LOCUS       119197#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        20504 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 119197
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000024004
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            5933..6000
                     /db_xref="ENSEMBL:ENSMUSE00000686188"
                     /rank="1"
                     /note="ENSMUSE00000686188"
     exon            6117..6239
                     /db_xref="ENSEMBL:ENSMUSE00001333933"
                     /rank="2"
                     /note="ENSMUSE00001333933"
     misc_feature    4275..9807
                     /note="5 arm"
     misc_feature    9835..9859
                     /note="B1 Gateway"
     misc_feature    9872..9919
                     /note="Frt"
     misc_feature    9920..10419
                     /note="mouse En2 intron"
     primer          complement(9988..10009)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9884..9907)
                     /note="FRT3"
     primer          14085..14103
                     /note="seq primer NF"
     misc_feature    14145..14152
                     /note="RE sites"
     misc_feature    15945..15978
                     /note="loxP"
     misc_feature    complement(14153..14184)
                     /note="Rox"
     misc_feature    complement(15905..15936)
                     /note="Rox"
     misc_feature    14185..14206
                     /note="polylinker"
     CDS             14765..15361
                     /note="puromycin N-acetyl transferase"
     misc_feature    14880..14885
                     /note="Mutated RsrII site"
     intron          10420..10794
                     /note="mouse En2 intron"
     misc_feature    10792..10794
                     /note="Splice Acceptor"
     exon            10795..10848
                     /note="mouse En2 exon"
     primer          10695..10716
                     /note="seq primer FCHK2"
     misc_feature    10849..10971
                     /note="1Dleader"
     misc_feature    10972..11023
                     /note="F2A"
     misc_feature    11119..11835
                     /note="EGFP"
     misc_feature    11029..11118
                     /note="3X NLS"
     misc_feature    13901..14139
                     /note="SV40 polyadenylation site"
     misc_feature    12934..13878
                     /note="ERT2"
     promoter        14217..14728
                     /note="PGK promoter"
     misc_feature    15472..15904
                     /note="PGK pA"
     primer          13959..13982
                     /note="LRPCR RAF5"
     misc_feature    11845..11898
                     /note="T2A"
     misc_feature    11895..11895
                     /note="A to G to kill RsRII site"
     misc_feature    11899..12924
                     /note="Cre"
     misc_feature    10810..10849
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    10810..10825
                     /note="FCHK QC prefix region"
     misc_feature    10826..10843
                     /note="FCHK QC critical region"
     misc_feature    10844..10849
                     /note="FCHK QC suffix region"
     misc_feature    16001..16021
                     /note="B2 Gateway"
     misc_feature    9808..16051
                     /note="Synthetic Cassette"
     exon            16443..16560
                     /db_xref="ENSEMBL:ENSMUSE00001287271"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00001287271"
