LOCUS       KO-first_condition_ready_121495_MGI:1919324        22332 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Anks4b targeting project(s): 121495
ACCESSION   unknown
SOURCE      Mus musculus.
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT2_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 48055
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..8522
                     /note="5 arm"
     misc_feature    8550..8574
                     /note="B1 Gateway"
     misc_feature    8587..8634
                     /note="Frt"
     misc_feature    8635..9134
                     /note="mouse En2 intron"
     primer          complement(8703..8724)
                     /note="LRPCR LAR3_EN2"
     primer          complement(8599..8622)
                     /note="FRT3"
     primer          12793..12811
                     /note="seq primer NF"
     misc_feature    12853..12860
                     /note="RE sites"
     misc_feature    14653..14686
                     /note="loxP"
     misc_feature    complement(12861..12892)
                     /note="Rox"
     misc_feature    complement(14613..14644)
                     /note="Rox"
     misc_feature    12893..12914
                     /note="polylinker"
     CDS             13473..14069
                     /note="puromycin N-acetyl transferase"
     misc_feature    13588..13593
                     /note="Mutated RsrII site"
     intron          9135..9509
                     /note="mouse En2 intron"
     misc_feature    9507..9509
                     /note="Splice Acceptor"
     exon            9510..9556
                     /note="mouse En2 exon"
     primer          9410..9431
                     /note="seq primer FCHK2"
     misc_feature    9557..9679
                     /note="1Dleader"
     misc_feature    9680..9731
                     /note="F2A"
     misc_feature    9827..10543
                     /note="EGFP"
     misc_feature    9737..9826
                     /note="3X NLS"
     misc_feature    12609..12847
                     /note="SV40 polyadenylation site"
     misc_feature    11642..12586
                     /note="ERT2"
     promoter        12925..13436
                     /note="PGK promoter"
     misc_feature    14180..14612
                     /note="PGK pA"
     primer          12667..12690
                     /note="LRPCR RAF5"
     misc_feature    10553..10606
                     /note="T2A"
     misc_feature    10603..10603
                     /note="A to G to kill RsRII site"
     misc_feature    10607..11632
                     /note="Cre"
     misc_feature    9525..9557
                     /note="FCHK lf2a_lacz_gt2"
     misc_feature    9525..9540
                     /note="FCHK QC prefix region"
     misc_feature    9541..9551
                     /note="FCHK QC critical region"
     misc_feature    9552..9557
                     /note="FCHK QC suffix region"
     misc_feature    14709..14729
                     /note="B2 Gateway"
     misc_feature    8523..14759
                     /note="Synthetic Cassette"
     exon            15162..16966
                     /db_xref="ENSEMBL:ENSMUSE00000455888"
                     /type="targeted"
                     /note="target exon 1 ENSMUSE00000455888"
