LOCUS       214270#pL1L2_GTK_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21477 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GTK_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 214270
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000029521
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            9438..9509
                     /db_xref="ENSEMBL:ENSMUSE00000174322"
                     /rank="1"
                     /note="ENSMUSE00000174322"
     misc_feature    4275..9987
                     /note="5 arm"
     misc_feature    10015..10039
                     /note="B1 Gateway"
     misc_feature    10052..10099
                     /note="Frt"
     misc_feature    10100..10600
                     /note="mouse En2 intron"
     primer          complement(10168..10189)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10064..10087)
                     /note="FRT3"
     primer          14103..14121
                     /note="seq primer NF"
     misc_feature    14163..14170
                     /note="RE sites"
     misc_feature    15963..15996
                     /note="loxP"
     misc_feature    complement(14171..14202)
                     /note="Rox"
     misc_feature    complement(15923..15954)
                     /note="Rox"
     misc_feature    14203..14224
                     /note="polylinker"
     CDS             14783..15379
                     /note="puromycin N-acetyl transferase"
     misc_feature    14898..14903
                     /note="Mutated RsrII site"
     misc_feature    11137..11853
                     /note="EGFP"
     misc_feature    11047..11136
                     /note="3X NLS"
     misc_feature    13919..14157
                     /note="SV40 polyadenylation site"
     misc_feature    12952..13896
                     /note="ERT2"
     promoter        14235..14746
                     /note="PGK promoter"
     misc_feature    15490..15922
                     /note="PGK pA"
     primer          13977..14000
                     /note="LRPCR RAF5"
     misc_feature    11863..11916
                     /note="T2A"
     misc_feature    11913..11913
                     /note="A to G to kill RsRII site"
     misc_feature    11917..12942
                     /note="Cre"
     misc_feature    10875..10896
                     /note="seq primer FCHK2"
     misc_feature    10601..11037
                     /note="mouse En2 exon"
     misc_feature    10989..11057
                     /note="FCHK cre_ert_gtk"
     misc_feature    10989..11024
                     /note="FCHK QC prefix region"
     misc_feature    11025..11037
                     /note="FCHK QC critical region"
     misc_feature    11038..11057
                     /note="FCHK QC suffix region"
     misc_feature    16019..16039
                     /note="B2 Gateway"
     misc_feature    9988..16069
                     /note="Synthetic Cassette"
     exon            16348..16993
                     /db_xref="ENSEMBL:ENSMUSE00000174323"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000174323"
     misc_feature    16070..17157
                     /note="Critical Region"
     misc_feature    17181..17214
                     /note="Downstream LoxP"
     misc_feature    17158..17237
                     /note="synthetic loxP region"
     misc_feature    17238..21477
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaaattta atagtaaaac cccttacata aataggatga ggaaagagtt
     4321 acaaagagtc aatgcaaaac acagatcact aaaactaata catagtaaaa attaaaggga
