LOCUS       KO-first_condition_ready_34370_MGI:1346072        20934 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Psmf1 targeting project(s): 34370
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 51156
COMMENT     backbone: L3L4_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(125..378)
                     /note="BGH pA"
     CDS             complement(503..1159)
                     /note="DTA"
     promoter        complement(1173..1685)
                     /note="PGK promoter"
     promoter        1686..1836
                     /note="spectinomycin promoter"
     CDS             1837..2847
                     /note="SpecR"
     misc_feature    2995..3587
                     /note="pUC ori"
     misc_feature    3858..3885
                     /note="rrnB T2"
     misc_feature    4017..4060
                     /note="rrnB T1"
     misc_feature    4156..4176
                     /note="B3 Gateway"
     misc_feature    4182..4189
                     /note="AsiSI"
     misc_feature    1..4214
                     /note="Synthetic Backbone"
     exon            7551..7703
                     /db_xref="ENSEMBL:ENSMUSE00001260797"
                     /rank="2"
                     /note="ENSMUSE00001260797"
     misc_feature    4215..8408
                     /note="5 arm"
     misc_feature    8436..8460
                     /note="B1 Gateway"
     misc_feature    8473..8520
                     /note="Frt"
     intron          8521..9395
                     /note="mouse En2 intron"
     misc_feature    9393..9395
                     /note="Splice Acceptor"
     exon            9396..9568
                     /note="mouse En2 exon"
     misc_feature    9580..10173
                     /note="ECMV IRES"
     gene            10174..13234
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13273..13516
                     /note="SV40 polyadenylation site"
     misc_feature    13520..13553
                     /note="loxP"
     promoter        13580..14099
                     /note="human beta actin promoter"
     gene            14125..14919
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15119..15360
                     /note="SV40 polyadenylation site"
     misc_feature    15377..15424
                     /note="Frt"
     misc_feature    15431..15464
                     /note="loxP"
     misc_feature    9410..9487
                     /note="FCHK std_gpr"
     misc_feature    15480..15500
                     /note="B2 Gateway"
     misc_feature    8409..15530
                     /note="Synthetic Cassette"
     exon            15893..15975
                     /db_xref="ENSEMBL:ENSMUSE00000283692"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000283692"
     misc_feature    15531..16176
                     /note="Critical Region"
     misc_feature    16200..16233
                     /note="Downstream LoxP"
     misc_feature    16177..16256
                     /note="synthetic loxP region"
     exon            20581..20766
                     /db_xref="ENSEMBL:ENSMUSE00001312616"
                     /rank="4"
                     /note="ENSMUSE00001312616"
     misc_feature    16257..20934
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatatctg
       61 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      121 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      181 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      241 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      301 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      361 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      421 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      481 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      541 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      601 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      661 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      721 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      781 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      841 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      901 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
