LOCUS       42446#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20941 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 42446
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000047045
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            4438..4681
                     /db_xref="ENSEMBL:ENSMUSE00000421258"
                     /rank="2"
                     /note="ENSMUSE00000421258"
     exon            5441..5651
                     /db_xref="ENSEMBL:ENSMUSE00000233168"
                     /rank="3"
                     /note="ENSMUSE00000233168"
     misc_feature    4275..8924
                     /note="5 arm"
     misc_feature    8952..8976
                     /note="B1 Gateway"
     misc_feature    8989..9036
                     /note="Frt"
     misc_feature    9037..9536
                     /note="mouse En2 intron"
     primer          complement(9105..9126)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9001..9024)
                     /note="FRT3"
     primer          13202..13220
                     /note="seq primer NF"
     misc_feature    13262..13269
                     /note="RE sites"
     misc_feature    15062..15095
                     /note="loxP"
     misc_feature    complement(13270..13301)
                     /note="Rox"
     misc_feature    complement(15022..15053)
                     /note="Rox"
     misc_feature    13302..13323
                     /note="polylinker"
     CDS             13882..14478
                     /note="puromycin N-acetyl transferase"
     misc_feature    13997..14002
                     /note="Mutated RsrII site"
     intron          9537..9911
                     /note="mouse En2 intron"
     misc_feature    9909..9911
                     /note="Splice Acceptor"
     exon            9912..9965
                     /note="mouse En2 exon"
     primer          9812..9833
                     /note="seq primer FCHK2"
     misc_feature    9966..10088
                     /note="1Dleader"
     misc_feature    10089..10140
                     /note="F2A"
     misc_feature    10236..10952
                     /note="EGFP"
     misc_feature    10146..10235
                     /note="3X NLS"
     misc_feature    13018..13256
                     /note="SV40 polyadenylation site"
     misc_feature    12051..12995
                     /note="ERT2"
     promoter        13334..13845
                     /note="PGK promoter"
     misc_feature    14589..15021
                     /note="PGK pA"
     primer          13076..13099
                     /note="LRPCR RAF5"
     misc_feature    10962..11015
                     /note="T2A"
     misc_feature    11012..11012
                     /note="A to G to kill RsRII site"
     misc_feature    11016..12041
                     /note="Cre"
     misc_feature    9927..9966
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    9927..9942
                     /note="FCHK QC prefix region"
     misc_feature    9943..9960
                     /note="FCHK QC critical region"
     misc_feature    9961..9966