     4381 agaaaatagt aaaatatttg agaatgggat tacaagtgac attattttca tgagaatggt
     4441 ctttgtatac cctcattcac caatgacaca caaatacttt taatcttttg ttatcagaga
     4501 acacatgtga catcagaact gttgtgttat atgctaggca ttattccaag agcttaatga
     4561 tgattggctc aatctgcaaa tggccctttg agataagcat tccttcttca tctcatatag
     4621 ttttaggata caattataga tgaaatggga tctatagtga ttttgttatc tttacttctg
     4681 aaattccata gcttttcata catttttgtt tttaatttga tacttaaaaa cataaagatt
     4741 atgtgcattg atgggtgtca tttggcacat cagcacattg atgcagattc tttttacagt
     4801 ttcattgtgt gttcacgggc cgagctctgc tctgagatct ttatccgtat tgccttacct
     4861 agggagctag ggatggttat tagtccgatt ttacagagac aattaagcaa gttgcttgag
     4921 aatgcttggg tggagagagc aaagctggaa actggtcctg gaatgtgggt taagactgtg
     4981 catggtgttt cccactacag cccatccacc caggaatgga ccccagacac tgctgctctc
     5041 cccatctcca gttctttccc tgtacctttc attatgaact gtttacttac tgacagctct
     5101 cccagctcaa acaatgttgg tgacttccca ggggatttaa tctacccagg gagcattctt
     5161 tgtagggaaa atacgttttg tcttccatca taggtgcata aaagcttctt gaacaatccc
     5221 tcaaccccct tccccagcat tggaagaaaa gtctctacct tagatcatct atagaaaaga
     5281 atctctcggc cttccccttt gggatggttg ttatagctga agttccactt ctggcaactt
     5341 cagctgaatt ctctcctctc actttaccct ctatatacac atggtccctc tacatttggc
     5401 cagccagagg tctactcttt aggaccatat gtagaaatag ggccttcaat gatttctgcc
     5461 tacttggagg aatttatcaa tgtcaaatgt tcatggattt tttttggcat gtcaaattca
     5521 gacaagaaga gaatcccaac cacctctatc atcttcatta gacctacctg aaatagcatc
     5581 aatagaatat ccagcattct tggtcaaggt tatggcacca tctaagaatg aaaagagcca
     5641 cattagttca ttggcttggg cagggtttta ttttgggttc ttgtccatat tataaaaggg
     5701 ccatttaatt tcctctactt tctattctga agaagaaaca gatgttatct ttaccctctg
     5761 agctctagaa ttgaactggc tcatctcagg aaatagggac catagcagag ctgcttagga
     5821 gactgacaga caagaactgt atgtaatgtg caatgttcta tcaccatcct acaggcttgc
     5881 aattctcaga ggcttttctg tgcattaata cctcagatgc ttatagaggg aggaattaga
     5941 tctttgggat gtaaagggag ggagtcatac atacccctca tgaaagatgt tatcagtatc
     6001 ccacacatgg ggatctcagg tcagagcaga actgtataaa aggcaggcgg ttctcatagc
     6061 acctcaatcc attactcgct gagtgatcta ctccagtgcc tgaggcaggt agaacatctc
     6121 ccagaccaga gagactctga tgctagtttt ctgggcagaa aggggctctg gttggaactg
     6181 agggtaatct tgaagatgaa caaggggaaa agagggttag gagggaaggg tttctctgca
     6241 agtgaaaggt aatggaagat tgtactagta tctttttgac tttacagtga atttatttgg
     6301 aaggaagaag tgcttataca gggatcattt gtacttttta ttctcgaaat aaatttaaga
     6361 gattgggttt tcaaacttct aactatttta gagataagat actgtaaatt catagttcag
     6421 tcttcaaagc aacatttgag ctgttgacct tatatttttt tgtattattt ttcactttta
     6481 tccagttcct ttggagatgt cctgtcagca gagccagcaa cagtgccagc cccctcccaa
     6541 atgcaagacc ccaaagtgcc ctccaacctc ttcctgctgt agcctgggtt ctgggggctg
     6601 ctgtggttcc agctccgggg gctgctgcag ctctggatgt ggtggctgct gtctgagcca
     6661 ccacaagccc cgattctctc tccgtcgccg acgtcacagc tctggatgct gtagcagcgg
     6721 tggcagcagt tgctgtggca gcagcggtgg cagcagttgc tgtggcagca gcggtggcag
     6781 cagcggtggc agcagcggtg gcagcagcgg tggcagcagt tgctgtggca gcagcggtgg
     6841 cagcagcggt ggcagcagtt gctgtggcag cagcagcggt ggcagcagtt gctgtggcag
     6901 cagcagtggt ggcagtagcg gtggcagtag cggtggcagc agctgctgtg gtagcagtgg
     6961 gggcagcagt ggtggcagca gcagtggcag caacagccat cagcagtctg ggggctctag
     7021 ctgctgctct ggaggttgct gctgacctgg ccacgaagaa catggaaagc agcattgact
     7081 gaagacctgc aagcctaggg tctcctcttc tctggttttc ccgcagaagt acttagaact
     7141 ctctgtacgt cctcttcatc tccctaaagc tcatctacca cgaggtcttc aggtcccagc
