LOCUS       213839#pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        22654 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 213839
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000055327
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7644..7825
                     /db_xref="ENSEMBL:ENSMUSE00000295821"
                     /rank="1"
                     /note="ENSMUSE00000295821"
     misc_feature    4275..10112
                     /note="5 arm"
     misc_feature    10140..10164
                     /note="B1 Gateway"
     misc_feature    10177..10224
                     /note="Frt"
     misc_feature    10225..10724
                     /note="mouse En2 intron"
     primer          complement(10293..10314)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10189..10212)
                     /note="FRT3"
     primer          14376..14394
                     /note="seq primer NF"
     misc_feature    14436..14443
                     /note="RE sites"
     misc_feature    16236..16269
                     /note="loxP"
     misc_feature    complement(14444..14475)
                     /note="Rox"
     misc_feature    complement(16196..16227)
                     /note="Rox"
     misc_feature    14476..14497
                     /note="polylinker"
     CDS             15056..15652
                     /note="puromycin N-acetyl transferase"
     misc_feature    15171..15176
                     /note="Mutated RsrII site"
     intron          10725..11099
                     /note="mouse En2 intron"
     misc_feature    11097..11099
                     /note="Splice Acceptor"
     exon            11100..11139
                     /note="mouse En2 exon"
     primer          11000..11021
                     /note="seq primer FCHK2"
     misc_feature    11140..11262
                     /note="1Dleader"
     misc_feature    11263..11314
                     /note="F2A"
     misc_feature    11410..12126
                     /note="EGFP"
     misc_feature    11320..11409
                     /note="3X NLS"
     misc_feature    14192..14430
                     /note="SV40 polyadenylation site"
     misc_feature    13225..14169
                     /note="ERT2"
     promoter        14508..15019
                     /note="PGK promoter"
     misc_feature    15763..16195
                     /note="PGK pA"
     primer          14250..14273
                     /note="LRPCR RAF5"
     misc_feature    12136..12189
                     /note="T2A"
     misc_feature    12186..12186
                     /note=" A to G to kill RsRII site"
     misc_feature    12190..13215
                     /note="Cre"
     misc_feature    11115..11140
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    11115..11130
                     /note="FCHK QC prefix region"
     misc_feature    11131..11134
                     /note="FCHK QC critical region"
     misc_feature    11135..11140
                     /note="FCHK QC suffix region"
     misc_feature    16292..16312
                     /note="B2 Gateway"
     misc_feature    10113..16342
                     /note="Synthetic Cassette"
     exon            18285..18600
                     /db_xref="ENSEMBL:ENSMUSE00000390612"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 1 ENSMUSE00000390612 5' fragment"
     exon            17988..18205
                     /db_xref="ENSEMBL:ENSMUSE00001300867"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 2 ENSMUSE00001300867"
