LOCUS       230507#pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20073 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 230507
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000105229
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7651..7784
                     /db_xref="ENSEMBL:ENSMUSE00000665397"
                     /rank="1"
                     /note="ENSMUSE00000665397"
     exon            9127..9203
                     /db_xref="ENSEMBL:ENSMUSE00000150024"
                     /rank="2"
                     /note="ENSMUSE00000150024"
     misc_feature    4275..10134
                     /note="5 arm"
     misc_feature    10162..10186
                     /note="B1 Gateway"
     misc_feature    10199..10246
                     /note="Frt"
     misc_feature    10247..10746
                     /note="mouse En2 intron"
     primer          complement(10315..10336)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10211..10234)
                     /note="FRT3"
     primer          14398..14416
                     /note="seq primer NF"
     misc_feature    14458..14465
                     /note="RE sites"
     misc_feature    16258..16291
                     /note="loxP"
     misc_feature    complement(14466..14497)
                     /note="Rox"
     misc_feature    complement(16218..16249)
                     /note="Rox"
     misc_feature    14498..14519
                     /note="polylinker"
     CDS             15078..15674
                     /note="puromycin N-acetyl transferase"
     misc_feature    15193..15198
                     /note="Mutated RsrII site"
     intron          10747..11121
                     /note="mouse En2 intron"
     misc_feature    11119..11121
                     /note="Splice Acceptor"
     exon            11122..11161
                     /note="mouse En2 exon"
     primer          11022..11043
                     /note="seq primer FCHK2"
     misc_feature    11162..11284
                     /note="1Dleader"
     misc_feature    11285..11336
                     /note="F2A"
     misc_feature    11432..12148
                     /note="EGFP"
     misc_feature    11342..11431
                     /note="3X NLS"
     misc_feature    14214..14452
                     /note="SV40 polyadenylation site"
     misc_feature    13247..14191
                     /note="ERT2"
     promoter        14530..15041
                     /note="PGK promoter"
     misc_feature    15785..16217
                     /note="PGK pA"
     primer          14272..14295
                     /note="LRPCR RAF5"
     misc_feature    12158..12211
                     /note="T2A"
     misc_feature    12208..12208
                     /note=" A to G to kill RsRII site"
     misc_feature    12212..13237
                     /note="Cre"
     misc_feature    11137..11162
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    11137..11152
                     /note="FCHK QC prefix region"
     misc_feature    11153..11156
                     /note="FCHK QC critical region"
     misc_feature    11157..11162
                     /note="FCHK QC suffix region"
     misc_feature    16314..16334
                     /note="B2 Gateway"
     misc_feature    10135..16364
                     /note="Synthetic Cassette"
     exon            16493..16634
