LOCUS       46465#pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21167 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 46465
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000026499
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8444..8560
                     /db_xref="ENSEMBL:ENSMUSE00000969850"
                     /rank="3"
                     /note="ENSMUSE00000969850"
     exon            5143..5216
                     /db_xref="ENSEMBL:ENSMUSE00001093883"
                     /rank="2"
                     /note="ENSMUSE00001093883"
     misc_feature    4275..9115
                     /note="5 arm"
     misc_feature    9143..9167
                     /note="B1 Gateway"
     misc_feature    9180..9227
                     /note="Frt"
     misc_feature    9228..9727
                     /note="mouse En2 intron"
     primer          complement(9296..9317)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9192..9215)
                     /note="FRT3"
     primer          13379..13397
                     /note="seq primer NF"
     misc_feature    13439..13446
                     /note="RE sites"
     misc_feature    15239..15272
                     /note="loxP"
     misc_feature    complement(13447..13478)
                     /note="Rox"
     misc_feature    complement(15199..15230)
                     /note="Rox"
     misc_feature    13479..13500
                     /note="polylinker"
     CDS             14059..14655
                     /note="puromycin N-acetyl transferase"
     misc_feature    14174..14179
                     /note="Mutated RsrII site"
     intron          9728..10102
                     /note="mouse En2 intron"
     misc_feature    10100..10102
                     /note="Splice Acceptor"
     exon            10103..10142
                     /note="mouse En2 exon"
     primer          10003..10024
                     /note="seq primer FCHK2"
     misc_feature    10143..10265
                     /note="1Dleader"
     misc_feature    10266..10317
                     /note="F2A"
     misc_feature    10413..11129
                     /note="EGFP"
     misc_feature    10323..10412
                     /note="3X NLS"
     misc_feature    13195..13433
                     /note="SV40 polyadenylation site"
     misc_feature    12228..13172
                     /note="ERT2"
     promoter        13511..14022
                     /note="PGK promoter"
     misc_feature    14766..15198
                     /note="PGK pA"
     primer          13253..13276
                     /note="LRPCR RAF5"
     misc_feature    11139..11192
                     /note="T2A"
     misc_feature    11189..11189
                     /note=" A to G to kill RsRII site"
     misc_feature    11193..12218
                     /note="Cre"
     misc_feature    10118..10143
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    10118..10133
                     /note="FCHK QC prefix region"
     misc_feature    10134..10137
                     /note="FCHK QC critical region"
     misc_feature    10138..10143
                     /note="FCHK QC suffix region"
