LOCUS       78164#pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21043 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 78164
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000026500
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            4478..4600
                     /db_xref="ENSEMBL:ENSMUSE00000789563"
                     /rank="1"
                     /note="ENSMUSE00000789563"
     exon            7135..7219
                     /db_xref="ENSEMBL:ENSMUSE00000150820"
                     /rank="2"
                     /note="ENSMUSE00000150820"
     exon            8466..8540
                     /db_xref="ENSEMBL:ENSMUSE00000150813"
                     /rank="3"
                     /note="ENSMUSE00000150813"
     misc_feature    4275..9843
                     /note="5 arm"
     misc_feature    9871..9895
                     /note="B1 Gateway"
     misc_feature    9908..9955
                     /note="Frt"
     misc_feature    9956..10455
                     /note="mouse En2 intron"
     primer          complement(10024..10045)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9920..9943)
                     /note="FRT3"
     primer          14107..14125
                     /note="seq primer NF"
     misc_feature    14167..14174
                     /note="RE sites"
     misc_feature    15967..16000
                     /note="loxP"
     misc_feature    complement(14175..14206)
                     /note="Rox"
     misc_feature    complement(15927..15958)
                     /note="Rox"
     misc_feature    14207..14228
                     /note="polylinker"
     CDS             14787..15383
                     /note="puromycin N-acetyl transferase"
     misc_feature    14902..14907
                     /note="Mutated RsrII site"
     intron          10456..10830
                     /note="mouse En2 intron"
     misc_feature    10828..10830
                     /note="Splice Acceptor"
     exon            10831..10870
                     /note="mouse En2 exon"
     primer          10731..10752
                     /note="seq primer FCHK2"
     misc_feature    10871..10993
                     /note="1Dleader"
     misc_feature    10994..11045
                     /note="F2A"
     misc_feature    11141..11857
                     /note="EGFP"
     misc_feature    11051..11140
                     /note="3X NLS"
     misc_feature    13923..14161
                     /note="SV40 polyadenylation site"
     misc_feature    12956..13900
                     /note="ERT2"
     promoter        14239..14750
                     /note="PGK promoter"
     misc_feature    15494..15926
                     /note="PGK pA"
     primer          13981..14004
                     /note="LRPCR RAF5"
     misc_feature    11867..11920
                     /note="T2A"
     misc_feature    11917..11917
                     /note=" A to G to kill RsRII site"
     misc_feature    11921..12946
                     /note="Cre"
     misc_feature    10846..10871
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    10846..10861
                     /note="FCHK QC prefix region"
     misc_feature    10862..10865
                     /note="FCHK QC critical region"
     misc_feature    10866..10871
                     /note="FCHK QC suffix region"