                     /db_xref="ENSEMBL:ENSMUSE00000150026"
                     /rank="8"
                     /note="ENSMUSE00000150026"
     misc_feature    16365..20073
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaataatt cataaaactg cttcttagct taggtgattg cctgatcttt
     4321 gcattttctt ttatagaaga tttacatctc gtgggaagtg aagtgagtgt gtaaggggca
     4381 gatgaggtac tgaataatgt gtattattga catgggcaca cccgtggctt ttttgtttgt
     4441 ttgtttttgt tttttttttt ccttgaacca gggactctat gtagcctttg ttgatttgga
     4501 attccaggct ggcctggaac tcagagctcc acctgcctct gcctcctgtt gctgggatta
     4561 aaggtgtgca caactatctc ctggatttat cacaacgttg ggactagtgg cttatttaac
     4621 ataccaaatg gacctgggct ccacattctc ccgctctccc tcagtctcta cctattatag
     4681 gctgggatgc ccggccctct atgtgaaact ttccaaccca ggggctgggc ttcctctcgc
     4741 ccatccgagt gtgtgtgtgt gtgtgtgtct ccctctcccc tctgtctctc tgtctctctt
     4801 tctctctgtg tgtgcttgag ttctcttcct cttccccttc ccttttgtct cccccaagag
     4861 ctacccccac aaacctgctt tcatacttta atttggctta aattggctca tttcgttggc
     4921 agagagtaac ctatcacatg acatttccat acatgaatat ttaccctgtt tttccttttc
     4981 ctattcatcc cctgttctct tttccctccc acgtcactaa taataattct ccatttactt
     5041 tcatgtcctt ttaaaaagcc gttccggagt ccacatgtga gagaaaacat gtgatgtctg
     5101 tctttgtgac cacggtgacc tctggttccg tttgttttca tgcatgctac atctctctgg
     5161 atgtgtggtg tttgtctatg tgtgtgaggt tagagaagga agttaggtgg ccagctttat
     5221 catcttccac cttggtccct tgagactgga cttggagcta ggctgatggc cagcaagtcc
     5281 cagtgatccc cttgtctcag ttctgagatg cttgtggagc tacacctgat ttttaaaaaa
     5341 atgtaatttt atttattttg tttttgtatt ttggaatatt tatttattat ttgcttatgt
     5401 atttaaatta cttttattct tttacaatcc tcccattccc ttcctgttct cacccccaca
     5461 gttgctcatc ccgttcctcc tcccatctcc aaaaggatgt ccccaccacc accataattt
     5521 tattttttgc ttattcactt tacatcctgc tcgctggtca cccctgtcca catagtcctt
     5581 gcccttcccc ctccccttct cctctgagtg gtggaggccc ccccaccata tcctgccacc
     5641 cacacacctg attttttttt ttttttttta tgtgggtgct tggatcagaa ctcaggtcct
     5701 tatgtttgtg caagtgctct tacctactga gtgatctttc caacccttag gatacataat
     5761 tttaagaata tatgcgtgta tatgtgcgtg tatattttgc ctgcatgtat atatgtgtaa
     5821 tatatatata tatatatata tatatatata tatatatatg tgtgtgtgtg tctgatatat
     5881 gcccaagaag gcaagaaaat ggcatcatat ccactggaat tggagttaca gaggtcatag
     5941 gccaccatgt gtatgctggg aattgaagtc aggcactctg taagagcaag tgctctaaac
     6001 aactgagcca tctatctccc cagccctttc tcctcctctt ccccctcttc ctccttcccc
     6061 tcttcctcct ccttttcttt ttcttcctcc tcctttttct tttcttcctc ttcctctttc
     6121 tcctttctcc tcttctgcct cctcttcctc ctctttcttt tctttttcct cctcttcgtt
     6181 ctccttttcc tcttcttctt cttcctcttt cccttcctct tcttcctctt ttttcttttt
     6241 cctcttcttc cttctccttt tcctcttctt cttcctcctc tttctcttct tcctcctcct
     6301 cttcctcctc tttcatttct ttttcctcct cttccttctc cttttccttt tcttcttcct
     6361 tttctttctc ttcctcttcc tcctcctcct ttttgagaca gggtcccagc atatggccca
     6421 agctggcctg taagtcatga ttgctctact tcagcctgag tatggaaata ataaacatgt
     6481 gctatcacgc caaggttctt catttatctg gtactgattt attattcgat tatttactca
     6541 tatttggaga caaggactaa ttccttagac tggcctcaaa ctccttaggt agccaaggat
     6601 gatcttgaat tcatgagact tatgcctcta cctcatgagt tctggaataa taagcacata
     6661 ccacaaccat gcctggttca tgaggtgcta agaatacaag cccaggctgt ctgcatatta
     6721 agcaagcact ttaccaactg aactacgtcc tcagctcagg tttattcttg ttttttttga