     7201 tctgagatgc attggctttg ctttttttct ctgacacaac taattgagaa tgagatattg
     7261 aaaaattagc tttgaaaaat ctttttctgg gcaataataa agcttttgat tcctgacacc
     7321 atggcctccc aatgtacttt tgtttccctc ttccaattcc ctccctccac accaagtcat
     7381 agtccctttc gttatctaaa tcacacattt tgacactgaa tccacttaaa acttagtact
     7441 ctgaggcctg tgccccttat accaacttgc acagggggga caaggcatag gttaggacaa
     7501 gcagtaccca gaaaagctcc atgatgggac ggtgacactc aaaagggacc catgatttga
     7561 aaaagtttat ctcttaatca tggagaaaaa gattatctgg agtgcacatt gtgggctctc
     7621 atgagtagac acactgttgt ctgattatcg ccagctggcc aggcagggaa gctgagtgca
     7681 gagcatttct agagaagtaa gctgaggaga cagctgtgaa atgttgggag tccactattc
     7741 ttggaacctt agaattttag gtttgggttt gaatagagaa taaagaaacc atctcagcca
     7801 aaaattaccc ccgcctggtg gtagtggtgg tggtgcatgt gtgcatgcct gtgtatgtgt
     7861 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgatggagt caagggtact
     7921 taagtcactg agtttggatg ttcatgtaat attcctttag ggttgggatg gcttcctcag
     7981 gagatactaa aaatatttat gatagaatag ataactgcaa gtctgccttt ctatacaaga
     8041 taagcagtta gctcctcggt tgataagagt taacagtgat gcataaagtt ggggtaagga
     8101 gtccatacag tgagtatcta cttgatctct ccataatgat tcctatcacc ctggttttct
     8161 tcaccttttg cataattttc cagaggagaa cccacatcta acttagtact tcattacttc
     8221 cttctctttt ctaacattga tctctcttct tggaatgtct tacctcttct tgccaggtgc
     8281 tctggttcag taagcaacct gttaatcagg ctctaaagcc atggtaaatg aaatgggtat
     8341 cttctatgac ttgtctttcc ttgtctgttt cctcatgcca cacccagcaa tgcaccccaa
     8401 aacctatctc actcttaatc cctgaattct taatcttaga ttctcttatg tctcatactc
     8461 atggttggtg tgaccaggta gttccaatga ttgaatcaga ggcagttgac ctccaaagtt
     8521 tcctccagtt ttataaatca gctaatcgaa acgcaacaat tactatggga actttcaggc
     8581 cgttccccct tttattttcc tcctttttct tctttgctct tttattttat ccttttcatt
     8641 ttgttttgtt ttattttgtc tttttcctat ttgtcagtag ttctagggac aatctatcct
     8701 taaggagatg atatcagaaa caggagctcc ataggctaac cagcttacta ttttccaata
     8761 ggcctatact cctcaaccat ttttgtctgc gatccatcag ggttacataa tgggaatatg
     8821 gtcaagtgac ttcttttggt cttattggtc ccttaacctt gcctgggcta ttcttcctgt
     8881 taatggtgac aggaagaaac caccgttgtg atttccatcc ccaccatcat cttccttcag
     8941 tgctctctag aagcatctgg ataaaagcca gagggttcat cacatagcct gcagcctgct
     9001 atgaaagaca agaaccaagg gccatagaga tgcaaataag acaataaatg ataaggagat
     9061 agctatacat gaataagtgt gagaaagtag aacagtgcag gtgttctcct cccctcctgc
     9121 tgtgtgtgtg tatacagtgt ttaagagaga accccggacc tctcctcatt tgaccccccc
     9181 cccccaggcc ttctctaagc acatacagcc ctcccagcta tgaacagtat ccggtttgaa
     9241 aagtaaacac accacagcaa tcctggcacc tctgtgaagt gacttggttg tccacgtgca
     9301 ccacctacag tgctatctga gtgatgaatg cttctgggat gtaagtgtca gctgagcatg
     9361 tctgttgaga aatgggctcc tcccaaggac ctggacttgt tggaatctta taaaagcttc
     9421 tctggccata cactgttcag tcttctgcct agcaggtgtc aagttccagt tggagaactg
     9481 agtgagtctt cctgtggagc caaggtctag taagtatcta agcggggtgt ctgggggagg
     9541 aagctgggga gaatggaaga cagaaaggag gaagtaggat gcagccagaa tgtagctgtt
     9601 ggaatctggc ttccagtgag ccagcctttt gtgttgccta tgaagatgca aaggcttggc
     9661 atggcgctga cctttgaaag gttttccaga ctgtaaagag ctttgagtaa cccgggtctt
     9721 taggttccct ggttaagtta ttcctattct ctgccttttc cttataatgt gaaggacagg
     9781 caagaaagaa ggtgcagagc acgcttttgt gttttcgaat tctctatacc caagacagag
     9841 ggacacaggt aatacttgtg tgttttctga ccgatgtgat gcttatacga tattaactga
     9901 gattaccact tgaaagagct gcgtggtcaa agtacagtta ggaaaaggct gaagccaaga
     9961 gatttttgat tgagtcatat agataaaaag gcgcataacg ataccacgat atcaacaagt