     misc_feature    14760..17159
                     /note="Critical Region"
     misc_feature    17183..17216
                     /note="Downstream LoxP"
     misc_feature    17160..17239
                     /note="synthetic loxP region"
     misc_feature    17240..22332
                     /note="3 arm"
BASE COUNT     5868 a   5377 c   5257 g   5829 t     1 others
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggattatat gataccgtgt acttgtcatt tttgcgatga agatatttaa
     4321 aacagcctct taacaatttt caaggatgaa aactgtttct cctgtagtca tcccattgta
     4381 gagtgcattg cttgaactgc ccgcgcccca atgggcattg tgaatcccta aacctgaatc
     4441 accacacttc cctccctatc tccaaccact gcctactccc gagactttta taggccccat
     4501 attgtttaga tccacatttg agtgcaactg tgaggccttt gccttttggt gcccagctta
     4561 tttcactgaa cataacgtcc tccggctcat ctatgtttcc aaatgcaggc ctgtcttatt
     4621 ctttaaggca aaatagtatt ctatcgtaca tatgtgtgtg ctgtaagtat gtatgtacat
     4681 gtgcctatga acatatgtgt gtatgcagct taattagtta cctattcatg acacttagtt
     4741 ccttctcatc tctggctttg ccagggactg tttgacttat gtcttgttgc catgataagc
     4801 agtcaacagg aacagccttg ttcattcagc tctctccaca ccctcacact tccgtggcat
     4861 aagttcctta gcgtagagca ggggattctg ggtatccctg atatcctgct aagcagcctg
     4921 aggcagagca tgctctgccc agcatggccc tttcctcatg atcccactga aaatagacaa
     4981 ctgatatttt tcaatcattt cctgttggcc gggcaaacac tgggggtgta tggttctaat
     5041 tggcattttc ttcattatta taagtacaaa taattctctt ttttatgtag aaaaacattt
     5101 ttgacagaat tattgttttc tgagaacagc tttacacata gtcaaaatcc acttcaaaag
     5161 aagaaattgg agtggaacag atgccgagcg tgttgtaatt taagcaaatt acccacttaa
     5221 caacaataac aagcccactt agaaattatt gctcaaaatt cttgcttact aaaaatcaag
     5281 ggcaaaatat ggcttatttt aaaattgctt cggagagttt ctcttttggg aagaaaatgc
     5341 caacactaca ctgaagcgat ccccaagcat ccataggata ttggcattag agtctgccta
     5401 tcaaagggga ggggtgtaga tggtggggca aatgtataca gtaattttac tgagaaacct
     5461 gagccagaaa accaagttca gcaacgtgca ttggagaggt agggacagga gggtctggga
     5521 cttttggggc agtcagtgga gctaataggt gagatctaga gatctagttt tagtgagatg
     5581 cccttttcaa aatgaataaa aagtggagaa gcaattgagg aagaaagcca tgttaacctc
     5641 tgccctctat gtatatgttt tgaagcatgg gcacgtgaga gagtacatag acacacacac
     5701 atacaaacac acgaggtatg tgggtcaggt atggtggtgc atgcctgtaa tcccagaaat
     5761 caggagactg aggcaggagg attctaagtt gaaaaccagc ctgtgttaaa gagaagaagg
     5821 actcttgaca aacagaagca ggggagagag taggattaca tttttaaaga cttcatgaaa
     5881 cttgacaata atatattttt ttatatcaat tggaaagttg tcatgagggt ttagatgcac
     5941 aggaaaccca tggataatct tgacagtttc atctgtaaat ctgaaattct acattcgaaa
     6001 ttttctctaa agaagctgca ccttggcccc atctcaggct ttcttcctct catcttgaaa
     6061 gcatcaccca ggacccactg agatgagaaa actcactgct ttctgcctga gcacattgat
     6121 cacagcagac tctcaacagc ctgttacttc ctgccacgac tagccctgcc cagcactttc
     6181 ttctctggct tatgcaaacc aaggataatt caggcaaatt caagcagtgt ttttttgttt
     6241 atgtcttggt cacatcttgg ctgttttcca gccttccttt gatgtctggg ttacttaagg
     6301 ggtcctttga aaatggtgtt cagttaagta aataaaaagt gaaaccgtgt gtttggggat
     6361 gaagaattgt gctccccaaa gattccactg tgccaaggct ggaaggaggg gtctcctttg
     6421 atccaaaata attaaattgg gctaactggc tagagctctc ttgcctgaca aaaatggcat
     6481 tatttaccat ttgctttcct taaattcaat ttcattttct ttccttcctt tccccatttc