     misc_feature    16023..16043
                     /note="B2 Gateway"
     misc_feature    9844..16073
                     /note="Synthetic Cassette"
     exon            16446..16642
                     /db_xref="ENSEMBL:ENSMUSE00000150809"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000150809"
     misc_feature    16074..16829
                     /note="Critical Region"
     misc_feature    16853..16886
                     /note="Downstream LoxP"
     misc_feature    16830..16909
                     /note="synthetic loxP region"
     exon            17485..17593
                     /db_xref="ENSEMBL:ENSMUSE00000150817"
                     /rank="5"
                     /note="ENSMUSE00000150817"
     exon            17684..17794
                     /db_xref="ENSEMBL:ENSMUSE00000150793"
                     /rank="6"
                     /note="ENSMUSE00000150793"
     exon            18076..18278
                     /db_xref="ENSEMBL:ENSMUSE00000150802"
                     /rank="7"
                     /note="ENSMUSE00000150802"
     exon            18707..18785
                     /db_xref="ENSEMBL:ENSMUSE00000150801"
                     /rank="8"
                     /note="ENSMUSE00000150801"
     exon            18905..19003
                     /db_xref="ENSEMBL:ENSMUSE00000150795"
                     /rank="9"
                     /note="ENSMUSE00000150795"
     exon            19673..19826
                     /db_xref="ENSEMBL:ENSMUSE00000150799"
                     /rank="10"
                     /note="ENSMUSE00000150799"
     exon            19921..20021
                     /db_xref="ENSEMBL:ENSMUSE00000150803"
                     /rank="11"
                     /note="ENSMUSE00000150803"
     exon            20466..20603
                     /db_xref="ENSEMBL:ENSMUSE00000150797"
                     /rank="12"
                     /note="ENSMUSE00000150797"
     exon            20845..21003
                     /db_xref="ENSEMBL:ENSMUSE00000150811"
                     /rank="13"
                     /note="ENSMUSE00000150811"
     misc_feature    16910..21043
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaagtgca cttttaaaaa aatcaataga ccagggtgtg gagctggagt
     4321 taaaagaagc ctttagaagt ctgctcttct tgttttcgct gttttgcata ggagcagata
     4381 aagctttcct ctctggtttg aataagtcaa gcccagggct aggttggctg tgattggcca
     4441 gagctaggag aatgtggtta tgatgcaaac acaagcaaat ataaccctgt gtctatgctg
     4501 tgcggccatt gctgaaacag gcttcaggac gggctgcttt gggagacctc atcagtactt
     4561 cacctgctgc tttgccggac ttcggaggac agagggtcag gtaggtctac tctgtgtgtc
     4621 cttgtcagcc aaatgctgag tgtggatgag gccaaaggtg aagaagggac aagagtcata
     4681 ccttccctgg cttttgtctt tggggatgca gctttggaaa gatggagctt tgcaaacttc
     4741 acactgagag aacctgtaga tcccactggc taggttgggc acgttcttga ggctcaggga
     4801 tttggggtaa caagacccct ctcatctgtt ccactgtggg gcaagagaag tttaaacccc
     4861 agtaggttac aagctacctg gaatccccag accatacccc gtgcctgtga tctttgtagc
     4921 tctccgtacc ccaaagtgct ggtggttacg gcttgggaag acaaacagac ccagctttgg
     4981 gggactttga tgtggataga tggaactggg ctttctgggt gtggcatttc ctgaatcttg
     5041 ggtctctaat cagctcctgg attctgaagt tccatggggg acatgggact gcactggggg
     5101 ctcggattca ccgtgctaag gttgctggtt aggctttacc gataaccagg actgtgtcag
