LOCUS       KO-first_condition_ready_121038:121217_MGI:101918        22746 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Akr1b7 targeting project(s): 121038:121217
ACCESSION   unknown
SOURCE      Mus musculus.
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 239511
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            5074..5215
                     /db_xref="ENSEMBL:ENSMUSE00000192313"
                     /note="ENSMUSE00000192313"
     exon            8060..8227
                     /db_xref="ENSEMBL:ENSMUSE00001023904"
                     /note="ENSMUSE00001023904"
     exon            8858..8974
                     /db_xref="ENSEMBL:ENSMUSE00001061621"
                     /note="ENSMUSE00001061621"
     misc_feature    4275..9532
                     /note="5 arm"
     misc_feature    9560..9584
                     /note="B1 Gateway"
     misc_feature    9597..9644
                     /note="Frt"
     misc_feature    9645..10144
                     /note="mouse En2 intron"
     primer          complement(9713..9734)
                     /note="LRPCR LAR3_EN2"
     primer          complement(9609..9632)
                     /note="FRT3"
     primer          13796..13814
                     /note="seq primer NF"
     misc_feature    13856..13863
                     /note="RE sites"
     misc_feature    15656..15689
                     /note="loxP"
     misc_feature    complement(13864..13895)
                     /note="Rox"
     misc_feature    complement(15616..15647)
                     /note="Rox"
     misc_feature    13896..13917
                     /note="polylinker"
     CDS             14476..15072
                     /note="puromycin N-acetyl transferase"
     misc_feature    14591..14596
                     /note="Mutated RsrII site"
     intron          10145..10519
                     /note="mouse En2 intron"
     misc_feature    10517..10519
                     /note="Splice Acceptor"
     exon            10520..10559
                     /note="mouse En2 exon"
     primer          10420..10441
                     /note="seq primer FCHK2"
     misc_feature    10560..10682
                     /note="1Dleader"
     misc_feature    10683..10734
                     /note="F2A"
     misc_feature    10830..11546
                     /note="EGFP"
     misc_feature    10740..10829
                     /note="3X NLS"
     misc_feature    13612..13850
                     /note="SV40 polyadenylation site"
     misc_feature    12645..13589
                     /note="ERT2"
     promoter        13928..14439
                     /note="PGK promoter"
     misc_feature    15183..15615
                     /note="PGK pA"
     primer          13670..13693
                     /note="LRPCR RAF5"
     misc_feature    11556..11609
                     /note="T2A"
     misc_feature    11606..11606
                     /note=" A to G to kill RsRII site"
     misc_feature    11610..12635
                     /note="Cre"
     misc_feature    10535..10560
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    10535..10550
                     /note="FCHK QC prefix region"
     misc_feature    10551..10554
                     /note="FCHK QC critical region"
     misc_feature    10555..10560
                     /note="FCHK QC suffix region"
     misc_feature    15712..15732
                     /note="B2 Gateway"
     misc_feature    9533..15762
                     /note="Synthetic Cassette"
     exon            16181..16258