     misc_feature    15295..15315
                     /note="B2 Gateway"
     misc_feature    9116..15345
                     /note="Synthetic Cassette"
     exon            15721..15808
                     /db_xref="ENSEMBL:ENSMUSE00001008319"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00001008319"
     misc_feature    15346..15976
                     /note="Critical Region"
     misc_feature    16000..16033
                     /note="Downstream LoxP"
     misc_feature    15977..16056
                     /note="synthetic loxP region"
     exon            17790..17935
                     /db_xref="ENSEMBL:ENSMUSE00001008099"
                     /rank="5"
                     /note="ENSMUSE00001008099"
     misc_feature    16057..21167
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaattata ccatgattaa gaggatgtgt tcagaattct tttttttttt
     4321 tcaaaaggaa gagtaaaaac ttttagtgaa taaataacaa acaaataaac atcatatcct
     4381 caatagagaa cattcttggt agttttatgt caacttgata cagccagtgt tatttgagtg
     4441 gaggataccc cagttgagag aacactttta aatgatcagg ctgtatgcag gcaaattttt
     4501 aggaaaattt ctcaattact agttgaggaa gtaggcctca ttccattttg tgtagtgcca
     4561 cttctaggct ggtggcccaa agctctataa gaaagcaagc taagcaaata tttgggaaca
     4621 agtgagtaac aagacatgtc catggtgtct tcatcaaacc tgcctccagc ttccttctgt
     4681 gtttgaggtc ccactttggc ttctctcagt gagatgtgct ttgggacata taaggcatat
     4741 aaaccttctc caagttgttt gtggttatgc tgtttcatca cagcaataat aaccagaact
     4801 aagacataga cttacccagg tttttggaaa aaaaattctg acaggagtga tatgacacat
     4861 tctcacttct aattggaaga tgtattccta acaatatatt tataatgggg taggaaacac
     4921 ttatacagaa agggcttagt cataccatgt tagtgccctg tcatggtaat aactggaagc
     4981 ccttgatatt ctttgaagga cataaaaaga attactagac tcctagtacc tgaaaagagg
     5041 acctacattg atttcgtctc agagaatttg taggtgtgtt tctcagaaca caatgtacta
     5101 aacatcataa tgcatgtttt tgttctcttc tttgttctct agccatggca ttaatgcttg
     5161 tgctgttctt cctggctgct gtactgcccc catcccttct tcaagataac tctcaggtat
     5221 gggtaaagtt tattattttc tcagaacaat ttatgctggt atgaaggcta ttcaagtaag
     5281 caactagtca actacatcac tcttcaattt tttttccttt tgattttttg agtcagggtt
     5341 tctctgtatg acccagaact cactctgtag accaggctgg ccttgaactc agaaatccac
     5401 ctgcccctgc ctcccatgtg ctgggattaa aggtttgtgc caaaactgct cagtcttcaa
     5461 aattttacaa gaccagagtg tgtagtaatc acaaagtact cattgatagt tcagaacagt
     5521 tgacattatt ttaaagttgt ggcaaaagaa cttgtcaccc taattacaag tgaatattaa
     5581 acatggttag ttaaattgat taaattaaaa ttgttatgtt tatgcaatat ctaaagaaac
     5641 aggcatgcat tcattacaca aagttattat caaagaaatg gctagagata aatatatcat
     5701 aataaatttt gtataaagaa gcattaagtt gtatttttgc aatatagttt taatataaat
     5761 attgatatta atttaaaata ccctttcctt tttaatgtgg tcaagagata aaacttacac
     5821 ataatatttg aactatattg ttattggatt tttcatgtct ctggatttat ttcctaatag
     5881 tttctctttt aagaattgtt attgatttat ttaatccatt tcagtgtggt cctcacagaa
     5941 acttcttatt acatttcaaa taacacaact tcaacatata tatatatata tatatatata
     6001 tatatatata tataaaatct cattttcata aatattgtgt gaaagattat ttgtctattt
     6061 tcaattttaa cagtttgcta gaatatagct agggatgata ctaaaaacac ttgcctaaaa