     5161 ctctggttcc ctttcctggg tgggtcacgc attagactta aaagtgagag aactcggcct
     5221 gaaggaaagc tgactatttc ctggtggttc tgagctgtgc agggtggggc agcacaagtt
     5281 atcaaattaa atactcaaac attggcagtt ccgcaccacg gctctgcttc agtctactgc
     5341 ggcatcacac aaaggggcct ggctctttgg tggtaatggt gcatcctctc caaacctggt
     5401 tagatccatt agggctcaag aaaagaatgg tcggtggagt cagacccttt tttttctttt
     5461 aggggtgggg ccttgctggg gatgacagac gcctactatt tgaacaatgc cccatcccca
     5521 gacttaacat agttaacaag ccctgaatat caccaactat caaacatcca cacaatctcc
     5581 ctccccctcc aatcccccgg gacgattttg tcttttaatc tctcgtaagt ccaaccatcc
     5641 catctttgag tcttaggaga agccaaccta gcgatcactt ggcataccgt gagagctcag
     5701 tctccccgag ggtaggactt gagatgagac atgttcgcag acctactggg tcacctcgtg
     5761 tgttaacccc agccaagtgc tgactactgt gaccagcgta agatccccgt ggtcatctta
     5821 ccacttccct ccctggcctt attcagctct atgggtgctg ggaacctcta agctccctga
     5881 gaacaagcct tccagagaag ctggggtctg cgtgggacag gcagggaggc ctgcacccat
     5941 tttccttgct gacacactca gctctttgtc ctaggcacag gcattgtacc ttcccctttc
     6001 ttctgcaaga ccagttcatg tattagtcat gagaaagggt ggttactgtt ctaggatgat
     6061 ttgcaaagtt aaaagtgata gccaagagca ggtgggggtg acaaggtctt gccaggcatc
     6121 tgggaagagg agttcaagaa ttgggaggag gggcttcatt tggaaatact ttttctgtct
     6181 ggatttcctc tctgtgagca actgaactta gttttgggaa ggctacagca tgaaaacatg
     6241 gctaaaacac agacttttaa gggcaggtgt gaaagaaaat aaaagggatg gacctaggtc
     6301 tggtggcaga agcctgtaac gctggcactc aggaggctaa ggcagctaaa gcagaggagt
     6361 ttacgaccag cccgggctac atggatagat cctgtgctga taaaatgtgc caggcagtgg
     6421 tgggtcatgt ctttaattcc agatctcggg aggcagagac agggggatct ccttgagttc
     6481 agggccagcc ttgtctacag agtgagttcc agcatggcca gggtctcagg gaccctttct
     6541 tgaaaaaagt aaaacaaaat gaaaccaaaa aagctcagct ctggggccca tgctagcgtg
     6601 tctatagaca gattatctgc aatctctcta agcctcatct gtaccacagg tgacagggag
     6661 atggccactt cctgaaaatg agccactgtg ggcagtgggt caacatgggc gtgacattgt
     6721 cacactcagg aatatgtgtt gcctttcctg ccacccctcc tcctcgccca tagagagatg
     6781 gcacatactg gcccagcacc tacttctggt gactcacgac cgcctgtcac tccagctcca
     6841 cagaatttaa cacaccaaca tgcatgtatg ttttaaagga aatgtgtcat ccctatggcc
     6901 cagagctctt gaccaaggtg acagcaggtg ggagacaagc ctttcgagga gggaatgccc
     6961 agccaggtca ctatgtgaac ttgggctgcc atggtgaaat ggtgtggtgg tggcatggtg
     7021 atataccgaa tggggatgcg gtgtggtggc tgacgagaca gggaagtgtc ttgggagaag
     7081 caatggctcc ctgttcactg tgagtaggat caccccgact ttgtgatgtt tcagttccag
     7141 gaagacagcc actatgtctc tgagcagtgc cttcagggct gtgagcaacg acccccggat
     7201 catcacatgg agaattgagg tgagccgcgt gggagccaca cccaccctga ccactgggat
     7261 ctgaatcagt ggctatagaa ggtcagcatc agagggcagg cttccccaac cttgaacctc
     7321 ccagtgactc agtgcaggcc cccaagcgga accaaccttg tccttcatcc gggcatctgg
     7381 ttggtgagtc tacactccca ataaacatct cagaaagaca ggccccttaa ccgaagactc
     7441 ttggctgctg gtcagaacat gtctggttcc taccagcttt gggatgggac atgctgttca
     7501 aacgggcact gctcgtctct ttatcctgtg gccttggtct cgagggtggc tctgtgctct
     7561 ggtctggaag cacctaccag atactaagac atttgcacct ctacctagtc agttgttgca
     7621 gcctgaaccc cacaggtaac atttcccttc accctgctca gtttcaaagt gtgagccacg
     7681 agctctcaag gcttgtggag tctacttcca gtgccaggct cctcactcac ttcagaatga
     7741 ataggaaggg ctggccagag ggagatgaat gtgtgcagcc aagcctgctg actaagtgca
