LOCUS       232700#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        22335 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 232700
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000018713
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            9314..10067
                     /db_xref="ENSEMBL:ENSMUSE00000111616"
                     /rank="1"
                     /note="ENSMUSE00000111616"
     misc_feature    4275..10564
                     /note="5 arm"
     misc_feature    10592..10616
                     /note="B1 Gateway"
     misc_feature    10629..10676
                     /note="Frt"
     misc_feature    10677..11176
                     /note="mouse En2 intron"
     primer          complement(10745..10766)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10641..10664)
                     /note="FRT3"
     primer          14842..14860
                     /note="seq primer NF"
     misc_feature    14902..14909
                     /note="RE sites"
     misc_feature    16702..16735
                     /note="loxP"
     misc_feature    complement(14910..14941)
                     /note="Rox"
     misc_feature    complement(16662..16693)
                     /note="Rox"
     misc_feature    14942..14963
                     /note="polylinker"
     CDS             15522..16118
                     /note="puromycin N-acetyl transferase"
     misc_feature    15637..15642
                     /note="Mutated RsrII site"
     intron          11177..11551
                     /note="mouse En2 intron"
     misc_feature    11549..11551
                     /note="Splice Acceptor"
     exon            11552..11605
                     /note="mouse En2 exon"
     primer          11452..11473
                     /note="seq primer FCHK2"
     misc_feature    11606..11728
                     /note="1Dleader"
     misc_feature    11729..11780
                     /note="F2A"
     misc_feature    11876..12592
                     /note="EGFP"
     misc_feature    11786..11875
                     /note="3X NLS"
     misc_feature    14658..14896
                     /note="SV40 polyadenylation site"
     misc_feature    13691..14635
                     /note="ERT2"
     promoter        14974..15485
                     /note="PGK promoter"
     misc_feature    16229..16661
                     /note="PGK pA"
     primer          14716..14739
                     /note="LRPCR RAF5"
     misc_feature    12602..12655
                     /note="T2A"
     misc_feature    12652..12652
                     /note="A to G to kill RsRII site"
     misc_feature    12656..13681
                     /note="Cre"
     misc_feature    11567..11606
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    11567..11582
                     /note="FCHK QC prefix region"
     misc_feature    11583..11600
                     /note="FCHK QC critical region"
     misc_feature    11601..11606
                     /note="FCHK QC suffix region"
     misc_feature    16758..16778
                     /note="B2 Gateway"
     misc_feature    10565..16808
                     /note="Synthetic Cassette"
     exon            17105..17269
                     /db_xref="ENSEMBL:ENSMUSE00001250917"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00001250917"