                     /note="FCHK QC suffix region"
     misc_feature    15118..15138
                     /note="B2 Gateway"
     misc_feature    8925..15168
                     /note="Synthetic Cassette"
     exon            15538..15745
                     /db_xref="ENSEMBL:ENSMUSE00000400031"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000400031"
     misc_feature    15169..15899
                     /note="Critical Region"
     misc_feature    15923..15956
                     /note="Downstream LoxP"
     misc_feature    15900..15979
                     /note="synthetic loxP region"
     exon            20229..20372
                     /db_xref="ENSEMBL:ENSMUSE00000412236"
                     /rank="5"
                     /note="ENSMUSE00000412236"
     misc_feature    15980..20941
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaattgtt agggctaagc tggtgacatg cagaattaag aaacattcag
     4321 tagtggggac actgatcagt ggctaatggt gacagaccta tgcgaggatg gagcgttctt
     4381 caggaatcca ctcccagtct tcagctaaca ctctcccctc ctctctcttc cctacaggca
     4441 tttcccactt gctaccagaa aataaagttt ttcaggaacc atgaaggttc tcctccactg
     4501 tcagagactt agattcatct ggcttgccaa gccagccggc cggcacttcc acagagattc
     4561 ccagctctgg gcacctctga cactggatga ctttgaagcc atcaatcgct gtgagaagcc
     4621 attacccaag aactttaact ttgcagcaga tgttctggac cagtggtccc taaaggagaa
     4681 ggtatcaaag agcagggccc cagtgttcct gttgctctca tctctgtctt ccaggtcctc
     4741 tcactgtgtt ctacctgtca agtttaaatc aattttttta tctcagtgag ggcaggaacc
     4801 tggatggctt catggagact cataagtaca agagcccata aacacctttc attccaatca
     4861 gtttctgtct tttgattctt ctcagaaaac ttagagtcta gggagatgaa ttcagattaa
     4921 ctcttccttg gtatatgctt atcccctctt taaaaagggc agaccccacc aagaggtgat
     4981 tctccaagac tgttctccag tgttccactg gagagaaaca tttctcccct aggcacactg
     5041 gggaagcagc tctggaggag tagcaataga cgtcctatct gccttctgtg ttagtcagtg
     5101 tgccatcact gtgacaaaac acctaagaca cacaatttca agtgaatgaa ggttgcttcg
     5161 gttcatgggt gcaacgatat cagcttcggt tcacttagcc ctgtggcagc cctgagcagc
     5221 acagggaaca tgcatggtga ggaagactgc ctgctcccct ccaggacagc tcagagaggt
     5281 ggaaggaatg agtgagagga agggcctagg gtctgatcgt tttcttcttt aagccctaac
     5341 gccacaggat aggggactat gccttttaac atgtgggtct tcaggtgaca gctctaaacc
     5401 ataatacatt atcttctctt tcccatgtgt tcctggccag tctggagaga ggccagccaa
     5461 cccagccctg tggtgggtga atgggaaagg agatgaggtg aagtggagct tccaagaact
     5521 gggttccttg tcccgaaagg ctgccaatgt gctcaccaag ccctgcggcc tgcagagagg
     5581 agaccgggtg gctgtgattc tgccccgtat ccctgaatgg tggctcatca atgtggcttg
     5641 catgagaaca ggtgagtatc acaaatgagt gtcaaaactt gagacttttg tgaagggtca
     5701 gctttgacta aagcaatcct tacaaaaagt agagaaagaa agttaacctt aatagcagtt
     5761 gtgatgaaat aataacaaca aacttaagaa attgggaaca ctgtgataac cattttttga
     5821 gagaggaaat caaagcacag tgatattaag acatttgcca aaggtcacat cacttgaaaa
     5881 ttatgtaatt ggggacctgg gaagatgact cagtggataa agtacttgct gtacaaacat
     5941 gtggccctgc attcccacat caaaagcttg gcatggaggg acaggtttct atgatcccag
     6001 cactgtgggc gcagagacag gcacatcccc tggtctcact ggccagccac acctagtcaa
