note="F2A"
     misc_feature    11323..12039
                     /note="EGFP"
     misc_feature    11233..11322
                     /note="3X NLS"
     misc_feature    14105..14343
                     /note="SV40 polyadenylation site"
     misc_feature    13138..14082
                     /note="ERT2"
     promoter        14421..14932
                     /note="PGK promoter"
     misc_feature    15676..16108
                     /note="PGK pA"
     primer          14163..14186
                     /note="LRPCR RAF5"
     misc_feature    12049..12102
                     /note="T2A"
     misc_feature    12099..12099
                     /note="A to G to kill RsRII site"
     misc_feature    12103..13128
                     /note="Cre"
     misc_feature    11014..11053
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    11014..11029
                     /note="FCHK QC prefix region"
     misc_feature    11030..11047
                     /note="FCHK QC critical region"
     misc_feature    11048..11053
                     /note="FCHK QC suffix region"
     misc_feature    16205..16225
                     /note="B2 Gateway"
     misc_feature    10012..16255
                     /note="Synthetic Cassette"
     exon            16870..16955
                     /db_xref="ENSEMBL:ENSMUSE00000295464"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000295464"
     misc_feature    16256..17365
                     /note="Critical Region"
     misc_feature    17389..17422
                     /note="Downstream LoxP"
     misc_feature    17366..17445
                     /note="synthetic loxP region"
     misc_feature    17446..21093
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaaatttc cgagtttttg tgtcttagcc cattctggct tccataacaa
     4321 ccaaccacag actgtttagc atctaaacag aaatgtattt cttacaagtt caaggtactc
     4381 acaaattagt ctgcagaaca tctgctacct gtttcatggg gttctgtgtt ttctgtgtcc
     4441 tcctgaggta agataccatt tttatccaga gaggaacccc cattctaaag gctctgtccc
     4501 catggctgac acctcccaga ctttcagtat aggaatctca gggtgacaca aacattcttt
     4561 ccgttgttcc ttctactctc ccagagtggc tccatcacac ccagttctga agtctcagtc
     4621 tctctctctc tctctctctc tctctctctc tctctctctc tctctctctc tctccccctc
     4681 tctttgccat gcatatccac cccctgtgcc tgcacgaagc catctcacca cagtctctga
     4741 gacgccctcc ttgtccccca tgagcttgac ttaaggcttt aacaggtggt ttccttaggc
     4801 aggaccttgt gcttttggag gtagacacag ccccccaact tggtcatatt cctgcagaga
     4861 gaccagaacc ctcaggactg ggagaggcgg ttgtggaagc cgttggttct gatgaatcca
     4921 ttcagtggcc acttttatct ggcaggtgaa gttttgagcg agcaagtctg ttcttccatg
     4981 ctgggtaacg gggcccctgg ggaaggcttc ccagtcagtg agcctcctgg ttcttagatg
     5041 agtgctattg cttgtcttcc tcataatgct aggaatattt actgaattct caggtttata
     5101 cccttggcct tcagcagctg agagttctat tcagcctggt cctgcagggt atcaggaagg
     5161 actccttgcc ttgtgagctt aaatactaat ggacaccggt agggaattgc ctggaagagt
     5221 caggtttctc ccagcctatg cagccggaag agagaaggga atgggcttca tgcttacgag
     5281 tcggctgagg agtccactgg acatacacca ctacatgtac tggggaacat gccatcgtgt
     5341 attaaccttt ctcagtcatc tactgtcccc ataagaactc gggcgggagt tctagccaca
     5401 gctcctagac agtttctcat atagcagacc agggaactgc gttcttcctt gacgtccagt
     5461 gcctcccctt ccggtgtttc cccttccggt acctcctctt ccggtgcctc ccactccctc