     exon            17382..17466
                     /db_xref="ENSEMBL:ENSMUSE00001035976"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 2 ENSMUSE00001035976"
     exon            17717..18047
                     /db_xref="ENSEMBL:ENSMUSE00000111613"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 3 ENSMUSE00000111613"
     misc_feature    16809..18159
                     /note="Critical Region"
     misc_feature    18183..18216
                     /note="Downstream LoxP"
     misc_feature    18160..18239
                     /note="synthetic loxP region"
     misc_feature    18240..22335
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaaaatgt cctggggaag acatttaaaa ttgataccgg ttaagaacca
     4321 ctaccttgcc gggcgtggtg gcgcctgcct ttaatcctag cactcgggag gcagaggcag
     4381 gcggatctga gttcgagacc agcctggtct acaaagtgag ttccagggca gccagggcta
     4441 cacagagaaa ccctgtctcg taaaaccaaa aaaaaaaaaa aaaaaaagaa ccactgcctt
     4501 agtgtttgtt accagttagc accactgtgg gtgtctgtct gtctgtctct agtaaagagg
     4561 ggaaagcaag aacccaggaa ttcaaatagt tgggtagctg gactgtctca ccattggagg
     4621 ttttccgaaa agcaggagga ggggtctgga gaagcgactc aggggttaag agcgtcctcg
     4681 cccctttgga ggatctgaat tagaagccta gcactcacaa ctgtcagtaa tcccagtccc
     4741 agggatatga cgccctcttc tggcctctca gggcactgca tgcatgatgt acacagacac
     4801 acatacaagg acaaataccc aaacacataa aatgaaaata tgtaataaca acagcaacaa
     4861 caacaaaaca ggactcaaat ggatagatag atagttggac tgtaccatag ctagtgcctc
     4921 tgcctcccaa gattaaagtc taatggcctg agccaccact cctagctttg tgtcatactg
     4981 aggtgtggca gaggggctgg tgacatgtaa tctctactaa catcagtaga tctcaggata
     5041 gtgttaaata cactgtacga cccacagcag caaagacttt ctagctccaa gtgtcagcat
     5101 tgctgacatt gctccttttt gcctggtggt tgagtctgta atcccagcac cagaaagaca
     5161 agaggcaaga ggaagccagg actgtcttac tctccacagt gaggcagatc ctgtctcaaa
     5221 acctctacca aaaaagccag gcacggtgct ccatgcctgt aattccagca cttggggaaa
     5281 caaagtaagg agggtcatga agtccaggcc acccttgccc atgaaacaag accttgtctc
     5341 aaaaacaaaa caaaacaaaa caaaacaaaa caaaacaaaa caaaacaaaa caaaacaaaa
     5401 caaatgtaat cctgcttgga atcaatgact aagttccaag gggcaagtca gcaaagggcc
     5461 tttgctctcg ctcctctttt gcttatagca tggctctctc atgactgact cattatttaa
     5521 cagtcccttg ctcagaaagt cgccctgatt tcacttgaga ttggacccag ccttttgcat
     5581 taccctgctt cattaccttc aaaacaacca gtgttttatg tctttattgc ccttcttcct
     5641 ccttccagaa gctgccccag atgagcaccg tgtccttacc tgtcttgtcc cgaggcgtat
     5701 ccctagggtt aattaatccc aggacatttc taggtattca ggaaaccctt gtgggccagc
     5761 acatggacag catttctttt ttccagaatt caaaatctag gcacctctgc actttacaga
     5821 gcagcaggga aattgatact cagtccataa gttacacaaa gaacatttca gcctttgaga
     5881 agagccagga gaggaaagta caggtaacca cgagagccgt gagatgacct gactcagcgt
     5941 gggtggggca gccaggacgg acttcctcaa agagtcacaa aggatgcatt tctcggctgg
     6001 gtagagggag gtgggtgggg tgaatggatt agagcaccta agcatagagg acagaaagga
     6061 aggtgctttg gtgtggtgga acatgtactg tgggttttgg ggacagttga gggacaaggc
     6121 aggcatgtca gtcagtagtc cagagtgaca cttaggtgta gctcggtggc accagtactt
     6181 gtgtgaggct cttgagttca atccacagca acagaggaga agaaaaaagg gccactgaat
     6241 gatttttatc ttacacttaa tatttattta tttacttatt tacttttatg tgtgtgtatg
     6301 catgtgtgtg tgtgtgcgtg tgtgtgtgtg tgcatgtgtg tgtatgcatg tgtgtgtatg
     6361 catgtgcgtg tgtgcatgtg tgtgtttgca tgtatgagta tgtgtgcatg tgtgtgtgtg