                     /db_xref="ENSEMBL:ENSMUSE00000293604"
                     /type="targeted"
                     /note="target exon 1 ENSMUSE00000293604"
     exon            17892..18014
                     /db_xref="ENSEMBL:ENSMUSE00001061087"
                     /type="targeted"
                     /note="target exon 2 ENSMUSE00001061087"
     exon            18306..18412
                     /db_xref="ENSEMBL:ENSMUSE00000293591"
                     /type="targeted"
                     /note="target exon 3 ENSMUSE00000293591"
     misc_feature    15763..18527
                     /note="Critical Region"
     misc_feature    18551..18584
                     /note="Downstream LoxP"
     misc_feature    18528..18607
                     /note="synthetic loxP region"
     exon            18882..18963
                     /db_xref="ENSEMBL:ENSMUSE00001036753"
                     /note="ENSMUSE00001036753"
     exon            19409..19492
                     /db_xref="ENSEMBL:ENSMUSE00001031392"
                     /note="ENSMUSE00001031392"
     exon            20026..20108
                     /db_xref="ENSEMBL:ENSMUSE00000192320"
                     /note="ENSMUSE00000192320"
     exon            21809..22105
                     /db_xref="ENSEMBL:ENSMUSE00000366485"
                     /note="ENSMUSE00000366485"
     misc_feature    18608..22746
                     /note="3 arm"
BASE COUNT     5805 a   5571 c   5425 g   5944 t     1 others
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaccttat aaaccatcct tgcgtttcac agttgagtac atgacatttc
     4321 atagtcacaa aagattgttt tcaaatcagg cacagttttt tttttttttt aatgtcgtta
     4381 taaaacacaa ttggtcaaat ccctaaatct gaaagaaaag aaaaggaaga agaagaagaa
     4441 aagtaggcaa cgaggaagaa aggaaaaaca aaggaaggag aaagagaatt tcattaggaa
     4501 agaacctgtc ccagtggcta gcaggtcatt ttcatcctca gggaatcttc ttgccttgca
     4561 atctgacaga aaacgcacag atacaggcat ttctagaaag gtgtccatga cctacattta
     4621 aaaaaaaaaa aaatctttcc acaaggtcac ctgccagttt cctctgtaac agacatccct
     4681 tagttttgtg gccaaggaca gttatttcaa agtagttact gtaaaagatg tctcattttt
     4741 cctcgacatt tttatttgcc tcaaactgca tgagcaccca cgcagttcac tagcgttata
     4801 tccactccca gggcaatgac ccttcccaaa taaacttacc agttcatctt ggatgacctt
     4861 tcaaccatgt cacttaggtt cacaaacctc tgatccgcat acccaataaa gcatgcattc
     4921 tgcccagata gcctttgcac tgtgctgact ttaatctttg tttacgatga tcagtgtggc
     4981 acaagattga catgaagttc ctgttctcat gccccaaccc ttggctgtgg ctgcttgcca
     5041 atgtggtaag agcccgcctc ctttatccag gacataaaaa tgtcaccagc ctccttgtag
     5101 agaaagcagg catttcatct gctcactcag agaactctct gcagcaacca tggccacctt
     5161 cgtggaactc agtaccaaag ccaagatgcc ccttgtgggc ctgggcacct ggaaggtgag
     5221 tgtgcagtct tgggacacct gctgcctttg aggggaggtc tggacatttt cttttctgtg
     5281 cagagcatct atctgccttg ggtttgataa ggccagcatt atcacctgtt gtcagctgct
     5341 ttgggttgca gatgagcctt tcccagaaat gacaatagct tccttggcga atatcaggtt
     5401 taaatttgta caatttttca gggagaaaac tgttttcagg gaagttttct cttagaaagc
     5461 ctttccttgg gactctgagg gtctctgttg gtttgttact ggctgagtga ctgagagaga
     5521 gtatagagtt cttaatgacc ccactcttat gatgcaagac tatgccaaca tctgagtggc
     5581 ctgggagctt gtgggaaagg cagaggacaa ctggactggc ccaggcagtt tacaaaatct
     5641 ccatgtgatt cccccacacc ttaagaccca tgcagcatga gagccacaga attggagtca
     5701 gctcaaagtc tgccctggct gtgttaatct ctactggcaa gttgctctgt ggcccatccc
     5761 agtacccagt gaaacagatt tctcacgaaa tcttgggaca agtctgacat tggtccatct
     5821 tcttctaact ctgccttctt tgctgtttct attactgaca acatgagagt agccagcttg