     6781 gatggggtct cgagtcgtag ctcattctgg ccttgaactt tgaatgacca agggatcctc
     6841 ccgtttcagt ctcccaaatg ctatacttat aggtgtatac cactgatttt gttttggttg
     6901 gattgagaca aggtgttgtt gtgtagccca ggatggcctg taaccttcag ccatcctgcc
     6961 ttcacctcca tagtgctggg aaggacaggc ctcaccatca tgcctagact tctttgtagc
     7021 ctgggaactg agcctgagtt cgtgacacat gctctaccat agatctgcat gcatttccat
     7081 tcgccatttt gaactatagt ctcactagca ctgggtatac agcttagctg gtagagtgct
     7141 tgcctagtag gcacaaaatc ctgggtctgg tgccagaact gcataaacta gatgcacttg
     7201 tgcacaccgg tggtgccagc acttgtggag gcaaaatgac ctgcagttca gggttatcct
     7261 cagctacata tggaattcca ggccagcctg ggctacatga gaccctgatt ccaaagagat
     7321 aggtgtgtgt gtgtgtggtg tctcactaaa ctgtccagag tggctttgaa tttttgattt
     7381 tcttgtgcca ggtgtggtgt catggacttt tagtgtctgg aggctgaggc aggcagatct
     7441 ctgagtttga ggccagcctg gtctacagat agagttccag ggcagccggg tctacacaga
     7501 gaaaccctat cgagagagag agagagagag agagagagag agagagagag agagagagag
     7561 aatatgaaag tgctgggatt acatatgtgt gccaccagct ggcctttgtt ttaattgttt
     7621 ctttatatac agtcccttcc cttcagattc agtaagcaac tcaaggcaga aggagctgcg
     7681 gagaaaagga caaagttcag gttaggagtg taaggccggt cggtcctatc tgagcttttt
     7741 acaacagttc cttctgtgaa ccaccagcgg cacacgggag aaaggtattt ctaactctcg
     7801 cctttacctg gttttgccat ctctgatgaa aagaggaata agatacggaa gtccatgcta
     7861 ttgcctcgcc ggccttagtg tcctttctca catctccatt aaccactggc taatccagga
     7921 gcaagggagc tctaaggctt tgttaatctt ttcccaggag ggcccaggct ctgacgtgtt
     7981 atgattgacg catggaaacg gggttctaga caagggcttc ctcaaagggt tttctcagct
     8041 caggagttct agaaaatgga gaagctgggg ccataaaacc ctagtccaga atgttgggag
     8101 acttctagga tgggaagcta ggggctttct ggaagtgttt attctgactt cacccagtgc
     8161 cactcaaccc acaccttctg cgggtggctc gggaatgggg cggggggcag ggccttgaac
     8221 aaggacggag agggagctgc gtggtgggta ctcggtgggt tgaggtctgc agatgaccgt
     8281 tttgatactt tggctcagtt ctgtctttgg gcaactctga gccgccgtgc gtcctctgga
     8341 ctctgactcg cctactcctg aaccagagag aaccagacgg cgcctgggtt gcagagggct
     8401 ccgggaaccc tgaggtccgg ctcctggctc gagccttcca ggcagcggcc acgtggcctt
     8461 gtcctggcgg ggggctcacc tccccctgct agcctggtga ctcaggccgc agctgttccc
     8521 gtgtcacatg agagagagag ggagggaggg aagaaggcgg ccaccctgcg ggggaggggg
     8581 gcggccggag ccggggcggg gccgtgcagc aggcggggcg cgggcagggg gcacgggaga
     8641 cggcggctga gccgcggcag gagagtactg agaagcgtcg gggagcgcag gtgagccggg
     8701 cccggggacc cgggggacgt ccgtgggggc aactttgcct cgaagttcgc ggggcgccat
     8761 ccgcgccgca cccgagggga ggctcgcgcc caggggctgg cgagggtctc gggtcgcggc
     8821 ctgtcgggat cggggatcgg gaatcggcgc tgtgcagcgg cgggcggaga tctacctgga
     8881 gtccggccgc gccctccgtg cgtcccggga tcccgagatc ccgaggcccc gctgcgtggc
     8941 gcgccccagt ccggccggcg agcggggagg gctgggagac gtgggtctga ccgcgggttt
     9001 ctgggtggga gggcagcgat gggggccgcg cagggaaggc tgcgaaggcc ggcggggagc
     9061 tgcggatgtg gcggcccctc ggcctgctct acctgctcta atccttggtg ttctccgcgg
     9121 ccccaggccc aacagccatg gcggtggaag gaggaatgaa gtgtgtcaag tttttgctct
     9181 acgttctcct gctggccttc tgcgtgagta gtgcggggag agcgacgggg cgcccgggcg
     9241 gggtgcgggg gggggagtct tggagcggga gccgtgaagg gtgagggacc cagagatgaa
     9301 ggcagctgga ggccagatgt ggaaggaaag cccagggaaa agggccaaag gcagaacgtg
     9361 tccgcgctac ctcccttccc catcccccac gcgcagcttt ccgggacctc tgagtaaagt
