LOCUS       KO-first_condition_ready_35610_MGI:1344416        21327 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Ash2l targeting project(s): 35610
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 48847
COMMENT     backbone: L3L4_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(125..378)
                     /note="BGH pA"
     CDS             complement(503..1159)
                     /note="DTA"
     promoter        complement(1173..1685)
                     /note="PGK promoter"
     promoter        1686..1836
                     /note="spectinomycin promoter"
     CDS             1837..2847
                     /note="SpecR"
     misc_feature    2995..3587
                     /note="pUC ori"
     misc_feature    3858..3885
                     /note="rrnB T2"
     misc_feature    4017..4060
                     /note="rrnB T1"
     misc_feature    4156..4176
                     /note="B3 Gateway"
     misc_feature    4182..4189
                     /note="AsiSI"
     misc_feature    1..4214
                     /note="Synthetic Backbone"
     exon            5483..5628
                     /db_xref="ENSEMBL:ENSMUSE00001208351"
                     /rank="3"
                     /note="ENSMUSE00001208351"
     exon            5296..5359
                     /db_xref="ENSEMBL:ENSMUSE00001218605"
                     /rank="2"
                     /note="ENSMUSE00001218605"
     exon            4592..4789
                     /db_xref="ENSEMBL:ENSMUSE00000736732"
                     /rank="1"
                     /note="ENSMUSE00000736732"
     misc_feature    4215..9050
                     /note="5 arm"
     misc_feature    9078..9102
                     /note="B1 Gateway"
     misc_feature    9115..9162
                     /note="Frt"
     intron          9163..10037
                     /note="mouse En2 intron"
     misc_feature    10035..10037
                     /note="Splice Acceptor"
     exon            10038..10210
                     /note="mouse En2 exon"
     misc_feature    10222..10815
                     /note="ECMV IRES"
     gene            10816..13876
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13915..14158
                     /note="SV40 polyadenylation site"
     misc_feature    14162..14195
                     /note="loxP"
     promoter        14222..14741
                     /note="human beta actin promoter"
     gene            14767..15561
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15761..16002
                     /note="SV40 polyadenylation site"
     misc_feature    16019..16066
                     /note="Frt"
     misc_feature    16073..16106
                     /note="loxP"
     misc_feature    10052..10129
                     /note="FCHK std_gpr"
     misc_feature    16122..16142
                     /note="B2 Gateway"
     misc_feature    9051..16172
                     /note="Synthetic Cassette"
     exon            16346..16434
                     /db_xref="ENSEMBL:ENSMUSE00000210858"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000210858"
     misc_feature    16173..16612
                     /note="Critical Region"
     misc_feature    16636..16669
                     /note="Downstream LoxP"
     misc_feature    16613..16692
                     /note="synthetic loxP region"
     exon            21124..21199
                     /db_xref="ENSEMBL:ENSMUSE00000210868"
                     /rank="8"
                     /note="ENSMUSE00000210868"
     exon            19737..19832
                     /db_xref="ENSEMBL:ENSMUSE00000210867"
                     /rank="7"
                     /note="ENSMUSE00000210867"
     exon            18626..18721
                     /db_xref="ENSEMBL:ENSMUSE00000210869"
                     /rank="6"
                     /note="ENSMUSE00000210869"
     exon            17418..17512
                     /db_xref="ENSEMBL:ENSMUSE00000210870"
                     /rank="5"
                     /note="ENSMUSE00000210870"