      961 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1021 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1081 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1141 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1201 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1261 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1321 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1381 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1441 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1501 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1561 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1621 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1681 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1741 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1801 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1921 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1981 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2041 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2101 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2161 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2221 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2281 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2341 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2401 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2461 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2521 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2581 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2641 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2701 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2761 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2821 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2881 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4021 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4201 aatttcacac aggaaacaga gtccagttag tgcttcctga cagaatattg cttattttgg
     4261 tgacttgctc ttacgaagat ggtattcatg ggtgctctgt gcatgtgtcc agaggggaga
     4321 gtttcataga attactcctc ctcctggttc tttactgcca atacacacac acacacatga
     4381 cgttttctct agaatactat aatttgatct agtgtgttcc ttctgactgc agtgtaatgt
     4441 gccactgtca gcatagtgtg cactttacct cggcttcagc tgttcaggct cacactggaa
     4501 tgaaggttga cattgcttat caggctccca catgtatcca cagcttcctt ctaggaggac
     4561 gtttgagtct tggctcatac aaacctcata gagctaagta ctgctaggtg gactcccaaa
     4621 gggcctgtgt aagtctgaat tcccagaagt agtgtcgaag gttcactcat gcccagtcaa
     4681 actaagtctt aggctggtgg ttgggtgtga ccttcattca tataatatat atatatatat
     4741 atatcttttc tggttttttt tttttttttt ttggtttttt gagacaaggt ttctctgtgt
     4801 tcccctggct gtcctggaac tcactctgta gaccaggctg gccttgaact cagaaatcca
     4861 cctgcctctg cctcccaagt gctgggatta aaggtgtgtg ccaccactac ccggctcatc
     4921 tcattatttt agctgccccc tgataactag taaatttgaa aaaacaaaca ccatttgtga
     4981 tggtttgtat attcttgtac cagggagtgg caccatttgg aggtgtggcc ttgttggaat
     5041 aggtgtgacc tggttggagt aggtgtatca ctgtgggtgt gggctcaata ctctcaccct
     5101 aggtgcctgg aagtcaatct tccactagta gcctttggat gaagatgtag aactctcagc
     5161 tctgcctgtg ccatgcctgc ctagatgctg ccatgctccc accttgatga taatggactg
     5221 acctctgaac ctgtaagaca gccccaattt aatgttgttt ttataagact tgccttggtc
     5281 atggtgtctg ttcacagtaa aaccctaact aagacaccat ttatacggtt agctgctctt