     5521 ccttctccct atcctctact tttgcctgtt tgttttaggt ctccccaaac tctctctctc
     5581 tctctctctc tctctctctc tctctctctc tctctgtgtg tgtgtgtgtg tgtgcgtgtg
     5641 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tacatgtatg cacttgtgca ggtggcatgg
     5701 aagccagagg ggattctcca aagccctctt catcctttga gacaaggtct ctcataggcc
     5761 tggaggttac tcattcggtt agattgtctg gcagaggatc ccctccctgg atcctcctgt
     5821 ccccgcttct gagctgatta caggagtgta aggacacgcc tgacattttc atgtagattc
     5881 tggatatcca actcaggttc ttaaacccac tcttacctcc ctagcgctgt cttaatttaa
     5941 ctctgttgcc atcttgaagg agaactgccc tgtgaggatg agtctgttat caatctcact
     6001 aacttttctg cctcattctg aactactagt cagtggtttg gcgtcactcc ctcctgaaga
     6061 atcgatgctg ttggttaaac cctcagatag tctggcctct tgtctcactt tagctcacac
     6121 cacacatatg tactaagaac cccctgtgga caaagagggg cagtgacatt ggatgtgaga
     6181 cctggtggcc cgtgccaact ctgtagcccc ctctcccctc atgtaacagg ccattctgtc
     6241 cctccactgg cccaccttta ttctttgaga gtgaccaaaa gtgaccagat ggcatccaag
     6301 agcccctcag actctgaaca aattggattt gaagcaccaa agattctttg agataagatg
     6361 taggctccca gtcaaggggc cctggggacg tttatactat cgatggccta ggtacggatg
     6421 tggattgatg gagtctgttt accatgcatg ctcacgggaa ctgcttaaga gggcagggtc
     6481 catttggata tttgcatagg ctctatcagc cagtctatca gtcccttgaa aattaattag
     6541 ctaactaagt gcgtgtgtgt gcatgtgtat gtttgattat gcgggcatgt gaacatcaaa
     6601 ggacagatag caagcgtcct tcctctcctt ccacagtgca gtgtcccaaa gatcagactc
     6661 acatcatcac tggtacccac caagccgtct tgctgagccg gttgctcatt cttaaatgca
     6721 aaagtctgaa atctgaaaat tgcaactgtg gatcatttcg agccctggga tgctcctggt
     6781 tttgtgttct ccacgccttc tgcacagggc aggcttgttg ttcaacttat aaatagcacg
     6841 caagcccctt tggcttttgt ggcctggggt gccaaagtca gttcacatgg cacccccatg
     6901 aagtcacagg taaaggacct gaagctgcag gccccacacg gtccaagctt cttaaagacc
     6961 ttgttttaga aaaataaacc aattaaataa tattgcctcc tgtaaaagca ggcttttaat
     7021 gggttccacg gtgtggcaga tcccggtgag cttgtctgct gcctgctgcc gtggggagac
     7081 ttgagccttg cacagcgtgg gtgtaatttc acatggttta tggccatgtt cgcccctcca
     7141 agtgctgcga taccattata cagaaggagg acctgcatgc cctcaacacc ttacctcctt
     7201 tttggtggaa atgaaaaatg aagtcataaa ttaaagaaaa acacaaagaa cttttccatc
     7261 accttcctgg ggcagcctct gagggacaaa gaagcagtgt tttcctggat agcctaatca
     7321 agttctaagg gcttttaggt gttgactttt gctcaaagat ccctcctccc atgggaagac
     7381 ctctgggctt gcaggatctg tgcacagctt ctgttggagt cctgggctcc agggcagggg
     7441 atctctactc cagcctcagg gacaaatcct gcctcctgcc tatttttgtt aagccatcaa
     7501 agcaagccaa caaaggaagc atatttccta atgcaggaat gtcagattcc tgtccataag
     7561 caaagctcgc ttgctcacta gcatgcctca tgtcactctt cttctattgt tctttgaccc
     7621 agtggtggca cttagctaaa cactgcttgt cctttccaga aaatgcttgc tagccttctg
     7681 tttggaaggc aaggaaaggg ggtgggacat gtggcctttt ttcactggga tggatgcttt
     7741 tctttcaggg tctccacaag gctcaaaggg aggagcccgg gagggaggca gctcacctgt
     7801 tgtttatgct aactactctt tactgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     7861 gtggaggggg agaggtgtgt atgtggaggg ggagtggtgt gtgtgtgtgt gtgtgtgtgt
     7921 gtgtgtgtag gaggaggagt aggagtaggg tgtgtgtgta tgtgaatgtg tgtatgggga
     7981 agtggtttgt gtgtgaatgt gtgtgtgtgt gaatgtgtgt gtgtgtgtgt gtgaatgtat