     5881 ttaaccagga gctgttttta gttttgattt tgatttcttg agacagggtt tctctctctc
     5941 taacctaaaa tgaccttgat caaatttgga gtaatcctcc tgcctcagct tcttgaatac
     6001 tgggattata ggtgtgagcc ttcatccttg gctcaggagt agtttgggaa cccatctgtc
     6061 ttagttaggg ttttactgct gtgaatagac accacgacca aggcaagtct tataaaaaac
     6121 aacatttaat tggggctggc ttacaggttc agaggtttag tccattatca tcaaggtggg
     6181 agcatggcag tatccaggca ggcatggcac aggcagagct gagagttcta tgttttcatc
     6241 caaaggctgc tagtggaaga ctgacttcct ggcaactggg gtgagagtct taagtccaca
     6301 cccacagtga cacacctact ccaaccaggc cacacctcca gacggtgcca ctccctgctc
     6361 caagaatata caaaccatca caccatctgt cctgtgtcca agactcattt ccagtcttcc
     6421 tctgtttctc ttttctttac acagagggta ggttctctct ttgtttctac attagattgg
     6481 ggtcttacta gaatggtgag acttgcaacc agagtagaaa tttcacattc cctcaaggtg
     6541 acaaataatt acaaatctcc aggatactca ccctggacaa atttagaaga aatgcacttt
     6601 cagagttcca aaagcatttg taaatatttg taagaagttg ttttcgaggt aaggactctg
     6661 tgctcaccag tggacaagca ggagggtgac actttggccc tgggccccag tgcttttgtg
     6721 tttctggaaa ttccttctcc tcatgatggc agagctgttg atctctgctc tctgtgcttc
     6781 tcaaggtttg atgcttaaaa acagaacctt tagatttagt aataggggaa gagttattta
     6841 tgggctgttg tttgagagtc ttccacagcc ctggttacct tataataatt cattatgtgg
     6901 ctgagactgg ccctgaactc ctggttttcc tactcacctc ccaaatgcta ggattaaaac
     6961 atgggccacc acatccagtg agatgttgtt ttgtattgac tgagataata ttgtgcccca
     7021 ggctgtcctc aaagccacag cctggccctc ctacctttgt gcctctcatg gtggtgttac
     7081 agccatgcag caccatgctc tgcagagagg tacgtgatga gtacttactc tcctaagcca
     7141 atacctggct tggttaggtc aggacagaag gtgacccact tcaaatgctc cttcccttgg
     7201 ctccttggct tgttttctct ttctttctct ctctttctct ctctctctct ctctgtctgt
     7261 ctctgtctct gtctctgtct ctgtctttgt ctctgtctct gtctctgtct ctctctctct
     7321 ctctctctgt agtccatatg gggaaaatcc atttttataa agtttctgtt aggtttaaaa
     7381 ctgaggacaa agaaatgaag tgcctattta acatcttcct gtttgctctt tttctgaaac
     7441 agtgattaca tattggattg caagtgccag gtgatgtgct gaaatcttca caatcttgtg
     7501 gcaagctggc aacacaaagg cctcatgggg cagggcccat aacttgctat gttttgctgt
     7561 caaggtttag ggaaattgag gaatctgctc agcctcatac tgcaccatag cagagttcaa
     7621 atcacattgc tataatatat tttttaaaga tccttctaac tgtctggagt ctgagaactc
     7681 ctcctcagaa tctagttttc aggctagcct tgcaagtttc aaggggagtc atttgtcact
     7741 cagaaactgg aggcacttca agatcaacct ggactggagg actagaatag gacacgagtc
     7801 agaatcatga caatgacatg tgtattaaga tgccataatg aaatccatca ctttgtatga
     7861 taacctaaaa atttagtgaa ataaaaaaag taattcactt tttcttttcc tgggagaaac
     7921 ccctcagtgt atgttttctt ctcccaagtc cacttgggaa caggactgca gcaaggctgt
     7981 ggttttcaga atctggtcta tgtggagccc ttaagaagag gccttggagc atctaatggt
     8041 cttttctatt gcctttcagt cttccccagg ccaagtcaag gaagccgtga aggcggccat
     8101 tgatgctggg tatcgccaca ttgactgtgc ctatgtgtat cacaatgaga atgaggtggg
     8161 agaagctatc caagagaaga tcaaagagaa tgctgtgaag cgggaggatc tcttcatcgt
     8221 cagcaaggta caatgatgca cttggtggac aagacttcaa ggcaggtgga agtatctgtg
     8281 taggggcagg tttgagagct tattcgcctt tctttgtggg cctgcatgtc tcctggccct
     8341 ttgttccctg ctttcattat gtctcttggt agaattccac acacaaaaac atccactgtt
     8401 ggagtggcca cacctatata tgtgtatata ggtatatgtg tatatgctca ttgccattca
     8461 tcacagggcc tgacaggcct ggggataagg gagtactaaa aagggccagc cctcctattg
     8521 catcgtaata cttgaacttg aagagccaca aacaactcct gccatctgag aaatgagagc
     8581 cacagaaaag catgtgacac aagaataatt ctagtcaacg gccgactaag ttaaatgact