     6421 tgcatgtttg tgtgtatgca tgtgtgagtg tgtgtgcatg tgtgtgagtg tgtgtgcatg
     6481 tgtgtgtatg catgtgtgtg tgtgcgtgtg catgtgtgta tctgtgtctg agtgcatgca
     6541 agagccagat tagtcagaag cagtattgga cctgggcatg atgagatgct cctcctgctc
     6601 cagccgctcc tcctcacacc caagggtttc tctgtgtagc cctggccgcc gtcctggaac
     6661 tcactctgag gaccaggctg gcctttaagt cagagatcta actgcctctg cctccctagt
     6721 gctgggatga agggcgtgcg ccaccactgc ctggcacact ccagctcctt taatcacagt
     6781 agtcaattgg cagaagcagg aggagccctg tgagttcaag gtagtctggt ctacatagca
     6841 agtttcagaa cagccatggc tacaaagaga ctttgtctca aaaaaaatga aataaataaa
     6901 acattaataa taataataat acaagaggca tcagaccacc tagaactgga gttacagaag
     6961 gttgagtcac tatgtggatg ttgaggacag aacactggtc ccctgcaaga atagacagtg
     7021 cttttaactg ctgagctgtc tcccaagccc cccacacagt gactttttaa aaagaatggt
     7081 aagatttaag tagaggtaaa gagagtacct ctggggagtg aacttaggaa agggtgggag
     7141 cagggagatt ggtttggagg cagttgtagt ctccaggtaa taactaggta atagctaggc
     7201 atatggcggt gatggtgact gccaaatata caaacaaatg ttggactttc agagctggaa
     7261 ggcactcccc ttctggaagg ctggctttaa actgtctatt tttctgcctc catctaccaa
     7321 aagcatgcac caaccacatc caacaagaaa accaccttaa aaacaaaaga aaacaaaaca
     7381 aatcccagtt gtgtatagta gcccacatct atgatcccag cattgggaag tagagccagg
     7441 tggcccactt gtgaagtcca ggccagccta gtctatatag tgaactttaa ctagatggag
     7501 ctgcagagtg agatcctgtc tcaaaagtac caactcacca acctttcaac caacgaacaa
     7561 aaaacagaaa caaaccatct cagaaaataa cccaaaaggt aaaaaataga cacaaataac
     7621 aaagttcaca aagttctgca ttttatacaa aagcaggtta attggctgaa caagaagggc
     7681 attaaatatg tttactggat aaatggagga tttacataga aatgtgtaag ggaaaggaga
     7741 aggtgttgac tttaaaaaac tagcaagaga gagggtctgg agaatgggct cagtggttaa
     7801 gagcactgct cttccagagg acctgggttc aaatcctagg acccacacac acatctgtca
     7861 caagcttcac agaaacataa tacatgcagg gaaaacacca gtgcacataa acgtaaataa
     7921 aaaaccaaaa aactagcaaa aggaagaaag acctgtgaag gtctgagagg tggaaggagg
     7981 ttgtaggaga ggtagactgg aagaaagaac tagaaagagt tcaggggggc agtgatggac
     8041 acactgggct actgggctcc aagggtctgg agggagcaac cttagtctgg aagtgtgtgt
     8101 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgttg gggggggtga ataaaaatgc
     8161 agaaggcagg aaaaggggaa agataaatga gaaccttaga agccaagctg ggaggaagga
     8221 tggtcaaagt tctgggaaag ctggaaggag tggaggctga gctaggcttt ctattttttt
     8281 ttttaagatt ttattattat atttaaatac actgtagctg tcttcagaca caccagaaga
     8341 gggtgtcaag atctcattat ggatggttgt gagccaccat gtgtttactg ggacttgaac
     8401 tcaggacctt tggaagagca gtcagtgctc ttaacccctg agccatctcc ccagccttaa
     8461 gctagacttt ctaagggatg aattaagatg ctagagtggg tcggagggca ccgaaagggc
     8521 acacaaacag gtccccggaa gctcagaatg aaggaaagag aaagtgtgtg gatggagaag
     8581 gaagtgagga aaatgaaggt aaaccagaaa tgacaacctg gggaggggaa tgttgggcgt
     8641 gtcgtgtcag actctctgac ctaggtctta cccttctccc caccctcgga aaactccttc
     8701 gggtagaagg agcctctatg ttggaggaga cactgacagg ctcaggtgct aagtcctgta
     8761 cctacctggt cctgggacca ggcctgtggg aggactgtcg gagagccgtg tcttgtggag
     8821 ggcttgagcg gtgaagcggt aggttgctag actgtctgag agtagaaatt cctagaaggg
     8881 tgcatgcagc acgggtgatt ttaccgtaat gggttagggc ccctgatgat gagacaggcc
     8941 ttggtaggag actaaagaaa aaaagagctg agagtggaga cgaaagtcgg gctggacaag