     exon            16651..16763
                     /db_xref="ENSEMBL:ENSMUSE00001243993"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 2 ENSMUSE00001243993"
     misc_feature    16052..17167
                     /note="Critical Region"
     misc_feature    17191..17224
                     /note="Downstream LoxP"
     misc_feature    17168..17247
                     /note="synthetic loxP region"
     exon            20211..20307
                     /db_xref="ENSEMBL:ENSMUSE00001303903"
                     /rank="5"
                     /note="ENSMUSE00001303903"
     misc_feature    17248..20504
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaaagcac cccctggttt cataagctct tctgtttata tcttcctcat
     4321 ttgaaaactg gctgtaaagt tctacccacc aaaactactc aaatctacac catcaagatt
     4381 catcctcagg tgccattcct caaaactcgc cccctggtgc tattctccaa acacagacca
     4441 aagctttatg acttgctctg gtgtgtggaa ctctaagctc taatcatcct ggggggcctt
     4501 aagcttcact cacctgcagc cggccagcac cctccactgc cccgcggagt ggggtagggg
     4561 gtgggagagg gcaaaagacc ccgactaagt agaattaaat acaacaggag gttcggagtg
     4621 tgaggaatac ccagagaaag gacgtgtcgc tagctgcgga tgcgagtgat gaaaagttaa
     4681 aagcggttag ttgtgagttc ttgtggcagg cctccccgtt cctcctcccc gccccgtcca
     4741 gccccgcccc atcttcctaa acactttgcc cctatccccc agagggtcaa gttaaccaaa
     4801 ggaaagaaga aatgctttca aaatgaatct tgttttgctt cctgcagaca acagacgctg
     4861 tcacctggat cctggcttca cattccaaac cagaacatta ctttctttcc agtgttgtgg
     4921 ttacaaacag ttttgttaga atggctttca ttccgtatca ctcactgccc cactgaaagt
     4981 gtacctttcc agaccgggag ctggctgaag gagtttgctg caaaacttga tgaccttact
     5041 ttgattccaa gctccactga ctctgcaaag ttgcccccag atcaccatgg ttctcatgtc
     5101 cccagcctcc caaataaata caattttaaa agtgggccag caactggaat tttattcagc
     5161 aactaaaaaa taacattatg gtccagcact tgggaggcag aggcagaggc agtcggatct
     5221 ctgtgagttc aagacaaacc tggtctacaa agtgagtcca ggacagccag ggctctgtta
     5281 cgcaaagaaa gtctgtctca aaaaaaacaa acaacaaaaa caaacaaaca aacaaacagg
     5341 aaaagaaaaa gaaagaaatt atgaaatttc aggaaaacgt gttaaggcca ggacagagca
     5401 agagagcaag aaaaggcaag atggctccgt ggctcagtga gtaaaggcac ctgccaccaa
     5461 atcagatgac ctttgtttca tccccaagac ccacaaggtg gaaggaaaga atagattgtt
     5521 gtcctctgac cccacagata cgccatgatt tatgtgtgcc ctgttctcat gtacaaaata
     5581 ataataaaaa ttagaaataa taaaactttt aaacgcagct aactataaaa agatccgtga
     5641 ggagatggaa taaaaagctt gtaagggaga gggctgcgga cagtattgga cctcacataa
     5701 taaggtattg ggaggggata ctgattgaga ggcctggctg cataaagtgc ctcaaggttc
     5761 atggggcagg taacctgcag agacagatgt aaaataggac ttggtctatc tgaaggagag
     5821 agaagtccca agtctcacag agctggtaga gaggaggagc aggaggaggg agggagagaa
     5881 gagagggggc aggtcactgc agggtggggc tctgactata aagaatgaag ccagttcact
     5941 gatcccatag gcaatacacg ctacaagcag gcaccagctc tcaggaccag aaaggcattg
     6001 gtgagtcttt ttctgactac agcagaggct agagcaagtc ctttgccatc tgcctctctt
     6061 tcagagagtt ccaggggtgg cttgagatac tgatgtcttt ccttccactt ttataggtgg
     6121 cccccttaaa ccttcaggta tctggagagg agatctaacc ttcactatga aactgtggct
     6181 ttttgcctgc ctggttgcct gttttgttgg ggcctggatg ccggttgtcc atgcccaagg
     6241 tattgtcctt agctatacct accttgcccc ggtttgggca tattgctttc tcttctcact
     6301 tgtccttctc cacttgttga agtctccctg tattctgtat cctttgaact cttcagtgtc
     6361 tcttgggagg ttgggcataa caagaattcc tggtactact ggaggtagat gggtagagat
     6421 gaagtgtccc cagctactta ctcccagatc cctcactttc attctgaagt gcttcctttg