    10021 ttgtacaaaa aagcaggctg gcgccggaac cgaagttcct attccgaagt tcctattctc
    10081 tagaaagtat aggaacttcg aaccctttcc cacaccaccc tccacacttg ccccaaacac
    10141 tgccaactat gtaggaggaa ggggttggga ctaacagaag aacccgttgt ggggaagctg
    10201 ttgggagggt cactttatgt tcttgcccaa ggtcagttgg gtggcctgct tctgatgagg
    10261 tggtcccaag gtctggggta gaaggtgaga gggacaggcc accaaggtca gccccccccc
    10321 cctatcccat aggagccagg tccctctcct ggacaggaag actgaagggg agatgccaga
    10381 gactcagtga agcctggggt accctattgg agtccttcaa ggaaacaaac ttggcctcac
    10441 caggcctcag ccttggctcc tcctgggaac tctactgccc ttgggatccc cttgtagttg
    10501 tgggttacat aggaaggggg acgggattcc ccttgactgg ctagcctact cttttcttca
    10561 gtcttctcca tctcctctca cctgtctctc gaccctttcc ctaggataga cttggaaaaa
    10621 gataagggga gaaaacaaat gcaaacgagg ccagaaagat tttggctggg cattccttcc
    10681 gctagctttt attgggatcc cctagtttgt gataggcctt ttagctacat ctgccaatcc
    10741 atctcatttt cacacacaca cacaccactt tccttctggt cagtgggcac atgtccagcc
    10801 tcaagtttat atcaccaccc ccaatgccca acacttgtat ggccttgggc gggtcatccc
    10861 cccccccacc cccagtatct gcaacctcaa gctagcttgg gtgcgttggt tgtggataag
    10921 tagctagact ccagcaacca gtaacctctg ccctttctcc tccatgacaa ccaggtccca
    10981 ggtcccgaaa accaaagaag aagaacgcag atcataactg actaggaggc caccatgggc
    11041 ggaccggtgc atgtggaccc caagaaaaag cggaaggtgg accctaaaaa gaaacgcaaa
    11101 gtggatccta agaaaaaaag aaaggtgcac gtgtccatgg tgtccaaggg cgaggaactg
    11161 ttcaccggcg tggtgcccat cctggtggaa ctggatggcg acgtgaacgg ccacaagttc
    11221 agcgtgtccg gcgagggcga aggcgacgcc acatacggaa agctgaccct gaagttcatc
    11281 tgcaccaccg gcaagctgcc cgtgccttgg cctaccctcg tgaccacact gacctacggc
    11341 gtgcagtgct tcagcagata ccccgaccat atgaagcagc acgacttctt caagagcgcc
    11401 atgcccgagg gctacgtgca ggaaagaacc atcttcttta aggacgacgg caactacaag
    11461 accagggccg aagtgaagtt cgagggcgac accctcgtga acagaatcga gctgaagggc
    11521 atcgacttca aagaggacgg caacatcctg ggccacaagc tggagtacaa ctacaacagc
    11581 cacaacgtgt acatcatggc cgacaagcag aaaaacggca tcaaagtgaa cttcaagatc
    11641 cggcacaaca tcgaggacgg ctccgtgcag ctggccgacc actaccagca gaacacccct
    11701 atcggcgacg gccctgtgct gctgcctgac aaccactacc tgagcaccca gagcgccctg
    11761 agcaaggacc ccaacgagaa gagggaccac atggtgctgc tggaattcgt gaccgccgct
    11821 ggcatcaccc tgggcatgga cgagctgtac aagcctgcag gcgagggcag aggaagtctt
    11881 ctaacatgcg gtgacgtgga ggagaatccc gggccgtcca atttactgac cgtacaccaa
    11941 aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac
    12001 atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct gtccgtttgc
    12061 cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc agaacctgaa
    12121 gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa aactatccag
    12181 caacatttgg gccagctaaa catgcttcat cgtcggtctg ggctgccacg accaagtgac
    12241 agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa
    12301 cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg ttcactcatg
    12361 gaaaatagcg accgctgcca ggatataagg aatctggcat ttctggggat tgcttataac
    12421 accctgttac gtatagccga aattgccagg atcagggtta aagatatctc acgtactgac
    12481 ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac cgcaggtgta
    12541 gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc cgtctctggt
    12601 gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg
    12661 ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga agcaactcat
    12721 cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg gtctggacac
    12781 agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat accggagatc