     6061 gacagaaagt ctcaggttca cacacatata cccacacaca caaataaaaa tgaaaaagag
     6121 agttgcacta ttcagagttg cagaaaaaag ctctataaag caaatgcaag tgtgagtgtt
     6181 tctccacagc acagggtgtg tggaatatcc cccccataca cacacacaca cacacacaca
     6241 cacacacaca cacacacaca cgcgcgcaca cacgcgcgca cacacacaca cacgcacaca
     6301 cacggttctc tcttctgggt gtgtatctag attctctgca acacaaaaat acagaagaag
     6361 tggaagtctt tgaggatgca aagatttgta agaacataag ggactctgga cccaagtttg
     6421 gaaaaaaaaa aaaaactcca gtcagaagaa ctaatgtatc cagttacaca ttttccccaa
     6481 agtagtaaca tgtaaagtaa agacatgtct ttcagatagg ggtctgtgag ccatgaacta
     6541 tggttcctct gcttgggtaa atagacgtgt gatggaatac agctgactcc ttttgctcca
     6601 cagcaaacgt gctgactgag accaagaaca atcctacagg ccacagagtc taacacactc
     6661 tccaggcctt ttaccaaaag actctctgat ttcttatctt taaaaaaaaa aaatcatgcc
     6721 gggcatggtg gcacatgcct ttaattccag cactcaggag gcagaggcag tcagacttct
     6781 gagttcaagg ccagcctggt ctacaaagtg agttctagga cagccagggc tatacagaga
     6841 aaccctgcct cgaaaaacca acaaaaaatt aaaaaataaa aaaaatcatc agacatccat
     6901 taaaataaat aagaatgcca aagtgtagac tactacagag aacagaaaga tatctgtgca
     6961 atgttattag caggtataca aaaccaaact taaatagaaa tgattaaaca atgcataacc
     7021 tagctaaggt gtaagtttag ttgagttgtt gattgatggt tgaatggctt gatcttgatt
     7081 aaaaaataat ttatactagc aataataaca tcaggagaat ttcagagaat ttaagatgct
     7141 tttaatgcca ataaaataca tgaaagcagg agtataaaaa tgtttaaatt caccatataa
     7201 aaagacagag ataaattgga aatcagccgt ggttggaaga agattaagga tggtttatct
     7261 gttatgaaat atacccttaa tgtaatttaa agtaaattat ttaacttagg aaaatctgtg
     7321 agtacaaaca cacaagctag tgttcaaaaa catgaaataa aacaataaca acagttcatc
     7381 aagtgaagaa agccagctgc agacctgctt attccccaaa atgtcaaacc cttcatgtga
     7441 atttttaaaa ttgccttcac tcaccacata acaaactatt tgattttgct ggaaggcata
     7501 tgcaataaag tctaaaatac ttgccatcca acaaacattt gctgtctaaa acccattaga
     7561 agaaatcttc ctctgactcc caaggtggta atctactact gaacacattc ataaatccac
     7621 cacggagact gtaactggga atcattaaga gcaaataact tgtaagagaa atcattttat
     7681 atttttatat taattgatcg ttctgagact ctctaacgtg ttttcttaag ggaataactc
     7741 agctaactca gttaaaatat actcagtaaa atagccttgg gatggcaaga tggctctgca
     7801 ggtaaaggtg cttgctatgc aagtctggcc accagagctt ctgagtccat gagaccaacc
     7861 taaaggtgga aggagagcgt tgatattaca aaaggtgtcc tctgcccata tgcgtaccac
     7921 agcatgagag ctccccacaa taacactgat gacagtaaaa aaagagcctc cttcctagga
     7981 attcaactcc acaacttcct cctatttagc tgatttcaga atacccagca aggagtacat
     8041 acatagacat acaacacagg tcttgggagt gatagaaatg tctctgggga ctaaccttgc
     8101 tattggcatt gccatttatt ggctttgtga cccgtaggtg cgttatcatt cactggcatg
     8161 tgatatgtgc tctgtgcacg gcagtggcta tctgtaatgt tttgagtatc actgtcttcc
     8221 catggattca ctttagagag tcaaaccacc tgcttacatc ttatccttac atctaatcct
     8281 tacaccatac atgtaatcct gccctgtgca gtcttctcat aaggagcttt gaaatcctgt
     8341 ctctggctgc tgtttgggat gaagagggat aaattaagca tgaccatcgt catctaggaa
     8401 aatcttggcg gtaccagacc acggcatcaa tgatttgtag gctggctctg ccactcacca
     8461 gctatcccca aagactgcat gcacctcgtg gggatgctta aaacagtagt ttcaggttac