     6121 tgcacaaatc tctgggattg atcctcagca acacagacac acacacacac acacacacac
     6181 acacactcat tttcacacag agctgttaag tacatctaca atgcaaacaa aaaacatgag
     6241 ccaaatatta agtatttata atatgttcct tcatttagat attcccagtg aggattagcc
     6301 atttttttaa atgatggagt atgtgggact agcaaagata ttcccgatct tgttagaaag
     6361 ctactaaaag ctaaaagcct tgcacattgc tgtaaccctc gtactcttct ctggtctggg
     6421 cattggagac aactgaaact cttcaagaat agggagtgcc aattactgga caaacttgtt
     6481 ttggaatata agctttatgg tttagatttt ttcttaccat atccaaatta gaaatctaga
     6541 gactattttt gatctaggtg tttattgatg ttgttggtat tgctagtttc ttaaactgat
     6601 tctgttttat tgcacctttg aactatctat gctccctcca ttccttccta acacctcacc
     6661 tttttcttta gtctactgat atctcttcag agaatttcaa gatacaaata gcagagcaat
     6721 tcagagtcac ctttgatgaa tgaaggtttc tttgagagtt atcatgaaga actgatttct
     6781 ctgacattca tttccattcc tgtcttaaac ttgtgctgtt gtaattatta ggtcatatta
     6841 cctacccaat ataaaccttt taactagttt ttatccttac atttctgtgt taatagtatc
     6901 tctttaaaaa atattaccat tgtctgtcaa ggaaagagaa gcagacttat tgtctcttgg
     6961 agctttcagt aaccaaggaa ggtagaaaaa tgcagaaaat aataatggtt tcataaacta
     7021 tatattatgt ctttagaaag gtaacaacct taagtagggt tttcagttga aatggaatat
     7081 ctaaggagat attcctgagt ggataagcac acttactatt cttgcaaagt atattttggt
     7141 ttctattacc tatcgcaatt tgcctaaatt catctgtaac tccaactcca aaggatctac
     7201 tgtgctcctc tggcctctac atgcactttt ctcatctata caaacatata cacttagtta
     7261 aaaataaata cttttagaaa attatgcctt ttccttaata aataaatatt gaatgttatt
     7321 gttactttac tttataataa aatatgagga ttttccaaca atgacttaaa aacatacatt
     7381 ctttctgtct taaaaatcac tccttgttaa gaaaaaaaaa gaattcacta gcagtgagaa
     7441 agtccttggc tcaaggcatt cattgctgct ttcagaacat aaaacaacag ttagatgtgg
     7501 gcattttgca aatactggca atacagtatg tttagatcta tgtggcaaat gtctgcagaa
     7561 atttgaggga actagggact acatgaaaga gaaccaactt atccaagaag taagataaaa
     7621 tgtatgtgca ggtttgtcca ctcataatta agcaataaca tcctggtcta cactgtagaa
     7681 gaagagagga tttgaagtat acttccatca gcaaggggta ttaatatctc tgccaattct
     7741 tatatcactt aacctacctg tcccacacga acacaaaaat gtgtaactaa atatcagcat
     7801 ctgagttaac aacaggttta tttatgttca ttaaattgta aagccagtaa tactgaagaa
     7861 tttcaaaaac ccattgccta caaattgaca tagatattat attagacaat accctttgga
     7921 gtgatcacaa aatcgtatta catataaaat ttttcttact gtaatagata attgaatcca
     7981 cacatatagt gtgtctagac ttccatagtc tcacaagcct ccaaattctg tatcaaagtc
     8041 tgggtatctc tttcaaagat gtataataaa ttatgtgttt tattccttaa cacttatttg
     8101 ctatagccta attgtgtggg aatgacttaa ggttctctac catgcatgac tatcgttata
     8161 ttttcttaaa taaaatttga tatttgttgc ttagaaaagg tttttacttt ttttttttta
     8221 cttttacttt gacatgccat gaattcatgc tttattctca aaatttctgt ttaaaatgtt
     8281 tctgatgaaa agtaacagat gtttttaagt tttagtttca aggtcaatac ctaatattgt
     8341 ctgagcactt taataatgaa cttcattcat cttagccatc attttcacca ttttatgtaa
     8401 ccttatcata cactaatgtg actttcattt tatttccctt taggagaaca gtcttgagaa
     8461 actttcaacc agtaaaaaat cagtccaaga agagattgta agcaagcaca accaattgag
     8521 acgaaaggtt tctccatctg gcagtgactt actaaatatg gtgagggctc acagcagggt
