LOCUS       KO-first_condition_ready_38738_MGI:1341799        23051 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Ndrg1 targeting project(s): 38738
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 94525
COMMENT     backbone: L4L3_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(4153..4190)
                     /note="Transcription Terminator"
     misc_feature    3826..4079
                     /note="BGH pA"
     CDS             3045..3701
                     /note="DTA"
     promoter        2519..3031
                     /note="PGK promoter"
     promoter        complement(2368..2518)
                     /note="spectinomycin promoter"
     CDS             complement(1357..2367)
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     GK"
                     /note="SpecR"
     misc_feature    complement(617..1209)
                     /note="pUC ori"
     misc_feature    complement(319..346)
                     /note="rrnB T2"
     misc_feature    complement(144..187)
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8919..9039
                     /db_xref="ENSEMBL:ENSMUSE00001217616"
                     /rank="5"
                     /note="ENSMUSE00001217616"
     exon            6987..7092
                     /db_xref="ENSEMBL:ENSMUSE00001241456"
                     /rank="4"
                     /note="ENSMUSE00001241456"
     misc_feature    4275..10179
                     /note="5 arm"
     misc_feature    10207..10231
                     /note="B1 Gateway"
     misc_feature    10244..10291
                     /note="Frt"
     intron          10292..11166
                     /note="mouse En2 intron"
     misc_feature    11164..11166
                     /note="Splice Acceptor"
     exon            11167..11339
                     /note="mouse En2 exon"
     misc_feature    11351..11944
                     /note="ECMV IRES"
     gene            11945..15005
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    15044..15287
                     /note="SV40 polyadenylation site"
     misc_feature    15291..15324
                     /note="loxP"
     promoter        15351..15870
                     /note="human beta actin promoter"
     gene            15896..16690
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16890..17131
                     /note="SV40 polyadenylation site"
     misc_feature    17148..17195
                     /note="Frt"
     misc_feature    17202..17235
                     /note="loxP"
     misc_feature    11181..11258
                     /note="FCHK std_gpr"
     misc_feature    17251..17271
                     /note="B2 Gateway"
     misc_feature    10180..17301
                     /note="Synthetic Cassette"
     exon            18488..18548
                     /db_xref="ENSEMBL:ENSMUSE00001281904"
                     /type="targeted"
                     /rank="7"
                     /note="target exon 1 ENSMUSE00001281904"
     exon            17714..17776
                     /db_xref="ENSEMBL:ENSMUSE00001231824"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 2 ENSMUSE00001231824"
     misc_feature    17302..18706
                     /note="Critical Region"
     misc_feature    18730..18763
                     /note="Downstream LoxP"
     misc_feature    18707..18786
                     /note="synthetic loxP region"
     exon            22324..22427
                     /db_xref="ENSEMBL:ENSMUSE00001261056"
                     /rank="10"
                     /note="ENSMUSE00001261056"
     exon            20502..20558
                     /db_xref="ENSEMBL:ENSMUSE00001236962"
                     /rank="9"
                     /note="ENSMUSE00001236962"
     exon            19419..19505
                     /db_xref="ENSEMBL:ENSMUSE00001276013"
                     /rank="8"