     5341 tttatttgtt ttccaaacct tgccttggtc tggttattaa ttcttgtcat ttgttccttt
     5401 tggctatagt gtctgtgcct ggacagaagc tctagggtat gggtggcctc acaagtggca
     5461 ttgtgtttgc tttagtggtc ctgcttaaga actttatttc ctacctgaaa ggccctgctt
     5521 gttttcacta tgacttgaga ttattctcat attttaatgt gtgaaaggga atgtcctggt
     5581 tgcttgagtt tgtttgtttg ttttaattaa agggttgggg ggagggactt tgaacacaaa
     5641 ctagagttat atgggaagag gaatctcatt tgagaaagtg cctccatgca cttaagaatg
     5701 acagtaagat aggtaagtct gtaggccatt ttcttgacga atgattaatg agggagagat
     5761 ggcccagctc actgtttagt tggtcctggg gtatatatga aatcaacctg agcaagccag
     5821 caagcagtgt acttccatag cttttgcttc agttcctgcc cccaggttcc tgccttgact
     5881 tccttctatg atatattgtg gtgtagaaaa ataaatagtg aggtaaataa aaataaaccc
     5941 tttcctcccc aagttgcttt tgattatggt attttatcac agcaatagaa gtcataatta
     6001 agacagggaa atcttagaat tattaaatag ggaaaggaag gagattatca caatgaatac
     6061 acagatatat tgtacagtct accagattgg caaaaacaaa caaacaggta atggcagcct
     6121 gcaggccatg tgtagaacca ggagctcaca tattgcaagt gtatcacgat ttggtacaca
     6181 gattgcttct attctatgaa agttcatcct ctgagcattc cacatgtgtg ccagatatgt
     6241 acaacaacat agcagcattg tatataatgg ggaaaaaaaa tcaacttcta atgaacttct
     6301 aaatcgtagc acattcattt ggtgcaacac tgtatagttg tgtatgtaga tcagccacag
     6361 aatgctgatc accttcccaa aacaacactg gatagaaggg aaagggaaag ggtggggcca
     6421 agaagatggc tcagagggtg aagtgccttc cctgtcttaa ttacatgagt acctgcgttt
     6481 ccattcctag ccaggagtag tgttgagtag aggaagaggc acttccctgg agctcactgg
     6541 ctagcctgct gagtcattga gctctaggtt cagtgagaga ctatgtttca gaaaataagg
     6601 tggacagcaa ctgagttcaa aagcattggg acggatggat ggatggatag atagatagct
     6661 tatattatat ttgaatatat atttagttta tttaatattt atgggtgttt tatcagcatg
     6721 tatgtctgtg catcttgtct atctggcacc cacttgggcc agaagaagat gccagatctc
     6781 ttaggactgg gtgatggttg aaagcctccg tgtgggtact aggagttaga tgggttcctc
     6841 tagaagagta gctggctgag ctgcatgtgt agtgatgtaa tgtgaaggaa aagtaaaaat
     6901 gatacaagta caaaagtaaa cacacacaca cacatacaca cggaatattg gttgtgcaca
     6961 ggctttagtg ataatgctta ctgactgatg acactgaact tgctatgcta gtgatgacta
     7021 cataagtatc tgttttacaa cttttaaatc ttgtttgtgt gttagatttg gggcatacag
     7081 taatgatttt tatcggttgg agagtctgtt aggatctgtg ctttcagtca ttttgtgatg
     7141 ctattacaga gaacccaagg tgtgaactgt ggaaaatact gcttttaatt tcacactgat
     7201 cttattcttg ttaggatgat agagtagaga gatgattatt aagccaaggc agtagttatt
     7261 ttaatttttg ttggttctgg gaattaggtc ctcatacatg ctaggcagca ctctactact
     7321 tagctctatt cctagcccgt agccttagct tcttgacaga ttcctccagt gttgtcctca
     7381 aggcaggcaa atagctgaat aagagtgtta gttgtagacc tctaactagg tgcgtatccc
     7441 tgcacacgtg tgatgggagg gatgagatca agttatgggt aatatggtcc ttcatagttt
     7501 tgggttctga ggccaagatc ttcctgtttt taagcctggt atcttctcag ccaggtccca
     7561 gtgataagaa atcagaacta ctgccagcta agtggaacag caataaagaa ctgtatgccc
     7621 tccggtatga gtctaaggat ggagccagaa aactccttct gaaagctgtc tctgtggaga
     7681 acggcatgat catcaacgtg ctggtgagtc tgagccaggg ttctgctaat ggtgtgggat
     7741 cgagatacct gtttagaaac tcattttaac agtgaactca gaaatgaccc caaagttttt
     7801 acctagcaat tccactttgg gcatctttct cctgaggtaa aaacccacaa tatatgtaga
     7861 agagttttgt gcacaaaggt gttcattagg aaccccctgt ccacagcagt actcactctc
     7921 tgacaacaca ggagataaag gcttctgtag ttgcagcctc tctcttagct tcgtaagtaa
     7981 gaggtttcac tgccttggac cctgtacatt ttgtttcttg ctctggagac taccacctcc
     8041 atcacatcac acacacacac acacacacac acacacacac acacacacac acacacatat
     8101 atatatacct tctgccatca ttcctttagc ctgcccccct cccccggtgt gtgtgtgtgt
     8161 gtgtgtgtgt gccctgacta tccaccccac ctgccagaca agtctaaatg atagctttct
     8221 attctatttt ccatctggta cataccatgt ttggtattta tgtacttatc tgtgcttgta
     8281 ttttcaccct ggactgtgaa caccttgagg tagatgactt tctttctgtt ccattttgtc