     7801 agccctgggg cccacatggt ggaagaaggg aaccaacacc gagtttctct gacatttaca
     7861 cgtaccgtgt agcacacaat aagtgtaagg aagaaaggaa cggacaggca ccaacctgga
     7921 ctcatgaagc gtatttaata ttttcacacc taacatccgg gttttacata gaacatacac
     7981 aaagatggca acgggtttgc ttcacttgaa cagagaaagt atcatttgta tctgaggccg
     8041 tgtgaaccgt cttcctcaga tgagcaaatc agggccccac aacccagact gtgactgtgg
     8101 gaccctcagc ctggagcacc acacgccgcg ggcgttctct atgctgtctg cctgctctcc
     8161 tgcctggaca atccacacag ctccaatgag ggttacttgg cagttacacc caactccttc
     8221 ccccgctgtg ggctttgggg gctcacaagc ctccccaggg aaccgtgaat gagaccaaaa
     8281 ggaggctatg gggaaggaca ccagaacact cagacccaga gtgctcctag aagccagagc
     8341 agtgctcctg tccctgtaaa gatagggaga tggttcctct gctacctgtg cgggtgacag
     8401 gagagggagt ctgtggggaa ggcaggagcg agggacacag ctgcatactc tgctaatcct
     8461 tctagaaaat ggagctggcg ctggtgcccc tgagtgctca cggcaacttc tacgagggag
     8521 actgctacat cgtcctctcg gtgagcgatg tcctccacag ctgtgttctc cctcagctcc
     8581 caggctgtag acagctccca cggtgaaagt ggtggggttt gcattgtctg tgtagactct
     8641 tcctcatatg gttggacaag ggccaccatc aggcctgggt tgtgattgtg tgtgtgtcag
     8701 atggtgggcc tagggccctc agtccaaccc ttctcctggg tgtaagctgt ccacacaccc
     8761 acacacatac catacacaca cagcacacac accccagggt cccagccata cactaaagtg
     8821 ttcagaaggc cagacaaaca tgcaacctga ctttggccac aaagtgctca cggtgcctta
     8881 ggtatgatag tttctattgc tgtgacaaat gccctgagca aaagcatctt gggattcatt
     8941 catcttatgt cgctgaggaa agtcaggcca ggagctcaag gcagaaacct ggaggcagaa
     9001 actgaagcag agcccacaga gcaatgctgc ttactggctt gcttcctgtg gttccccccc
     9061 accctcccag acagggtttc tctgtgtagc cctggctgtc ctggaactca gtgtgtagac
     9121 caggctggcc ttgaactcag aaatcaccct ctgcctctgc ctctctgcct ctctgcctct
     9181 ctgcctctct gcctctctgc ctctctgcct ctctgcctct gcctctttgc ctctctgcct
     9241 ctctctctgc ctctctgcct ctctgcctct ctctctgcct ctctgcctct ctgcctctct
     9301 gcctctctgc ctctctgcct ctgcctctgc ctctgcctct gcctctgcct ctgcctctgc
     9361 ctctgcctct gcctctgcct ctgcctctgc ctctgcctct gcctctgcct ctgcctctgc
     9421 aagtgctggg attaaaggcg tgagccacca ctgcccggct cttgtggctt tttaaaatgt
     9481 acccgaggac cacctgccca tccgtcgtgg atcaggaaaa cgcactacaa acgtaccccc
     9541 gggccatctg atggaggcat tttttttttc ttaatccacg ttcttcctcc cagataactc
     9601 cagcttgtga caagttgaca ggatagacag tataccagtc ataacactca ggaagaccaa
     9661 cttcaaggtc agcctgggct acatattaaa tccttgacta gcctgggcta caggcaaaca
     9721 ttcagtctcc taaaaccaaa gaatggaacc tagtggcaca cacctatgat cccagcactt
     9781 gagagttgga actagggaga ttaggaggtt ggaggcctgc ctcaacaagt tttgactagc
     9841 ttgaaggcgc ataacgatac cacgatatca acaagtttgt acaaaaaagc aggctggcgc
     9901 cggaaccgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcgaacc
     9961 ctttcccaca ccaccctcca cacttgcccc aaacactgcc aactatgtag gaggaagggg
    10021 ttgggactaa cagaagaacc cgttgtgggg aagctgttgg gagggtcact ttatgttctt
    10081 gcccaaggtc agttgggtgg cctgcttctg atgaggtggt cccaaggtct ggggtagaag
    10141 gtgagaggga caggccacca aggtcagccc ccccccccta tcccatagga gccaggtccc
    10201 tctcctggac aggaagactg aaggggagat gccagagact cagtgaagcc tggggtaccc
    10261 tattggagtc cttcaaggaa acaaacttgg cctcaccagg cctcagcctt ggctcctcct
    10321 gggaactcta ctgcccttgg gatccccttg tagttgtggg ttacatagga agggggacgg
    10381 gattcccctt gactggctag cctactcttt tcttcagtct tctccatctc ctctcacctg