     8641 aagaaacaaa cccaatatcg agagcatagg ggaacactta ggaagtgatg ttgacttgct
     8701 aagagctgat gataagaaat acgttgcatt tcttaacgta tttcttataa gtgaatagcc
     8761 ggtcctgggg tgtacaaatc tccaagcagg aggagtaact gtagtatgtg tggccttcct
     8821 ctccctagtt ctaatgctcc cctgttacac cctgcagctg tgggccacat tctttgagaa
     8881 aagcctggtg aagaaagcct tccagaacac cctctcggat ctgaagctgg actatctgga
     8941 cctgtatctg gtccactggc cacagggatt tcaggtttga gctgctcttt gccttctatt
     9001 tttggggaga tgctacaaag tggctatggt gccaagactg tgacactggg gagggatgaa
     9061 gcagggggtt gggtttgagt tttattctat tttttgtata tgagcgtttt gtttacatgt
     9121 atatgtgtgc accatgccta tgcttggtgc ctgtggagat cagaagagag catgggatcc
     9181 ctgggaattg gagttatgga tggttgtgaa acaccacctg ggtgctagga acccaacctg
     9241 ggtcctctgc aagagcagca agtgctctca tcttctgacc cacctctcta gtccccagaa
     9301 gactgtgcag cgcggaggct gaggcaacag gtggtacttg gtgagactca tgcttggttt
     9361 tctgcaattg tgtttgaaag aaacagtatt tcttctacca gctcagataa gctgcaactg
     9421 gccagccatc ttcctcttgg atgcatgccc atgagtgccc ttggtaggat gtcttggggc
     9481 actcttgcat aagttcctat taatcaaagt gtgcccagag agtgacctcc ataaggcgca
     9541 taacgatacc acgatatcaa caagtttgta caaaaaagca ggctggcgcc ggaaccgaag
     9601 ttcctattcc gaagttccta ttctctagaa agtataggaa cttcgaaccc tttcccacac
     9661 caccctccac acttgcccca aacactgcca actatgtagg aggaaggggt tgggactaac
     9721 agaagaaccc gttgtgggga agctgttggg agggtcactt tatgttcttg cccaaggtca
     9781 gttgggtggc ctgcttctga tgaggtggtc ccaaggtctg gggtagaagg tgagagggac
     9841 aggccaccaa ggtcagcccc ccccccctat cccataggag ccaggtccct ctcctggaca
     9901 ggaagactga aggggagatg ccagagactc agtgaagcct ggggtaccct attggagtcc
     9961 ttcaaggaaa caaacttggc ctcaccaggc ctcagccttg gctcctcctg ggaactctac
    10021 tgcccttggg atccccttgt agttgtgggt tacataggaa gggggacggg attccccttg
    10081 actggctagc ctactctttt cttcagtctt ctccatctcc tctcacctgt ctctcgaccc
    10141 tttccctagg atagacttgg aaaaagataa ggggagaaaa caaatgcaaa cgaggccaga
    10201 aagattttgg ctgggcattc cttccgctag cttttattgg gatcccctag tttgtgatag
    10261 gccttttagc tacatctgcc aatccatctc attttcacac acacacacac cactttcctt
    10321 ctggtcagtg ggcacatgtc cagcctcaag tttatatcac cacccccaat gcccaacact
    10381 tgtatggcct tgggcgggtc atcccccccc ccacccccag tatctgcaac ctcaagctag
    10441 cttgggtgcg ttggttgtgg ataagtagct agactccagc aaccagtaac ctctgccctt
    10501 tctcctccat gacaaccagg ctcccaggtc ccgaaaacca aagaagaaga acgcagatct
    10561 ctagagtcac cgagttgctt taccggatga agagggccga aacatactgt ccaaggccct
    10621 tgctggcaat ccacccaact gaagccagac acaaacagaa aattgtggca ccggtgaaac
    10681 agactttgaa ttttgacctt ctcaagttgg cgggagacgt cgagtccaac cccggaccgg
    10741 tgcatgtgga ccccaagaaa aagcggaagg tggaccctaa aaagaaacgc aaagtggatc
    10801 ctaagaaaaa aagaaaggtg cacgtgtcca tggtgtccaa gggcgaggaa ctgttcaccg
    10861 gcgtggtgcc catcctggtg gaactggatg gcgacgtgaa cggccacaag ttcagcgtgt
    10921 ccggcgaggg cgaaggcgac gccacatacg gaaagctgac cctgaagttc atctgcacca
    10981 ccggcaagct gcccgtgcct tggcctaccc tcgtgaccac actgacctac ggcgtgcagt
    11041 gcttcagcag ataccccgac catatgaagc agcacgactt cttcaagagc gccatgcccg
    11101 agggctacgt gcaggaaaga accatcttct ttaaggacga cggcaactac aagaccaggg
    11161 ccgaagtgaa gttcgagggc gacaccctcg tgaacagaat cgagctgaag ggcatcgact
    11221 tcaaagagga cggcaacatc ctgggccaca agctggagta caactacaac agccacaacg
    11281 tgtacatcat ggccgacaag cagaaaaacg gcatcaaagt gaacttcaag atccggcaca
    11341 acatcgagga cggctccgtg cagctggccg accactacca gcagaacacc cctatcggcg
    11401 acggccctgt gctgctgcct gacaaccact acctgagcac ccagagcgcc ctgagcaagg