     8581 agtgcatttg gagagctgtg tttggagcct gctgatagca tctgtgttat ctaagagtct
     8641 gccactattt tagcatctat tttttaaaaa tatgtattgt tttgttcatg tgcatgagta
     8701 tctatgtatg tgtttatata ttatgacgtg catgtaagat cagagctctt tctttccttt
     8761 gtcttcagcc atgatatgat tagagtagca tttactaaat tccctttata acagtggctc
     8821 actggcatta tggagctata tcaattacag ccacagaacc aaaaataaat ctttttactt
     8881 ttctctttag tgataaggca ctgtattatt tcctctccct ctcttaatct agggtcagaa
     8941 cataaccttt catctaaaaa gtcgtctgac ttggatatag atctaaagcc tatatttctt
     9001 ttaaattcat tcaacgtgta aaaagagaaa ctagtattta tgaggtaact ttttacaggt
     9061 gtgaaaataa tcttagtgtg tcccccatag ttagtgctcc ttgtagtaca gcaacaaggc
     9121 gcataacgat accacgatat caacaagttt gtacaaaaaa gcaggctggc gccggaaccg
     9181 aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcgaa ccctttccca
     9241 caccaccctc cacacttgcc ccaaacactg ccaactatgt aggaggaagg ggttgggact
     9301 aacagaagaa cccgttgtgg ggaagctgtt gggagggtca ctttatgttc ttgcccaagg
     9361 tcagttgggt ggcctgcttc tgatgaggtg gtcccaaggt ctggggtaga aggtgagagg
     9421 gacaggccac caaggtcagc cccccccccc tatcccatag gagccaggtc cctctcctgg
     9481 acaggaagac tgaaggggag atgccagaga ctcagtgaag cctggggtac cctattggag
     9541 tccttcaagg aaacaaactt ggcctcacca ggcctcagcc ttggctcctc ctgggaactc
     9601 tactgccctt gggatcccct tgtagttgtg ggttacatag gaagggggac gggattcccc
     9661 ttgactggct agcctactct tttcttcagt cttctccatc tcctctcacc tgtctctcga
     9721 ccctttccct aggatagact tggaaaaaga taaggggaga aaacaaatgc aaacgaggcc
     9781 agaaagattt tggctgggca ttccttccgc tagcttttat tgggatcccc tagtttgtga
     9841 taggcctttt agctacatct gccaatccat ctcattttca cacacacaca caccactttc
     9901 cttctggtca gtgggcacat gtccagcctc aagtttatat caccaccccc aatgcccaac
     9961 acttgtatgg ccttgggcgg gtcatccccc cccccacccc cagtatctgc aacctcaagc
    10021 tagcttgggt gcgttggttg tggataagta gctagactcc agcaaccagt aacctctgcc
    10081 ctttctcctc catgacaacc aggctcccag gtcccgaaaa ccaaagaaga agaacgcaga
    10141 tctctagagt caccgagttg ctttaccgga tgaagagggc cgaaacatac tgtccaaggc
    10201 ccttgctggc aatccaccca actgaagcca gacacaaaca gaaaattgtg gcaccggtga
    10261 aacagacttt gaattttgac cttctcaagt tggcgggaga cgtcgagtcc aaccccggac
    10321 cggtgcatgt ggaccccaag aaaaagcgga aggtggaccc taaaaagaaa cgcaaagtgg
    10381 atcctaagaa aaaaagaaag gtgcacgtgt ccatggtgtc caagggcgag gaactgttca
    10441 ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg
    10501 tgtccggcga gggcgaaggc gacgccacat acggaaagct gaccctgaag ttcatctgca
    10561 ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc
    10621 agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc
    10681 ccgagggcta cgtgcaggaa agaaccatct tctttaagga cgacggcaac tacaagacca
    10741 gggccgaagt gaagttcgag ggcgacaccc tcgtgaacag aatcgagctg aagggcatcg
    10801 acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca
    10861 acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc
    10921 acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccctatcg
    10981 gcgacggccc tgtgctgctg cctgacaacc actacctgag cacccagagc gccctgagca
    11041 aggaccccaa cgagaagagg gaccacatgg tgctgctgga attcgtgacc gccgctggca