    10441 tctctcgacc ctttccctag gatagacttg gaaaaagata aggggagaaa acaaatgcaa
    10501 acgaggccag aaagattttg gctgggcatt ccttccgcta gcttttattg ggatccccta
    10561 gtttgtgata ggccttttag ctacatctgc caatccatct cattttcaca cacacacaca
    10621 ccactttcct tctggtcagt gggcacatgt ccagcctcaa gtttatatca ccacccccaa
    10681 tgcccaacac ttgtatggcc ttgggcgggt catccccccc cccaccccca gtatctgcaa
    10741 cctcaagcta gcttgggtgc gttggttgtg gataagtagc tagactccag caaccagtaa
    10801 cctctgccct ttctcctcca tgacaaccag gctcccaggt cccgaaaacc aaagaagaag
    10861 aacgcagatc tctagagtca ccgagttgct ttaccggatg aagagggccg aaacatactg
    10921 tccaaggccc ttgctggcaa tccacccaac tgaagccaga cacaaacaga aaattgtggc
    10981 accggtgaaa cagactttga attttgacct tctcaagttg gcgggagacg tcgagtccaa
    11041 ccccggaccg gtgcatgtgg accccaagaa aaagcggaag gtggacccta aaaagaaacg
    11101 caaagtggat cctaagaaaa aaagaaaggt gcacgtgtcc atggtgtcca agggcgagga
    11161 actgttcacc ggcgtggtgc ccatcctggt ggaactggat ggcgacgtga acggccacaa
    11221 gttcagcgtg tccggcgagg gcgaaggcga cgccacatac ggaaagctga ccctgaagtt
    11281 catctgcacc accggcaagc tgcccgtgcc ttggcctacc ctcgtgacca cactgaccta
    11341 cggcgtgcag tgcttcagca gataccccga ccatatgaag cagcacgact tcttcaagag
    11401 cgccatgccc gagggctacg tgcaggaaag aaccatcttc tttaaggacg acggcaacta
    11461 caagaccagg gccgaagtga agttcgaggg cgacaccctc gtgaacagaa tcgagctgaa
    11521 gggcatcgac ttcaaagagg acggcaacat cctgggccac aagctggagt acaactacaa
    11581 cagccacaac gtgtacatca tggccgacaa gcagaaaaac ggcatcaaag tgaacttcaa
    11641 gatccggcac aacatcgagg acggctccgt gcagctggcc gaccactacc agcagaacac
    11701 ccctatcggc gacggccctg tgctgctgcc tgacaaccac tacctgagca cccagagcgc
    11761 cctgagcaag gaccccaacg agaagaggga ccacatggtg ctgctggaat tcgtgaccgc
    11821 cgctggcatc accctgggca tggacgagct gtacaagcct gcaggcgagg gcagaggaag
    11881 tcttctaaca tgcggtgacg tggaggagaa tcccgggccg tccaatttac tgaccgtaca
    11941 ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca agaacctgat
    12001 ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc ttctgtccgt
    12061 ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc ccgcagaacc
    12121 tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag taaaaactat
    12181 ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tctgggctgc cacgaccaag
    12241 tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg ttgatgccgg
    12301 tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg ttcgttcact
    12361 catggaaaat agcgaccgct gccaggatat aaggaatctg gcatttctgg ggattgctta
    12421 taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata tctcacgtac
    12481 tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta gcaccgcagg
    12541 tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga tttccgtctc
    12601 tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa atggtgttgc
    12661 cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt ttgaagcaac
    12721 tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg cctggtctgg
    12781 acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt caataccgga
    12841 gatcatgcaa gctggtggct ggaccaatgt aaatattgtc atgaactata tccgtaacct
    12901 ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgatctcg agccatctgc
    12961 tggagacatg agagctgcca acctttggcc aagcccgctc atgatcaaac gctctaagaa