     9001 tggcacgcgc cgaaacctgc aggggaagga tggcgaggag cctttccagg ggcgtctgag
     9061 ggtactggct ccgcagcgac ccaggcgcac ctgtatggaa aggagaggaa ggggccactt
     9121 ggagtctgca gggcagactc cgaccccagg gacttctgag aagccgtcgc ctatttccca
     9181 tcaacggtta gagccaggca aatgaagggg cgtgaccctg gagctcaggt ttcttcctct
     9241 tcacctggac gaggaggggg taggggccaa gacttccggt tcaggtgagt gtcccttcag
     9301 tgacgtcagg tcactcgact gcccctctgg tcccgcccaa gttgcagcgc tccgggtgcc
     9361 tgcgggggcg cgtccccgac gtcctgcata tatatactca ggtgcgccgc acctgctcgc
     9421 ccgcacctgc cgccgcaccg ccagctccct gtgccgcgca ccgcagcctg gggcccaagg
     9481 gcccgcatac tttctggggg ccacgcccta acggcccctc cacttctttg ggtagtctcg
     9541 gagcacgccg gtaggaaccg cccggccttc ggggaccgct tttgtctgga accagtcctt
     9601 cgggcgaagc cggttccctc tgctgtgaga gtgtcgcttc accggagggg acgatttgtg
     9661 tttacctcgg ttaacaagat ttgtagttac ccggtccaaa aaaaaatctt tttctccttt
     9721 gggtgactta aatctctatc caaattttct tgtgttgctg ctccccggat ttttgttttt
     9781 ttgttttttt gctttactgt agggtcgccc tcccggcgcg tcccgtcttt tctgagacaa
     9841 ggaaatggcc aactcgggcc tgcaactgct gggcttttca atggccatgc ttggctgggt
     9901 gggcctgata gcgagcactg ccatccctca gtggcagatg agctcctatg cgggcgacaa
     9961 catcatcaca gcccaggcca tgtacaaggg gctctggatg gagtgcgtca cgcagagcac
    10021 cggcatgatg agctgcaaaa tgtacgactc ggtgctcgcc ctgccgggta aggccgtggg
    10081 accgacgggg cggacgtgcc cagggtcaag gtggccgggt tgggtgtggg gcagggccca
    10141 ggccgcactg cccggcgccc ggggttgcca gggaggagga agtggacgcc ttgggctcag
    10201 tcggtgggac agagagagag cgtgcctggg atctgatctg ggtgtcccac gtgggtccac
    10261 gcccctctgc ccagattttt tttggggggg tttcctgggc gaagcacccc ccaacccctg
    10321 gcagtcagtg gtctctctct tcccctgctt ctttctctgg acaaaaggag aagagggacg
    10381 gaggttctca gggccgcgga atcgtgccct cgttagacgt gggaccgtac ttgtggcctc
    10441 taatcttatc ggccgattag ctgaagccac aaactcggtg gcctcgatag cggggccaaa
    10501 gctaggacac gcgccggctg gtgtggctgg agcgagggcc ttagccgcca cctgcacttc
    10561 tcttaaggcg cataacgata ccacgatatc aacaagtttg tacaaaaaag caggctggcg
    10621 ccggaaccga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcgaac
    10681 cctttcccac accaccctcc acacttgccc caaacactgc caactatgta ggaggaaggg
    10741 gttgggacta acagaagaac ccgttgtggg gaagctgttg ggagggtcac tttatgttct
    10801 tgcccaaggt cagttgggtg gcctgcttct gatgaggtgg tcccaaggtc tggggtagaa
    10861 ggtgagaggg acaggccacc aaggtcagcc ccccccccct atcccatagg agccaggtcc
    10921 ctctcctgga caggaagact gaaggggaga tgccagagac tcagtgaagc ctggggtacc
    10981 ctattggagt ccttcaagga aacaaacttg gcctcaccag gcctcagcct tggctcctcc
    11041 tgggaactct actgcccttg ggatcccctt gtagttgtgg gttacatagg aagggggacg
    11101 ggattcccct tgactggcta gcctactctt ttcttcagtc ttctccatct cctctcacct
    11161 gtctctcgac cctttcccta ggatagactt ggaaaaagat aaggggagaa aacaaatgca
    11221 aacgaggcca gaaagatttt ggctgggcat tccttccgct agcttttatt gggatcccct
    11281 agtttgtgat aggcctttta gctacatctg ccaatccatc tcattttcac acacacacac
    11341 accactttcc ttctggtcag tgggcacatg tccagcctca agtttatatc accaccccca
    11401 atgcccaaca cttgtatggc cttgggcggg tcatcccccc ccccaccccc agtatctgca
    11461 acctcaagct agcttgggtg cgttggttgt ggataagtag ctagactcca gcaaccagta
    11521 acctctgccc tttctcctcc atgacaacca ggctcccagg tcccgaaaac caaagaagaa
    11581 gaacgcagat cgcagatcgc agatctctag agtcaccgag ttgctttacc ggatgaagag