     6541 ttccccatac ctaactccat gctatctggt cacactgatg tatactctgg gttccccaca
     6601 taagccatgt gatatcagcc atccttgtct gtggccacgc tgttctctgt gtgacacagg
     6661 cagtcaacct acggctggac ctattccaca ctgtgcttct cttggatttc actgtgtttt
     6721 tgtttcaaaa ttgagccaac attaaaaaaa aattccttga agttctggag ttcagtcttc
     6781 atttggaaaa tggatgtgct aacactttct gccttgttag ggcatagcca gagctgcccc
     6841 aatactgcaa ctgccaggag attgattgat cttttcttgg tctttgttga ggaaacaaac
     6901 ttggtttctg tcagttgtgt gtctttgttc agattttgag gttttggcta gaaactacag
     6961 aagataaaag aaaaaaaatg taggcttctt cctctctggg aaaattagct aattggatca
     7021 atagagcact actacttgat aaataaaata agttaagcaa atacttaact atgagaacta
     7081 ggctggtatg ggggcttata tctgtaatcc tggctctagg gaagatgctg caagaggttc
     7141 gccacaagtt tgaggcgagc ttgcactaca tagtgggttc cagcctgtct gagacacagg
     7201 ataagatgct ttctcaaaaa tagatgatag atagagaggt aggtaggtag gtaggtaggt
     7261 aggtaggtag atagatagat agatagatag atagatagat agatagatag gagatagatg
     7321 gttcagtggg taatggcatt tgcttttcag tctgatgttt gcagattgac cgccaagaac
     7381 cacatggtga actgagagaa ctatgcaagt tgccctctga catccatatg cacacagtga
     7441 cactcatacc caaacatata cacacagaaa ataaatacat ttaatttctg aaaatatttt
     7501 tttttaaaaa attaaaactc ccacaagaga gttgcagtgg taggaagaag aaatgagaga
     7561 gacacagata ggtaaacctg ggcaggcttg atctgagggt gctgcctgct gggctggagt
     7621 gtagctccat ggcagaacac ttggcaggca cgcatgaggc cctgtgttct gcccttaaca
     7681 ctgcaaatac aaaactgaag gtgtgctgcc tcttaccatc atttaaaacc atcgcacccc
     7741 tcagagtctc gctgtactct agggctgacg agtggttctc agccaaggct tacagggagg
     7801 agacccaagt ctgagtcatc aaatgacaca actctggttt tgcaacatta gcccagggaa
     7861 cctgtgaatc acctgaagat cgtgtcaaga ttctaattca gtaagtctgg gttgggctct
     7921 aaattctgca tttcaataaa accataggta atctattgtt gctgctgact atatgtgtgg
     7981 gtcaaaaaac tacccaaaat aggacagaat ggtacattac aaagtaggac agaatggtac
     8041 attatcgatc tttgtaaggt atggattttt ttctgtttct gtttcagctt tcccaccagc
     8101 agtggtgaga gattttctca attcacccgc ccccctcctt tgcttcagta gggtctgtac
     8161 ttgcatctta aggcctcaga ggaatagagg ctcttttttc tggttgcttt ttcttctgtt
     8221 ttcctcattt gattaaagag agggctgaag tgttggctag aattagctct ctctaagtcc
     8281 tcagagaata ctgctaagtt gtcaggacaa ttgaaagcca attaaacata gaaattattg
     8341 aaatcttata agtgtacact gaacagattt tattaaaatg tagccctcaa tttaccactt
     8401 tatagctagt tcactttttt ttttatatgt gtagtctgaa gttacacatg tctattatat
     8461 tgctacggta gaaataaaat agtagactat tgcacgtatc ttccaatctc tacatccagt
     8521 gaaaggcgca taacgatacc acgatatcaa caagtttgta caaaaaagca ggctggcgcc
     8581 ggaaccgaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttcgaaccc
     8641 tttcccacac caccctccac acttgcccca aacactgcca actatgtagg aggaaggggt
     8701 tgggactaac agaagaaccc gttgtgggga agctgttggg agggtcactt tatgttcttg
     8761 cccaaggtca gttgggtggc ctgcttctga tgaggtggtc ccaaggtctg gggtagaagg
     8821 tgagagggac aggccaccaa ggtcagcccc ccccccctat cccataggag ccaggtccct
     8881 ctcctggaca ggaagactga aggggagatg ccagagactc agtgaagcct ggggtaccct
     8941 attggagtcc ttcaaggaaa caaacttggc ctcaccaggc ctcagccttg gctcctcctg
     9001 ggaactctac tgcccttggg atccccttgt agttgtgggt tacataggaa gggggacggg
     9061 attccccttg actggctagc ctactctttt cttcagtctt ctccatctcc tctcacctgt