    11461 accccaacga gaagagggac cacatggtgc tgctggaatt cgtgaccgcc gctggcatca
    11521 ccctgggcat ggacgagctg tacaagcctg caggcgaggg cagaggaagt cttctaacat
    11581 gcggtgacgt ggaggagaat cccgggccgt ccaatttact gaccgtacac caaaatttgc
    11641 ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg gacatgttca
    11701 gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt
    11761 gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct gaagatgttc
    11821 gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaactatc cagcaacatt
    11881 tgggccagct aaacatgctt catcgtcggt ctgggctgcc acgaccaagt gacagcaatg
    11941 ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa
    12001 aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc atggaaaata
    12061 gcgaccgctg ccaggatata aggaatctgg catttctggg gattgcttat aacaccctgt
    12121 tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact gacggtggga
    12181 gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt gtagagaagg
    12241 cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct ggtgtagctg
    12301 atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg
    12361 ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact catcgattga
    12421 tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga cacagtgccc
    12481 gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag atcatgcaag
    12541 ctggtggctg gaccaatgta aatattgtca tgaactatat ccgtaacctg gatagtgaaa
    12601 caggggcaat ggtgcgcctg ctggaagatg gcgatctcga gccatctgct ggagacatga
    12661 gagctgccaa cctttggcca agcccgctca tgatcaaacg ctctaagaag aacagcctgg
    12721 ccttgtccct gacggccgac cagatggtca gtgccttgtt ggatgctgag ccccccatac
    12781 tctattccga gtatgatcct accagaccct tcagtgaagc ttcgatgatg ggcttactga
    12841 ccaacctggc agacagggag ctggttcaca tgatcaactg ggcgaagagg gtgccaggct
    12901 ttgtggattt gaccctccat gatcaggtcc accttctaga atgtgcctgg ctagagatcc
    12961 tgatgattgg tctcgtctgg cgctccatgg agcacccagt gaagctactg tttgctccta
    13021 acttgctctt ggacaggaac cagggaaaat gtgtagaggg catggtggag atcttcgaca
    13081 tgctgctggc tacatcatct cggttccgca tgatgaatct gcagggagag gagtttgtgt
    13141 gcctcaaatc tattattttg cttaattctg gagtgtacac atttctgtcc agcaccctga
    13201 agtctctgga agagaaggac catatccacc gagtcctgga caagatcaca gacactttga
    13261 tccacctgat ggccaaggca ggcctgaccc tgcagcagca gcaccagcgg ctggcccagc
    13321 tcctcctcat cctctcccac atcaggcaca tgagtaacaa aggcatggag catctgtaca
    13381 gcatgaagtg caagaacgtg gtgcccctct atgacctgct gctggaggcg gcggacgccc
    13441 accgcctaca tgcgcccact agccgtggag gggcatccgt ggaggagacg gaccaaagcc
    13501 acttggccac tgcgggctct acttcatcgc attccttgca aaagtattac atcacggggg
    13561 aggcagaggg tttccctgcc acagcttgat gaagatctga gctcttaatt aatgatcata
    13621 atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc
    13681 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
    13741 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    13801 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgttta
    13861 aactaacttt aaataattgg cattatttaa agttacatat ggcggccgcc ctaaggctcg
    13921 acctcgaaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
    13981 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
    14041 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
    14101 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
    14161 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
    14221 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
    14281 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