    11101 tcaccctggg catggacgag ctgtacaagc ctgcaggcga gggcagagga agtcttctaa
    11161 catgcggtga cgtggaggag aatcccgggc cgtccaattt actgaccgta caccaaaatt
    11221 tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg atggacatgt
    11281 tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc gtttgccggt
    11341 cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg
    11401 ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtaaaaact atccagcaac
    11461 atttgggcca gctaaacatg cttcatcgtc ggtctgggct gccacgacca agtgacagca
    11521 atgctgtttc actggttatg cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg
    11581 caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca ctcatggaaa
    11641 atagcgaccg ctgccaggat ataaggaatc tggcatttct ggggattgct tataacaccc
    11701 tgttacgtat agccgaaatt gccaggatca gggttaaaga tatctcacgt actgacggtg
    11761 ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca ggtgtagaga
    11821 aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgtc tctggtgtag
    11881 ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat
    11941 ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca actcatcgat
    12001 tgatttacgg cgctaaggat gactctggtc agagatacct ggcctggtct ggacacagtg
    12061 cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg gagatcatgc
    12121 aagctggtgg ctggaccaat gtaaatattg tcatgaacta tatccgtaac ctggatagtg
    12181 aaacaggggc aatggtgcgc ctgctggaag atggcgatct cgagccatct gctggagaca
    12241 tgagagctgc caacctttgg ccaagcccgc tcatgatcaa acgctctaag aagaacagcc
    12301 tggccttgtc cctgacggcc gaccagatgg tcagtgcctt gttggatgct gagcccccca
    12361 tactctattc cgagtatgat cctaccagac ccttcagtga agcttcgatg atgggcttac
    12421 tgaccaacct ggcagacagg gagctggttc acatgatcaa ctgggcgaag agggtgccag
    12481 gctttgtgga tttgaccctc catgatcagg tccaccttct agaatgtgcc tggctagaga
    12541 tcctgatgat tggtctcgtc tggcgctcca tggagcaccc agtgaagcta ctgtttgctc
    12601 ctaacttgct cttggacagg aaccagggaa aatgtgtaga gggcatggtg gagatcttcg
    12661 acatgctgct ggctacatca tctcggttcc gcatgatgaa tctgcaggga gaggagtttg
    12721 tgtgcctcaa atctattatt ttgcttaatt ctggagtgta cacatttctg tccagcaccc
    12781 tgaagtctct ggaagagaag gaccatatcc accgagtcct ggacaagatc acagacactt
    12841 tgatccacct gatggccaag gcaggcctga ccctgcagca gcagcaccag cggctggccc
    12901 agctcctcct catcctctcc cacatcaggc acatgagtaa caaaggcatg gagcatctgt
    12961 acagcatgaa gtgcaagaac gtggtgcccc tctatgacct gctgctggag gcggcggacg
    13021 cccaccgcct acatgcgccc actagccgtg gaggggcatc cgtggaggag acggaccaaa
    13081 gccacttggc cactgcgggc tctacttcat cgcattcctt gcaaaagtat tacatcacgg
    13141 gggaggcaga gggtttccct gccacagctt gatgaagatc tgagctctta attaatgatc
    13201 ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct cccacacctc
    13261 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
    13321 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    13381 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgt
    13441 ttaaactaac tttaaataat tggcattatt taaagttaca tatggcggcc gccctaaggc
    13501 tcgacctcga aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
    13561 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