     exon            17603..17721
                     /db_xref="ENSEMBL:ENSMUSE00000295808"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 3 ENSMUSE00000295808"
     exon            17149..17286
                     /db_xref="ENSEMBL:ENSMUSE00000179157"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 4 ENSMUSE00000179157"
     exon            16716..16842
                     /db_xref="ENSEMBL:ENSMUSE00000295815"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 5 ENSMUSE00000295815"
     misc_feature    16343..18600
                     /note="Critical Region"
     misc_feature    18624..18657
                     /note="Downstream LoxP"
     misc_feature    18601..18680
                     /note="synthetic loxP region"
     exon            18681..19329
                     /db_xref="ENSEMBL:ENSMUSE00000390612"
                     /rank="6"
                     /note="ENSMUSE00000390612 3' fragment"
     misc_feature    18681..22654
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatctatt gctaattctc aatgttagga ttctctccat ttaagttcga
     4321 caggcctgga acttgctata taggctgacc tcaaattcac agaaatctcc ctgcctcttc
     4381 cttctcacaa gctgggatca caagtgttcg ccacactact atgtctggct ttttctttct
     4441 ttcagtattt ttgtttttat gattatttat tggagtgggg gagggggcat gccacagtgc
     4501 atgtgtggag gtcagagggc atgacctctg acttctcctt ccaccatgta ggtccctgtc
     4561 acctcaggcc tggaggaaag tgctgagtcc actcactgta tttattttct gagcaaaggt
     4621 ttcctgtagc ccaagagtgg ccttgaactc cttgaccctt gggtctccaa tgccccattg
     4681 ctgagatttt aggcttggct cctctctacc tttcatatgc taatccttgt ttctgacagc
     4741 cattctccga ggacaagtcc ccaggactca gctcttccag attaagtcct cctgaaaaat
     4801 gtagtttctt ggtcttctct ggcaaagaat gcatctatac agacgggggg gggggggcac
     4861 gacaccccca tttctcccag ttgtaaggaa gcagggctca ggcttctata cccgtttcca
     4921 gatgattggt tgattgattg attgttttga tgccaggtat caaactcaga accttggaca
     4981 tgctaggtaa ggattttact gttgagccac aacctcagcc cagtttccca ggtactaaat
     5041 gaagcattaa acttgtgtgt gatggttcat gcctgtaatc ccatcacttg agaggtttag
     5101 gtaaaaggtt gtcaagaact ggaggccagc ctgggctaca tagtgagttg aaggccaatt
     5161 tgggccagtt tgtgtgactt tgtctcaaaa acaaacaaac aaaaatcaac caaatcaacc
     5221 aacgaaacaa ataaacaaac aaaaaaccaa aatgaatgaa tgaatgaatg aatgcagcac
     5281 tgggaaggga tggcactcaa tggtagagta catgcttagg atgccatgcc ccaggcctga
     5341 gcagaaagag aaaaagaagg gagggaggga gggagggagg gagggaggga gggagggagg
     5401 gatgcaggca ggcagtactg agagcaatgg tgcctatggt gttcctctga tggatcagac
     5461 atttttacag tttgatgtaa tttctctcca tattttagac tttgagacac agtctttttt
     5521 gtgcaggata ggaactgaaa agagaggagt ctcactctgt agctctggat gtcctggaat
     5581 ttcactacgt agaccaggct gtcctgaaac tcacagatcc acaaacagct gggattaaat
     5641 gttttcacca acatacccct ttcagccatt taaaaaatag ttatttttat tctttttaat
     5701 tatgtgtctg tatgtgggtt tgcacacttg ggccacactt acccacagag accggaggtg
     5761 tagggtctcc tggatctgat ggtgatgatg cctgtgtgga tgctgggaac tgagcttggg
     5821 tcatctataa gaacagttca ctataaacca ccgagccacc tctccagctt tctcttctct
     5881 tctcttctct tctcttctct tctcttctct tcttttcttt ttttgagaca ggcagttagt
     5941 aagttgccca ggctagcctt gaactcaccc agtaagataa ctgctgagtc ccaccctcag
     6001 aacccgtcgg gggccgagtg cagccactac ccctctgcct agcctggagg cgctggtggg
     6061 gaagacgggt gacagttgta aggagggtcc tgtggagctt gaatttgtaa gcttcctgtc
     6121 accctcccaa gtagctggtg aactctctct ttttgagaca gggtcttatt atgaagccta