     8521 taaggtggct tggttactaa agatgcttgc tgcacgagcc tagctacatg agttagattc
     8581 ctaggaccca caaaaaggta caaggaaaga accacatcca caatggtatc atctgacctc
     8641 catgcaaaca acacacacac atacacacac acacacacac aaatgataga ttaaaaaaaa
     8701 tccttttaag tttttgaaga atagtctctg agcatctggg gctatgcaat ctatcaaaaa
     8761 aaattaatga gcaggcattt tcctagctgc atataatttt tcactctgtc ctgttgcact
     8821 gatgtagtat gcatcttaca aagcatttca acaaagagaa gaattctagt gtctctgtac
     8881 cttttacata tctattattt gcttttctca ttcccagata aattaaggcg cataacgata
     8941 ccacgatatc aacaagtttg tacaaaaaag caggctggcg ccggaaccga agttcctatt
     9001 ccgaagttcc tattctctag aaagtatagg aacttcgaac cctttcccac accaccctcc
     9061 acacttgccc caaacactgc caactatgta ggaggaaggg gttgggacta acagaagaac
     9121 ccgttgtggg gaagctgttg ggagggtcac tttatgttct tgcccaaggt cagttgggtg
     9181 gcctgcttct gatgaggtgg tcccaaggtc tggggtagaa ggtgagaggg acaggccacc
     9241 aaggtcagcc ccccccccct atcccatagg agccaggtcc ctctcctgga caggaagact
     9301 gaaggggaga tgccagagac tcagtgaagc ctggggtacc ctattggagt ccttcaagga
     9361 aacaaacttg gcctcaccag gcctcagcct tggctcctcc tgggaactct actgcccttg
     9421 ggatcccctt gtagttgtgg gttacatagg aagggggacg ggattcccct tgactggcta
     9481 gcctactctt ttcttcagtc ttctccatct cctctcacct gtctctcgac cctttcccta
     9541 ggatagactt ggaaaaagat aaggggagaa aacaaatgca aacgaggcca gaaagatttt
     9601 ggctgggcat tccttccgct agcttttatt gggatcccct agtttgtgat aggcctttta
     9661 gctacatctg ccaatccatc tcattttcac acacacacac accactttcc ttctggtcag
     9721 tgggcacatg tccagcctca agtttatatc accaccccca atgcccaaca cttgtatggc
     9781 cttgggcggg tcatcccccc ccccaccccc agtatctgca acctcaagct agcttgggtg
     9841 cgttggttgt ggataagtag ctagactcca gcaaccagta acctctgccc tttctcctcc
     9901 atgacaacca ggctcccagg tcccgaaaac caaagaagaa gaacgcagat cgcagatcgc
     9961 agatctctag agtcaccgag ttgctttacc ggatgaagag ggccgaaaca tactgtccaa
    10021 ggcccttgct ggcaatccac ccaactgaag ccagacacaa acagaaaatt gtggcaccgg
    10081 tgaaacagac tttgaatttt gaccttctca agttggcggg agacgtcgag tccaaccccg
    10141 gaccggtgca tgtggacccc aagaaaaagc ggaaggtgga ccctaaaaag aaacgcaaag
    10201 tggatcctaa gaaaaaaaga aaggtgcacg tgtccatggt gtccaagggc gaggaactgt
    10261 tcaccggcgt ggtgcccatc ctggtggaac tggatggcga cgtgaacggc cacaagttca
    10321 gcgtgtccgg cgagggcgaa ggcgacgcca catacggaaa gctgaccctg aagttcatct
    10381 gcaccaccgg caagctgccc gtgccttggc ctaccctcgt gaccacactg acctacggcg
    10441 tgcagtgctt cagcagatac cccgaccata tgaagcagca cgacttcttc aagagcgcca
    10501 tgcccgaggg ctacgtgcag gaaagaacca tcttctttaa ggacgacggc aactacaaga
    10561 ccagggccga agtgaagttc gagggcgaca ccctcgtgaa cagaatcgag ctgaagggca
    10621 tcgacttcaa agaggacggc aacatcctgg gccacaagct ggagtacaac tacaacagcc
    10681 acaacgtgta catcatggcc gacaagcaga aaaacggcat caaagtgaac ttcaagatcc
    10741 ggcacaacat cgaggacggc tccgtgcagc tggccgacca ctaccagcag aacaccccta
    10801 tcggcgacgg ccctgtgctg ctgcctgaca accactacct gagcacccag agcgccctga
    10861 gcaaggaccc caacgagaag agggaccaca tggtgctgct ggaattcgtg accgccgctg
    10921 gcatcaccct gggcatggac gagctgtaca agcctgcagg cgagggcaga ggaagtcttc