     8341 ttggttgcat gtgcacaaaa gtgtctgact gtattatttg tacttgactt tttccatacc
     8401 acgtgtttaa ggcgcataac gataccacga tatcaacaag tttgtacaaa aaagcaggct
     8461 ggcgccggaa ccgaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
     8521 gaaccctttc ccacaccacc ctccacactt gccccaaaca ctgccaacta tgtaggagga
     8581 aggggttggg actaacagaa gaacccgttg tggggaagct gttgggaggg tcactttatg
     8641 ttcttgccca aggtcagttg ggtggcctgc ttctgatgag gtggtcccaa ggtctggggt
     8701 agaaggtgag agggacaggc caccaaggtc agcccccccc ccctatccca taggagccag
     8761 gtccctctcc tggacaggaa gactgaaggg gagatgccag agactcagtg aagcctgggg
     8821 taccctattg gagtccttca aggaaacaaa cttggcctca ccaggcctca gccttggctc
     8881 ctcctgggaa ctctactgcc cttgggatcc ccttgtagtt gtgggttaca taggaagggg
     8941 gacgggattc cccttgactg gctagcctac tcttttcttc agtcttctcc atctcctctc
     9001 acctgtctct cgaccctttc cctaggatag acttggaaaa agataagggg agaaaacaaa
     9061 tgcaaacgag gccagaaaga ttttggctgg gcattccttc cgctagcttt tattgggatc
     9121 ccctagtttg tgataggcct tttagctaca tctgccaatc catctcattt tcacacacac
     9181 acacaccact ttccttctgg tcagtgggca catgtccagc ctcaagttta tatcaccacc
     9241 cccaatgccc aacacttgta tggccttggg cgggtcatcc ccccccccac ccccagtatc
     9301 tgcaacctca agctagcttg ggtgcgttgg ttgtggataa gtagctagac tccagcaacc
     9361 agtaacctct gccctttctc ctccatgaca accaggtccc aggtcccgaa aaccaaagaa
     9421 gaagaaccct aacaaagagg acaagcggcc tcgcacagcc ttcactgctg agcagctcca
     9481 gaggctcaag gctgagtttc agaccaacag gtacctgaca gagcagcggc gccagagtct
     9541 ggcacaggag ctcggtaccc ggaagatctg gactctagag aattccgccc ctctccctcc
     9601 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     9661 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     9721 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     9781 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     9841 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     9901 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     9961 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
    10021 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
    10081 tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
    10141 tttgaaaaac acgatgataa gcttgccaca accatggaag atcccgtcgt tttacaacgt
    10201 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    10261 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    10321 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
    10381 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac
    10441 ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt
    10501 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg
    10561 ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg
    10621 tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg
    10681 agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac
    10741 ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg
    10801 ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat
    10861 gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac
    10921 ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct
    10981 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg
    11041 aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt
    11101 gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc
    11161 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc
    11221 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg
    11281 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg
    11341 aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc
    11401 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta
    11461 ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg
    11521 atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc
    11581 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc
    11641 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg
    11701 gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg
    11761 atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg
    11821 caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag
    11881 tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc
    11941 gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg
    12001 catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg
    12061 caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac
    12121 tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct
    12181 ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa
    12241 ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac
    12301 atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg
    12361 tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat
    12421 aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa
    12481 aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt
    12541 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg
    12601 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg
    12661 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg
    12721 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg
    12781 agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag
    12841 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc
    12901 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc
    12961 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc
    13021 gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc
    13081 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt
    13141 ggtggcgacg actcctggag cccgtcagta tcggcggaat tccagctgag cgccggtcgc
    13201 taccattacc agttggtctg gtgtcaaaaa taataataac cgggcagggg ggatctaagc
    13261 tctagataag taatgatcat aatcagccat atcacatctg tagaggtttt acttgcttta
    13321 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
    13381 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    13441 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    13501 tatcatgtct ggatccggaa taacttcgta tagcatacat tatacgaagt tatgtttaaa
    13561 cggcgcgccc cggaattcgc cttctgcagg agcgtacaga acccagggcc ctggcacccg
    13621 tgcagaccct ggcccacccc acctgggcgc tcagtgccca agagatgtcc acacctagga
    13681 tgtcccgcgg tgggtggggg gcccgagaga cgggcaggcc gggggcaggc ctggccatgc
    13741 ggggccgaac cgggcactgc ccagcgtggg cgcgggggcc acggcgcgcg cccccagccc
    13801 ccgggcccag caccccaagg cggccaacgc caaaactctc cctcctcctc ttcctcaatc
    13861 tcgctctcgc tctttttttt tttcgcaaaa ggaggggaga gggggtaaaa aaatgctgca
    13921 ctgtgcggcg aagccggtga gtgagcggcg cggggccaat cagcgtgcgc cgttccgaaa
    13981 gttgcctttt atggctcgag cggccgcggc ggcgccctat aaaacccagc ggcgcgacgc
    14041 gccaccaccg ccgagaccgc gtccgccccg cgagcacaga gcctcgcctt tgccgatcct
    14101 ctagagtcga gatccgccgc caccatgatt gaacaagatg gattgcacgc aggttctccg
    14161 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
    14221 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
    14281 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
    14341 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
    14401 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
    14461 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
    14521 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
    14581 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
    14641 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
    14701 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
    14761 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
    14821 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
    14881 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