    13621 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
    13681 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
    13741 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
    13801 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
    13861 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
    13921 gcgggcgccc gaaggtcttc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
    13981 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcatcca tctagatctc
    14041 gagcagctga agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
    14101 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
    14161 cacaccgtcg atccagatcg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
    14221 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
    14281 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
    14341 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
    14401 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
    14461 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
    14521 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
    14581 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
    14641 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
    14701 cgaccccatg catcgatgat atcagatccc cgggatgcag aaattgatga tctattaaac
    14761 aataaagatg tccactaaaa tggaagtttt tcctgtcata ctttgttaag aagggtgaga
    14821 acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga
    14881 ttagataaat gcntgctctt tactgaaggc tctttactat tgctttatga taatgtttca
    14941 tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca
    15001 ctcatgatct atagatctat agatctctcg tgggatcatt gtttttctct tgattcccac
    15061 tttgtggttc taagtactgt ggtttccaaa tgtgtcagtt tcatagcctg aagaacgaga
    15121 tcagcagcct ctgttccaca tacacttcat tctcagtatt gttttgccaa gttctaattc
    15181 catcagaagc tggtcgagta actttaaata attggcatta tttaaagtta ggcgcgccat
    15241 aacttcgtat agcatacatt atacgaagtt atgtcgagat atttaaatat tagacccagc
    15301 tttcttgtac aaagtggttg atatctctat agtcgcagta ggcggaatat ggcatacata
    15361 agtgggagga actttggata taggtcatga aactaccaag cagacaatga cacaggaaaa
    15421 aggtactgtg gaaggtatgt gagagagggc atgggaggtt gagcaggcgc tggaagaagg
    15481 gagagaaaag catttaaaaa taaacttatt ataaatgtca caatgaaagt tggtagtata
    15541 tacactaaca taaaatttta gatatatact attatgttta ttcattatga tgaaggtcat
    15601 atggtaccta cagaaatctt gcagtgatat aagatagttc taccagcctg cctgcaaaga
    15661 taatgtgcct ttgaagatgt attattattt atagctatct cctttttttt ctctctctag
    15721 gaatggaact atgatgctca agtgaatgct cagcaacggg cagacaagtg tacattcagt
    15781 cacagtccta tagaactcag gacaactagt atgtgaactc aaaatcactt atgtatgaat
    15841 ggagaataac acaggcgggc ccagtttctt gtattgtatt aacaaaactt ttcaaagtaa
    15901 acacctattt tgatactgtc ttttaaaagt agttaaacat ctctggacca gaatgtctca
    15961 gagaggttcc atattagaga tggcgcaacg caattaatga taacttcgta tagcatacat
    16021 tatacgaagt tatggtctga gctcgccatc agttcatgaa ggatggaact actaattaaa
    16081 ataacatgat ggggcttgaa attagaatct ctttttaatt ttatcctatt agtttttatt
    16141 tatgggtatg tgtgtcttgg gtatatcaat gaacctgtga atgtatgtgc ttaatcagaa