                     /note="ENSMUSE00001276013"
     misc_feature    18787..23051
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatttaag
       61 actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag
      121 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc
      181 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
      241 atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
      301 ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
      361 ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc
      421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
     1321 cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat
     1381 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1441 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1501 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1561 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1621 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1681 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1741 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1801 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1861 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1921 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1981 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     2041 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2101 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
     2161 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2221 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2281 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     2341 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     2401 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     2461 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagagct gtttatcgaa
     2521 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     2581 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     2641 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     2701 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     2761 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     2821 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     2881 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     2941 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     3001 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg
     3061 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac
     3121 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa
     3181 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat
     3241 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt
     3301 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag
     3361 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca
     3421 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt
     3481 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga
     3541 ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat atggctcaag
     3601 cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg
     3661 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     3721 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     3781 ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg atcagcctcg
     3841 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3901 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3961 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4021 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4081 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgagttt
     4141 aaacagataa acggaaaaaa accgggccaa tgttggcccg gtttttttgg atttaggtga
     4201 cactatagat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggattgtac tagtgaatct atgtgcgttc ttttatccgg gtgtgctata
     4321 ctagtacatc catgtgcact tctgtgcaca tacagacatt tgtgggtact attcactagg
     4381 acatcagtgt gtactgttct atttaagact agtatatcca tgtccatgtg catgcagtgt
     4441 tgtggtattc tatttatgtg tatcatgtta ccagtattat ctatgagcac tgctattcca
     4501 ggagatctgt tattcgtaca tccatgttta ctgctgtgct tgtacatcca tgtgcattgc
     4561 tgtaattaat tcccacctgc tcgactgcta caaacatgtg ttctgccatt tttgttacac
     4621 gcatgaggac tggtatgcaa gtacacccat gtgcactgtt gtatgtgtac gaatggctgt
     4681 ctgtgctgca gttgtgcccc agttggggaa tccctgtcct catgggcttt acagccttat
     4741 tctgaccacc ccaagtcttg taggatggag gctgttcatg tgacaggtta gggtgacttt
     4801 tccagaacac agctcctgag tgggtggccc tccctatcta ttcttctctc acattagact
     4861 atgggtgctg cctctcctac ctcatgagac acatgggtcc tagattctgg aaggatgttt
     4921 ttggcacctg aaattttctg tgcctgtgtc acctgcatga ttctgaccac cagttgtagc
     4981 ttggaggtgt cacacagccc tttagggctt ttgcaagaga tgaaggatct ggagagaaat
     5041 ggcccagcta cacggaagtc ctcagtgtcc ccagcagacc tcgggagggc agccaagagc
     5101 caaccacagc agaggaggaa gctgttgctt tcagacgagg aaattgctcc tgggacatgc
     5161 ttcctcagaa gcctagagtg acagcaaaga ggacaggcac caagggccaa aggcagggtc
     5221 acagagggtc atagatgttc acaaccggct tcatgggagg ttcatttgac atcgggcagg
     5281 gtaaatgagc cttctgggag ctgaggactc ggaacgaagg tgaagcctgt ctttttgagg
     5341 gtagaagaaa gagacatatt ccttgttctc tcaagtgagc atggggggtg tgggggtgct
     5401 gtggattgct aagaactggc ttcagagtag agcaggagag agaaggaatt tagggggatc
     5461 gttgcaggtt ctctgctcaa tccagaatgg gaagttgttg gcccattaca ctgacagtcc
     5521 caggaagtag aggccatgct gagactaatg cctcctttct gtctttatca accccaggat
     5581 gatggtttaa tgcaggcaaa tatttgccta ttggctgcta gttaaacatt gctaggtcaa
     5641 gcccagcccc acttccacag tgagtcaggt tagctccact tggtatcacc taacagtgcc
     5701 tggcatttga gaggtgcaat gtgagaattg gcctcagtct gtgaaacaga gtgtgtcaag
     5761 ggagctggtg tttgtgtgtg tgtgtatgta tgtatgtatg tatgtatgta tgtatgtatg
     5821 tatatatgtc ttagtagctc ttctattttg tgaagagaca ccattacaaa ggtaactatt
     5881 taggaaaaaa aaacacatgt aattgcagct tgttaacaat ttcagttata tcctggcaag
     5941 gagcatggca gcacaaatgg tggtggagca gtagctgaaa tctatatctt gacccatggc
     6001 tgaggtgggg aggggagaag gaaaggtgaa cactgggcca tgtatgggct tctgaaaacc
     6061 tcaaagccca cccacagtga tgtacttctt ccaacaatct taattctaac cctatcagag
     6121 ctcccttctc tgctgactaa gcattcaaat atatgagcct gtgggagcca tttttattca
     6181 acccacccat tgcattgtgc tttgccacag gaaactgagg ttgttctttg aaatggggag
     6241 tgacttctag gaagcctcaa agagcaaata cctattatcc atgggataag tgagacacag
     6301 gaacaagaag tcaaggaaac tcaagccaga tgcagacaac agagtacttt tcaggcaatg
     6361 tgactggggc cacctgtctc tggattgtag cgcataacct gccagggtcc tgtcttctac
     6421 ctcctgatac agctttgctc tccttacctt ttcattttcc cttcccctct tctctccttc
     6481 ttcttcccct ctcccttcct tttctttcct ctttctcccc ccttctcctc cccctcccac
     6541 ttcacattcc ccttcccttc ccttcccttc ccttcccttc ccttcccttc ccttcccttc
     6601 ccttcccttc ccttcccttc ccttcccttc ccttgctctc tcctcctctc ccctcctctc
     6661 cccttccctt tcattatctc cccttccctc ccctcccctc cccttccctc ctcttccctc
     6721 ccctcccctc cccttccctt cccttccctt cccttccctt cccttccctt cccttccctt
     6781 ctctttcttt tcctttcctt ccctttctct tctgatctca gacatctgct gttgccagtg
     6841 cagagctcta gagaccatag gaggtgtgta cttggggtat cctcagaaca cagttgggag
     6901 catggcatgt gtccatagtc agtgggtcag ccaccctaaa actgtgaatc ccaggtgatg
     6961 ggcctctgtc tgtggtctct cctcaggagc aggatattga gaccctacat ggctccctgc
     7021 atgtcaccct gtgtgggacc cccaagggca accgtcctgt catcctcacg tatcacgaca
     7081 tcggcatgaa ccgtgagttt gggaagctgg aatgcttggg gagggctggc ggaagtcagc
     7141 tgctcttact gcttttggga cttcttgttt ccagcctcag cctgggaaaa cctgaaacat
     7201 actctgctgt tagtactgga tgctctgggg acaggaagcg agccccactg ctgcctaaca
     7261 agtgttacaa tgacttaaag attaatatat gcaatggcct ttgatactgt agtcattctg
     7321 aaaagaagcc ttgtttctgt tctccactgc cctgatcttc catattcccc agccctgctg