     misc_feature    16693..21327
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatatctg
       61 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      121 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      181 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      241 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      301 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      361 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      421 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      481 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      541 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      601 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      661 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      721 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      781 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      841 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      901 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
      961 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1021 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1081 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1141 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1201 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1261 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1321 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1381 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1441 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1501 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1561 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1621 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1681 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1741 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1801 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1921 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1981 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2041 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2101 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2161 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2221 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2281 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2341 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2401 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2461 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2521 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2581 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2641 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2701 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2761 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2821 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2881 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4021 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4201 aatttcacac aggatgtttg gaagtttcct acgttttagg gttttagtat gatcgcctgc
     4261 tccgcacagt gtaaagggga gtctctcaca tcctcatatg tgccgtgatt tccactctta
     4321 gccacgacta tgagcaggcg ttttgaataa ttcaagtccc aagaggaaag caaaggacag
     4381 acctcctaag tggctccctt caaacctggg caatccgagt tgcgccctgc ctttttcttt
     4441 cttcctttct tttttttttt ttttctggcc ttaaagcggc ggactcaaag tgcgtccttc
     4501 ccaagtaagt ggcgggcccc ccaacaaaag gtctttccca ctttaagagc gccgtgcgtg
     4561 gcggcaactc gccgcataga tatattctcg cgagagcttg ggaaggagca gcgatggcgg
     4621 cggctggagc gggtcctggt ccgggagtca gcgccggccc tggtcccgga gccgctgcaa
     4681 gtgccacaac ggcggaggac cgagagacag agccggtggc cgcgggggct ggcgaggggc
     4741 cgtcagccgc cccgggagcg gagcccagct ccggagaggc cgaaagtggg taaggcaatc
     4801 cactcgcagc cccggggacg cgcgagggcc cgcccctcgt ggaagcccgc ggctcgcgcc
     4861 gcccggtgtc gccctccgga tccaggtccc gcagccaacg gctcggcccg cccgcccacc
     4921 cggtaccctc cgcaaccccg ctggctgctt gcttgtggac tccaagccgc tcctcggctc