    16201 gagtctctaa agatcaaaag gcattgtatt tttgggaact gaagttacag tggttttaac
    16261 ccatccctta tggatgctac aagttgaatt caattgtgct aaaggaacag gatatgctct
    16321 tatctgttca ggttcttttg caggcttctg taattagaat ttctaaattt tgatcattta
    16381 agccagtatc ttatctacct tgttttgttg ttttcttttt ctttttatat tgtttatttg
    16441 tttgtttgtt tgtttttagt cggggtttct ctctgtattc caggttgttc tggaacagag
    16501 tctgtagacc aggctagcct tgaactcatg gagatctgcc tacctttgct tctagagtgc
    16561 tgagattaaa gacatgtaca tatgtggtag cttatctgat gatgtggctt gttcatgttt
    16621 ataagatata tttttaatta tcaaaaagta ttttatctga gcaactcatt ttcatttttt
    16681 tctcagaaaa taccaaatat tttcattatc tttcattact tcttttattg tttttgattt
    16741 ggtggaatac agatctcact gtatagtaca agatgtcttc aaatacataa tcttgtgtaa
    16801 gacagcaatg tgtatgttcc aaactttgtc ttcatttttg gtgacagcta cttatgtaca
    16861 ttctgggtag ctgataagta tattaagtta aaaataaatt attagacata ctctttacat
    16921 gaggaagtaa tgaaagtatg agaacataaa taaatggact ttgagatata gtacttcttg
    16981 aaacattagg aatggtaatc tctggatgaa tttgaaaatc atttaattat ccattattaa
    17041 ggctgtactt cttagctatc attttaatca cgtagtgttt aaaaagtttt attttaagtg
    17101 ctttaacaat cagttaaagt ataaagtaat ttagaatttg cagagattga gaaaatacta
    17161 atctatttat tgggtgttag aagttgtttt tttctccatt cttggaagaa tatttagcta
    17221 atgaaatttt aaatttataa cttaaggcat gacctataaa tgttaaagta ttgttccttc
    17281 tatttttgat tgttttgtac ttgtaagtaa tgactataaa gaaggcttac acaaaacaat
    17341 cgtgctaaat atgtaacaat tcacataaaa atctctagtt agaacagaag agaaattaaa
    17401 taagttagtt ttttgtcttt ttgaagactc cacatcaaca tttcttgcat atgagacaaa
    17461 catacaatat tacacactcc ctcacacgtt ttcacacagg tatggagagt tcaatgtgtc
    17521 aatgaatcaa cttaaggaga gaagctattt agaaataaga ctgctgtatc tatgtagttc
    17581 atatgctgtt cagcccacaa attgcatgcc tgtggctact gtccagacga atgatcaagt
    17641 ctgaagcatg cctctaatga aataatcaaa agtcattgtg agcaatgtct ttgaagagca
    17701 ggaattctgc tttgtatagc ccctcccccc tctctctctt cttttttctt tcctgctacc
    17761 ttaatgattg gtctatttct ttcccataga tttaaaatgt ggtgagaatt tgttcatgtc
    17821 atcttacctt gtaccatggt cttctgtaat ccaaggatgg tataatgaat ccaaaggtct
    17881 tatatttggt gtgggcccaa agcaaaatgt tagtgtggtt ggacatcata ctcaggtaag
    17941 aaacgatatt ttctctataa atgagaggca aaaaaattgt ttgaattact tgatcagggc
    18001 tgcacatcag ctttgtttgg ctcctgaagg agaatgagaa caatttccca aagttttctt
    18061 tccagaaccc ctgacagctg attgccagac aagtgaggtg actgcattag caagtattat
    18121 acaattgtgc ctccattcag gccactgtca aatagagcct accaaagcta agagagaaag
    18181 taaagtgcta aggggattct gaatggctga ttggaaagaa gatccagata tggtataggc
    18241 agatgtctaa gtcaagggtt ggtttgtttg tttgtccaat aaaacatcat gagaaaaagc
    18301 agagtgggga ggaaagggtt aatttggttt atacttctag gttgctgttc atcaccaaag
    18361 gaagtgagga caggaactta gacactgcag gaacctggaa gcaggagctg atacagatgc
    18421 tatggagggt gttacttgta tgcttcctgt gccttgctca gccctcttac ttatagaatc
    18481 taggaccacc aacccaggga tggcccaaca accatgagct gggaagtcct tcattgatca
    18541 gtaactatga cttacatcta tatcttaggg aggcattttc tcacctgagg cttcttcttc
    18601 tctgatgact ccaactttgt caagttgcta cacaaaacaa cccgtacaac tgtccccttg
    18661 tcaagttgac acacaaccat atgcattact tttaagcctc aaactttttt tgttatttat