    12841 atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg taacctggat
    12901 agtgaaacag gggcaatggt gcgcctgctg gaagatggcg atctcgagcc atctgctgga
    12961 gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc taagaagaac
    13021 agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga tgctgagccc
    13081 cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc gatgatgggc
    13141 ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc gaagagggtg
    13201 ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg tgcctggcta
    13261 gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa gctactgttt
    13321 gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat ggtggagatc
    13381 ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca gggagaggag
    13441 tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt tctgtccagc
    13501 accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa gatcacagac
    13561 actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca ccagcggctg
    13621 gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg catggagcat
    13681 ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct ggaggcggcg
    13741 gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga ggagacggac
    13801 caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa gtattacatc
    13861 acgggggagg cagagggttt ccctgccaca gcttgatgaa gatctgagct cttaattaat
    13921 gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca
    13981 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
    14041 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    14101 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    14161 ccgtttaaac taactttaaa taattggcat tatttaaagt tacatatggc ggccgcccta
    14221 aggctcgacc tcgaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
    14281 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
    14341 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
    14401 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
    14461 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
    14521 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
    14581 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
    14641 cggggcgggc gcccgaaggt cttccggagg cccggcattc tgcacgcttc aaaagcgcac
    14701 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca tccatctaga
    14761 tctcgagcag ctgaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
    14821 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
    14881 gcgccacacc gtcgatccag atcgccacat cgagcgggtc accgagctgc aagaactctt
    14941 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
    15001 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
    15061 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
    15121 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
    15181 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
    15241 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
    15301 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
    15361 gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
    15421 cgcacgaccc catgcatcga tgatatcaga tccccgggat gcagaaattg atgatctatt
    15481 aaacaataaa gatgtccact aaaatggaag tttttcctgt catactttgt taagaagggt
    15541 gagaacagag tacctacatt ttgaatggaa ggattggagc tacgggggtg ggggtggggt
    15601 gggattagat aaatgcntgc tctttactga aggctcttta ctattgcttt atgataatgt
    15661 ttcatagttg gatatcataa tttaaacaag caaaaccaaa ttaagggcca gctcattcct
    15721 cccactcatg atctatagat ctatagatct ctcgtgggat cattgttttt ctcttgattc
    15781 ccactttgtg gttctaagta ctgtggtttc caaatgtgtc agtttcatag cctgaagaac