     4981 ctcggctccc atcagcacca gtgtgagcca agagcgtttc cccctctagc gccccggaag
     5041 acctcgctga tccctcttgc aatgcttggt cctgaaagca ctgaggacgg cagcctcccg
     5101 tcgttagccc agtgcactag gaatccctgg gccgcgaaca catttgtctt gcttgacaac
     5161 ctccctccta atactcagga ttgttctctc gtgggcatcc cgttcaggac atgaaaaaga
     5221 tataccctcc gtatttggac cattgtgttt ttctgtttag gaaaggccat tttgatgttt
     5281 gtcttttcat tatagggatg caaacttggt tgatgtaagt ggtctggaga cagaatcttc
     5341 taatggaaaa gatactttgg taagtatttc ctctgaggcc tttggaaggc gaactagatt
     5401 tggttttaaa atgtttaatc tatttttttt tttttaaaaa gtagtcaggc catttgattc
     5461 tcttattgcc ctgtggctcc aggaaggtac cggagacact tcagaagtga tggataccca
     5521 ggcgggctct gtggatgagg agaatgggcg gcagttggga gaagtggagt tgcagtgtgg
     5581 gatatgtaca aaatggttca ccgctgacac ctttggaata gacacgtcgt gagtacattt
     5641 catggccttc tagacagcat gatcctggag agatggctta gcattttaga tcactagtta
     5701 ccctttcagg ggtttgggct tgattcccag tattcacatg gcagctcaag gaaccccagt
     5761 tctggggtgg aaggggaaat tatgtaatca tatttctttt tttttaatta atttatttat
     5821 tttatgtata tgagtacact gtagctatac aaatggttgt gagccatcat gtggtttctg
     5881 gaaattgaac tcaggacctc tgcccgctct agccctgctt gctccaactc tgctcacaac
     5941 agcccaaaga tttattatat gtgtacactg tagctgtctt cagacacacc agaagagggt
     6001 gtcagatttt tgttaggagt ggttgtgagc caccatgtgg ttgggatttg aactcaggac
     6061 ctttggaaga gcagtcagtg ctcttaactg ctgagccatc tcaccagccc tgtaatcata
     6121 tttcaatctc aagacaattg agaagtattt ttaaatatac cgaaaaaaag tcagtaggta
     6181 gtattttgtg ctcactctaa ccctatccag tgaccagatg ctgactgagg agttgcttgc
     6241 attcctttgg catgttctga agtattgagg tgagagtgct attaggtatg agtgtgggca
     6301 tcgtccttgc ttttgacctt acaggctgat tgggaaggag tctgtgcaat tagaagagat
     6361 tatgtaatta agtttgcgat aatgtattct gtgtgttctg ggcttggtat ttacagtggg
     6421 caagacctgt tgtcagtaac atttgaaggt gtggtgttga gtcgtggtgt acttgcctgc
     6481 tgcgtgcaaa gccctgggtg gtcactgcag agaagtgctt ttaattcctc aaaaatattg
     6541 aggtgatcgg tgcttttcat gtctaaagca tactttgttt cctttctcat tgcttataat
     6601 gtaccccaat tggtatctta tgttttctct caggaaaaaa acaaaacaaa caaacaaaac
     6661 ttacatctaa acatgtacct gttcttgaga attaaaatca tatcataatt cagagcaaaa
     6721 ggtctctttt ggtggaggcg taggtggtca gtaggtatta acgagcattt tgatggataa
     6781 ctattaagtc aatggctctc taactatata tagccctgga tggcctgagg tgcccttttt
     6841 tgaccaagtt ggccttaaaa ttgcaggaat cctctggcct ttgagccagt ctttgttcat
     6901 tttgaggcca ggctgggctg catagttgaa catcaggcca gccagagctg tgccttaagt
     6961 ctttgtctgg aaggggacaa attatattga gtggagctgg ttgtgcacac ctgtaattca
     7021 ggtacttagg aggccggagc aggaggatca caagttccag gttatatgaa ccatagctaa
     7081 tgcaagacca ggggcctatc tcagaaaaag ccaatgtctt agtcagggtt tctattcctg
     7141 cacaaacatc atgaccaaga agcaagttgg ggaggaaagg gtttattcgg cttacacttc
     7201 catgctgctg ttcatcacca aaggaagtca ggactggaac tcaagcaggt cagggagcag
     7261 gagctgatgc agagaccatg gagggatgtt ctttactggc ttgcctcacc tggcttgctc
     7321 agcctgcttt cttatagaac ccagactacc agccctgaga tggtcccacc cacaaggggc
     7381 ctttccccct tgatcactaa ttgagaaaat gccttacaat tgcatctcat ggaggcattt
     7441 cctcaactga agctcctttc tctctgataa ctccagctgt gtcaagttga cacaacacta
     7501 gccagtacaa ttgacccctt gtcaacttga cacacaaaaa catcactagt aagcctcaac
     7561 ccttacaatc ttattcatcc ccattctaaa taactttaaa agtcccacag tctttacata
     7621 ttcttaaaat ttcaatctct ttaaaatatc catttctttt aaaatccaaa gtctttttac
     7681 aattaaaagt ctctcaactg tgggctccac taaaacagtt tcttccttca agagggaaaa
     7741 tatcagggca cagtcacaat caaaagcaaa agtcaatctc caaccgtcca atgtctggga
     7801 tccaactcac gatcttctgg gctcctccaa gggcttgggt cacttctcca gccaggccct
     7861 ttgtagcaca cgcatcatcc tctaggttcc agatgcctgt actccactgc tgctgctgct
     7921 cttggtggtc atctcatggt actggcatct ccaaaacact gcatgacccc ttcagtcctg
     7981 ggccatcaat tgcaactgag gctgcacttt caccaatggc cttccatggc ctctcacagt
     8041 gccaagcctc agcttctctg ctcaactcct tcatgccttc aaaaccagta ccacctgggt
     8101 gacccttaca cattaccaag tccagccaca gcacaatgta caactttggc tatatctgga