    14341 ggcgcccgaa ggtcttccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
    14401 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcatccatct agatctcgag
    14461 cagctgaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
    14521 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
    14581 accgtcgatc cagatcgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
    14641 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
    14701 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
    14761 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
    14821 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
    14881 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
    14941 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
    15001 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
    15061 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
    15121 ccccatgcat cgatgatatc agatccccgg gatgcagaaa ttgatgatct attaaacaat
    15181 aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag ggtgagaaca
    15241 gagtacctac attttgaatg gaaggattgg agctacgggg gtgggggtgg ggtgggatta
    15301 gataaatgcn tgctctttac tgaaggctct ttactattgc tttatgataa tgtttcatag
    15361 ttggatatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt cctcccactc
    15421 atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga ttcccacttt
    15481 gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag aacgagatca
    15541 gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt ctaattccat
    15601 cagaagctgg tcgagtaact ttaaataatt ggcattattt aaagttaggc gcgccataac
    15661 ttcgtatagc atacattata cgaagttatg tcgagatatt taaatattag acccagcttt
    15721 cttgtacaaa gtggttgata tctctatagt cgcagtaggc ggtttttgct gtaggccctt
    15781 atgaacagcc ttggtgagag tggcatggtc tccaaactca ggccttctcc aggttgcaag
    15841 gttaggagaa gctggttact ctcttcctca gataaccata ctcttctgat ataaaacctc
    15901 tgcctagtga ccttgaagtt tacattggtt aacttccacg atgatagtga tggcttctat
    15961 aatatactag aggacttgga gccagctgag tattgccaga aatagaggac tgacttccca
    16021 tggtagactg acacaggaat tttttttgaa tattatattt ttatgtgagt tctctggaat
    16081 ttgacatggg tacttcttat ttcgccgtcc tgagacgtct cttcacagga ccaggtaact
    16141 aaatgccgat tctggtgata tttttgtttc tcatttacag gctgggaatg cgttattacc
    16201 caaagacaat aaaggcaaag ttctcctgag taaatccaca ttcttggatg cctgggaggt
    16261 aggttccaac ttccttcaag tgtgctgctc aaagttaaaa tacaaatctt ctctcagatg
    16321 ctgttgagac tccaccctgg tatggaaagg ctcaagtgct ctgtatataa tttcagaatg
    16381 cttattctct agttccccct ctgtcctgga aagtggtgtc tgtggagcta cttgtgaaag
    16441 tcagagggca gctacccaaa gtaggttctt tccttctatc acataggctc caggaagaaa
    16501 ctgtgaccat ctagcttggc agcaaggacc tttacccact gaaacatttc cctggcccca
    16561 tgaaacactt tcaggaacaa caacaacaac aaaaaaacca accaaccaaa caaacaaaac
    16621 cttactgcaa gtcagaggct ctgatagata caagaaggat caagtatgtc agtaaatgaa
    16681 aaataaaata aaaaggaagg acatgactgc tcttaggtat gctggaggag aaagtttact
    16741 cagagtattc caagttgtag ccaaagatga gatttcacgc gtcagctaaa ccctccttgc
    16801 tcgtccctgc ctctaagccc ttgctgtccc tcccccatcc gtgcatgatt ttactacctt
    16861 tcttcatgtc aatttttttt caggctcaat ttaaactctc ctcccaatga ctctcctggg
    16921 ctaagatgat tccttcctct cctccctcat ccatgtacta attgatgtca aataagctga
    16981 ggcagcctag caagcacatt gtatacattt acacacacac acacacacac acacacacac
    17041 acacgtatat gtatatatat atatgtatat atatatatat acacatgcac aatatgatgt
    17101 atatgtagta tatgtatatg catatgtata cacacatata aacagaaact tagaatggat