     9421 ttggaccgag aattgccatc ctagacgggg agcaccccca accaaccccc tcctggccct
     9481 tcctgtctgg tgacccaacc attcgtggta cggggtggtg gggcaacctg ccaacggtct
     9541 gatggtatcg ccaaggggct gtctccactc ctcacaccgt tgattcctct ccattctctt
     9601 agccctctca tttacttggg ggtaggaaag gctctgccct ccttcatttg ctcctccctg
     9661 tgtgttctga aaggggccac tctttttttg tttgtttgtt tttcgagaca gggtttctct
     9721 gtatagccct ggctgtcctg gacctcattt tgtagaccag gctgacctcg aactcagaaa
     9781 tccgcctgcc tctgcctccc gagtgctggg attaaaggcg tgcgccacca cgcccggccc
     9841 gaaaggggcc actcttaccc cctcctccgc cctagcctag ctggccctag gacctgagtc
     9901 actggagtga gggggtagtt ctgggagaga ctctagctgg gagtccatag aaagtaaccc
     9961 atatcctgtg ccgaagcgct gtttaaaggc agcatgacac ctcccgattc ccttctcctt
    10021 gtgtgcctta aacaacttcc cagccttcat tgtggggcaa ggatgggcag accttgtcta
    10081 cagccagtgt ttcctggagg tgtaggatct atcttctggg tgggggtcgg gtgcaaggcg
    10141 cataacgata ccacgatatc aacaagtttg tacaaaaaag caggctggcg ccggaaccga
    10201 agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcgaac cctttcccac
    10261 accaccctcc acacttgccc caaacactgc caactatgta ggaggaaggg gttgggacta
    10321 acagaagaac ccgttgtggg gaagctgttg ggagggtcac tttatgttct tgcccaaggt
    10381 cagttgggtg gcctgcttct gatgaggtgg tcccaaggtc tggggtagaa ggtgagaggg
    10441 acaggccacc aaggtcagcc ccccccccct atcccatagg agccaggtcc ctctcctgga
    10501 caggaagact gaaggggaga tgccagagac tcagtgaagc ctggggtacc ctattggagt
    10561 ccttcaagga aacaaacttg gcctcaccag gcctcagcct tggctcctcc tgggaactct
    10621 actgcccttg ggatcccctt gtagttgtgg gttacatagg aagggggacg ggattcccct
    10681 tgactggcta gcctactctt ttcttcagtc ttctccatct cctctcacct gtctctcgac
    10741 cctttcccta ggatagactt ggaaaaagat aaggggagaa aacaaatgca aacgaggcca
    10801 gaaagatttt ggctgggcat tccttccgct agcttttatt gggatcccct agtttgtgat
    10861 aggcctttta gctacatctg ccaatccatc tcattttcac acacacacac accactttcc
    10921 ttctggtcag tgggcacatg tccagcctca agtttatatc accaccccca atgcccaaca
    10981 cttgtatggc cttgggcggg tcatcccccc ccccaccccc agtatctgca acctcaagct
    11041 agcttgggtg cgttggttgt ggataagtag ctagactcca gcaaccagta acctctgccc
    11101 tttctcctcc atgacaacca ggctcccagg tcccgaaaac caaagaagaa gaacgcagat
    11161 ctctagagtc accgagttgc tttaccggat gaagagggcc gaaacatact gtccaaggcc
    11221 cttgctggca atccacccaa ctgaagccag acacaaacag aaaattgtgg caccggtgaa
    11281 acagactttg aattttgacc ttctcaagtt ggcgggagac gtcgagtcca accccggacc
    11341 ggtgcatgtg gaccccaaga aaaagcggaa ggtggaccct aaaaagaaac gcaaagtgga
    11401 tcctaagaaa aaaagaaagg tgcacgtgtc catggtgtcc aagggcgagg aactgttcac
    11461 cggcgtggtg cccatcctgg tggaactgga tggcgacgtg aacggccaca agttcagcgt
    11521 gtccggcgag ggcgaaggcg acgccacata cggaaagctg accctgaagt tcatctgcac
    11581 caccggcaag ctgcccgtgc cttggcctac cctcgtgacc acactgacct acggcgtgca
    11641 gtgcttcagc agataccccg accatatgaa gcagcacgac ttcttcaaga gcgccatgcc
    11701 cgagggctac gtgcaggaaa gaaccatctt ctttaaggac gacggcaact acaagaccag
    11761 ggccgaagtg aagttcgagg gcgacaccct cgtgaacaga atcgagctga agggcatcga
    11821 cttcaaagag gacggcaaca tcctgggcca caagctggag tacaactaca acagccacaa
    11881 cgtgtacatc atggccgaca agcagaaaaa cggcatcaaa gtgaacttca agatccggca
    11941 caacatcgag gacggctccg tgcagctggc cgaccactac cagcagaaca cccctatcgg
    12001 cgacggccct gtgctgctgc ctgacaacca ctacctgagc acccagagcg ccctgagcaa