     8041 gtgtgtgtgt gtgtgtaggc ggttggttcc tgtgtgtgcc tatgtgcatg aatgtgtgtt
     8101 tatatgtgag tgtgtatgtg gaggggagtg gtttgtgtgt gtttgtgtgc gcatgtgtgg
     8161 aggggaggag agtgcttggt atgtgtgtgt gtttgcaaaa gccagagatt gatatcatgt
     8221 gacaccttat tttctgagac cagtcctctc attgaaccta gaattctgca cttcagttag
     8281 gttagttggc cagtgagctc caggggtctc atctctacgt tcccagggtg ggagaagtac
     8341 tcactagtgg gcagttgcca ctgtatctgg ttggttggtt taatgtgggt tctggggatc
     8401 tgaactcagg ccttcatgct tcctcaggaa tctatttgcc aaatgaacca tctcccaagc
     8461 cccaggctcc tcactctgtc ctcaggctga catatgagta cccccctccc ccactcccca
     8521 ttgcagcccc tttcctagag gcatcttagt cttttgactg tatcttgatt ggtgtgtggt
     8581 cagttctcac tcagcccact ccctagctag ctcaggcaga gaggtgttcc catctttcta
     8641 cctacagcca ggcaacaccc atcatacatt gctatgggca tacctagagt ggcttcagaa
     8701 acttctgttg ccaggcacat tgtaagcagt ctgcattgta actcaagctt cacaaccagc
     8761 ccagagagat tctcttccaa ctcccttata caggtgagga agcggacgga gaggatgcgg
     8821 ggtttgcctg aggtcacaca actggtaccg ggttagagcc atgatgaaac cccaggctgt
     8881 ctggtccagg gaataaggga atgtgcactt ggtccccact acacaccaga gtgctccctg
     8941 gacttctggg agtctctgct gttcttgctc cattaatctt cattacgatg atactctgta
     9001 acgatactct gtaacgaatg gtttggtgtt tgaacaaagc cctgttcatg tgttctaccc
     9061 gctgctcctt ctccctctcc ttttctcttc ttcccccttc tcatctcctc cctccctccc
     9121 ccctccctcc ctccttcttc cttccctccc tccttcctcc ctttttcctc cctcgctccc
     9181 tctcctctgt cctctcctct tcctctgtct ataattaggt gtccatgcat gggcttaggg
     9241 tgcttgtagg caggaaatat gaaaaaaggg ttcatttcta tggacaagga ttcttggccc
     9301 cctcacccca ggggaatgta gagttctaat cctcatagca gttgtaagtg ccggttcttt
     9361 ccagaagcac agaagcacag accttcatcc tttctgcctc aagttgctat tttaatgaaa
     9421 atgaacattg taaaagactt aggagagact gggcttttga cctacggtgg aggggccctt
     9481 gaaaatgatt tcccccattc ttaggagcgc aggtctcagg tgcctggagg aggtggtgtt
     9541 gcccgagggt ggtacctgca cgctgcgtga cccagtcagc acagaactgt gtgggatcag
     9601 gatggaggtg gaagtttctg cctcagcgaa acctgcgcca tagaaacgtc acagagctgc
     9661 tacagcagga tgctcccggg cccttggtgg cactggcata accacataca cagttctcta
     9721 aaagcaaagt gtttcccatc caggatatcc catgagacag tgtgaatagc tgtgttactg
     9781 gtttgtagtt gtttgctccc atttttatca tcagtgtaga atgcatttct tcctgtgaag
     9841 atgccgtaat ggctagtagt tgtcgtcagc ctgatatacc caaagagagg gagcctcatt
     9901 tgagggtttg cctccatcag gctggcctct atatatgtct gtgggacatt tgcttgattg
     9961 ctaattaatg gtggatggtg cctgagtggc tgtcttgggt ggtatgagaa aaaggcgcat
    10021 aacgatacca cgatatcaac aagtttgtac aaaaaagcag gctggcgccg gaaccgaagt
    10081 tcctattccg aagttcctat tctctagaaa gtataggaac ttcgaaccct ttcccacacc
    10141 accctccaca cttgccccaa acactgccaa ctatgtagga ggaaggggtt gggactaaca
    10201 gaagaacccg ttgtggggaa gctgttggga gggtcacttt atgttcttgc ccaaggtcag
    10261 ttgggtggcc tgcttctgat gaggtggtcc caaggtctgg ggtagaaggt gagagggaca
    10321 ggccaccaag gtcagccccc cccccctatc ccataggagc caggtccctc tcctggacag
    10381 gaagactgaa ggggagatgc cagagactca gtgaagcctg gggtacccta ttggagtcct
    10441 tcaaggaaac aaacttggcc tcaccaggcc tcagccttgg ctcctcctgg gaactctact
    10501 gcccttggga tccccttgta gttgtgggtt acataggaag ggggacggga ttccccttga
    10561 ctggctagcc tactcttttc ttcagtcttc tccatctcct ctcacctgtc tctcgaccct