     6181 ggctaattcc aaatccacaa ttttcctacc ttagctcgtg agtgctggga ttatgggtat
     6241 gtgccatgaa acctggttga gtttaatctc tctctaaaaa aaaaaaaaaa aaaaaagcca
     6301 ggcgtggtgg tgcacgcctt taatcccagc acttgggagg cagaggcagg cgaatttctg
     6361 agttcaaggc tagcctggtc tacagagtga gttccaggac agctagggct acacagagaa
     6421 accctgtcat gaaaaaacaa aacaaaacaa aacaaaaaaa gtaaaaataa ttatgccaga
     6481 tgtggtggct cccgtttttt caaattccgg cccttgggag gcagagacag gtagatctct
     6541 cttagtttaa ggactggggc tacatggtgc gttccaggcc agccagggtt gcaaggtaag
     6601 gccttatcta aaaaagaaag aaagaacgaa agaaagaaag aaagaaagaa ggaaggaagg
     6661 aaggaaagaa ggaaagaaag aaagaaagaa agaaagagag agagagagag aaagaaagtg
     6721 agagaaagaa aggaaggaag aaagaaagaa agaaaaatta atgatgatat acatgtgggc
     6781 acatgcactg ctatgaacgt gtggagttgg ttctttctct tcgggtgggt ttcagggatt
     6841 gaattcgggt tgtcagtctg ccctggcaag cgctcttcct gttgagccta ctcccggggc
     6901 cccagcttaa ttacttaaaa gagtagattt acatcgatgg gagtgggaaa ggaggagtta
     6961 aaaacagctg gaattcacat gatgtagcat gtgacatgtc aagacaacag cagctcagcc
     7021 aagggggaag gtccacaagg agaaagccca ggtatcccgg cccgcacgtg gcgcgtccag
     7081 acggtgtgac caggtcacag gacatatacc atgctaatca acacatgcaa catgtgacat
     7141 gttcccaggg cacacgaagc gctggtgaat tctggttaga actctgcata cacctgccta
     7201 ccagggtcgt cactctgagg tctcttccca ctagaggttt atagcctcct ttaaaaatca
     7261 gccgcccgat tgaagacctg ttctatccag tcatcgcagc aagctggttc ctcactccta
     7321 gcgtgagacg atgcccacaa aggctctctg tgaagggggt tggggtaggg gggtgggggg
     7381 gggtggcgct cgggagaccg cttgctcttc ccgttttcac agtatcccag tcctctcagt
     7441 acaacactca ctcccctaag aatcctgcag agccaaccca cccttaaagc tccacaggac
     7501 cctccttaca actgtcaccc cgcgtcccca ccagcgcctc caggctaggt ggaggggtgg
     7561 tggccgcact ccgcccccgc ggggttccga gggtggggct cagcaggctg agcgtataaa
     7621 gggcgccacc tgcgctccgg gccaccgctc tcggtgcctt gcgctagcta ctttgcactc
     7681 gtacgccgcc ggacctcgcc gctgcctgcc tcgcgccatg ggtcgacaga aggagttgat
     7741 gaatcgttgt ggggagatgc ttcacatccg ctaccggctg cttcgccagg cgctggcgga
     7801 gtgcctgggg accctcatcc ttgtggtgag tgcagggtag tgagcagtcc tatctatttc
     7861 aggccccgtg gtccccaact ccttattcac tccagggaca gttttccagg cagacacgac
     7921 cagttcccca gctctgctcc cgaggcttaa ccctcagggt caagctgacc tccaaagcct
     7981 tcaacagtct tgacagttct aactcaactt gtgacagtgc tacttaccca agaacagctc
     8041 tgctattttg cctttccccc tggagacaga gaccaaggct gcggagggtc accccttagt
     8101 gtgggacctc ccacacgttt cctggcttcc caactttccc agggctccaa cctcaggaca
     8161 cagcagagaa aggcaaatgg acacccctat taagttattt gggtcttggg tgtctggccc
     8221 ctaatgaata attaagcctc agaaagtcca aaagttgcag tgaagtgagg gcggattacg
     8281 gcaggtaggg gcagagggcg cagatttctt gggctgtaag cagcaaaatc ggaagaacgg
     8341 agattgtgga aggaaacaga gcctaacaga acagtgggcg cgtctaaatg tgttgtgcac
     8401 acgctgtagg agtccttcgg atcctgcgac ccagatgctt ccagcgtctt tgctggcctt
     8461 ttgatccccc ctctgcctct cctctcaatg gcccctggtg aacccccaga tctcaggctt
     8521 ctgcagtttt gccccgagtt ctggttaact tggcgcactt cttgatcaga cagacactat
     8581 ggctttcatt tcctgtccca gttactctga ttttcagcca ctgacttgtt tttctgagtc
     8641 tcttctcaat tccggtgtcc ctttttgcag tggggtgggt tacttgagtt ccagcaacag
     8701 cctcagtctt gggtagtgca gatcagtcaa ctggcgaggg aacatggtgg ggcaggctac
     8761 acatggagaa agggggccca aatgtgcctc tgacttcata gactagtggc ctatcagttg
     8821 tccccactcc ctcctgcctc ggtggttccc tgtttcctat cttgatttgt gagtctggcc