     8161 acacagccac tgtgctctca gaaaacactt cccagaagat gtcacctcaa tgatgctggt
     8221 ctcttcttaa tcaccgctaa tttcttagct ccagctaacc agcatcaata gtcccagtaa
     8281 tgcaaagttt ttgctttagt agttctggta tcttgttaat cacagctgat tcttcagccc
     8341 cagctaacca gaactacaga atcttcacaa tcaaaacagc aatggccctg aaaagagtct
     8401 ttaattttcc ctctgaaatt tcacaaacca gacctccatc ttctgcagtg ttctcaacgt
     8461 tgtcttccaa gctcctacac aacatccgac agagctctta acaacggatg gatcttcaag
     8521 cccaaagttc caaagtcctt ccacagtcct ccccaaaaca tggtcaggtt gtcacaggaa
     8581 taccccactc tgctggtacc aatttgtctt agtcagggtt tctattcctg cacaaacatc
     8641 atgaccaaga agcaagttgg ggaggaaagg gtttattcgg cttacacttc catgctgctg
     8701 ttcatcacca aaggaagtca ggactggaac tcaagcaggt cagggagcag gagctgatgc
     8761 agagaccatg gagggatgtt ctttactggc ttgcctcacc tggcttgctc agcctgcttt
     8821 cttatagaac ccagactacc agccctgaga tggtcccacc cacaaggggc ctttccccct
     8881 tgatcactaa ttgagaaaat gccttacaat tgcatctcat ggaggcattt cctcaactga
     8941 agctcctttc tctctgataa ctccagctgt gtcaagttga cacaacacta gccagtacag
     9001 ccaatgagtg tgctagtgag gtagtcagca gatagacact tcctgagtaa aaggcgcata
     9061 acgataccac gatatcaaca agtttgtaca aaaaagcagg ctggcgccgg aaccgaagtt
     9121 cctattccga agttcctatt ctctagaaag tataggaact tcgaaccctt tcccacacca
     9181 ccctccacac ttgccccaaa cactgccaac tatgtaggag gaaggggttg ggactaacag
     9241 aagaacccgt tgtggggaag ctgttgggag ggtcacttta tgttcttgcc caaggtcagt
     9301 tgggtggcct gcttctgatg aggtggtccc aaggtctggg gtagaaggtg agagggacag
     9361 gccaccaagg tcagcccccc ccccctatcc cataggagcc aggtccctct cctggacagg
     9421 aagactgaag gggagatgcc agagactcag tgaagcctgg ggtaccctat tggagtcctt
     9481 caaggaaaca aacttggcct caccaggcct cagccttggc tcctcctggg aactctactg
     9541 cccttgggat ccccttgtag ttgtgggtta cataggaagg gggacgggat tccccttgac
     9601 tggctagcct actcttttct tcagtcttct ccatctcctc tcacctgtct ctcgaccctt
     9661 tccctaggat agacttggaa aaagataagg ggagaaaaca aatgcaaacg aggccagaaa
     9721 gattttggct gggcattcct tccgctagct tttattggga tcccctagtt tgtgataggc
     9781 cttttagcta catctgccaa tccatctcat tttcacacac acacacacca ctttccttct
     9841 ggtcagtggg cacatgtcca gcctcaagtt tatatcacca cccccaatgc ccaacacttg
     9901 tatggccttg ggcgggtcat cccccccccc acccccagta tctgcaacct caagctagct
     9961 tgggtgcgtt ggttgtggat aagtagctag actccagcaa ccagtaacct ctgccctttc
    10021 tcctccatga caaccaggtc ccaggtcccg aaaaccaaag aagaagaacc ctaacaaaga
    10081 ggacaagcgg cctcgcacag ccttcactgc tgagcagctc cagaggctca aggctgagtt
    10141 tcagaccaac aggtacctga cagagcagcg gcgccagagt ctggcacagg agctcggtac
    10201 ccggaagatc tggactctag agaattccgc ccctctccct cccccccccc taacgttact
    10261 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
    10321 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
    10381 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
    10441 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
    10501 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
    10561 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
    10621 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
    10681 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
    10741 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
    10801 aagcttgcca caaccatgga agatcccgtc gttttacaac gtcgtgactg ggaaaaccct
    10861 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
    10921 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
    10981 tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct
    11041 gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc
    11101 tacaccaacg tgacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg
    11161 acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg
    11221 cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc
    11281 ggttacggcc aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc
    11341 ggagaaaacc gcctcgcggt gatggtgctg cgctggagtg acggcagtta tctggaagat
    11401 caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact
    11461 acacaaatca gcgatttcca tgttgccact cgctttaatg atgatttcag ccgcgctgta
    11521 ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct
    11581 ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc
    11641 gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa
    11701 ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac
    11761 ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat
    11821 ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat
    11881 catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg
    11941 aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac
    12001 acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc
    12061 atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc
    12121 gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg
    12181 aatgaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat
    12241 ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt
    12301 tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc
    12361 atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg cgaatacgcc
    12421 cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat
    12481 ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat
    12541 gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc
    12601 cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa
    12661 gcaaaacacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc
    12721 agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat
    12781 ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg
    12841 attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc
    12901 gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag
    12961 tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc catcccgcat
    13021 ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac
    13081 cgccagtcag gctttctttc acagatgtgg attggcgata aaaaacaact gctgacgccg
    13141 ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc
    13201 cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa
    13261 gcagcgttgt tgcagtgcac ggcagataca cttgctgatg cggtgctgat tacgaccgct
    13321 cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat
    13381 ggtagtggtc aaatggcgat taccgttgat gttgaagtgg cgagcgatac accgcatccg
    13441 gcgcggattg gcctgaactg ccagctggcg caggtagcag agcgggtaaa ctggctcgga
    13501 ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat
    13561 ctgccattgt cagacatgta taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc
    13621 gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc
    13681 agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa
    13741 gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg
    13801 agcccgtcag tatcggcgga attccagctg agcgccggtc gctaccatta ccagttggtc
    13861 tggtgtcaaa aataataata accgggcagg ggggatctaa gctctagata agtaatgatc
    13921 ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct cccacacctc
    13981 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
    14041 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    14101 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgg
    14161 aataacttcg tatagcatac attatacgaa gttatgttta aacggcgcgc cccggaattc
    14221 gccttctgca ggagcgtaca gaacccaggg ccctggcacc cgtgcagacc ctggcccacc
    14281 ccacctgggc gctcagtgcc caagagatgt ccacacctag gatgtcccgc ggtgggtggg
    14341 gggcccgaga gacgggcagg ccgggggcag gcctggccat gcggggccga accgggcact
    14401 gcccagcgtg ggcgcggggg ccacggcgcg cgcccccagc ccccgggccc agcaccccaa
    14461 ggcggccaac gccaaaactc tccctcctcc tcttcctcaa tctcgctctc gctctttttt
    14521 tttttcgcaa aaggagggga gagggggtaa aaaaatgctg cactgtgcgg cgaagccggt
    14581 gagtgagcgg cgcggggcca atcagcgtgc gccgttccga aagttgcctt ttatggctcg
    14641 agcggccgcg gcggcgccct ataaaaccca gcggcgcgac gcgccaccac cgccgagacc
    14701 gcgtccgccc cgcgagcaca gagcctcgcc tttgccgatc ctctagagtc gagatccgcc
    14761 gccaccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
    14821 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