    11641 ggccgaaaca tactgtccaa ggcccttgct ggcaatccac ccaactgaag ccagacacaa
    11701 acagaaaatt gtggcaccgg tgaaacagac tttgaatttt gaccttctca agttggcggg
    11761 agacgtcgag tccaaccccg gaccggtgca tgtggacccc aagaaaaagc ggaaggtgga
    11821 ccctaaaaag aaacgcaaag tggatcctaa gaaaaaaaga aaggtgcacg tgtccatggt
    11881 gtccaagggc gaggaactgt tcaccggcgt ggtgcccatc ctggtggaac tggatggcga
    11941 cgtgaacggc cacaagttca gcgtgtccgg cgagggcgaa ggcgacgcca catacggaaa
    12001 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccttggc ctaccctcgt
    12061 gaccacactg acctacggcg tgcagtgctt cagcagatac cccgaccata tgaagcagca
    12121 cgacttcttc aagagcgcca tgcccgaggg ctacgtgcag gaaagaacca tcttctttaa
    12181 ggacgacggc aactacaaga ccagggccga agtgaagttc gagggcgaca ccctcgtgaa
    12241 cagaatcgag ctgaagggca tcgacttcaa agaggacggc aacatcctgg gccacaagct
    12301 ggagtacaac tacaacagcc acaacgtgta catcatggcc gacaagcaga aaaacggcat
    12361 caaagtgaac ttcaagatcc ggcacaacat cgaggacggc tccgtgcagc tggccgacca
    12421 ctaccagcag aacaccccta tcggcgacgg ccctgtgctg ctgcctgaca accactacct
    12481 gagcacccag agcgccctga gcaaggaccc caacgagaag agggaccaca tggtgctgct
    12541 ggaattcgtg accgccgctg gcatcaccct gggcatggac gagctgtaca agcctgcagg
    12601 cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg ggccgtccaa
    12661 tttactgacc gtacaccaaa atttgcctgc attaccggtc gatgcaacga gtgatgaggt
    12721 tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg ttttctgagc atacctggaa
    12781 aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc aagttgaata accggaaatg
    12841 gtttcccgca gaacctgaag atgttcgcga ttatcttcta tatcttcagg cgcgcggtct
    12901 ggcagtaaaa actatccagc aacatttggg ccagctaaac atgcttcatc gtcggtctgg
    12961 gctgccacga ccaagtgaca gcaatgctgt ttcactggtt atgcggcgga tccgaaaaga
    13021 aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca ctgatttcga
    13081 ccaggttcgt tcactcatgg aaaatagcga ccgctgccag gatataagga atctggcatt
    13141 tctggggatt gcttataaca ccctgttacg tatagccgaa attgccagga tcagggttaa
    13201 agatatctca cgtactgacg gtgggagaat gttaatccat attggcagaa cgaaaacgct
    13261 ggttagcacc gcaggtgtag agaaggcact tagcctgggg gtaactaaac tggtcgagcg
    13321 atggatttcc gtctctggtg tagctgatga tccgaataac tacctgtttt gccgggtcag
    13381 aaaaaatggt gttgccgcgc catctgccac cagccagcta tcaactcgcg ccctggaagg
    13441 gatttttgaa gcaactcatc gattgattta cggcgctaag gatgactctg gtcagagata
    13501 cctggcctgg tctggacaca gtgcccgtgt cggagccgcg cgagatatgg cccgcgctgg
    13561 agtttcaata ccggagatca tgcaagctgg tggctggacc aatgtaaata ttgtcatgaa
    13621 ctatatccgt aacctggata gtgaaacagg ggcaatggtg cgcctgctgg aagatggcga
    13681 tctcgagcca tctgctggag acatgagagc tgccaacctt tggccaagcc cgctcatgat
    13741 caaacgctct aagaagaaca gcctggcctt gtccctgacg gccgaccaga tggtcagtgc
    13801 cttgttggat gctgagcccc ccatactcta ttccgagtat gatcctacca gacccttcag
    13861 tgaagcttcg atgatgggct tactgaccaa cctggcagac agggagctgg ttcacatgat
    13921 caactgggcg aagagggtgc caggctttgt ggatttgacc ctccatgatc aggtccacct
    13981 tctagaatgt gcctggctag agatcctgat gattggtctc gtctggcgct ccatggagca
    14041 cccagtgaag ctactgtttg ctcctaactt gctcttggac aggaaccagg gaaaatgtgt
    14101 agagggcatg gtggagatct tcgacatgct gctggctaca tcatctcggt tccgcatgat
    14161 gaatctgcag ggagaggagt ttgtgtgcct caaatctatt attttgctta attctggagt
    14221 gtacacattt ctgtccagca ccctgaagtc tctggaagag aaggaccata tccaccgagt