     7381 aggacacctc gtctcctcta tgtctctaga atgtcataca agcacagtcc tccagtgtgt
     7441 aatccctcat gattagctca tgaccagctt agtccatgtt ttcaagactg atatatgttg
     7501 tagctttgtc aatactttac tttcttgatg gtgaataaga ccctactgaa tggaaaccac
     7561 acttggtctt ccattttttc ctctaacaca tagcattatc agacctgtca cagcaatacc
     7621 tcacttctag taccaatttc tgtcttagtc actgttctac tgctgggaag agacaccatg
     7681 accagggcac ctcttaccaa agaaagcatt taattggggg catgcttata gtttcaaagg
     7741 tttagtccat ggtcaatgac agaaagcata aatcaggcag gtgctggaga agtacctgag
     7801 agctacatcc tgatctggaa gcaaagagag agagagactg ggcctggcat gggcttttga
     7861 aacctcaaac tctgcccctc agtgacacaa ttcttccaac aaagctatac ccaatccttc
     7921 taatcttttc aaatagtgcc actccctgat gactaagtct tcaaatacat gagcctatgt
     7981 gtgtgtgtgt gggggggggc attcttattc aaaccaccac actggaggat ggatactcag
     8041 ggtctttgca cttacctgta ttataaacag tgttgcagtg aattttcacg cagtggattt
     8101 ttaagttgaa agggagtaag ccaggcttga tggcttatgc atatcctctt agcacttggg
     8161 aagccaagtc aaggggattt ctgtgagttg gaaatcaacc tagcctacat aagttacagg
     8221 tcatccagag ctacagaaaa caaacaaaca aacaaacaaa caaaagtcaa gtgtggtagt
     8281 agtgttgcat ccctctctaa tctcagcagt catgggtagt ggtaagaaga acaggagttc
     8341 aaagacagcc tcaactatat agctagaggt ctttgcctca gccccttaac aaatgggaga
     8401 cttatcctag gagacacagg tagatacctc ttgctttacc taacatactt tttgtttgaa
     8461 aattatgtgt gccttgaaag atgtcccaga tcttaaagcc tgagagaccc agagaagttc
     8521 aggagtggat acatcttgta ctatccccat ggcaaccttt ccagagagga gacagagcca
     8581 gaccctcagc cacttctggc actggctgca aattgatgat ttaagaagct taggggctag
     8641 ttctacactg caccagacat tagttctatg actcttcctt cctctatcac cagagggagg
     8701 tctggtagga aggggtgaag acttatacta tttcttttta agacagagtc tcattataca
     8761 gtgaaacttg tctagaactc catgggtata gatcaggctg gcctcgaact cacagagatg
     8821 cacactttct tgggagcccc tctgctgctg ctacatgaag aaaaaaaatg agtgtatacc
     8881 taacatcttt ctcatttgcc ccttggtgtg tcctgcagac aagacctgct acaaccccct
     8941 cttcaactct gaggacatgc aggagatcac acaacatttt gctgtctgcc atgtggatgc
     9001 ccctggccaa caggatggtg ccccttcctt cccagtgggg taagagcaga gaccttcacc
     9061 ccacaagcag atgaggtgca tgtagaggaa gaaatgccag cctgggtaaa acaaaattat
     9121 ccatggtaaa agactagatt tgccatctct aagcagggca cagggatgca tgcctggacc
     9181 cccaacactt gggaagcttg aggttagctt caagtcatag caaaatcata ttaaattttt
     9241 ttaaaaggaa aaggaaagaa agaaaaaagc caagacaata tatgtgaatg tatcataaca
     9301 tttactttta aactagtttc ttttctattt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     9361 gagggagagg gagagagaga cagagacaga gagacagagg gaaagagaga gtgtgcatgc
     9421 atgcatgtga acatttatac tgtgcatctc acatagtctg cagctagggg gttgggctcg
     9481 ctgactctca taatatctca tgcaggaggt ctcctccaac actcgatgat agaacataca
     9541 ggaatagccc tttccctggg tttacgggct ttctgtggtg tccccaggac catttacaga
     9601 cacatgtaat cttttattac tgatctcagt tcttaattat taacttttat catcaaagaa
     9661 gagaagggac atctggctcc tcagggacag gtccaggttt aaagagcttg ttcaacagtg
     9721 gggtacagtt aaccgttaac cagctgacaa aaccagggtt ctctaagtca ctctgagact
     9781 ccacttagtc tgagagatgg gggggatgca gaggctctta catgttccta aaccttgcgc
     9841 cccccccccc cccagagata ggtgaagtgt atttgtagat taagatgtga ggtgtgtgct
     9901 cccaagttta actggtgact ttagggtgct taaaatagaa cactcttgga acagaatgtg
     9961 gcttctccct gccctctcgg gtagggtctg tgtgacaccc cttgtcttgg ggctatcaaa
    10021 gcaggattag ggatcaatgg ctgtcttctc ctctttggag tcttcttcct gcttaggaat
    10081 cagccactct cttttttttt aaaaatatcc atctccagga agctaacagg aaactgaagc
    10141 gttcatagtt aggtgacttt ttcattattg tacctgggaa aggcgcataa cgataccacg
    10201 atatcaacaa gtttgtacaa aaaagcaggc tggcgccgga accgaagttc ctattccgaa
    10261 gttcctattc tctagaaagt ataggaactt cgaacccttt cccacaccac cctccacact
    10321 tgccccaaac actgccaact atgtaggagg aaggggttgg gactaacaga agaacccgtt
    10381 gtggggaagc tgttgggagg gtcactttat gttcttgccc aaggtcagtt gggtggcctg