    13021 gaacagcctg gccttgtccc tgacggccga ccagatggtc agtgccttgt tggatgctga
    13081 gccccccata ctctattccg agtatgatcc taccagaccc ttcagtgaag cttcgatgat
    13141 gggcttactg accaacctgg cagacaggga gctggttcac atgatcaact gggcgaagag
    13201 ggtgccaggc tttgtggatt tgaccctcca tgatcaggtc caccttctag aatgtgcctg
    13261 gctagagatc ctgatgattg gtctcgtctg gcgctccatg gagcacccag tgaagctact
    13321 gtttgctcct aacttgctct tggacaggaa ccagggaaaa tgtgtagagg gcatggtgga
    13381 gatcttcgac atgctgctgg ctacatcatc tcggttccgc atgatgaatc tgcagggaga
    13441 ggagtttgtg tgcctcaaat ctattatttt gcttaattct ggagtgtaca catttctgtc
    13501 cagcaccctg aagtctctgg aagagaagga ccatatccac cgagtcctgg acaagatcac
    13561 agacactttg atccacctga tggccaaggc aggcctgacc ctgcagcagc agcaccagcg
    13621 gctggcccag ctcctcctca tcctctccca catcaggcac atgagtaaca aaggcatgga
    13681 gcatctgtac agcatgaagt gcaagaacgt ggtgcccctc tatgacctgc tgctggaggc
    13741 ggcggacgcc caccgcctac atgcgcccac tagccgtgga ggggcatccg tggaggagac
    13801 ggaccaaagc cacttggcca ctgcgggctc tacttcatcg cattccttgc aaaagtatta
    13861 catcacgggg gaggcagagg gtttccctgc cacagcttga tgaagatctg agctcttaat
    13921 taatgatcat aatcagccat atcacatctg tagaggtttt acttgcttta aaaaacctcc
    13981 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
    14041 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    14101 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    14161 ggatccgttt aaactaactt taaataattg gcattattta aagttacata tggcggccgc
    14221 cctaaggctc gacctcgaaa ttctaccggg taggggaggc gcttttccca aggcagtctg
    14281 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
    14341 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
    14401 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
    14461 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
    14521 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
    14581 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
    14641 ggggcggggc gggcgcccga aggtcttccg gaggcccggc attctgcacg cttcaaaagc
    14701 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcatccatc
    14761 tagatctcga gcagctgaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
    14821 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
    14881 ccacgcgcca caccgtcgat ccagatcgcc acatcgagcg ggtcaccgag ctgcaagaac
    14941 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
    15001 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
    15061 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
    15121 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
    15181 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
    15241 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
    15301 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
    15361 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
    15421 ggagcgcacg accccatgca tcgatgatat cagatccccg ggatgcagaa attgatgatc
    15481 tattaaacaa taaagatgtc cactaaaatg gaagtttttc ctgtcatact ttgttaagaa
    15541 gggtgagaac agagtaccta cattttgaat ggaaggattg gagctacggg ggtgggggtg
    15601 gggtgggatt agataaatgc ntgctcttta ctgaaggctc tttactattg ctttatgata
    15661 atgtttcata gttggatatc ataatttaaa caagcaaaac caaattaagg gccagctcat
    15721 tcctcccact catgatctat agatctatag atctctcgtg ggatcattgt ttttctcttg