    14881 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
    14941 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
    15001 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
    15061 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
    15121 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
    15181 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
    15241 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
    15301 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
    15361 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
    15421 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
    15481 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
    15541 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
    15601 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
    15661 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
    15721 tcttcgccca ccccccggat ctaagctcta gataagtaat gatcataatc agccatatca
    15781 catctgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
    15841 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
    15901 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    15961 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgggggtac cgcgtcgaga
    16021 agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcgtcg agataacttc
    16081 gtatagcata cattatacga agttatgtcg agatatctag acccagcttt cttgtacaaa
    16141 gtggttgata tctctatagt cgcagtaggc ggcacaggat tggcctccac gtcgttcgga
    16201 gctgtgcacc ctgggacatg tacaccctgg gtcatgtgcc caaatatgca ccatgggtac
    16261 acatgctact actaataaat gttttaaagg aagacaaaaa tgtgttgagt ggctcactaa
    16321 tgtgtgtaat ctggcccatc cacaggtcat gtctgccttt tatgaccaac tacagcttcc
    16381 attgcaatgt gtgccatcac agtgggaata cctactttct ccggaagcaa gcaagtaaga
    16441 acagttctag agtattttaa aaagatggtt gtcctttgga aaacaggaga gatctggatt
    16501 agcacactta ggataccttt gcctaagagc tggttggaat tactttgata tttagctctg
    16561 aataattgca cttacccatt tcaaatcaaa ttattacttc actcttagct gagagatggc
    16621 gcaacgcaat taatgataac ttcgtatagc atacattata cgaagttatg gtctgagctc
    16681 gccatcagtt caaaacaact taaaggaagg tttgcagctc caggttacac tccatcacgg
    16741 ctccacagtg tgcacatgac agttcagctc acactctgtc atggctccac agtgtgctca
    16801 tcacagttca gttcacactc tgtcatggct ccacagtgtg cacatcacag ttcagctcac
    16861 actctgtctt ggctccacag tgtgctcatc acagttcagt tcacactcca acacggctcc
    16921 acagtgtgca catgacagtt cagctcacac tctgtcatgg ctccacagtg tgcacatgac
    16981 agttcagctc acactctatc atggcttcac agtgtgcaca tgacagttca ggttacacac
    17041 catcacggct ccacagtgtg ctcatcacag cagcaggcat ccgaggaagg gagctggcca
    17101 cactgacctc agacttaggg tacagaactg gatgtttaag ctgtgcttgt ttttttctac
    17161 ttatatagtt caggataccc tgtccaagaa atagtattgc caatggtggg caggctttct
    17221 gaactccaca taatcacaat aatttcccat agaaagcaca cagaacaacc tgatacagat
    17281 attcccttac tgagactctt gttaggtggt tttagattgt gtcaaattga aactaaccat
    17341 cacacctatt atattgaata tactcttgtt ggtagaagcc gagatttaac aaatacatct
    17401 tttacttttt ttgatagatt taaaagaaat gtgccttagt gccttggcga atttgacgtg
    17461 gcagtctcga acacaggatg aacatccaaa aaccatgttc tccaaagata aggtagagtt
    17521 ggaattaatg tcattgcagt gtactgaaga atccagtaga acttctagca gtcctcttta
    17581 gcaagtacgt tctcagtttt atgagtaaat cgtgtagtag ttatctttct tttttccata
    17641 tgtagaaatt ataatttacc agttatttag tagatgtcat ttggcttagc tcttagtatt
    17701 atcagtcctt aaacgcaaat tggttggctt tgtttgtttg tttgtttttg tttttttgtg
    17761 ttttgttttg ttttgtttcc gagacagggt ttctctgtgt agccctggct gtcctggaac
    17821 tcactctgta gaccaggctg gccttgaact cagaaatcca cctgcctctg cctcccaagt
    17881 gctgggacta aaggcgtgtg ccaccaccgc ccggctggtt ggctttttga agcaagcaag
    17941 tcatctatct cgtagccagg tccggtgtaa accagtctct agccttggct ggcctggaac
    18001 tgggatcttg aagctcatga tctgctttag ccttcctgtt tcaccttgtt tcagaaccca
    18061 aagcctctgg ccttgattct ctgtaaggtc acatgggacg cgtccttatc ctataggtac
    18121 aactgtctca gccttccctg gaaagagcac gaagatgcct ggctgcctgg tttctgacct