    10441 cttctgatga ggtggtccca aggtctgggg tagaaggtga gagggacagg ccaccaaggt
    10501 cagccccccc cccctatccc ataggagcca ggtccctctc ctggacagga agactgaagg
    10561 ggagatgcca gagactcagt gaagcctggg gtaccctatt ggagtccttc aaggaaacaa
    10621 acttggcctc accaggcctc agccttggct cctcctggga actctactgc ccttgggatc
    10681 cccttgtagt tgtgggttac ataggaaggg ggacgggatt ccccttgact ggctagccta
    10741 ctcttttctt cagtcttctc catctcctct cacctgtctc tcgacccttt ccctaggata
    10801 gacttggaaa aagataaggg gagaaaacaa atgcaaacga ggccagaaag attttggctg
    10861 ggcattcctt ccgctagctt ttattgggat cccctagttt gtgataggcc ttttagctac
    10921 atctgccaat ccatctcatt ttcacacaca cacacaccac tttccttctg gtcagtgggc
    10981 acatgtccag cctcaagttt atatcaccac ccccaatgcc caacacttgt atggccttgg
    11041 gcgggtcatc ccccccccca cccccagtat ctgcaacctc aagctagctt gggtgcgttg
    11101 gttgtggata agtagctaga ctccagcaac cagtaacctc tgccctttct cctccatgac
    11161 aaccaggtcc caggtcccga aaaccaaaga agaagaaccc taacaaagag gacaagcggc
    11221 ctcgcacagc cttcactgct gagcagctcc agaggctcaa ggctgagttt cagaccaaca
    11281 ggtacctgac agagcagcgg cgccagagtc tggcacagga gctcggtacc cggaagatct
    11341 ggactctaga gaattccgcc cctctccctc ccccccccct aacgttactg gccgaagccg
    11401 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
    11461 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
    11521 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
    11581 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
    11641 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
    11701 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
    11761 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
    11821 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta
    11881 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttgccac
    11941 aaccatggaa gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
    12001 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
    12061 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt
    12121 tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac
    12181 tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt
    12241 gacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta
    12301 ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt
    12361 tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca
    12421 ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg
    12481 cctcgcggtg atggtgctgc gctggagtga cggcagttat ctggaagatc aggatatgtg
    12541 gcggatgagc ggcattttcc gtgacgtctc gttgctgcat aaaccgacta cacaaatcag
    12601 cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga
    12661 agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg
    12721 tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg
    12781 tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc
    12841 cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat
    12901 tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct
    12961 gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca
    13021 tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga agcagaacaa
    13081 ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga
    13141 ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca tggtgccaat
    13201 gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat
    13261 ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg
    13321 ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc
    13381 ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta
    13441 cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg
    13501 gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc acgcgatggg
    13561 taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca
    13621 gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa
    13681 cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat
    13741 gaacggtctg gtctttgccg accgcacgcc gcatccagcg ctgacggaag caaaacacca
    13801 gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct
    13861 gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct
    13921 ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc
    13981 tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc
    14041 gaacgcgacc gcatggtcag aagccgggca catcagcgcc tggcagcagt ggcgtctggc
    14101 ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc tgaccaccag
    14161 cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg
    14221 ctttctttca cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca
    14281 gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc
    14341 taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt
    14401 gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca
    14461 gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca
    14521 aatggcgatt accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg
    14581 cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca
    14641 agaaaactat cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc
    14701 agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga
    14761 attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag
    14821 tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag aaggcacatg
    14881 gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt
    14941 atcggcggaa ttccagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa
    15001 ataataataa ccgggcaggg gggatctaag ctctagataa gtaatgatca taatcagcca
    15061 tatcacatct gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
    15121 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
    15181 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    15241 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgga ataacttcgt
    15301 atagcataca ttatacgaag ttatgtttaa acggcgcgcc ccggaattcg ccttctgcag
    15361 gagcgtacag aacccagggc cctggcaccc gtgcagaccc tggcccaccc cacctgggcg
    15421 ctcagtgccc aagagatgtc cacacctagg atgtcccgcg gtgggtgggg ggcccgagag
    15481 acgggcaggc cgggggcagg cctggccatg cggggccgaa ccgggcactg cccagcgtgg
    15541 gcgcgggggc cacggcgcgc gcccccagcc cccgggccca gcaccccaag gcggccaacg
    15601 ccaaaactct ccctcctcct cttcctcaat ctcgctctcg ctcttttttt ttttcgcaaa
    15661 aggaggggag agggggtaaa aaaatgctgc actgtgcggc gaagccggtg agtgagcggc
    15721 gcggggccaa tcagcgtgcg ccgttccgaa agttgccttt tatggctcga gcggccgcgg
    15781 cggcgcccta taaaacccag cggcgcgacg cgccaccacc gccgagaccg cgtccgcccc
    15841 gcgagcacag agcctcgcct ttgccgatcc tctagagtcg agatccgccg ccaccatgat
    15901 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
    15961 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
    16021 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
    16081 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
    16141 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
    16201 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
    16261 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
    16321 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
    16381 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
    16441 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
    16501 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
    16561 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
    16621 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
    16681 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
    16741 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
    16801 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
    16861 cccccggatc taagctctag ataagtaatg atcataatca gccatatcac atctgtagag
    16921 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