    10981 taacatgcgg tgacgtggag gagaatcccg ggccgtccaa tttactgacc gtacaccaaa
    11041 atttgcctgc attaccggtc gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca
    11101 tgttcaggga tcgccaggcg ttttctgagc atacctggaa aatgcttctg tccgtttgcc
    11161 ggtcgtgggc ggcatggtgc aagttgaata accggaaatg gtttcccgca gaacctgaag
    11221 atgttcgcga ttatcttcta tatcttcagg cgcgcggtct ggcagtaaaa actatccagc
    11281 aacatttggg ccagctaaac atgcttcatc gtcggtctgg gctgccacga ccaagtgaca
    11341 gcaatgctgt ttcactggtt atgcggcgga tccgaaaaga aaacgttgat gccggtgaac
    11401 gtgcaaaaca ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg
    11461 aaaatagcga ccgctgccag gatataagga atctggcatt tctggggatt gcttataaca
    11521 ccctgttacg tatagccgaa attgccagga tcagggttaa agatatctca cgtactgacg
    11581 gtgggagaat gttaatccat attggcagaa cgaaaacgct ggttagcacc gcaggtgtag
    11641 agaaggcact tagcctgggg gtaactaaac tggtcgagcg atggatttcc gtctctggtg
    11701 tagctgatga tccgaataac tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc
    11761 catctgccac cagccagcta tcaactcgcg ccctggaagg gatttttgaa gcaactcatc
    11821 gattgattta cggcgctaag gatgactctg gtcagagata cctggcctgg tctggacaca
    11881 gtgcccgtgt cggagccgcg cgagatatgg cccgcgctgg agtttcaata ccggagatca
    11941 tgcaagctgg tggctggacc aatgtaaata ttgtcatgaa ctatatccgt aacctggata
    12001 gtgaaacagg ggcaatggtg cgcctgctgg aagatggcga tctcgagcca tctgctggag
    12061 acatgagagc tgccaacctt tggccaagcc cgctcatgat caaacgctct aagaagaaca
    12121 gcctggcctt gtccctgacg gccgaccaga tggtcagtgc cttgttggat gctgagcccc
    12181 ccatactcta ttccgagtat gatcctacca gacccttcag tgaagcttcg atgatgggct
    12241 tactgaccaa cctggcagac agggagctgg ttcacatgat caactgggcg aagagggtgc
    12301 caggctttgt ggatttgacc ctccatgatc aggtccacct tctagaatgt gcctggctag
    12361 agatcctgat gattggtctc gtctggcgct ccatggagca cccagtgaag ctactgtttg
    12421 ctcctaactt gctcttggac aggaaccagg gaaaatgtgt agagggcatg gtggagatct
    12481 tcgacatgct gctggctaca tcatctcggt tccgcatgat gaatctgcag ggagaggagt
    12541 ttgtgtgcct caaatctatt attttgctta attctggagt gtacacattt ctgtccagca
    12601 ccctgaagtc tctggaagag aaggaccata tccaccgagt cctggacaag atcacagaca
    12661 ctttgatcca cctgatggcc aaggcaggcc tgaccctgca gcagcagcac cagcggctgg
    12721 cccagctcct cctcatcctc tcccacatca ggcacatgag taacaaaggc atggagcatc
    12781 tgtacagcat gaagtgcaag aacgtggtgc ccctctatga cctgctgctg gaggcggcgg
    12841 acgcccaccg cctacatgcg cccactagcc gtggaggggc atccgtggag gagacggacc
    12901 aaagccactt ggccactgcg ggctctactt catcgcattc cttgcaaaag tattacatca
    12961 cgggggaggc agagggtttc cctgccacag cttgatgaag atctgagctc ttaattaatg
    13021 atcataatca gccatatcac atctgtagag gttttacttg ctttaaaaaa cctcccacac
    13081 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
    13141 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    13201 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    13261 cgtttaaact aactttaaat aattggcatt atttaaagtt acatatggcg gccgccctaa
    13321 ggctcgacct cgaaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
    13381 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