    14941 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
    15001 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
    15061 gggatctcat gctggagttc ttcgcccacc ccccggatct aagctctaga taagtaatga
    15121 tcataatcag ccatatcaca tctgtagagg ttttacttgc tttaaaaaac ctcccacacc
    15181 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
    15241 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    15301 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
    15361 gggggtaccg cgtcgagaag ttcctattcc gaagttccta ttctctagaa agtataggaa
    15421 cttcgtcgag ataacttcgt atagcataca ttatacgaag ttatgtcgag atatctagac
    15481 ccagctttct tgtacaaagt ggttgatatc tctatagtcg cagtaggcgg aagaactggg
    15541 ttcttaggtg caaacaattc ctttgggtta atttaaacat taaaagaatg gagttagttc
    15601 attaaagtag gttcaaatct aggcttccaa aagcagtacc ccaaacccta gaaaccactg
    15661 ttgcttggcc cctcaggctg ggtgtgggtt ggtattcagt ctgcagtgct ccatgctgtg
    15721 tgctgctccc ttcctgagca agatgcctgc ccttactggg cctgtgtttc tatgttgctc
    15781 caggttatgg ttgtgtattc acggggtact tcagttcctt gcccaccttc ccctgttacc
    15841 agtaacctgg gaaatatacc ccatctctca ttttctgttt tcacttttct aggaacttgg
    15901 cacacagcag gtggcagact tgaccctgaa cttagatgat tatattgatg cagaagacct
    15961 gagtgacttc cacaggtact tctaaaggaa gtctttccat gagaaaaggc agagggactc
    16021 agtagcaaga aataaggact ggtatcaaag tccccagaga caagtacggt tgacagaaga
    16081 acttgcccta ggcgcagtct gttctcagtt ccctccttct tacatatctg tttgtttgaa
    16141 gacaagactc acatagaaat gtctcattaa gtaaaagaga tggcgcaacg caattaatga
    16201 taacttcgta tagcatacat tatacgaagt tatggtctga gctcgccatc agttcaagag
    16261 gctaccgtgg ctatagtctt ggggcgaggc taatgcttca gtcaacagta agatccctgg
    16321 ggccagcaag tggctcagtg ggtaaagatg cttgctggta gaggtgcttg tgcacagaca
    16381 tgacaacctg ggttctgtcc ctgcgtccca tagtcagcag gagagaacca tgactgcaga
    16441 tttgttttct gatcacacat gtgccacgtg tgtgacactt taaaaaaaga ataattcctt
    16501 attgaaagta tgcttaaatc aagtacatct tcatttcact cccagctttt ttttttttta
    16561 agagtcaaaa tttagatccc atctgctcac cgttcccact cttgtgtgac accagagagg
    16621 ttcctcagtc ataagccata tacctacacc cacatcacct ccattttggg gtcactctta
    16681 gtgttgtgtg gtatgcgagg ttggacatac acacagtggc atatgcagtg tttcagtgtc
    16741 ataggccatt gtgttacact gtaagcatcc ttgtgttctt cccctcaccc ccaccccctg
    16801 tccttggaag cattggtcct gctgttgtct ccatagtttt acctcttcag catgtcatgg
    16861 ttggaaagga gtccagacaa actgtcttcc aaagcggctg tgctgctctg cattcctgct
    16921 gtcagggaat aaggagagct gctgctgccc agcccgtgcc ctgtcagcat tcagcagtgt
    16981 aatggtggct ttgcattgtg ttaagtgagg cagggtgtga gtcgtctttc atgggcttat
    17041 ttccctgtgt agttctttga ggaggctgtt tagatccttt gctcagtttc aacttcgctt
    17101 gttccctgtc tcactcggtg ttaagagttg gttgtgtagt ttgggtggca gtcctcattg
    17161 gccgcttctc catgtgttct ctccctgttt gtggagacat tccgctgact tcctaatttt
    17221 gaaatttgaa gagagacagt ttgcaaaaat acacagtaga tacccattta tctattaccg
    17281 agattagcca atttttaaca gttgttttat attcttgaac catctgaggg ttatttactt
    17341 tgttctttaa attctgcagc atggaacaac tgtgtgtgtg tatgtgtgtg tgtgtgtgtg
    17401 tggtgtgtgt gtgtgtgtgt gtgtgtgtgt gggcggggga gggagagaga gagggggtag
    17461 gaggacagcc tcaggagtaa gtcctcattt ataacagggt ctctctgttg ttttgcacgg
    17521 tgtacacaaa gctagctggc ctacgagctt caaggattcc tatttttgta tcctctttgg
    17581 ctgtatggtc actagaattg cagtgcatgt tactgggcct ggctttatag gtcctagcgt
    17641 cctcacactt agtgacaagt gctttatcct tcttccaaca cctccacctt agactagcaa
    17701 tttcatagga atatcatctg tctcaacttt gaacaatgat cacacttgag aatgtagcat
    17761 cagtatatca tttaatatgc agtctacatt ctgatttctg cacctatccc aacagtgttc
    17821 ttcagagcgg atcttttagg tttttttctt aatccaccta aatatttcct attacattaa
    17881 tttttatgtc tttttaatta attttaacta gcacagtttc tagacatttt tttcattttc