    15781 attcccactt tgtggttcta agtactgtgg tttccaaatg tgtcagtttc atagcctgaa
    15841 gaacgagatc agcagcctct gttccacata cacttcattc tcagtattgt tttgccaagt
    15901 tctaattcca tcagaagctg gtcgagtaac tttaaataat tggcattatt taaagttagg
    15961 cgcgccataa cttcgtatag catacattat acgaagttat gtcgagatat ttaaatatta
    16021 gacccagctt tcttgtacaa agtggttgat atctctatag tcgcagtagg cggtttaaaa
    16081 ataacctttt gcttgttcta ctggatgaat actatcacgc ggacagtgct gaaaaatgtg
    16141 cagttctgtg agcgcggggg cccttcatac agagcctgaa atccaactgg ggaaaggttc
    16201 cccactttag gaacagcaag tggtcagctt ccccaaatgt gggaaagaaa cggggctatc
    16261 agagcttcag cacgcctgta aacaggcagt gctggagcac agagaaagaa gggctgcatg
    16321 tgtgaagggg ccttgccctg aagccccgta gcacagggca tgctggcgcc ttcccacacc
    16381 agagaagtca gcaggatagc agaggtgagc ctagacctga ccctgcccct ctggctacat
    16441 cccagacccg gagagtgggc agccttctct cccagaacat ccacttttgg atcgggaagg
    16501 actcctccca ggacgagcag agctgcgcgg ccatctatac cacacagctg gatgactacc
    16561 tgggaggcag cccggtgcag caccgtgagg tccagtacca cgagtccgac accttccgtg
    16621 gctacttcaa gcagggcatc atgtgagtag ctacgcacag agcggagaca gatacaacag
    16681 ttcccaggag aggaaactaa agggtcagtg agcctttgaa gtaacctgag agatgcaaag
    16741 caaagcaaag ccatcgccaa atcagagtgg ggtgggacaa agggctgggc aggctgtgag
    16801 gagccatacg aggcggtgag ggacattacg agatggcgca acgcaattaa tgataacttc
    16861 gtatagcata cattatacga agttatggtc tgagctcgcc atcagttcaa tctagaatgt
    16921 tcctgactgc gttagcaatg ggaggaggct gtggtcaacg taggtgtctg tgagtcagtg
    16981 gcaagcctca ccatgaacaa aaacacagac aatctctgtt tctgaaagtc gtgcacacgg
    17041 gtgacaggac acgctttatt agaatgaaag agggacgtga gactgcacag aataagttga
    17101 ggcagggaga gttctgggag gtcacacgta ggcgctgcgg tgcacagaca ccacacatga
    17161 gatccagatg gggctcttgc ctataatccc aatctacggg ttccttctgc ctgctgcact
    17221 actctcgtct gtgggacttc tcataaaatc tcagctcaca tgtcccttca ggaaagcccg
    17281 cctcagcccc tagtcacaga cctatccttg gggcagcagg tgcagtatct gtctactttg
    17341 ctcatttgtt attatcattt gttattataa gcaatgtaaa cagaggcaga tgtctgtatg
    17401 ttactactgt aattcttagt agtcttccca gaacctagcg gtgtagcagg cataggacaa
    17461 ataactagat caatgtcccc tcagctataa aaaggggggt gtggcctctg ggatgaagca
    17521 cgtggagacc aacacctatg atgtgaagag gctgctacac gtaaagggga aaagaaacat
    17581 ccaagccacg gaggtgagtc tcacacgtga gtgtcctggg tgcccggtgc tcccgccctg
    17641 gcacctaaga ctgccccgaa ccgctctgac tgctcacacg caggtggaaa tgagctggga
    17701 cagctttaac cgaggcgatg tcttcctgct ggaccttggg atggtcatca tccagtggaa
    17761 tggcccggag agcaacagtg gggaacgtct caaggtctag ctcctccccc tcctctcctc
    17821 caggcctgtc gtccagctgg gctccccctc ttgggtgcca ccttcctttg ccttctccgc
    17881 ttgctgaccc ccaggagttc aggggggggg ggacttctgc ctaagcttag actctgtctt
    17941 gcaggcacgc caagggcttg tcttgctcct cagcatcaga gtaatgtgcc tgccatcctg
    18001 gtggctcgaa tggaagcctt ggggagggaa agggagccag ctgagctgag agccctcctt
    18061 tggcttctct ggcaggctat gcttctggca aaggacattc gggacaggga gcgagggggc
    18121 cgtgctgaaa ttggggtgat tgagggagac aaggaggcgg ccagcccagg gctgatgaca
    18181 gtcctccagg atacccttgg tcgacgctcc atgataaagc ctgcagtttc tgatgagatc
    18241 atggaccaac aacagaaatc aagtatcatg ttatatcagt gagtgtttta gcttggctgt
    18301 gggctggagg ctgggtgtgg ggaggtgtcc tctagaacag caggcactca gctcgggtag
    18361 agacgtcggc ctttgctctt ggttcgacct ttctaaaggt atctcctaca aggagctcag