    18721 ccccaagatc tcacatttaa atataaataa ctataaaaag tcccacagta tctacaaatt
    18781 caaaagcatt aaaatttcaa tctccttaaa aattcaaaat ctcttctaaa agttcaaagt
    18841 ttttcagtag tccattctgg taaaaaacta agttaaatgt aatgtatttt tcaagaggga
    18901 agaagcacag catgatcaca atctgaaaca agcaactgct aaacaccaac agtttttaaa
    18961 actcaatgcc cagtgtctgg gatccacaca tgaccttctg ggcacttcca aatggcttgg
    19021 atcacctctc catctctgtc cactgtgact caggcagctt gtcttctatg ctaatgctgg
    19081 ctccatttca ctgatgttgc tgttattggt ggtggtggaa tggtatttta atttccaaaa
    19141 tgctgaggtc tgctgctgca actgggctgt aatttcagga atagcttttc ataggctctc
    19201 tttctggtac caagcctcaa attcattgca tgaccccttc agtcctgggc cttcaacttc
    19261 cactgaggtt tcactttcat caatggcctc tcctggcctc taagaatgcc aaactttaga
    19321 tattttccat gaatcttcca taccttcaaa attagtaaca ttgccaagtc tagtaggcag
    19381 ccaaaggtac aactttggcc acataatctg gaatgcagct tctctgtgct ctcagggaaa
    19441 cacttcccag cagatttcac cttagtaatg atagtcactt cttaatcatc actaatctta
    19501 tctccagctg accaacatca actgtcccca aagcgtaaag gtttacttca gtagttctgg
    19561 tgtctaatta gtaacagctg attttttttt ttttttgctc tagctaagaa gaactgcaga
    19621 atcttaaagc aaaatagaaa atgaccctaa tacagtcttt aatcttccct ttgaaatttc
    19681 acatgccagg cctccctctt gggtactgtt caacattctt atcttccaag ctcctaaatc
    19741 ttccctcaga tcttttaata tacaatggct tttctagctc aaagttccaa agtcctttca
    19801 aaatcctccc taaaatatgg tcagggatgt cacagcaata ctgcactatg gtttttacca
    19861 aaatctgcaa tatattcagg gttctctaga gtcacataac ttatggatag aatcaatctc
    19921 tctctctctc tctctctctc tctctctctc tctctctctc tctgtctctt tcacatttat
    19981 atttgagatc ctaaaaggat gtacctcaag ggatactgct acctatttta aatttataat
    20041 gcatttaggg atgtattagg ggtagctata tcatattaca cattattgtg acctaatttt
    20101 tgtttataca tttgtgattc tacaaaggat agtcatatct ttttaggcat tattatcacc
    20161 tagctattgt ttgcatttgg acctgaaata tgatttgtgt caaaatgaca agggatagat
    20221 tatgattggt tgccaacatg atcatatctg gaatgaacta taaccaaaaa agaacacata
    20281 cctgttttct ggatctgaag gctaaaagac aatatgtctt ttttcatgat tttgagtttg
    20341 gaagacacaa gcttttgatg aagatactga ggtgggatca tacacacaca cacacagaca
    20401 cacacacaca cacacacaca cacacacaca cacacacaca cacacacttt ttgatctgta
    20461 tcaacatatg tctttaatcc agatcttgag gcataacttt aatctgggcc atatcttctg
    20521 ctagaggcct atataaggac aatgaaaaaa ggaaagtttc tttcactctt tacctgctta
    20581 tattttcttg tccagcatat tcactggagt ctacttcttt aggattctgg gttatacaca
    20641 agaccagttg aaatatgtat caatgtggta ttgaacaact actagatttt tggacttccc
    20701 attcacagct agtcattgct tggttagttg gattgcagtc tgtaagtcat ttcaataaac
    20761 atacacattc atttcataag ttctctgact ctagagatcc ttgactaatg tagcagcaag
    20821 caactccaat tccttgatct gatcagagat actggaactg aaacctggtt tcaggaagga
    20881 accagaaaat tcaagatcag atctctacac ggaagcactc actgcacaca actatcagga
    20941 aatataaatg attactgagt ggtagccttt gtccagtctt gaccagaaag gtttgcatgt
    21001 cagtcatgaa atctgtccaa ttgggaaggc agatactcct ggccatgtgt tagcattcaa
    21061 ctgaacacct cttctgtgat tctgggagga attcatggac cctttgatgg ctgacgagga
    21121 gtagacataa tatccaggtt actctcttgt aatttattgt catgttc
//