     6481 caagactcaa gaggtttagg ggctgcagga acctgagggt ggattccaat tgaggagaaa
     6541 ttcacttagc cagaaatgca ttttgatgcc cttcttctgc ctagttaggg gatgtgaagt
     6601 taagctcaga accctgaaga ctctccagac tgcattccct gcctttcctc cctgtgtaat
     6661 ctaggcaagg ggctaagatt attctgtgtg ggactcagtg tccccatctg ctaagcagga
     6721 atgaaaattt aaggatccaa tgaactgcaa aatactctca gctgcaacag gattcccagc
     6781 cctgtggtca gggaaccatc tttgtcttct ttcttggcct cctggaaagc aggtactgga
     6841 ggattcagtt acccaaggcc agttctcagg ggcatgaagc atcccccaag agtcctgtca
     6901 ctgggtgctt catctcagaa aaaactgtga agtcccagca ggtgtcaagg caaaatccca
     6961 ggccaggttg aggaccagga tccctgcttt tgccttgcct gctcttctac agacaggtcg
     7021 tgcaccttca agcttccacc gtacccatag aaagccaagc ttggcttttt tttttttccc
     7081 attctgctat ctgggccact cccaacctct gccctttgat cctgggagaa aaatggcccc
     7141 cagtgttgga aaacacctgt cagggatccc tgaagtccca tttcttatct gacaccactg
     7201 ccccttgtcc tcaagagcca aggtggagca atggcaagga ggatgaactc tgtccctgaa
     7261 cctctactag gctctacttg tcctcagatc gaggaaccca ctgtcttgcc aactttcttt
     7321 attcatttgc ttctctcctc agaactttct aggatcatct cctcctcctt gggcacctcc
     7381 tccagctcca gtgcaccttc tttcatgtag ataacatttt tggcttccct accaggttga
     7441 gatactcctg agcattaggt ctaattcatg tctgtgttgt atggctcttt ggtaccaaga
     7501 agaaatcttt aagctcttgt tagactaaca ggggaatcgg caaagaatgg ctaggtcagt
     7561 ttagcagcca ctgagatgag tatagagagg aaaagggatt taagttaatt caacatgggg
     7621 acaagctgac tcatacctgg agctgagatg gtcaggaaag ttgaataacc tgtttatatt
     7681 tttatccaac ttacatttat ttatttattg tgtgcgcgtg tgtgtggatc agtatgcaca
     7741 gtgcccatgt ggagataatt tgcaaagtct ttcctttcac tctgtggatc catggggtca
     7801 aactcaggtt atcaggcttg gtggcaagag ctttctcact atgtgtaact cactgtccag
     7861 gaactcacta tgtagaccag gctagtctca aatgcttaga gatcctcctg agtgctgaaa
     7921 tgaaaggcat gtgccacaca tgtggccttt aaattttttt aattaaaaaa aattttgaca
     7981 gggttttcct gtatagcctt ggctatgctg gaacacactc tgtagaccag gctagccttg
     8041 aactcagaga tctgcctgcc tctgccttct gaatgttggg attaaaagtg tatactgcta
     8101 catctagcta atttttatat ttttaaattg agatagggtc tcatatatct caggctggtc
     8161 tccaactagc ttcctagctg aggatgattt tgaaattctg atctgcctgc ctctacttcc
     8221 caggtgcgag gattctagga atgtactacc gcactcagtt tatgaacttc tggggatcca
     8281 agccagtgcc tcatggaagc taggcaaaca ctttgccaat agtcctagta ccatctaggt
     8341 gctttgagac aggtttcatg tagctcagac tggcttcaaa ctccttatcc tctgtcccag
     8401 ctgggcttaa agatacacat gtgcctcaat gtctggataa ccacttttag gaaattaggg
     8461 ctgtactggt tgtgccccag gtgtgcacct gttctctacc tcagagcaga aatagaaggg
     8521 actttgggct gtttctttgc ctacctacac tctatagttc ctgccagtgc cctgatatca
     8581 agagctgaga acaagccagg aagctttctt tccaaggcag cctggtgacc tctgtatcta
     8641 gaaggtggtg tgtgtgtgtc tgtctgtctg tgtgcacaca catgcatgca cgcacacaca
     8701 aacatgcatg tatgctcaca tctgtggtta tcctgctagt gatgtacacc tggaatcaag
     8761 tgtgtgactg gcttgtcagg ttacaggtac ctttttgtga tttattttgg agaatttcat
     8821 atgtgcaaat atgttttgat caaatccatt ccctattccc ctctccccca atatcacctc
     8881 atcccccacc actttcctca tccagtttca tgatctgtca tctactagaa cattgatggt
     8941 ctttcaggga ttatatccct gaagaaaact gagcctctct tagcagccac cggtttccag
     9001 tagctcttca gccagggtta agacttagtg tgctctgtct ttggagagct ttgggggccc
     9061 ccttctagga atttggcagg aatttgactt tgggctagga caagaaagta ggcacaagat
     9121 gaatacagct gagaaatgtg atctttgtat attgatgggt cttgttaaga ctctgaatat
     9181 aggccgggcg tggtggtgca cgcctttaat cccagcactc gggaggcaga ggcaggtgga