    13441 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
    13501 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
    13561 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
    13621 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
    13681 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
    13741 ggggcgggcg cccgaaggtc ttccggaggc ccggcattct gcacgcttca aaagcgcacg
    13801 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcat ccatctagat
    13861 ctcgagcagc tgaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
    13921 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
    13981 cgccacaccg tcgatccaga tcgccacatc gagcgggtca ccgagctgca agaactcttc
    14041 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
    14101 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
    14161 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
    14221 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
    14281 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
    14341 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
    14401 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
    14461 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
    14521 gcacgacccc atgcatcgat gatatcagat ccccgggatg cagaaattga tgatctatta
    14581 aacaataaag atgtccacta aaatggaagt ttttcctgtc atactttgtt aagaagggtg
    14641 agaacagagt acctacattt tgaatggaag gattggagct acgggggtgg gggtggggtg
    14701 ggattagata aatgcntgct ctttactgaa ggctctttac tattgcttta tgataatgtt
    14761 tcatagttgg atatcataat ttaaacaagc aaaaccaaat taagggccag ctcattcctc
    14821 ccactcatga tctatagatc tatagatctc tcgtgggatc attgtttttc tcttgattcc
    14881 cactttgtgg ttctaagtac tgtggtttcc aaatgtgtca gtttcatagc ctgaagaacg
    14941 agatcagcag cctctgttcc acatacactt cattctcagt attgttttgc caagttctaa
    15001 ttccatcaga agctggtcga gtaactttaa ataattggca ttatttaaag ttaggcgcgc
    15061 cataacttcg tatagcatac attatacgaa gttatgtcga gatatttaaa tattagaccc
    15121 agctttcttg tacaaagtgg ttgatatctc tatagtcgca gtaggcggat taaatatcac
    15181 acaatcatta cacaatcacc tttcaagcct gacatgaaag tggttcttac tactcagccc
    15241 atcattgtca accagctgct gtcattatcc ccatgataaa agtaaagaag ggaaggccag
    15301 agaaacccaa gtcatagggc tagcaagttg caagaccaag actagaccca aggcctgtca
    15361 gattccaaag ccagagctct actgatccct catgccaggg cccagcagga cagcccaggg
    15421 aaccaagtta caggtagctg agggctgaca tctgaagttc tcattcatcc agacacaaga
    15481 cctagatttc ccaataatct ccagagctgt tagctcagga atcacttttt gttccagggc
    15541 ttgtattcat gccaggaacg atccaactga ccagaaaaga catcctctac cggctgcaag
    15601 catccaaggc caagtgcatt gtggctagtg aggaagtggc ccccgcagtg gactccattg
    15661 tatcagagtg tcccagcctg aagactaaac tcctggtgtc tccacaccac tgggatggat
    15721 ggctcaactt tcaggagtta cttcagtgag tatttgggtg ggaggcacta gcaacagcac
    15781 aaatcacagg tacagctctg aatctgtccg tccaaagact cagcaatagc cagagttgat
    15841 accgggctcc ttcccaaaac agaatgtttt taagagtatg agcttatgag tacatgtgtg
    15901 agatggcgca acgcaattaa tgataacttc gtatagcata cattatacga agttatggtc
    15961 tgagctcgcc atcagttcag aacatatgtc ctctgtaata ataataggta ctcttagacc