    14281 cctggacaag atcacagaca ctttgatcca cctgatggcc aaggcaggcc tgaccctgca
    14341 gcagcagcac cagcggctgg cccagctcct cctcatcctc tcccacatca ggcacatgag
    14401 taacaaaggc atggagcatc tgtacagcat gaagtgcaag aacgtggtgc ccctctatga
    14461 cctgctgctg gaggcggcgg acgcccaccg cctacatgcg cccactagcc gtggaggggc
    14521 atccgtggag gagacggacc aaagccactt ggccactgcg ggctctactt catcgcattc
    14581 cttgcaaaag tattacatca cgggggaggc agagggtttc cctgccacag cttgatgaag
    14641 atctgagctc ttaattaatg atcataatca gccatatcac atctgtagag gttttacttg
    14701 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
    14761 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    14821 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    14881 tatcttatca tgtctggatc cgtttaaact aactttaaat aattggcatt atttaaagtt
    14941 acatatggcg gccgccctaa ggctcgacct cgaaattcta ccgggtaggg gaggcgcttt
    15001 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
    15061 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
    15121 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
    15181 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
    15241 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
    15301 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
    15361 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ttccggaggc ccggcattct
    15421 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
    15481 cgacctgcat ccatctagat ctcgagcagc tgaagcttac catgaccgag tacaagccca
    15541 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
    15601 tcgccgacta ccccgccacg cgccacaccg tcgatccaga tcgccacatc gagcgggtca
    15661 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
    15721 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
    15781 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
    15841 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
    15901 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
    15961 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
    16021 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
    16081 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
    16141 agcgcccgac cgaaaggagc gcacgacccc atgcatcgat gatatcagat ccccgggatg
    16201 cagaaattga tgatctatta aacaataaag atgtccacta aaatggaagt ttttcctgtc
    16261 atactttgtt aagaagggtg agaacagagt acctacattt tgaatggaag gattggagct
    16321 acgggggtgg gggtggggtg ggattagata aatgcntgct ctttactgaa ggctctttac
    16381 tattgcttta tgataatgtt tcatagttgg atatcataat ttaaacaagc aaaaccaaat
    16441 taagggccag ctcattcctc ccactcatga tctatagatc tatagatctc tcgtgggatc
    16501 attgtttttc tcttgattcc cactttgtgg ttctaagtac tgtggtttcc aaatgtgtca
    16561 gtttcatagc ctgaagaacg agatcagcag cctctgttcc acatacactt cattctcagt
    16621 attgttttgc caagttctaa ttccatcaga agctggtcga gtaactttaa ataattggca
    16681 ttatttaaag ttaggcgcgc cataacttcg tatagcatac attatacgaa gttatgtcga
    16741 gatatttaaa tattagaccc agctttcttg tacaaagtgg ttgatatctc tatagtcgca
    16801 gtaggcggtc gccccctttc tcccttcccc cctacctgga acggtttcat cgaaaccttg
    16861 gcgccccggg cgggttctgg agcgcagggc ggggcgggga gcggaaagga cttggtggcg
    16921 ggatgggggc gggaaccgtc tgcacaaggc ctgccttgga ctagggggcc ccaggctgcc
    16981 ggcggaacca gacaggcgga ccacggtcca ggggttagtg ggcgcggtct gcagcactaa
    17041 ggctggcgcc atcctaaggc tacctgccat ttccagcaat gaccgccgcc ttcctcccct