    17161 gaataattat aaattcacat tcaatgtcac tatcaggttg gataattatc tttgggaaag
    17221 tttaaaacaa gctcaatgta tttaagtaat aaaaaaataa actccagatc cttgggtgga
    17281 tgtgatatgg gttggctggg tgcccctgcc tgggtccatc tgtctgcaag gtgatcctta
    17341 ctgtatgctt tcaatcacgg ctatgtgaga ggctgaggca caaagatcac atttcagatc
    17401 ctgcctggac ttcagagtaa cttcaaggcc aacccttaca agatcctatt caagattgga
    17461 aaatgaaggg ggttaggaac tagagataca gattcacggc agattgcctg tctaacacat
    17521 aggagaccac agactcaacc cttaataagc acaaaataaa aatcaaaaac caaagagaga
    17581 ccacagggac gattcctttc cttacactgt ggactgtttc ctggataacc tacatcactc
    17641 agtttgggtc acttttgcct atgagcagac agcatataaa accgttactt ctccttcctc
    17701 attacatctg agctggggaa gagtaggatg gctggaagca gccatgaggg caccacagtc
    17761 tagactaaaa ttctccttta gtgcttcact taatttgcca cgagggagcc attcctccta
    17821 ccaaggacta agcacgccag catccctgta tcctcttaga gccatctaaa tgatattctt
    17881 cactgtggca ggccatggag gaactggtgg accaggggct ggtgaaagct ctgggcatct
    17941 ccaacttcaa ccacttccag attgaaaggc tcctgaacaa gcctggacta aaacataagc
    18001 cagtgaccaa ccaggtaagt tctgtttggg gtgggtcctg ctctgtgcct gcttcagcac
    18061 tgggaggcag agtctcctct cagaggtcta tgttcaggat gcttagggag gtgggaggca
    18121 ggtctctggg tctggtttgt agcatacata cctgtccatg ggttgtgttt ggcctttgtg
    18181 ttggtccttt catgtgtatg gagttcctgc tgagccattc aggaagacct ccttccctag
    18241 agatggtgat gtttgttttt tttttttttt tttttgtttt tttttttttt tgttttctct
    18301 tgcagattga gagccaccct tatctcaccc aggaaaaact gatccaatac tgtcaatcca
    18361 agggcatcgc tgttacagcc tacagtcccc tgggctcccc agacaggcct tagtaaggct
    18421 tcagcatact ggttctttct tttttttttt ttatccttct taaaattagc attttgtaat
    18481 ttgttctatt agtatgtaag ttccaaggca caagataaaa tataccagag atggcgcaac
    18541 gcaattaatg ataacttcgt atagcataca ttatacgaag ttatggtctg agctcgccat
    18601 cagttcataa cccaagtggg attgtcaggg taaagtgttt ttaaaatgat agcatgtctg
    18661 atcccctgga atattccatt tctgatgtcc tgtgctgggg cagtattttc aaattgccaa
    18721 agtactagat cctatgcacg aatccagggg gaaagatctt cctgcctgcc ttgcagagga
    18781 aaaatgctgc agtgctcagc cttgatgctg tcctggcagg gatgttcacg ggcacacagt
    18841 agccatggtg atttttactc cagctctctt tcttcgtcta gtgccaagcc agaagacccc
    18901 gtagtaatgg agattcccaa gatcaaagag attgctgcaa aacacaagaa aacagtagct
    18961 caggtaaaat aaagtatttc ccccttttat ttagcaatcc agtcttgtag tcatgtgtac
    19021 aactgtgttg cttgctttta attccctgga gaggaagaaa tgggaagtgt aagcctgttg
    19081 ggagcttcag aaaataccgc tagcattagt gaagcaagga tctgcctctg tctttcagat
    19141 tcctgccaca ccctcaggcg ttgggttcaa gcgccaacta tatagggtct aagcctatga
    19201 ctacaggcta ctctcctcac tcctcgttag gaattcctag ttgtttcttg gatcaagggg
    19261 cataagaagg gaactggaaa agtatcccta atggtgaagg gctttgttaa tatcaactgt
    19321 tgttaagcca gttacttagt tgtcatctta caagaaggag aaggtcttgt agctaactgg
    19381 gcacatgcct tcccttcgta atacacaggt tctgattcgg ttccatgtcc aaaggaatgt
    19441 ggtggtgatc cccaagtctg tgacaccctc acgcatacag gagaacctgc aggtaagacc
    19501 tgggctgtac atctgacatt cctcagtgga acaggtgata ggagggaagg aggactcttc
    19561 ttccctgccc ttcagcttct catcccactc tttcactgac agtgtgtctt cccttcttcc
    19621 aagcactctg tcaacttgta ggctagaaag ctgggatgac tgagctcaca ggtggtaccg
    19681 gccaaacctg tcaggactga tcacaactgt agcttatatc actgagatga ccactgccta
    19741 ccattgagca cttagatcat aggtggaatg ctgtgctagc caactgaaga ctgccttgta
    19801 gacatggact ggacttgaaa acttgtcctc tgagacaccc ttcaggcata catacttagc
    19861 acacctacaa cccaatctct tccctcttag aacactaaac tctataaata actcactctt
    19921 aatttagaga ataatgtatt tgtcatggac atattagggg aaacacctca gatccctcca