     8881 acctgttcgg ttgagagcag caagaggaca agggagggtg tcttacagtt tcaaaatggg
     8941 gccccctcat cccgtcccag ttctcagagc ctattcctct gtaaaagaac agtcagtccc
     9001 cccactgagt cccaggatca cttggcttag cctagaccac aaaaagaggg ttctggattt
     9061 gtggtgaagg ctttcaggct caggtagatg cgagagatca ggtgtcaggg aaaagcttga
     9121 ggagagggga gagcaagcct agtggatccc ctgatgactg agggcctgct cgtgtccctg
     9181 tgttggggac ttgagacctc tgtgtcagga tccaggttga ctttggggtg aggaaagccc
     9241 ccttttttct gaggtatata gttgggtggt atatggggtg aggggctggc taagaacctg
     9301 cagctgggtg aaagtgcagg ataaggaaga ggaaagatta gccccaaggt ggtggaactg
     9361 gctttgacag cctcctcctc cggaggcccc ttggcacgga gccaagtctg ggcctcaggc
     9421 aaagagctca ggccagggtc tgactggaca tagtgcccgg gacggccaca tgcctccacc
     9481 ccttgctctt acaatctctg acctttgctc tcacctgccg gaacaccttt gacctacatc
     9541 gctgccactg gcctccctgc ctcatcttcc cgagtgataa taatcagtgc ttaacaaccc
     9601 agcacccagg cttggaccca ttagagttaa tgaggcacag cttgctgctg gccagcgtag
     9661 cacttacttg tttgtgagac cccgggtctc cttcgcccta gtccttggct gttctagaac
     9721 tcactctgta gaccaggctg gtctctctgc ctcgagagtg cagggatcag gcctggctca
     9781 gcatagcact ggttcagtta tggagaactg agaaaggagt cccagggccg ggctggagga
     9841 gcgtggaggg ccactagaga cttgaggcaa cagcacttaa cagttggtgg agagtattca
     9901 cacttgaaca tatgtctgtc tgtccctgcc gatcctgagg caccttttag tccctctatc
     9961 ccagggttag aacagatctc acgggaccta gagggaagag ctgcttaaaa ccttctattt
    10021 aggactctac agatcagagt ggaaatcctg gagacagcgg gctggagaag gaagaggaaa
    10081 agtggcctgg ggagggaccg gcagggacag ataaggcgca taacgatacc acgatatcaa
    10141 caagtttgta caaaaaagca ggctggcgcc ggaaccgaag ttcctattcc gaagttccta
    10201 ttctctagaa agtataggaa cttcgaaccc tttcccacac caccctccac acttgcccca
    10261 aacactgcca actatgtagg aggaaggggt tgggactaac agaagaaccc gttgtgggga
    10321 agctgttggg agggtcactt tatgttcttg cccaaggtca gttgggtggc ctgcttctga
    10381 tgaggtggtc ccaaggtctg gggtagaagg tgagagggac aggccaccaa ggtcagcccc
    10441 ccccccctat cccataggag ccaggtccct ctcctggaca ggaagactga aggggagatg
    10501 ccagagactc agtgaagcct ggggtaccct attggagtcc ttcaaggaaa caaacttggc
    10561 ctcaccaggc ctcagccttg gctcctcctg ggaactctac tgcccttggg atccccttgt
    10621 agttgtgggt tacataggaa gggggacggg attccccttg actggctagc ctactctttt
    10681 cttcagtctt ctccatctcc tctcacctgt ctctcgaccc tttccctagg atagacttgg
    10741 aaaaagataa ggggagaaaa caaatgcaaa cgaggccaga aagattttgg ctgggcattc
    10801 cttccgctag cttttattgg gatcccctag tttgtgatag gccttttagc tacatctgcc
    10861 aatccatctc attttcacac acacacacac cactttcctt ctggtcagtg ggcacatgtc
    10921 cagcctcaag tttatatcac cacccccaat gcccaacact tgtatggcct tgggcgggtc
    10981 atcccccccc ccacccccag tatctgcaac ctcaagctag cttgggtgcg ttggttgtgg
    11041 ataagtagct agactccagc aaccagtaac ctctgccctt tctcctccat gacaaccagg
    11101 ctcccaggtc ccgaaaacca aagaagaaga acgcagatct ctagagtcac cgagttgctt
    11161 taccggatga agagggccga aacatactgt ccaaggccct tgctggcaat ccacccaact
    11221 gaagccagac acaaacagaa aattgtggca ccggtgaaac agactttgaa ttttgacctt
    11281 ctcaagttgg cgggagacgt cgagtccaac cccggaccgg tgcatgtgga ccccaagaaa
    11341 aagcggaagg tggaccctaa aaagaaacgc aaagtggatc ctaagaaaaa aagaaaggtg
    11401 cacgtgtcca tggtgtccaa gggcgaggaa ctgttcaccg gcgtggtgcc catcctggtg
    11461 gaactggatg gcgacgtgaa cggccacaag ttcagcgtgt ccggcgaggg cgaaggcgac
    11521 gccacatacg gaaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgcct
    11581 tggcctaccc tcgtgaccac actgacctac ggcgtgcagt gcttcagcag ataccccgac
    11641 catatgaagc agcacgactt cttcaagagc gccatgcccg agggctacgt gcaggaaaga
    11701 accatcttct ttaaggacga cggcaactac aagaccaggg ccgaagtgaa gttcgagggc
    11761 gacaccctcg tgaacagaat cgagctgaag ggcatcgact tcaaagagga cggcaacatc
    11821 ctgggccaca agctggagta caactacaac agccacaacg tgtacatcat ggccgacaag
    11881 cagaaaaacg gcatcaaagt gaacttcaag atccggcaca acatcgagga cggctccgtg
    11941 cagctggccg accactacca gcagaacacc cctatcggcg acggccctgt gctgctgcct
    12001 gacaaccact acctgagcac ccagagcgcc ctgagcaagg accccaacga gaagagggac
    12061 cacatggtgc tgctggaatt cgtgaccgcc gctggcatca ccctgggcat ggacgagctg
    12121 tacaagcctg caggcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
    12181 cccgggccgt ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca
    12241 acgagtgatg aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct
    12301 gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg
    12361 aataaccgga aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt
    12421 caggcgcgcg gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt
    12481 catcgtcggt ctgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg
    12541 cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa
    12601 cgcactgatt tcgaccaggt tcgttcactc atggaaaata gcgaccgctg ccaggatata
    12661 aggaatctgg catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc
    12721 aggatcaggg ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc
    12781 agaacgaaaa cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact
    12841 aaactggtcg agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg
    12901 ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact
    12961 cgcgccctgg aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac
    13021 tctggtcaga gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat
    13081 atggcccgcg ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta
    13141 aatattgtca tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg
    13201 ctggaagatg gcgatctcga gccatctgct ggagacatga gagctgccaa cctttggcca
    13261 agcccgctca tgatcaaacg ctctaagaag aacagcctgg ccttgtccct gacggccgac
    13321 cagatggtca gtgccttgtt ggatgctgag ccccccatac tctattccga gtatgatcct
    13381 accagaccct tcagtgaagc ttcgatgatg ggcttactga ccaacctggc agacagggag
    13441 ctggttcaca tgatcaactg ggcgaagagg gtgccaggct ttgtggattt gaccctccat
    13501 gatcaggtcc accttctaga atgtgcctgg ctagagatcc tgatgattgg tctcgtctgg
    13561 cgctccatgg agcacccagt gaagctactg tttgctccta acttgctctt ggacaggaac
    13621 cagggaaaat gtgtagaggg catggtggag atcttcgaca tgctgctggc tacatcatct
    13681 cggttccgca tgatgaatct gcagggagag gagtttgtgt gcctcaaatc tattattttg
    13741 cttaattctg gagtgtacac atttctgtcc agcaccctga agtctctgga agagaaggac
    13801 catatccacc gagtcctgga caagatcaca gacactttga tccacctgat ggccaaggca
    13861 ggcctgaccc tgcagcagca gcaccagcgg ctggcccagc tcctcctcat cctctcccac
    13921 atcaggcaca tgagtaacaa aggcatggag catctgtaca gcatgaagtg caagaacgtg
    13981 gtgcccctct atgacctgct gctggaggcg gcggacgccc accgcctaca tgcgcccact
    14041 agccgtggag gggcatccgt ggaggagacg gaccaaagcc acttggccac tgcgggctct
    14101 acttcatcgc attccttgca aaagtattac atcacggggg aggcagaggg tttccctgcc
    14161 acagcttgat gaagatctga gctcttaatt aatgatcata atcagccata tcacatctgt