     9121 ctctcgaccc tttccctagg atagacttgg aaaaagataa ggggagaaaa caaatgcaaa
     9181 cgaggccaga aagattttgg ctgggcattc cttccgctag cttttattgg gatcccctag
     9241 tttgtgatag gccttttagc tacatctgcc aatccatctc attttcacac acacacacac
     9301 cactttcctt ctggtcagtg ggcacatgtc cagcctcaag tttatatcac cacccccaat
     9361 gcccaacact tgtatggcct tgggcgggtc atcccccccc ccacccccag tatctgcaac
     9421 ctcaagctag cttgggtgcg ttggttgtgg ataagtagct agactccagc aaccagtaac
     9481 ctctgccctt tctcctccat gacaaccagg ctcccaggtc ccgaaaacca aagaagaaga
     9541 acgcagatcg cagatctcta gagtcaccga gttgctttac cggatgaaga gggccgaaac
     9601 atactgtcca aggcccttgc tggcaatcca cccaactgaa gccagacaca aacagaaaat
     9661 tgtggcaccg gtgaaacaga ctttgaattt tgaccttctc aagttggcgg gagacgtcga
     9721 gtccaacccc ggaccggtgc atgtggaccc caagaaaaag cggaaggtgg accctaaaaa
     9781 gaaacgcaaa gtggatccta agaaaaaaag aaaggtgcac gtgtccatgg tgtccaaggg
     9841 cgaggaactg ttcaccggcg tggtgcccat cctggtggaa ctggatggcg acgtgaacgg
     9901 ccacaagttc agcgtgtccg gcgagggcga aggcgacgcc acatacggaa agctgaccct
     9961 gaagttcatc tgcaccaccg gcaagctgcc cgtgccttgg cctaccctcg tgaccacact
    10021 gacctacggc gtgcagtgct tcagcagata ccccgaccat atgaagcagc acgacttctt
    10081 caagagcgcc atgcccgagg gctacgtgca ggaaagaacc atcttcttta aggacgacgg
    10141 caactacaag accagggccg aagtgaagtt cgagggcgac accctcgtga acagaatcga
    10201 gctgaagggc atcgacttca aagaggacgg caacatcctg ggccacaagc tggagtacaa
    10261 ctacaacagc cacaacgtgt acatcatggc cgacaagcag aaaaacggca tcaaagtgaa
    10321 cttcaagatc cggcacaaca tcgaggacgg ctccgtgcag ctggccgacc actaccagca
    10381 gaacacccct atcggcgacg gccctgtgct gctgcctgac aaccactacc tgagcaccca
    10441 gagcgccctg agcaaggacc ccaacgagaa gagggaccac atggtgctgc tggaattcgt
    10501 gaccgccgct ggcatcaccc tgggcatgga cgagctgtac aagcctgcag gcgagggcag
    10561 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc gggccgtcca atttactgac
    10621 cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa
    10681 cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct
    10741 gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc
    10801 agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa
    10861 aactatccag caacatttgg gccagctaaa catgcttcat cgtcggtctg ggctgccacg
    10921 accaagtgac agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga
    10981 tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg
    11041 ttcactcatg gaaaatagcg accgctgcca ggatataagg aatctggcat ttctggggat
    11101 tgcttataac accctgttac gtatagccga aattgccagg atcagggtta aagatatctc
    11161 acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac
    11221 cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc
    11281 cgtctctggt gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg
    11341 tgttgccgcg ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga
    11401 agcaactcat cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg
    11461 gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat
    11521 accggagatc atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg
    11581 taacctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg atctcgagcc
    11641 atctgctgga gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc
    11701 taagaagaac agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga
    11761 tgctgagccc cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc
    11821 gatgatgggc ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc
    11881 gaagagggtg ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg
    11941 tgcctggcta gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa
    12001 gctactgttt gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat
    12061 ggtggagatc ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca
    12121 gggagaggag tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt
    12181 tctgtccagc accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa
    12241 gatcacagac actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca
    12301 ccagcggctg gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg
    12361 catggagcat ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct
    12421 ggaggcggcg gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga
    12481 ggagacggac caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa
    12541 gtattacatc acgggggagg cagagggttt ccctgccaca gcttgatgaa gatctgagct
    12601 cttaattaat gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa
    12661 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
    12721 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    12781 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    12841 atgtctggat ccgtttaaac taactttaaa taattggcat tatttaaagt tacatatggc
    12901 ggccgcccta aggctcgacc tcgaaattct accgggtagg ggaggcgctt ttcccaaggc
    12961 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
    13021 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
    13081 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
    13141 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
    13201 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
    13261 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
    13321 ggctcagggg cggggcgggc gcccgaaggt cttccggagg cccggcattc tgcacgcttc
    13381 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
    13441 tccatctaga tctcgagcag ctgaagctta ccatgaccga gtacaagccc acggtgcgcc
    13501 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
    13561 accccgccac gcgccacacc gtcgatccag atcgccacat cgagcgggtc accgagctgc
    13621 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
    13681 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
    13741 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
    13801 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
    13861 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
    13921 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
    13981 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
    14041 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
    14101 ccgaaaggag cgcacgaccc catgcatcga tgatatcaga tccccgggat gcagaaattg
    14161 atgatctatt aaacaataaa gatgtccact aaaatggaag tttttcctgt catactttgt
    14221 taagaagggt gagaacagag tacctacatt ttgaatggaa ggattggagc tacgggggtg
    14281 ggggtggggt gggattagat aaatgcntgc tctttactga aggctcttta ctattgcttt
    14341 atgataatgt ttcatagttg gatatcataa tttaaacaag caaaaccaaa ttaagggcca
    14401 gctcattcct cccactcatg atctatagat ctatagatct ctcgtgggat cattgttttt
    14461 ctcttgattc ccactttgtg gttctaagta ctgtggtttc caaatgtgtc agtttcatag