    17101 gcaggagccc tgcaggccac tcgagcctta atggtggtgt ccctggtgtt gggcttctta
    17161 gccatgtttg tcgccacgat gggcatgaag tgcacacgct gtgggggaga tgacaaagcg
    17221 aagaaggccc gaatagctat gactggaggc attgttttca ttgtggcagg tgagcctcaa
    17281 gggtccaggg tgggaccaag gggctcagtc tttcacccag aagagcatag tcaagatctt
    17341 ggtctggaca cccaatacca gaatcttctt tctctttcta ggtcttgctg ccttggtagc
    17401 atgttcctgg attggtcatc agattgtcac agacttttat aaccccttga cgcccatgaa
    17461 cgttaagtaa gtctccagtc taaagaacca gtaagaacca gattatgctg ggcgtggtag
    17521 cacacgcctt taatcccagt acttgggaga cagaggcagg cggatctctg agttcgaggc
    17581 cagcctggtc tacaaagtga gttccaggac agccaaggct acacagagaa accctgtctt
    17641 gaaagaacaa acaaaaagat tatgtggact tgggagacat caggtccgtt ttgactccaa
    17701 tcttttctcc ctacaggtac gagtttggac ctgccatctt tatcggctgg gcagggtctg
    17761 ctctggtcct tctgggaggt gccctgctct cttgctcctg tccaggcagt gaaagcaaag
    17821 ctgcgtaccg agcaccccgc tcttacccca agtccaattc ctccaaggaa tacgtgtgag
    17881 ctggggagcc cagcctgcgg gcagggtggg aggcaaaggc ctcctggcca ctccacctcc
    17941 aaacatcatg tatagtttgc ttggggggag ggcagttggg gggttactgg gagaggaaga
    18001 catggggtgt gcttttgtac agaaataaaa ttaagttttg gaaattaggt ctttccaggc
    18061 cgctttttct ccagacccag atccttgcac aggacttgga actaaagcaa cttacagcag
    18121 catgtggtac cagtggtgtc tccaggcctg gggtccctag agatggcgca acgcaattaa
    18181 tgataacttc gtatagcata cattatacga agttatggtc tgagctcgcc atcagttcag
    18241 tctcaaggtt cttacccact ctcctccaaa cttttcccca ctgattatta ttgtgtggcc
    18301 tagtggtata taggtgggta tgtgtagggc agtgagttca gggactgaat taaggtcatt
    18361 gggaatgtgg caagtacctt ttcctgttgc tatctcatca tccccaacat ctgctcttgt
    18421 ttaagcatta ccagaggttt tgattgacac acacacacat atatatttta ttagaaatgg
    18481 gcacacacta tgccctcatc acactcatcc tagcttgatg ctgctccttg gctcacgaca
    18541 cagcttctgc agacaaagta acaaaggaca agggacaagg tagagtctgc caacagcagg
    18601 gtggttctga gcccacagcc ttcctctgcc atttgtctgt caaatatcta ggtcatcatc
    18661 tgggtcttct gggtccacat catcaaaagc atctggctca tagaagatgt ggccagtggt
    18721 ccggctgggt accggatgca gcagagcttt ctgtctgagg aaggaagaaa tgatgagggt
    18781 gggtgctaag tcaaatctgg gtagttcctt gtaggtgata ttagcatagg taaccaagcc
    18841 taggtctttg ttccttttcc taaagttttg agagtgatct gtggggatca ctaggtcctg
    18901 gaacaggaac tatcttgtac tgctctaaga gagccaggcc cttagctagg aggcaaacat
    18961 tcttaagtct tgtttcacac cctctcacac acagagcagg aagcatcccc gagctcctga
    19021 gaacaagccc ttgctcatat acactgtggt ttggtccctg ctgcccacct cccccatctg
    19081 gtctggctct cttggcccat ggctactcag ggcccgtggc tttactggag agaaatactg
    19141 aaagggcctc tatcattgtg ggcatttggt ataggtgggt atggaaccac tccctagact
    19201 tgtctccgga ggtggacttc agcccctata aaatcttgag gttaatcaat aacctgtcat
    19261 caccccacgt ttctgtgaca cagatgctaa gatccaagcc caaccttact tttcagagct
    19321 gaactggaaa ggcagagtga ggctgtctct ggcttctctt tctttcttgg ataagtgaag
    19381 gttaaaggtc aaatgagcag tgggatccac ctgtaggtac aagtgttatg atcactcagt
    19441 tctacttttc aagccccaga cccccaacac ctctactttt cttttttttt tggggggggg
    19501 ggtcgagaca gggtttctct atatagccct ggatatcctg gaactcactt tgtagaccag
    19561 gttggcctcg aactcagaaa tccgtttgcc tctacctccc gagtgctggg attaaaggtg
    19621 tgtgccacca ctgcccagct aatatctcta gtttctattt ttccttctgc cagactctag