    18421 agacagccca tctcagagag gcagctatcc tcccttcttg tttaagtctc ctggcctgcg
    18481 ggtagaacct ttctggtgta tccatgccta ggggtgtgga tgggatagtt gtagagcgag
    18541 atccacagtc tccttcagag aacagagtat atctccctct ctgtattcaa gctgtgtcta
    18601 tctccaaaag tccactctgt ctagaaatgc cacactgaaa catcacttta gctatgggat
    18661 gcttcataaa gagaaggcgc caggctaaat ctctctcctt caccagtgtc tccgacacag
    18721 ctgggcagct gtcagtcaca gaggtggcaa caaggcccct ggtccaggac ctactgaacc
    18781 acgatgtaag tggactgaag ggggctttct ttgaggaagc cggaagtaac tttgacggca
    18841 gaccctgggc aaagtcttct ccgccttgag tttgaataac catacttccc cctgactgat
    18901 tcaggactgc tacatcctgg accaaagtgg caccaagatc tacgtgtgga aaggcaaagg
    18961 agccaccaag gttgagaaac aggcggccat gtccaaagcc ctggtaggag ctggagatgt
    19021 gtgacgctga ctgaatattc tgccattatc gatatagaca tgtgctcaaa catgccaggc
    19081 atttgagcac ggtgacacat atgagcttca aagtaaacag gactgcctgg gatatgagtg
    19141 taggttttac cttttccaac tgagtgttct gggcaaggcc actgagcacc tctgagcttc
    19201 agtttcccaa tatctaacag ggatgaaccc tgtctcccag aaccacagta agataaaacc
    19261 agttcgtgag gtactcagaa ctgtactggc acacagaacc tgctctcaga aggtactgtt
    19321 gctattaaag ggggtgtcac atgtccagat gagggcccgg tgctccaggg gcatttacta
    19381 aattctgtcg atccagcacg tgatgtgctt acagggaaca aacgagcaaa taactgatac
    19441 agttagtaga ggcgcatagt aggttcctgc aagatcaatc cataatggcc ataattgtgt
    19501 cagcttaatg aagagacacc aaaaaagtga gtcagaaatt ggcaatgatt cccagggtca
    19561 gagaggagag aatcccacca cgtgacgtca gatggtgaat taccagatga gcaaacactt
    19621 agtaagtccc tgtctgacgt gcgtgggggg tggggggatg tggggactgc aggatttcat
    19681 caagatgaag ggatacccca gcagcaccaa cgtggagacc gtcaacgatg gcgcagagtc
    19741 ggccatgttc aaacagttgt tccagaaatg gtcggtgaag gaccagacca cgggcttggg
    19801 gaaaatattc agcactggta aaatcggtga gatcgcgcca cgtgtgtctt cttcggctgg
    19861 gaccctggaa agaggctcga gtgtctgctc acttggcttt tcccacctct ttctctgcag
    19921 ctaaaatctt ccaggacaag ttcgatgtgt ctctgctaca caccaagcca gaagtggctg
    19981 cacaggagag aatggtggac gacggcaaag gccaggttga ggtgagcccg gtgagggcgc
    20041 actcagcctc tggggtgttt ctgctgcaga ggagggcagc ccgttctgta tgcttccgtc
    20101 cccactccac acttccgtac tgtccaagag gcatggagct ggctgttcac ccacaatgct
    20161 tcaggacagg gagagacact ttccccagtc gtctatcacc tggaagatat gttcaagtca
    20221 tctgtgaagg ccatggccgc ttcactccaa gcttctggct tggtgtgacc tgggacagtt
    20281 ctcaggattc aagtcttgca aatgtgacgg gtcttcagga cacagaggcg gggaagggca
    20341 cttgagatgg atgttagcgc ttcgtcccca cttggggtct gttcaacatc gcccagttcc
    20401 cttccagtgg aattccctga acaggatacc cctctctgag gaattgtgtc tcctcttgtg
    20461 tccaggtctg gagaattgag aacctggagc tggtccccgt ggaataccag tggcatggct
    20521 tcttctacgg gggagactgc tatctggttc tctacacgta cgatgtgaac gggaaacccc
    20581 attacatctt gtacatctgg caggtaggtc aggcgcaagc tctcagcgcg gtattgaatc
    20641 caggggcctc cctgggaaac cccattacat cttgtacatc tggcaggtag gtcaggcgca
    20701 agctctcagc gcggtattga atccaggggc ctccctggga aaccccatta catcttgtac
    20761 atctggcagg taggacaggc aaaagctccg tgtggtgttg gatccagggg cctccctagg
    20821 ctcactcctt tctcaccacc gcagggccgc cacgcatccc gggatgagct ggcagcctca
    20881 gcctatcggg cagtggaggt ggatcagcag tttgatggag cccctgtgca ggtccgggtt
    20941 agcatgggga aggagccacg ccacttcatg gccatcttca aaggaaagct ggttatctac
    21001 gaggtgagga cctacagaca acttcatctt gtgcaaggat gct
//