    14521 cctgaagaac gagatcagca gcctctgttc cacatacact tcattctcag tattgttttg
    14581 ccaagttcta attccatcag aagctggtcg agtaacttta aataattggc attatttaaa
    14641 gttaggcgcg ccataacttc gtatagcata cattatacga agttatgtcg agatatttaa
    14701 atattagacc cagctttctt gtacaaagtg gttgatatct ctatagtcgc agtaggcggt
    14761 tttattttcc ccatgaatga gcccattgtt aaacaattac cagtataaca ttagatgata
    14821 ttcttggctt tggggaaatt tctaattaag ctcaattaga agtagtcata ttccctttct
    14881 ctgtcatatg acccaactct gaccaatgtg aaatgcaagt gacagctaga aaggtttggg
    14941 ggaagctttt cttctaaaag ggaggcatag gggagggatg gctctttctc tttctgccca
    15001 tctggtatct ggctgtgatg cagccaaact tggcagccat catatgtcaa cgtcaaggca
    15061 gggtaaacat gcattttgtt ggttgttttt gtttttattt gtagacctta tagtcagtga
    15121 aaaaacaatt taacatacat ctttcatatt ttcttctcta gtggagaccc tgataagtgt
    15181 gacatctggg ggaacactcc tctacattat gcagcctcca atggccacac tcattgcatc
    15241 tcatttctag tcaattttgg tgccaacatc tttgccctgg ataatgactt gaagtctcca
    15301 ctggatgctg ctgctagtag ggagcagaaa gaatgtgttg ctctcctgga caaggctgcc
    15361 actgtgcaga acaccatgaa ccccaaaaga gtcaccagac tgaaggaaca ggctctgaag
    15421 aatgccagga agcagatgaa agagtgcgaa cggcttcagg agaggcacca aaacaagatg
    15481 gcgcgcacct atagcaagga ggactcaggg acgatttctt cttcccatag taccttgtct
    15541 aggtcgtttc tttcaactac ttcagctggt agatttggat cactgtccaa aggcattaaa
    15601 gataccttca agataaaatc taagaagaac aaagatacaa cagaacagct agagaaggac
    15661 ggaagaagtg ggcagaggcc tgtgatggaa gtgttcagag aggaggaaga agactcattt
    15721 ccaaaggact tcaaagagaa gctccatttc tcagtagaag aagatgatga tgtacaacat
    15781 gagtctattc tcaaccgtcc gggtctagga agtattgttt ttagtagaaa cagagtactg
    15841 gactttgagg acatctctga cagcaagagg gagttgggtt ttaaaatgcc cagtgagcta
    15901 ttccaaagac aaggagcagc aggaacagtt gaagaagaag aagaggagga ggaggaggaa
    15961 gaggaggaga agagagaggc aaacggcact gcaggtgatc ttccttggga tgaagaagaa
    16021 gtagaatggg aggaagatgc agttgatgct actccactgg aagtgttctt gcagtctcag
    16081 cacctggagg aattccttcc catttttatg agagaacaga ttgatctaga ggctctgctg
    16141 ctttgctctg atgaggacct tcagaatatc cacatgcagc tgggccccag gaagaaagtc
    16201 cttagtgcca tagacaaaag gaagcaggtg ctccaacagc ctgggcagtt ggttgacacc
    16261 agcctctaat ggaggacatt gattaacagg ctagccagag cctggggttc atgctgagtt
    16321 ctcctggaac caccccagga tgaaccctgt gcttggtctc tgtctaatga caaaccactg
    16381 ggtgtaggtg ttagggtttt agagaggaca ctccaaatca gaggctcata ctctgtatcc
    16441 aagtaaacca tcaatgagaa aagaccttgg gagaccttga aatgggaaaa tggtgtttct
    16501 cttccaggct ttgaattctg tgtagaccaa aggctctcac tagtctgaag cccaaagccc
    16561 aacttaatgg atcatttaac agaagcttgc taactgtggg gttgggttga cagtattcct
    16621 gtctgagaat caggacgtgt agcaggtagg gggatggcag gcagaaaatg tggactgaat
    16681 ttctaaagtt catgtatgga agccctaacc cctatgtgaa tggttttgga gataggactt
    16741 taaagatgtg attaaggttg aatgaggtta taagggcagc tcgtaatcca tatgacttaa
    16801 aagacaagga agagtgggaa gggttggttc acactcaaga aaggaggaca gcaggaaggc
    16861 agctgccaac cagccaagga gagaggcact gagaattcga acctggaaac agtttgggct
    16921 ttcagcctac cgagatgcga aaaataaatg tctgttgttt gggccactct gtctgcagtg
    16981 ttcggttatg gaggcctagg ctgacaagca tggtgtgtaa acaagaggat agtgaagaga
    17041 gcaaggagga ttcagaaaaa caatgcctgt tccatctcac aaggtctcta agcgttaagt
    17101 gtcttctgag gtgcaaaaca ggagggaaaa ggtatagttt taggaaggtt gatggtgaag