    14221 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    14281 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    14341 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    14401 caaactcatc aatgtatctt atcatgtctg gatccgttta aactaacttt aaataattgg
    14461 cattatttaa agttacatat ggcggccgcc ctaaggctcg acctcgaaat tctaccgggt
    14521 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
    14581 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
    14641 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
    14701 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
    14761 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
    14821 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
    14881 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcttccgg
    14941 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
    15001 atctccgggc ctttcgacct gcatccatct agatctcgag cagctgaagc ttaccatgac
    15061 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
    15121 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cagatcgcca
    15181 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
    15241 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
    15301 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
    15361 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
    15421 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
    15481 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
    15541 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
    15601 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
    15661 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cgatgatatc
    15721 agatccccgg gatgcagaaa ttgatgatct attaaacaat aaagatgtcc actaaaatgg
    15781 aagtttttcc tgtcatactt tgttaagaag ggtgagaaca gagtacctac attttgaatg
    15841 gaaggattgg agctacgggg gtgggggtgg ggtgggatta gataaatgcn tgctctttac
    15901 tgaaggctct ttactattgc tttatgataa tgtttcatag ttggatatca taatttaaac
    15961 aagcaaaacc aaattaaggg ccagctcatt cctcccactc atgatctata gatctataga
    16021 tctctcgtgg gatcattgtt tttctcttga ttcccacttt gtggttctaa gtactgtggt
    16081 ttccaaatgt gtcagtttca tagcctgaag aacgagatca gcagcctctg ttccacatac
    16141 acttcattct cagtattgtt ttgccaagtt ctaattccat cagaagctgg tcgagtaact
    16201 ttaaataatt ggcattattt aaagttaggc gcgccataac ttcgtatagc atacattata
    16261 cgaagttatg tcgagatatt taaatattag acccagcttt cttgtacaaa gtggttgata
    16321 tctctatagt cgcagtaggc ggattcaact tctcttttga tctctctccg tggcccccac
    16381 ctcctccact atgacctggg ctttggaaga gtccaggacg atggggtatt tcctacctct
    16441 gtgtgccaaa tctggaggac gggcagaaac tgctgtagtg atagctccct tctgtgccct
    16501 tcccggattc aagaaaaacc agtggatgtc atttaaccac ctgggtcccc agtcatgtac
    16561 ccattactga ttccccccca tccccccatg aggctctgca cgtctcctct ttctgggact
    16621 taaggaggga tttgggttat atcccaggaa gcaccttcag tggtgtcagg catgtctgat
    16681 ctcagtggga cttcacttgc tttgttttcc gacagatgtt tggctgtggc tccgtggctc
    16741 aggtggtgct cagccgtggc acccatggtg gcttcctcac catcaacttg gcttttggct
    16801 tcgctgtcac ccttggcatc ttggtggctg gccaggtgtc tggtaaggcc tcaaccccag
    16861 cttcagtttt cagccctcac cagcatttcc aacaagtatc tgcctagaga gcagaggggg
    16921 aggaacaact ccaaccaagg acgcacacta acagcacctc agctttgggc cctttggagg
    16981 caagggtgga agagacttca cagaaccttt gactttcacc ttggaatcaa agatcagtct
    17041 gtgacatagt ccaactgcca tccaggctaa gaaaacctac ctacacccca gaaaggaaga
    17101 gcccaaggtg gcaggctgta actaatggat gctatctcct cttcgcaggt gcccacttga
    17161 accccgctgt gaccttcgca atgtgcttcc tggcacgaga gccctggatc aagctgccca