    17941 ttgttttttc aaaacttgaa gtaaatgtgt gtgtctgtgt gtgtctgcgt ctgtgcctgt
    18001 agggaccttg ctaagtaggt caggatcctt ggacctccat ggcaatttgt tctccatttc
    18061 taaatttttt cacttgaaac attttatata aatagaatta atatgtagtc ttgggcttgg
    18121 catttttact agcataatac tcttgtgatg gatccatcta aagtgtagtg cactttaact
    18181 gtgcctcttg tttactgagt atactttatg ttgcgtgtac aacagttgat ttgatcactc
    18241 actggtcaaa gggtaagtta attgactaga ttgtgtctag ttgggcaatc acagatatgg
    18301 atgccatagc atttgagcac aggctgtatg cagctgtgct ttttattctc tatgggggac
    18361 cccagagtgt ggtggtaagg tcatatttgg tttacttttg gtgttttctg aatcatttat
    18421 atccactcat tatgagtgca cccacttctc tgcatcttgt cctagcattt tattttatat
    18481 tatttgttga atgtgaagtt gtctctcatt gggatcatta atttgcattt ccctactagt
    18541 gaatgaaatt ttgaggtgtt ttgttttgtt gaggcaggat ttccttggaa atttgtcata
    18601 ggcaaaatgt ttcattcctc accttttaat tgctgtgatt ttcttctttt cccttttcac
    18661 agtgcctgta gctgccagtg ttgttaaatt agaattgcca cagagtttcc ttgtcatgtt
    18721 cccacagtta ggggtagagg atgcagtttc catcactgat tgtattgtta actatgggca
    18781 tgtggcagat gctctgtagt aagctgagga tggtctccta tatccttagt tcctttgtta
    18841 gtgctatttt tttttaccat gagtgcattt atcatgttgt tttgtgcttt atcagttggt
    18901 ctaattatct gatgtttatt tacttgttag aatagtggcc agcattgatt tgtgaatatt
    18961 aaaccagcct tgcttaccta gaataaattg tttaaggtca attttaatat ttttgaaagg
    19021 tttttgtttt gagttggtaa agaaattctg gtctgtaatt ttatttgttt agttgtctag
    19081 ttttgatact aactagcact ggcttcctag ttgtctagtt ttgatactaa ctagcactgg
    19141 cttcctagtt gtctagcttt gatactaact agcactggct tcctagttgt ctagctttga
    19201 tactaactag cactggcttc ctagttgtct agttttgata ctaactagca ctggcttcct
    19261 agttgtctag ctttgatact aactagcact ggcttcctag ttgtctagct ttgatactaa
    19321 ctagcactgg cttcctagtt atctagtttt gatactaact agcactggct tcctagttgt
    19381 ctagctttga tactaactaa ctagcactgg cttcctagca ctgcttggaa atgctcccac
    19441 ctgggcttct gaaaatgact tttttaacag tgaaagtaaa attttgtgac tagactttct
    19501 tgtattgtat ttatgtcttg agacgtgtct ttggaggcag gctatgtatt agttactttt
    19561 taaaatacca tgactaagta acttatagaa gagtttattt gggcttattg ttccagagga
    19621 ctaggggtcc atcatggaca ggaggcatgg ctacaagtgc aggctggagc aggatgctga
    19681 gggcctacat actgaaccac aagcaggaat ccaagagaat aaactgagaa tagtgcatgg
    19741 cttttgaaac cttgtcaggt gacacacttc ctcctgcagg accacatctc ctaaacctac
    19801 tccaatactg ctaccaactg gggaccaagt attcaaatgc caagaccatg ggggaacatt
    19861 tcattcagaa cactacagtg tgggttctgc cttttcctct ttgatccttg cattctttgg
    19921 aaatgattga gttccacaag ttcagaggcg tttgttttga gctctgatgt ggttccatat
    19981 ggccagggaa cacactctgt aggacttcag gtacggagtt tgctttatgg accaggactt
    20041 ggctcatttt taaggagccg ttctgcccac cctctgctgc ctgacctggc caccacacct
    20101 cagctgtagc tcatggtgct aatttctctt ggtgagaaga gacacctggt agagacattc
    20161 tcccagaatc tggctaaact gagcctcagg ccctacctct gtggttcatc ctcccacaga
    20221 gccttttcct tccatgacat gtgatgaaca cataatgaag ctgagggagg ggtggcatgg
    20281 gaacagaaga gcagccagtc caggctgtgc ccctactctc atctctttct gatggggtgg
    20341 taaatgagga ggggcaactc agatgcctaa gaaacttagg cccaaatctc ggagacagca
    20401 tcaggctgtt gcccccctca cccaggtccg ccggacggcc cgttcccacc atagtaaaca
    20461 ggccaagcgc aagaccaagg ctgctcaggg ccgccgccgc cgccgaccga gccatcagaa
    20521 tactgtacat gagactccca gccaactgca ggaagctaat ttcttgtctc aatcctgcag
    20581 gacctataag aacagtgagg agcttcggtc tcagatcaga tctggaatta tcacacctat
    20641 ccatgagcag tgggagaagg ctcgtgcaaa cagtcctccg agggagttcc cacctgccac
    20701 tgccagagag gtggacccac tccagatttc ctcacaccgt cctcatacta gcaggcagcc
    20761 tgcttggtaa gtatggagtc cccaaagttc agcccatcct gctgttgccc ctcccctggt
    20821 ctccaagtgt ctccagcaga cgtgttgaaa gtattggtgt acagaggtaa aaaagtagaa
    20881 atggtgatag atttgagttc tggtgctact gatagttact ctgactacag catt
//