    17161 agatggcgca acgcaattaa tgataacttc gtatagcata cattatacga agttatggtc
    17221 tgagctcgcc atcagttcac aagcgcttgt tatatagtct agatcttaga tgtgacccaa
    17281 agacccatat gctaatgcct tggtcatctg cctgtggagc tactgggagg aggaggagga
    17341 ggaggaggag gacgacgacg acgacgacga caacgacaac gaggacgagg aggacgtcta
    17401 tccaccagga agagcttatt agaagatgtt aggtcatcaa agcatgccct tgaagaggtt
    17461 attggaattc caaatgcccc tttctttgct ttccaggcaa cttgaacagg cttcctctgc
    17521 cacatgcttc taatgaatca cctcaccaca ggtcccaaag cactaagacc atggtcatgg
    17581 agtgagtgac aagttaaaac aaacctatct ttcttaatta tgaacatatg ttgattcccc
    17641 ttagataggc accgatgaca gaacaaagga acaattctat ccaagttgag ctcaaaacca
    17701 tgaaggtttt ggagttactt aacaggagta tgggtaactg tattagcaca aatggtgcct
    17761 cctgggggct ccctgcctaa gctgctcttt gctgctttca caatgctagg agtggggctt
    17821 gtgaatctct taacttgtat atgcttcatg agcctggcga attctctata gcttctggag
    17881 tcttctgggt ttccttcctc tcaccaatag gcaatgtttc aattcagacg aaagagtgac
    17941 ataatagttt catacacaca cacacacaca cacacacaca cacacacata tgtaattgtg
    18001 tataatagtc acacacacat attccattat cctctattgt acctttctct ccatctctat
    18061 aaactttttc cttctcacag atcctttctg cttttctctc tctctctctc tctctctctc
    18121 tctctctctc tctctctcac acacacacac acacacacac acacacacac acaaactcac
    18181 aaacttaaca gtcttatgac tcctgaaaag tcttaatttc attctttaca gttgaataaa
    18241 attcagttgt gtaatgcact acattttctt ttcccattca ctggttgatg gacttcagac
    18301 tggtcctatt tcctggtgac tgtgaacagt acaacaataa atatggatgt gcaggtctgt
    18361 gtggtatatt gactgggcta tctgggccac atgatagtta tgttttcagt ttttgaaggg
    18421 ctgtcaagct ggattccata gtttactttt ccaccagcaa tgtatgaagt ccctctttat
    18481 aaaatcttgc cagcattgtt ttttaatgct gacagttttt aaatggcaat ctcaattgaa
    18541 accaccaata gctaagtttt tttttaattt tgtagccaga ctactaccaa gtagaattta
    18601 tcattgactg tgtatataat attataacca ttattttagc ccagctgatt tctcacgtac
    18661 atcttaagtt actttaagag gctcattcag ggtacagatg ctcacatgct caaactcact
    18721 cattcctctc cagtgaaggg attcaagagc atatgggttc ttggatatgt tactagggga
    18781 ttcttgttta caaatactgt gtttggttgt ggaataaact gaagttgtgc ttgtcctcaa
    18841 tactgtgcat agaagcagtt gtcataggaa ggcactcaca ggttctgtga cccaggcctg
    18901 cctctctcac acaccctcac tgtgaaggat cctctctaat tctctagaaa ctgtttgaga
    18961 tactgtcccc aacttacata gtgaggggag gctggatggg gaggcatgac cctaaatgtg
    19021 gggactcctg gtgcttttgt aagatccatt ggtcatgaga tatgttctcc ttgtagagcc
    19081 ttttgatatc aattcgctta gactcaagtg acccacacct gagtagtttc tattactcat
    19141 gaccaaagga gccccttgat ctgtacttct tcaactattt aaaatacaca caggaagcta
    19201 cattacgtgt gattggatgt acatgaaagt tcttgaagag gcaaatctgt agcagtagaa
    19261 actgctggga agaaggtggt gacactgttg ggaatcaggt ttctgcaggg catgggatca
    19321 ttctggactt ggttgtataa aagtcaccga aacccactga attgtaatgc cttaaagtcg
    19381 tgagctgtgc cagggatgta gcttaaagaa agtgtgtttg cctagcatgt gtgaagctct
    19441 gggtttgatc tggtgtgtag catggctaca cacacacaca cacacagagg caggggggat
    19501 ggagggagaa taaaaatggt tttatggtga cttctactca attaaaatag agaaattttt
    19561 aatccaatcc tctagggatt gagtgggcat ttgatacttt tatttctttc ccatagaaag
    19621 ttgtcaaagg acacacagaa gaagtggaaa tgatttattt cagagagata caagactagt
    19681 aagatcaagg gtagataatg tggccatcac cccttaacag aagaaatggt tacctggttt