    10621 ttccctagga tagacttgga aaaagataag gggagaaaac aaatgcaaac gaggccagaa
    10681 agattttggc tgggcattcc ttccgctagc ttttattggg atcccctagt ttgtgatagg
    10741 ccttttagct acatctgcca atccatctca ttttcacaca cacacacacc actttccttc
    10801 tggtcagtgg gcacatgtcc agcctcaagt ttatatcacc acccccaatg cccaacactt
    10861 gtatggcctt gggcgggtca tccccccccc cacccccagt atctgcaacc tcaagctagc
    10921 ttgggtgcgt tggttgtgga taagtagcta gactccagca accagtaacc tctgcccttt
    10981 ctcctccatg acaaccaggc tcccaggtcc cgaaaaccaa agaagaagaa cgcagatcgc
    11041 agatcgcaga tctctagagt caccgagttg ctttaccgga tgaagagggc cgaaacatac
    11101 tgtccaaggc ccttgctggc aatccaccca actgaagcca gacacaaaca gaaaattgtg
    11161 gcaccggtga aacagacttt gaattttgac cttctcaagt tggcgggaga cgtcgagtcc
    11221 aaccccggac cggtgcatgt ggaccccaag aaaaagcgga aggtggaccc taaaaagaaa
    11281 cgcaaagtgg atcctaagaa aaaaagaaag gtgcacgtgt ccatggtgtc caagggcgag
    11341 gaactgttca ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac
    11401 aagttcagcg tgtccggcga gggcgaaggc gacgccacat acggaaagct gaccctgaag
    11461 ttcatctgca ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc
    11521 tacggcgtgc agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag
    11581 agcgccatgc ccgagggcta cgtgcaggaa agaaccatct tctttaagga cgacggcaac
    11641 tacaagacca gggccgaagt gaagttcgag ggcgacaccc tcgtgaacag aatcgagctg
    11701 aagggcatcg acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac
    11761 aacagccaca acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc
    11821 aagatccggc acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac
    11881 acccctatcg gcgacggccc tgtgctgctg cctgacaacc actacctgag cacccagagc
    11941 gccctgagca aggaccccaa cgagaagagg gaccacatgg tgctgctgga attcgtgacc
    12001 gccgctggca tcaccctggg catggacgag ctgtacaagc ctgcaggcga gggcagagga
    12061 agtcttctaa catgcggtga cgtggaggag aatcccgggc cgtccaattt actgaccgta
    12121 caccaaaatt tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg
    12181 atggacatgt tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc
    12241 gtttgccggt cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa
    12301 cctgaagatg ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtaaaaact
    12361 atccagcaac atttgggcca gctaaacatg cttcatcgtc ggtctgggct gccacgacca
    12421 agtgacagca atgctgtttc actggttatg cggcggatcc gaaaagaaaa cgttgatgcc
    12481 ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca
    12541 ctcatggaaa atagcgaccg ctgccaggat ataaggaatc tggcatttct ggggattgct
    12601 tataacaccc tgttacgtat agccgaaatt gccaggatca gggttaaaga tatctcacgt
    12661 actgacggtg ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca
    12721 ggtgtagaga aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgtc
    12781 tctggtgtag ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt
    12841 gccgcgccat ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca
    12901 actcatcgat tgatttacgg cgctaaggat gactctggtc agagatacct ggcctggtct
    12961 ggacacagtg cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg
    13021 gagatcatgc aagctggtgg ctggaccaat gtaaatattg tcatgaacta tatccgtaac
    13081 ctggatagtg aaacaggggc aatggtgcgc ctgctggaag atggcgatct cgagccatct
    13141 gctggagaca tgagagctgc caacctttgg ccaagcccgc tcatgatcaa acgctctaag