    17221 tctatgcact ggcacagaca ctgggggcct tcttgggcgc tgggattgtt tttgggctgt
    17281 actacggtaa gcattcccca tcccgccctc ccttctccac actttccctc tttaagtact
    17341 tgttggcacc aagcccactg atgacaaccg gggcctgccc aggcccaggg cccgtgactc
    17401 attcacgaac actcaggccc aggttggggg cctaggggaa agaaacgagt tgggcaacaa
    17461 cagaatctca ggtcctccac cccgccccac cccctgagcc tctacagtca tatgcttacc
    17521 catgacccct ggcggggtgg ggagggcagc tctgagagga gaggctctgc cctcactcac
    17581 aatggctcta atctgtcacc agatgcaatc tgggcctttg ccaacaatga gcttttcgtc
    17641 tctggcccca acggcacagc tggaatcttt gccacctatc cctctggaca cttggacatg
    17701 gtcaatggct tctttgatca ggtatggact agggacatgt gaagtaaagg tagagggagg
    17761 aacagtcttg ttttggacaa cgctccttga ttgtagcagg atttcttctc agttcgtgag
    17821 aaccccaacc tcagaacatg gtggtggcgt tgtcttttat aaagcatggc gcttcccagt
    17881 gagttcttgg ttttgcgatc atcctagagt caggatggtc tctagcgtgg aggactggag
    17941 cgtgggagaa ggagctggcc ctcaccgtgc tctctcccct ctcccagttc ataggcacag
    18001 ccgcccttat tgtgtgtgta ctggccatcg ttgaccctta taacaaccct gtgccccgtg
    18061 gcctggaggc tttcactgtg ggcctggtgg tcctggtcat tggaacctcc atgggcttca
    18121 attctggcta tgccgtcaac cctgcccgtg actttggacc tcgcctcttc accgccctgg
    18181 ctggctgggg ctcagaagtc ttcacgtgag tacagtcccc actccccagc ttgcctcccc
    18241 tcttcctgcc gacctgtctc tgatttccgg tgttctccct ccaggactgg ccggcactgg
    18301 tggtgggtac ccattgtctc cccactcctg ggttccatcg ctggtgtctt cgtgtaccag
    18361 ctcatgattg gttgccacct ggagcagccc ccaccctcca ccgaggaaga gaatgtgaag
    18421 ctggcccaca tgaaacacaa ggagcagatc tgagtgggca gcagcccccc tcccccactg
    18481 tgcactctcc tgagtgtcca ctgactgtgt ggggaccagt ccccgaaagc cctttgtgat
    18541 gcctctctcg ggctaaaccg ctccctgtgt ccacccctgc tggatgggcc ctccagaatt
    18601 gagatggcgc aacgcaatta atgataactt cgtatagcat acattatacg aagttatggt
    18661 ctgagctcgc catcagttca attagggcat taggttccca cccaccttta agccaaggta
    18721 ggatagcaaa taagatggag agagagagag agagagagag agagagagag agagagagag
    18781 aatgaatgtg tacatgtgtg ctgttttcta agctgaatga tgcaaaggca agggaccaag
    18841 ttttcaaaac aaactgtagc agctcagggg aagggagccc aggggaaggg agaaagtgag
    18901 tcaggaatgt gccagagtgt gcatgcttca gggactcctc catgtggagg tggacccaga
    18961 agtgagtttc taagtatgcg tgtgcctact gttttttttt ttttttttga aatggacttc
    19021 taggcttggg gagggggaag ggataagaag ggtgtagctc acatctggag ctatgaccct
    19081 tgactggggg ctgtgtaata tgtttctgtt ataagataga cattgggagg ggctgaagtc
    19141 caggtcgtaa gtttcataat ttgtttttta aatatataaa tatatacata catatatgtt
    19201 acagccctag gaataggggt gggaaactcc actttttaaa aggggtttcc tttctttaat
    19261 cctccaatca acaatgtact gttgcctttt atatataaaa aagaataaaa cgtatacatg
    19321 ctacaggaac ttctttttgt gtgtgttttt tgttttcaaa cagggtctct agtcctggct
    19381 atcctgggac tctgtatacc aggctggcct tgaactcatg gatacccccc tgctgacatt
    19441 aaaagcatgt gccaccacac cagagtcttg tttttctggg aatagcattc gagcgttggt
    19501 tttatttggc cataaatttc cagatgttta gttcagagtc taatcttagc ttgtattcag
    19561 tggtctcaga ttccatccaa tggagcatct tccgaatcct cctgtctcat ccccttccac
    19621 aaggaggaag tttggagccc ctctggtagg cagagccacc cagagactgg agaggctaag
    19681 cctatggctc aaagaggcca gtcagactgg ggacctgtat tgtcctctag agttgctaaa
    19741 tctccatcca gactcttggc tgtggcactc ttatcagcct ccctccaagg agtcagtcca
    19801 cacaggttcc tcagggtatc ttcctgtgga taggtctctt tccatggtcc tcaccacatc
    19861 tccacagtcc atctgcatcg ggttctcttg tagctgtcct tacccaccct gtcacaacgg