     9241 tttctgagtt cgaggccagc ctggtctaca aagtgagttc caggacagct ggggctacac
     9301 agagaaaccc tgtcttgaaa aaacaaaaac aagcaaacaa acaaaaaaga ctctgaatat
     9361 agtaaccaag gggcctttgt ttgtgctttg tttgttcctt aactatctgt ttatgccttg
     9421 tttgctcctt gactattttg tttatgcttt aggactgacc atattctttg catgtaccta
     9481 gaatgataga aaagcagact ggaaaaaaat gaaccagctt tagcttcaga actggctggg
     9541 gtcatgttat agtgttgtct aattgtcttt ttcttttcag tcctccctcc ctcttggaga
     9601 cctgctgact gactgagctg gcttggtcag ctacccatcc acccttgttt ggattctagc
     9661 tgtcttaatc ttgggtgggt ctgtgcactc attacagtgc agtggccttc tcatgcccag
     9721 caactattct ttccctgagg atgtctactt cctccaagtc ttacagtttt tctgccccca
     9781 ctcttctgag ataatccttg agccttgaag gcgcataacg ataccacgat atcaacaagt
     9841 ttgtacaaaa aagcaggctg gcgccggaac cgaagttcct attccgaagt tcctattctc
     9901 tagaaagtat aggaacttcg aaccctttcc cacaccaccc tccacacttg ccccaaacac
     9961 tgccaactat gtaggaggaa ggggttggga ctaacagaag aacccgttgt ggggaagctg
    10021 ttgggagggt cactttatgt tcttgcccaa ggtcagttgg gtggcctgct tctgatgagg
    10081 tggtcccaag gtctggggta gaaggtgaga gggacaggcc accaaggtca gccccccccc
    10141 cctatcccat aggagccagg tccctctcct ggacaggaag actgaagggg agatgccaga
    10201 gactcagtga agcctggggt accctattgg agtccttcaa ggaaacaaac ttggcctcac
    10261 caggcctcag ccttggctcc tcctgggaac tctactgccc ttgggatccc cttgtagttg
    10321 tgggttacat aggaaggggg acgggattcc ccttgactgg ctagcctact cttttcttca
    10381 gtcttctcca tctcctctca cctgtctctc gaccctttcc ctaggataga cttggaaaaa
    10441 gataagggga gaaaacaaat gcaaacgagg ccagaaagat tttggctggg cattccttcc
    10501 gctagctttt attgggatcc cctagtttgt gataggcctt ttagctacat ctgccaatcc
    10561 atctcatttt cacacacaca cacaccactt tccttctggt cagtgggcac atgtccagcc
    10621 tcaagtttat atcaccaccc ccaatgccca acacttgtat ggccttgggc gggtcatccc
    10681 cccccccacc cccagtatct gcaacctcaa gctagcttgg gtgcgttggt tgtggataag
    10741 tagctagact ccagcaacca gtaacctctg ccctttctcc tccatgacaa ccaggctccc
    10801 aggtcccgaa aaccaaagaa gaagaacgca gatcgcagat cgcagatctc tagagtcacc
    10861 gagttgcttt accggatgaa gagggccgaa acatactgtc caaggccctt gctggcaatc
    10921 cacccaactg aagccagaca caaacagaaa attgtggcac cggtgaaaca gactttgaat
    10981 tttgaccttc tcaagttggc gggagacgtc gagtccaacc ccggaccggt gcatgtggac
    11041 cccaagaaaa agcggaaggt ggaccctaaa aagaaacgca aagtggatcc taagaaaaaa
    11101 agaaaggtgc acgtgtccat ggtgtccaag ggcgaggaac tgttcaccgg cgtggtgccc
    11161 atcctggtgg aactggatgg cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc
    11221 gaaggcgacg ccacatacgg aaagctgacc ctgaagttca tctgcaccac cggcaagctg
    11281 cccgtgcctt ggcctaccct cgtgaccaca ctgacctacg gcgtgcagtg cttcagcaga
    11341 taccccgacc atatgaagca gcacgacttc ttcaagagcg ccatgcccga gggctacgtg
    11401 caggaaagaa ccatcttctt taaggacgac ggcaactaca agaccagggc cgaagtgaag
    11461 ttcgagggcg acaccctcgt gaacagaatc gagctgaagg gcatcgactt caaagaggac
    11521 ggcaacatcc tgggccacaa gctggagtac aactacaaca gccacaacgt gtacatcatg
    11581 gccgacaagc agaaaaacgg catcaaagtg aacttcaaga tccggcacaa catcgaggac
    11641 ggctccgtgc agctggccga ccactaccag cagaacaccc ctatcggcga cggccctgtg
    11701 ctgctgcctg acaaccacta cctgagcacc cagagcgccc tgagcaagga ccccaacgag
    11761 aagagggacc acatggtgct gctggaattc gtgaccgccg ctggcatcac cctgggcatg
    11821 gacgagctgt acaagcctgc aggcgagggc agaggaagtc ttctaacatg cggtgacgtg
    11881 gaggagaatc ccgggccgtc caatttactg accgtacacc aaaatttgcc tgcattaccg
    11941 gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag
    12001 gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg
    12061 tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt
    12121 ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta
    12181 aacatgcttc atcgtcggtc tgggctgcca cgaccaagtg acagcaatgc tgtttcactg
    12241 gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta
    12301 gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag cgaccgctgc
    12361 caggatataa ggaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc
    12421 gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc
    12481 catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg
    12541 ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat
    12601 aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag
    12661 ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct
    12721 aaggatgact ctggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc
    12781 gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg
    12841 accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg
    12901 gtgcgcctgc tggaagatgg cgatctcgag ccatctgctg gagacatgag agctgccaac
    12961 ctttggccaa gcccgctcat gatcaaacgc tctaagaaga acagcctggc cttgtccctg
    13021 acggccgacc agatggtcag tgccttgttg gatgctgagc cccccatact ctattccgag
    13081 tatgatccta ccagaccctt cagtgaagct tcgatgatgg gcttactgac caacctggca
    13141 gacagggagc tggttcacat gatcaactgg gcgaagaggg tgccaggctt tgtggatttg
    13201 accctccatg atcaggtcca ccttctagaa tgtgcctggc tagagatcct gatgattggt
    13261 ctcgtctggc gctccatgga gcacccagtg aagctactgt ttgctcctaa cttgctcttg
    13321 gacaggaacc agggaaaatg tgtagagggc atggtggaga tcttcgacat gctgctggct
    13381 acatcatctc ggttccgcat gatgaatctg cagggagagg agtttgtgtg cctcaaatct
    13441 attattttgc ttaattctgg agtgtacaca tttctgtcca gcaccctgaa gtctctggaa
    13501 gagaaggacc atatccaccg agtcctggac aagatcacag acactttgat ccacctgatg
    13561 gccaaggcag gcctgaccct gcagcagcag caccagcggc tggcccagct cctcctcatc
    13621 ctctcccaca tcaggcacat gagtaacaaa ggcatggagc atctgtacag catgaagtgc
    13681 aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg cggacgccca ccgcctacat
    13741 gcgcccacta gccgtggagg ggcatccgtg gaggagacgg accaaagcca cttggccact
    13801 gcgggctcta cttcatcgca ttccttgcaa aagtattaca tcacggggga ggcagagggt
    13861 ttccctgcca cagcttgatg aagatctgag ctcttaatta atgatcataa tcagccatat
    13921 cacatctgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
    13981 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
    14041 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    14101 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgtttaa actaacttta
    14161 aataattggc attatttaaa gttacatatg gcggccgccc taaggctcga cctcgaaatt
    14221 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
    14281 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
    14341 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
    14401 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
    14461 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
    14521 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
    14581 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
    14641 gtcttccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
    14701 ctcttcctca tctccgggcc tttcgacctg catccatcta gatctcgagc agctgaagct
    14761 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
    14821 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
    14881 agatcgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