    12061 ggaccccaac gagaagaggg accacatggt gctgctggaa ttcgtgaccg ccgctggcat
    12121 caccctgggc atggacgagc tgtacaagcc tgcaggcgag ggcagaggaa gtcttctaac
    12181 atgcggtgac gtggaggaga atcccgggcc gtccaattta ctgaccgtac accaaaattt
    12241 gcctgcatta ccggtcgatg caacgagtga tgaggttcgc aagaacctga tggacatgtt
    12301 cagggatcgc caggcgtttt ctgagcatac ctggaaaatg cttctgtccg tttgccggtc
    12361 gtgggcggca tggtgcaagt tgaataaccg gaaatggttt cccgcagaac ctgaagatgt
    12421 tcgcgattat cttctatatc ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca
    12481 tttgggccag ctaaacatgc ttcatcgtcg gtctgggctg ccacgaccaa gtgacagcaa
    12541 tgctgtttca ctggttatgc ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc
    12601 aaaacaggct ctagcgttcg aacgcactga tttcgaccag gttcgttcac tcatggaaaa
    12661 tagcgaccgc tgccaggata taaggaatct ggcatttctg gggattgctt ataacaccct
    12721 gttacgtata gccgaaattg ccaggatcag ggttaaagat atctcacgta ctgacggtgg
    12781 gagaatgtta atccatattg gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa
    12841 ggcacttagc ctgggggtaa ctaaactggt cgagcgatgg atttccgtct ctggtgtagc
    12901 tgatgatccg aataactacc tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc
    12961 tgccaccagc cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt
    13021 gatttacggc gctaaggatg actctggtca gagatacctg gcctggtctg gacacagtgc
    13081 ccgtgtcgga gccgcgcgag atatggcccg cgctggagtt tcaataccgg agatcatgca
    13141 agctggtggc tggaccaatg taaatattgt catgaactat atccgtaacc tggatagtga
    13201 aacaggggca atggtgcgcc tgctggaaga tggcgatctc gagccatctg ctggagacat
    13261 gagagctgcc aacctttggc caagcccgct catgatcaaa cgctctaaga agaacagcct
    13321 ggccttgtcc ctgacggccg accagatggt cagtgccttg ttggatgctg agccccccat
    13381 actctattcc gagtatgatc ctaccagacc cttcagtgaa gcttcgatga tgggcttact
    13441 gaccaacctg gcagacaggg agctggttca catgatcaac tgggcgaaga gggtgccagg
    13501 ctttgtggat ttgaccctcc atgatcaggt ccaccttcta gaatgtgcct ggctagagat
    13561 cctgatgatt ggtctcgtct ggcgctccat ggagcaccca gtgaagctac tgtttgctcc
    13621 taacttgctc ttggacagga accagggaaa atgtgtagag ggcatggtgg agatcttcga
    13681 catgctgctg gctacatcat ctcggttccg catgatgaat ctgcagggag aggagtttgt
    13741 gtgcctcaaa tctattattt tgcttaattc tggagtgtac acatttctgt ccagcaccct
    13801 gaagtctctg gaagagaagg accatatcca ccgagtcctg gacaagatca cagacacttt
    13861 gatccacctg atggccaagg caggcctgac cctgcagcag cagcaccagc ggctggccca
    13921 gctcctcctc atcctctccc acatcaggca catgagtaac aaaggcatgg agcatctgta
    13981 cagcatgaag tgcaagaacg tggtgcccct ctatgacctg ctgctggagg cggcggacgc
    14041 ccaccgccta catgcgccca ctagccgtgg aggggcatcc gtggaggaga cggaccaaag
    14101 ccacttggcc actgcgggct ctacttcatc gcattccttg caaaagtatt acatcacggg
    14161 ggaggcagag ggtttccctg ccacagcttg atgaagatct gagctcttaa ttaatgatca
    14221 taatcagcca tatcacatct gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
    14281 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
    14341 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    14401 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgtt
    14461 taaactaact ttaaataatt ggcattattt aaagttacat atggcggccg ccctaaggct
    14521 cgacctcgaa attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
    14581 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
    14641 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