    18181 catccttatc ccagtgggga aaaaatgcta actggccaga tgagggccat catgggcagt
    18241 aggcctgaga ctgaaggcag gcttataaac actgaagagc taaaatctat atatactcac
    18301 ttgaaatctc cataatctgc atatttggtt ggtgggttgg ttgtttgaga cagagttttt
    18361 tactctattg ccctggcctg gaactcctag ccacccaccc tccggcttta gcctcccaag
    18421 tgctgaaact gttaagcctg cgccacgctg caagccatat gttattttga acaattgaat
    18481 attccttcag tacctaaaag ctacaggcag agttttattt tgattgtaat aaaatttttg
    18541 aaaagtgtgt gtgtgtgtgt gtgtgtgtgt gtgtaaaact ttgaaaacaa ctgtaattct
    18601 taatatattt atttcttaaa aataggatat tataccattt attgataaat actgggaatg
    18661 tatgacaacc agacagagac ctggaaaaat gacttggccc aataacattg tcaaaacaat
    18721 ggtaagtaaa ttaaaattga atagatatga tcagcttgaa aaagccagta ttgtttctaa
    18781 tcaataaatt ctctttagtt tgaagtaata ggatggctga gactatgcaa gcaatttgat
    18841 attaatcatt gaatgaatgg gattatggcc attatttaat tttttataca aattcagtta
    18901 gttactgcct tgtgtgaata taattctcta agactgcttt tatgtgctga aacccttgtc
    18961 tttttttttt ttttttttta agatttgttt attttatatg tatatgcttg agtgtgtgta
    19021 tgtgtaccta tagagaccag aaaggggcat tggatccctg gaattggagt tagatggctt
    19081 tgagtcacct gatattgatg ctgggaacta aacctggatg ctctggaaag cagcaggtgc
    19141 tcttaagcac tgagccatcc ctctggtcca gccatctctg tccaccacac ttttgcagat
    19201 ttaattttaa attaaatttg gatacatttt taaatttttt cattagataa gaaaagtctt
    19261 gttctgaaca ttgactttag aagtctgctt aacccaggca agaggagaga ggagggttgt
    19321 ggggcactgg ccctcactgt cctgttggtt atttttaggt ctcttgtagc ccaggcattg
    19381 aactcagtgt gtagccaagg gtgaccgacc cccggccttc tccctgcaga gtgccagtac
    19441 agttgtaact catgtaactc attgtctctc ctcttgcctc tgtgttcctt gtgctggcct
    19501 tccgtgtgga gccgccatgc ttggtgttct tgtggtgcta gggctcacac tgaggtcttc
    19561 ctgcctccca gcctgaccgg gctctgagtg ctctcacttg acttgttatg gtctgcactg
    19621 attttctgat gggtttggag agtacagtta gacattgaca ttggcagttg gtgagtgagt
    19681 atccccacac ttgtgagtaa aatgctcatt cttgctaaga tgccatttcc ttttagagta
    19741 aggaaagaga tgtgttcttg gtaaaggaac accctgaccc aggaagcaaa gacccagaag
    19801 aagattaccc caagtttgga cttttggatc aggtacatta atcttccttc tttgtctctc
    19861 tgtctctctc tcccgtatga ggtctgtgca ttgtgtttgt gtgtggctgc agaggccaga
    19921 cgagggagtg gagcagagct ctaggaaccc gagttacaga cacttgtgag ctccctgcag
    19981 tgctggggac tccaagctgg gactgctggg agagcagcag tgctcctagc cactgagcca
    20041 tctctcctgc cccatagtct ttttttttta atcagaagcc atttaggggc ggtgaagcat
    20101 gtggccaggg atgtggccga gttgagagca gtccttgcct gggagtcctg gtgtgttcat
    20161 ggtgatgcag tgaggaggtg caaacaagga agagcacgag tttgaggcca gcctgggcta
    20221 catgaggcct tgactcaaat aaaaagtgtt caagccttaa aataagagtt catatcccca
    20281 gctccccggt tcccaggagt tagtggtgat ctctttgtca caggatctca ctgtagccca
    20341 ggttagctta aactcaagac tgttcttcct gccttggctc tctctgctgg ggttacgtgt
    20401 gtgaggcgca gtgcctggct caggaaccgg ttttgattct ggttgttcct cggtgttagg
    20461 gattactttt tgtaagtttt tttctaatct tcctgttatc tgaaattttg cttctaaaag
    20521 cattctttag tgtgaagaaa tttctgggga atctgttgtt agatccttat gggaaaaaga
    20581 aatttccatt ttttgttgca aaggacacat tggcaactaa gaagatttgc cttaaagtgt
    20641 ctgacttgct gccttttttg agatgagatc tgtcagtgta gcatgacact gtggggacct
    20701 ctgtcctcag ccaccttccc agtcgctgag gtgggagctc actgctaccc atgcagattg
    20761 aagagctgcg gtgcctctag gtggtcactg ttatagcatg gacagtgaag attcaggttg
    20821 ctgtcacata cagtgggaca gctcagctgt gagggcagag tgaggggact tcatcgtcct
    20881 ggcctgctgt gcagctcccg cctaccggtt gattggtcag ttccctcctg gaaccattct
    20941 gaccctgact catggcacat gctccttcgt gagttgttga agcatccaag ttgaggacct
    21001 tgcagcttgc tgtttaggac atgactttga ttttctgtgc tctaattttg tccatttttg
    21061 gagcagttgc cagattaatt tgctcatgtg gccttttttt tactaggata tttcttcttc
    21121 taggatctta gtaatattgg tcctgcttat gacaaccaga aacaaagcag tgctgtgtct
    21181 gctagtggga acctaaatgg tatgtgttta agtatcttgt cagaagcttt gtgaatgatg
    21241 aaagccttgc ctgcaatgtg gtgctctgta ggaggagcca aacctgggta attactaaag
    21301 tgtttttaaa ttgacatgca tactgta
//