    19681 gctcacaccc ttgccaattt tgacacctgg gttgtgtgat gatccgggtg tagctcagaa
    19741 cggaggggaa gcccttcatg gagagtcagg ctgaagtcag gaagaacgga gaaccaccaa
    19801 gtctagggaa gaaaaaaaaa tccccttgtt catttgctgt aaggcatgat ccttaattgg
    19861 ttacattaat ggactctttt tttttttttt tttaagacaa tgagtttctc tatttagccc
    19921 tggctgtcct ctcaagtaat ggtatcaaag gtgtgcacca gcatgtttgg ctggcttatg
    19981 ggcccttgag ttgagcaacc ctgagttcaa atcctggttc ttatgcttta ttacttaggt
    20041 gatttaaatt ttaacatatc tgggtttgtt ccccagtctg aataacaagg atgaaacaat
    20101 gacttcaagc accacaaaag ggaacccagc ttagctgtgg tagcacacct ctttaacccc
    20161 agcacttggg aggcagatct gtgtgttcaa agctagcctt aatctaaata aaatcaagtt
    20221 ccaggctgaa aaagctacac agcgagaccc tacctcaaac aaaacaaaat caacacggaa
    20281 gtaattaggt cacctctcca ttattgcctt ccgaggtagg acctcgtttg tcacacagcc
    20341 caggcgacag gtaaccttta attcctgatc ctcctgcctc tacttcccaa gagaacatca
    20401 gtttttattg ctcatcccat tatgctgcct gccctggcca gtttgatatc agtttgacgt
    20461 aagtcatctg gaacctaaat ttcggaaagg cctccctaag aagcagctgt ggctcatttc
    20521 cttgattagt gattaacaga gtgggaaggt ggggaagccc cagccccagg ctccttgccc
    20581 tgtttcatgt ttcagttcct gtcctgacgt ccttcaatga tggactaaaa tgtggatgga
    20641 tgtgtaagcc aaataaaccc tttcctcctt aagttgcttt tggtcgtggt gtttcatagc
    20701 agcaatagaa accctgacta agactctggc ctttcagatc ctaatctcca gtcttctcct
    20761 ttgtctctcc tgacctggta agttgcacgt tgttggggcc ttcgacagag gatggaggct
    20821 gaggtctggt ccactttacc actcagagtc acctctgtgt gagcaagggt gttcagtgct
    20881 cccatggagc ctgggccatg aagctcttca tgtaggaggc ccaggacacg cacctgctct
    20941 actagggagt tatcacctgc aggggcgggg acaagaaata cagatgaaag acatcaggcc
    21001 tgaggagcaa acagtgaacg aagaaaggac ttaccttggc caatcttacc atctccccac
    21061 cccacattgt gcttctgcta gtgcacactt taattatcca atgttggtat gagcagggga
    21121 ccactgactt cgacatgcac aatttatcat gtgcccacaa actttgcata ttaatgacag
    21181 agctgctgta tgctgtgggt gtggcctctt cccccctcta gggatgcatt acctgcacac
    21241 tgtactcaac actttggcac acgtttcttc tttttttttt tttttttttt aaattttttt
    21301 ttttttgatt tttctttttt tttttttttt aaagatttat ttatttatta tatgtaagta
    21361 cactgtagct gtcttcagac actccagaag agggcatcag atctcattac gggtggttgt
    21421 gagccaccat gtggttgctg ggatttgaac tttggacctt cggaagagca gtcgggtgct
    21481 cttacccact gagccatctc accagccccc acacgtttct tcttacattt actttatgga
    21541 cgtttatgtg tatgtcccat ggtgtgcgtg tgctatggct tgcttgtaga gatcagaagc
    21601 taacttttat ttgtttgttt ttcaagacag ggtttctctg tgtagttttg gctatcctgg
    21661 aactcactct gtagaccagg ctggcctcga actcagaaat ccacctgccc ctgcctccca
    21721 agtgctggga ttaaaggcgt gcaccaccac gacaggctag aaactaactt tttgtaaact
    21781 gttcctcctt ctaccatgtg gggtccaagc gttgaataaa ggtcattaga tttggtggta
    21841 agcatcttta cccaccgagc cagcctgttg ggtggcccaa ttgggcacat tttctcttgt
    21901 aattaaaaaa aatgtttaat ttgagataag gtctcactag atggtctgga tcttgaccag
    21961 gttggttctg aacttaagag atcagcctgc ctctctctgc taggattaaa ggtgtgtgcc
    22021 accacagcca ggtttaaaca aacaaacaaa caaacaggtt ttatttggtg atgtgtgtgg
    22081 gtatccagag gcattagagg gtgttggtat ttgatcccct acagctggag aagcaggtgg
    22141 ttgtaggtgt tgggagccaa atttcagtct gttggaagag cagcaagggc tcttaaccac
    22201 tgtcatctct ccagccatca acagttgaat ttttatatag gctagcattt tagaccgaac
    22261 tacacaacaa cttcaactgg cttatcctaa agctatcata ctcttctttt tttttgaaac
    22321 tgggtcttat gttgt
//