    14941 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
    15001 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
    15061 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
    15121 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
    15181 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
    15241 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
    15301 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
    15361 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
    15421 gatgatatca gatccccggg atgcagaaat tgatgatcta ttaaacaata aagatgtcca
    15481 ctaaaatgga agtttttcct gtcatacttt gttaagaagg gtgagaacag agtacctaca
    15541 ttttgaatgg aaggattgga gctacggggg tgggggtggg gtgggattag ataaatgcnt
    15601 gctctttact gaaggctctt tactattgct ttatgataat gtttcatagt tggatatcat
    15661 aatttaaaca agcaaaacca aattaagggc cagctcattc ctcccactca tgatctatag
    15721 atctatagat ctctcgtggg atcattgttt ttctcttgat tcccactttg tggttctaag
    15781 tactgtggtt tccaaatgtg tcagtttcat agcctgaaga acgagatcag cagcctctgt
    15841 tccacataca cttcattctc agtattgttt tgccaagttc taattccatc agaagctggt
    15901 cgagtaactt taaataattg gcattattta aagttaggcg cgccataact tcgtatagca
    15961 tacattatac gaagttatgt cgagatattt aaatattaga cccagctttc ttgtacaaag
    16021 tggttgatat ctctatagtc gcagtaggcg gtttatagct gggaattcca gagtctcttt
    16081 tcctgaaagt tgatcatttg tgggtctctg ggataactgc catctactac aaaaggaagc
    16141 ttctctgatg agggtataac ctatgagcta tacataaaaa tggagtggag agtttattac
    16201 tatgtccttt tattaggtat tagactctag cttagacctt atgactcgtg tgtgtgtgtg
    16261 tgtgtgtgtg tgtgtgtgtg tgtgttgtag ttctcaggta catgtgtgca cctacatgca
    16321 tgtgccacat gtgtatagat gtcagaggat aatttgtgat tcgctcctac tatgtggttc
    16381 ctggggattg aatttaggag gcaagcacct ttacccactg agtcatctca cttgctctgt
    16441 aggtgccttt gaagactgct gcctgggtta ccagcacagg atcaaatgga atgttctccg
    16501 gcatgctagg aattatcacc agcaggaagt gagtggaagc tgcaacctac gtgctgtgag
    16561 gtgagcagga cctccctttg ctgggagggc atcatgcttg ccttagatcc tcatgtccta
    16621 acttggcact catcttccca ccttctgtag attctacttc cgccagaaag tagtgtgtgg
    16681 gaatccagag gacatgaatg tgaagagggc gatgagaatc ttgacagcta ggaaaaggct
    16741 agtccactgg aagagcgcct caggtgggta aggcctcagg ccaacatctc agggagaaaa
    16801 acagcgatta tatatttcct atgcttttta ctttttctcc cttttagcat tgtgcattga
    16861 gtccttcagc attctgggta agtgttctac aactaagata catctctagg ccttccattt
    16921 tgtctttggt gtgtgtgtgc tacatgcaaa tgagtttgca acagttgtgt ttatgacggt
    16981 tgttttattc agggtttctt tttctttctc ttctttttct tttttttttt tttttttttt
    17041 tttttttttg cgagacaggg tttctctgtg tagccctggc tgtactggaa ctcactctgt
    17101 agaccaggct ggcctcaaac tcagaaatcc gcttgcctct gcctcccaag tgctgggatt
    17161 aaggggtgag atggcgcaac gcaattaatg ataacttcgt atagcataca ttatacgaag
    17221 ttatggtctg agctcgccat cagttcagaa ttctttagtc taaggctaag gcctcctggg
    17281 gtccaactgc tcccatctac attagcatgt ctattgttgc aaactccctg ggcccctgac
    17341 tcttaacata tttttgctcc ctcttccaca atgtttcctg agccttaggt gcagggttta
    17401 tagatgtgtc cactgggact aggcacaact ctgccttttg tttagtttag ccttctggag
    17461 tggttgctgt tgcaccaaga agtgttctta gtgagaactg cacttatctg tgggtaacaa
    17521 atgtttagaa tgtcattagg gatggtgctg gcttaggaga gtggtggttg tagggtcttt
    17581 tccaagagcc atgactttgc tgggtttcca gtaccaggca tgatgttgct cttttttttt
    17641 tttttaaaga tttattttta tttattatat gtaagtacac tgtagctgtc ttcagacact
    17701 ccagaagagg gcgtcagatc ttgttacaga tggttgtgag ccaccatgtg gttgctggga