    15841 gagatcagca gcctctgttc cacatacact tcattctcag tattgttttg ccaagttcta
    15901 attccatcag aagctggtcg agtaacttta aataattggc attatttaaa gttaggcgcg
    15961 ccataacttc gtatagcata cattatacga agttatgtcg agatatttaa atattagacc
    16021 cagctttctt gtacaaagtg gttgatatct ctatagtcgc agtaggcggt atgctcttta
    16081 ttttccatca tggagctctt ggatggtcct aatgtcagtc agggagactg cactgatcct
    16141 tttcaaattc tgtttcctgc tttgtgcctc caccactgga agctcctgtt tgagtttgtt
    16201 tctttgtttc ccttcttttt ctttctttct tttattcctc ttgtcccaaa gccagacaga
    16261 cttttctcta aggcaaatac ttaaagttct gtgttccact atagggaaga aaaggcgggg
    16321 ttttaaaaaa gattgtcttt ctttcagatt catcttgaca aacgccgaga tgtctcaaca
    16381 gggcgccaaa ggcttttcca aagggtcttc ccagagttcc gccccctgcc ccgccccgac
    16441 tcctgccccc gcgtcctcct cctcctcttg ctgtggcgga ggctgctgca ggggctgcgg
    16501 gggcggctgc ggtggtgact ctggctgctg cggctccagt tccaccggct gctgctgctt
    16561 tcccaggaga cgccgccgac agcgctctgg cggctgcggc tgctgctgcg gaggaagcca
    16621 aaggtctcaa cgctcctgca acaatcagag ttcaggctgc tgctccggtg gctgctgaaa
    16681 gcctacagag cttctccgga acagctggca tcgcacagcg tctttgctgc tctcacccag
    16741 tcctccatag tagtaacagg aaggaccggc ctaggagttt ggtttccctt aagctaactg
    16801 cactttgatg ttcagaaact tcaccctgtt cttagacctg tcctctaacg ttgattttcc
    16861 tcccagggga tacaagtcag gggtgggact tcagatcacg ctatgggcag gaggttgtgc
    16921 acagcagtgt tcaaaacagg cctcctgatt tgattgtcgc ctggagacat aataataaag
    16981 cttctacctc ctatgaacgc ttcttttcct atttattcct ccatccgtgc cttcagtttt
    17041 cttattttat actacctacc cctcaaaggc tcatgcctga aggagaacaa acacattaac
    17101 tctctgcgag ttctcaggat ttggggtagg cagtctcatc tactttgtgt aatgactgag
    17161 atggcgcaac gcaattaatg ataacttcgt atagcataca ttatacgaag ttatggtctg
    17221 agctcgccat cagttcaaag attccggaga tcattttgtt aattggtgtg cttagcttgg
    17281 tctactcgaa gtgatttgga aactcggagt tcctcctaga gcaagccttg cccagtcccc
    17341 aggcccagac aaccgaggga gtctggtttg aaatggcaag tttaggaatt tgaattcagt
    17401 tctgttgaat gttttctctt cgttcctctt ttcttttcct cccttctttt ctacttctcc
    17461 cccccccctt ccctatcctt ttctcatttt tctttttctg gtggaaaaca attgactttt
    17521 gcttgctgga tgagaaaatt ttctatgctt accaggagcc tggaactcac catcttttat
    17581 gtctgatcta tgaagctgag gcatgaccag gaaccacagg actcctctgt ttgtggcagt
    17641 tggaacaaac tgtactagaa tagaagtctt tgtaagcata gagtttactg ttaaggttag
    17701 gtaagacctc ttagcctccc tcatgtcttt tagcttcaga agagattgta taataatgat
    17761 aaaaaaaata ggacaaaact agctaacatt tgttgaatat taattttgtt ccagacacaa
    17821 ggtatagcac agaatgtatg tatgtatatt catttaattt taataatctc aagaataggt
    17881 tttgattttc ctagttttaa aagtgtgagt ggattggggt atctgtgcca agtttcacag
    17941 gcagtgagtg tcctaggcct ctgatggtga atcatggggc taagacttaa actgagatct
    18001 acaggcattg aagtccacgg aatccactca gggccactga atccactcta ctgcaatcct
    18061 ggaaaccacc acttcctgaa agaaggggca cttgggcttc tatggacaca cccgagctct
    18121 tagaaaattg cagcttgact gctttgcatc agtgattgct gagctctttg tggcacctgc
    18181 ccagcagcca tctgctgaag tatttgttca gaggcaaagg aagaaatgca tcactgattc
    18241 atccatgctg tttaaagctg agactagatg acatcctatg ttggggatgg ttggaaggac
    18301 actcagtcct tctctgccac tcacttcggg ttctgagttc ggtgtttccg tgctttaatt
    18361 gcactgttta caaatagatg atctcattaa ggacctgcct cctccatacc agtcagtgac
    18421 gtgttgtgaa gcaacaggcc tcatgacttg ttggacagca aggcaggaag ggtgggcttg
    18481 cctgactcag aacatactgg aatggcagtg tgaggaacac agaatgcaag gacttgagaa
    18541 agagaaatgg taactcatta tgaaatatgt gagtctctag caatacatgt ctttgaccat
    18601 tgatagatca attggtcagt tgtgaggatg gcagcctcac tggttccctc ttatctgtga