    16981 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    17041 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    17101 ctcatcaatg tatcttatca tgtctggatc cgggggtacc gcgtcgagaa gttcctattc
    17161 cgaagttcct attctctaga aagtatagga acttcgtcga gataacttcg tatagcatac
    17221 attatacgaa gttatgtcga gatatctaga cccagctttc ttgtacaaag tggttgatat
    17281 ctctatagtc gcagtaggcg gtcccatgtc tttgcccctc ctttctccta aaggtgtctc
    17341 cagttaagta aagtcacacc atccaggctc ctcccagctc tttcagggtc ctccacgtat
    17401 cgtctctgtc tagggtccat ttctctactg ggctctgtct cagcatcact tcctttagga
    17461 aacccttcct gaacagttga acaaagtgga tccccttgct tctatgtcct tacagcctat
    17521 cactgtgcct tctcagttca gagctacagt gttagggtgg ggtatgctta cccagtggat
    17581 ctgaggggtg gggttgtaac gtctctgtgt gcaagggcct gctggtacct gagctttcag
    17641 aatatcctgt atgggcgatt tgtttctccc agggtctggg ttctcaagat ctgcctcccc
    17701 atatacccta caggtacatg tacccctcaa tggatcagtt ggctgaaatg cttcctggag
    17761 tccttcacca gtttgggtaa gagcaaagga cccttttttc caaggttgtc aggaaagggc
    17821 agctgctgac ctccttctct accttgacct gactactaaa gtgcaggttg gagttctcag
    17881 tcaggtctgg aaaggaactt gctacagctt gtactcaccg tttcctcaac tggacattgt
    17941 gctctgtgtc ctgtcactgt ggccttgctt cttgagtgac ccccaatgca cattctgtcc
    18001 ctgcctcctg atcctatgga acattctgga agagtgttcc ctgggatctt ggcttctctt
    18061 tccagaccct cattctgccc ctgcaggctc ccctttcttt ctcacttcag cctctccttg
    18121 ggtggaactc aatggttctg gcccaatttg ttctcattcc aagtttcaga atccagaaaa
    18181 aagcttggaa gtggggtgag gcaacttctt aatgggcctt cctgtaaatg ctaggcgtct
    18241 cagagcctgg gctggccaca gtcaggggaa aacgagcaag agggttgagg ctttcaaggc
    18301 cccagctgtg gacctgcagg gcactcaggg cagcctgcct ctcatggcat gggcaggctt
    18361 gtcagcatcc tcaagaatga catgcaaggg gtggagctct caggagtaag tgaggaaagt
    18421 ttataggagt gggcaggggc tccatcacat ctccctttgg cgacctaaat cttcatttgg
    18481 tttccaggct caagagtgtc attggcatgg ggacaggagc tggcgcctac atcctgaccc
    18541 gcttcgcagt aagttctttg gcagtttgtg aagtgacatt aaaaatgatt taggggccgg
    18601 ggagctagct gagtgggcaa gagtgtgccg tgcaagcatg gagagttgag gactggagtt
    18661 ggaatcagaa cccgtataaa agacaggcat ggctgcaggt gcctttgaga tggcgcaacg
    18721 caattaatga taacttcgta tagcatacat tatacgaagt tatggtctga gctcgccatc
    18781 agttcaacta acagctttgg tctttttggt gctgaaatgc cacactagat agggcttttg
    18841 atcactggtc ctgttccctg aaccagaaat gtggatggct tctcactcat agagacctct
    18901 gatttttgca ctgttttatc tctgagtttt gtgacccagg tcatctggaa ttcagaatta
    18961 agtctaaatc agagtcagac atgtgctagt gtgacagtga ccactgctag cactgtcttt
    19021 accagttatc ttaaagaggt gcatgccctt cagcccaagg tattttgatg cttagtaagt
    19081 gtcgcacctt aatggtgccc aggcgtaccg tcaacctggg catccgtgtc acctagatgc
    19141 tagcatggtt ttggagtcct aaatacatag gaattccagg aatcgagcca cagggtatct
    19201 ctgtgccctt ctcacttagt tgtggcttgg ctattgcaca ctgggcattc tttctttttc
    19261 tatggcttct tcctcctgct ctcttgaggg cctagtccag ttttgaatgg aatctcagtt
    19321 ctgcatggac tatgcaggag ccattaacat ctgctctcag gtccccacag ttgctaacat
    19381 agccctcagt atatgtcatg ttgtgtctct gattacagct caacaaccct gagatggtag
    19441 agggtctcgt gctcatgaat gtgaacccct gtgctgaagg ctggatggac tgggctgcct
    19501 ctaaggtaag ggcggacaga gaagattggt ggggtcccag cctctactca gggtagcacc
    19561 ttcttgcccc cacccccgcc tctttcctta ctgtccaaga gagtaccctt cctcagccct
    19621 ttttaggcta gcaaccattt gctgctctca aaggtcttag aggtttcgcc ctggctgctg
    19681 tcccaaggaa ccctgttgat cctgttgatt tttttttcct aagtgaggat tgctttcttt
    19741 ttttctgtaa agtccccatt gtctgccaag gatagggaga aatttctgat taaagccaca
    19801 caggctaatg tgacaagtct cttactggga tagtagtttt cttaggcagc tgttggggaa
    19861 aagggcatgg gctacaccta tcccttgctg tctgctagat gctgtgtttc ttcctaaagt
    19921 cttgatataa tttgggagcc ctgtccatcc ctgtagggca gccaggaatg aagaatcctg
    19981 cctggctttt attttatttt ccagctgaga aagggacatt ccatttgggt ccacttggct