    19741 agctatccaa ccacaaacat gaataggggt gtcagtactt atgaatgaca atcactacca
    19801 ttcactgggg actggacctc ctttgatttt gacacttgtg aaacagtgtt gagctgtgat
    19861 ggccaactca ggcacggttc cttcaactca cttgccagat gaccaagaat catatactaa
    19921 tttggctgca accaggtctt ccactgccat ccctggaatg gatagaaatt tgcttcactt
    19981 tttttgtcag aacaactttg ccctcaaatg actctggcta tcttctgatg ccctgtttgc
    20041 atgctaaggt ccatagtgat aaccataaat gcttgcccag actcagcata gaaagggctg
    20101 gtcaacaaga ggagtatgtt actaagtaag acatttccac attttctaac ctctaaggtc
    20161 gagttcagag gtcagcaaca tttcacttga ggctcagtga atagtttaag ctccatacat
    20221 catattgtct ctatcacatg gcccagttct gcctttatag taggaaagta gctacagtca
    20281 cagggaaagc aaatgagact gtattttggt aagatcttac tcatgaacac tacacattga
    20341 attcatcact ctctccaccc cctccctgag tgcagaggcc aatgtttgta ttagttatct
    20401 tccttttcca cggctcaatt gttcatttta cttgggtttt ttgtttgttt tttgttttgc
    20461 tttgttctga gtcagggtct tgtgctgagc ttgctggttt ggctacactt aaccagtgag
    20521 ccccagggat ctccatgttt ccacctccat aacaccagaa ttacaggccc agccatgtgc
    20581 tgccacacct ggtactttat acaggtcctg ggggatccaa attcagatct tcctgcttat
    20641 gtgacaagca gtttcccaac taagctgcct ccctgcccag tctttcccat cattcttgag
    20701 ggtcccaaga gcgatttttt atttgtccat cccaatcatt taaaactatg gacactcttt
    20761 tcagctcttg gctcatacaa ctgcaggtag ttggcaggat tggacccatt ggcctagatt
    20821 ttgctgagcc tggaccttca caaatcctgt ctctttagct ttccccattt gtctttgaaa
    20881 tgttgaatat ccctgatcca aaaatttgta atctgaaaca ttacatagtc tgaaactctt
    20941 taagataatg ttgaaattca ttttttttcc agtttgggat cagtttttca tactgggcag
    21001 agggcagggg gcttggtcaa tcaacaatct acaaacattc tctacagttc aaaaaggacc
    21061 caacacttat gagtacaagt atttcagata agatttgttc aatctgtaat tttaagacac
    21121 aggccttagt ctttgtaccc aatattctat gagaggctca acatatatca gctagaagag
    21181 agagtgtgtc agtaaaccag tcacctgagg tgggagcaca aaatttgtca aataagtgga
    21241 ttcctacagt atttgtctcc ttaataaggc cagctaacct tccagactcg actggattga
    21301 tgttgttgga ccaggtacta gatgacattt actacctttg ttggtctttt agtgcacaca
    21361 caaaaaaatt aaaacataaa caacaacaac aaaaaaaact taactacctg tcaaggcctg
    21421 gatctaactg atggtaaaga actgggtaac cagagcacac tttctattac atgctaagac
    21481 catacatgtt aatatcacac atgcctacaa tatgatcaaa tccattccct ctttccttgc
    21541 ttccaattcc ttcccatctc cacatcctca ttctctgcca actttctcct cctcctcctc
    21601 ctcctcctcc tcctcctcct ccttttcctc ctcttcctcc tcctcctttt cctcctcttc
    21661 ctcctcctcc tccacctttt gaaaataagt ctccttggtg atacttgtac gtgcatggca
    21721 ttctctcaag gtctcaaggg ccacattcct gaagaaaact gacttttcaa gccccaatct
    21781 gccatcagtt acctgcagct tctaagctag ggggtaggat gtaataagcg ctattccccc
    21841 attcacatcc atctttcggt tggcttgctc ttgtgcaggc cttgtgcatg gggtccaggc
    21901 actgggattt catgcgtgca gaaaccctct cacagccagc aaatgttgtt tcactgtagt
    21961 ggccacactg cccgccagaa cactccttgt ttggtttttc atttgttttt gctttgttgt
    22021 attttgagac agggtctctc tatttaatcc tggctgcact ggaacttgct atgaagatca
    22081 gactagcctt gaactcacag agagctgcct ctgccatttt agtgttggga ttaaaggtgt
    22141 gtgccaccat atccagccat ctggagtact tttaaaactg tggcagatat taaaaatgtt
    22201 tctgctgctc aatatacaga agtatggaaa atcctggtaa tatccactta gtaaccatta
    22261 ataatgaaaa aacattaaat ggaatagttg attaccactt tggttcccat ctacatctgc
    22321 ttctttaagc ac
//