    19921 tctcccttca ctcctggcta ctttctctct ttcctatccc tcttctagga aatgggttac
    19981 tcctattctt catcttccaa cctaaaagcc acttgcctgg atcaagaacc tctcctgttg
    20041 ccttctggga catgtccgtg cccagtaagg caagggttat gtgactagcc tggcacacct
    20101 gagcccagga agtgcgcctc atttccagct tccttaatac atctgactta aatttcacca
    20161 ggaatataag ggaaaccttt ctgcagcgtt aaccctgtag gagccaggag atccccggcc
    20221 tgcataatta tgcccttaag gctcttgctc agtagcccct tcacagatga gacccatgcc
    20281 cagaaagggt gacctgcaag ttcaggccac agaactaggg ttgtgaaagg agtccctggg
    20341 acaagacaat tccttcgtgg atctccaccc tttccttatt cccaacttcc aagaggcatc
    20401 tctggaatgc tggagagacc tgagccagag ggagtgtccc tttcccagac cccgaggggg
    20461 aagaacgtta accttgagag cagaaggtgc ctgtgaagtt tccttcagtg taccaagcag
    20521 tttcattcag ccacaccagt tcccccttgg cccccatcca agaccctgcc tcagcatcct
    20581 tcttttcagc caatggggag caggaagctt gagatgccat cactataggg tagtgtccat
    20641 cacagctact aggtgtgttt gtgtgccttc tctggacctt agtttcttta tctgtgacac
    20701 tcgggttaat aaaaatggta aataggcaga gatgattcat agaaagggct taacgtaggc
    20761 tggcgagatg cctcagtggg taatggcacc tgccgtccag tctggcaagc taagtctgat
    20821 ctctggaacc caaagactag aagaaagaac caattctcca gaaagttgtc ctctgaacgc
    20881 cacacttgca ctctggcctg ctgatacata tacatacacg cgcgacagta aatgtaattc
    20941 agaatgtaag tgagctaagc agtgaccagg aaacaaataa taaatggggg ctcatgatgg
    21001 ctttacctgc agaaccagga tggggcagga aagaaggagt ggaggtgggg agctgttttg
    21061 taaagaccat tggatttaac gagcaccttt cgtctcagat ccatccacaa gtgtggattt
    21121 actgaaaggg gagtgaggaa gtggggtggt ggcttgcccc gctgtgtcct gaccctacga
    21181 ggctcttggg ttgggtagta aggaattggt ataagaaagg caagacagag ggagccacga
    21241 gtgtgtgggg tggaggacgg aacatgagct aggcagccac tctgggactc ctccagataa
    21301 ggagttcccc tttaatcctc cacttggaat aggcaaagtc gtgactatta ccaaattctg
    21361 gaaggaaaat tccttacttc ctataagaaa gcttctccat ttcacagaat ttattttcag
    21421 ggaagccgga ggagggggga gggaattgtc ccacggtgtg gctgctttgt cccagggctt
    21481 gccaagggct ttttatgtgt ctgtaccaag ccctcaccac aaccatttta aagagccagt
    21541 tttcttctct ctgtctgtct ggtcccgtcc cccccccccc cgcttcattt ttcgagacag
    21601 ggcttctcta tttagctcct ggaactcact ctatagacaa gactgtcctg gaactcatat
    21661 agatctgcct gtcgctgcct ccagagtgct gggattaaag gtgtgtgcta ccactgcctg
    21721 gctggagcca tgtttcttaa agccgatgat ttgcaccagt gaaatcagct ccctgaaaca
    21781 tgagctgttt ggatacctgg ccctgtgtct gtgcacacag ggtgcacaca gccctgtgtt
    21841 tctcagggct ccttgtgggc ctgtttttct ctcacaatgt tgggctgtct gaatctctag
    21901 ttttcttcat ctttggcttt ctctgtgtgt taccgcctaa gtcttcattt acctcttaag
    21961 aggcatctct gtatgtaccc tgagtccatt tctctgtgcc cttggactgt acctctaagt
    22021 gcaagtaaca ccagccaacc atctctgctc cagggcatgt cttctgaatt cctggtaatg
    22081 agctctggct tgctgctaac tcttatagat tttgtttttt tttttttttc tgaaaaacct
    22141 gggtggggca tccacaagct tggagaaact tgggtgaggt agagttgggt ggggtgggaa
    22201 ggcaagaaga aggccaactc cacccagtat agaaacgcta gagcaggagt tctcaatctg
    22261 tgggtcgtga cctctatgag gctacattgt gcacaccttg cttgtcagat atttacttac
    22321 aattcttcac agtaacaaaa atacagctat gaaatagtaa cgaaatactt ttatggttgc
    22381 aggtcatgac agcatgagga actggattag acggttgaag catcagggag gttgagaacc
    22441 actgccctaa agcctaggga agatatggtg accccaagga tctaggagga ggctggctga
    22501 ggaagggagg agcctgtgct gggagtgggg agcacgggag ctgtctggag gaagagagga
    22561 tgtgtgaagg aaggaggggc tgagatttac ccacccctta gacctaaaag ggtcttgaca
    22621 gtggaatgac tgagccctaa aaggtcaggt atct
//