    20041 gctggtccac tgttctaagc caaacaagga aggctggggc actggcctcc agctgagaga
    20101 taggctgtgg ttttaatgaa ttccccatgt gaagtcaaga cagcctgcat atctaaggcc
    20161 attggtacca aaaatgagta tcagagcctt tgatgcctgg tgacttacag gatccttact
    20221 atggcctgat gtcacggttt ttaatacctt cagttaaaaa caaaagacct gagatctagg
    20281 gccttcagcc cccactccat ctccagtgct ccacagctgc aagtggccat tgggccagtc
    20341 caggacccca ggtcaagtgt tcatctccct tgcctatctc tacccaagca gaactacctg
    20401 tgttatctgc cttctatcta tagagcacct aggctgtctg actcttctct ccgctgttga
    20461 gtcctagttg ctgatttctt tccttggttc atcctccaca gatctcagga tggacccaag
    20521 ccctgcctga catggtggtg tcccacctct tcggcaaggt gagtggtgta gacctgtgtc
    20581 ctgtgtcctc acggagcagt taaccatgtg acactcagca cagagccaca tgggcatgtg
    20641 gtacaaatga gatgcagtca aggggtgacc caggctgaag tctgtgaatt attgtgaagt
    20701 attgactaat agccagcaag ccattcatat gatttttctt gtctctccca cattgagaat
    20761 tttatacatg tgtacaatgt tttctgaaca aatgtactct gagttctttc ccttcagctc
    20821 ttcccagact tccctcctga cttcatgtgt gtgtttgttt taaattacaa aaccctgctg
    20881 gtactgagtc tggtatgagc gagtatatag ggttatccac cagagcatga acaacctacc
    20941 tggggcacat tccttgctga aaactgatct ccctcctccc cttgagacac cttcattagc
    21001 tcctcaccag gggtggggtt tcatgataaa ctgttttttg ttgaagcaca gtgtgttgca
    21061 tatttgaaca aatctaccag tcatctcagc aactgagatg atggaaatgt ccttcaccct
    21121 tctcctccat gtcattgcac tgtgagccat cctcttctcg ttcctcccag gcaccctctg
    21181 ctctgctttt tgtccatcaa gtgactcctg tactcctctc tttgccatgg cttctgtccc
    21241 ttagcatgat tattttagaa tctactcatg tttttttgga caccataata gattcagtac
    21301 tgtgtaatat tccactgaat ggacatacca ttggtttcct gttgacctgt gtagggcctt
    21361 catttgtaac agtttggggt cattaggagt gaagtctgca gtgtacatgt ctgtgggatg
    21421 agctctgtgt ctgtgtgagg cactatcacc agttctttcc aaggattagc tcagggagcc
    21481 tgttcaggaa ctctctgacc atgaggtaaa catgacattc attccagtcc gagctgaaga
    21541 aattcagacc agaggttgaa tggcttgggt aaggtctcca ggagaccagg agaagtatga
    21601 ctgggatttg aacttgtaag tgtaactaca acatacagtc tcctaagctg cttcctttgc
    21661 ctgtgtctta catgagctga agacagaata ggcacccatg tagccatgtt gacgcaaggc
    21721 caacagccat gcgcgaggct gtccttttgg ctcacatcca agttcaatgc ctggaaaacc
    21781 cctcggttgt ccagacccag tgaagcactg cttactggat gtagcagtct ctgctgctga
    21841 gctaatgaac gtgacaacac ttgaatgccc tcttgagtct cagggcagca gaaaataccc
    21901 tgctttggtg taactccttg gcttgtagtt gaaatggctt agagcaacct gaggttatca
    21961 tcacacagtc cagactgtcc ccaagtccct tgaggctttg atgatgcccc caagttattg
    22021 ctgttaattc gaccagattc ctcagaccac tttcccccag aatgtctttg tcatggctaa
    22081 ggctccttac ttttgatccc ctaaggggca ggttgctctg aggtctacca tgaagcagga
    22141 gtcccgagtt tagctgaggc tcctgaggga aagctggttc tctggtacca gtagttatca
    22201 ctcagctgtc tgtaccagac tcactcagtt agttacccct gcctcatctg tgccttccct
    22261 tcttatctct gtggctggag aatggtgaca agccttcccc ctctcttccc tctgtcacta
    22321 caggaggaga tacacaacaa cgtggaggta gtgcacacgt accgccagca catcctcaac
    22381 gacatgaacc cgagcaacct acacttattc atcagcgcct acaacaggtg agagccctgt
    22441 gcaggcacag aagctgccag gtcacactgg ggtcctcatc agcttcctgc agcaaacctg
    22501 ggatggaagc agagagcagg actggccgag cagctgtagg ttgatagggt ggggcccagg
    22561 gaaggacaat ggccgtgtgg gagctgcctc gctctttgca ctgagtgagc tctgctggga
    22621 tctgagctcc cacccagact gtggggccag atgagtggga tgtagctgag tggtcagagt
    22681 ggagagctga ggagtgtgcc caaggctgac tcacaataaa agcccctgag agtggagctg
    22741 agcaaaggat agaaacattg taaggattca gaaccctaga ggctgctggg aggagaggca
    22801 gctgagggta aaggatgagg tacctgaggt ttcctggggg agaggactac acaaggatat
    22861 tagcatcagt cagaagttct agaaccttct agtgaaaacg tgtgaagtac ctcggccaca
    22921 ctcccaatac agcggccagc agtgagcctt gcatacagat ctgacctagg gcagtgccta
    22981 agacttaacc tgctggaact gagtttgcat ttgtaatgag tttaggttct ctgatggact
    23041 ctggtacctt t
//