    14701 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
    14761 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
    14821 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
    14881 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
    14941 cgggcgcccg aaggtcttcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
    15001 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcatccat ctagatctcg
    15061 agcagctgaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
    15121 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
    15181 acaccgtcga tccagatcgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
    15241 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
    15301 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
    15361 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
    15421 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
    15481 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
    15541 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
    15601 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
    15661 gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac
    15721 gaccccatgc atcgatgata tcagatcccc gggatgcaga aattgatgat ctattaaaca
    15781 ataaagatgt ccactaaaat ggaagttttt cctgtcatac tttgttaaga agggtgagaa
    15841 cagagtacct acattttgaa tggaaggatt ggagctacgg gggtgggggt ggggtgggat
    15901 tagataaatg cntgctcttt actgaaggct ctttactatt gctttatgat aatgtttcat
    15961 agttggatat cataatttaa acaagcaaaa ccaaattaag ggccagctca ttcctcccac
    16021 tcatgatcta tagatctata gatctctcgt gggatcattg tttttctctt gattcccact
    16081 ttgtggttct aagtactgtg gtttccaaat gtgtcagttt catagcctga agaacgagat
    16141 cagcagcctc tgttccacat acacttcatt ctcagtattg ttttgccaag ttctaattcc
    16201 atcagaagct ggtcgagtaa ctttaaataa ttggcattat ttaaagttag gcgcgccata
    16261 acttcgtata gcatacatta tacgaagtta tgtcgagata tttaaatatt agacccagct
    16321 ttcttgtaca aagtggttga tatctctata gtcgcagtag gcgggggggg tctgaatcag
    16381 cccagggcat ctggggagca ggaggcaccg gcctgggaac agtggctgtt ggatgggttt
    16441 ggggaagctt gccctgcctc ctctccaccc tcatttccat ctttcctttc aggtcttggg
    16501 aattatcttc tcctgctgtc tggtgaagag tattcgaagt ggctatgaag taatgtaggg
    16561 gtggggggcg tttggtcttt tcatggagtg gattctccag gtttttcaat taaacggatt
    16621 attttttcag acctaaaaaa ggagatggtc tcagtttatc ttagagtgat ggatggcccc
    16681 ttccttttcc ttctccctgt cgctgccctg aaattaggca ggcatattta ggggcatttg
    16741 gggaaaggtg tattttcttg agggtgttgg ttgggctggc agtggaagag gtggggaatc
    16801 cagaggaggt tgtagctgag tgctctcctc tgcccctggc tttgtgctgc ctgcttagcc
    16861 ttcaaacagt gttgccgcag catccttgag gccatctcct gcataagtca aagggagttt
    16921 ccatagaggt cctacgcagt agagcctgga catgtctatt ttggacaact ttttggcttt
    16981 ctgatgcttt gagggccagc aaactcctcc ctcaaccctc aagaagaatg tttaagaatt
    17041 ttgccgcctg tgtggcagtg tccctgagtt tatttccttc tttcacggaa gccacccaat
    17101 ctggctatga tcacttttca tcactttccc ttctatcccc aggaaaacta gacaatggtg
    17161 tgccaggcgc caactaggaa ttctcatttc tctgctgaga aaagcgccct ctggtggcca
    17221 tctctccaag tgctgcacca aaaagcatct gtaggtgcaa ggtagctcct tacaccaccc
    17281 agtgtacaat agaagtttcg caggcactaa agaggcaaga gagtttgctc cctgggcagc
    17341 ttagggattt tagatactta aatcttgtca ggctcccaca ggtaccttca tttctgtaca
    17401 cctgcttttt gatttaaagc aatacatatt tattgagagc ctaattggta tcagaaacca