    16021 ctagaccatc tctccagctc cctaaaaagt catttaatga aattatttac agacagaaga
    16081 aatatgtaca gctctactct gacttttctg tctcctctgt ggcctatgat gagtgtgcta
    16141 tagagatggg cctttgggac acaactatca tgatctctct tttactcttt gaacatgctc
    16201 tagctcagaa ccttggcatc cactgtctct cctgccccga tgttctttcc tagtcctttc
    16261 tatagttgtt ctatcttgtc aggtttcagt tcaaatgttt tatcctacgt gatgctgctc
    16321 ctgaccatct tgatggaagc cgtaaggctt ttttctccat gcatggactg tttcttcgcg
    16381 ttgctaggtc ctctgagtga tcagactagc ctcgtgttct tttagattca gtttttctac
    16441 tccaaggttt ggactggaaa attaggctcc aaagccaaat cgggtccacc acttgctttt
    16501 gtaaataaag tgtcattggc acacagaggt gctcatttgt ttgtatattg tctatgtctg
    16561 ctttcattta gaaatggtat gcctgctatc tggccctttg cagaaagcat gacagccgct
    16621 gccttaagtt aaactctcta agtatgccaa gggttttctt gtgtgttcgg gtgatacctt
    16681 tatcttcaac acataacaaa acacagctgg gtgctgaagg aatagttaat aagcagacaa
    16741 aaaaaaaaaa aacagatcac ttaacctctg ttttcttcat atgtaaaatg gggataacta
    16801 tactgcaagt atctagtgac atttgacagc aaaataagct aatacatgag aaagaatttc
    16861 ccatgttatt ttaaaaagtg agaaaatgtt cattgacagc ataatcctta ttaaaatagc
    16921 agatgtttta taaaaataaa aaaaatccag cttatttttt ttgtaagtta taaactcagg
    16981 taagctatgt gacatttctg aaaatctgca tcttaatcta tgagatcagg ataatgacag
    17041 tatggggttg cagaaggaac aaacgggata aagcaatgtg acatgctcgg cataatggct
    17101 agaacaaatg gcacagtagt tggaactgtg tttgaggctg actcacatgg cagacaaagg
    17161 agcagttgag gcttggttga cttttggttg ggagataggt ggctgggtac tgtcattttg
    17221 gagaagggtg gatgggtact gtcatttgag gaaagatgag accagtgata cagcttgtca
    17281 gcacggtact tggtactgac tgaatgacaa gaccttggtc tccactctca gtgctacaaa
    17341 atgaaagcta aatgtagaca tggggtatat tcagaaggtc aaagcaggag aatgaagaac
    17401 ttagaagcca gtttaaggta cacaacaaga tactatctaa aaagaaggaa aataaaaata
    17461 aaacaggcag gcaggcagac aagaaggaag gaaggaaggg agggggacgg gtacggggat
    17521 ggagacggat gtagagcagg aaagaaccag cagagactgc tataagatag taagcttagt
    17581 tttaggggct gttaagagca ctgactattc ttccagagga cccaggtttg agtcccagca
    17641 cccacatggc agcccacaac catctgtaag ccaagttcta aatgttccaa tgcccaattc
    17701 tggccttcat gggcactgca tttatggggt acacagacat acatgcagga aaatcacaca
    17761 tttttaaaaa gacaataagc cagtcatatg gagatcaggg atctaggaag atccaggtgt
    17821 ctaacaagtg tggaatatgt gagtctcagg tataatgata aaaatcttga agttaccagg
    17881 aaaaagttat ctctgaaaat tcagaactca aaacttaggg agccaaaaag aaagaaaacc
    17941 cagacttagc atcaccccat gaatatcttt ctgcctctca gagactccaa tgtctttgtt
    18001 ttttcaggga aaagtaaaca catggatgaa gccttgtgtt agtaggtcct cttacttaat
    18061 ggagaaacac caaagacaag agatgtcccc taagctagga tgttagataa agtaaactaa
    18121 tcacactctc cggaacctaa aacatccaac atcacccaat atttgttctg aaagaaaaaa
    18181 aacaaaactc aatgatcttt tctattttaa ggaaacagag ttgctaaaaa tatctcaata
    18241 ttttgagaat ccatttttgg cattatggac tgaggatgtt tattacctaa tgatctctat
    18301 cttagtgcca tctcgatcaa tggcattcac tcatcattac aatcatctga atgttagaga
    18361 tgtacttcag atgatagaat ttgcctagca tgtgtgcagc tccagacttc tctagagcct
    18421 tgtaacttga atgtggtggt acaagcgtat aatctcagca cccaagaagt agcagcaaga