    13201 aagaacagcc tggccttgtc cctgacggcc gaccagatgg tcagtgcctt gttggatgct
    13261 gagcccccca tactctattc cgagtatgat cctaccagac ccttcagtga agcttcgatg
    13321 atgggcttac tgaccaacct ggcagacagg gagctggttc acatgatcaa ctgggcgaag
    13381 agggtgccag gctttgtgga tttgaccctc catgatcagg tccaccttct agaatgtgcc
    13441 tggctagaga tcctgatgat tggtctcgtc tggcgctcca tggagcaccc agtgaagcta
    13501 ctgtttgctc ctaacttgct cttggacagg aaccagggaa aatgtgtaga gggcatggtg
    13561 gagatcttcg acatgctgct ggctacatca tctcggttcc gcatgatgaa tctgcaggga
    13621 gaggagtttg tgtgcctcaa atctattatt ttgcttaatt ctggagtgta cacatttctg
    13681 tccagcaccc tgaagtctct ggaagagaag gaccatatcc accgagtcct ggacaagatc
    13741 acagacactt tgatccacct gatggccaag gcaggcctga ccctgcagca gcagcaccag
    13801 cggctggccc agctcctcct catcctctcc cacatcaggc acatgagtaa caaaggcatg
    13861 gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc tctatgacct gctgctggag
    13921 gcggcggacg cccaccgcct acatgcgccc actagccgtg gaggggcatc cgtggaggag
    13981 acggaccaaa gccacttggc cactgcgggc tctacttcat cgcattcctt gcaaaagtat
    14041 tacatcacgg gggaggcaga gggtttccct gccacagctt gatgaagatc tgagctctta
    14101 attaatgatc ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct
    14161 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
    14221 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    14281 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    14341 ctggatccgt ttaaactaac tttaaataat tggcattatt taaagttaca tatggcggcc
    14401 gccctaaggc tcgacctcga aattctaccg ggtaggggag gcgcttttcc caaggcagtc
    14461 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
    14521 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
    14581 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
    14641 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
    14701 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
    14761 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
    14821 caggggcggg gcgggcgccc gaaggtcttc cggaggcccg gcattctgca cgcttcaaaa
    14881 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcatcca
    14941 tctagatctc gagcagctga agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
    15001 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
    15061 cgccacgcgc cacaccgtcg atccagatcg ccacatcgag cgggtcaccg agctgcaaga
    15121 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
    15181 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
    15241 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
    15301 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
    15361 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
    15421 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
    15481 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
    15541 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
    15601 aaggagcgca cgaccccatg catcgatgat atcagatccc cgggatgcag aaattgatga
    15661 tctattaaac aataaagatg tccactaaaa tggaagtttt tcctgtcata ctttgttaag
    15721 aagggtgaga acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg
    15781 tggggtggga ttagataaat gcntgctctt tactgaaggc tctttactat tgctttatga
    15841 taatgtttca tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc
    15901 attcctccca ctcatgatct atagatctat agatctctcg tgggatcatt gtttttctct