    17761 tttgaactct ggaccttcgg aagagcagtc gggtgctctt acccactgag ccatctcacc
    17821 agcccgatgt tgctcttttt gagaagttct aaagtccaat taaagaactg ttggttacca
    17881 ccaagaagtg tgtcactact gcatacatca tgcctttggg ctatcatgcc tggctggtcc
    17941 ttgtggttca taggtgttgg cctggatagg gttgttggaa cctcgcatgg tgccatgaaa
    18001 cacacctgtt ttgtacaggg catgagggtc aagctcaggt gatgaagctt acagaaaaac
    18061 caattctctc caggcctatc ttgtttttta tttctttgct tttggtttgt tgttgttgtt
    18121 ttgagacaat gtagccctga cactcattat gtagaacagg ttggccttga actcaagaga
    18181 aatctgccca ctctgcttcc caagtgctgg aattaaaggt gtgtgcccgc acacctgcct
    18241 ccactttgtt ttctgagacc acgcctctta ctgggatggg agggtttcca attaggctcg
    18301 ttggtcagtg aactcaagac cttggcttcg tgtctcgtca gtgctgggct tacaaattac
    18361 taccacacct gggttctaca tggacgctag ggacggagct cgggtcctca ggcttttatg
    18421 ataagcactt taccaacttc gctctctctc cccgtacatc cttgttttaa ctactgactt
    18481 aagtttttct ccaggggcta agaagacagc tcacctgtta aagtgcttaa tgtccaagta
    18541 cgaagtttgt atcctaagaa atccatataa accctgggtg gctgtggtgg ttttcctgaa
    18601 tttctagcct cggagcatgg agacatgtgt ttcacataat aaactgtcag ctctgggttt
    18661 aactgagaga tccttcctca gtgaaaaagg tcaaagaaca attgaggata atttctgatt
    18721 ccgcgtgcac atgtgcacat atgaaatgag cccacacacg tgagaaacaa tgtatgcaca
    18781 cacatgcaca ctgtgttaca gctcagagca gagacgtatc ctggcaatta gtcacactac
    18841 acaacaaacc tcaaacaaga aatttattga aaaagacaca ggagggaggg tggcttcctc
    18901 gacagtcttg tgtgagaagg agctgggcag gggacaggct tatatagggt ttctaagagc
    18961 tgaggcagag gtttccagaa tgacctgtga ttggtgggaa tcttgtggtc tgatctcggg
    19021 ggccagctca ggcattgagg agattcctta tttatttatt tatttattta atcttttttt
    19081 ggaaaagttt ttataacgta ttatttattt attttatgta tacaagtata ctgtggctat
    19141 cctcaaacac accagaggag ggcattggat cccattataa atgtttgtga gccacatgca
    19201 tcaaaatata acttgaggaa tcaatgagtt ctactggggt tacttacaga aataggggtg
    19261 aggagttaac ttacaaaggc agaaatgact aaacatacct tcatcaaaac gcaccccgct
    19321 tttttcttgg atcaagaaag ctgaaaaaca ggagtacact ggataccctg caggcagcta
    19381 aacaagctgg agagtgtctc atccaggagg cttggctggt ctggggcgaa cttgcttagc
    19441 taaatttgaa tgttttgaag tagtctttcc tgcttatata cctttgggaa tggagtggca
    19501 gggtgcatct gatcagtttc agggtctttc tgaagctatt caattgttta cttcctgaac
    19561 ctaatgaatt tcctaatgtg tttcacctcc gctcagcata tcctgttgtt tcacctccct
    19621 gcaagttgtg tcttaatagc tttcatctaa gatggcttta agctcagaag aaactgccac
    19681 aaaaggtgtc cttatgtaac cctggctggt tatgagctgg ctatgagcac aaactggctc
    19741 aaactttatc acactgattc agccctgtgc tggggctaca gctttatagc accatgccta
    19801 gctgttagaa ttcttttatt aaaaaataat cacttacctg ggcgtggtgg cgcacacctt
    19861 taatcctagc actcgggagg cagaggcagg cggatttttg agttcgaggc cagcctggtc
    19921 tacaaagtga gttccaggac agccagggct atacagagaa accctgtctc aaaaaagcaa
    19981 aaaaaaattt taaaaatcac ttaaaattaa tttattttaa ttttgtgtat gcatgcttgt
    20041 ttttaagtgt gtgtaccatt cttctggtct ggtgccggtg aaggccagaa gagggcatca
    20101 gaaggtattc aaccctagtc cgctgtaaga gtagcaagta atcttaacca ctgagccatc
    20161 tctacaactc ccattggaac acatgtcttt ttctctctac ttttcaacag actctcagac
    20221 tgaaaggaag aagtcaaacc atatgaagtc caaggtggag aaccccaaca gtacaagcgt
    20281 gaggagtgcc accctaggtc atcccaggta aagactcttg agcacggggc cttcttccac
    20341 caatcacttt gctgacaggg atttactctt aggaacaggg gccttacccg ggactttggt
    20401 cataccctga acttcctagg tggctgttca tccctctacc tggtgtaggg agcatcaata
    20461 aagccaaatg tgaaacatga tctactatca gcaaacagaa agtg
//