    17461 ggcagcactg ggtagacaca gaaatagatg ccttgctttt caaaatcagc ctcttgctgt
    17521 aaagctggaa tcaggagact gactgggcgg gccggggaag gatccaggtg agcacagcat
    17581 ccaccaccct ggctggaagg taggaggcag gcagccagag cagcttggca tcccagcctg
    17641 ggctgtagcg tgttcggggg tggcgagcag tcagggcatg ctccaggcag ctggtcacct
    17701 tcgttagttc tgggtcacag atcaggttca tgatgcggcg ctgtactcga agatctgtag
    17761 cttggaagat gctgtagtta gcgtgcatgc tctcactgcc gctcctccat cccgtgctag
    17821 atccttgggc tggttgccag gttcaggaag tgacaatatc ccctctgcct gcccttttct
    17881 tccctatgag gcctgacctt tctggccctc tctttttccc accatgctcc caatatgggc
    17941 ccagctactc acaagtatcg aggaaggctt ccccgtagtg ggcctgtata gctggaggta
    18001 gccgggccca acaagccttc agggtgctct ccagactctc caggttggtc acaggggttc
    18061 gaaagaagcc aggctccaca atggagactt gtactccgaa cggagccatg tcccgcctgg
    18121 agggtgggca gagaggttcc tgtgttttaa ctcaggtttc caggagtgtg gaatctaaga
    18181 tggaaaggtg tcacaggtag ggacatgtgt ccgacaggaa aggctattgc tggttggcct
    18241 tggatgggaa cacaggagtt ttttgttttt acttcctggt ggtgaggata aaacccaggg
    18301 cctgtgtgtg gtaggtaagc aactgagcta catcaatatc agcccccagc ccagggcgag
    18361 gtatcattgg gcatgttttt cttgcatttt ctttttaaag aagatttggt ggttatttat
    18421 atgtgtatgg ctgtatgagt tcccgtgtac catgtgtgca ggagccatca ttggtcagaa
    18481 gtcattactg gagttaaagg ctattgtgag ccaccttgta ggtgttggaa actaaacctg
    18541 ggccctctgc aagagcagta atcatttatc ctgagtcatc tctctggacc cttgtttttg
    18601 tttttttgag acagggtctc atgtagccca ggctagctct gagctaagta gccaaggata
    18661 actttgcact cttggtaact tctgtctcta cctcccatgt gttgagataa catgtgtctc
    18721 tcttctccct gagacagggt ttctctgcat agccctggct gtcctggaat tcactttgta
    18781 gaccaagctg gcctcaaact caagagatct gtcttcctct acctcccagg tgctgggatt
    18841 aaaggcatgt gccaccatgc ttggcttctc tattttaata ttttattttg tgagacaggg
    18901 tttcactatg tagttgaggc taatcttgaa attttctttt acttagaaaa attgtatttg
    18961 ctttatgtat gtgtgtgtgt gtgtgccact ggccatgtgt ggatgtcaga ggataacttg
    19021 gttctctcct accaagaatg tccaccaaat tcagggtgtc aggcttgcaa gcaagtgctc
    19081 ttaagtgctt agccattttg ttggttttca tgtagctatg tagtagaagc tggccttgaa
    19141 gggggtaaaa tacaggtgtg tgaccaccat gcttggagtg tatgtgtgag acaatatctt
    19201 gttgcttgga gtgtgtgaac atgcacgtgt ctgtgtgtga gacaaggtct tgttatgtag
    19261 cctaggctgg cctcaactcc ctgcttcagc ttttgtagtt ttgggattgc tggtgggtat
    19321 tactatgcgt atctctcctg tgtattctat tttgtgatct agactagtgg ctagtaagct
    19381 ttttgggatg gtggtgcaca ggatcaagcc tgagacctca tgctgcaaaa gtagcccaga
    19441 ttaacttaga attcaggatc cttctgcctt agcatcctgt tcccttactt cttcagaagg
    19501 attcctgtaa atgtgtacaa gtgtgagtgg ggtatgcaca tgtgcatgaa ggccacaaga
    19561 gggcatctca ttgcttgtga taatgagggg tcctcattat tacactgtga gaagtctccc
    19621 tacctacccc gccccctact ttttcttttc aaaagattta ttttatgtaa gtacactgta
    19681 gctgtcttca gacacaccag aagagggcat cagaccccat tacatatggt tgtgagccac
    19741 catgtggttg ccgggatttg aactcaggat ctctggaaaa gcagttaact gctgagccat
    19801 ctccccagct cctacttttt tttttttttt tttttggttt tttgagacag ggtttctctg
    19861 tatagccctg gctgtcctgg aactcacttt gtagaccagg ctggcctcga actcagaaat
    19921 ctgcctgcct ctgcctccta attgctggga ttaaaggcat gtgccaccac accaggctcc
    19981 tacttttttt taatagtaaa ttttgaggta ctacaagcta gacaaaacga acgaatgttt
    20041 ttattttgag atggggtttc accatgtaac tcc
//