    15961 tgattcccac tttgtggttc taagtactgt ggtttccaaa tgtgtcagtt tcatagcctg
    16021 aagaacgaga tcagcagcct ctgttccaca tacacttcat tctcagtatt gttttgccaa
    16081 gttctaattc catcagaagc tggtcgagta actttaaata attggcatta tttaaagtta
    16141 ggcgcgccat aacttcgtat agcatacatt atacgaagtt atgtcgagat atttaaatat
    16201 tagacccagc tttcttgtac aaagtggttg atatctctat agtcgcagta ggcggggcag
    16261 tgtcccgtcg tgtgtcagta tctcttcctg ctctgtcttt cccttcacga tgaactataa
    16321 gctgtaaggt gaaataaacc cttccctttc caagttctga cccttgtcac agcaacagaa
    16381 agcaaactgg gactcatgct cacagtggca gtcttccgtg ccctcttcag cagcctcgtc
    16441 acaggacctc tgtgaacacc catctcgaat cttgtcagtg tatgtgactg tgtcacctca
    16501 cagtgaggtt ctcagaccac agccctgttg ccaggtgatg tgagactagg atcccacacc
    16561 ctggcacagc cacacgtgtt cccttgggta ctctgatgcc ttctgaaagt ggctacatcc
    16621 cagtgagctc tagatgcctg tcctgttttc ctgtgtgcgg cagaaagcat ttctagtgtg
    16681 gtctgtgccg tggggcgtgg ggacagaaag gcgtggtgct gttgtggcct ggacagcagt
    16741 gggctctttg ctagaagctg ctccttcctc tttgcatcct gtttcttcct ccctcccaga
    16801 tgcctttgcc tctgacagac agcaggcctt tatagctgtt ttctgatgag cttcactttt
    16861 tctccccagt catgcggagg aaccccttcg gtgtggacat ctgctgccgg aagggatcga
    16921 gaagtcccct gcaggagctc tacaatccca tccaggtagg accccggtcc tcgttgctat
    16981 cagtgtatgt tctggggatt tgacatgccc tttcctgcct tcatgttcag catcctttac
    17041 cggaaacatt cccaaactca aacccttgtg agcagcctca cggtaccaca agtggaaaat
    17101 cccgtgtctg atattatgcg atggatcaca gtcaaaatgc aggtgcactg aaatactgta
    17161 caaagttatc tcaggacact gtacataaga tgcatatgca atataaacaa atctcgtgtg
    17221 acatcgtccc aggtcctagc aatgggactc tgaacttcaa aagtctgcaa gcttgtgcaa
    17281 ctctccactg gtacccccag aaaggtgtgc acatggataa actccgagtg cagtgagtga
    17341 gacccagtga tgacctcgct acagagagat ggcgcaacgc aattaatgat aacttcgtat
    17401 agcatacatt atacgaagtt atggtctgag ctcgccatca gttcaaaagt agctgttgct
    17461 ggtttttttg actgattcgt ttaacaaggt ggcgagtgtg gcggttgggg tggggagtgg
    17521 actgtgcctt ccatgggaag tgaggagcag tcaggaggcc tgctcaccat cgcagtaacc
    17581 tcaggccact tgagttggtg tggagactct tgagcccgtc cctctgtcgg aatgtctgtt
    17641 agtgtcgctc ctgattgagg gaccgagcca atgcccacag accttggtaa ctgctgagaa
    17701 atgagtgcgt gctgaggaga gatggcctgc gcgtgatctc agggcttcac tttgtgctcc
    17761 atctcaatta tgtatttcca tagaacgtgg tgcctgaggg agaagataat atgctcggat
    17821 ctcagcgagg acagattctt agagtgattg caaaggcaca aacttcaaag cttcttggct
    17881 ccactttctc cctcgagcct tccacctgtg ctcccttctg ggggaaaaat aggactagaa
    17941 tccgggcacc cttggtgacc taatggtgtt cagtggcgcc ctgttgattc tctgtcttga
    18001 ggctctaggt gaggattgca aactcaatgc ctcttgaggc ttcctctgct ggcttttgca
    18061 gagagagact caagggggat ttgatatggg ttcctttgct gcccccgtga cctggccatt
    18121 gtgagtaagt ttcctagcct gtctactctg tcatctttat aaaaagatca agcaggcttt
    18181 ggccacacat agaatttctg atatgctcta ttgaaactca cccaggctac tccatcagaa
    18241 ccatccgcac tccctctgga catcctgtat tgatgaacag cacaagatgc aagatgcata
    18301 ggcagagcca cttctactgt agctgaccct ctgggtagaa agcccatgga ctgggtcttg
    18361 cctaccatat acatccggtg cctaacactg ggcctggttg atgtctgggg tccccaagta
    18421 tctgtgacat ttgaaagagc aaaagttcag ggttcaggtt ccctcttccc acgtctctag
    18481 ctgcgcaaac cggaaggtgc ctgcactagg tgcccgtttc ccagcatgcc tctggaactc