    18661 aatacagaca tgtgggagat acgcaagaaa acatggttat ggattcttcc ttcccaagca
    18721 gcccaaggct tcaggtgttt aaggtatcgt tctggttata taggcagttg accattgtct
    18781 tcactaaaaa agaaacctgg gattccagct gcttggcata cagatttgct actaatatca
    18841 gtcttacact gtgaacttgg cactctttca tttactgctt aaaacactgc cacaagcgaa
    18901 gtgtttccat tgtttcttga taaggaaagg gaagcagaga ggcatcttaa gatgtcacaa
    18961 cttctaataa gaggacaaag ttttgaacag ggtgtttctg ataccaaaat catcattatg
    19021 gacacctttt tgtactatgc aattctaaga tggatgaagt cttctgatgg aagcaagaga
    19081 aagaatccaa ggtgttgctg gaattaaaaa atagatctct gaaaacatgt cctcagagga
    19141 gagaggagag gccaggctac aggtggctcc aaatgccaat tttaggaatt taacagtgtt
    19201 ggatttggca tagaaaaacg gtttctaaag aagaatttgg aaagacaaaa attgacctac
    19261 agaatatcca cagcaataat taggatgctc tggaaactta gaaagactat gtatcaatag
    19321 gcagctgcag tgacccaggc ctgagataag aatctgaaca tggcattggg caagggaagg
    19381 aaatgacagg tgagaaaagc atttgcctag agaaaaaaaa aatagagaga actgggagta
    19441 gcattggggt atatgaagac aaaccaatta aggtgtcatt gtggctttgt gcctgtcact
    19501 agaaaatgac agtaacattg acagagaagt ggagagtgaa agaagaggtg tgggtagaaa
    19561 ggggaatccc acccctaaca tgctggattt gaggcactgc cagagtatga acaaaaatca
    19621 gtgctgtgaa gcaaaaaaaa aaaaagcaga taatctttga aaattgattt gacaacatct
    19681 ggataaagag gaataaacta tgtggaggta ggaacccaat ctttggcatg aatgtagctt
    19741 cacttctgca atgaaattta tgaagaagaa gaagaagaag aagaagaaga agaagaagaa
    19801 gaagaagaag aagaaggagg aggaggagga ggaggaggag gaggaggagg aggaggagga
    19861 ggaagaggag gaggagggag aggaggagga ggaggaggag gaagaaaagg agaggaagaa
    19921 gaagaaagac ctggtatttc taactcatcc tagaaagctt gtgtaaaact agaaaattag
    19981 acactgaaca ctggatatgg cctgactgat atgtattgct tagtcaacct tggcaccata
    20041 agacacctaa ggtgaattga ggggaatgag caggaagaag tggaattcca aaagggggtc
    20101 aaagatgaag gcagaaaact cactcaggct ttccttgggc agctcatcag gctgctttca
    20161 agtcttgggt tctagaaatt caggctgaag aagagcagga cagagcaagg ttgcagaagg
    20221 aaaaggttgt cacctagtaa ggtcagtccc atggggcttc tttcccattg cttagtcaac
    20281 tctgtcacca ttagaaacag caagaagttc catttggagg gacagcagtt ccttcctcct
    20341 tgatttggta gaacttgtta aacaggcaaa ttggtatcac agtgagtaag gagaaaaatc
    20401 ctctctgctg tggatgacac tgcagcagac tcaaatgatt tggctgtgat aaatctctac
    20461 tctgtcagct tgcctcagga taaatggcag ccctagaaag gcaagtatca cctcctctct
    20521 tataaatact gaccagggtg aggacaaagg cttctaatct atcttattct gtgatgcatc
    20581 ttgttaagaa tgtcgttaga aggatgaggg agatggttca ctggaaaaga ggaacggtgt
    20641 tcaaatcccc tcctatgtaa aagaaaacaa aagcaaaatc tgggcatggc tgtgcattcc
    20701 tgtaacccca gcactgggga gcagaaacag gcagatcctg ggagagcttg ctagccagct
    20761 ggcttaggtg aaacggtaag cttccagttt tcaaggcagg aagaagaaag caataaagag
    20821 agtagacatc ttgctttgac ctctacattc atggatccat acatctgcat gttcatacat
    20881 ctgcatgtgt gcctcatata taccacagca ctcactcacc acagacagac agtgtgtgtc
    20941 tgttagtttt attgtcagtg atgagttgac aataaagctg tgttggatct acttatttgt
    21001 gaattgaatt tgcagtgttc aaagaccatt tttcttgctt ccaaccccca aaccactttt
    21061 ttttcccctg ccggtttgaa gtataattac atatacctga aaactagaag aaattaacct
    21121 ccttctgttt catgccctta tgtgattttc tgttcattca tcactgtaat ctttcattca
    21181 ttgaaaatgg attgattggt caccattccc ttcatactta atgatgtaaa aaaaaaagtt
    21241 aatggtgaga gttaaatagt agtgtgataa gtgcttaaag ccatcaccac atgacatcat
    21301 gactatacac aagtgacagt tcacactggt ctcaacggaa agagttgttt agacttaatg
    21361 ctggagatgg tgttggtcaa gctgagaaca tagaaaaaat atttaagtta aagaaaacag
    21421 caccaagggt gaagacagag agataaacac atattttata gcaaattcta aatgaaa
//