    18481 ggaagtttaa catcatcctc agatgcaatt ttcagccagt ctgggtctta tgagataatg
    18541 tctcaaaata aaatgataac ataaagttca ctgaaataaa aacctatcac ttggaatttt
    18601 ccaaaatgag aaatttgaaa aatataatac atgaggcata cagatagatg taatacagag
    18661 cacaatgatg cataccattc atcccagcat ccaggaggca aggcaagcag atatcatcaa
    18721 tgagtttaag gccagactgg tctacacgat gttccaggcc agtcatgatc tataatgtct
    18781 accagaagaa gtatgtcagt tcacctcatg tggccttctt tgtcacacaa gacaatctga
    18841 atcccagaat ccctgccaca aaatgtacac cagaataagt cataagcctc acccagatcc
    18901 ttcagtgtgg gaatggcatg tttgtccagg gatgggagga attggaaagc tgtgtacacc
    18961 aaaagtcttt ttctgtcacc taagatacac actttccacc ttagtaatcc aagtacagat
    19021 gaaacataca gtactgaggg gtgtctaggt ggttcgatga aacaccaacc ccatctttgt
    19081 tatgatgcca gtatgactag gagaattcca gacatgtaca ttgtatacat tctatacatg
    19141 gacaggtatc atgggttgtg atgaaagatg aatgggatgg gagacagaca atggcatcct
    19201 gtcttcatta ctcctttttt tcccatgaag gtgtgtgtgt gtgtgtgtat gtgtgtgtgt
    19261 gtgtgtgtgt gtgtgtgtgt gtgtgttgcc atgtgtgtag gtacatatat atgtaagagt
    19321 acacatgtac atgtttgcat gcaaaggcct gaggctgatg tcaggtgtct tcctcgatct
    19381 ctctgcctta ctcattgagt caggagctct cctcaaaccc agagcccatc aatgtcacta
    19441 gtcttgtgct agggaatcaa atctgacctt tcaagcaaag gaatcacgat gggccaccac
    19501 ggctccctgg catttacttg ggtgctggga tacaaacttt ggtcctcatc tacagcccac
    19561 atcactcttc ttaaaacaag aaaatgtctc ctgtaggact ttatacctgt tgactataat
    19621 tctcccaagc ctattcctaa accaatcact ggaaagggaa aaaaccatcc tgattggctt
    19681 agcttaattg tgactaagtg agaagcatgt ggctacctag tacctgaaca aaacctgact
    19741 tttgttagtt cagtaaaagg atgggatggc tatttggtcc gctattagct gtttctgtca
    19801 gagatactga aataatggac aataacaaca aaggttttaa gtttgatctc aatatatctg
    19861 gcctttaaat ctaaaggtct tgctctaatc cctaaatcat gaaatggtat caacttagaa
    19921 agactcaaat ctgtttttaa tttttttata taaattacaa agacattgaa ataaagtata
    19981 tgtgcttctg ttattgacat gacgagttac cttggctgtc tttttggact acaaagaaat
    20041 gagcttaaaa aggaggactt taccctgatc tttagagtcc ctctaagatt cttgggagtt
    20101 tctgctcagg gaagctctgc agtaggttca gttcattatt cccccgtggt aagtgggacc
    20161 ctagtagaga acaatcatac agagtcaact gatctcccct cagtctatat cttggggttc
    20221 atctgcagat cggcctctga agagcacaat tgtgtggaga cgggaagtca agaaccaatg
    20281 gccatttact tcaccagtgg gaccacaggc tcccccaaga tggctcagca ctcacagggc
    20341 agccttggca ttgggtatac cctttgtgga aggtaggaaa ggaaactatg gttttgttct
    20401 tattttcatt gcagcctggc ctcacactgt agttggctct ggactataac tcactctgta
    20461 cccttgactg gcctcaaatt catagcaatt ctcctgcctc tcttccctcc cccacaaaaa
    20521 tagttgaatt tgcaggagtg caccaccaca ctgcttcttt gttttctatg cagtctccca
    20581 ggctgccagt gtgggtgtcc aactgctgat atgacacctc tccttgaatg gatattaaga
    20641 tatctttaac gtagatccat aaaacagaac tctggatgcc tctaccctac cctagctaaa
    20701 tggtaccatt gaagtagctg atcaagcaag aatgtaggtt tagtgcttga ttcatagcac
    20761 ttctgcctct ctctctctcc ttatttccag gtgaatttac cttcccaaaa gggtctttga
    20821 aacaagtcta cctaaaactg ccacattgct ctgtttaatt ttccttctac ctaaagttaa
    20881 agcattcaat gattactctc aatcatcaac ccaataaagt ctagaatacc caagggattg
    20941 t
//