    18541 tggaggatga ctgtgaactg tcactttcct caatttcaga gtcagacttg gtgtcaaagt
    18601 ccagtgacat catttcctag ctctgtgacc ttgcacaggc cacataacca cgctttgact
    18661 gccatccctc ttttatgaca ttcggggact aataccaact gctgtattca agaggcggtg
    18721 cagtgacctt ctgtgctggc tgcctgctat ggggcttgtg ctttcctggt ttgttccctc
    18781 actgaccccc tggcatgtca gagactgggc atagagggca ctgaagctaa tatagccacc
    18841 tgcgatgggt acttgctctg tcccaggact ggtgtgcaca gacggccttg tctgtttttt
    18901 ttggcagacc agtcagagga aaggtgacta tcccatctcc tgtcatccct cctcacagcg
    18961 atagtgagtg ttgtgttagt gaaaggcaca ttcactaaca caaccagagg agggcactgg
    19021 taagtgagtc actatccttt aagggcatct tgggcacact gtctggcctc tccagcccat
    19081 caagcaaaca ctcttcagga agatcacccc atttcctgtg gatgtagttt tctgggtcta
    19141 gtttccttta caagtggcag ttgtgccccg tccccgcgca gtccttaagc agggtttgtt
    19201 tttgaagaag cagccctggt tagcggtggg ctgcggagtt taagatgggg acatgctgat
    19261 gagcttagac agcattgcct tcctgagtca tctccaggct taacgctggg ctgagctccc
    19321 ctgcacattc tcatagtttc ctaccagctc agctgccgag cctggaacag tgtagtcact
    19381 gaggcaagtg gcaaactctc tgatcgctct aacctgaacg gctcccagaa atggtgttcg
    19441 cagctttttt tttttttttt tttttttttt tttttttttt ttgctgagtg tctctttggt
    19501 cttagtaaca ggatggtaaa aatgagacca tttatttttt tatgacaaga aattcaccta
    19561 acagcaagaa gcaccttctg tttttgtttt tgttttttga gacagggttt ctctgtatag
    19621 ctcaggctgt cctggaactc actttgtaga ccaggctggc ctcgaactca aaaatacacc
    19681 tgcttctgcc tcccaagtgc tgggattaaa ggcgtgtgcc accatgctcg gctcaagaag
    19741 cgccttctta gttatctcgg atacataggc taggccagac acttcctggg tcttggattc
    19801 agctcactga atatgtatgg taagctctga gctagatggt atttcccctc ttatggatag
    19861 agaagtgaaa ctaataattg tatatgattt atttgagccc agatcactag taggaagcag
    19921 tgaggatcca accacagaag gaacggggaa attgcacgca gttactagtg actggtactg
    19981 gtgactatta ccctcgaaat cctcttggca ggaaccaacc ccctcctgca aacagatgta
    20041 tatccaacaa ggttttccct ggataaagag tgaaatcagc ccaggggcag ttgcctctac
    20101 tctgacttca gcatctgggg ctttcttagt gcactgaaac ccccaaacca tccaaagaac
    20161 aattggttaa gaaaaaaaaa tccttcccag tcgatattct tagcaaaata gccctgtaat
    20221 gtcttataat tttcctggtt ctctgctgtc tccctggccc caaaataaaa cataattgct
    20281 gattggatca gctggcgccg agtcaccgaa agttcaggag ttgccaacca cagacttgtg
    20341 ggccgagcca tctgcaggct gcccccgctc agtcagtgct gacccacagg gcagagaact
    20401 gctctcagct ggttgagagg gtgtaattgt ccctgaatgc catcgagggt gagacagctt
    20461 gtcctcctgc ctccccacct cccggcctct catggcttcc ctgaaatctt cacagtatca
    20521 ttcatgcggt ttggcctgcc ctgccatgtc tttctttagc atgtgtgcct gccctgttat
    20581 tagagcattc taagggggca gggtgcgtgg ctcagtgctg gccacgaact tgcaagcagc
    20641 atagctaggt ctgaacgggg cttcctggag tccctgggtc cagatgttta gggatctaga
    20701 agtgttctgg gtgatgtgtt gctgaatatg gctgaaactc tgcggtcctg ctccctcagt
    20761 tacggggcag tctcttggtg tgctgaagtg aactttctcc tccagggatg cactggatta
    20821 aggatggagt gaagaagaaa gggaggaagg agaaagacag ttggtgttta ctgaacatgg
    20881 aatacatatc tgtgactatt ctagaccttt tctaaacaag ggtcattgct cttgcttttg
    20941 ttgggttgga gatggaaccc aaggcccctt gaacactgag cagccatttt tcctaccagt
    21001 gacgcatgct ccaacccctc actgagcctt tacaagtccc ccttccagat gaaggtctct
    21061 tgataacctc acttttttag aaggtagcta agt
//