    19981 gcactttcct tgctaagggt ggttttggaa ctcttgtctt tctaggtctt cgacttccag
    20041 ttgagtgagg aggacatggc tgccattctc agcttcaaca ggaactggag ggcctgtgac
    20101 ctgttggagt aagtggtacc aggttaagta ggaggttgat taggcctctc cctaagggta
    20161 aagggttagt aggaagggat ggaaggcatg tgggattatg ggatggtttt tgtcttcaaa
    20221 tactattttc tggcagttgt gaaaagttaa aaagaaaagt ctgagtcctg gggactcact
    20281 aagggcagag acattgtcat ctgatgctca gtttctgaat cttacacaca gagtgaggat
    20341 tttaaagaaa ggagctgtca ctgctgtgtg ggagtcaagt tctccttccc tcactcattc
    20401 acccagtgac ttcctgtaat gtgctatggc cattcatggt ctacatgttt gggacatcac
    20461 actataagct tcctgttccc aagctcatat tctacagagg acaactgaag taactctgat
    20521 ctcagttaca tacaccaatc agagagtgac aaataccata tagaaaagtg aagcaggaca
    20581 aaaggtatag ctgccctttg taatagacac tttaatacat gactatccag gggaagcttt
    20641 gttcaaaaag tggcagttga agacagtgga agatgagtaa catggctatt tagggaaata
    20701 gcattcttgc aatcatagca accattgcag atgctctgct ctcgtagcta gagcatttga
    20761 atgggattga gaatactgtg gaatgaataa gtgttattgt aagaacgcag aaacttgagg
    20821 agatttaggt taaggctatg gacaggcctt tgatttttag gcctgaaaga ggaataagcc
    20881 atgaacagag ctggtatgat ccgatgtgtc ttaaaaggct cacaatgact tgtgggttga
    20941 gagactacga cagtaaccca tggaaaagat gacagtcgct ggacctggac catggtgttg
    21001 gggaggagat gaggaagacc accatctaga taaatgggaa catgcaatca atttagtttc
    21061 ctcctcaaaa tttacagttg aagctggttt tgtatttttg tttgcgtggt tggcttttgt
    21121 ttgagcaact cacatgcaga attaacattt tggtaaaatc aagggtttga catgcgttta
    21181 ttatgcctgt tgggtatcaa ctaggagtat atgtgaagct agtgaatttg gtgacctcag
    21241 aagccaactg tcactgggtt ctaatccaaa atctactact tattcactag cactggggcc
    21301 cttggggagt tttggtgatg ttctaaaatc acttttctaa tctacaaagt aaggatgaca
    21361 atattaatca cagccaacct tcactgaatt ctctcgttta gcatgcagca gacactttaa
    21421 gccctttgct tatattagta tcatatttac tactcatacc caatctattg atttcaatag
    21481 tatttcctgc ttcacagcaa tgccagagtt tggacaatag ttctggggcc cttagttgtg
    21541 tgtctgcttg tttatgatcc cttaagatat caatgaactg gtgaccgagt gactgatagc
    21601 tcgcaggcct tccttcctta atactcctct gatattttgg gagaaggtcc tgttgtgtgc
    21661 ccgcgccaca ctccatgtcc catgttcaca gtcttgttgg ggaagagatc agaagagtac
    21721 agaaatagct gaatatatca cgtaagagtg tctcagatca catttttgat tctgtggact
    21781 gttttcttct ttgtatttgt tgttgcagtg caaggactga agaggactat cctttccacg
    21841 aggaatactg aggtccactt gcttgatgag atccgtgcat gatagattcc ttcgtctctc
    21901 taaaatcaac ctggatgtag gctaccatct ggcaatgcag gatttaaatt tttgtgttca
    21961 tcaaggagca agaaaatgtt ccagagaaac gaagggcaac ggttctcact agcgagggct
    22021 gagcacatgt ttgtcatcag gagcatgtag tatggtaaca ctgaagatac aatgatagaa
    22081 caataaaaaa gcaataatag taattcctgt agtatgctgt ccatttctgt gataaccact
    22141 atgaccaaaa gcaacttgta gaggaaaggg cttatttgtc ttacatgttt catccgtagt
    22201 ccatcacaga aggaagccat agcagaaaca gaggcaggag ctgggtcgct gaagcatagc
    22261 tcatggagga atgctgctca gtggattgtt ccatggctta tccagctttc ttttcttttc
    22321 tttttctttt tttctttttt tttttttttt ttggtctttt ttgagacagg gtttttctgt
    22381 atagccctgg ctgtcctgga actcactttg tagaccaggc tggtctcgaa ctcagaaatc
    22441 cgcctgcctc tgcctcccaa gtgctgggat taaaggcgtg caccaccacg cctggctttc
    22501 agcttgcttt cttatacaac ccagcaccac ttgcccaggg gtggcatcac ccacacagga
    22561 ctgggctctc ctagaccaat aaatcactaa ttaagaaaat cccctacaga cttgtccaca
    22621 ggccatctga tggagtcaat tcctcaactg aggttctctc ttcctaggtg acttcagttt
    22681 gtgtcaaggt gacaaaaact aatgagcatg gtcaccaaag catctgacac aatttgaaat
    22741 actttc
//